AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1316250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1316250 MtrunA17_Chr8g0346251 96.371 248 8 1 1 247 1 248 8.48e-178 488
Msa1316250 MtrunA17_Chr3g0127511 43.062 209 113 3 42 247 40 245 6.22e-48 158
Msa1316250 MtrunA17_Chr6g0450991 53.125 96 45 0 42 137 69 164 1.07e-29 112
Msa1316250 MtrunA17_Chr8g0381741 48.148 108 56 0 42 149 56 163 3.45e-29 111
Msa1316250 MtrunA17_Chr6g0453021 50.000 112 47 2 21 132 86 188 1.37e-26 103
Msa1316250 MtrunA17_Chr3g0123691 38.547 179 97 6 20 194 44 213 3.23e-26 103
Msa1316250 MtrunA17_Chr8g0362151 40.945 127 70 1 8 134 50 171 5.23e-26 102
Msa1316250 MtrunA17_Chr5g0415941 48.571 105 53 1 42 145 89 193 7.15e-26 103
Msa1316250 MtrunA17_Chr8g0362751 37.222 180 90 6 42 204 91 264 2.84e-25 100
Msa1316250 MtrunA17_Chr4g0060031 42.478 113 62 1 42 154 97 206 6.54e-25 100
Msa1316250 MtrunA17_Chr5g0416391 40.984 122 72 0 13 134 50 171 7.60e-25 99.8
Msa1316250 MtrunA17_Chr1g0179581 52.381 84 33 1 42 125 30 106 3.17e-24 93.6
Msa1316250 MtrunA17_Chr3g0119291 47.253 91 48 0 42 132 61 151 1.22e-23 95.9
Msa1316250 MtrunA17_Chr7g0265521 48.352 91 40 1 42 132 65 148 6.49e-22 90.9
Msa1316250 MtrunA17_Chr7g0217111 50.000 90 38 1 43 132 83 165 3.14e-21 89.7
Msa1316250 MtrunA17_Chr5g0403701 51.852 81 34 1 28 108 46 121 3.18e-18 80.1
Msa1316250 MtrunA17_Chr5g0403691 51.852 81 34 1 28 108 46 121 1.07e-17 79.0
Msa1316250 MtrunA17_Chr4g0055851 44.578 83 46 0 43 125 123 205 2.64e-14 70.9
Msa1316250 MtrunA17_Chr7g0258831 40.741 108 59 1 18 125 143 245 4.63e-14 70.1
Msa1316250 MtrunA17_Chr4g0071411 36.975 119 67 2 43 160 91 202 6.14e-14 68.9
Msa1316250 MtrunA17_Chr1g0175651 36.667 120 72 1 6 125 124 239 9.88e-13 66.6
Msa1316250 MtrunA17_Chr5g0400781 35.200 125 70 3 43 163 126 243 1.68e-12 65.5
Msa1316250 MtrunA17_Chr5g0399491 42.169 83 48 0 43 125 139 221 7.33e-12 63.9
Msa1316250 MtrunA17_Chr2g0308611 41.111 90 50 1 39 125 196 285 1.04e-11 63.9
Msa1316250 MtrunA17_Chr4g0054421 42.169 83 48 0 43 125 130 212 1.77e-11 62.8
Msa1316250 MtrunA17_Chr3g0135331 31.707 123 80 2 43 162 148 269 2.12e-11 62.8
Msa1316250 MtrunA17_Chr8g0335131 42.169 83 48 0 43 125 149 231 3.54e-11 62.0
Msa1316250 MtrunA17_Chr1g0151971 40.964 83 49 0 43 125 133 215 5.72e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1316250 AT2G46680.1 49.012 253 108 6 15 247 7 258 1.51e-64 202
Msa1316250 AT3G61890.1 55.238 210 82 5 43 247 33 235 3.89e-63 197
Msa1316250 AT2G46680.2 51.754 228 87 7 40 247 32 256 3.80e-61 193
Msa1316250 AT3G01470.1 44.531 128 70 1 42 168 70 197 5.78e-31 115
Msa1316250 AT2G22430.1 52.747 91 43 0 42 132 64 154 1.71e-28 110
Msa1316250 AT4G40060.1 50.549 91 45 0 42 132 61 151 3.81e-28 108
Msa1316250 AT5G65310.2 48.113 106 49 1 27 132 47 146 1.75e-27 107
