AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1184400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1184400 MtrunA17_Chr8g0334461 92.042 289 18 2 2 287 1 287 2.94e-175 485
Msa1184400 MtrunA17_Chr8g0340031 60.455 220 84 2 67 286 33 249 7.24e-98 288
Msa1184400 MtrunA17_Chr8g0356021 46.512 215 111 3 73 287 49 259 2.12e-64 202
Msa1184400 MtrunA17_Chr8g0340821 43.519 216 116 4 73 287 51 261 1.12e-61 196
Msa1184400 MtrunA17_Chr8g0340811 43.519 216 116 4 73 287 54 264 2.35e-61 195
Msa1184400 MtrunA17_Chr8g0340021 63.077 130 48 0 157 286 23 152 5.89e-60 187
Msa1184400 MtrunA17_Chr6g0487381 43.578 218 117 4 70 286 39 251 8.33e-58 186
Msa1184400 MtrunA17_Chr8g0334471 98.592 71 1 0 159 229 7 77 2.88e-49 157
Msa1184400 MtrunA17_Chr1g0168451 37.615 218 133 3 70 287 52 266 4.33e-48 161
Msa1184400 MtrunA17_Chr1g0186301 36.364 220 135 5 68 287 48 262 1.10e-42 147
Msa1184400 MtrunA17_Chr7g0265641 38.739 111 66 2 160 269 6 115 6.54e-20 83.6
Msa1184400 MtrunA17_Chr8g0356651 43.182 88 46 2 73 160 52 135 1.67e-18 80.9
Msa1184400 MtrunA17_Chr8g0356661 57.407 54 23 0 231 284 1 54 3.63e-16 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1184400 AT2G38600.2 57.983 238 95 3 51 286 16 250 1.33e-94 280
Msa1184400 AT2G38600.1 57.983 238 95 3 51 286 16 250 1.33e-94 280
Msa1184400 AT4G29270.1 42.202 218 122 3 70 287 43 256 2.12e-62 197
Msa1184400 AT4G29260.1 43.891 221 120 3 66 286 38 254 2.56e-61 195
Msa1184400 AT4G25150.1 43.925 214 116 3 73 286 50 259 1.44e-60 193
Msa1184400 AT5G51260.1 39.506 243 139 4 44 286 22 256 2.08e-59 190
Msa1184400 AT1G04040.1 36.948 249 148 5 46 287 25 271 2.27e-50 167
Msa1184400 AT5G44020.1 37.273 220 134 4 70 287 55 272 4.80e-47 159
Msa1184400 AT5G24780.1 35.648 216 131 7 73 286 59 268 1.49e-39 139
Msa1184400 AT5G24770.1 36.111 216 130 7 73 286 54 263 2.08e-39 139
Msa1184400 AT5G51260.2 36.022 186 111 4 44 229 22 199 1.22e-37 132
Msa1184400 AT5G24780.2 32.298 161 103 5 73 231 59 215 3.28e-24 98.2
Msa1184400 AT5G24770.2 33.333 159 100 5 73 229 54 208 1.35e-23 95.9

Find 86 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATTGTCCGTAGTGATTT+TGG 0.138152 8_1:+20088842 None:intergenic
TCATCAAGATGCTTGGATTT+TGG 0.148705 8_1:-20089161 Msa1184400:CDS
AAGAGGAAGGGTATAGAATA+TGG 0.225208 8_1:-20085899 Msa1184400:CDS
AAAGCAGAATATATATAATA+AGG 0.234689 8_1:+20089626 None:intergenic
TTGTTAATTACCTTAAAATA+AGG 0.238981 8_1:+20088790 None:intergenic
AATCTCTTAGCTTTATAATA+AGG 0.250871 8_1:+20089109 None:intergenic
TATTATAAAGCTAAGAGATT+TGG 0.260805 8_1:-20089106 Msa1184400:CDS
TGCCATTGCCAACAAATTCT+TGG 0.268839 8_1:-20089665 Msa1184400:five_prime_UTR
GAAGAAGAAGAATCTAATTC+AGG 0.315145 8_1:+20089412 None:intergenic
ATTATAAAGCTAAGAGATTT+GGG 0.319914 8_1:-20089105 Msa1184400:intron
TGATCTCCAAGGGGAGTGTT+TGG 0.332481 8_1:-20085853 Msa1184400:CDS
TTCTTCGTCGTCGGAGATGA+TGG 0.343997 8_1:-20089392 Msa1184400:CDS
CTTAGTACCACAGGGTTTGC+AGG 0.358088 8_1:+20088936 None:intergenic
AATTTGTTGGCAATGGCATT+AGG 0.360678 8_1:+20089670 None:intergenic
TTCAAAGTCTTCTTAGTAAC+GGG 0.402138 8_1:-20088885 Msa1184400:CDS
TTGAACATCGCCGAATCAAA+TGG 0.405341 8_1:+20088984 None:intergenic
GAAATGCAGTACATAGGATT+GGG 0.410928 8_1:+20085810 None:intergenic
AATATATGAACAAATTGAAT+AGG 0.414480 8_1:+20089593 None:intergenic
AAGCAGAATATATATAATAA+GGG 0.423271 8_1:+20089627 None:intergenic
TATAGAATATGGGGAAATGT+TGG 0.423389 8_1:-20085888 Msa1184400:CDS
GATCTCCAAGGGGAGTGTTT+GGG 0.434280 8_1:-20085852 Msa1184400:CDS
AAAGATATTGCAAGCAGTAT+TGG 0.455653 8_1:-20089542 Msa1184400:CDS
CAAACAATAATGTGCGTCCT+TGG 0.456134 8_1:-20089318 Msa1184400:CDS
