AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1262420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1262420 MtrunA17_Chr4g0063841 93.819 631 39 0 17 647 3 633 0.0 1150
Msa1262420 MtrunA17_Chr1g0149351 61.228 619 230 4 35 647 29 643 0.0 737
Msa1262420 MtrunA17_Chr3g0137771 55.648 602 259 3 48 646 44 640 0.0 640
Msa1262420 MtrunA17_Chr4g0063861 82.564 390 68 0 258 647 1 390 0.0 622
Msa1262420 MtrunA17_Chr4g0063861 24.812 399 242 9 181 550 2 371 6.27e-19 89.4
Msa1262420 MtrunA17_Chr4g0063861 23.729 295 190 7 166 428 92 383 4.29e-11 65.5
Msa1262420 MtrunA17_Chr4g0063851 77.841 176 26 3 16 185 2 170 4.34e-85 266
Msa1262420 MtrunA17_Chr2g0286991 25.990 581 367 16 78 611 11 575 1.51e-39 154
Msa1262420 MtrunA17_Chr2g0286991 26.407 462 300 10 181 639 153 577 5.81e-35 140
Msa1262420 MtrunA17_Chr2g0286991 27.873 409 283 11 233 639 127 525 8.33e-25 109
Msa1262420 MtrunA17_Chr2g0286991 29.856 278 160 5 158 432 340 585 8.68e-24 106
Msa1262420 MtrunA17_Chr2g0286991 28.435 313 210 6 181 488 281 584 7.30e-22 100
Msa1262420 MtrunA17_Chr7g0272131 28.764 445 282 11 168 600 184 605 5.55e-30 125
Msa1262420 MtrunA17_Chr7g0272131 26.496 468 274 14 196 634 135 561 1.92e-21 99.0
Msa1262420 MtrunA17_Chr7g0272131 26.263 297 186 6 179 474 399 663 3.95e-19 91.7
Msa1262420 MtrunA17_Chr1g0212071 24.706 425 277 11 76 492 18 407 1.37e-25 109
Msa1262420 MtrunA17_Chr1g0212071 25.587 426 280 11 109 522 10 410 1.41e-17 85.5
Msa1262420 MtrunA17_Chr1g0212071 23.473 311 182 6 346 628 103 385 5.49e-16 80.9
Msa1262420 MtrunA17_Chr1g0212071 31.122 196 131 4 442 636 94 286 7.38e-16 80.1
Msa1262420 MtrunA17_Chr4g0004901 27.021 470 299 16 143 597 179 619 1.41e-25 112
Msa1262420 MtrunA17_Chr4g0004901 26.250 480 316 15 181 633 135 603 1.59e-24 108
Msa1262420 MtrunA17_Chr4g0004901 29.204 339 203 10 180 517 366 668 3.04e-21 98.6
Msa1262420 MtrunA17_Chr8g0347711 27.174 368 223 10 74 417 11 357 5.87e-20 92.4
Msa1262420 MtrunA17_Chr3g0125671 31.183 186 102 2 215 400 123 282 6.94e-18 85.9
Msa1262420 MtrunA17_Chr3g0125671 32.877 146 71 3 148 269 140 282 7.29e-11 64.3
Msa1262420 MtrunA17_Chr4g0008501 22.719 581 389 21 63 611 30 582 3.09e-17 85.5
Msa1262420 MtrunA17_Chr1g0150971 24.883 426 245 15 193 597 339 710 9.32e-16 81.3
Msa1262420 MtrunA17_Chr1g0176031 30.204 245 153 5 179 413 90 326 1.10e-15 79.0
Msa1262420 MtrunA17_Chr1g0176031 21.321 333 198 8 194 520 52 326 1.57e-14 75.5
Msa1262420 MtrunA17_Chr1g0176031 29.918 244 160 8 349 591 107 340 1.90e-14 75.5
Msa1262420 MtrunA17_Chr1g0152951 24.444 360 207 10 229 584 134 432 1.14e-13 73.9
Msa1262420 MtrunA17_Chr1g0152951 37.190 121 72 3 182 300 320 438 4.24e-11 65.9
Msa1262420 MtrunA17_Chr1g0159531 33.333 153 95 3 148 299 128 274 1.75e-13 72.4
Msa1262420 MtrunA17_Chr1g0159531 28.505 214 141 3 178 379 100 313 8.54e-13 70.1
Msa1262420 MtrunA17_Chr4g0070541 26.744 258 181 7 350 604 397 649 1.76e-13 73.9
Msa1262420 MtrunA17_Chr8g0352431 23.617 470 296 17 185 622 131 569 2.71e-12 69.7
Msa1262420 MtrunA17_Chr8g0342371 23.649 444 307 15 185 611 136 564 3.64e-12 69.3
Msa1262420 MtrunA17_Chr4g0018891 25.532 329 208 13 233 559 189 482 8.23e-12 68.2
Msa1262420 MtrunA17_Chr1g0150991 23.472 409 246 15 193 597 155 500 1.57e-11 67.4
Msa1262420 MtrunA17_Chr5g0436511 25.000 392 221 18 180 532 145 502 1.80e-11 67.4
Msa1262420 MtrunA17_Chr3g0079141 22.807 513 289 20 37 529 11 436 2.11e-11 66.6
Msa1262420 MtrunA17_Chr5g0446541 23.640 478 316 14 186 639 98 550 2.51e-11 66.6
Msa1262420 MtrunA17_Chr2g0300611 24.324 259 164 5 374 604 400 654 3.16e-11 66.6
Msa1262420 MtrunA17_Chr4g0070521 24.254 268 185 7 350 604 28 290 3.55e-11 65.1
Msa1262420 MtrunA17_Chr7g0251831 28.780 205 121 9 201 388 315 511 4.60e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1262420 AT2G25490.1 51.975 633 283 7 19 647 13 628 0.0 641
Msa1262420 AT5G25350.1 52.291 633 282 7 19 646 5 622 0.0 607
Msa1262420 AT4G15475.1 28.328 586 347 19 78 611 11 575 3.82e-43 164
Msa1262420 AT4G15475.1 27.378 347 214 6 143 488 275 584 1.23e-27 118
Msa1262420 AT4G15475.1 26.957 345 220 6 181 525 281 593 3.26e-23 104
Msa1262420 AT4G15475.1 24.118 340 223 9 323 633 109 442 3.37e-16 82.8
Msa1262420 AT4G15475.1 30.769 156 103 3 157 312 443 593 6.02e-12 69.3
Msa1262420 AT5G27920.1 25.615 488 302 11 165 600 90 568 1.14e-27 119
Msa1262420 AT5G23340.1 24.941 421 280 8 76 492 8 396 8.14e-24 104
Msa1262420 AT5G23340.1 26.804 291 203 6 310 594 85 371 3.12e-21 97.1
Msa1262420 AT5G23340.1 31.658 199 132 4 442 639 84 279 1.20e-18 89.4
Msa1262420 AT5G01720.1 26.200 500 298 17 181 624 85 569 2.29e-22 102
Msa1262420 AT5G01720.1 23.604 394 236 5 180 550 238 589 4.06e-20 95.5
Msa1262420 AT5G01720.1 29.412 170 89 2 206 350 443 606 5.02e-11 66.2
Msa1262420 AT5G01720.1 20.924 368 236 9 176 519 260 596 6.34e-11 65.9
