AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1236320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1236320 MtrunA17_Chr8g0346251 96.371 248 8 1 1 247 1 248 8.48e-178 488
Msa1236320 MtrunA17_Chr3g0127511 43.062 209 113 3 42 247 40 245 6.22e-48 158
Msa1236320 MtrunA17_Chr6g0450991 53.125 96 45 0 42 137 69 164 1.07e-29 112
Msa1236320 MtrunA17_Chr8g0381741 48.148 108 56 0 42 149 56 163 3.45e-29 111
Msa1236320 MtrunA17_Chr6g0453021 50.000 112 47 2 21 132 86 188 1.37e-26 103
Msa1236320 MtrunA17_Chr3g0123691 38.547 179 97 6 20 194 44 213 3.23e-26 103
Msa1236320 MtrunA17_Chr8g0362151 40.945 127 70 1 8 134 50 171 5.23e-26 102
Msa1236320 MtrunA17_Chr5g0415941 48.571 105 53 1 42 145 89 193 7.15e-26 103
Msa1236320 MtrunA17_Chr8g0362751 37.222 180 90 6 42 204 91 264 2.84e-25 100
Msa1236320 MtrunA17_Chr4g0060031 42.478 113 62 1 42 154 97 206 6.54e-25 100
Msa1236320 MtrunA17_Chr5g0416391 40.984 122 72 0 13 134 50 171 7.60e-25 99.8
Msa1236320 MtrunA17_Chr1g0179581 52.381 84 33 1 42 125 30 106 3.17e-24 93.6
Msa1236320 MtrunA17_Chr3g0119291 47.253 91 48 0 42 132 61 151 1.22e-23 95.9
Msa1236320 MtrunA17_Chr7g0265521 48.352 91 40 1 42 132 65 148 6.49e-22 90.9
Msa1236320 MtrunA17_Chr7g0217111 50.000 90 38 1 43 132 83 165 3.14e-21 89.7
Msa1236320 MtrunA17_Chr5g0403701 51.852 81 34 1 28 108 46 121 3.18e-18 80.1
Msa1236320 MtrunA17_Chr5g0403691 51.852 81 34 1 28 108 46 121 1.07e-17 79.0
Msa1236320 MtrunA17_Chr4g0055851 44.578 83 46 0 43 125 123 205 2.64e-14 70.9
Msa1236320 MtrunA17_Chr7g0258831 40.741 108 59 1 18 125 143 245 4.63e-14 70.1
Msa1236320 MtrunA17_Chr4g0071411 36.975 119 67 2 43 160 91 202 6.14e-14 68.9
Msa1236320 MtrunA17_Chr1g0175651 36.667 120 72 1 6 125 124 239 9.88e-13 66.6
Msa1236320 MtrunA17_Chr5g0400781 35.200 125 70 3 43 163 126 243 1.68e-12 65.5
Msa1236320 MtrunA17_Chr5g0399491 42.169 83 48 0 43 125 139 221 7.33e-12 63.9
Msa1236320 MtrunA17_Chr2g0308611 41.111 90 50 1 39 125 196 285 1.04e-11 63.9
Msa1236320 MtrunA17_Chr4g0054421 42.169 83 48 0 43 125 130 212 1.77e-11 62.8
Msa1236320 MtrunA17_Chr3g0135331 31.707 123 80 2 43 162 148 269 2.12e-11 62.8
Msa1236320 MtrunA17_Chr8g0335131 42.169 83 48 0 43 125 149 231 3.54e-11 62.0
Msa1236320 MtrunA17_Chr1g0151971 40.964 83 49 0 43 125 133 215 5.72e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1236320 AT2G46680.1 49.012 253 108 6 15 247 7 258 1.51e-64 202
Msa1236320 AT3G61890.1 55.238 210 82 5 43 247 33 235 3.89e-63 197
Msa1236320 AT2G46680.2 51.754 228 87 7 40 247 32 256 3.80e-61 193
Msa1236320 AT3G01470.1 44.531 128 70 1 42 168 70 197 5.78e-31 115
Msa1236320 AT2G22430.1 52.747 91 43 0 42 132 64 154 1.71e-28 110
Msa1236320 AT4G40060.1 50.549 91 45 0 42 132 61 151 3.81e-28 108
Msa1236320 AT5G65310.2 48.113 106 49 1 27 132 47 146 1.75e-27 107
Msa1236320 AT5G65310.1 48.113 106 49 1 27 132 65 164 2.42e-27 107