CGGAAGGAGATTGAAGAGGA+AGG 0.460525 8_1:-20085912 Msa1184400:CDS
CTAGAAGAAGATTATAAGAA+AGG 0.463752 8_1:+20089733 None:intergenic
AGAGGAAGGGTATAGAATAT+GGG 0.466203 8_1:-20085898 Msa1184400:CDS
TTTCTTACATGGATTGATGA+TGG 0.469031 8_1:+20089769 None:intergenic
ATTTGATTCGGCGATGTTCA+AGG 0.469405 8_1:-20088982 Msa1184400:CDS
AGAAATGCAGTACATAGGAT+TGG 0.475430 8_1:+20085809 None:intergenic
AAATGTGTCCAAGAATTTGT+TGG 0.483989 8_1:+20089657 None:intergenic
TGAACATCGCCGAATCAAAT+GGG 0.497521 8_1:+20088985 None:intergenic
CTACTACTCATCAAGATGCT+TGG 0.498659 8_1:-20089168 Msa1184400:CDS
CAGATGCGACCCATTTGATT+CGG 0.501157 8_1:-20088994 Msa1184400:CDS
TTAAACAATCTTAGTACCAC+AGG 0.502868 8_1:+20088927 None:intergenic
ATGAGAGGTATGGGCTGAGC+TGG 0.503269 8_1:-20089357 Msa1184400:CDS
CATGTTGAGAATTACATGAT+TGG 0.504365 8_1:-20089268 Msa1184400:CDS
ATTCAAAGTCTTCTTAGTAA+CGG 0.509040 8_1:-20088886 Msa1184400:CDS
GAATATATATAATAAGGGAT+TGG 0.510983 8_1:+20089632 None:intergenic
GTTGGTAGCCGATGAATCCT+TGG 0.512582 8_1:+20088814 None:intergenic
ATTCGGCGATGTTCAAGGCA+TGG 0.525698 8_1:-20088977 Msa1184400:CDS
GTTGATGATGATGAGAGGTA+TGG 0.526024 8_1:-20089367 Msa1184400:CDS
CCCCTTGGAGATCAGTCCAC+TGG 0.527498 8_1:+20085862 None:intergenic
TTCAAGGCATGGATTAATCA+AGG 0.531044 8_1:-20088966 Msa1184400:CDS
GTTGAGAATTACATGATTGG+TGG 0.532110 8_1:-20089265 Msa1184400:CDS
TGAGTAGTAGAAGTAGAAAG+AGG 0.532684 8_1:+20089181 None:intergenic
TTGCATAACCAAGGATTCAT+CGG 0.534552 8_1:-20088822 Msa1184400:CDS
TTTAAAACGTTGATAGACAA+AGG 0.537850 8_1:-20088909 Msa1184400:CDS
GGAAGGAGATTGAAGAGGAA+GGG 0.539212 8_1:-20085911 Msa1184400:CDS
ATATTGCAAGCAGTATTGGC+GGG 0.541832 8_1:-20089538 Msa1184400:CDS
TGAAATACAAGTCATCGATT+CGG 0.550970 8_1:-20085932 Msa1184400:CDS
AATCAAGAAATGCAGTACAT+AGG 0.552007 8_1:+20085804 None:intergenic
GATTGCCCAAACACTCCCCT+TGG 0.552792 8_1:+20085847 None:intergenic
GTATGGGCTGAGCTGGAGGT+TGG 0.553413 8_1:-20089350 Msa1184400:CDS
TTTGTTGAGTACTACACAAG+TGG 0.559383 8_1:+20085764 None:intergenic
GTCCAAGAATTTGTTGGCAA+TGG 0.560861 8_1:+20089663 None:intergenic
GATATTGCAAGCAGTATTGG+CGG 0.561358 8_1:-20089539 Msa1184400:CDS
GCTGAGCTGGAGGTTGGCAA+TGG 0.567591 8_1:-20089344 Msa1184400:CDS
TTAGTACCACAGGGTTTGCA+GGG 0.578209 8_1:+20088937 None:intergenic
ACCAGTGGACTGATCTCCAA+GGG 0.586615 8_1:-20085863 Msa1184400:CDS
CGAAGTGGGACTGTCTTCCA+AGG 0.589549 8_1:+20089301 None:intergenic
TTCGTCGTCGGAGATGATGG+TGG 0.596102 8_1:-20089389 Msa1184400:CDS
GGCAGGAGTGTGAAATACAA+AGG 0.596346 8_1:-20085966 Msa1184400:intron
ATTACCTTAAAATAAGGCGT+TGG 0.596763 8_1:+20088796 None:intergenic
GAGGAAGGGTATAGAATATG+GGG 0.605333 8_1:-20085897 Msa1184400:CDS
TTGATGATGATGAGAGGTAT+GGG 0.609061 8_1:-20089366 Msa1184400:CDS
TATTAAGCAATCTAGTGTTG+AGG 0.609828 8_1:-20089495 Msa1184400:CDS
ACATGCTTGTAGCAACGAAG+TGG 0.613933 8_1:+20089286 None:intergenic
TATTGCAAGCAGTATTGGCG+GGG 0.620877 8_1:-20089537 Msa1184400:CDS
AACACTTGCCAAAATCACTA+CGG 0.621427 8_1:-20088850 Msa1184400:CDS
AGATTATAAGAAAGGTACTA+CGG 0.623028 8_1:+20089741 None:intergenic
ACGGACAATTTGCATAACCA+AGG 0.630462 8_1:-20088831 Msa1184400:CDS
ATACAAGTCATCGATTCGGA+AGG 0.633877 8_1:-20085928 Msa1184400:CDS
GACCAGTGGACTGATCTCCA+AGG 0.635451 8_1:-20085864 Msa1184400:CDS
AGAGGTATGGGCTGAGCTGG+AGG 0.636934 8_1:-20089354 Msa1184400:CDS
CATGCTTGTAGCAACGAAGT+GGG 0.638112 8_1:+20089287 None:intergenic
GGGGAAATGTTGGAGACCAG+TGG 0.640511 8_1:-20085878 Msa1184400:CDS
AAATGCAGTACATAGGATTG+GGG 0.643105 8_1:+20085811 None:intergenic
GATTCGGAAGGAGATTGAAG+AGG 0.657286 8_1:-20085916 Msa1184400:CDS
