AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1259390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 85 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATGACATAAATCAAAAT+TGG 0.154406 8_2:+61018873 None:intergenic
TTGGGATTTGCTGCTGTAAA+AGG 0.245335 8_2:-61019295 Msa1259390:CDS
CATGAACAGTGTTGCGATTT+GGG 0.277970 8_2:+61021459 None:intergenic
GAAGAAAAGACCCTAAAATT+TGG 0.284411 8_2:-61020303 Msa1259390:five_prime_UTR
ACTGCTGCTTCCTTGGGTTC+TGG 0.294667 8_2:+61020631 None:intergenic
ACTTGCTACTGCTGCTTCCT+TGG 0.295570 8_2:+61020624 None:intergenic
TGATGACATAAATCAAAATT+GGG 0.308819 8_2:+61018874 None:intergenic
TTTCAATGCATCTGAAGTTA+TGG 0.314410 8_2:-61020397 Msa1259390:five_prime_UTR
AGCGACGTCGTTTGCACTCT+TGG 0.322976 8_2:+61019180 None:intergenic
TAACAGTTATCTATATAAAA+AGG 0.323600 8_2:-61019497 Msa1259390:five_prime_UTR
AAAGCAATTGTTCTGTCATC+AGG 0.325832 8_2:+61020429 None:intergenic
GGTACGTTGGATGGCTTTAA+TGG 0.329174 8_2:+61019237 None:intergenic
GACAAATTTGAGTCTGCTTT+GGG 0.329614 8_2:-61020661 Msa1259390:five_prime_UTR
TTAAGACTTGTAAAGTAGTT+AGG 0.331314 8_2:-61018703 Msa1259390:three_prime_UTR
GCGATGTTGAATCTCTCTTT+TGG 0.347039 8_2:-61018910 Msa1259390:three_prime_UTR
CGCGCACGGAACGCTGCAAC+AGG 0.350992 8_2:-61020234 Msa1259390:intron
CCATGAACAGTGTTGCGATT+TGG 0.352695 8_2:+61021458 None:intergenic
AGACAAATTTGAGTCTGCTT+TGG 0.352947 8_2:-61020662 Msa1259390:five_prime_UTR
CCAAATCGCAACACTGTTCA+TGG 0.355738 8_2:-61021458 Msa1259390:five_prime_UTR
CTTGCTACTGCTGCTTCCTT+GGG 0.371354 8_2:+61020625 None:intergenic
CAAATTATGAGTTGGTGTAT+TGG 0.382778 8_2:+61018793 None:intergenic
GCGTTTGATTCGCTAGGCTT+TGG 0.384574 8_2:-61020281 Msa1259390:five_prime_UTR
GTTGGATGGCTTTAATGGTC+TGG 0.390968 8_2:+61019242 None:intergenic
TTGATTTATGTCATCAAAGT+TGG 0.391346 8_2:-61018867 Msa1259390:three_prime_UTR
TTCAATGCATCTGAAGTTAT+GGG 0.417554 8_2:-61020396 Msa1259390:five_prime_UTR
CACTGTTCATCAGGTTCAAA+GGG 0.430310 8_2:-61021396 Msa1259390:intron
CATAATTTGAAGATATTACA+TGG 0.430630 8_2:-61018779 Msa1259390:three_prime_UTR
AAAATATCAGGGAAACAATC+TGG 0.446965 8_2:+61019216 None:intergenic
TAAAACAGTGAATTTAAAAG+AGG 0.452932 8_2:+61018941 None:intergenic
GGATGGTGTTCCAGAGGAAA+AGG 0.461035 8_2:-61020361 Msa1259390:five_prime_UTR
AATTCTGTCAAGAGAAATAA+AGG 0.467296 8_2:+61018837 None:intergenic
GTCGGCACGACGAGGTCATA+TGG 0.480658 8_2:-61019101 Msa1259390:three_prime_UTR
AGAGGTATTCAATCTACGTA+TGG 0.495214 8_2:+61018959 None:intergenic
AATGATGTAGGTACCGGATT+GGG 0.499273 8_2:-61019313 Msa1259390:intron
TTTCTCTCTAGACAACTCTT+GGG 0.503761 8_2:+61020334 None:intergenic
TCGCTAGGCTTTGGATGGCA+TGG 0.505322 8_2:-61020272 Msa1259390:five_prime_UTR