Msa1236320 AT5G15150.1 39.041 146 78 3 18 162 96 231 5.03e-26 103
Msa1236320 AT1G69780.1 45.946 111 55 2 22 132 72 177 6.76e-25 100
Msa1236320 AT3G01220.1 45.536 112 51 2 21 132 71 172 1.62e-24 99.4
Msa1236320 AT4G36740.2 57.746 71 30 0 42 112 58 128 2.35e-22 92.0
Msa1236320 AT4G36740.1 57.746 71 30 0 42 112 57 127 2.45e-22 91.7
Msa1236320 AT1G26960.1 47.788 113 46 3 20 132 57 156 1.53e-21 90.5
Msa1236320 AT2G18550.1 42.017 119 66 1 42 157 63 181 2.66e-21 89.4
Msa1236320 AT5G03790.1 44.792 96 46 1 42 137 79 167 3.85e-21 89.0
Msa1236320 AT1G27045.4 45.098 102 56 0 42 143 70 171 1.58e-20 87.8
Msa1236320 AT5G03790.2 45.263 95 52 0 42 136 79 173 1.93e-20 86.3
Msa1236320 AT5G66700.1 53.521 71 33 0 42 112 73 143 3.45e-20 86.3
Msa1236320 AT1G27045.5 48.810 84 43 0 42 125 27 110 3.17e-19 82.8
Msa1236320 AT1G27045.3 48.810 84 43 0 42 125 27 110 3.17e-19 82.8
Msa1236320 AT1G27045.2 48.810 84 43 0 42 125 19 102 3.24e-19 82.4
Msa1236320 AT1G27045.1 48.810 84 43 0 42 125 70 153 3.94e-19 83.6
Msa1236320 AT2G36610.1 41.176 102 48 2 39 132 70 167 5.34e-18 79.3
Msa1236320 AT5G53980.1 41.071 112 58 2 31 142 3 106 1.10e-16 75.1
Msa1236320 AT4G37790.1 40.964 83 49 0 43 125 129 211 1.49e-11 63.2
Msa1236320 AT2G22800.1 42.169 83 48 0 43 125 116 198 1.71e-11 63.2
Msa1236320 AT2G44910.1 41.772 79 46 0 43 121 166 244 1.98e-11 63.2
Msa1236320 AT3G60390.1 41.772 79 46 0 43 121 165 243 2.01e-11 63.2
Msa1236320 AT5G06710.1 39.759 83 50 0 43 125 193 275 2.94e-11 62.8
Msa1236320 AT2G01430.1 35.455 110 67 1 16 125 119 224 3.94e-11 62.0
Msa1236320 AT4G17460.1 41.772 79 46 0 43 121 138 216 5.12e-11 61.6
Msa1236320 AT4G16780.1 41.772 79 46 0 43 121 132 210 6.42e-11 61.6

Find 53 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAAATAGTTACAATAATT+TGG 0.124744 8_2:+15011573 Msa1236320:CDS
AGGCTATTCTGGGGAGTTTA+TGG 0.159349 8_2:+15012616 Msa1236320:three_prime_UTR
AAATGTGATAATAGTCATTT+TGG 0.188003 8_2:+15012821 Msa1236320:three_prime_UTR
TATGGCTTAAAAGGTCTATT+TGG 0.197309 8_2:-15012719 None:intergenic
CTACTCTGACTTTGCTCTAT+TGG 0.221851 8_2:-15012017 None:intergenic
GCTAATTGCAACTTCTTTCT+TGG 0.222692 8_2:-15011451 None:intergenic
AACTTTGGTTATGGCTTAAA+AGG 0.223392 8_2:-15012728 None:intergenic
TGGCTACTTGTTACACATTC+AGG 0.269451 8_2:-15012846 None:intergenic
ATTCAAACATCATATGAATT+AGG 0.288055 8_2:-15011252 None:intergenic
ATTATCAATGGTGGGACTTT+TGG 0.297638 8_2:+15012318 Msa1236320:CDS
CTCTGCGGTTTGATCATGTT+TGG 0.309960 8_2:+15012505 Msa1236320:three_prime_UTR
TTTGAATCAAATGATTTGTT+AGG 0.319546 8_2:+15012281 Msa1236320:CDS
AGCCAAGACAAGTTGCTATA+TGG 0.331941 8_2:+15011491 Msa1236320:CDS
CATTAAAAGAACTTTGGTTA+TGG 0.336793 8_2:-15012737 None:intergenic
GATGTTTGAATTAGACAATA+TGG 0.344720 8_2:+15011264 Msa1236320:CDS
CAACATAATCATTAGTTTGT+AGG 0.346723 8_2:+15012469 Msa1236320:three_prime_UTR