Msa1262420 AT1G21410.2 28.351 194 113 2 207 400 104 271 6.85e-17 83.2
Msa1262420 AT1G21410.2 27.004 237 110 5 181 390 130 330 7.89e-14 73.9
Msa1262420 AT1G21410.1 28.351 194 113 2 207 400 104 271 6.85e-17 83.2
Msa1262420 AT1G21410.1 27.004 237 110 5 181 390 130 330 7.89e-14 73.9
Msa1262420 AT1G80630.1 22.222 414 274 10 195 573 108 508 9.31e-16 81.3
Msa1262420 AT3G58530.1 27.132 258 154 7 159 413 102 328 2.29e-15 78.6
Msa1262420 AT3G58530.1 21.557 334 197 7 194 520 53 328 1.50e-13 73.2
Msa1262420 AT3G58530.1 26.749 243 151 7 404 622 112 351 5.81e-12 68.2
Msa1262420 AT1G47056.1 26.239 343 190 12 206 520 116 423 3.29e-15 79.3
Msa1262420 AT1G47056.1 33.918 171 102 5 138 306 260 421 1.46e-13 73.9
Msa1262420 AT1G77000.2 28.947 228 143 5 181 390 104 330 3.02e-14 75.1
Msa1262420 AT1G77000.2 28.877 187 121 6 465 642 99 282 5.06e-11 65.5
Msa1262420 AT1G77000.1 28.947 228 143 5 181 390 104 330 3.02e-14 75.1
Msa1262420 AT1G77000.1 28.877 187 121 6 465 642 99 282 5.06e-11 65.5
Msa1262420 AT3G07550.1 23.098 368 239 8 66 422 6 340 2.74e-13 72.8
Msa1262420 AT3G07550.2 23.098 368 239 8 66 422 6 340 2.74e-13 72.8
Msa1262420 AT3G07550.3 24.167 360 218 8 66 422 6 313 6.49e-13 71.2
Msa1262420 AT4G30640.1 29.808 208 127 5 65 270 10 200 2.50e-12 68.6
Msa1262420 AT1G77000.3 28.324 173 98 2 207 379 104 250 1.45e-11 65.9
Msa1262420 AT1G77000.4 28.324 173 98 2 207 379 104 250 1.45e-11 65.9
Msa1262420 AT5G67250.1 23.913 368 196 10 230 584 117 413 5.51e-11 65.9
Msa1262420 AT4G07400.1 37.008 127 76 3 182 306 330 454 6.80e-11 65.9

Find 176 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAGCATGATTCAATGTTTA+AGG 0.094544 8_2:-65110985 None:intergenic
CGCTGGCCTTCTCCCTCTTT+TGG 0.142205 8_2:+65111730 Msa1262420:CDS
AGTTTCTCGTGGAGAATTTA+TGG 0.177171 8_2:+65112137 Msa1262420:CDS
CTGGCTGTTATTGGCCATTA+TGG 0.192129 8_2:+65111179 Msa1262420:CDS
CGCGATGGAACTGCTGAATA+AGG 0.264291 8_2:+65112182 Msa1262420:three_prime_UTR
ATGTTTAAGGTTGTCAAGTT+AGG 0.283107 8_2:-65110972 None:intergenic
TGTGTCTTGTTTGCTATATT+CGG 0.319855 8_2:+65111103 Msa1262420:CDS
TCGTTACTGCCTTTCCATAA+TGG 0.327153 8_2:-65111193 None:intergenic
GAATATTACTGATGCAAGTT+TGG 0.327338 8_2:+65111880 Msa1262420:CDS
TTTATGGAGATGTGATATTC+TGG 0.332414 8_2:+65112153 Msa1262420:CDS
CTGGAGAGATCTTGTGCATT+TGG 0.332649 8_2:-65111432 None:intergenic
CTCTCTATGTCTGACTGTTC+AGG 0.334066 8_2:+65112016 Msa1262420:CDS
AACTTGCATCAGTAATATTC+TGG 0.336340 8_2:-65111877 None:intergenic
TGTTATATTCTGTTTGTTGT+AGG 0.345967 8_2:+65112517 Msa1262420:three_prime_UTR
TGTCCTCTTGTTGGAGATCA+TGG 0.346783 8_2:+65111080 Msa1262420:CDS
ATTCAAAACTGTCCTGGTTT+CGG 0.358584 8_2:+65111626 Msa1262420:CDS
CTTGTCAAGGTGAACCTTGC+AGG 0.363717 8_2:+65111770 Msa1262420:CDS
AGGTGAACCTTGCAGGTTGC+TGG 0.365852 8_2:+65111777 Msa1262420:CDS
GCTGAATAAGGAAATGCTAA+TGG 0.368749 8_2:+65112194 Msa1262420:three_prime_UTR
GTGCCTTTCCTGGTGAAATT+AGG 0.368798 8_2:+65112055 Msa1262420:CDS
ACTCTAGATCGCACATTGCC+TGG 0.374175 8_2:+65112472 Msa1262420:three_prime_UTR
TGCGAAGGCACATCTGCTTC+AGG 0.374917 8_2:-65111370 None:intergenic
ATTTACACTTGTGAAGTGTA+TGG 0.378579 8_2:+65111538 Msa1262420:CDS
ATCAGTGCCTCATTTACCTT+TGG 0.379132 8_2:+65110390 Msa1262420:CDS
GGCTTGCTGCTATTGCTGTT+GGG 0.383869 8_2:+65110694 Msa1262420:CDS
GAAATTAGGCCAGGCGTTGT+CGG 0.389515 8_2:+65112069 Msa1262420:CDS
GCTGCTATTGCTGTTGGGAC+TGG 0.389778 8_2:+65110699 Msa1262420:CDS
CTGTGGAATGTGTCTTCTAT+TGG 0.391657 8_2:+65110837 Msa1262420:CDS
TATTGCAATCAAACCCTTGT+TGG 0.403057 8_2:-65110937 None:intergenic
TGTGTCTTCTATTGGTGATA+AGG 0.403727 8_2:+65110845 Msa1262420:CDS
ACTGGTTGCCGTGGTGGACT+TGG 0.405676 8_2:+65110717 Msa1262420:CDS
GTGTCTTCTATTGGTGATAA+GGG 0.408571 8_2:+65110846 Msa1262420:CDS
GAACAAGTGTGTGCCTTTCC+TGG 0.410656 8_2:+65112045 Msa1262420:CDS
ATTGAATCATGCTCGATGAT+TGG 0.411828 8_2:+65110993 Msa1262420:CDS
CAGATCAACATGCTGAAGTT+GGG 0.413419 8_2:-65111684 None:intergenic
TCCCTTCCAAACGCCTTGTA+AGG 0.414436 8_2:-65110653 None:intergenic
TCAGCAGTTCCATCGCGATT+AGG 0.414562 8_2:-65112176 None:intergenic
AGGCTTGCTGCTATTGCTGT+TGG 0.416928 8_2:+65110693 Msa1262420:CDS
CTTGGAAAGCTATCTATTAG+AGG 0.419353 8_2:+65110735 Msa1262420:CDS
TGGCTACCTTACAAGGCGTT+TGG 0.422778 8_2:+65110647 Msa1262420:CDS
TCATGGAACTATAGCCAAAA+GGG 0.423795 8_2:-65112415 None:intergenic
GAGCTTCTCTCTTTGCCACT+TGG 0.430900 8_2:-65110507 None:intergenic
CTCATGGAACTATAGCCAAA+AGG 0.433792 8_2:-65112416 None:intergenic
TCCATGTTGGCCAGGCTACA+TGG 0.434359 8_2:+65111821 Msa1262420:CDS
TGTTTAAGGTTGTCAAGTTA+GGG 0.434609 8_2:-65110971 None:intergenic
ATCAAGGATTGTCCTCTTGT+TGG 0.435290 8_2:+65111071 Msa1262420:CDS
GGTAATGATGAATTGCATCC+TGG 0.437251 8_2:+65110303 Msa1262420:intron
TGCGGCGCGGTTTGTTGACT+CGG 0.438068 8_2:+65112307 Msa1262420:three_prime_UTR
GTTGATCTGACTGGACTTTG+CGG 0.440114 8_2:+65111698 Msa1262420:CDS
