AlfalfaGEDB | Msa1236320

M. sativa cultivar zhongmu-4 / Msa1236320

KEGG: K09338
GO: GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009891 GO:0009893 GO:0010033 GO:0010035 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033993 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1901363 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF00046.32

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236320	AFV30231.1	100.000	247	0	0	1	247	1	247	0.0	507

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236320	sp\|P46897\|ATHB7_ARATH	49.012	253	108	6	15	247	7	258	1.49e-63	202

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa1236320	TF	HB-HD-ZIP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0256390	Msa1236320	0.802808	4.705170e-49	-8.615850e-47
Msa1176120	Msa1236320	0.985115	1.424136e-162	-8.615850e-47
Msa1236320	Msa1277440	0.942529	6.114899e-102	-8.615850e-47
Msa1236320	Msa1316250	0.993267	1.445978e-198	-8.615850e-47
Msa1236320	Msa1359840	0.807328	5.332264e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236320	MtrunA17_Chr8g0346251	96.371	248	8	1	1	247	1	248	8.48e-178	488
Msa1236320	MtrunA17_Chr3g0127511	43.062	209	113	3	42	247	40	245	6.22e-48	158
Msa1236320	MtrunA17_Chr6g0450991	53.125	96	45	0	42	137	69	164	1.07e-29	112
Msa1236320	MtrunA17_Chr8g0381741	48.148	108	56	0	42	149	56	163	3.45e-29	111
Msa1236320	MtrunA17_Chr6g0453021	50.000	112	47	2	21	132	86	188	1.37e-26	103
Msa1236320	MtrunA17_Chr3g0123691	38.547	179	97	6	20	194	44	213	3.23e-26	103
Msa1236320	MtrunA17_Chr8g0362151	40.945	127	70	1	8	134	50	171	5.23e-26	102
Msa1236320	MtrunA17_Chr5g0415941	48.571	105	53	1	42	145	89	193	7.15e-26	103
Msa1236320	MtrunA17_Chr8g0362751	37.222	180	90	6	42	204	91	264	2.84e-25	100
Msa1236320	MtrunA17_Chr4g0060031	42.478	113	62	1	42	154	97	206	6.54e-25	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236320	AT2G46680.1	49.012	253	108	6	15	247	7	258	1.51e-64	202
Msa1236320	AT3G61890.1	55.238	210	82	5	43	247	33	235	3.89e-63	197
Msa1236320	AT2G46680.2	51.754	228	87	7	40	247	32	256	3.80e-61	193
Msa1236320	AT3G01470.1	44.531	128	70	1	42	168	70	197	5.78e-31	115
Msa1236320	AT2G22430.1	52.747	91	43	0	42	132	64	154	1.71e-28	110
Msa1236320	AT4G40060.1	50.549	91	45	0	42	132	61	151	3.81e-28	108
Msa1236320	AT5G65310.2	48.113	106	49	1	27	132	47	146	1.75e-27	107
Msa1236320	AT5G65310.1	48.113	106	49	1	27	132	65	164	2.42e-27	107
Msa1236320	AT5G15150.1	39.041	146	78	3	18	162	96	231	5.03e-26	103
Msa1236320	AT1G69780.1	45.946	111	55	2	22	132	72	177	6.76e-25	100