TCAAAAGCACTCCAATCTTC+TGG 0.368233 8_2:-15012263 None:intergenic
TGTTCTGATCTTTCTATTGA+AGG 0.372040 8_2:-15012125 None:intergenic
ATGGAAAGTGCAGCAGAAAA+TGG 0.377944 8_2:+15012065 Msa1236320:CDS
CTTTAACATCACCAGAAGAT+TGG 0.390865 8_2:+15012252 Msa1236320:CDS
TGATGAACAAATCAAATCAT+TGG 0.409448 8_2:+15011396 Msa1236320:CDS
TTTGGTTTAGAAGATGAAAC+TGG 0.409611 8_2:+15012197 Msa1236320:CDS
AGCATAAAGGTTGAATACTT+TGG 0.411821 8_2:+15012179 Msa1236320:CDS
TGTCAACATTAAAAGAACTT+TGG 0.430634 8_2:-15012743 None:intergenic
TTCAAAACAAAAGAGCTAGA+TGG 0.440130 8_2:+15011515 Msa1236320:CDS
TTGCAATTAGCTAGAGAGCT+TGG 0.453561 8_2:+15011463 Msa1236320:CDS
AAAGTTTGAATCTATGAAGA+AGG 0.456244 8_2:+15011600 Msa1236320:CDS
AGATGATGACACAAGCATAA+AGG 0.461800 8_2:+15012166 Msa1236320:CDS
AAGACAAACATTACTAATAC+AGG 0.463639 8_2:+15011624 Msa1236320:CDS
AACCATATAGCAACTTGTCT+TGG 0.480304 8_2:-15011493 None:intergenic
GATCTTTCTATTGAAGGACT+TGG 0.483251 8_2:-15012119 None:intergenic
TATAGTTATTCAGCAGAAGC+AGG 0.509566 8_2:+15011298 Msa1236320:CDS
AATCAAGTTGTGATTATCAA+TGG 0.520398 8_2:+15012306 Msa1236320:CDS
TAGTAGACTACATTAACTAT+TGG 0.524007 8_2:-15012770 None:intergenic
TTATTTGAGTTCTTATCATG+AGG 0.553646 8_2:-15011145 None:intergenic
CAAGTTGTGATTATCAATGG+TGG 0.557189 8_2:+15012309 Msa1236320:CDS
TATTTGAGTTCTTATCATGA+GGG 0.572779 8_2:-15011144 None:intergenic
AGTTTATGGAAGTAAGCTGA+TGG 0.580260 8_2:+15012630 Msa1236320:three_prime_UTR
ACAGAGGAGGCAAAATTATG+TGG 0.582377 8_2:+15012352 Msa1236320:three_prime_UTR
AAGTTGTCAGGGGAAAATCA+AGG 0.603486 8_2:+15012427 Msa1236320:three_prime_UTR
AAGTTGTGATTATCAATGGT+GGG 0.604345 8_2:+15012310 Msa1236320:CDS
AGTTGTCAGGGGAAAATCAA+GGG 0.618503 8_2:+15012428 Msa1236320:three_prime_UTR
GGTCTTGAATGAAACAGAGG+AGG 0.619886 8_2:+15012339 Msa1236320:exon
TTTGGTCTTGAATGAAACAG+AGG 0.622140 8_2:+15012336 Msa1236320:exon
ATGCATGTATCTATATATGT+TGG 0.622370 8_2:-15011175 None:intergenic
ATTTGAGTTCTTATCATGAG+GGG 0.634012 8_2:-15011143 None:intergenic
AGTTAAAGAAGCAAACAGCA+TGG 0.635070 8_2:+15012046 Msa1236320:CDS
GTTTATGGAAGTAAGCTGAT+GGG 0.635413 8_2:+15012631 Msa1236320:three_prime_UTR
TTTGTTGAAGAAGTTGTCAG+GGG 0.642443 8_2:+15012417 Msa1236320:three_prime_UTR
AACATCATATGAATTAGGTG+AGG 0.651625 8_2:-15011247 None:intergenic
GAGGAGGCAAAATTATGTGG+AGG 0.659550 8_2:+15012355 Msa1236320:three_prime_UTR
GAAAGTGCAGCAGAAAATGG+AGG 0.672573 8_2:+15012068 Msa1236320:CDS
GATACATGCATATCATCACA+AGG 0.717524 8_2:+15011187 Msa1236320:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAAATAGTTACAATAATT+TGG + chr8_2:15011573-15011592 Msa1236320:CDS 15.0%
!! TGCGAAATTATTATTAATAA+CGG + chr8_2:15011773-15011792 Msa1236320:intron 15.0%
!! TCAAAATAGTTACAATAATT+TGG + chr8_2:15011573-15011592 Msa1236320:CDS 15.0%
!! TGCGAAATTATTATTAATAA+CGG + chr8_2:15011773-15011792 Msa1236320:intron 15.0%