GACTATCAAGTGTGGATTTG+CGG 0.442563 8_2:+65112289 Msa1262420:three_prime_UTR
TGTTGGTGATCGACGAAGAA+TGG 0.444320 8_2:-65110920 None:intergenic
AAATTAGGCCAGGCGTTGTC+GGG 0.445281 8_2:+65112070 Msa1262420:CDS
TGGAAAGGCAGTAACGAATC+TGG 0.449057 8_2:+65111199 Msa1262420:CDS
GCCTACCATTGAGATCCTTC+CGG 0.449888 8_2:+65110452 Msa1262420:CDS
TTGAATAACCTTGCCTTACA+AGG 0.450924 8_2:-65109176 None:intergenic
AGTTACTGAGTCTTGGTTGA+TGG 0.456016 8_2:+65112444 Msa1262420:three_prime_UTR
GTTACATTCAATCTGTATCA+AGG 0.456342 8_2:+65111055 Msa1262420:CDS
AAGCTTTACCTTTGACAAGT+TGG 0.456600 8_2:-65111129 None:intergenic
TATTCAAGCCCGACAACGCC+TGG 0.460376 8_2:-65112078 None:intergenic
AGTGGCAAAGAGAGAAGCTC+AGG 0.464804 8_2:+65110510 Msa1262420:CDS
CTCTAGATCGCACATTGCCT+GGG 0.467454 8_2:+65112473 Msa1262420:three_prime_UTR
AGACATCTAGATCATTCAAC+AGG 0.469504 8_2:-65111925 None:intergenic
TCAGATCAACATGCTGAAGT+TGG 0.473467 8_2:-65111685 None:intergenic
GGTCTTTGTGAGATTGCAAA+AGG 0.475591 8_2:+65110867 Msa1262420:CDS
TTAACCATTCAAAACTGTCC+TGG 0.477543 8_2:+65111620 Msa1262420:CDS
GGCATAGAAAATCCCACATT+GGG 0.478588 8_2:-65111486 None:intergenic
ATATGGTTAACCTCCTTGTA+AGG 0.479102 8_2:+65109163 None:intergenic
ACTCTTCCAACTGCAAGCTC+TGG 0.480930 8_2:-65111451 None:intergenic
GATACAGATTGAATGTAACT+TGG 0.482768 8_2:-65111051 None:intergenic
TTCAGCGAGCTCTTGAGCAC+GGG 0.483184 8_2:+65110418 Msa1262420:CDS
CAGGTGCCTGTGTATCAAAA+AGG 0.484862 8_2:+65110529 Msa1262420:CDS
GCCTCACAATTCTCCAAAAG+AGG 0.487248 8_2:-65111743 None:intergenic
TTTCCTGGTGAAATTAGGCC+AGG 0.489686 8_2:+65112060 Msa1262420:CDS
GAGAGTTCCACCATGTAGCC+TGG 0.492311 8_2:-65111831 None:intergenic
CTTTGTATCCGACATTGGAC+TGG 0.492327 8_2:+65111400 Msa1262420:CDS
ACATAGTTTCCATGTTGGCC+AGG 0.504234 8_2:+65111813 Msa1262420:CDS
GGCACATCTGCTTCAGGTGT+GGG 0.504975 8_2:-65111364 None:intergenic
CTTCAGCGAGCTCTTGAGCA+CGG 0.505562 8_2:+65110417 Msa1262420:CDS
TGGTTTCGGCAGTGCTAGCA+TGG 0.505703 8_2:+65111640 Msa1262420:CDS
AGTGCTAGCATGGCTGTGGT+TGG 0.506787 8_2:+65111650 Msa1262420:CDS
ATGCTCGATGATTGGAAATG+AGG 0.506806 8_2:+65111001 Msa1262420:CDS
TGCTCGCATCGGTTACCCCT+TGG 0.507539 8_2:-65111319 None:intergenic
TGCTCGATGATTGGAAATGA+GGG 0.508764 8_2:+65111002 Msa1262420:CDS
CGCATCGGTTACCCCTTGGC+AGG 0.510702 8_2:-65111315 None:intergenic
AAGTGTGCAATCACTGATGC+AGG 0.512250 8_2:+65111950 Msa1262420:CDS
GGTCTCTCAGCAGTTGCTCA+TGG 0.513210 8_2:+65110789 Msa1262420:CDS
GGAGGAAAGCCACTGATGTA+AGG 0.514553 8_2:+65110673 Msa1262420:CDS
ATCTCTCCAGAGCTTGCAGT+TGG 0.514820 8_2:+65111445 Msa1262420:CDS
TGCGTCATGGGAGTTGCTCA+AGG 0.518086 8_2:+65111260 Msa1262420:CDS
ACCATGTAGCCTGGCCAACA+TGG 0.519235 8_2:-65111822 None:intergenic
AAGGTGATGGCTACCTTACA+AGG 0.519297 8_2:+65110640 Msa1262420:CDS
GGAAACAAACCAAAACTCCT+TGG 0.519774 8_2:-65112337 None:intergenic
GTGCTAGCATGGCTGTGGTT+GGG 0.523567 8_2:+65111651 Msa1262420:CDS
TACCTTACAAGGCGTTTGGA+AGG 0.525492 8_2:+65110651 Msa1262420:CDS
AGCATCGAAGCCATGGGTAA+AGG 0.526861 8_2:+65111338 Msa1262420:CDS
TTTACACTTGTGAAGTGTAT+GGG 0.528626 8_2:+65111539 Msa1262420:CDS
TGTAGCTTTGTATCCGACAT+TGG 0.531778 8_2:+65111395 Msa1262420:CDS
CTTCAGCATGTTGATCTGAC+TGG 0.536194 8_2:+65111689 Msa1262420:CDS
ATGAAATTGGGCCGTTGAGG+AGG 0.536524 8_2:-65109202 None:intergenic
TGCAAAAGGATGTCATATGT+TGG 0.540414 8_2:+65110881 Msa1262420:CDS
CTGATTCTAGTTCGCTTCGT+AGG 0.543276 8_2:-65110372 None:intergenic
AGATTACATAGTTTCCATGT+TGG 0.543872 8_2:+65111808 Msa1262420:CDS
GTTGGGACTGGTTGCCGTGG+TGG 0.544249 8_2:+65110711 Msa1262420:CDS
GTAATGATGAATTGCATCCT+GGG 0.545999 8_2:+65110304 Msa1262420:CDS
ATCTCAAAGAGACATTCGTC+CGG 0.551142 8_2:-65110471 None:intergenic
ACTCAGTAACTGCATTCTCA+TGG 0.552061 8_2:-65112432 None:intergenic
TTCGGCTTCCAACTTGTCAA+AGG 0.552626 8_2:+65111121 Msa1262420:CDS
ATGCTAGCACTGCCGAAACC+AGG 0.554879 8_2:-65111638 None:intergenic
CGGCAGTGCTAGCATGGCTG+TGG 0.556009 8_2:+65111646 Msa1262420:CDS
ATTAATAATGAAGCAGCAGA+TGG 0.557908 8_2:+65112234 Msa1262420:three_prime_UTR
ATACAGATTGAATGTAACTT+GGG 0.559199 8_2:-65111050 None:intergenic
AGGCACATCTGCTTCAGGTG+TGG 0.560606 8_2:-65111365 None:intergenic
AGAATGCAGTTACTGAGTCT+TGG 0.560746 8_2:+65112437 Msa1262420:three_prime_UTR
GTGAAAGTGATCTGAGAGAA+GGG 0.561763 8_2:-65110815 None:intergenic
TGGCCTAATTTCACCAGGAA+AGG 0.564441 8_2:-65112058 None:intergenic
ATGATCAGCACGAGAGAGAA+CGG 0.564713 8_2:-65111978 None:intergenic
ACCTTACAAGGCGTTTGGAA+GGG 0.565254 8_2:+65110652 Msa1262420:CDS
AGCTCGCTGAAGAGCTCCAA+AGG 0.565614 8_2:-65110406 None:intergenic
GTTAACCTCCTTGTAAGGCA+AGG 0.573809 8_2:+65109168 Msa1262420:CDS
TAACTATTACTTCCTGCCAA+GGG 0.575789 8_2:+65111303 Msa1262420:CDS
TTTATGAAATTGGGCCGTTG+AGG 0.576561 8_2:-65109205 None:intergenic
AAGAGCTCCAAAGGTAAATG+AGG 0.576760 8_2:-65110397 None:intergenic
GGTTTGATTGCAATAGCTGA+AGG 0.577016 8_2:+65110945 Msa1262420:CDS