ACACAATTTCATCCACGATG+AGG 0.660835 8_1:+20089221 None:intergenic
TAAACAATCTTAGTACCACA+GGG 0.665394 8_1:+20088928 None:intergenic
TTAGTAACGGGTAGATACGA+AGG 0.667985 8_1:-20088873 Msa1184400:CDS
TGGTGGTTGATGATGATGAG+AGG 0.675061 8_1:-20089372 Msa1184400:CDS
GAAATACAAAGGACAAAGTG+CGG 0.704065 8_1:-20085955 Msa1184400:CDS
CCAGTGGACTGATCTCCAAG+GGG 0.746364 8_1:-20085862 Msa1184400:CDS
GCATGACATGAACCTCATCG+TGG 0.754708 8_1:-20089233 Msa1184400:CDS
AATGTGCCCTGCAAACCCTG+TGG 0.782788 8_1:-20088943 Msa1184400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTATGATATGATAT+TGG - chr8_1:20086470-20086489 Msa1184400:intron 10.0%
!!! TTTTTTTATGATATGATATT+GGG - chr8_1:20086471-20086490 Msa1184400:intron 10.0%
!! ATATATATAATTGGAAATTA+AGG + chr8_1:20087290-20087309 None:intergenic 10.0%
!!! TAATTGTTAAAATTGTTTTA+TGG + chr8_1:20088074-20088093 None:intergenic 10.0%
!!! TTAACAATTAATTTTCTTTT+TGG - chr8_1:20088084-20088103 Msa1184400:intron 10.0%
!! TGTATTCAATTAATAAAAAT+TGG - chr8_1:20088480-20088499 Msa1184400:intron 10.0%
!! GTATTCAATTAATAAAAATT+GGG - chr8_1:20088481-20088500 Msa1184400:intron 10.0%
!! AAGCAGAATATATATAATAA+GGG + chr8_1:20085867-20085886 None:intergenic 15.0%
!! AAAGCAGAATATATATAATA+AGG + chr8_1:20085868-20085887 None:intergenic 15.0%
!! AATATATGAACAAATTGAAT+AGG + chr8_1:20085901-20085920 None:intergenic 15.0%
!!! TTTTTATTATATGTTAGACT+TGG - chr8_1:20086433-20086452 Msa1184400:intron 15.0%
!!! TTTTATTATATGTTAGACTT+GGG - chr8_1:20086434-20086453 Msa1184400:intron 15.0%
!! TTGTTAATTACCTTAAAATA+AGG + chr8_1:20086704-20086723 None:intergenic 15.0%
!!! TTATTTTTTTTTGTGATGTT+CGG - chr8_1:20086912-20086931 Msa1184400:intron 15.0%
!! TTTATAACAATGATATTTGT+GGG - chr8_1:20087013-20087032 Msa1184400:intron 15.0%
!!! TGTTAACTCTAAAATAAAAT+AGG + chr8_1:20087342-20087361 None:intergenic 15.0%
!!! ATATACTCTTTTTGTTTTAT+AGG + chr8_1:20087490-20087509 None:intergenic 15.0%
!!! TGCATTTTCTAATTTTATTA+CGG - chr8_1:20087607-20087626 Msa1184400:intron 15.0%
!! AACAATTTCATAATATCAAA+AGG + chr8_1:20088110-20088129 None:intergenic 15.0%
!!! TAAGCAAAAATTAACTTTTT+AGG - chr8_1:20088235-20088254 Msa1184400:intron 15.0%
!!! TAAGCAAAAATCAATTTTTT+AGG + chr8_1:20088324-20088343 None:intergenic 15.0%
!!! AATTTATTGAAAGTATAAAC+AGG + chr8_1:20088420-20088439 None:intergenic 15.0%
!!! TGTATTCAATTAATTTTTTG+AGG - chr8_1:20088454-20088473 Msa1184400:intron 15.0%
!!! GTATTCAATTAATTTTTTGA+GGG - chr8_1:20088455-20088474 Msa1184400:intron 15.0%
!!! TTTGTTAACCTAATTTTTAT+GGG + chr8_1:20088683-20088702 None:intergenic 15.0%
!! AAATTCGTAATTAACAATAA+AGG - chr8_1:20088775-20088794 Msa1184400:intron 15.0%
!! AATTCGTAATTAACAATAAA+GGG - chr8_1:20088776-20088795 Msa1184400:intron 15.0%
!!! AACTTTTTTTTTCTAACTTA+CGG - chr8_1:20088829-20088848 Msa1184400:CDS 15.0%
!! AGATAAGAAAATATTAGTAT+CGG + chr8_1:20089058-20089077 None:intergenic 15.0%
!!! CTTTTAACCTTAATATTTAT+TGG - chr8_1:20089498-20089517 Msa1184400:CDS 15.0%
!! GAATATATATAATAAGGGAT+TGG + chr8_1:20085862-20085881 None:intergenic 20.0%
!!! AATCTCTTAGCTTTATAATA+AGG + chr8_1:20086385-20086404 None:intergenic 20.0%
!! TATTATAAAGCTAAGAGATT+TGG - chr8_1:20086385-20086404 Msa1184400:intron 20.0%
!! ATTATAAAGCTAAGAGATTT+GGG - chr8_1:20086386-20086405 Msa1184400:intron 20.0%
!! TTTATTATATGTTAGACTTG+GGG - chr8_1:20086435-20086454 Msa1184400:intron 20.0%
!! ACAGAAAATTATTAATGACT+AGG - chr8_1:20086883-20086902 Msa1184400:intron 20.0%
!!! TATTTTTTTTTGTGATGTTC+GGG - chr8_1:20086913-20086932 Msa1184400:intron 20.0%
!! GTTTATAACAATGATATTTG+TGG - chr8_1:20087012-20087031 Msa1184400:intron 20.0%