TTGATTCGCTAGGCTTTGGA+TGG 0.506861 8_2:-61020277 Msa1259390:five_prime_UTR
TAATGATGTAGGTACCGGAT+TGG 0.513302 8_2:-61019314 Msa1259390:intron
GTTTCAATCGGTGAAGAATG+AGG 0.516228 8_2:+61021536 None:intergenic
CGGTGAAGAACAGAGGGATC+TGG 0.516648 8_2:+61021509 None:intergenic
GGATCTGGTTCAGTTTCAAT+CGG 0.517490 8_2:+61021524 None:intergenic
TGCCATGTTACCAGTGGAAG+AGG 0.519937 8_2:-61019434 Msa1259390:intron
GCACTGTTCATCAGGTTCAA+AGG 0.521311 8_2:-61021397 Msa1259390:intron
AATGGCATGAGAAGCACTCA+TGG 0.522645 8_2:-61018641 Msa1259390:three_prime_UTR
TACAGCAGCAAATCCCAATC+CGG 0.525664 8_2:+61019300 None:intergenic
CGACGACGGCGCAACACCGA+TGG 0.527731 8_2:+61019060 None:intergenic
TCTTTGGTTCACAATGGAAG+AGG 0.528030 8_2:-61019465 Msa1259390:exon
TTTCAATCGGTGAAGAATGA+GGG 0.529634 8_2:+61021537 None:intergenic
AGGGAAACAATCTGGTACGT+TGG 0.537244 8_2:+61019224 None:intergenic
TTTGGATGGCATGGAGAATG+AGG 0.537359 8_2:-61020263 Msa1259390:five_prime_UTR
GACTGGTAGAGGGACGAAGA+AGG 0.539140 8_2:+61019004 None:intergenic
AAACAATCTGGTACGTTGGA+TGG 0.543310 8_2:+61019228 None:intergenic
ACTTAACTGTGTGTAACTGC+GGG 0.549402 8_2:+61019269 None:intergenic
AATATCTTCAAATTATGAGT+TGG 0.553846 8_2:+61018785 None:intergenic
TACTTAACTGTGTGTAACTG+CGG 0.560947 8_2:+61019268 None:intergenic
ACAACTTGAGGTCACTCTAT+CGG 0.561540 8_2:-61019142 Msa1259390:three_prime_UTR
TCACATAGTACCTCTTCCAC+TGG 0.564675 8_2:+61019424 None:intergenic
AAGCAATTGTTCTGTCATCA+GGG 0.565976 8_2:+61020430 None:intergenic
TACCTCTTCCACTGGTAACA+TGG 0.566570 8_2:+61019432 None:intergenic
GATGACATAAATCAAAATTG+GGG 0.567982 8_2:+61018875 None:intergenic
GTGTTGCGCCGTCGTCGAGA+AGG 0.583330 8_2:-61019054 Msa1259390:three_prime_UTR
CGGCGCAACACCGATGGACA+AGG 0.585797 8_2:+61019066 None:intergenic
GAGGTATTCAATCTACGTAT+GGG 0.587598 8_2:+61018960 None:intergenic
ATTATTTCTTTGGTTCACAA+TGG 0.591280 8_2:-61019471 Msa1259390:exon
AGAATTCGACAGAGACAAAG+AGG 0.596231 8_2:-61018820 Msa1259390:three_prime_UTR
GCGCCGTCGTCGAGAAGGAG+AGG 0.596832 8_2:-61019049 Msa1259390:three_prime_UTR
GTTCACAATGGAAGAGGACA+CGG 0.598072 8_2:-61019459 Msa1259390:exon
TTCAATCGGTGAAGAATGAG+GGG 0.601263 8_2:+61021538 None:intergenic
AGATGAAATTTCAACATGTG+TGG 0.606032 8_2:+61020497 None:intergenic
GAGATTGAATCCTTGTCCAT+CGG 0.607274 8_2:-61019076 Msa1259390:three_prime_UTR
AACCAGATCAAAACAACTTG+AGG 0.607973 8_2:-61019154 Msa1259390:three_prime_UTR
CAAATCGCAACACTGTTCAT+GGG 0.617397 8_2:-61021457 Msa1259390:intron
GGGAACAAAACCAGAACCCA+AGG 0.621328 8_2:-61020641 Msa1259390:five_prime_UTR
AATTTGGCGTTTGATTCGCT+AGG 0.629334 8_2:-61020287 Msa1259390:five_prime_UTR