CTCGAAATACTAAACCTCGA+TGG 0.579655 8_2:+65111851 Msa1262420:CDS
CAAAGAGACATTCGTCCGGA+AGG 0.580269 8_2:-65110467 None:intergenic
GTGTGGGAAACCTTTACCCA+TGG 0.581206 8_2:-65111348 None:intergenic
AGCAGCAAGCCTTACATCAG+TGG 0.584954 8_2:-65110682 None:intergenic
GGGCATAGAAAATCCCACAT+TGG 0.585502 8_2:-65111487 None:intergenic
CAATGCAATTGAGTTTCTCG+TGG 0.586036 8_2:+65112126 Msa1262420:CDS
TTCTTCGTCGATCACCAACA+AGG 0.587327 8_2:+65110923 Msa1262420:CDS
AGAGCAGTGCACATCAACAT+TGG 0.587717 8_2:-65110349 None:intergenic
GTCTCTTTGAGATATTCAGA+CGG 0.588078 8_2:+65110481 Msa1262420:CDS
GGGAATTAAAGACATTGATG+TGG 0.591377 8_2:+65111559 Msa1262420:CDS
TTACAAGGCGTTTGGAAGGG+AGG 0.593323 8_2:+65110655 Msa1262420:CDS
CCTCACAATTCTCCAAAAGA+GGG 0.598079 8_2:-65111742 None:intergenic
ATATTCTGGCCTAATCGCGA+TGG 0.598949 8_2:+65112167 Msa1262420:exon
TGTTATTGGCCATTATGGAA+AGG 0.600886 8_2:+65111184 Msa1262420:CDS
CTCTCAGATCACTTTCACTG+TGG 0.601821 8_2:+65110820 Msa1262420:CDS
AGTGAAAGTGATCTGAGAGA+AGG 0.602549 8_2:-65110816 None:intergenic
CATGGCTTCGATGCTCGCAT+CGG 0.603091 8_2:-65111330 None:intergenic
GATGAAGATGCTGAAGGTGA+TGG 0.605262 8_2:+65110627 Msa1262420:CDS
AGGCAATGTGCGATCTAGAG+TGG 0.606144 8_2:-65112470 None:intergenic
TGTAACAGATTCACCCAATG+TGG 0.606794 8_2:+65111473 Msa1262420:CDS
AGATCAACATGCTGAAGTTG+GGG 0.609536 8_2:-65111683 None:intergenic
CTAACTATTACTTCCTGCCA+AGG 0.610927 8_2:+65111302 Msa1262420:CDS
ACACCATGATCTCCAACAAG+AGG 0.615650 8_2:-65111083 None:intergenic
TCTTCGTCGATCACCAACAA+GGG 0.621934 8_2:+65110924 Msa1262420:CDS
AAATGCTGCCAGTCCAATGT+CGG 0.622406 8_2:-65111408 None:intergenic
AATTCTCCAAAAGAGGGAGA+AGG 0.622832 8_2:-65111736 None:intergenic
GATGCGAGCATCGAAGCCAT+GGG 0.623164 8_2:+65111332 Msa1262420:CDS
TTCGTCCGGAAGGATCTCAA+TGG 0.627959 8_2:-65110457 None:intergenic
ATACAAAGCTACATCTGCGA+AGG 0.636036 8_2:-65111385 None:intergenic
TCAAGTGTGGATTTGCGGCG+CGG 0.636183 8_2:+65112294 Msa1262420:three_prime_UTR
TTATTCAACAACCTCCTCAA+CGG 0.640721 8_2:+65109191 Msa1262420:CDS
GTAACGAATCTGGTTCTAAG+TGG 0.640999 8_2:+65111209 Msa1262420:CDS
TAATGATGAATTGCATCCTG+GGG 0.643262 8_2:+65110305 Msa1262420:CDS
GCTGTTGGGACTGGTTGCCG+TGG 0.645340 8_2:+65110708 Msa1262420:CDS
TCAAGTTCCAGCAACCTGCA+AGG 0.650087 8_2:-65111784 None:intergenic
TTGTGAGGCTGGACTTGTCA+AGG 0.655559 8_2:+65111757 Msa1262420:CDS
GCTGATATGACTATCAAGTG+TGG 0.658205 8_2:+65112281 Msa1262420:three_prime_UTR
AACTATTACTTCCTGCCAAG+GGG 0.659376 8_2:+65111304 Msa1262420:CDS
GAGCAACTGCTGAGAGACCG+CGG 0.662248 8_2:-65110785 None:intergenic
TAATATTCTGGCATCCATCG+AGG 0.665697 8_2:-65111865 None:intergenic
CATGGAACTATAGCCAAAAG+GGG 0.666185 8_2:-65112414 None:intergenic
GTAACAGATTCACCCAATGT+GGG 0.667862 8_2:+65111474 Msa1262420:CDS
GAGAGTGATGAAGATGCTGA+AGG 0.668726 8_2:+65110621 Msa1262420:CDS
AATAACCTTGCCTTACAAGG+AGG 0.669394 8_2:-65109173 None:intergenic
TCCGGAAGGATCTCAATGGT+AGG 0.673037 8_2:-65110453 None:intergenic
AGAGGAAACAACTCGACTCG+AGG 0.676030 8_2:+65110753 Msa1262420:CDS
AAAGATCGAAGAGACTTGCA+AGG 0.680643 8_2:-65111599 None:intergenic
AGTGATACAAAGTAAAACCC+AGG 0.687051 8_2:-65112490 None:intergenic
ATATTCAGACGGCTTCCAAG+TGG 0.698602 8_2:+65110492 Msa1262420:CDS
ATGTTGGCCAGGCTACATGG+TGG 0.717767 8_2:+65111824 Msa1262420:CDS
ACTCGAGGTGTTACAGACCG+CGG 0.719268 8_2:+65110768 Msa1262420:CDS
AATGATGAATTGCATCCTGG+GGG 0.735856 8_2:+65110306 Msa1262420:CDS
ATAGCTTTCCAAGTCCACCA+CGG 0.754154 8_2:-65110725 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTTATATCATTATATTATA+TGG + chr8_2:65109941-65109960 Msa1262420:intron 10.0%
!!! GAAGTTAATTTTTTTTTTTT+TGG + chr8_2:65110066-65110085 Msa1262420:intron 10.0%
!!! TTTACCTTTTTTATGAAATT+GGG - chr8_2:65109217-65109236 None:intergenic 15.0%
!!! TTTTACCTTTTTTATGAAAT+TGG - chr8_2:65109218-65109237 None:intergenic 15.0%
!! ATAAAAAGAAATATAAAGAG+TGG + chr8_2:65109283-65109302 Msa1262420:intron 15.0%
!!! CATTAAAAAAATCGATTTTA+AGG + chr8_2:65109376-65109395 Msa1262420:intron 15.0%
!!! TTTTTAAGTTTTCTAGTTTA+TGG + chr8_2:65109861-65109880 Msa1262420:intron 15.0%
!! AAGAATAACAAAGAAAATAT+TGG + chr8_2:65110009-65110028 Msa1262420:intron 15.0%
!!! TTTATGTCTTCAATTGTTTT+GGG - chr8_2:65109268-65109287 None:intergenic 20.0%
!!! TTTTATGTCTTCAATTGTTT+TGG - chr8_2:65109269-65109288 None:intergenic 20.0%
!! GGAAATAGAAAAAAAATCTT+AGG - chr8_2:65109463-65109482 None:intergenic 20.0%
!! ACTAGAAAACTTAAAAATTG+AGG - chr8_2:65109858-65109877 None:intergenic 20.0%
!!! TTTCTTTGTTATTCTTACTA+AGG - chr8_2:65110005-65110024 None:intergenic 20.0%
!! TTGTAATGATAAGTTGTAAT+TGG + chr8_2:65110227-65110246 Msa1262420:intron 20.0%
!! TTAAAAATGCTTGCAAAAAA+GGG - chr8_2:65112370-65112389 None:intergenic 20.0%
!! ATTAAAAATGCTTGCAAAAA+AGG - chr8_2:65112371-65112390 None:intergenic 20.0%
!! AGAAATATAAAGAGTGGTTT+TGG + chr8_2:65109289-65109308 Msa1262420:intron 25.0%