!! TTATAACAATGATATTTGTG+GGG - chr8_1:20087014-20087033 Msa1184400:intron 20.0%
!! ACAGATAACTATACTAAAAT+CGG + chr8_1:20087141-20087160 None:intergenic 20.0%
!! CATAGCTTGATATATATAAT+TGG + chr8_1:20087299-20087318 None:intergenic 20.0%
!!! GATTTGGTTAAAATAATACA+AGG + chr8_1:20087447-20087466 None:intergenic 20.0%
!!! AAGAGTATATTTAATTGACT+TGG - chr8_1:20087500-20087519 Msa1184400:intron 20.0%
!!! ATATATCCACATTTTCTATT+GGG - chr8_1:20087692-20087711 Msa1184400:intron 20.0%
!!! TATTGGGATATTTTTTGTAT+TGG - chr8_1:20087708-20087727 Msa1184400:intron 20.0%
!!! AGGTGTTTTATTTTATTTGA+GGG + chr8_1:20087882-20087901 None:intergenic 20.0%
!! CAAAACTTACTCATTATATT+TGG + chr8_1:20088183-20088202 None:intergenic 20.0%
!! CATTCATTAAATGATGTATT+TGG - chr8_1:20088260-20088279 Msa1184400:intron 20.0%
!! AATTAATTATGAAGATTGCA+TGG + chr8_1:20088378-20088397 None:intergenic 20.0%
!!! ACAGGTTTAATTTTTTTTGA+AGG + chr8_1:20088402-20088421 None:intergenic 20.0%
!!! CTTTGTTAACCTAATTTTTA+TGG + chr8_1:20088684-20088703 None:intergenic 20.0%
!! CATAAAAATTAGGTTAACAA+AGG - chr8_1:20088682-20088701 Msa1184400:intron 20.0%
!!! AAAGGTTCAATTAATTTTGA+GGG - chr8_1:20088700-20088719 Msa1184400:intron 20.0%
!! ATTCGTAATTAACAATAAAG+GGG - chr8_1:20088777-20088796 Msa1184400:intron 20.0%
!!! CTCTAAAAGTGTTTTTATTA+TGG + chr8_1:20089120-20089139 None:intergenic 20.0%
!!! CATAATAAAAACACTTTTAG+AGG - chr8_1:20089118-20089137 Msa1184400:CDS 20.0%
!!! GAAATTAAGTTGATTAATGA+TGG - chr8_1:20089466-20089485 Msa1184400:CDS 20.0%
!!! ACCTTAATATTTATTGGTTT+TGG - chr8_1:20089504-20089523 Msa1184400:CDS 20.0%
!!! AGATTATAAGAAAGGTACTA+CGG + chr8_1:20085753-20085772 None:intergenic 25.0%
! CTAGAAGAAGATTATAAGAA+AGG + chr8_1:20085761-20085780 None:intergenic 25.0%
!! TTTTATGATATGATATTGGG+TGG - chr8_1:20086474-20086493 Msa1184400:intron 25.0%
!! TTTAAAACGTTGATAGACAA+AGG - chr8_1:20086582-20086601 Msa1184400:intron 25.0%
! ATTCAAAGTCTTCTTAGTAA+CGG - chr8_1:20086605-20086624 Msa1184400:intron 25.0%
! GACAATGCATAATTTATACA+AGG + chr8_1:20086953-20086972 None:intergenic 25.0%
! TTATTGTTCTGATACTAATG+CGG + chr8_1:20087071-20087090 None:intergenic 25.0%
!! CATATATCCACATTTTCTAT+TGG - chr8_1:20087691-20087710 Msa1184400:intron 25.0%
! AAATATCCCAATAGAAAATG+TGG + chr8_1:20087701-20087720 None:intergenic 25.0%
! AATTTCACTCGTTAGTTAAA+TGG - chr8_1:20087768-20087787 Msa1184400:intron 25.0%
!!! TTTTATTTTATTTGAGGGGA+GGG + chr8_1:20087877-20087896 None:intergenic 25.0%
!!! GGTGTTTTATTTTATTTGAG+GGG + chr8_1:20087881-20087900 None:intergenic 25.0%
!!! GAGGTGTTTTATTTTATTTG+AGG + chr8_1:20087883-20087902 None:intergenic 25.0%
! ATAAAATAAAACACCTCATG+TGG - chr8_1:20087886-20087905 Msa1184400:intron 25.0%
! GAAACTAAAAAAATGCGTAA+TGG - chr8_1:20087910-20087929 Msa1184400:intron 25.0%
! CTTGTTTGTAAAAGAAAGTT+TGG - chr8_1:20088036-20088055 Msa1184400:intron 25.0%
! ATGAAATTGTTGCATATAAC+TGG - chr8_1:20088119-20088138 Msa1184400:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG + chr8_1:20088334-20088353 None:intergenic 25.0%
!! TTTTTGCTTATAATAGTGAC+TGG - chr8_1:20088334-20088353 Msa1184400:intron 25.0%
!! TTTTGCTTATAATAGTGACT+GGG - chr8_1:20088335-20088354 Msa1184400:intron 25.0%
! CAATAAAAAGTTACTACTCT+TGG - chr8_1:20088619-20088638 Msa1184400:intron 25.0%
! TAGACAAACCCATAAAAATT+AGG - chr8_1:20088672-20088691 Msa1184400:intron 25.0%
!! CAAAGGTTCAATTAATTTTG+AGG - chr8_1:20088699-20088718 Msa1184400:intron 25.0%
!!! TCAATTAATTTTGAGGGAAA+AGG - chr8_1:20088706-20088725 Msa1184400:intron 25.0%
! TTCGTAATTAACAATAAAGG+GGG - chr8_1:20088778-20088797 Msa1184400:intron 25.0%
