AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0315200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATGACATAAATCAAAAT+TGG 0.154406 2_4:-11396436 None:intergenic
TTGGGATTTGCTGCTGTAAA+AGG 0.245335 2_4:+11396014 Msa0315200:CDS
GAAGAAAAGACCCTAAAATT+TGG 0.284411 2_4:+11395005 Msa0315200:five_prime_UTR
ACTGCTGCTTCCTTGGGTTC+TGG 0.294667 2_4:-11394677 None:intergenic
ACTTGCTACTGCTGCTTCCT+TGG 0.295570 2_4:-11394684 None:intergenic
TGATGACATAAATCAAAATT+GGG 0.308819 2_4:-11396435 None:intergenic
TTTCAATGCATCTGAAGTTA+TGG 0.314410 2_4:+11394911 Msa0315200:five_prime_UTR
AGCGACGTCGTTTGCACTCT+TGG 0.322976 2_4:-11396129 None:intergenic
TAACAGTTATCTATATAAAA+AGG 0.323600 2_4:+11395812 Msa0315200:five_prime_UTR
AAAGCAATTGTTCTGTCATC+AGG 0.325832 2_4:-11394879 None:intergenic
GGTACGTTGGATGGCTTTAA+TGG 0.329174 2_4:-11396072 None:intergenic
GACAAATTTGAGTCTGCTTT+GGG 0.329614 2_4:+11394647 Msa0315200:five_prime_UTR
TTAAGACTTGTAAAGTAGTT+AGG 0.331314 2_4:+11396606 Msa0315200:three_prime_UTR
GCGATGTTGAATCTCTCTTT+TGG 0.347039 2_4:+11396399 Msa0315200:three_prime_UTR
CGCGCACGGAACGCTGCAAC+AGG 0.350992 2_4:+11395074 Msa0315200:five_prime_UTR
AGACAAATTTGAGTCTGCTT+TGG 0.352947 2_4:+11394646 Msa0315200:five_prime_UTR
GAATGCAGGGTAAGAATAAC+AGG 0.370892 2_4:-11394576 None:intergenic
CTTGCTACTGCTGCTTCCTT+GGG 0.371354 2_4:-11394683 None:intergenic
CAAATTATGAGTTGGTGTAT+TGG 0.382778 2_4:-11396516 None:intergenic
GCGTTTGATTCGCTAGGCTT+TGG 0.384574 2_4:+11395027 Msa0315200:five_prime_UTR
GTTGGATGGCTTTAATGGTC+TGG 0.390968 2_4:-11396067 None:intergenic
TTGATTTATGTCATCAAAGT+TGG 0.391346 2_4:+11396442 Msa0315200:three_prime_UTR
TTCAATGCATCTGAAGTTAT+GGG 0.417554 2_4:+11394912 Msa0315200:five_prime_UTR
CATAATTTGAAGATATTACA+TGG 0.430630 2_4:+11396530 Msa0315200:three_prime_UTR
AAAATATCAGGGAAACAATC+TGG 0.446965 2_4:-11396093 None:intergenic
TAAAACAGTGAATTTAAAAG+AGG 0.452932 2_4:-11396368 None:intergenic
GGATGGTGTTCCAGAGGAAA+AGG 0.461035 2_4:+11394947 Msa0315200:five_prime_UTR
AATTCTGTCAAGAGAAATAA+AGG 0.467296 2_4:-11396472 None:intergenic
GTCGGCACGACGAGGTCATA+TGG 0.480658 2_4:+11396208 Msa0315200:three_prime_UTR
AGAGGTATTCAATCTACGTA+TGG 0.495214 2_4:-11396350 None:intergenic
AATGATGTAGGTACCGGATT+GGG 0.499273 2_4:+11395996 Msa0315200:intron
TTTCTCTCTAGACAACTCTT+GGG 0.503761 2_4:-11394974 None:intergenic
TCGCTAGGCTTTGGATGGCA+TGG 0.505322 2_4:+11395036 Msa0315200:five_prime_UTR
CGACGACGGCGCAACACTGA+TGG 0.506127 2_4:-11396249 None:intergenic
TTGATTCGCTAGGCTTTGGA+TGG 0.506861 2_4:+11395031 Msa0315200:five_prime_UTR
TAATGATGTAGGTACCGGAT+TGG 0.513302 2_4:+11395995 Msa0315200:intron
TGCCATGTTACCAGTGGAAG+AGG 0.519937 2_4:+11395875 Msa0315200:CDS
AATGGCATGAGAAGCACTCA+TGG 0.522645 2_4:+11396668 Msa0315200:three_prime_UTR
TACAGCAGCAAATCCCAATC+CGG 0.525664 2_4:-11396009 None:intergenic
TTAAACCAGGAAAAGAATGC+AGG 0.527198 2_4:-11394590 None:intergenic
TCTTTGGTTCACAATGGAAG+AGG 0.528030 2_4:+11395844 Msa0315200:exon
AGGGAAACAATCTGGTACGT+TGG 0.537244 2_4:-11396085 None:intergenic
TTTGGATGGCATGGAGAATG+AGG 0.537359 2_4:+11395045 Msa0315200:five_prime_UTR
GACTGGTAGAGGGACGAAGA+AGG 0.539140 2_4:-11396305 None:intergenic
AAACAATCTGGTACGTTGGA+TGG 0.543310 2_4:-11396081 None:intergenic
ACTTAACTGTGTGTAACTGC+GGG 0.549402 2_4:-11396040 None:intergenic
AATATCTTCAAATTATGAGT+TGG 0.553846 2_4:-11396524 None:intergenic