!!! TTATGGTTTTAATTGATCCT+AGG + chr8_2:65109878-65109897 Msa1262420:intron 25.0%
! TTATGTTATGCTATAATCCT+AGG - chr8_2:65109898-65109917 None:intergenic 25.0%
! TATGGATGTTATATATCTTG+TGG + chr8_2:65109959-65109978 Msa1262420:intron 25.0%
!!! AGAACCCTTTTTATTTTCTT+TGG + chr8_2:65110098-65110117 Msa1262420:intron 25.0%
! TCCACCAAAGAAAATAAAAA+GGG - chr8_2:65110105-65110124 None:intergenic 25.0%
! TTCCACCAAAGAAAATAAAA+AGG - chr8_2:65110106-65110125 None:intergenic 25.0%
!!! TTTTATTTTCTTTGGTGGAA+TGG + chr8_2:65110106-65110125 Msa1262420:intron 25.0%
!!! AGATAGTGAATTTTGTTTCT+AGG + chr8_2:65110196-65110215 Msa1262420:intron 25.0%
!!! GATAGTGAATTTTGTTTCTA+GGG + chr8_2:65110197-65110216 Msa1262420:intron 25.0%
! ATACAGATTGAATGTAACTT+GGG - chr8_2:65111053-65111072 None:intergenic 25.0%
! TTAAAAATGTAAGCGAAAGA+GGG + chr8_2:65111234-65111253 Msa1262420:CDS 25.0%
!!! TGTTATATTCTGTTTGTTGT+AGG + chr8_2:65112517-65112536 Msa1262420:three_prime_UTR 25.0%
!!! GTTTTGGTTTTTTGTGAAAG+TGG + chr8_2:65109305-65109324 Msa1262420:intron 30.0%
GTAACATTGTACTTATTCTG+TGG + chr8_2:65109430-65109449 Msa1262420:intron 30.0%
! ACAAAGCGGTTTTTATATGT+TGG - chr8_2:65109484-65109503 None:intergenic 30.0%
TTGTCATGTAACAAACAAAG+CGG - chr8_2:65109498-65109517 None:intergenic 30.0%
GGAGCAAAAAAATGCAAAAA+AGG - chr8_2:65109585-65109604 None:intergenic 30.0%
!!! TTGCATTTTTTTGCTCCTTA+AGG + chr8_2:65109588-65109607 Msa1262420:intron 30.0%
GAGGTATATAACAGAGAAAA+GGG - chr8_2:65109640-65109659 None:intergenic 30.0%
! AGAGGTATATAACAGAGAAA+AGG - chr8_2:65109641-65109660 None:intergenic 30.0%
GAATAATGAACAAGAAAGAG+AGG - chr8_2:65109659-65109678 None:intergenic 30.0%
TGTGATATTCAGCTTATACT+TGG + chr8_2:65109693-65109712 Msa1262420:intron 30.0%
!!! ACCCTTTTTATTTTCTTTGG+TGG + chr8_2:65110101-65110120 Msa1262420:intron 30.0%
!! ATTGTGTGAATGACTTTTTC+AGG + chr8_2:65110282-65110301 Msa1262420:intron 30.0%
! TGTTTAAGGTTGTCAAGTTA+GGG - chr8_2:65110974-65110993 None:intergenic 30.0%
! ATGTTTAAGGTTGTCAAGTT+AGG - chr8_2:65110975-65110994 None:intergenic 30.0%
GATACAGATTGAATGTAACT+TGG - chr8_2:65111054-65111073 None:intergenic 30.0%
GTTACATTCAATCTGTATCA+AGG + chr8_2:65111055-65111074 Msa1262420:CDS 30.0%
!! TGTGTCTTGTTTGCTATATT+CGG + chr8_2:65111103-65111122 Msa1262420:CDS 30.0%
!!! GAATATCACTGATTTTTCTC+TGG + chr8_2:65111160-65111179 Msa1262420:CDS 30.0%
CTTAAAAATGTAAGCGAAAG+AGG + chr8_2:65111233-65111252 Msa1262420:CDS 30.0%
!! CTTCGTTTTGATGTTTGAAA+GGG - chr8_2:65111510-65111529 None:intergenic 30.0%
!! ACTTCGTTTTGATGTTTGAA+AGG - chr8_2:65111511-65111530 None:intergenic 30.0%
ATTTACACTTGTGAAGTGTA+TGG + chr8_2:65111538-65111557 Msa1262420:CDS 30.0%
TTTACACTTGTGAAGTGTAT+GGG + chr8_2:65111539-65111558 Msa1262420:CDS 30.0%
AGATTACATAGTTTCCATGT+TGG + chr8_2:65111808-65111827 Msa1262420:CDS 30.0%
AACTTGCATCAGTAATATTC+TGG - chr8_2:65111880-65111899 None:intergenic 30.0%
GAATATTACTGATGCAAGTT+TGG + chr8_2:65111880-65111899 Msa1262420:CDS 30.0%
TTTATGGAGATGTGATATTC+TGG + chr8_2:65112153-65112172 Msa1262420:CDS 30.0%
ATTAATAATGAAGCAGCAGA+TGG + chr8_2:65112234-65112253 Msa1262420:three_prime_UTR 30.0%
!! ACATTCAGCATACTTGTTTT+TGG + chr8_2:65112259-65112278 Msa1262420:three_prime_UTR 30.0%
!!! TTGTTTTTTACAACCCCTTT+TGG + chr8_2:65112401-65112420 Msa1262420:three_prime_UTR 30.0%
TTGAATAACCTTGCCTTACA+AGG - chr8_2:65109179-65109198 None:intergenic 35.0%
TTATTCAACAACCTCCTCAA+CGG + chr8_2:65109191-65109210 Msa1262420:CDS 35.0%
ACGGCCCAATTTCATAAAAA+AGG + chr8_2:65109210-65109229 Msa1262420:CDS 35.0%
!!! TGCTCCTTAAGGAGTTTTTT+TGG + chr8_2:65109599-65109618 Msa1262420:intron 35.0%
GATGCCAAAAAAACTCCTTA+AGG - chr8_2:65109606-65109625 None:intergenic 35.0%
GTAATGATGAATTGCATCCT+GGG + chr8_2:65110304-65110323 Msa1262420:CDS 35.0%
TAATGATGAATTGCATCCTG+GGG + chr8_2:65110305-65110324 Msa1262420:CDS 35.0%
!! GTCTCTTTGAGATATTCAGA+CGG + chr8_2:65110481-65110500 Msa1262420:CDS 35.0%
CTTGGAAAGCTATCTATTAG+AGG + chr8_2:65110735-65110754 Msa1262420:CDS 35.0%
! TGTGTCTTCTATTGGTGATA+AGG + chr8_2:65110845-65110864 Msa1262420:CDS 35.0%
! GTGTCTTCTATTGGTGATAA+GGG + chr8_2:65110846-65110865 Msa1262420:CDS 35.0%
TGCAAAAGGATGTCATATGT+TGG + chr8_2:65110881-65110900 Msa1262420:CDS 35.0%
TATTGCAATCAAACCCTTGT+TGG - chr8_2:65110940-65110959 None:intergenic 35.0%
CGAGCATGATTCAATGTTTA+AGG - chr8_2:65110988-65111007 None:intergenic 35.0%
ATTGAATCATGCTCGATGAT+TGG + chr8_2:65110993-65111012 Msa1262420:CDS 35.0%
AAGCTTTACCTTTGACAAGT+TGG - chr8_2:65111132-65111151 None:intergenic 35.0%
! GATTTTTCTCTGGCTGTTAT+TGG + chr8_2:65111170-65111189 Msa1262420:CDS 35.0%
TGTTATTGGCCATTATGGAA+AGG + chr8_2:65111184-65111203 Msa1262420:CDS 35.0%
TAACTATTACTTCCTGCCAA+GGG + chr8_2:65111303-65111322 Msa1262420:CDS 35.0%
GGGAATTAAAGACATTGATG+TGG + chr8_2:65111559-65111578 Msa1262420:CDS 35.0%
TTAACCATTCAAAACTGTCC+TGG + chr8_2:65111620-65111639 Msa1262420:CDS 35.0%