! ACATCTTACAATATTGGTAT+TGG + chr8_1:20089017-20089036 None:intergenic 25.0%
! TGATGAACATCTTACAATAT+TGG + chr8_1:20089023-20089042 None:intergenic 25.0%
! GATGAATGAATGATAGTTTA+TGG - chr8_1:20089340-20089359 Msa1184400:CDS 25.0%
! TTGTTTATTGACCAAATTGT+TGG - chr8_1:20089386-20089405 Msa1184400:CDS 25.0%
! TATCGAATAAACCAACAATT+TGG + chr8_1:20089400-20089419 None:intergenic 25.0%
! GCCAAAACCAATAAATATTA+AGG + chr8_1:20089508-20089527 None:intergenic 25.0%
!!! TAATATTTATTGGTTTTGGC+AGG - chr8_1:20089508-20089527 Msa1184400:CDS 25.0%
! TTTCTTACATGGATTGATGA+TGG + chr8_1:20085725-20085744 None:intergenic 30.0%
AAATGTGTCCAAGAATTTGT+TGG + chr8_1:20085837-20085856 None:intergenic 30.0%
AAAGATATTGCAAGCAGTAT+TGG - chr8_1:20085949-20085968 Msa1184400:CDS 30.0%
TATTAAGCAATCTAGTGTTG+AGG - chr8_1:20085996-20086015 Msa1184400:intron 30.0%
GAAGAAGAAGAATCTAATTC+AGG + chr8_1:20086082-20086101 None:intergenic 30.0%
CATGTTGAGAATTACATGAT+TGG - chr8_1:20086223-20086242 Msa1184400:intron 30.0%
TAAACAATCTTAGTACCACA+GGG + chr8_1:20086566-20086585 None:intergenic 30.0%
TTAAACAATCTTAGTACCAC+AGG + chr8_1:20086567-20086586 None:intergenic 30.0%
TTCAAAGTCTTCTTAGTAAC+GGG - chr8_1:20086606-20086625 Msa1184400:intron 30.0%
ATTACCTTAAAATAAGGCGT+TGG + chr8_1:20086698-20086717 None:intergenic 30.0%
ACAAAAACTGTTGTACCAAA+AGG + chr8_1:20086769-20086788 None:intergenic 30.0%
ATATTAGACATTGGATTTGG+AGG + chr8_1:20087207-20087226 None:intergenic 30.0%
GACATATTAGACATTGGATT+TGG + chr8_1:20087210-20087229 None:intergenic 30.0%
!! GTATATTTAATTGACTTGGC+CGG - chr8_1:20087504-20087523 Msa1184400:intron 30.0%
!! TTTGTTGGAAGTTGATATGT+AGG - chr8_1:20087527-20087546 Msa1184400:intron 30.0%
!! TTGTTGGAAGTTGATATGTA+GGG - chr8_1:20087528-20087547 Msa1184400:intron 30.0%
! AATGCTAATGCTTTTGACAA+TGG + chr8_1:20087580-20087599 None:intergenic 30.0%
! TCTAATTTTATTACGGCTAG+TGG - chr8_1:20087614-20087633 Msa1184400:intron 30.0%
ACTAACGAGTGAAATTAACA+AGG + chr8_1:20087764-20087783 None:intergenic 30.0%
TTTCACTAAGTGAAGGAATT+TGG + chr8_1:20087820-20087839 None:intergenic 30.0%
!!! TTTATTTTATTTGAGGGGAG+GGG + chr8_1:20087876-20087895 None:intergenic 30.0%
!!! GTTTTATTTTATTTGAGGGG+AGG + chr8_1:20087878-20087897 None:intergenic 30.0%
!!! TTTTTTAGTTTCTCCACATG+AGG + chr8_1:20087902-20087921 None:intergenic 30.0%
ACTAAAAAAATGCGTAATGG+AGG - chr8_1:20087913-20087932 Msa1184400:intron 30.0%
! TTTTCACGTCAAAAACAAGA+AGG - chr8_1:20087958-20087977 Msa1184400:intron 30.0%
TTTGCTTATAATAGTGACTG+GGG - chr8_1:20088336-20088355 Msa1184400:intron 30.0%
GTGCAATATTGCATATGTTA+AGG + chr8_1:20088648-20088667 None:intergenic 30.0%
ATTAATTGAGTACTCGTTCT+TGG - chr8_1:20088733-20088752 Msa1184400:intron 30.0%
TTAATTGAGTACTCGTTCTT+GGG - chr8_1:20088734-20088753 Msa1184400:intron 30.0%
AATTAACAATAAAGGGGGAA+AGG - chr8_1:20088783-20088802 Msa1184400:intron 30.0%
AAGACGAAAAGTAAATGAAG+AGG + chr8_1:20088976-20088995 None:intergenic 30.0%
!! TCTTCATTTACTTTTCGTCT+TGG - chr8_1:20088975-20088994 Msa1184400:CDS 30.0%
! AATGAAGTGTGCTGATAAAA+TGG - chr8_1:20089423-20089442 Msa1184400:CDS 30.0%
TTGATTAATGATGGTTGTGA+AGG - chr8_1:20089475-20089494 Msa1184400:CDS 30.0%
! TGAAATACAAGTCATCGATT+CGG - chr8_1:20089559-20089578 Msa1184400:CDS 30.0%
TATAGAATATGGGGAAATGT+TGG - chr8_1:20089603-20089622 Msa1184400:five_prime_UTR 30.0%
AATCAAGAAATGCAGTACAT+AGG + chr8_1:20089690-20089709 None:intergenic 30.0%
!! AATTTGTTGGCAATGGCATT+AGG + chr8_1:20085824-20085843 None:intergenic 35.0%
!! TTGATGATGATGAGAGGTAT+GGG - chr8_1:20086125-20086144 Msa1184400:intron 35.0%
GTTGAGAATTACATGATTGG+TGG - chr8_1:20086226-20086245 Msa1184400:intron 35.0%