Msa1316250 AT5G65310.1 48.113 106 49 1 27 132 65 164 2.42e-27 107
Msa1316250 AT5G15150.1 39.041 146 78 3 18 162 96 231 5.03e-26 103
Msa1316250 AT1G69780.1 45.946 111 55 2 22 132 72 177 6.76e-25 100
Msa1316250 AT3G01220.1 45.536 112 51 2 21 132 71 172 1.62e-24 99.4
Msa1316250 AT4G36740.2 57.746 71 30 0 42 112 58 128 2.35e-22 92.0
Msa1316250 AT4G36740.1 57.746 71 30 0 42 112 57 127 2.45e-22 91.7
Msa1316250 AT1G26960.1 47.788 113 46 3 20 132 57 156 1.53e-21 90.5
Msa1316250 AT2G18550.1 42.017 119 66 1 42 157 63 181 2.66e-21 89.4
Msa1316250 AT5G03790.1 44.792 96 46 1 42 137 79 167 3.85e-21 89.0
Msa1316250 AT1G27045.4 45.098 102 56 0 42 143 70 171 1.58e-20 87.8
Msa1316250 AT5G03790.2 45.263 95 52 0 42 136 79 173 1.93e-20 86.3
Msa1316250 AT5G66700.1 53.521 71 33 0 42 112 73 143 3.45e-20 86.3
Msa1316250 AT1G27045.5 48.810 84 43 0 42 125 27 110 3.17e-19 82.8
Msa1316250 AT1G27045.3 48.810 84 43 0 42 125 27 110 3.17e-19 82.8
Msa1316250 AT1G27045.2 48.810 84 43 0 42 125 19 102 3.24e-19 82.4
Msa1316250 AT1G27045.1 48.810 84 43 0 42 125 70 153 3.94e-19 83.6
Msa1316250 AT2G36610.1 41.176 102 48 2 39 132 70 167 5.34e-18 79.3
Msa1316250 AT5G53980.1 41.071 112 58 2 31 142 3 106 1.10e-16 75.1
Msa1316250 AT4G37790.1 40.964 83 49 0 43 125 129 211 1.49e-11 63.2
Msa1316250 AT2G22800.1 42.169 83 48 0 43 125 116 198 1.71e-11 63.2
Msa1316250 AT2G44910.1 41.772 79 46 0 43 121 166 244 1.98e-11 63.2
Msa1316250 AT3G60390.1 41.772 79 46 0 43 121 165 243 2.01e-11 63.2
Msa1316250 AT5G06710.1 39.759 83 50 0 43 125 193 275 2.94e-11 62.8
Msa1316250 AT2G01430.1 35.455 110 67 1 16 125 119 224 3.94e-11 62.0
Msa1316250 AT4G17460.1 41.772 79 46 0 43 121 138 216 5.12e-11 61.6
Msa1316250 AT4G16780.1 41.772 79 46 0 43 121 132 210 6.42e-11 61.6

Find 53 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAAATAGTTACAATAATT+TGG 0.124744 8_4:-16381536 Msa1316250:CDS
AGGCTATTCTGGGGAGTTTA+TGG 0.159349 8_4:-16380493 Msa1316250:three_prime_UTR
AAATGTGATAATAGTCATTT+TGG 0.188003 8_4:-16380288 Msa1316250:three_prime_UTR
TATGGCTTAAAAGGTCTATT+TGG 0.197309 8_4:+16380390 None:intergenic
CTACTCTGACTTTGCTCTAT+TGG 0.221851 8_4:+16381092 None:intergenic
GCTAATTGCAACTTCTTTCT+TGG 0.222692 8_4:+16381658 None:intergenic
AACTTTGGTTATGGCTTAAA+AGG 0.223392 8_4:+16380381 None:intergenic
TGGCTACTTGTTACACATTC+AGG 0.269451 8_4:+16380263 None:intergenic
ATTCAAACATCATATGAATT+AGG 0.288055 8_4:+16381857 None:intergenic
ATTATCAATGGTGGGACTTT+TGG 0.297638 8_4:-16380791 Msa1316250:CDS
CTCTGCGGTTTGATCATGTT+TGG 0.309960 8_4:-16380604 Msa1316250:three_prime_UTR
TTTGAATCAAATGATTTGTT+AGG 0.319546 8_4:-16380828 Msa1316250:CDS
AGCCAAGACAAGTTGCTATA+TGG 0.331941 8_4:-16381618 Msa1316250:CDS
CATTAAAAGAACTTTGGTTA+TGG 0.336793 8_4:+16380372 None:intergenic
GATGTTTGAATTAGACAATA+TGG 0.344720 8_4:-16381845 Msa1316250:CDS