TTTCCTGGTTTAAATGCTGT+AGG 0.558177 2_4:+11394600 Msa0315200:five_prime_UTR
TACTTAACTGTGTGTAACTG+CGG 0.560947 2_4:-11396041 None:intergenic
ACAACTTGAGGTCACTCTAT+CGG 0.561540 2_4:+11396167 Msa0315200:three_prime_UTR
TCACATAGTACCTCTTCCAC+TGG 0.564675 2_4:-11395885 None:intergenic
AAGCAATTGTTCTGTCATCA+GGG 0.565976 2_4:-11394878 None:intergenic
TACCTCTTCCACTGGTAACA+TGG 0.566570 2_4:-11395877 None:intergenic
GATGACATAAATCAAAATTG+GGG 0.567982 2_4:-11396434 None:intergenic
GAGGTATTCAATCTACGTAT+GGG 0.587598 2_4:-11396349 None:intergenic
GTGTTGCGCCGTCGTCGAGA+AGG 0.591269 2_4:+11396255 Msa0315200:three_prime_UTR
ATTATTTCTTTGGTTCACAA+TGG 0.591280 2_4:+11395838 Msa0315200:exon
AGAATTCGACAGAGACAAAG+AGG 0.596231 2_4:+11396489 Msa0315200:three_prime_UTR
GCGCCGTCGTCGAGAAGGAG+AGG 0.596832 2_4:+11396260 Msa0315200:three_prime_UTR
GTTCACAATGGAAGAGGACA+CGG 0.598072 2_4:+11395850 Msa0315200:exon
ATTCCTACAGCATTTAAACC+AGG 0.598918 2_4:-11394603 None:intergenic
CGGCGCAACACTGATGGACA+AGG 0.601039 2_4:-11396243 None:intergenic
AGATGAAATTTCAACATGTG+TGG 0.606032 2_4:-11394811 None:intergenic
TAAACCAGGAAAAGAATGCA+GGG 0.607110 2_4:-11394589 None:intergenic
AACCAGATCAAAACAACTTG+AGG 0.607973 2_4:+11396155 Msa0315200:three_prime_UTR
GGGAACAAAACCAGAACCCA+AGG 0.621328 2_4:+11394667 Msa0315200:five_prime_UTR
AATTTGGCGTTTGATTCGCT+AGG 0.629334 2_4:+11395021 Msa0315200:five_prime_UTR
AAAGAAGGATGGTGTTCCAG+AGG 0.634111 2_4:+11394941 Msa0315200:five_prime_UTR
CTGTAGGAATGCTAAATACG+AGG 0.634576 2_4:+11394616 Msa0315200:five_prime_UTR
CGTCGAGAAGGAGAGGTCGT+CGG 0.647129 2_4:+11396267 Msa0315200:three_prime_UTR
GAATGAGGCAAATTCGCGCA+CGG 0.657463 2_4:+11395060 Msa0315200:five_prime_UTR
CATGAATGATGAGTTGATGT+CGG 0.665319 2_4:+11396190 Msa0315200:three_prime_UTR
ACGGCTTGCCATGTTACCAG+TGG 0.669509 2_4:+11395869 Msa0315200:CDS
CGACCTCTCCTTCTCGACGA+CGG 0.677608 2_4:-11396263 None:intergenic
GAGTTGATGTCGGCACGACG+AGG 0.703590 2_4:+11396200 Msa0315200:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTTTGAATCTTAAAAT+AGG + chr2_4:11395286-11395305 Msa0315200:intron 10.0%
!!! TATATTTTGAATCTTAAAAT+AGG + chr2_4:11395286-11395305 Msa0315200:intron 10.0%
!!! TTGTAGTTTTAATATCATAA+TGG - chr2_4:11394539-11394558 None:intergenic 15.0%
!! ACTAAATCAATTATGTTATT+AGG + chr2_4:11395620-11395639 Msa0315200:intron 15.0%
!!! AAATTAAATTTGATTTAAGC+AGG + chr2_4:11395661-11395680 Msa0315200:intron 15.0%
!!! AATTAAATTTGATTTAAGCA+GGG + chr2_4:11395662-11395681 Msa0315200:intron 15.0%
!! TAACAGTTATCTATATAAAA+AGG + chr2_4:11395812-11395831 Msa0315200:five_prime_UTR 15.0%
!! AGTAAAAACATAAAATATCA+GGG - chr2_4:11396107-11396126 None:intergenic 15.0%
!!! TTGTAGTTTTAATATCATAA+TGG - chr2_4:11394539-11394558 None:intergenic 15.0%
!! ACTAAATCAATTATGTTATT+AGG + chr2_4:11395620-11395639 Msa0315200:intron 15.0%
!!! AAATTAAATTTGATTTAAGC+AGG + chr2_4:11395661-11395680 Msa0315200:intron 15.0%
!!! AATTAAATTTGATTTAAGCA+GGG + chr2_4:11395662-11395681 Msa0315200:intron 15.0%
!! TAACAGTTATCTATATAAAA+AGG + chr2_4:11395812-11395831 Msa0315200:five_prime_UTR 15.0%
!! AGTAAAAACATAAAATATCA+GGG - chr2_4:11396107-11396126 None:intergenic 15.0%
!! AAATATCTTTACAATAGTGA+TGG + chr2_4:11395255-11395274 Msa0315200:intron 20.0%
!! AAAATGCTATATGTTCATAT+AGG + chr2_4:11395769-11395788 Msa0315200:intron 20.0%
!!! AAAAAGGTTCATTATTTCTT+TGG + chr2_4:11395828-11395847 Msa0315200:five_prime_UTR 20.0%