TGAGAACGGTGAAGAACAGA+GGG 0.629876 8_2:+61021503 None:intergenic
AAAGAAGGATGGTGTTCCAG+AGG 0.634111 8_2:-61020367 Msa1259390:five_prime_UTR
CTGTAGGAATGCTAAATACG+AGG 0.634576 8_2:-61020692 Msa1259390:intron
AAGAACAAGAGATTTGAGAA+CGG 0.641744 8_2:+61021489 None:intergenic
CGTCGAGAAGGAGAGGTCGT+CGG 0.647129 8_2:-61019042 Msa1259390:three_prime_UTR
TTGAGAACGGTGAAGAACAG+AGG 0.652047 8_2:+61021502 None:intergenic
GAATGAGGCAAATTCGCGCA+CGG 0.657463 8_2:-61020248 Msa1259390:five_prime_UTR
CATGAATGATGAGTTGATGT+CGG 0.665319 8_2:-61019119 Msa1259390:three_prime_UTR
ACGGCTTGCCATGTTACCAG+TGG 0.669509 8_2:-61019440 Msa1259390:CDS
CGACCTCTCCTTCTCGACGA+CGG 0.677608 8_2:+61019046 None:intergenic
GAGTTGATGTCGGCACGACG+AGG 0.703590 8_2:-61019109 Msa1259390:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGAAAATAAGTTAAAAA+CGG + chr8_2:61019216-61019235 None:intergenic 10.0%
!!! TATATTTTGAATCTTAAAAT+AGG - chr8_2:61020119-61020138 Msa1259390:intron 10.0%
!!! TGTTTTTACTATAATATGTT+AGG - chr8_2:61018951-61018970 Msa1259390:three_prime_UTR 15.0%
!!! GAAAAGTGATTTTATTTTTA+TGG - chr8_2:61019058-61019077 Msa1259390:three_prime_UTR 15.0%
!!! AAAACAATATAACTTGATTT+TGG - chr8_2:61019278-61019297 Msa1259390:CDS 15.0%
!!! TTGTAGTTTTAATATCATAA+TGG + chr8_2:61019373-61019392 None:intergenic 15.0%
!! ACTAAATCAATTATGTTATT+AGG - chr8_2:61020453-61020472 Msa1259390:five_prime_UTR 15.0%
!!! AAATTAAATTTGATTTAAGC+AGG - chr8_2:61020494-61020513 Msa1259390:five_prime_UTR 15.0%
!!! AATTAAATTTGATTTAAGCA+GGG - chr8_2:61020495-61020514 Msa1259390:five_prime_UTR 15.0%
!! TAACAGTTATCTATATAAAA+AGG - chr8_2:61020645-61020664 Msa1259390:five_prime_UTR 15.0%
!! AGTAAAAACATAAAATATCA+GGG + chr8_2:61020940-61020959 None:intergenic 15.0%
!!! CAGAAGAATAATGAAATTTT+GGG + chr8_2:61018800-61018819 None:intergenic 20.0%
!!! ACAGAAGAATAATGAAATTT+TGG + chr8_2:61018801-61018820 None:intergenic 20.0%
!! AAAATGATAACAAGTGAAAA+AGG + chr8_2:61018863-61018882 None:intergenic 20.0%
!! TTGGAATTTCAATTTATCTA+GGG - chr8_2:61018999-61019018 Msa1259390:three_prime_UTR 20.0%
!!! ATGTATTTTCTCTTATATAG+TGG - chr8_2:61019127-61019146 Msa1259390:three_prime_UTR 20.0%
!! AAATATCTTTACAATAGTGA+TGG - chr8_2:61020088-61020107 Msa1259390:intron 20.0%
!! AAAATGCTATATGTTCATAT+AGG - chr8_2:61020602-61020621 Msa1259390:five_prime_UTR 20.0%
!!! AAAAAGGTTCATTATTTCTT+TGG - chr8_2:61020661-61020680 Msa1259390:five_prime_UTR 20.0%
!! ATTCCATTAAAATCAACAAA+AGG + chr8_2:61020759-61020778 None:intergenic 20.0%
!!! ATTTATTTCTGTAATGATGT+AGG - chr8_2:61020817-61020836 Msa1259390:intron 20.0%
!! CAGTAAAAACATAAAATATC+AGG + chr8_2:61020941-61020960 None:intergenic 20.0%