CAACATAATCATTAGTTTGT+AGG 0.346723 8_4:-16380640 Msa1316250:three_prime_UTR
TCAAAAGCACTCCAATCTTC+TGG 0.368233 8_4:+16380846 None:intergenic
TGTTCTGATCTTTCTATTGA+AGG 0.372040 8_4:+16380984 None:intergenic
ATGGAAAGTGCAGCAGAAAA+TGG 0.377944 8_4:-16381044 Msa1316250:CDS
CTTTAACATCACCAGAAGAT+TGG 0.390865 8_4:-16380857 Msa1316250:CDS
TGATGAACAAATCAAATCAT+TGG 0.409448 8_4:-16381713 Msa1316250:CDS
TTTGGTTTAGAAGATGAAAC+TGG 0.409611 8_4:-16380912 Msa1316250:CDS
AGCATAAAGGTTGAATACTT+TGG 0.411821 8_4:-16380930 Msa1316250:CDS
TGTCAACATTAAAAGAACTT+TGG 0.430634 8_4:+16380366 None:intergenic
TTCAAAACAAAAGAGCTAGA+TGG 0.440130 8_4:-16381594 Msa1316250:CDS
TTGCAATTAGCTAGAGAGCT+TGG 0.453561 8_4:-16381646 Msa1316250:CDS
AAAGTTTGAATCTATGAAGA+AGG 0.456244 8_4:-16381509 Msa1316250:CDS
AGATGATGACACAAGCATAA+AGG 0.461800 8_4:-16380943 Msa1316250:CDS
AAGACAAACATTACTAATAC+AGG 0.463639 8_4:-16381485 Msa1316250:intron
AACCATATAGCAACTTGTCT+TGG 0.480304 8_4:+16381616 None:intergenic
GATCTTTCTATTGAAGGACT+TGG 0.483251 8_4:+16380990 None:intergenic
TATAGTTATTCAGCAGAAGC+AGG 0.509566 8_4:-16381811 Msa1316250:CDS
AATCAAGTTGTGATTATCAA+TGG 0.520398 8_4:-16380803 Msa1316250:CDS
TAGTAGACTACATTAACTAT+TGG 0.524007 8_4:+16380339 None:intergenic
TTATTTGAGTTCTTATCATG+AGG 0.553646 8_4:+16381964 None:intergenic
CAAGTTGTGATTATCAATGG+TGG 0.557189 8_4:-16380800 Msa1316250:CDS
TATTTGAGTTCTTATCATGA+GGG 0.572779 8_4:+16381965 None:intergenic
AGTTTATGGAAGTAAGCTGA+TGG 0.580260 8_4:-16380479 Msa1316250:three_prime_UTR
ACAGAGGAGGCAAAATTATG+TGG 0.582377 8_4:-16380757 Msa1316250:three_prime_UTR
AAGTTGTCAGGGGAAAATCA+AGG 0.603486 8_4:-16380682 Msa1316250:three_prime_UTR
AAGTTGTGATTATCAATGGT+GGG 0.604345 8_4:-16380799 Msa1316250:CDS
AGTTGTCAGGGGAAAATCAA+GGG 0.618503 8_4:-16380681 Msa1316250:three_prime_UTR
GGTCTTGAATGAAACAGAGG+AGG 0.619886 8_4:-16380770 Msa1316250:exon
TTTGGTCTTGAATGAAACAG+AGG 0.622140 8_4:-16380773 Msa1316250:exon
ATGCATGTATCTATATATGT+TGG 0.622370 8_4:+16381934 None:intergenic
ATTTGAGTTCTTATCATGAG+GGG 0.634012 8_4:+16381966 None:intergenic
AGTTAAAGAAGCAAACAGCA+TGG 0.635070 8_4:-16381063 Msa1316250:CDS
GTTTATGGAAGTAAGCTGAT+GGG 0.635413 8_4:-16380478 Msa1316250:three_prime_UTR
TTTGTTGAAGAAGTTGTCAG+GGG 0.642443 8_4:-16380692 Msa1316250:three_prime_UTR
AACATCATATGAATTAGGTG+AGG 0.651625 8_4:+16381862 None:intergenic
GAGGAGGCAAAATTATGTGG+AGG 0.659550 8_4:-16380754 Msa1316250:three_prime_UTR
GAAAGTGCAGCAGAAAATGG+AGG 0.672573 8_4:-16381041 Msa1316250:CDS
GATACATGCATATCATCACA+AGG 0.717524 8_4:-16381922 Msa1316250:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAAATAGTTACAATAATT+TGG - chr8_4:16380695-16380714 Msa1316250:three_prime_UTR 15.0%
!! TGCGAAATTATTATTAATAA+CGG - chr8_4:16380895-16380914 Msa1316250:CDS 15.0%
!! TCAAAATAGTTACAATAATT+TGG - chr8_4:16380695-16380714 Msa1316250:three_prime_UTR 15.0%