!! ATTCAAACATCATATGAATT+AGG - chr8_2:15011255-15011274 None:intergenic 20.0%
!!! ATAAATTTGTTTGCTTAGAA+TGG + chr8_2:15011681-15011700 Msa1236320:intron 20.0%
!! GAAATTATTATTAATAACGG+TGG + chr8_2:15011776-15011795 Msa1236320:intron 20.0%
!! TAATCATATAGTAATTTGAC+TGG + chr8_2:15011948-15011967 Msa1236320:intron 20.0%
!!! TTTGAATCAAATGATTTGTT+AGG + chr8_2:15012281-15012300 Msa1236320:CDS 20.0%
!!! AAGTTATAAGTTGTTTTCAA+AGG + chr8_2:15012596-15012615 Msa1236320:three_prime_UTR 20.0%
!!! AAATGTGATAATAGTCATTT+TGG + chr8_2:15012821-15012840 Msa1236320:three_prime_UTR 20.0%
!! ATTCAAACATCATATGAATT+AGG - chr8_2:15011255-15011274 None:intergenic 20.0%
!!! ATAAATTTGTTTGCTTAGAA+TGG + chr8_2:15011681-15011700 Msa1236320:intron 20.0%
!! GAAATTATTATTAATAACGG+TGG + chr8_2:15011776-15011795 Msa1236320:intron 20.0%
!! TAATCATATAGTAATTTGAC+TGG + chr8_2:15011948-15011967 Msa1236320:intron 20.0%
!!! TTTGAATCAAATGATTTGTT+AGG + chr8_2:15012281-15012300 Msa1236320:CDS 20.0%
!!! AAGTTATAAGTTGTTTTCAA+AGG + chr8_2:15012596-15012615 Msa1236320:three_prime_UTR 20.0%
!!! AAATGTGATAATAGTCATTT+TGG + chr8_2:15012821-15012840 Msa1236320:three_prime_UTR 20.0%
!! TATTTGAGTTCTTATCATGA+GGG - chr8_2:15011147-15011166 None:intergenic 25.0%
!! TTATTTGAGTTCTTATCATG+AGG - chr8_2:15011148-15011167 None:intergenic 25.0%
! ATGCATGTATCTATATATGT+TGG - chr8_2:15011178-15011197 None:intergenic 25.0%
! GATGTTTGAATTAGACAATA+TGG + chr8_2:15011264-15011283 Msa1236320:CDS 25.0%
! AGAAAAACAAGAATACAAAG+AGG + chr8_2:15011368-15011387 Msa1236320:CDS 25.0%
! TGATGAACAAATCAAATCAT+TGG + chr8_2:15011396-15011415 Msa1236320:CDS 25.0%
! AAAGTTTGAATCTATGAAGA+AGG + chr8_2:15011600-15011619 Msa1236320:CDS 25.0%
! AAGACAAACATTACTAATAC+AGG + chr8_2:15011624-15011643 Msa1236320:CDS 25.0%
! ATCTTGTCAACATTTCAAAA+TGG - chr8_2:15011749-15011768 None:intergenic 25.0%
!! AATCAAGTTGTGATTATCAA+TGG + chr8_2:15012306-15012325 Msa1236320:CDS 25.0%
!!! CAACATAATCATTAGTTTGT+AGG + chr8_2:15012469-15012488 Msa1236320:three_prime_UTR 25.0%
! CATTAAAAGAACTTTGGTTA+TGG - chr8_2:15012740-15012759 None:intergenic 25.0%
! TGTCAACATTAAAAGAACTT+TGG - chr8_2:15012746-15012765 None:intergenic 25.0%
! TAGTAGACTACATTAACTAT+TGG - chr8_2:15012773-15012792 None:intergenic 25.0%
!! TATTTGAGTTCTTATCATGA+GGG - chr8_2:15011147-15011166 None:intergenic 25.0%
!! TTATTTGAGTTCTTATCATG+AGG - chr8_2:15011148-15011167 None:intergenic 25.0%
! ATGCATGTATCTATATATGT+TGG - chr8_2:15011178-15011197 None:intergenic 25.0%
! GATGTTTGAATTAGACAATA+TGG + chr8_2:15011264-15011283 Msa1236320:CDS 25.0%
! AGAAAAACAAGAATACAAAG+AGG + chr8_2:15011368-15011387 Msa1236320:CDS 25.0%
! TGATGAACAAATCAAATCAT+TGG + chr8_2:15011396-15011415 Msa1236320:CDS 25.0%
! AAAGTTTGAATCTATGAAGA+AGG + chr8_2:15011600-15011619 Msa1236320:CDS 25.0%