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Find 53 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTTGAATCTATGAAGA+AGG	0.456244	8_2:+15011600	Msa1236320:CDS
AAATGTGATAATAGTCATTT+TGG	0.188003	8_2:+15012821	Msa1236320:three_prime_UTR
AACATCATATGAATTAGGTG+AGG	0.651625	8_2:-15011247	None:intergenic
AACCATATAGCAACTTGTCT+TGG	0.480304	8_2:-15011493	None:intergenic
AACTTTGGTTATGGCTTAAA+AGG	0.223392	8_2:-15012728	None:intergenic
AAGACAAACATTACTAATAC+AGG	0.463639	8_2:+15011624	Msa1236320:CDS
AAGTTGTCAGGGGAAAATCA+AGG	0.603486	8_2:+15012427	Msa1236320:three_prime_UTR
AAGTTGTGATTATCAATGGT+GGG	0.604345	8_2:+15012310	Msa1236320:CDS
AATCAAGTTGTGATTATCAA+TGG	0.520398	8_2:+15012306	Msa1236320:CDS
ACAGAGGAGGCAAAATTATG+TGG	0.582377	8_2:+15012352	Msa1236320:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACATCATATGAATTAGGTG+AGG	-	chr8_2:15011250-15011269	None:intergenic	30.0%
TTCAAAACAAAAGAGCTAGA+TGG	+	chr8_2:15011515-15011534	Msa1236320:CDS	30.0%
TGTTCTGATCTTTCTATTGA+AGG	-	chr8_2:15012128-15012147	None:intergenic	30.0%
AGCATAAAGGTTGAATACTT+TGG	+	chr8_2:15012179-15012198	Msa1236320:CDS	30.0%
AAGTTGTGATTATCAATGGT+GGG	+	chr8_2:15012310-15012329	Msa1236320:CDS	30.0%
TATGGCTTAAAAGGTCTATT+TGG	-	chr8_2:15012722-15012741	None:intergenic	30.0%
AACTTTGGTTATGGCTTAAA+AGG	-	chr8_2:15012731-15012750	None:intergenic	30.0%
AACATCATATGAATTAGGTG+AGG	-	chr8_2:15011250-15011269	None:intergenic	30.0%
TTCAAAACAAAAGAGCTAGA+TGG	+	chr8_2:15011515-15011534	Msa1236320:CDS	30.0%
TGTTCTGATCTTTCTATTGA+AGG	-	chr8_2:15012128-15012147	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	15011139	15012870	15011139	ID=Msa1236320;Name=Msa1236320
chr8_2	mRNA	15011139	15012870	15011139	ID=Msa1236320-mRNA-1;Parent=Msa1236320;Name=Msa1236320-mRNA-1;_AED=0.00;_eAED=0.00;_QI=123\|1\|1\|1\|1\|1\|2\|524\|247
chr8_2	exon	15011139	15011645	15011139	ID=Msa1236320-mRNA-1:exon:5754;Parent=Msa1236320-mRNA-1
chr8_2	exon	15011987	15012870	15011987	ID=Msa1236320-mRNA-1:exon:5755;Parent=Msa1236320-mRNA-1
chr8_2	five_prime_UTR	15011139	15011261	15011139	ID=Msa1236320-mRNA-1:five_prime_utr;Parent=Msa1236320-mRNA-1
chr8_2	CDS	15011262	15011645	15011262	ID=Msa1236320-mRNA-1:cds;Parent=Msa1236320-mRNA-1
chr8_2	CDS	15011987	15012346	15011987	ID=Msa1236320-mRNA-1:cds;Parent=Msa1236320-mRNA-1
chr8_2	three_prime_UTR	15012347	15012870	15012347	ID=Msa1236320-mRNA-1:three_prime_utr;Parent=Msa1236320-mRNA-1

Gene Sequence

>Msa1236320

ATGATGTTTGAATTAGACAATATGGAATATAGTCAATATAGTTATTCAGCAGAAGCAGGAGAAGAAGAAACATACACAACTAGTAGCACATCTTCCATGAGAAAGAAGAAAAACAAGAATACAAAGAGGTTTAGTGATGAACAAATCAAATCATTGGAAACTATGTTTGAGACTGAGACAAGACTTGAACCAAGAAAGAAGTTGCAATTAGCTAGAGAGCTTGGATTGCAGCCAAGACAAGTTGCTATATGGTTTCAAAACAAAAGAGCTAGATGGAAATCAAAGCAACTTGAAAGAGAATACAACAAACTTCAAAATAGTTACAATAATTTGGCTTCAAAGTTTGAATCTATGAAGAAGGAAAGACAAACATTACTAATACAGTTGCAGAAGCTGAATGATCTAATACAAAAGCCAATAGAGCAAAGTCAGAGTAGTTCACAAGTTAAAGAAGCAAACAGCATGGAAAGTGCAGCAGAAAATGGAGGAAAAATCAAATGTGAGGCTGAGGTGAAACCAAGTCCTTCAATAGAAAGATCAGAACATGTACTTGATGTTCTATCAGATGATGACACAAGCATAAAGGTTGAATACTTTGGTTTAGAAGATGAAACTGGTCTTATGAATTTTGCTGAACATGCTGATGGTTCTTTAACATCACCAGAAGATTGGAGTGCTTTTGAATCAAATGATTTGTTAGGTCAATCAAGTTGTGATTATCAATGGTGGGACTTTTGGTCTTGA

Protein sequence

>Msa1236320

MMFELDNMEYSQYSYSAEAGEEETYTTSSTSSMRKKKNKNTKRFSDEQIKSLETMFETETRLEPRKKLQLARELGLQPRQVAIWFQNKRARWKSKQLEREYNKLQNSYNNLASKFESMKKERQTLLIQLQKLNDLIQKPIEQSQSSSQVKEANSMESAAENGGKIKCEAEVKPSPSIERSEHVLDVLSDDDTSIKVEYFGLEDETGLMNFAEHADGSLTSPEDWSAFESNDLLGQSSCDYQWWDFWS*