!! TGCGAAATTATTATTAATAA+CGG - chr8_4:16380895-16380914 Msa1316250:CDS 15.0%
!! ATTCAAACATCATATGAATT+AGG + chr8_4:16380377-16380396 None:intergenic 20.0%
!!! ATAAATTTGTTTGCTTAGAA+TGG - chr8_4:16380803-16380822 Msa1316250:CDS 20.0%
!! GAAATTATTATTAATAACGG+TGG - chr8_4:16380898-16380917 Msa1316250:CDS 20.0%
!! TAATCATATAGTAATTTGAC+TGG - chr8_4:16381070-16381089 Msa1316250:CDS 20.0%
!!! TTTGAATCAAATGATTTGTT+AGG - chr8_4:16381403-16381422 Msa1316250:intron 20.0%
!!! AAGTTATAAGTTGTTTTCAA+AGG - chr8_4:16381718-16381737 Msa1316250:CDS 20.0%
!!! AAATGTGATAATAGTCATTT+TGG - chr8_4:16381943-16381962 Msa1316250:five_prime_UTR 20.0%
!! ATTCAAACATCATATGAATT+AGG + chr8_4:16380377-16380396 None:intergenic 20.0%
!!! ATAAATTTGTTTGCTTAGAA+TGG - chr8_4:16380803-16380822 Msa1316250:CDS 20.0%
!! GAAATTATTATTAATAACGG+TGG - chr8_4:16380898-16380917 Msa1316250:CDS 20.0%
!! TAATCATATAGTAATTTGAC+TGG - chr8_4:16381070-16381089 Msa1316250:CDS 20.0%
!!! TTTGAATCAAATGATTTGTT+AGG - chr8_4:16381403-16381422 Msa1316250:intron 20.0%
!!! AAGTTATAAGTTGTTTTCAA+AGG - chr8_4:16381718-16381737 Msa1316250:CDS 20.0%
!!! AAATGTGATAATAGTCATTT+TGG - chr8_4:16381943-16381962 Msa1316250:five_prime_UTR 20.0%
!! TATTTGAGTTCTTATCATGA+GGG + chr8_4:16380269-16380288 None:intergenic 25.0%
!! TTATTTGAGTTCTTATCATG+AGG + chr8_4:16380270-16380289 None:intergenic 25.0%
! ATGCATGTATCTATATATGT+TGG + chr8_4:16380300-16380319 None:intergenic 25.0%
! GATGTTTGAATTAGACAATA+TGG - chr8_4:16380386-16380405 Msa1316250:three_prime_UTR 25.0%
! AGAAAAACAAGAATACAAAG+AGG - chr8_4:16380490-16380509 Msa1316250:three_prime_UTR 25.0%
! TGATGAACAAATCAAATCAT+TGG - chr8_4:16380518-16380537 Msa1316250:three_prime_UTR 25.0%
! AAAGTTTGAATCTATGAAGA+AGG - chr8_4:16380722-16380741 Msa1316250:three_prime_UTR 25.0%
! AAGACAAACATTACTAATAC+AGG - chr8_4:16380746-16380765 Msa1316250:three_prime_UTR 25.0%
! ATCTTGTCAACATTTCAAAA+TGG + chr8_4:16380871-16380890 None:intergenic 25.0%
!! AATCAAGTTGTGATTATCAA+TGG - chr8_4:16381428-16381447 Msa1316250:intron 25.0%
!!! CAACATAATCATTAGTTTGT+AGG - chr8_4:16381591-16381610 Msa1316250:CDS 25.0%
! CATTAAAAGAACTTTGGTTA+TGG + chr8_4:16381862-16381881 None:intergenic 25.0%
! TGTCAACATTAAAAGAACTT+TGG + chr8_4:16381868-16381887 None:intergenic 25.0%
! TAGTAGACTACATTAACTAT+TGG + chr8_4:16381895-16381914 None:intergenic 25.0%
!! TATTTGAGTTCTTATCATGA+GGG + chr8_4:16380269-16380288 None:intergenic 25.0%
!! TTATTTGAGTTCTTATCATG+AGG + chr8_4:16380270-16380289 None:intergenic 25.0%
! ATGCATGTATCTATATATGT+TGG + chr8_4:16380300-16380319 None:intergenic 25.0%
! GATGTTTGAATTAGACAATA+TGG - chr8_4:16380386-16380405 Msa1316250:three_prime_UTR 25.0%
! AGAAAAACAAGAATACAAAG+AGG - chr8_4:16380490-16380509 Msa1316250:three_prime_UTR 25.0%
! TGATGAACAAATCAAATCAT+TGG - chr8_4:16380518-16380537 Msa1316250:three_prime_UTR 25.0%