! AAGACAAACATTACTAATAC+AGG + chr8_2:15011624-15011643 Msa1236320:CDS 25.0%
! ATCTTGTCAACATTTCAAAA+TGG - chr8_2:15011749-15011768 None:intergenic 25.0%
!! AATCAAGTTGTGATTATCAA+TGG + chr8_2:15012306-15012325 Msa1236320:CDS 25.0%
!!! CAACATAATCATTAGTTTGT+AGG + chr8_2:15012469-15012488 Msa1236320:three_prime_UTR 25.0%
! CATTAAAAGAACTTTGGTTA+TGG - chr8_2:15012740-15012759 None:intergenic 25.0%
! TGTCAACATTAAAAGAACTT+TGG - chr8_2:15012746-15012765 None:intergenic 25.0%
! TAGTAGACTACATTAACTAT+TGG - chr8_2:15012773-15012792 None:intergenic 25.0%
! ATTTGAGTTCTTATCATGAG+GGG - chr8_2:15011146-15011165 None:intergenic 30.0%
AACATCATATGAATTAGGTG+AGG - chr8_2:15011250-15011269 None:intergenic 30.0%
!! CTTGTTTTTCTTCTTTCTCA+TGG - chr8_2:15011359-15011378 None:intergenic 30.0%
TTCAAAACAAAAGAGCTAGA+TGG + chr8_2:15011515-15011534 Msa1236320:CDS 30.0%
TGTTCTGATCTTTCTATTGA+AGG - chr8_2:15012128-15012147 None:intergenic 30.0%
AGCATAAAGGTTGAATACTT+TGG + chr8_2:15012179-15012198 Msa1236320:CDS 30.0%
! TTTGGTTTAGAAGATGAAAC+TGG + chr8_2:15012197-15012216 Msa1236320:CDS 30.0%
AAGTTGTGATTATCAATGGT+GGG + chr8_2:15012310-15012329 Msa1236320:CDS 30.0%
! TTTTGTTGAAGAAGTTGTCA+GGG + chr8_2:15012416-15012435 Msa1236320:three_prime_UTR 30.0%
! TTGTTTTCAAAGGCTATTCT+GGG + chr8_2:15012606-15012625 Msa1236320:three_prime_UTR 30.0%
TATGGCTTAAAAGGTCTATT+TGG - chr8_2:15012722-15012741 None:intergenic 30.0%
AACTTTGGTTATGGCTTAAA+AGG - chr8_2:15012731-15012750 None:intergenic 30.0%
! ATTTGAGTTCTTATCATGAG+GGG - chr8_2:15011146-15011165 None:intergenic 30.0%
AACATCATATGAATTAGGTG+AGG - chr8_2:15011250-15011269 None:intergenic 30.0%
!! CTTGTTTTTCTTCTTTCTCA+TGG - chr8_2:15011359-15011378 None:intergenic 30.0%
TTCAAAACAAAAGAGCTAGA+TGG + chr8_2:15011515-15011534 Msa1236320:CDS 30.0%
TGTTCTGATCTTTCTATTGA+AGG - chr8_2:15012128-15012147 None:intergenic 30.0%
AGCATAAAGGTTGAATACTT+TGG + chr8_2:15012179-15012198 Msa1236320:CDS 30.0%
! TTTGGTTTAGAAGATGAAAC+TGG + chr8_2:15012197-15012216 Msa1236320:CDS 30.0%
AAGTTGTGATTATCAATGGT+GGG + chr8_2:15012310-15012329 Msa1236320:CDS 30.0%
! TTTTGTTGAAGAAGTTGTCA+GGG + chr8_2:15012416-15012435 Msa1236320:three_prime_UTR 30.0%
! TTGTTTTCAAAGGCTATTCT+GGG + chr8_2:15012606-15012625 Msa1236320:three_prime_UTR 30.0%
TATGGCTTAAAAGGTCTATT+TGG - chr8_2:15012722-15012741 None:intergenic 30.0%
AACTTTGGTTATGGCTTAAA+AGG - chr8_2:15012731-15012750 None:intergenic 30.0%
GATACATGCATATCATCACA+AGG + chr8_2:15011187-15011206 Msa1236320:five_prime_UTR 35.0%
!!! AGGTGAGGAATGTTTTTTGA+GGG - chr8_2:15011235-15011254 None:intergenic 35.0%
!!! TAGGTGAGGAATGTTTTTTG+AGG - chr8_2:15011236-15011255 None:intergenic 35.0%
TATAGTTATTCAGCAGAAGC+AGG + chr8_2:15011298-15011317 Msa1236320:CDS 35.0%
GCTAATTGCAACTTCTTTCT+TGG - chr8_2:15011454-15011473 None:intergenic 35.0%
AACCATATAGCAACTTGTCT+TGG - chr8_2:15011496-15011515 None:intergenic 35.0%