TGAGTAGTAGAAGTAGAAAG+AGG + chr8_1:20086313-20086332 None:intergenic 35.0%
! TCATCAAGATGCTTGGATTT+TGG - chr8_1:20086330-20086349 Msa1184400:intron 35.0%
! TTCAAGGCATGGATTAATCA+AGG - chr8_1:20086525-20086544 Msa1184400:intron 35.0%
AACACTTGCCAAAATCACTA+CGG - chr8_1:20086641-20086660 Msa1184400:intron 35.0%
! CAAATTGTCCGTAGTGATTT+TGG + chr8_1:20086652-20086671 None:intergenic 35.0%
TTGCATAACCAAGGATTCAT+CGG - chr8_1:20086669-20086688 Msa1184400:intron 35.0%
! ATAATTAGCTAGCGACCTTT+TGG - chr8_1:20086751-20086770 Msa1184400:intron 35.0%
TTTGTGATATAAGCCTCCTT+AGG - chr8_1:20086784-20086803 Msa1184400:intron 35.0%
TTGTCATGAAAACGTTACCT+AGG + chr8_1:20086831-20086850 None:intergenic 35.0%
TATATAATTTAGCACGCGCT+AGG - chr8_1:20086853-20086872 Msa1184400:intron 35.0%
TTCCATAGAGACAAAACATG+TGG - chr8_1:20087095-20087114 Msa1184400:intron 35.0%
AAAACATGTGGCGTAATTGA+TGG - chr8_1:20087107-20087126 Msa1184400:intron 35.0%
TCCAATGTCTAATATGTCGA+CGG - chr8_1:20087212-20087231 Msa1184400:intron 35.0%
CATATCAACTTCCAACAAAC+CGG + chr8_1:20087526-20087545 None:intergenic 35.0%
ACAAGACAACATTGCATTGT+TGG + chr8_1:20087656-20087675 None:intergenic 35.0%
AATGTTGTCTTGTCATGAGA+AGG - chr8_1:20087663-20087682 Msa1184400:intron 35.0%
! TAAGTGAAGGAATTTGGAGT+TGG + chr8_1:20087814-20087833 None:intergenic 35.0%
AACTCTCTTTCACTAAGTGA+AGG + chr8_1:20087827-20087846 None:intergenic 35.0%
TACTACTCATATACTCTCTC+CGG - chr8_1:20088203-20088222 Msa1184400:intron 35.0%
TTGCTTATAATAGTGACTGG+GGG - chr8_1:20088337-20088356 Msa1184400:intron 35.0%
TATGTATGTTGTCCAAGTCA+AGG - chr8_1:20088914-20088933 Msa1184400:CDS 35.0%
! TTTGCATTGATACCTTGACT+TGG + chr8_1:20088929-20088948 None:intergenic 35.0%
CACAATTAAACATTGCGAGT+TGG + chr8_1:20089169-20089188 None:intergenic 35.0%
! GTGTGCTGATAAAATGGATT+AGG - chr8_1:20089429-20089448 Msa1184400:CDS 35.0%
GAAATACAAAGGACAAAGTG+CGG - chr8_1:20089536-20089555 Msa1184400:CDS 35.0%
! AAGAGGAAGGGTATAGAATA+TGG - chr8_1:20089592-20089611 Msa1184400:five_prime_UTR 35.0%
! AGAGGAAGGGTATAGAATAT+GGG - chr8_1:20089593-20089612 Msa1184400:five_prime_UTR 35.0%
AAATGCAGTACATAGGATTG+GGG + chr8_1:20089683-20089702 None:intergenic 35.0%
GAAATGCAGTACATAGGATT+GGG + chr8_1:20089684-20089703 None:intergenic 35.0%
AGAAATGCAGTACATAGGAT+TGG + chr8_1:20089685-20089704 None:intergenic 35.0%
TTTGTTGAGTACTACACAAG+TGG + chr8_1:20089730-20089749 None:intergenic 35.0%
!!! CTACGGTTGCTTTTCTTACA+TGG + chr8_1:20085736-20085755 None:intergenic 40.0%
TGCCATTGCCAACAAATTCT+TGG - chr8_1:20085826-20085845 Msa1184400:CDS 40.0%
!! GTCCAAGAATTTGTTGGCAA+TGG + chr8_1:20085831-20085850 None:intergenic 40.0%
! GATATTGCAAGCAGTATTGG+CGG - chr8_1:20085952-20085971 Msa1184400:CDS 40.0%
!! ATATTGCAAGCAGTATTGGC+GGG - chr8_1:20085953-20085972 Msa1184400:CDS 40.0%
!!! GTTTTTGCTTCTGAGGCTTT+TGG + chr8_1:20086036-20086055 None:intergenic 40.0%
! TGAAGCAGTTTTTGCTTCTG+AGG + chr8_1:20086043-20086062 None:intergenic 40.0%
TTCTTCTTCTTCTTCGTCGT+CGG - chr8_1:20086090-20086109 Msa1184400:intron 40.0%
!! GTTGATGATGATGAGAGGTA+TGG - chr8_1:20086124-20086143 Msa1184400:intron 40.0%
CAAACAATAATGTGCGTCCT+TGG - chr8_1:20086173-20086192 Msa1184400:intron 40.0%
ACACAATTTCATCCACGATG+AGG + chr8_1:20086273-20086292 None:intergenic 40.0%
CTACTACTCATCAAGATGCT+TGG - chr8_1:20086323-20086342 Msa1184400:intron 40.0%
TGAACATCGCCGAATCAAAT+GGG + chr8_1:20086509-20086528 None:intergenic 40.0%
TTGAACATCGCCGAATCAAA+TGG + chr8_1:20086510-20086529 None:intergenic 40.0%
!! ATTTGATTCGGCGATGTTCA+AGG - chr8_1:20086509-20086528 Msa1184400:intron 40.0%