! AAAGTTTGAATCTATGAAGA+AGG - chr8_4:16380722-16380741 Msa1316250:three_prime_UTR 25.0%
! AAGACAAACATTACTAATAC+AGG - chr8_4:16380746-16380765 Msa1316250:three_prime_UTR 25.0%
! ATCTTGTCAACATTTCAAAA+TGG + chr8_4:16380871-16380890 None:intergenic 25.0%
!! AATCAAGTTGTGATTATCAA+TGG - chr8_4:16381428-16381447 Msa1316250:intron 25.0%
!!! CAACATAATCATTAGTTTGT+AGG - chr8_4:16381591-16381610 Msa1316250:CDS 25.0%
! CATTAAAAGAACTTTGGTTA+TGG + chr8_4:16381862-16381881 None:intergenic 25.0%
! TGTCAACATTAAAAGAACTT+TGG + chr8_4:16381868-16381887 None:intergenic 25.0%
! TAGTAGACTACATTAACTAT+TGG + chr8_4:16381895-16381914 None:intergenic 25.0%
! ATTTGAGTTCTTATCATGAG+GGG + chr8_4:16380268-16380287 None:intergenic 30.0%
AACATCATATGAATTAGGTG+AGG + chr8_4:16380372-16380391 None:intergenic 30.0%
!! CTTGTTTTTCTTCTTTCTCA+TGG + chr8_4:16380481-16380500 None:intergenic 30.0%
TTCAAAACAAAAGAGCTAGA+TGG - chr8_4:16380637-16380656 Msa1316250:three_prime_UTR 30.0%
TGTTCTGATCTTTCTATTGA+AGG + chr8_4:16381250-16381269 None:intergenic 30.0%
AGCATAAAGGTTGAATACTT+TGG - chr8_4:16381301-16381320 Msa1316250:intron 30.0%
! TTTGGTTTAGAAGATGAAAC+TGG - chr8_4:16381319-16381338 Msa1316250:intron 30.0%
AAGTTGTGATTATCAATGGT+GGG - chr8_4:16381432-16381451 Msa1316250:intron 30.0%
! TTTTGTTGAAGAAGTTGTCA+GGG - chr8_4:16381538-16381557 Msa1316250:CDS 30.0%
! TTGTTTTCAAAGGCTATTCT+GGG - chr8_4:16381728-16381747 Msa1316250:CDS 30.0%
TATGGCTTAAAAGGTCTATT+TGG + chr8_4:16381844-16381863 None:intergenic 30.0%
AACTTTGGTTATGGCTTAAA+AGG + chr8_4:16381853-16381872 None:intergenic 30.0%
! ATTTGAGTTCTTATCATGAG+GGG + chr8_4:16380268-16380287 None:intergenic 30.0%
AACATCATATGAATTAGGTG+AGG + chr8_4:16380372-16380391 None:intergenic 30.0%
!! CTTGTTTTTCTTCTTTCTCA+TGG + chr8_4:16380481-16380500 None:intergenic 30.0%
TTCAAAACAAAAGAGCTAGA+TGG - chr8_4:16380637-16380656 Msa1316250:three_prime_UTR 30.0%
TGTTCTGATCTTTCTATTGA+AGG + chr8_4:16381250-16381269 None:intergenic 30.0%
AGCATAAAGGTTGAATACTT+TGG - chr8_4:16381301-16381320 Msa1316250:intron 30.0%
! TTTGGTTTAGAAGATGAAAC+TGG - chr8_4:16381319-16381338 Msa1316250:intron 30.0%
AAGTTGTGATTATCAATGGT+GGG - chr8_4:16381432-16381451 Msa1316250:intron 30.0%
! TTTTGTTGAAGAAGTTGTCA+GGG - chr8_4:16381538-16381557 Msa1316250:CDS 30.0%
! TTGTTTTCAAAGGCTATTCT+GGG - chr8_4:16381728-16381747 Msa1316250:CDS 30.0%
TATGGCTTAAAAGGTCTATT+TGG + chr8_4:16381844-16381863 None:intergenic 30.0%
AACTTTGGTTATGGCTTAAA+AGG + chr8_4:16381853-16381872 None:intergenic 30.0%
GATACATGCATATCATCACA+AGG - chr8_4:16380309-16380328 Msa1316250:three_prime_UTR 35.0%
!!! AGGTGAGGAATGTTTTTTGA+GGG + chr8_4:16380357-16380376 None:intergenic 35.0%
!!! TAGGTGAGGAATGTTTTTTG+AGG + chr8_4:16380358-16380377 None:intergenic 35.0%
TATAGTTATTCAGCAGAAGC+AGG - chr8_4:16380420-16380439 Msa1316250:three_prime_UTR 35.0%