! GATTGACTTTGTATCTTCAG+TGG - chr8_2:15011833-15011852 None:intergenic 35.0%
CTAATAACCATCGTGTTCAT+CGG - chr8_2:15011875-15011894 None:intergenic 35.0%
GATGAACACGATGGTTATTA+GGG + chr8_2:15011874-15011893 Msa1236320:intron 35.0%
AGTTAAAGAAGCAAACAGCA+TGG + chr8_2:15012046-15012065 Msa1236320:CDS 35.0%
TGGAGGAAAAATCAAATGTG+AGG + chr8_2:15012085-15012104 Msa1236320:CDS 35.0%
AAAAATCAAATGTGAGGCTG+AGG + chr8_2:15012091-15012110 Msa1236320:CDS 35.0%
GATCTTTCTATTGAAGGACT+TGG - chr8_2:15012122-15012141 None:intergenic 35.0%
AGATGATGACACAAGCATAA+AGG + chr8_2:15012166-15012185 Msa1236320:CDS 35.0%
! AATTTTGCTGAACATGCTGA+TGG + chr8_2:15012227-15012246 Msa1236320:CDS 35.0%
CTTTAACATCACCAGAAGAT+TGG + chr8_2:15012252-15012271 Msa1236320:CDS 35.0%
CAAGTTGTGATTATCAATGG+TGG + chr8_2:15012309-15012328 Msa1236320:CDS 35.0%
!! ATTATCAATGGTGGGACTTT+TGG + chr8_2:15012318-15012337 Msa1236320:CDS 35.0%
!! TTTGGTCTTGAATGAAACAG+AGG + chr8_2:15012336-15012355 Msa1236320:exon 35.0%
!! GTTTTGTTGAAGAAGTTGTC+AGG + chr8_2:15012415-15012434 Msa1236320:three_prime_UTR 35.0%
TTTGTTGAAGAAGTTGTCAG+GGG + chr8_2:15012417-15012436 Msa1236320:three_prime_UTR 35.0%
! GGAAATGATTTTCATCTCTG+CGG + chr8_2:15012490-15012509 Msa1236320:three_prime_UTR 35.0%
!! GTTGTTTTCAAAGGCTATTC+TGG + chr8_2:15012605-15012624 Msa1236320:three_prime_UTR 35.0%
! TGTTTTCAAAGGCTATTCTG+GGG + chr8_2:15012607-15012626 Msa1236320:three_prime_UTR 35.0%
AGTTTATGGAAGTAAGCTGA+TGG + chr8_2:15012630-15012649 Msa1236320:three_prime_UTR 35.0%
GTTTATGGAAGTAAGCTGAT+GGG + chr8_2:15012631-15012650 Msa1236320:three_prime_UTR 35.0%
GATACATGCATATCATCACA+AGG + chr8_2:15011187-15011206 Msa1236320:five_prime_UTR 35.0%
!!! AGGTGAGGAATGTTTTTTGA+GGG - chr8_2:15011235-15011254 None:intergenic 35.0%
!!! TAGGTGAGGAATGTTTTTTG+AGG - chr8_2:15011236-15011255 None:intergenic 35.0%
TATAGTTATTCAGCAGAAGC+AGG + chr8_2:15011298-15011317 Msa1236320:CDS 35.0%
GCTAATTGCAACTTCTTTCT+TGG - chr8_2:15011454-15011473 None:intergenic 35.0%
AACCATATAGCAACTTGTCT+TGG - chr8_2:15011496-15011515 None:intergenic 35.0%
! GATTGACTTTGTATCTTCAG+TGG - chr8_2:15011833-15011852 None:intergenic 35.0%
CTAATAACCATCGTGTTCAT+CGG - chr8_2:15011875-15011894 None:intergenic 35.0%
GATGAACACGATGGTTATTA+GGG + chr8_2:15011874-15011893 Msa1236320:intron 35.0%
AGTTAAAGAAGCAAACAGCA+TGG + chr8_2:15012046-15012065 Msa1236320:CDS 35.0%
TGGAGGAAAAATCAAATGTG+AGG + chr8_2:15012085-15012104 Msa1236320:CDS 35.0%
AAAAATCAAATGTGAGGCTG+AGG + chr8_2:15012091-15012110 Msa1236320:CDS 35.0%
GATCTTTCTATTGAAGGACT+TGG - chr8_2:15012122-15012141 None:intergenic 35.0%
AGATGATGACACAAGCATAA+AGG + chr8_2:15012166-15012185 Msa1236320:CDS 35.0%
! AATTTTGCTGAACATGCTGA+TGG + chr8_2:15012227-15012246 Msa1236320:CDS 35.0%
CTTTAACATCACCAGAAGAT+TGG + chr8_2:15012252-15012271 Msa1236320:CDS 35.0%