!! TAAAACAGTGAATTTAAAAG+AGG + chr8_2:61021204-61021223 None:intergenic 20.0%
!! TGATGACATAAATCAAAATT+GGG + chr8_2:61021271-61021290 None:intergenic 20.0%
!!! TTGATGACATAAATCAAAAT+TGG + chr8_2:61021272-61021291 None:intergenic 20.0%
!! AATATCTTCAAATTATGAGT+TGG + chr8_2:61021360-61021379 None:intergenic 20.0%
!! CATAATTTGAAGATATTACA+TGG - chr8_2:61021363-61021382 Msa1259390:intron 20.0%
!!! TTAGTTTTGTATTACTGAAA+TGG - chr8_2:61021483-61021502 Msa1259390:five_prime_UTR 20.0%
! TTTCTCAAGTTCAATCAAAT+AGG - chr8_2:61018890-61018909 Msa1259390:three_prime_UTR 25.0%
! TTCTCAAGTTCAATCAAATA+GGG - chr8_2:61018891-61018910 Msa1259390:three_prime_UTR 25.0%
!!! GAATAAGAACTACTTTTACT+TGG - chr8_2:61018980-61018999 Msa1259390:three_prime_UTR 25.0%
! CTTGGAATTTCAATTTATCT+AGG - chr8_2:61018998-61019017 Msa1259390:three_prime_UTR 25.0%
! TTTCATAAGTCATCAAAGAT+AGG - chr8_2:61019228-61019247 Msa1259390:three_prime_UTR 25.0%
! AGTTATGGGTATAAAAAAGA+AGG - chr8_2:61019760-61019779 Msa1259390:intron 25.0%
!! ATCATTTAACCAGTGTTATT+AGG - chr8_2:61020237-61020256 Msa1259390:five_prime_UTR 25.0%
!!! TTTTTTCCACTCTTATTTGT+AGG - chr8_2:61020305-61020324 Msa1259390:five_prime_UTR 25.0%
!! ATTATTTCTTTGGTTCACAA+TGG - chr8_2:61020671-61020690 Msa1259390:five_prime_UTR 25.0%
!!! TGTCCTTTTGTTGATTTTAA+TGG - chr8_2:61020753-61020772 Msa1259390:intron 25.0%
! GATGACATAAATCAAAATTG+GGG + chr8_2:61021270-61021289 None:intergenic 25.0%
! TTGATTTATGTCATCAAAGT+TGG - chr8_2:61021275-61021294 Msa1259390:intron 25.0%
! AATTCTGTCAAGAGAAATAA+AGG + chr8_2:61021308-61021327 None:intergenic 25.0%
! CTTAAAAAAATTCTGCACAA+TGG + chr8_2:61021424-61021443 None:intergenic 25.0%
! TTAAGACTTGTAAAGTAGTT+AGG - chr8_2:61021439-61021458 Msa1259390:intron 25.0%
AAGAACAAGAGATTTGAGAA+CGG + chr8_2:61018656-61018675 None:intergenic 30.0%
!!! TCTTCTGTTTTTACTTTCTG+TGG - chr8_2:61018813-61018832 Msa1259390:three_prime_UTR 30.0%
AAGTGAAAAAGGTGAAAACA+CGG + chr8_2:61018852-61018871 None:intergenic 30.0%
! ATAGAGATTTCGTAGGTTTT+GGG - chr8_2:61019091-61019110 Msa1259390:three_prime_UTR 30.0%
AAAGTTGTGAGAATGTTGTT+TGG - chr8_2:61019152-61019171 Msa1259390:three_prime_UTR 30.0%
!! GTTCATTTTTGCCTTTTCTT+TGG - chr8_2:61019584-61019603 Msa1259390:intron 30.0%
AGAAAAAATCGCCAAAGAAA+AGG + chr8_2:61019598-61019617 None:intergenic 30.0%
!!! CTCTGTTTTGATGTTGTTAT+TGG - chr8_2:61019616-61019635 Msa1259390:intron 30.0%
AGATGAAATTTCAACATGTG+TGG + chr8_2:61019648-61019667 None:intergenic 30.0%
! TTTCAATGCATCTGAAGTTA+TGG - chr8_2:61019745-61019764 Msa1259390:intron 30.0%
! TTCAATGCATCTGAAGTTAT+GGG - chr8_2:61019746-61019765 Msa1259390:intron 30.0%
ATGGGTATAAAAAAGAAGGA+TGG - chr8_2:61019764-61019783 Msa1259390:intron 30.0%