GCTAATTGCAACTTCTTTCT+TGG + chr8_4:16380576-16380595 None:intergenic 35.0%
AACCATATAGCAACTTGTCT+TGG + chr8_4:16380618-16380637 None:intergenic 35.0%
! GATTGACTTTGTATCTTCAG+TGG + chr8_4:16380955-16380974 None:intergenic 35.0%
CTAATAACCATCGTGTTCAT+CGG + chr8_4:16380997-16381016 None:intergenic 35.0%
GATGAACACGATGGTTATTA+GGG - chr8_4:16380996-16381015 Msa1316250:CDS 35.0%
AGTTAAAGAAGCAAACAGCA+TGG - chr8_4:16381168-16381187 Msa1316250:intron 35.0%
TGGAGGAAAAATCAAATGTG+AGG - chr8_4:16381207-16381226 Msa1316250:intron 35.0%
AAAAATCAAATGTGAGGCTG+AGG - chr8_4:16381213-16381232 Msa1316250:intron 35.0%
GATCTTTCTATTGAAGGACT+TGG + chr8_4:16381244-16381263 None:intergenic 35.0%
AGATGATGACACAAGCATAA+AGG - chr8_4:16381288-16381307 Msa1316250:intron 35.0%
! AATTTTGCTGAACATGCTGA+TGG - chr8_4:16381349-16381368 Msa1316250:intron 35.0%
CTTTAACATCACCAGAAGAT+TGG - chr8_4:16381374-16381393 Msa1316250:intron 35.0%
CAAGTTGTGATTATCAATGG+TGG - chr8_4:16381431-16381450 Msa1316250:intron 35.0%
!! ATTATCAATGGTGGGACTTT+TGG - chr8_4:16381440-16381459 Msa1316250:intron 35.0%
!! TTTGGTCTTGAATGAAACAG+AGG - chr8_4:16381458-16381477 Msa1316250:intron 35.0%
!! GTTTTGTTGAAGAAGTTGTC+AGG - chr8_4:16381537-16381556 Msa1316250:CDS 35.0%
TTTGTTGAAGAAGTTGTCAG+GGG - chr8_4:16381539-16381558 Msa1316250:CDS 35.0%
! GGAAATGATTTTCATCTCTG+CGG - chr8_4:16381612-16381631 Msa1316250:CDS 35.0%
!! GTTGTTTTCAAAGGCTATTC+TGG - chr8_4:16381727-16381746 Msa1316250:CDS 35.0%
! TGTTTTCAAAGGCTATTCTG+GGG - chr8_4:16381729-16381748 Msa1316250:CDS 35.0%
AGTTTATGGAAGTAAGCTGA+TGG - chr8_4:16381752-16381771 Msa1316250:CDS 35.0%
GTTTATGGAAGTAAGCTGAT+GGG - chr8_4:16381753-16381772 Msa1316250:CDS 35.0%
GATACATGCATATCATCACA+AGG - chr8_4:16380309-16380328 Msa1316250:three_prime_UTR 35.0%
!!! AGGTGAGGAATGTTTTTTGA+GGG + chr8_4:16380357-16380376 None:intergenic 35.0%
!!! TAGGTGAGGAATGTTTTTTG+AGG + chr8_4:16380358-16380377 None:intergenic 35.0%
TATAGTTATTCAGCAGAAGC+AGG - chr8_4:16380420-16380439 Msa1316250:three_prime_UTR 35.0%
GCTAATTGCAACTTCTTTCT+TGG + chr8_4:16380576-16380595 None:intergenic 35.0%
AACCATATAGCAACTTGTCT+TGG + chr8_4:16380618-16380637 None:intergenic 35.0%
! GATTGACTTTGTATCTTCAG+TGG + chr8_4:16380955-16380974 None:intergenic 35.0%
CTAATAACCATCGTGTTCAT+CGG + chr8_4:16380997-16381016 None:intergenic 35.0%
GATGAACACGATGGTTATTA+GGG - chr8_4:16380996-16381015 Msa1316250:CDS 35.0%
AGTTAAAGAAGCAAACAGCA+TGG - chr8_4:16381168-16381187 Msa1316250:intron 35.0%
TGGAGGAAAAATCAAATGTG+AGG - chr8_4:16381207-16381226 Msa1316250:intron 35.0%
AAAAATCAAATGTGAGGCTG+AGG - chr8_4:16381213-16381232 Msa1316250:intron 35.0%
GATCTTTCTATTGAAGGACT+TGG + chr8_4:16381244-16381263 None:intergenic 35.0%
AGATGATGACACAAGCATAA+AGG - chr8_4:16381288-16381307 Msa1316250:intron 35.0%