!! ATTCCATTAAAATCAACAAA+AGG - chr2_4:11395926-11395945 None:intergenic 20.0%
!!! ATTTATTTCTGTAATGATGT+AGG + chr2_4:11395984-11396003 Msa0315200:intron 20.0%
!! CAGTAAAAACATAAAATATC+AGG - chr2_4:11396108-11396127 None:intergenic 20.0%
!! TAAAACAGTGAATTTAAAAG+AGG - chr2_4:11396371-11396390 None:intergenic 20.0%
!! TGATGACATAAATCAAAATT+GGG - chr2_4:11396438-11396457 None:intergenic 20.0%
!!! TTGATGACATAAATCAAAAT+TGG - chr2_4:11396439-11396458 None:intergenic 20.0%
!! AATATCTTCAAATTATGAGT+TGG - chr2_4:11396527-11396546 None:intergenic 20.0%
!! CATAATTTGAAGATATTACA+TGG + chr2_4:11396530-11396549 Msa0315200:three_prime_UTR 20.0%
!!! TTAGTTTTGTATTACTGAAA+TGG + chr2_4:11396650-11396669 Msa0315200:three_prime_UTR 20.0%
!! AAATATCTTTACAATAGTGA+TGG + chr2_4:11395255-11395274 Msa0315200:intron 20.0%
!! AAAATGCTATATGTTCATAT+AGG + chr2_4:11395769-11395788 Msa0315200:intron 20.0%
!!! AAAAAGGTTCATTATTTCTT+TGG + chr2_4:11395828-11395847 Msa0315200:five_prime_UTR 20.0%
!! ATTCCATTAAAATCAACAAA+AGG - chr2_4:11395926-11395945 None:intergenic 20.0%
!!! ATTTATTTCTGTAATGATGT+AGG + chr2_4:11395984-11396003 Msa0315200:intron 20.0%
!! CAGTAAAAACATAAAATATC+AGG - chr2_4:11396108-11396127 None:intergenic 20.0%
!! TAAAACAGTGAATTTAAAAG+AGG - chr2_4:11396371-11396390 None:intergenic 20.0%
!! TGATGACATAAATCAAAATT+GGG - chr2_4:11396438-11396457 None:intergenic 20.0%
!!! TTGATGACATAAATCAAAAT+TGG - chr2_4:11396439-11396458 None:intergenic 20.0%
!! AATATCTTCAAATTATGAGT+TGG - chr2_4:11396527-11396546 None:intergenic 20.0%
!! CATAATTTGAAGATATTACA+TGG + chr2_4:11396530-11396549 Msa0315200:three_prime_UTR 20.0%
!!! TTAGTTTTGTATTACTGAAA+TGG + chr2_4:11396650-11396669 Msa0315200:three_prime_UTR 20.0%
! AGTTATGGGTATAAAAAAGA+AGG + chr2_4:11394926-11394945 Msa0315200:five_prime_UTR 25.0%
!! ATCATTTAACCAGTGTTATT+AGG + chr2_4:11395404-11395423 Msa0315200:intron 25.0%
!!! TTTTTTCCACTCTTATTTGT+AGG + chr2_4:11395472-11395491 Msa0315200:intron 25.0%
!! ATTATTTCTTTGGTTCACAA+TGG + chr2_4:11395838-11395857 Msa0315200:exon 25.0%
!!! TGTCCTTTTGTTGATTTTAA+TGG + chr2_4:11395920-11395939 Msa0315200:intron 25.0%
! GATGACATAAATCAAAATTG+GGG - chr2_4:11396437-11396456 None:intergenic 25.0%
! TTGATTTATGTCATCAAAGT+TGG + chr2_4:11396442-11396461 Msa0315200:three_prime_UTR 25.0%
! AATTCTGTCAAGAGAAATAA+AGG - chr2_4:11396475-11396494 None:intergenic 25.0%
! CTTAAAAAAATTCTGCACAA+TGG - chr2_4:11396591-11396610 None:intergenic 25.0%
! TTAAGACTTGTAAAGTAGTT+AGG + chr2_4:11396606-11396625 Msa0315200:three_prime_UTR 25.0%
! AGTTATGGGTATAAAAAAGA+AGG + chr2_4:11394926-11394945 Msa0315200:five_prime_UTR 25.0%
!! ATCATTTAACCAGTGTTATT+AGG + chr2_4:11395404-11395423 Msa0315200:intron 25.0%
!!! TTTTTTCCACTCTTATTTGT+AGG + chr2_4:11395472-11395491 Msa0315200:intron 25.0%
!! ATTATTTCTTTGGTTCACAA+TGG + chr2_4:11395838-11395857 Msa0315200:exon 25.0%
!!! TGTCCTTTTGTTGATTTTAA+TGG + chr2_4:11395920-11395939 Msa0315200:intron 25.0%
! GATGACATAAATCAAAATTG+GGG - chr2_4:11396437-11396456 None:intergenic 25.0%
! TTGATTTATGTCATCAAAGT+TGG + chr2_4:11396442-11396461 Msa0315200:three_prime_UTR 25.0%
! AATTCTGTCAAGAGAAATAA+AGG - chr2_4:11396475-11396494 None:intergenic 25.0%
! CTTAAAAAAATTCTGCACAA+TGG - chr2_4:11396591-11396610 None:intergenic 25.0%
! TTAAGACTTGTAAAGTAGTT+AGG + chr2_4:11396606-11396625 Msa0315200:three_prime_UTR 25.0%