!! TTAGTAACGGGTAGATACGA+AGG - chr8_1:20086618-20086637 Msa1184400:intron 40.0%
ACGGACAATTTGCATAACCA+AGG - chr8_1:20086660-20086679 Msa1184400:intron 40.0%
!!! GCTACCAACGCCTTATTTTA+AGG - chr8_1:20086691-20086710 Msa1184400:intron 40.0%
GATATAAGCCTCCTTAGGTT+AGG - chr8_1:20086789-20086808 Msa1184400:intron 40.0%
! TGAGCTTAACTCAGTTGGTA+AGG + chr8_1:20086975-20086994 None:intergenic 40.0%
TACCAACTGAGTTAAGCTCA+CGG - chr8_1:20086975-20086994 Msa1184400:intron 40.0%
CCAATGTCTAATATGTCGAC+GGG - chr8_1:20087213-20087232 Msa1184400:intron 40.0%
! TTATTACGGCTAGTGGATAC+AGG - chr8_1:20087621-20087640 Msa1184400:intron 40.0%
AGACAACATTGCATTGTTGG+AGG + chr8_1:20087653-20087672 None:intergenic 40.0%
GTAAGCAAGCATGCCAAATT+TGG + chr8_1:20088893-20088912 None:intergenic 40.0%
!! ATACAAGTCATCGATTCGGA+AGG - chr8_1:20089563-20089582 Msa1184400:exon 40.0%
! GAGGAAGGGTATAGAATATG+GGG - chr8_1:20089594-20089613 Msa1184400:five_prime_UTR 40.0%
!! TATTGCAAGCAGTATTGGCG+GGG - chr8_1:20085954-20085973 Msa1184400:CDS 45.0%
! GCGGGGTTAACGTTTTTGTT+GGG - chr8_1:20085971-20085990 Msa1184400:intron 45.0%
! CGGGGTTAACGTTTTTGTTG+GGG - chr8_1:20085972-20085991 Msa1184400:intron 45.0%
!! TGGTGGTTGATGATGATGAG+AGG - chr8_1:20086119-20086138 Msa1184400:intron 45.0%
CATGCTTGTAGCAACGAAGT+GGG + chr8_1:20086207-20086226 None:intergenic 45.0%
ACATGCTTGTAGCAACGAAG+TGG + chr8_1:20086208-20086227 None:intergenic 45.0%
! AGATGCTTGGATTTTGGACG+TGG - chr8_1:20086336-20086355 Msa1184400:intron 45.0%
CAGATGCGACCCATTTGATT+CGG - chr8_1:20086497-20086516 Msa1184400:intron 45.0%
TTAGTACCACAGGGTTTGCA+GGG + chr8_1:20086557-20086576 None:intergenic 45.0%
ATAAGCCTCCTTAGGTTAGG+TGG - chr8_1:20086792-20086811 Msa1184400:intron 45.0%
ACTATCCACCTAACCTAAGG+AGG + chr8_1:20086800-20086819 None:intergenic 45.0%
GTCACTATCCACCTAACCTA+AGG + chr8_1:20086803-20086822 None:intergenic 45.0%
ACCAACTGAGTTAAGCTCAC+GGG - chr8_1:20086976-20086995 Msa1184400:intron 45.0%
! CGCCACATGTTTTGTCTCTA+TGG + chr8_1:20087100-20087119 None:intergenic 45.0%
AGACATTGGATTTGGAGGAG+TGG + chr8_1:20087202-20087221 None:intergenic 45.0%
CCCGTCGACATATTAGACAT+TGG + chr8_1:20087216-20087235 None:intergenic 45.0%
!! AATTGACTTGGCCGGTTTGT+TGG - chr8_1:20087512-20087531 Msa1184400:intron 45.0%
! TGCGTAATGGAGGAACTTCA+TGG - chr8_1:20087923-20087942 Msa1184400:intron 45.0%
GCTGCTGACAATGCCAAATT+TGG - chr8_1:20088877-20088896 Msa1184400:CDS 45.0%
! GGCAGGAGTGTGAAATACAA+AGG - chr8_1:20089525-20089544 Msa1184400:CDS 45.0%
GATTCGGAAGGAGATTGAAG+AGG - chr8_1:20089575-20089594 Msa1184400:exon 45.0%
GGAAGGAGATTGAAGAGGAA+GGG - chr8_1:20089580-20089599 Msa1184400:five_prime_UTR 45.0%
!!! TTTTATTTATTTTAAAGATT+TGG + chr8_1:20087463-20087482 None:intergenic 5.0%
! GGCGGGGTTAACGTTTTTGT+TGG - chr8_1:20085970-20085989 Msa1184400:intron 50.0%
TTCTTCGTCGTCGGAGATGA+TGG - chr8_1:20086099-20086118 Msa1184400:intron 50.0%
GCATGACATGAACCTCATCG+TGG - chr8_1:20086258-20086277 Msa1184400:intron 50.0%
ATTCGGCGATGTTCAAGGCA+TGG - chr8_1:20086514-20086533 Msa1184400:intron 50.0%
CTTAGTACCACAGGGTTTGC+AGG + chr8_1:20086558-20086577 None:intergenic 50.0%
!! GTTGGTAGCCGATGAATCCT+TGG + chr8_1:20086680-20086699 None:intergenic 50.0%
GTGGATAGTGACTGCTACCT+AGG - chr8_1:20086811-20086830 Msa1184400:intron 50.0%
CCAACTGAGTTAAGCTCACG+GGG - chr8_1:20086977-20086996 Msa1184400:intron 50.0%
AGTTAAGCTCACGGGGACAA+TGG - chr8_1:20086984-20087003 Msa1184400:intron 50.0%
GTTAAGCTCACGGGGACAAT+GGG - chr8_1:20086985-20087004 Msa1184400:intron 50.0%
! GGGGAAAGGCACAATAGACT+TGG - chr8_1:20088797-20088816 Msa1184400:intron 50.0%
CGGAAGGAGATTGAAGAGGA+AGG - chr8_1:20089579-20089598 Msa1184400:five_prime_UTR 50.0%