! AATTTTGCTGAACATGCTGA+TGG - chr8_4:16381349-16381368 Msa1316250:intron 35.0%
CTTTAACATCACCAGAAGAT+TGG - chr8_4:16381374-16381393 Msa1316250:intron 35.0%
CAAGTTGTGATTATCAATGG+TGG - chr8_4:16381431-16381450 Msa1316250:intron 35.0%
!! ATTATCAATGGTGGGACTTT+TGG - chr8_4:16381440-16381459 Msa1316250:intron 35.0%
!! TTTGGTCTTGAATGAAACAG+AGG - chr8_4:16381458-16381477 Msa1316250:intron 35.0%
!! GTTTTGTTGAAGAAGTTGTC+AGG - chr8_4:16381537-16381556 Msa1316250:CDS 35.0%
TTTGTTGAAGAAGTTGTCAG+GGG - chr8_4:16381539-16381558 Msa1316250:CDS 35.0%
! GGAAATGATTTTCATCTCTG+CGG - chr8_4:16381612-16381631 Msa1316250:CDS 35.0%
!! GTTGTTTTCAAAGGCTATTC+TGG - chr8_4:16381727-16381746 Msa1316250:CDS 35.0%
! TGTTTTCAAAGGCTATTCTG+GGG - chr8_4:16381729-16381748 Msa1316250:CDS 35.0%
AGTTTATGGAAGTAAGCTGA+TGG - chr8_4:16381752-16381771 Msa1316250:CDS 35.0%
GTTTATGGAAGTAAGCTGAT+GGG - chr8_4:16381753-16381772 Msa1316250:CDS 35.0%
TTGCAATTAGCTAGAGAGCT+TGG - chr8_4:16380585-16380604 Msa1316250:three_prime_UTR 40.0%
AGCCAAGACAAGTTGCTATA+TGG - chr8_4:16380613-16380632 Msa1316250:three_prime_UTR 40.0%
CGATGAACACGATGGTTATT+AGG - chr8_4:16380995-16381014 Msa1316250:CDS 40.0%
CTACTCTGACTTTGCTCTAT+TGG + chr8_4:16381142-16381161 None:intergenic 40.0%
! ATGGAAAGTGCAGCAGAAAA+TGG - chr8_4:16381187-16381206 Msa1316250:intron 40.0%
!! TCAAAAGCACTCCAATCTTC+TGG + chr8_4:16381388-16381407 None:intergenic 40.0%
ACAGAGGAGGCAAAATTATG+TGG - chr8_4:16381474-16381493 Msa1316250:intron 40.0%
AAGTTGTCAGGGGAAAATCA+AGG - chr8_4:16381549-16381568 Msa1316250:CDS 40.0%
AGTTGTCAGGGGAAAATCAA+GGG - chr8_4:16381550-16381569 Msa1316250:CDS 40.0%
! TGGCTACTTGTTACACATTC+AGG + chr8_4:16381971-16381990 None:intergenic 40.0%
TTGCAATTAGCTAGAGAGCT+TGG - chr8_4:16380585-16380604 Msa1316250:three_prime_UTR 40.0%
AGCCAAGACAAGTTGCTATA+TGG - chr8_4:16380613-16380632 Msa1316250:three_prime_UTR 40.0%
CGATGAACACGATGGTTATT+AGG - chr8_4:16380995-16381014 Msa1316250:CDS 40.0%
CTACTCTGACTTTGCTCTAT+TGG + chr8_4:16381142-16381161 None:intergenic 40.0%
! ATGGAAAGTGCAGCAGAAAA+TGG - chr8_4:16381187-16381206 Msa1316250:intron 40.0%
!! TCAAAAGCACTCCAATCTTC+TGG + chr8_4:16381388-16381407 None:intergenic 40.0%
ACAGAGGAGGCAAAATTATG+TGG - chr8_4:16381474-16381493 Msa1316250:intron 40.0%
AAGTTGTCAGGGGAAAATCA+AGG - chr8_4:16381549-16381568 Msa1316250:CDS 40.0%
AGTTGTCAGGGGAAAATCAA+GGG - chr8_4:16381550-16381569 Msa1316250:CDS 40.0%
! TGGCTACTTGTTACACATTC+AGG + chr8_4:16381971-16381990 None:intergenic 40.0%
GTTCATCGGTATCAACTCAG+TGG + chr8_4:16380983-16381002 None:intergenic 45.0%
GTTGATACCGATGAACACGA+TGG - chr8_4:16380987-16381006 Msa1316250:CDS 45.0%
! GAAAGTGCAGCAGAAAATGG+AGG - chr8_4:16381190-16381209 Msa1316250:intron 45.0%
GGTCTTGAATGAAACAGAGG+AGG - chr8_4:16381461-16381480 Msa1316250:intron 45.0%