ATTCAAAACTGTCCTGGTTT+CGG + chr8_2:65111626-65111645 Msa1262420:CDS 35.0%
AGACATCTAGATCATTCAAC+AGG - chr8_2:65111928-65111947 None:intergenic 35.0%
AGTTTCTCGTGGAGAATTTA+TGG + chr8_2:65112137-65112156 Msa1262420:CDS 35.0%
GCTGAATAAGGAAATGCTAA+TGG + chr8_2:65112194-65112213 Msa1262420:three_prime_UTR 35.0%
TCATGGAACTATAGCCAAAA+GGG - chr8_2:65112418-65112437 None:intergenic 35.0%
AGTGATACAAAGTAAAACCC+AGG - chr8_2:65112493-65112512 None:intergenic 35.0%
! TGGGTTTTACTTTGTATCAC+TGG + chr8_2:65112492-65112511 Msa1262420:three_prime_UTR 35.0%
AATAACCTTGCCTTACAAGG+AGG - chr8_2:65109176-65109195 None:intergenic 40.0%
TTTATGAAATTGGGCCGTTG+AGG - chr8_2:65109208-65109227 None:intergenic 40.0%
! TGTGAAAGTGGTATTTGCTG+AGG + chr8_2:65109317-65109336 Msa1262420:intron 40.0%
AGTTACCACTCTGATTGACA+CGG - chr8_2:65109754-65109773 None:intergenic 40.0%
TGTGGCATGTTGTTGCAATA+AGG + chr8_2:65109977-65109996 Msa1262420:intron 40.0%
GTGGCATGTTGTTGCAATAA+GGG + chr8_2:65109978-65109997 Msa1262420:intron 40.0%
GGTAATGATGAATTGCATCC+TGG + chr8_2:65110303-65110322 Msa1262420:intron 40.0%
AATGATGAATTGCATCCTGG+GGG + chr8_2:65110306-65110325 Msa1262420:CDS 40.0%
ATCAGTGCCTCATTTACCTT+TGG + chr8_2:65110390-65110409 Msa1262420:CDS 40.0%
AAGAGCTCCAAAGGTAAATG+AGG - chr8_2:65110400-65110419 None:intergenic 40.0%
ATCTCAAAGAGACATTCGTC+CGG - chr8_2:65110474-65110493 None:intergenic 40.0%
! AAGCCACCTTTTTGATACAC+AGG - chr8_2:65110538-65110557 None:intergenic 40.0%
GTGAAAGTGATCTGAGAGAA+GGG - chr8_2:65110818-65110837 None:intergenic 40.0%
AGTGAAAGTGATCTGAGAGA+AGG - chr8_2:65110819-65110838 None:intergenic 40.0%
CTGTGGAATGTGTCTTCTAT+TGG + chr8_2:65110837-65110856 Msa1262420:CDS 40.0%
GGTCTTTGTGAGATTGCAAA+AGG + chr8_2:65110867-65110886 Msa1262420:CDS 40.0%
GGTTTGATTGCAATAGCTGA+AGG + chr8_2:65110945-65110964 Msa1262420:CDS 40.0%
ATGCTCGATGATTGGAAATG+AGG + chr8_2:65111001-65111020 Msa1262420:CDS 40.0%
TGCTCGATGATTGGAAATGA+GGG + chr8_2:65111002-65111021 Msa1262420:CDS 40.0%
ATCAAGGATTGTCCTCTTGT+TGG + chr8_2:65111071-65111090 Msa1262420:CDS 40.0%
TCGTTACTGCCTTTCCATAA+TGG - chr8_2:65111196-65111215 None:intergenic 40.0%
!! GTAACGAATCTGGTTCTAAG+TGG + chr8_2:65111209-65111228 Msa1262420:CDS 40.0%
CTAACTATTACTTCCTGCCA+AGG + chr8_2:65111302-65111321 Msa1262420:CDS 40.0%
AACTATTACTTCCTGCCAAG+GGG + chr8_2:65111304-65111323 Msa1262420:CDS 40.0%
ATACAAAGCTACATCTGCGA+AGG - chr8_2:65111388-65111407 None:intergenic 40.0%
!! TGTAGCTTTGTATCCGACAT+TGG + chr8_2:65111395-65111414 Msa1262420:CDS 40.0%
TGTAACAGATTCACCCAATG+TGG + chr8_2:65111473-65111492 Msa1262420:CDS 40.0%
GTAACAGATTCACCCAATGT+GGG + chr8_2:65111474-65111493 Msa1262420:CDS 40.0%
GGCATAGAAAATCCCACATT+GGG - chr8_2:65111489-65111508 None:intergenic 40.0%
AAAGATCGAAGAGACTTGCA+AGG - chr8_2:65111602-65111621 None:intergenic 40.0%
!!! GAAACCAGGACAGTTTTGAA+TGG - chr8_2:65111627-65111646 None:intergenic 40.0%
AGATCAACATGCTGAAGTTG+GGG - chr8_2:65111686-65111705 None:intergenic 40.0%
CAGATCAACATGCTGAAGTT+GGG - chr8_2:65111687-65111706 None:intergenic 40.0%
TCAGATCAACATGCTGAAGT+TGG - chr8_2:65111688-65111707 None:intergenic 40.0%
AATTCTCCAAAAGAGGGAGA+AGG - chr8_2:65111739-65111758 None:intergenic 40.0%
CCTCACAATTCTCCAAAAGA+GGG - chr8_2:65111745-65111764 None:intergenic 40.0%
CTCGAAATACTAAACCTCGA+TGG + chr8_2:65111851-65111870 Msa1262420:CDS 40.0%
TAATATTCTGGCATCCATCG+AGG - chr8_2:65111868-65111887 None:intergenic 40.0%
CAATGCAATTGAGTTTCTCG+TGG + chr8_2:65112126-65112145 Msa1262420:CDS 40.0%
GCTGATATGACTATCAAGTG+TGG + chr8_2:65112281-65112300 Msa1262420:three_prime_UTR 40.0%
GACTATCAAGTGTGGATTTG+CGG + chr8_2:65112289-65112308 Msa1262420:three_prime_UTR 40.0%
!! GGTTGTTTTCCAAGGAGTTT+TGG + chr8_2:65112328-65112347 Msa1262420:three_prime_UTR 40.0%
GGAAACAAACCAAAACTCCT+TGG - chr8_2:65112340-65112359 None:intergenic 40.0%
! CTTGCAAAAAAGGGCTAGTA+AGG - chr8_2:65112361-65112380 None:intergenic 40.0%
CATGGAACTATAGCCAAAAG+GGG - chr8_2:65112417-65112436 None:intergenic 40.0%
CTCATGGAACTATAGCCAAA+AGG - chr8_2:65112419-65112438 None:intergenic 40.0%
ACTCAGTAACTGCATTCTCA+TGG - chr8_2:65112435-65112454 None:intergenic 40.0%
AGAATGCAGTTACTGAGTCT+TGG + chr8_2:65112437-65112456 Msa1262420:three_prime_UTR 40.0%
AGTTACTGAGTCTTGGTTGA+TGG + chr8_2:65112444-65112463 Msa1262420:three_prime_UTR 40.0%
GTTAACCTCCTTGTAAGGCA+AGG + chr8_2:65109168-65109187 Msa1262420:CDS 45.0%
! GAAAGTGGTATTTGCTGAGG+TGG + chr8_2:65109320-65109339 Msa1262420:intron 45.0%
! CTGTTTCTTGTGTTCATGCC+TGG + chr8_2:65109728-65109747 Msa1262420:intron 45.0%
AGAGCAGTGCACATCAACAT+TGG - chr8_2:65110352-65110371 None:intergenic 45.0%
!! CTGATTCTAGTTCGCTTCGT+AGG - chr8_2:65110375-65110394 None:intergenic 45.0%
ATATTCAGACGGCTTCCAAG+TGG + chr8_2:65110492-65110511 Msa1262420:CDS 45.0%
CAGGTGCCTGTGTATCAAAA+AGG + chr8_2:65110529-65110548 Msa1262420:CDS 45.0%