GAAGAAAAGACCCTAAAATT+TGG - chr8_2:61019839-61019858 Msa1259390:intron 30.0%
! GAATCAAACGCCAAATTTTA+GGG + chr8_2:61019852-61019871 None:intergenic 30.0%
GTTAACTAAAGCTTAGTTTC+CGG - chr8_2:61020011-61020030 Msa1259390:intron 30.0%
ACATAAACATCTTGCATAAC+CGG + chr8_2:61020033-61020052 None:intergenic 30.0%
AAATGATTGAAAACACACCA+AGG + chr8_2:61020224-61020243 None:intergenic 30.0%
ATATCAGTACCTAATAACAC+TGG + chr8_2:61020249-61020268 None:intergenic 30.0%
AAACAATAACTCCTAACTCA+TGG + chr8_2:61020405-61020424 None:intergenic 30.0%
GAGTTATTGTTTATAAGCTG+TGG - chr8_2:61020413-61020432 Msa1259390:five_prime_UTR 30.0%
TTATTAGGAATGACTGTCTT+TGG - chr8_2:61020468-61020487 Msa1259390:five_prime_UTR 30.0%
AAAATATCAGGGAAACAATC+TGG + chr8_2:61020929-61020948 None:intergenic 30.0%
TCAATTTGAGAAAAAGAGAC+TGG + chr8_2:61021158-61021177 None:intergenic 30.0%
!! CAAATTATGAGTTGGTGTAT+TGG + chr8_2:61021352-61021371 None:intergenic 30.0%
TTTCAATCGGTGAAGAATGA+GGG + chr8_2:61018608-61018627 None:intergenic 35.0%
GTCTTTCATAGAGATTTCGT+AGG - chr8_2:61019084-61019103 Msa1259390:three_prime_UTR 35.0%
! CATAGAGATTTCGTAGGTTT+TGG - chr8_2:61019090-61019109 Msa1259390:three_prime_UTR 35.0%
TAAACCAGGAAAAGAATGCA+GGG + chr8_2:61019426-61019445 None:intergenic 35.0%
TTAAACCAGGAAAAGAATGC+AGG + chr8_2:61019427-61019446 None:intergenic 35.0%
TTTCCTGGTTTAAATGCTGT+AGG - chr8_2:61019434-61019453 Msa1259390:intron 35.0%
ATTCCTACAGCATTTAAACC+AGG + chr8_2:61019440-61019459 None:intergenic 35.0%
AGACAAATTTGAGTCTGCTT+TGG - chr8_2:61019480-61019499 Msa1259390:five_prime_UTR 35.0%
GACAAATTTGAGTCTGCTTT+GGG - chr8_2:61019481-61019500 Msa1259390:five_prime_UTR 35.0%
AAGCAATTGTTCTGTCATCA+GGG + chr8_2:61019715-61019734 None:intergenic 35.0%
AAAGCAATTGTTCTGTCATC+AGG + chr8_2:61019716-61019735 None:intergenic 35.0%
TTTCTCTCTAGACAACTCTT+GGG + chr8_2:61019811-61019830 None:intergenic 35.0%
! TTTTCTCTCTAGACAACTCT+TGG + chr8_2:61019812-61019831 None:intergenic 35.0%
! CGAATCAAACGCCAAATTTT+AGG + chr8_2:61019853-61019872 None:intergenic 35.0%
TTGTGTAAGTTTAGTTGCCT+TGG - chr8_2:61020204-61020223 Msa1259390:intron 35.0%
!! AGGTACTGATATTGTTCTAG+TGG - chr8_2:61020257-61020276 Msa1259390:five_prime_UTR 35.0%
TTCTAGTGGTCAAATCAAGT+TGG - chr8_2:61020271-61020290 Msa1259390:five_prime_UTR 35.0%
! TCCACTCTTATTTGTAGGAA+AGG - chr8_2:61020310-61020329 Msa1259390:five_prime_UTR 35.0%
TCCTTTCCTACAAATAAGAG+TGG + chr8_2:61020314-61020333 None:intergenic 35.0%
TGTGATGTGACAATAGAGAA+AGG + chr8_2:61020561-61020580 None:intergenic 35.0%
TTCTGTAATGATGTAGGTAC+CGG - chr8_2:61020823-61020842 Msa1259390:intron 35.0%