!! GTTCATTTTTGCCTTTTCTT+TGG + chr2_4:11394750-11394769 Msa0315200:five_prime_UTR 30.0%
AGAAAAAATCGCCAAAGAAA+AGG - chr2_4:11394764-11394783 None:intergenic 30.0%
!!! CTCTGTTTTGATGTTGTTAT+TGG + chr2_4:11394782-11394801 Msa0315200:five_prime_UTR 30.0%
AGATGAAATTTCAACATGTG+TGG - chr2_4:11394814-11394833 None:intergenic 30.0%
! TTTCAATGCATCTGAAGTTA+TGG + chr2_4:11394911-11394930 Msa0315200:five_prime_UTR 30.0%
! TTCAATGCATCTGAAGTTAT+GGG + chr2_4:11394912-11394931 Msa0315200:five_prime_UTR 30.0%
ATGGGTATAAAAAAGAAGGA+TGG + chr2_4:11394930-11394949 Msa0315200:five_prime_UTR 30.0%
GAAGAAAAGACCCTAAAATT+TGG + chr2_4:11395005-11395024 Msa0315200:five_prime_UTR 30.0%
! GAATCAAACGCCAAATTTTA+GGG - chr2_4:11395018-11395037 None:intergenic 30.0%
GTTAACTAAAGCTTAGTTTC+CGG + chr2_4:11395177-11395196 Msa0315200:intron 30.0%
ACATAAACATCTTGCATAAC+CGG - chr2_4:11395199-11395218 None:intergenic 30.0%
AAATGATTGAAAACACACCA+AGG - chr2_4:11395391-11395410 None:intergenic 30.0%
ATATCAGTACCTAATAACAC+TGG - chr2_4:11395416-11395435 None:intergenic 30.0%
AAACAATAACTCCTAACTCA+TGG - chr2_4:11395572-11395591 None:intergenic 30.0%
GAGTTATTGTTTATAAGCTG+TGG + chr2_4:11395580-11395599 Msa0315200:intron 30.0%
TTATTAGGAATGACTGTCTT+TGG + chr2_4:11395635-11395654 Msa0315200:intron 30.0%
AAAATATCAGGGAAACAATC+TGG - chr2_4:11396096-11396115 None:intergenic 30.0%
TCAATTTGAGAAAAAGAGAC+TGG - chr2_4:11396325-11396344 None:intergenic 30.0%
!! CAAATTATGAGTTGGTGTAT+TGG - chr2_4:11396519-11396538 None:intergenic 30.0%
!! GTTCATTTTTGCCTTTTCTT+TGG + chr2_4:11394750-11394769 Msa0315200:five_prime_UTR 30.0%
AGAAAAAATCGCCAAAGAAA+AGG - chr2_4:11394764-11394783 None:intergenic 30.0%
!!! CTCTGTTTTGATGTTGTTAT+TGG + chr2_4:11394782-11394801 Msa0315200:five_prime_UTR 30.0%
AGATGAAATTTCAACATGTG+TGG - chr2_4:11394814-11394833 None:intergenic 30.0%
! TTTCAATGCATCTGAAGTTA+TGG + chr2_4:11394911-11394930 Msa0315200:five_prime_UTR 30.0%
! TTCAATGCATCTGAAGTTAT+GGG + chr2_4:11394912-11394931 Msa0315200:five_prime_UTR 30.0%
ATGGGTATAAAAAAGAAGGA+TGG + chr2_4:11394930-11394949 Msa0315200:five_prime_UTR 30.0%
GAAGAAAAGACCCTAAAATT+TGG + chr2_4:11395005-11395024 Msa0315200:five_prime_UTR 30.0%
! GAATCAAACGCCAAATTTTA+GGG - chr2_4:11395018-11395037 None:intergenic 30.0%
GTTAACTAAAGCTTAGTTTC+CGG + chr2_4:11395177-11395196 Msa0315200:intron 30.0%
ACATAAACATCTTGCATAAC+CGG - chr2_4:11395199-11395218 None:intergenic 30.0%
AAATGATTGAAAACACACCA+AGG - chr2_4:11395391-11395410 None:intergenic 30.0%
ATATCAGTACCTAATAACAC+TGG - chr2_4:11395416-11395435 None:intergenic 30.0%
AAACAATAACTCCTAACTCA+TGG - chr2_4:11395572-11395591 None:intergenic 30.0%
GAGTTATTGTTTATAAGCTG+TGG + chr2_4:11395580-11395599 Msa0315200:intron 30.0%
TTATTAGGAATGACTGTCTT+TGG + chr2_4:11395635-11395654 Msa0315200:intron 30.0%
AAAATATCAGGGAAACAATC+TGG - chr2_4:11396096-11396115 None:intergenic 30.0%
TCAATTTGAGAAAAAGAGAC+TGG - chr2_4:11396325-11396344 None:intergenic 30.0%
!! CAAATTATGAGTTGGTGTAT+TGG - chr2_4:11396519-11396538 None:intergenic 30.0%
TAAACCAGGAAAAGAATGCA+GGG - chr2_4:11394592-11394611 None:intergenic 35.0%
TTAAACCAGGAAAAGAATGC+AGG - chr2_4:11394593-11394612 None:intergenic 35.0%
TTTCCTGGTTTAAATGCTGT+AGG + chr2_4:11394600-11394619 Msa0315200:five_prime_UTR 35.0%
ATTCCTACAGCATTTAAACC+AGG - chr2_4:11394606-11394625 None:intergenic 35.0%
AGACAAATTTGAGTCTGCTT+TGG + chr2_4:11394646-11394665 Msa0315200:five_prime_UTR 35.0%