!! ACCAGTGGACTGATCTCCAA+GGG - chr8_1:20089628-20089647 Msa1184400:five_prime_UTR 50.0%
!! TGATCTCCAAGGGGAGTGTT+TGG - chr8_1:20089638-20089657 Msa1184400:five_prime_UTR 50.0%
!! GATCTCCAAGGGGAGTGTTT+GGG - chr8_1:20089639-20089658 Msa1184400:five_prime_UTR 50.0%
! TTCGTCGTCGGAGATGATGG+TGG - chr8_1:20086102-20086121 Msa1184400:intron 55.0%
! ATGAGAGGTATGGGCTGAGC+TGG - chr8_1:20086134-20086153 Msa1184400:intron 55.0%
CGAAGTGGGACTGTCTTCCA+AGG + chr8_1:20086193-20086212 None:intergenic 55.0%
AATGTGCCCTGCAAACCCTG+TGG - chr8_1:20086548-20086567 Msa1184400:intron 55.0%
CCCCGTGAGCTTAACTCAGT+TGG + chr8_1:20086980-20086999 None:intergenic 55.0%
GGGGAAATGTTGGAGACCAG+TGG - chr8_1:20089613-20089632 Msa1184400:five_prime_UTR 55.0%
!! GACCAGTGGACTGATCTCCA+AGG - chr8_1:20089627-20089646 Msa1184400:five_prime_UTR 55.0%
!! CCAGTGGACTGATCTCCAAG+GGG - chr8_1:20089629-20089648 Msa1184400:five_prime_UTR 55.0%
GATTGCCCAAACACTCCCCT+TGG + chr8_1:20089647-20089666 None:intergenic 55.0%
! AGAGGTATGGGCTGAGCTGG+AGG - chr8_1:20086137-20086156 Msa1184400:intron 60.0%
! GTATGGGCTGAGCTGGAGGT+TGG - chr8_1:20086141-20086160 Msa1184400:intron 60.0%
GCTGAGCTGGAGGTTGGCAA+TGG - chr8_1:20086147-20086166 Msa1184400:intron 60.0%
CCCCTTGGAGATCAGTCCAC+TGG + chr8_1:20089632-20089651 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 20085721 20089792 20085721 ID=Msa1184400;Name=Msa1184400
chr8_1 mRNA 20085721 20089792 20085721 ID=Msa1184400-mRNA-1;Parent=Msa1184400;Name=Msa1184400-mRNA-1;_AED=0.11;_eAED=0.11;_QI=214|1|1|1|1|1|3|85|287
chr8_1 exon 20085721 20085983 20085721 ID=Msa1184400-mRNA-1:exon:7787;Parent=Msa1184400-mRNA-1
chr8_1 exon 20088801 20089013 20088801 ID=Msa1184400-mRNA-1:exon:7786;Parent=Msa1184400-mRNA-1
chr8_1 exon 20089106 20089792 20089106 ID=Msa1184400-mRNA-1:exon:7785;Parent=Msa1184400-mRNA-1
chr8_1 five_prime_UTR 20089579 20089792 20089579 ID=Msa1184400-mRNA-1:five_prime_utr;Parent=Msa1184400-mRNA-1
chr8_1 CDS 20089106 20089578 20089106 ID=Msa1184400-mRNA-1:cds;Parent=Msa1184400-mRNA-1
chr8_1 CDS 20088801 20089013 20088801 ID=Msa1184400-mRNA-1:cds;Parent=Msa1184400-mRNA-1
chr8_1 CDS 20085806 20085983 20085806 ID=Msa1184400-mRNA-1:cds;Parent=Msa1184400-mRNA-1
chr8_1 three_prime_UTR 20085721 20085805 20085721 ID=Msa1184400-mRNA-1:three_prime_utr;Parent=Msa1184400-mRNA-1
Gene Sequence

>Msa1184400

ATGATGCTAAAGATAAAGATATTGCAAGCAGTATTGGCGGGGTTAACGTTTTTGTTGGGGTTATTAAGCAATCTAGTGTTGAGGCTGCAGAAACAGAGCCAAAAGCCTCAGAAGCAAAAACTGCTTCAGATGATGAATATCAAACCTGAATTAGATTCTTCTTCTTCTTCGTCGTCGGAGATGATGGTGGTTGATGATGATGAGAGGTATGGGCTGAGCTGGAGGTTGGCAATGGAGACAAACAATAATGTGCGTCCTTGGAAGACAGTCCCACTTCGTTGCTACAAGCATGTTGAGAATTACATGATTGGTGGACAATACGAGCATGACATGAACCTCATCGTGGATGAAATTGTGTTTTATGCATCTCAGATTCCTCTTTCTACTTCTACTACTCATCAAGATGCTTGGATTTTGGACGTGGACGACACTTGTATCTCTAATATTCCTTATTATAAAGCTAAGAGATTTGGATGCGACCCATTTGATTCGGCGATGTTCAAGGCATGGATTAATCAAGGAATGTGCCCTGCAAACCCTGTGGTACTAAGATTGTTTAAAACGTTGATAGACAAAGGATTCAAAGTCTTCTTAGTAACGGGTAGATACGAAGGAACACTTGCCAAAATCACTACGGACAATTTGCATAACCAAGGATTCATCGGCTACCAACGCCTTATTTTAAGGAGTGTGAAATACAAAGGACAAAGTGCGGTGAAATACAAGTCATCGATTCGGAAGGAGATTGAAGAGGAAGGGTATAGAATATGGGGAAATGTTGGAGACCAGTGGACTGATCTCCAAGGGGAGTGTTTGGGCAATCGTACTTTCAAGATCCCCAATCCTATGTACTGCATTTCTTGA
Protein sequence

>Msa1184400

MMLKIKILQAVLAGLTFLLGLLSNLVLRLQKQSQKPQKQKLLQMMNIKPELDSSSSSSSEMMVVDDDERYGLSWRLAMETNNNVRPWKTVPLRCYKHVENYMIGGQYEHDMNLIVDEIVFYASQIPLSTSTTHQDAWILDVDDTCISNIPYYKAKRFGCDPFDSAMFKAWINQGMCPANPVVLRLFKTLIDKGFKVFLVTGRYEGTLAKITTDNLHNQGFIGYQRLILRSVKYKGQSAVKYKSSIRKEIEEEGYRIWGNVGDQWTDLQGECLGNRTFKIPNPMYCIS*