TACTTAACTGTGTGTAACTG+CGG + chr8_2:61020877-61020896 None:intergenic 35.0%
!!! CAAGTTGTTTTGATCTGGTT+TGG + chr8_2:61020988-61021007 None:intergenic 35.0%
AACCAGATCAAAACAACTTG+AGG - chr8_2:61020988-61021007 Msa1259390:intron 35.0%
!! CATGAATGATGAGTTGATGT+CGG - chr8_2:61021023-61021042 Msa1259390:intron 35.0%
! GAGGTATTCAATCTACGTAT+GGG + chr8_2:61021185-61021204 None:intergenic 35.0%
!! AGAGGTATTCAATCTACGTA+TGG + chr8_2:61021186-61021205 None:intergenic 35.0%
TTCAATCGGTGAAGAATGAG+GGG + chr8_2:61018607-61018626 None:intergenic 40.0%
GTTTCAATCGGTGAAGAATG+AGG + chr8_2:61018609-61018628 None:intergenic 40.0%
! GGATCTGGTTCAGTTTCAAT+CGG + chr8_2:61018621-61018640 None:intergenic 40.0%
CATGAACAGTGTTGCGATTT+GGG + chr8_2:61018686-61018705 None:intergenic 40.0%
CAAATCGCAACACTGTTCAT+GGG - chr8_2:61018685-61018704 Msa1259390:three_prime_UTR 40.0%
TGGGATTTAAGAGAGGGATT+TGG + chr8_2:61018725-61018744 None:intergenic 40.0%
CACTGTTCATCAGGTTCAAA+GGG - chr8_2:61018746-61018765 Msa1259390:three_prime_UTR 40.0%
CTGTAGGAATGCTAAATACG+AGG - chr8_2:61019450-61019469 Msa1259390:CDS 40.0%
! AATTTGGCGTTTGATTCGCT+AGG - chr8_2:61019855-61019874 Msa1259390:intron 40.0%
!! GTGCAACTTTCTGTTTCTGA+AGG - chr8_2:61019930-61019949 Msa1259390:intron 40.0%
TGACAATAGAGAAAGGCTTG+AGG + chr8_2:61020554-61020573 None:intergenic 40.0%
! TCTTTGGTTCACAATGGAAG+AGG - chr8_2:61020677-61020696 Msa1259390:five_prime_UTR 40.0%
!! TAATGATGTAGGTACCGGAT+TGG - chr8_2:61020828-61020847 Msa1259390:intron 40.0%
!! AATGATGTAGGTACCGGATT+GGG - chr8_2:61020829-61020848 Msa1259390:intron 40.0%
!! TTGGGATTTGCTGCTGTAAA+AGG - chr8_2:61020847-61020866 Msa1259390:intron 40.0%
ACTTAACTGTGTGTAACTGC+GGG + chr8_2:61020876-61020895 None:intergenic 40.0%
AAACAATCTGGTACGTTGGA+TGG + chr8_2:61020917-61020936 None:intergenic 40.0%
!!! GACCTCAAGTTGTTTTGATC+TGG + chr8_2:61020993-61021012 None:intergenic 40.0%
ACAACTTGAGGTCACTCTAT+CGG - chr8_2:61021000-61021019 Msa1259390:intron 40.0%
GAGATTGAATCCTTGTCCAT+CGG - chr8_2:61021066-61021085 Msa1259390:intron 40.0%
GAGAAAAAGAGACTGGTAGA+GGG + chr8_2:61021151-61021170 None:intergenic 40.0%
TGAGAAAAAGAGACTGGTAG+AGG + chr8_2:61021152-61021171 None:intergenic 40.0%
! GCGATGTTGAATCTCTCTTT+TGG - chr8_2:61021232-61021251 Msa1259390:intron 40.0%
AGAATTCGACAGAGACAAAG+AGG - chr8_2:61021322-61021341 Msa1259390:intron 40.0%
TGAGAACGGTGAAGAACAGA+GGG + chr8_2:61018642-61018661 None:intergenic 45.0%
TTGAGAACGGTGAAGAACAG+AGG + chr8_2:61018643-61018662 None:intergenic 45.0%
CCATGAACAGTGTTGCGATT+TGG + chr8_2:61018687-61018706 None:intergenic 45.0%
CCAAATCGCAACACTGTTCA+TGG - chr8_2:61018684-61018703 Msa1259390:three_prime_UTR 45.0%