GAGGAGGCAAAATTATGTGG+AGG - chr8_4:16381477-16381496 Msa1316250:intron 45.0%
CTCTGCGGTTTGATCATGTT+TGG - chr8_4:16381627-16381646 Msa1316250:CDS 45.0%
! AGGCTATTCTGGGGAGTTTA+TGG - chr8_4:16381738-16381757 Msa1316250:CDS 45.0%
GTTCATCGGTATCAACTCAG+TGG + chr8_4:16380983-16381002 None:intergenic 45.0%
GTTGATACCGATGAACACGA+TGG - chr8_4:16380987-16381006 Msa1316250:CDS 45.0%
! GAAAGTGCAGCAGAAAATGG+AGG - chr8_4:16381190-16381209 Msa1316250:intron 45.0%
GGTCTTGAATGAAACAGAGG+AGG - chr8_4:16381461-16381480 Msa1316250:intron 45.0%
GAGGAGGCAAAATTATGTGG+AGG - chr8_4:16381477-16381496 Msa1316250:intron 45.0%
CTCTGCGGTTTGATCATGTT+TGG - chr8_4:16381627-16381646 Msa1316250:CDS 45.0%
! AGGCTATTCTGGGGAGTTTA+TGG - chr8_4:16381738-16381757 Msa1316250:CDS 45.0%
!! ATTAAAAATTAAAATTGAAT+TGG + chr8_4:16380848-16380867 None:intergenic 5.0%
!! ATTAAAAATTAAAATTGAAT+TGG + chr8_4:16380848-16380867 None:intergenic 5.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 16380261 16381992 16380261 ID=Msa1316250;Name=Msa1316250
chr8_4 mRNA 16380261 16381992 16380261 ID=Msa1316250-mRNA-1;Parent=Msa1316250;Name=Msa1316250-mRNA-1;_AED=0.00;_eAED=0.00;_QI=123|1|1|1|1|1|2|524|247
chr8_4 exon 16380261 16381144 16380261 ID=Msa1316250-mRNA-1:exon:6177;Parent=Msa1316250-mRNA-1
chr8_4 exon 16381486 16381992 16381486 ID=Msa1316250-mRNA-1:exon:6176;Parent=Msa1316250-mRNA-1
chr8_4 five_prime_UTR 16381870 16381992 16381870 ID=Msa1316250-mRNA-1:five_prime_utr;Parent=Msa1316250-mRNA-1
chr8_4 CDS 16381486 16381869 16381486 ID=Msa1316250-mRNA-1:cds;Parent=Msa1316250-mRNA-1
chr8_4 CDS 16380785 16381144 16380785 ID=Msa1316250-mRNA-1:cds;Parent=Msa1316250-mRNA-1
chr8_4 three_prime_UTR 16380261 16380784 16380261 ID=Msa1316250-mRNA-1:three_prime_utr;Parent=Msa1316250-mRNA-1
Gene Sequence

>Msa1316250

ATGATGTTTGAATTAGACAATATGGAATATAGTCAATATAGTTATTCAGCAGAAGCAGGAGAAGAAGAAACATACACAACTAGTAGCACATCTTCCATGAGAAAGAAGAAAAACAAGAATACAAAGAGGTTTAGTGATGAACAAATCAAATCATTGGAAACTATGTTTGAGACTGAGACAAGACTTGAACCAAGAAAGAAGTTGCAATTAGCTAGAGAGCTTGGATTGCAGCCAAGACAAGTTGCTATATGGTTTCAAAACAAAAGAGCTAGATGGAAATCAAAGCAACTTGAAAGAGAATACAACAAACTTCAAAATAGTTACAATAATTTGGCTTCAAAGTTTGAATCTATGAAGAAGGAAAGACAAACATTACTAATACAGTTGCAGAAGCTGAATGATCTAATACAAAAGCCAATAGAGCAAAGTCAGAGTAGTTCACAAGTTAAAGAAGCAAACAGCATGGAAAGTGCAGCAGAAAATGGAGGAAAAATCAAATGTGAGGCTGAGGTGAAACCAAGTCCTTCAATAGAAAGATCAGAACATGTACTTGATGTTCTATCAGATGATGACACAAGCATAAAGGTTGAATACTTTGGTTTAGAAGATGAAACTGGTCTTATGAATTTTGCTGAACATGCTGATGGTTCTTTAACATCACCAGAAGATTGGAGTGCTTTTGAATCAAATGATTTGTTAGGTCAATCAAGTTGTGATTATCAATGGTGGGACTTTTGGTCTTGA
Protein sequence

>Msa1316250

MMFELDNMEYSQYSYSAEAGEEETYTTSSTSSMRKKKNKNTKRFSDEQIKSLETMFETETRLEPRKKLQLARELGLQPRQVAIWFQNKRARWKSKQLEREYNKLQNSYNNLASKFESMKKERQTLLIQLQKLNDLIQKPIEQSQSSSQVKEANSMESAAENGGKIKCEAEVKPSPSIERSEHVLDVLSDDDTSIKVEYFGLEDETGLMNFAEHADGSLTSPEDWSAFESNDLLGQSSCDYQWWDFWS*