GTGCCTGTGTATCAAAAAGG+TGG + chr8_2:65110532-65110551 Msa1262420:CDS 45.0%
GAGAGTGATGAAGATGCTGA+AGG + chr8_2:65110621-65110640 Msa1262420:CDS 45.0%
GATGAAGATGCTGAAGGTGA+TGG + chr8_2:65110627-65110646 Msa1262420:CDS 45.0%
! AAGGTGATGGCTACCTTACA+AGG + chr8_2:65110640-65110659 Msa1262420:CDS 45.0%
! TACCTTACAAGGCGTTTGGA+AGG + chr8_2:65110651-65110670 Msa1262420:CDS 45.0%
! ACCTTACAAGGCGTTTGGAA+GGG + chr8_2:65110652-65110671 Msa1262420:CDS 45.0%
ATAGCTTTCCAAGTCCACCA+CGG - chr8_2:65110728-65110747 None:intergenic 45.0%
CTCTCAGATCACTTTCACTG+TGG + chr8_2:65110820-65110839 Msa1262420:CDS 45.0%
!! TGTTGGTGATCGACGAAGAA+TGG - chr8_2:65110923-65110942 None:intergenic 45.0%
TTCTTCGTCGATCACCAACA+AGG + chr8_2:65110923-65110942 Msa1262420:CDS 45.0%
TCTTCGTCGATCACCAACAA+GGG + chr8_2:65110924-65110943 Msa1262420:CDS 45.0%
TGTCCTCTTGTTGGAGATCA+TGG + chr8_2:65111080-65111099 Msa1262420:CDS 45.0%
ACACCATGATCTCCAACAAG+AGG - chr8_2:65111086-65111105 None:intergenic 45.0%
TTCGGCTTCCAACTTGTCAA+AGG + chr8_2:65111121-65111140 Msa1262420:CDS 45.0%
CTGGCTGTTATTGGCCATTA+TGG + chr8_2:65111179-65111198 Msa1262420:CDS 45.0%
TGGAAAGGCAGTAACGAATC+TGG + chr8_2:65111199-65111218 Msa1262420:CDS 45.0%
! GAAAGAGGGTTTTGCGTCAT+GGG + chr8_2:65111248-65111267 Msa1262420:CDS 45.0%
CTTTGTATCCGACATTGGAC+TGG + chr8_2:65111400-65111419 Msa1262420:CDS 45.0%
AAATGCTGCCAGTCCAATGT+CGG - chr8_2:65111411-65111430 None:intergenic 45.0%
CTGGAGAGATCTTGTGCATT+TGG - chr8_2:65111435-65111454 None:intergenic 45.0%
GGGCATAGAAAATCCCACAT+TGG - chr8_2:65111490-65111509 None:intergenic 45.0%
CTTCAGCATGTTGATCTGAC+TGG + chr8_2:65111689-65111708 Msa1262420:CDS 45.0%
GTTGATCTGACTGGACTTTG+CGG + chr8_2:65111698-65111717 Msa1262420:CDS 45.0%
!! CCCTCTTTTGGAGAATTGTG+AGG + chr8_2:65111742-65111761 Msa1262420:CDS 45.0%
GCCTCACAATTCTCCAAAAG+AGG - chr8_2:65111746-65111765 None:intergenic 45.0%
!! CTTTTGGAGAATTGTGAGGC+TGG + chr8_2:65111746-65111765 Msa1262420:CDS 45.0%
ACATAGTTTCCATGTTGGCC+AGG + chr8_2:65111813-65111832 Msa1262420:CDS 45.0%
AAGTGTGCAATCACTGATGC+AGG + chr8_2:65111950-65111969 Msa1262420:CDS 45.0%
ATGATCAGCACGAGAGAGAA+CGG - chr8_2:65111981-65112000 None:intergenic 45.0%
CTCTCTATGTCTGACTGTTC+AGG + chr8_2:65112016-65112035 Msa1262420:CDS 45.0%
GTGCCTTTCCTGGTGAAATT+AGG + chr8_2:65112055-65112074 Msa1262420:CDS 45.0%
TGGCCTAATTTCACCAGGAA+AGG - chr8_2:65112061-65112080 None:intergenic 45.0%
TTTCCTGGTGAAATTAGGCC+AGG + chr8_2:65112060-65112079 Msa1262420:CDS 45.0%
ATATTCTGGCCTAATCGCGA+TGG + chr8_2:65112167-65112186 Msa1262420:exon 45.0%
!!! GTTGACTCGGTTGTTTTCCA+AGG + chr8_2:65112320-65112339 Msa1262420:three_prime_UTR 45.0%
ATGAAATTGGGCCGTTGAGG+AGG - chr8_2:65109205-65109224 None:intergenic 50.0%
! GTGGTATTTGCTGAGGTGGT+TGG + chr8_2:65109324-65109343 Msa1262420:intron 50.0%
ATGAATTGCATCCTGGGGGT+AGG + chr8_2:65110310-65110329 Msa1262420:CDS 50.0%
TGAATTGCATCCTGGGGGTA+GGG + chr8_2:65110311-65110330 Msa1262420:CDS 50.0%
GCCTACCATTGAGATCCTTC+CGG + chr8_2:65110452-65110471 Msa1262420:CDS 50.0%
TCCGGAAGGATCTCAATGGT+AGG - chr8_2:65110456-65110475 None:intergenic 50.0%
TTCGTCCGGAAGGATCTCAA+TGG - chr8_2:65110460-65110479 None:intergenic 50.0%
CAAAGAGACATTCGTCCGGA+AGG - chr8_2:65110470-65110489 None:intergenic 50.0%
GAGCTTCTCTCTTTGCCACT+TGG - chr8_2:65110510-65110529 None:intergenic 50.0%
AGTGGCAAAGAGAGAAGCTC+AGG + chr8_2:65110510-65110529 Msa1262420:CDS 50.0%
! TGGCTACCTTACAAGGCGTT+TGG + chr8_2:65110647-65110666 Msa1262420:CDS 50.0%
TCCCTTCCAAACGCCTTGTA+AGG - chr8_2:65110656-65110675 None:intergenic 50.0%
! TTACAAGGCGTTTGGAAGGG+AGG + chr8_2:65110655-65110674 Msa1262420:CDS 50.0%
GGAGGAAAGCCACTGATGTA+AGG + chr8_2:65110673-65110692 Msa1262420:CDS 50.0%
AGCAGCAAGCCTTACATCAG+TGG - chr8_2:65110685-65110704 None:intergenic 50.0%
!! AGGCTTGCTGCTATTGCTGT+TGG + chr8_2:65110693-65110712 Msa1262420:CDS 50.0%
!! GGCTTGCTGCTATTGCTGTT+GGG + chr8_2:65110694-65110713 Msa1262420:CDS 50.0%
! AGAGGAAACAACTCGACTCG+AGG + chr8_2:65110753-65110772 Msa1262420:CDS 50.0%
! CGAAAGAGGGTTTTGCGTCA+TGG + chr8_2:65111247-65111266 Msa1262420:CDS 50.0%
! AGCATCGAAGCCATGGGTAA+AGG + chr8_2:65111338-65111357 Msa1262420:CDS 50.0%
GTGTGGGAAACCTTTACCCA+TGG - chr8_2:65111351-65111370 None:intergenic 50.0%
ATCTCTCCAGAGCTTGCAGT+TGG + chr8_2:65111445-65111464 Msa1262420:CDS 50.0%
ACTCTTCCAACTGCAAGCTC+TGG - chr8_2:65111454-65111473 None:intergenic 50.0%
TTGTGAGGCTGGACTTGTCA+AGG + chr8_2:65111757-65111776 Msa1262420:CDS 50.0%
CTTGTCAAGGTGAACCTTGC+AGG + chr8_2:65111770-65111789 Msa1262420:CDS 50.0%
TCAAGTTCCAGCAACCTGCA+AGG - chr8_2:65111787-65111806 None:intergenic 50.0%
GAACAAGTGTGTGCCTTTCC+TGG + chr8_2:65112045-65112064 Msa1262420:CDS 50.0%
! GAAATTAGGCCAGGCGTTGT+CGG + chr8_2:65112069-65112088 Msa1262420:CDS 50.0%
! AAATTAGGCCAGGCGTTGTC+GGG + chr8_2:65112070-65112089 Msa1262420:CDS 50.0%