! CAGTGCTGGGATTTAAGAGA+GGG + chr8_2:61018731-61018750 None:intergenic 45.0%
! ACAGTGCTGGGATTTAAGAG+AGG + chr8_2:61018732-61018751 None:intergenic 45.0%
AAATCCCAGCACTGTTCATC+AGG - chr8_2:61018737-61018756 Msa1259390:three_prime_UTR 45.0%
TGAACCTGATGAACAGTGCT+GGG + chr8_2:61018744-61018763 None:intergenic 45.0%
TTGAACCTGATGAACAGTGC+TGG + chr8_2:61018745-61018764 None:intergenic 45.0%
GCACTGTTCATCAGGTTCAA+AGG - chr8_2:61018745-61018764 Msa1259390:three_prime_UTR 45.0%
!!! TCGTAGGTTTTGGGAGTGTT+TGG - chr8_2:61019100-61019119 Msa1259390:three_prime_UTR 45.0%
! CTTACCCTGCATTCTTTTCC+TGG - chr8_2:61019419-61019438 Msa1259390:intron 45.0%
AAAGAAGGATGGTGTTCCAG+AGG - chr8_2:61019775-61019794 Msa1259390:intron 45.0%
!! TTGATTCGCTAGGCTTTGGA+TGG - chr8_2:61019865-61019884 Msa1259390:intron 45.0%
TTTGGATGGCATGGAGAATG+AGG - chr8_2:61019879-61019898 Msa1259390:intron 45.0%
CAGAAGCTTTGCCATGAGTT+AGG - chr8_2:61020391-61020410 Msa1259390:five_prime_UTR 45.0%
GAGAAAGGCTTGAGGATCTA+AGG + chr8_2:61020546-61020565 None:intergenic 45.0%
GTTCACAATGGAAGAGGACA+CGG - chr8_2:61020683-61020702 Msa1259390:five_prime_UTR 45.0%
TACCTCTTCCACTGGTAACA+TGG + chr8_2:61020713-61020732 None:intergenic 45.0%
TCACATAGTACCTCTTCCAC+TGG + chr8_2:61020721-61020740 None:intergenic 45.0%
TACAGCAGCAAATCCCAATC+CGG + chr8_2:61020845-61020864 None:intergenic 45.0%
!! GTTGGATGGCTTTAATGGTC+TGG + chr8_2:61020903-61020922 None:intergenic 45.0%
!! GGTACGTTGGATGGCTTTAA+TGG + chr8_2:61020908-61020927 None:intergenic 45.0%
AGGGAAACAATCTGGTACGT+TGG + chr8_2:61020921-61020940 None:intergenic 45.0%
!! AATGGCATGAGAAGCACTCA+TGG - chr8_2:61021501-61021520 Msa1259390:five_prime_UTR 45.0%
!!! TTTATGTTTTTTTTTTATTT+TGG - chr8_2:61020046-61020065 Msa1259390:intron 5.0%
!!! TTATGTTTTTTTTTTATTTT+GGG - chr8_2:61020047-61020066 Msa1259390:intron 5.0%
GGGAACAAAACCAGAACCCA+AGG - chr8_2:61019501-61019520 Msa1259390:five_prime_UTR 50.0%
! CTTGCTACTGCTGCTTCCTT+GGG + chr8_2:61019520-61019539 None:intergenic 50.0%
! ACTTGCTACTGCTGCTTCCT+TGG + chr8_2:61019521-61019540 None:intergenic 50.0%
GGATGGTGTTCCAGAGGAAA+AGG - chr8_2:61019781-61019800 Msa1259390:intron 50.0%
! CTTGGGTCTACCTTTTCCTC+TGG + chr8_2:61019794-61019813 None:intergenic 50.0%
! GCGTTTGATTCGCTAGGCTT+TGG - chr8_2:61019861-61019880 Msa1259390:intron 50.0%
GAATGAGGCAAATTCGCGCA+CGG - chr8_2:61019894-61019913 Msa1259390:intron 50.0%
TGCCATGTTACCAGTGGAAG+AGG - chr8_2:61020708-61020727 Msa1259390:intron 50.0%
CGGTGAAGAACAGAGGGATC+TGG + chr8_2:61018636-61018655 None:intergenic 55.0%
! ACTGCTGCTTCCTTGGGTTC+TGG + chr8_2:61019514-61019533 None:intergenic 55.0%