GACAAATTTGAGTCTGCTTT+GGG + chr2_4:11394647-11394666 Msa0315200:five_prime_UTR 35.0%
AAGCAATTGTTCTGTCATCA+GGG - chr2_4:11394881-11394900 None:intergenic 35.0%
AAAGCAATTGTTCTGTCATC+AGG - chr2_4:11394882-11394901 None:intergenic 35.0%
TTTCTCTCTAGACAACTCTT+GGG - chr2_4:11394977-11394996 None:intergenic 35.0%
! TTTTCTCTCTAGACAACTCT+TGG - chr2_4:11394978-11394997 None:intergenic 35.0%
! CGAATCAAACGCCAAATTTT+AGG - chr2_4:11395019-11395038 None:intergenic 35.0%
TTGTGTAAGTTTAGTTGCCT+TGG + chr2_4:11395371-11395390 Msa0315200:intron 35.0%
!! AGGTACTGATATTGTTCTAG+TGG + chr2_4:11395424-11395443 Msa0315200:intron 35.0%
TTCTAGTGGTCAAATCAAGT+TGG + chr2_4:11395438-11395457 Msa0315200:intron 35.0%
! TCCACTCTTATTTGTAGGAA+AGG + chr2_4:11395477-11395496 Msa0315200:intron 35.0%
TCCTTTCCTACAAATAAGAG+TGG - chr2_4:11395481-11395500 None:intergenic 35.0%
TGTGATGTGACAATAGAGAA+AGG - chr2_4:11395728-11395747 None:intergenic 35.0%
TTCTGTAATGATGTAGGTAC+CGG + chr2_4:11395990-11396009 Msa0315200:intron 35.0%
TACTTAACTGTGTGTAACTG+CGG - chr2_4:11396044-11396063 None:intergenic 35.0%
!!! CAAGTTGTTTTGATCTGGTT+TGG - chr2_4:11396155-11396174 None:intergenic 35.0%
AACCAGATCAAAACAACTTG+AGG + chr2_4:11396155-11396174 Msa0315200:three_prime_UTR 35.0%
!! CATGAATGATGAGTTGATGT+CGG + chr2_4:11396190-11396209 Msa0315200:three_prime_UTR 35.0%
! GAGGTATTCAATCTACGTAT+GGG - chr2_4:11396352-11396371 None:intergenic 35.0%
!! AGAGGTATTCAATCTACGTA+TGG - chr2_4:11396353-11396372 None:intergenic 35.0%
TAAACCAGGAAAAGAATGCA+GGG - chr2_4:11394592-11394611 None:intergenic 35.0%
TTAAACCAGGAAAAGAATGC+AGG - chr2_4:11394593-11394612 None:intergenic 35.0%
TTTCCTGGTTTAAATGCTGT+AGG + chr2_4:11394600-11394619 Msa0315200:five_prime_UTR 35.0%
ATTCCTACAGCATTTAAACC+AGG - chr2_4:11394606-11394625 None:intergenic 35.0%
AGACAAATTTGAGTCTGCTT+TGG + chr2_4:11394646-11394665 Msa0315200:five_prime_UTR 35.0%
GACAAATTTGAGTCTGCTTT+GGG + chr2_4:11394647-11394666 Msa0315200:five_prime_UTR 35.0%
AAGCAATTGTTCTGTCATCA+GGG - chr2_4:11394881-11394900 None:intergenic 35.0%
AAAGCAATTGTTCTGTCATC+AGG - chr2_4:11394882-11394901 None:intergenic 35.0%
TTTCTCTCTAGACAACTCTT+GGG - chr2_4:11394977-11394996 None:intergenic 35.0%
! TTTTCTCTCTAGACAACTCT+TGG - chr2_4:11394978-11394997 None:intergenic 35.0%
! CGAATCAAACGCCAAATTTT+AGG - chr2_4:11395019-11395038 None:intergenic 35.0%
TTGTGTAAGTTTAGTTGCCT+TGG + chr2_4:11395371-11395390 Msa0315200:intron 35.0%
!! AGGTACTGATATTGTTCTAG+TGG + chr2_4:11395424-11395443 Msa0315200:intron 35.0%
TTCTAGTGGTCAAATCAAGT+TGG + chr2_4:11395438-11395457 Msa0315200:intron 35.0%
! TCCACTCTTATTTGTAGGAA+AGG + chr2_4:11395477-11395496 Msa0315200:intron 35.0%
TCCTTTCCTACAAATAAGAG+TGG - chr2_4:11395481-11395500 None:intergenic 35.0%
TGTGATGTGACAATAGAGAA+AGG - chr2_4:11395728-11395747 None:intergenic 35.0%
TTCTGTAATGATGTAGGTAC+CGG + chr2_4:11395990-11396009 Msa0315200:intron 35.0%
TACTTAACTGTGTGTAACTG+CGG - chr2_4:11396044-11396063 None:intergenic 35.0%
!!! CAAGTTGTTTTGATCTGGTT+TGG - chr2_4:11396155-11396174 None:intergenic 35.0%
AACCAGATCAAAACAACTTG+AGG + chr2_4:11396155-11396174 Msa0315200:three_prime_UTR 35.0%
!! CATGAATGATGAGTTGATGT+CGG + chr2_4:11396190-11396209 Msa0315200:three_prime_UTR 35.0%
! GAGGTATTCAATCTACGTAT+GGG - chr2_4:11396352-11396371 None:intergenic 35.0%
!! AGAGGTATTCAATCTACGTA+TGG - chr2_4:11396353-11396372 None:intergenic 35.0%