CAAGTTGTGATTATCAATGG+TGG + chr8_2:15012309-15012328 Msa1236320:CDS 35.0%
!! ATTATCAATGGTGGGACTTT+TGG + chr8_2:15012318-15012337 Msa1236320:CDS 35.0%
!! TTTGGTCTTGAATGAAACAG+AGG + chr8_2:15012336-15012355 Msa1236320:exon 35.0%
!! GTTTTGTTGAAGAAGTTGTC+AGG + chr8_2:15012415-15012434 Msa1236320:three_prime_UTR 35.0%
TTTGTTGAAGAAGTTGTCAG+GGG + chr8_2:15012417-15012436 Msa1236320:three_prime_UTR 35.0%
! GGAAATGATTTTCATCTCTG+CGG + chr8_2:15012490-15012509 Msa1236320:three_prime_UTR 35.0%
!! GTTGTTTTCAAAGGCTATTC+TGG + chr8_2:15012605-15012624 Msa1236320:three_prime_UTR 35.0%
! TGTTTTCAAAGGCTATTCTG+GGG + chr8_2:15012607-15012626 Msa1236320:three_prime_UTR 35.0%
AGTTTATGGAAGTAAGCTGA+TGG + chr8_2:15012630-15012649 Msa1236320:three_prime_UTR 35.0%
GTTTATGGAAGTAAGCTGAT+GGG + chr8_2:15012631-15012650 Msa1236320:three_prime_UTR 35.0%
TTGCAATTAGCTAGAGAGCT+TGG + chr8_2:15011463-15011482 Msa1236320:CDS 40.0%
AGCCAAGACAAGTTGCTATA+TGG + chr8_2:15011491-15011510 Msa1236320:CDS 40.0%
CGATGAACACGATGGTTATT+AGG + chr8_2:15011873-15011892 Msa1236320:intron 40.0%
CTACTCTGACTTTGCTCTAT+TGG - chr8_2:15012020-15012039 None:intergenic 40.0%
! ATGGAAAGTGCAGCAGAAAA+TGG + chr8_2:15012065-15012084 Msa1236320:CDS 40.0%
!! TCAAAAGCACTCCAATCTTC+TGG - chr8_2:15012266-15012285 None:intergenic 40.0%
ACAGAGGAGGCAAAATTATG+TGG + chr8_2:15012352-15012371 Msa1236320:three_prime_UTR 40.0%
AAGTTGTCAGGGGAAAATCA+AGG + chr8_2:15012427-15012446 Msa1236320:three_prime_UTR 40.0%
AGTTGTCAGGGGAAAATCAA+GGG + chr8_2:15012428-15012447 Msa1236320:three_prime_UTR 40.0%
! TGGCTACTTGTTACACATTC+AGG - chr8_2:15012849-15012868 None:intergenic 40.0%
TTGCAATTAGCTAGAGAGCT+TGG + chr8_2:15011463-15011482 Msa1236320:CDS 40.0%
AGCCAAGACAAGTTGCTATA+TGG + chr8_2:15011491-15011510 Msa1236320:CDS 40.0%
CGATGAACACGATGGTTATT+AGG + chr8_2:15011873-15011892 Msa1236320:intron 40.0%
CTACTCTGACTTTGCTCTAT+TGG - chr8_2:15012020-15012039 None:intergenic 40.0%
! ATGGAAAGTGCAGCAGAAAA+TGG + chr8_2:15012065-15012084 Msa1236320:CDS 40.0%
!! TCAAAAGCACTCCAATCTTC+TGG - chr8_2:15012266-15012285 None:intergenic 40.0%
ACAGAGGAGGCAAAATTATG+TGG + chr8_2:15012352-15012371 Msa1236320:three_prime_UTR 40.0%
AAGTTGTCAGGGGAAAATCA+AGG + chr8_2:15012427-15012446 Msa1236320:three_prime_UTR 40.0%
AGTTGTCAGGGGAAAATCAA+GGG + chr8_2:15012428-15012447 Msa1236320:three_prime_UTR 40.0%
! TGGCTACTTGTTACACATTC+AGG - chr8_2:15012849-15012868 None:intergenic 40.0%
GTTCATCGGTATCAACTCAG+TGG - chr8_2:15011861-15011880 None:intergenic 45.0%
GTTGATACCGATGAACACGA+TGG + chr8_2:15011865-15011884 Msa1236320:intron 45.0%
! GAAAGTGCAGCAGAAAATGG+AGG + chr8_2:15012068-15012087 Msa1236320:CDS 45.0%
GGTCTTGAATGAAACAGAGG+AGG + chr8_2:15012339-15012358 Msa1236320:exon 45.0%
GAGGAGGCAAAATTATGTGG+AGG + chr8_2:15012355-15012374 Msa1236320:three_prime_UTR 45.0%