!! TCGCTAGGCTTTGGATGGCA+TGG - chr8_2:61019870-61019889 Msa1259390:intron 55.0%
ACGGCTTGCCATGTTACCAG+TGG - chr8_2:61020702-61020721 Msa1259390:intron 55.0%
AGCGACGTCGTTTGCACTCT+TGG + chr8_2:61020965-61020984 None:intergenic 55.0%
GACTGGTAGAGGGACGAAGA+AGG + chr8_2:61021141-61021160 None:intergenic 55.0%
!! GAGTTGATGTCGGCACGACG+AGG - chr8_2:61021033-61021052 Msa1259390:intron 60.0%
GTCGGCACGACGAGGTCATA+TGG - chr8_2:61021041-61021060 Msa1259390:intron 60.0%
CGACCTCTCCTTCTCGACGA+CGG + chr8_2:61021099-61021118 None:intergenic 60.0%
CGTCGAGAAGGAGAGGTCGT+CGG - chr8_2:61021100-61021119 Msa1259390:intron 60.0%
CGGCGCAACACCGATGGACA+AGG + chr8_2:61021079-61021098 None:intergenic 65.0%
GTGTTGCGCCGTCGTCGAGA+AGG - chr8_2:61021088-61021107 Msa1259390:intron 65.0%
CGCGCACGGAACGCTGCAAC+AGG - chr8_2:61019908-61019927 Msa1259390:intron 70.0%
CGACGACGGCGCAACACCGA+TGG + chr8_2:61021085-61021104 None:intergenic 70.0%
GCGCCGTCGTCGAGAAGGAG+AGG - chr8_2:61021093-61021112 Msa1259390:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 61018598 61021566 61018598 ID=Msa1259390;Name=Msa1259390
chr8_2 mRNA 61018598 61021566 61018598 ID=Msa1259390-mRNA-1;Parent=Msa1259390;Name=Msa1259390-mRNA-1;_AED=0.21;_eAED=0.21;_QI=676|1|1|1|0.25|0.4|5|669|32
chr8_2 exon 61018598 61019325 61018598 ID=Msa1259390-mRNA-1:exon:19202;Parent=Msa1259390-mRNA-1
chr8_2 exon 61019435 61019540 61019435 ID=Msa1259390-mRNA-1:exon:19201;Parent=Msa1259390-mRNA-1
chr8_2 exon 61020235 61020708 61020235 ID=Msa1259390-mRNA-1:exon:19200;Parent=Msa1259390-mRNA-1
chr8_2 exon 61021379 61021405 61021379 ID=Msa1259390-mRNA-1:exon:19199;Parent=Msa1259390-mRNA-1
chr8_2 exon 61021458 61021566 61021458 ID=Msa1259390-mRNA-1:exon:19198;Parent=Msa1259390-mRNA-1
chr8_2 five_prime_UTR 61021458 61021566 61021458 ID=Msa1259390-mRNA-1:five_prime_utr;Parent=Msa1259390-mRNA-1
chr8_2 five_prime_UTR 61021379 61021405 61021379 ID=Msa1259390-mRNA-1:five_prime_utr;Parent=Msa1259390-mRNA-1
chr8_2 five_prime_UTR 61020235 61020708 61020235 ID=Msa1259390-mRNA-1:five_prime_utr;Parent=Msa1259390-mRNA-1
chr8_2 five_prime_UTR 61019475 61019540 61019475 ID=Msa1259390-mRNA-1:five_prime_utr;Parent=Msa1259390-mRNA-1
chr8_2 CDS 61019435 61019474 61019435 ID=Msa1259390-mRNA-1:cds;Parent=Msa1259390-mRNA-1
chr8_2 CDS 61019267 61019325 61019267 ID=Msa1259390-mRNA-1:cds;Parent=Msa1259390-mRNA-1
chr8_2 three_prime_UTR 61018598 61019266 61018598 ID=Msa1259390-mRNA-1:three_prime_utr;Parent=Msa1259390-mRNA-1
Gene Sequence

>Msa1259390

ATGGAAGAGGACACGGCTTGCCATGTTACCAGTGGAAGAGGTACCGGATTGGGATTTGCTGCTGTAAAAGGAATCCCGCAGTTACACACAGTTAAGTAA
Protein sequence

>Msa1259390

MEEDTACHVTSGRGTGLGFAAVKGIPQLHTVK*