GAATGCAGGGTAAGAATAAC+AGG - chr2_4:11394579-11394598 None:intergenic 40.0%
CTGTAGGAATGCTAAATACG+AGG + chr2_4:11394616-11394635 Msa0315200:five_prime_UTR 40.0%
! AATTTGGCGTTTGATTCGCT+AGG + chr2_4:11395021-11395040 Msa0315200:five_prime_UTR 40.0%
!! GTGCAACTTTCTGTTTCTGA+AGG + chr2_4:11395096-11395115 Msa0315200:intron 40.0%
TGACAATAGAGAAAGGCTTG+AGG - chr2_4:11395721-11395740 None:intergenic 40.0%
! TCTTTGGTTCACAATGGAAG+AGG + chr2_4:11395844-11395863 Msa0315200:exon 40.0%
!! TAATGATGTAGGTACCGGAT+TGG + chr2_4:11395995-11396014 Msa0315200:intron 40.0%
!! AATGATGTAGGTACCGGATT+GGG + chr2_4:11395996-11396015 Msa0315200:intron 40.0%
!! TTGGGATTTGCTGCTGTAAA+AGG + chr2_4:11396014-11396033 Msa0315200:CDS 40.0%
ACTTAACTGTGTGTAACTGC+GGG - chr2_4:11396043-11396062 None:intergenic 40.0%
AAACAATCTGGTACGTTGGA+TGG - chr2_4:11396084-11396103 None:intergenic 40.0%
!!! GACCTCAAGTTGTTTTGATC+TGG - chr2_4:11396160-11396179 None:intergenic 40.0%
ACAACTTGAGGTCACTCTAT+CGG + chr2_4:11396167-11396186 Msa0315200:three_prime_UTR 40.0%
GAGAAAAAGAGACTGGTAGA+GGG - chr2_4:11396318-11396337 None:intergenic 40.0%
TGAGAAAAAGAGACTGGTAG+AGG - chr2_4:11396319-11396338 None:intergenic 40.0%
! GCGATGTTGAATCTCTCTTT+TGG + chr2_4:11396399-11396418 Msa0315200:three_prime_UTR 40.0%
AGAATTCGACAGAGACAAAG+AGG + chr2_4:11396489-11396508 Msa0315200:three_prime_UTR 40.0%
GAATGCAGGGTAAGAATAAC+AGG - chr2_4:11394579-11394598 None:intergenic 40.0%
CTGTAGGAATGCTAAATACG+AGG + chr2_4:11394616-11394635 Msa0315200:five_prime_UTR 40.0%
! AATTTGGCGTTTGATTCGCT+AGG + chr2_4:11395021-11395040 Msa0315200:five_prime_UTR 40.0%
!! GTGCAACTTTCTGTTTCTGA+AGG + chr2_4:11395096-11395115 Msa0315200:intron 40.0%
TGACAATAGAGAAAGGCTTG+AGG - chr2_4:11395721-11395740 None:intergenic 40.0%
! TCTTTGGTTCACAATGGAAG+AGG + chr2_4:11395844-11395863 Msa0315200:exon 40.0%
!! TAATGATGTAGGTACCGGAT+TGG + chr2_4:11395995-11396014 Msa0315200:intron 40.0%
!! AATGATGTAGGTACCGGATT+GGG + chr2_4:11395996-11396015 Msa0315200:intron 40.0%
!! TTGGGATTTGCTGCTGTAAA+AGG + chr2_4:11396014-11396033 Msa0315200:CDS 40.0%
ACTTAACTGTGTGTAACTGC+GGG - chr2_4:11396043-11396062 None:intergenic 40.0%
AAACAATCTGGTACGTTGGA+TGG - chr2_4:11396084-11396103 None:intergenic 40.0%
!!! GACCTCAAGTTGTTTTGATC+TGG - chr2_4:11396160-11396179 None:intergenic 40.0%
ACAACTTGAGGTCACTCTAT+CGG + chr2_4:11396167-11396186 Msa0315200:three_prime_UTR 40.0%
GAGAAAAAGAGACTGGTAGA+GGG - chr2_4:11396318-11396337 None:intergenic 40.0%
TGAGAAAAAGAGACTGGTAG+AGG - chr2_4:11396319-11396338 None:intergenic 40.0%
! GCGATGTTGAATCTCTCTTT+TGG + chr2_4:11396399-11396418 Msa0315200:three_prime_UTR 40.0%
AGAATTCGACAGAGACAAAG+AGG + chr2_4:11396489-11396508 Msa0315200:three_prime_UTR 40.0%
! CTTACCCTGCATTCTTTTCC+TGG + chr2_4:11394585-11394604 Msa0315200:five_prime_UTR 45.0%
AAAGAAGGATGGTGTTCCAG+AGG + chr2_4:11394941-11394960 Msa0315200:five_prime_UTR 45.0%
!! TTGATTCGCTAGGCTTTGGA+TGG + chr2_4:11395031-11395050 Msa0315200:five_prime_UTR 45.0%
TTTGGATGGCATGGAGAATG+AGG + chr2_4:11395045-11395064 Msa0315200:five_prime_UTR 45.0%
CAGAAGCTTTGCCATGAGTT+AGG + chr2_4:11395558-11395577 Msa0315200:intron 45.0%
GAGAAAGGCTTGAGGATCTA+AGG - chr2_4:11395713-11395732 None:intergenic 45.0%
GTTCACAATGGAAGAGGACA+CGG + chr2_4:11395850-11395869 Msa0315200:exon 45.0%
TACCTCTTCCACTGGTAACA+TGG - chr2_4:11395880-11395899 None:intergenic 45.0%