CTCTGCGGTTTGATCATGTT+TGG + chr8_2:15012505-15012524 Msa1236320:three_prime_UTR 45.0%
! AGGCTATTCTGGGGAGTTTA+TGG + chr8_2:15012616-15012635 Msa1236320:three_prime_UTR 45.0%
GTTCATCGGTATCAACTCAG+TGG - chr8_2:15011861-15011880 None:intergenic 45.0%
GTTGATACCGATGAACACGA+TGG + chr8_2:15011865-15011884 Msa1236320:intron 45.0%
! GAAAGTGCAGCAGAAAATGG+AGG + chr8_2:15012068-15012087 Msa1236320:CDS 45.0%
GGTCTTGAATGAAACAGAGG+AGG + chr8_2:15012339-15012358 Msa1236320:exon 45.0%
GAGGAGGCAAAATTATGTGG+AGG + chr8_2:15012355-15012374 Msa1236320:three_prime_UTR 45.0%
CTCTGCGGTTTGATCATGTT+TGG + chr8_2:15012505-15012524 Msa1236320:three_prime_UTR 45.0%
! AGGCTATTCTGGGGAGTTTA+TGG + chr8_2:15012616-15012635 Msa1236320:three_prime_UTR 45.0%
!! ATTAAAAATTAAAATTGAAT+TGG - chr8_2:15011726-15011745 None:intergenic 5.0%
!! ATTAAAAATTAAAATTGAAT+TGG - chr8_2:15011726-15011745 None:intergenic 5.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 15011139 15012870 15011139 ID=Msa1236320;Name=Msa1236320
chr8_2 mRNA 15011139 15012870 15011139 ID=Msa1236320-mRNA-1;Parent=Msa1236320;Name=Msa1236320-mRNA-1;_AED=0.00;_eAED=0.00;_QI=123|1|1|1|1|1|2|524|247
chr8_2 exon 15011139 15011645 15011139 ID=Msa1236320-mRNA-1:exon:5754;Parent=Msa1236320-mRNA-1
chr8_2 exon 15011987 15012870 15011987 ID=Msa1236320-mRNA-1:exon:5755;Parent=Msa1236320-mRNA-1
chr8_2 five_prime_UTR 15011139 15011261 15011139 ID=Msa1236320-mRNA-1:five_prime_utr;Parent=Msa1236320-mRNA-1
chr8_2 CDS 15011262 15011645 15011262 ID=Msa1236320-mRNA-1:cds;Parent=Msa1236320-mRNA-1
chr8_2 CDS 15011987 15012346 15011987 ID=Msa1236320-mRNA-1:cds;Parent=Msa1236320-mRNA-1
chr8_2 three_prime_UTR 15012347 15012870 15012347 ID=Msa1236320-mRNA-1:three_prime_utr;Parent=Msa1236320-mRNA-1
Gene Sequence

>Msa1236320

ATGATGTTTGAATTAGACAATATGGAATATAGTCAATATAGTTATTCAGCAGAAGCAGGAGAAGAAGAAACATACACAACTAGTAGCACATCTTCCATGAGAAAGAAGAAAAACAAGAATACAAAGAGGTTTAGTGATGAACAAATCAAATCATTGGAAACTATGTTTGAGACTGAGACAAGACTTGAACCAAGAAAGAAGTTGCAATTAGCTAGAGAGCTTGGATTGCAGCCAAGACAAGTTGCTATATGGTTTCAAAACAAAAGAGCTAGATGGAAATCAAAGCAACTTGAAAGAGAATACAACAAACTTCAAAATAGTTACAATAATTTGGCTTCAAAGTTTGAATCTATGAAGAAGGAAAGACAAACATTACTAATACAGTTGCAGAAGCTGAATGATCTAATACAAAAGCCAATAGAGCAAAGTCAGAGTAGTTCACAAGTTAAAGAAGCAAACAGCATGGAAAGTGCAGCAGAAAATGGAGGAAAAATCAAATGTGAGGCTGAGGTGAAACCAAGTCCTTCAATAGAAAGATCAGAACATGTACTTGATGTTCTATCAGATGATGACACAAGCATAAAGGTTGAATACTTTGGTTTAGAAGATGAAACTGGTCTTATGAATTTTGCTGAACATGCTGATGGTTCTTTAACATCACCAGAAGATTGGAGTGCTTTTGAATCAAATGATTTGTTAGGTCAATCAAGTTGTGATTATCAATGGTGGGACTTTTGGTCTTGA
Protein sequence

>Msa1236320

MMFELDNMEYSQYSYSAEAGEEETYTTSSTSSMRKKKNKNTKRFSDEQIKSLETMFETETRLEPRKKLQLARELGLQPRQVAIWFQNKRARWKSKQLEREYNKLQNSYNNLASKFESMKKERQTLLIQLQKLNDLIQKPIEQSQSSSQVKEANSMESAAENGGKIKCEAEVKPSPSIERSEHVLDVLSDDDTSIKVEYFGLEDETGLMNFAEHADGSLTSPEDWSAFESNDLLGQSSCDYQWWDFWS*