TCACATAGTACCTCTTCCAC+TGG - chr2_4:11395888-11395907 None:intergenic 45.0%
TACAGCAGCAAATCCCAATC+CGG - chr2_4:11396012-11396031 None:intergenic 45.0%
!! GTTGGATGGCTTTAATGGTC+TGG - chr2_4:11396070-11396089 None:intergenic 45.0%
!! GGTACGTTGGATGGCTTTAA+TGG - chr2_4:11396075-11396094 None:intergenic 45.0%
AGGGAAACAATCTGGTACGT+TGG - chr2_4:11396088-11396107 None:intergenic 45.0%
!! AATGGCATGAGAAGCACTCA+TGG + chr2_4:11396668-11396687 Msa0315200:three_prime_UTR 45.0%
! CTTACCCTGCATTCTTTTCC+TGG + chr2_4:11394585-11394604 Msa0315200:five_prime_UTR 45.0%
AAAGAAGGATGGTGTTCCAG+AGG + chr2_4:11394941-11394960 Msa0315200:five_prime_UTR 45.0%
!! TTGATTCGCTAGGCTTTGGA+TGG + chr2_4:11395031-11395050 Msa0315200:five_prime_UTR 45.0%
TTTGGATGGCATGGAGAATG+AGG + chr2_4:11395045-11395064 Msa0315200:five_prime_UTR 45.0%
CAGAAGCTTTGCCATGAGTT+AGG + chr2_4:11395558-11395577 Msa0315200:intron 45.0%
GAGAAAGGCTTGAGGATCTA+AGG - chr2_4:11395713-11395732 None:intergenic 45.0%
GTTCACAATGGAAGAGGACA+CGG + chr2_4:11395850-11395869 Msa0315200:exon 45.0%
TACCTCTTCCACTGGTAACA+TGG - chr2_4:11395880-11395899 None:intergenic 45.0%
TCACATAGTACCTCTTCCAC+TGG - chr2_4:11395888-11395907 None:intergenic 45.0%
TACAGCAGCAAATCCCAATC+CGG - chr2_4:11396012-11396031 None:intergenic 45.0%
!! GTTGGATGGCTTTAATGGTC+TGG - chr2_4:11396070-11396089 None:intergenic 45.0%
!! GGTACGTTGGATGGCTTTAA+TGG - chr2_4:11396075-11396094 None:intergenic 45.0%
AGGGAAACAATCTGGTACGT+TGG - chr2_4:11396088-11396107 None:intergenic 45.0%
!! AATGGCATGAGAAGCACTCA+TGG + chr2_4:11396668-11396687 Msa0315200:three_prime_UTR 45.0%
!!! TTATGTTTTTTTTTTTATTT+TGG + chr2_4:11395213-11395232 Msa0315200:intron 5.0%
!!! TATGTTTTTTTTTTTATTTT+GGG + chr2_4:11395214-11395233 Msa0315200:intron 5.0%
!!! TTATGTTTTTTTTTTTATTT+TGG + chr2_4:11395213-11395232 Msa0315200:intron 5.0%
!!! TATGTTTTTTTTTTTATTTT+GGG + chr2_4:11395214-11395233 Msa0315200:intron 5.0%
GGGAACAAAACCAGAACCCA+AGG + chr2_4:11394667-11394686 Msa0315200:five_prime_UTR 50.0%
! CTTGCTACTGCTGCTTCCTT+GGG - chr2_4:11394686-11394705 None:intergenic 50.0%
! ACTTGCTACTGCTGCTTCCT+TGG - chr2_4:11394687-11394706 None:intergenic 50.0%
GGATGGTGTTCCAGAGGAAA+AGG + chr2_4:11394947-11394966 Msa0315200:five_prime_UTR 50.0%
! CTTGGGTCTACCTTTTCCTC+TGG - chr2_4:11394960-11394979 None:intergenic 50.0%
! GCGTTTGATTCGCTAGGCTT+TGG + chr2_4:11395027-11395046 Msa0315200:five_prime_UTR 50.0%
GAATGAGGCAAATTCGCGCA+CGG + chr2_4:11395060-11395079 Msa0315200:five_prime_UTR 50.0%
TGCCATGTTACCAGTGGAAG+AGG + chr2_4:11395875-11395894 Msa0315200:CDS 50.0%
GGGAACAAAACCAGAACCCA+AGG + chr2_4:11394667-11394686 Msa0315200:five_prime_UTR 50.0%
! CTTGCTACTGCTGCTTCCTT+GGG - chr2_4:11394686-11394705 None:intergenic 50.0%
! ACTTGCTACTGCTGCTTCCT+TGG - chr2_4:11394687-11394706 None:intergenic 50.0%
GGATGGTGTTCCAGAGGAAA+AGG + chr2_4:11394947-11394966 Msa0315200:five_prime_UTR 50.0%
! CTTGGGTCTACCTTTTCCTC+TGG - chr2_4:11394960-11394979 None:intergenic 50.0%
! GCGTTTGATTCGCTAGGCTT+TGG + chr2_4:11395027-11395046 Msa0315200:five_prime_UTR 50.0%
GAATGAGGCAAATTCGCGCA+CGG + chr2_4:11395060-11395079 Msa0315200:five_prime_UTR 50.0%
TGCCATGTTACCAGTGGAAG+AGG + chr2_4:11395875-11395894 Msa0315200:CDS 50.0%
! ACTGCTGCTTCCTTGGGTTC+TGG - chr2_4:11394680-11394699 None:intergenic 55.0%
!! TCGCTAGGCTTTGGATGGCA+TGG + chr2_4:11395036-11395055 Msa0315200:five_prime_UTR 55.0%
ACGGCTTGCCATGTTACCAG+TGG + chr2_4:11395869-11395888 Msa0315200:CDS 55.0%
AGCGACGTCGTTTGCACTCT+TGG - chr2_4:11396132-11396151 None:intergenic 55.0%
GACTGGTAGAGGGACGAAGA+AGG - chr2_4:11396308-11396327 None:intergenic 55.0%
! ACTGCTGCTTCCTTGGGTTC+TGG - chr2_4:11394680-11394699 None:intergenic 55.0%
!! TCGCTAGGCTTTGGATGGCA+TGG + chr2_4:11395036-11395055 Msa0315200:five_prime_UTR 55.0%
ACGGCTTGCCATGTTACCAG+TGG + chr2_4:11395869-11395888 Msa0315200:CDS 55.0%
AGCGACGTCGTTTGCACTCT+TGG - chr2_4:11396132-11396151 None:intergenic 55.0%
GACTGGTAGAGGGACGAAGA+AGG - chr2_4:11396308-11396327 None:intergenic 55.0%
!! GAGTTGATGTCGGCACGACG+AGG + chr2_4:11396200-11396219 Msa0315200:three_prime_UTR 60.0%
GTCGGCACGACGAGGTCATA+TGG + chr2_4:11396208-11396227 Msa0315200:three_prime_UTR 60.0%
! CGGCGCAACACTGATGGACA+AGG - chr2_4:11396246-11396265 None:intergenic 60.0%
CGACCTCTCCTTCTCGACGA+CGG - chr2_4:11396266-11396285 None:intergenic 60.0%
CGTCGAGAAGGAGAGGTCGT+CGG + chr2_4:11396267-11396286 Msa0315200:three_prime_UTR 60.0%
!! GAGTTGATGTCGGCACGACG+AGG + chr2_4:11396200-11396219 Msa0315200:three_prime_UTR 60.0%
GTCGGCACGACGAGGTCATA+TGG + chr2_4:11396208-11396227 Msa0315200:three_prime_UTR 60.0%
! CGGCGCAACACTGATGGACA+AGG - chr2_4:11396246-11396265 None:intergenic 60.0%
CGACCTCTCCTTCTCGACGA+CGG - chr2_4:11396266-11396285 None:intergenic 60.0%
CGTCGAGAAGGAGAGGTCGT+CGG + chr2_4:11396267-11396286 Msa0315200:three_prime_UTR 60.0%
CGACGACGGCGCAACACTGA+TGG - chr2_4:11396252-11396271 None:intergenic 65.0%
GTGTTGCGCCGTCGTCGAGA+AGG + chr2_4:11396255-11396274 Msa0315200:three_prime_UTR 65.0%
CGACGACGGCGCAACACTGA+TGG - chr2_4:11396252-11396271 None:intergenic 65.0%
GTGTTGCGCCGTCGTCGAGA+AGG + chr2_4:11396255-11396274 Msa0315200:three_prime_UTR 65.0%
CGCGCACGGAACGCTGCAAC+AGG + chr2_4:11395074-11395093 Msa0315200:five_prime_UTR 70.0%
GCGCCGTCGTCGAGAAGGAG+AGG + chr2_4:11396260-11396279 Msa0315200:three_prime_UTR 70.0%
CGCGCACGGAACGCTGCAAC+AGG + chr2_4:11395074-11395093 Msa0315200:five_prime_UTR 70.0%
GCGCCGTCGTCGAGAAGGAG+AGG + chr2_4:11396260-11396279 Msa0315200:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 11394520 11396733 11394520 ID=Msa0315200;Name=Msa0315200
chr2_4 mRNA 11394520 11396733 11394520 ID=Msa0315200-mRNA-1;Parent=Msa0315200;Name=Msa0315200-mRNA-1;_AED=0.17;_eAED=0.17;_QI=642|1|1|1|0.5|0.66|3|669|32
chr2_4 exon 11394520 11395095 11394520 ID=Msa0315200-mRNA-1:exon:4390;Parent=Msa0315200-mRNA-1
chr2_4 exon 11395791 11395896 11395791 ID=Msa0315200-mRNA-1:exon:4391;Parent=Msa0315200-mRNA-1
chr2_4 exon 11396006 11396733 11396006 ID=Msa0315200-mRNA-1:exon:4392;Parent=Msa0315200-mRNA-1
chr2_4 five_prime_UTR 11394520 11395095 11394520 ID=Msa0315200-mRNA-1:five_prime_utr;Parent=Msa0315200-mRNA-1
chr2_4 five_prime_UTR 11395791 11395856 11395791 ID=Msa0315200-mRNA-1:five_prime_utr;Parent=Msa0315200-mRNA-1
chr2_4 CDS 11395857 11395896 11395857 ID=Msa0315200-mRNA-1:cds;Parent=Msa0315200-mRNA-1
chr2_4 CDS 11396006 11396064 11396006 ID=Msa0315200-mRNA-1:cds;Parent=Msa0315200-mRNA-1
chr2_4 three_prime_UTR 11396065 11396733 11396065 ID=Msa0315200-mRNA-1:three_prime_utr;Parent=Msa0315200-mRNA-1
Gene Sequence

>Msa0315200

ATGGAAGAGGACACGGCTTGCCATGTTACCAGTGGAAGAGGTACCGGATTGGGATTTGCTGCTGTAAAAGGAATCCCGCAGTTACACACAGTTAAGTAA
Protein sequence

>Msa0315200

MEEDTACHVTSGRGTGLGFAAVKGIPQLHTVK*