TCAGCAGTTCCATCGCGATT+AGG - chr8_2:65112179-65112198 None:intergenic 50.0%
CGCGATGGAACTGCTGAATA+AGG + chr8_2:65112182-65112201 Msa1262420:three_prime_UTR 50.0%
AGGCAATGTGCGATCTAGAG+TGG - chr8_2:65112473-65112492 None:intergenic 50.0%
ACTCTAGATCGCACATTGCC+TGG + chr8_2:65112472-65112491 Msa1262420:three_prime_UTR 50.0%
CTCTAGATCGCACATTGCCT+GGG + chr8_2:65112473-65112492 Msa1262420:three_prime_UTR 50.0%
ATAGTGTACACCCTACCCCC+AGG - chr8_2:65110324-65110343 None:intergenic 55.0%
AGCTCGCTGAAGAGCTCCAA+AGG - chr8_2:65110409-65110428 None:intergenic 55.0%
CTTCAGCGAGCTCTTGAGCA+CGG + chr8_2:65110417-65110436 Msa1262420:CDS 55.0%
TTCAGCGAGCTCTTGAGCAC+GGG + chr8_2:65110418-65110437 Msa1262420:CDS 55.0%
! GCTGCTATTGCTGTTGGGAC+TGG + chr8_2:65110699-65110718 Msa1262420:CDS 55.0%
! ACTCGAGGTGTTACAGACCG+CGG + chr8_2:65110768-65110787 Msa1262420:CDS 55.0%
GGTCTCTCAGCAGTTGCTCA+TGG + chr8_2:65110789-65110808 Msa1262420:CDS 55.0%
TGCGTCATGGGAGTTGCTCA+AGG + chr8_2:65111260-65111279 Msa1262420:CDS 55.0%
CATGGCTTCGATGCTCGCAT+CGG - chr8_2:65111333-65111352 None:intergenic 55.0%
!! GATGCGAGCATCGAAGCCAT+GGG + chr8_2:65111332-65111351 Msa1262420:CDS 55.0%
GGCACATCTGCTTCAGGTGT+GGG - chr8_2:65111367-65111386 None:intergenic 55.0%
AGGCACATCTGCTTCAGGTG+TGG - chr8_2:65111368-65111387 None:intergenic 55.0%
! TGCGAAGGCACATCTGCTTC+AGG - chr8_2:65111373-65111392 None:intergenic 55.0%
ATGCTAGCACTGCCGAAACC+AGG - chr8_2:65111641-65111660 None:intergenic 55.0%
TGGTTTCGGCAGTGCTAGCA+TGG + chr8_2:65111640-65111659 Msa1262420:CDS 55.0%
AGTGCTAGCATGGCTGTGGT+TGG + chr8_2:65111650-65111669 Msa1262420:CDS 55.0%
GTGCTAGCATGGCTGTGGTT+GGG + chr8_2:65111651-65111670 Msa1262420:CDS 55.0%
CTTTGCGGCATAACTGACGC+TGG + chr8_2:65111713-65111732 Msa1262420:CDS 55.0%
AGGTGAACCTTGCAGGTTGC+TGG + chr8_2:65111777-65111796 Msa1262420:CDS 55.0%
TCCATGTTGGCCAGGCTACA+TGG + chr8_2:65111821-65111840 Msa1262420:CDS 55.0%
ACCATGTAGCCTGGCCAACA+TGG - chr8_2:65111825-65111844 None:intergenic 55.0%
ATGTTGGCCAGGCTACATGG+TGG + chr8_2:65111824-65111843 Msa1262420:CDS 55.0%
GAGAGTTCCACCATGTAGCC+TGG - chr8_2:65111834-65111853 None:intergenic 55.0%
ACGCCTGGCCTAATTTCACC+AGG - chr8_2:65112066-65112085 None:intergenic 55.0%
TATTCAAGCCCGACAACGCC+TGG - chr8_2:65112081-65112100 None:intergenic 55.0%
TCAAGTGTGGATTTGCGGCG+CGG + chr8_2:65112294-65112313 Msa1262420:three_prime_UTR 55.0%
CCACTCTGATTGACACGGCC+AGG - chr8_2:65109749-65109768 None:intergenic 60.0%
CCTGGCCGTGTCAATCAGAG+TGG + chr8_2:65109746-65109765 Msa1262420:intron 60.0%
!! ACTGGTTGCCGTGGTGGACT+TGG + chr8_2:65110717-65110736 Msa1262420:CDS 60.0%
GAGCAACTGCTGAGAGACCG+CGG - chr8_2:65110788-65110807 None:intergenic 60.0%
!! TGCTCGCATCGGTTACCCCT+TGG - chr8_2:65111322-65111341 None:intergenic 60.0%
!! CGATGCGAGCATCGAAGCCA+TGG + chr8_2:65111331-65111350 Msa1262420:CDS 60.0%
!!! CGCTGGCCTTCTCCCTCTTT+TGG + chr8_2:65111730-65111749 Msa1262420:CDS 60.0%
!! TGCGGCGCGGTTTGTTGACT+CGG + chr8_2:65112307-65112326 Msa1262420:three_prime_UTR 60.0%
!! GCTGTTGGGACTGGTTGCCG+TGG + chr8_2:65110708-65110727 Msa1262420:CDS 65.0%
!! GTTGGGACTGGTTGCCGTGG+TGG + chr8_2:65110711-65110730 Msa1262420:CDS 65.0%
!! CGCATCGGTTACCCCTTGGC+AGG - chr8_2:65111318-65111337 None:intergenic 65.0%
CGGCAGTGCTAGCATGGCTG+TGG + chr8_2:65111646-65111665 Msa1262420:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 65109165 65112620 65109165 ID=Msa1262420;Name=Msa1262420
chr8_2 mRNA 65109165 65112620 65109165 ID=Msa1262420-mRNA-1;Parent=Msa1262420;Name=Msa1262420-mRNA-1;_AED=0.10;_eAED=0.13;_QI=0|0|0.5|1|1|1|2|440|647
chr8_2 exon 65109165 65109231 65109165 ID=Msa1262420-mRNA-1:exon:21085;Parent=Msa1262420-mRNA-1
chr8_2 exon 65110304 65112620 65110304 ID=Msa1262420-mRNA-1:exon:21086;Parent=Msa1262420-mRNA-1
chr8_2 CDS 65109165 65109231 65109165 ID=Msa1262420-mRNA-1:cds;Parent=Msa1262420-mRNA-1
chr8_2 CDS 65110304 65112180 65110304 ID=Msa1262420-mRNA-1:cds;Parent=Msa1262420-mRNA-1
chr8_2 three_prime_UTR 65112181 65112620 65112181 ID=Msa1262420-mRNA-1:three_prime_utr;Parent=Msa1262420-mRNA-1
Gene Sequence

>Msa1262420

ATGGTTAACCTCCTTGTAAGGCAAGGTTATTCAACAACCTCCTCAACGGCCCAATTTCATAAAAAAGGTAATGATGAATTGCATCCTGGGGGTAGGGTGTACACTATTAGTTCCAATGTTGATGTGCACTGCTCTCCTACGAAGCGAACTAGAATCAGTGCCTCATTTACCTTTGGAGCTCTTCAGCGAGCTCTTGAGCACGGGCAAGATCTTAAGCCTACCATTGAGATCCTTCCGGACGAATGTCTCTTTGAGATATTCAGACGGCTTCCAAGTGGCAAAGAGAGAAGCTCAGGTGCCTGTGTATCAAAAAGGTGGCTTATGCTTATGAGCAGTATCTGCAAAGATGACATTGAATCTGATGTAGAAACAATCTCTTCAGATGAGAGTGATGAAGATGCTGAAGGTGATGGCTACCTTACAAGGCGTTTGGAAGGGAGGAAAGCCACTGATGTAAGGCTTGCTGCTATTGCTGTTGGGACTGGTTGCCGTGGTGGACTTGGAAAGCTATCTATTAGAGGAAACAACTCGACTCGAGGTGTTACAGACCGCGGTCTCTCAGCAGTTGCTCATGGTTGCCCTTCTCTCAGATCACTTTCACTGTGGAATGTGTCTTCTATTGGTGATAAGGGTCTTTGTGAGATTGCAAAAGGATGTCATATGTTGGAGACGCTCGACTTAAGCCATTCTTCGTCGATCACCAACAAGGGTTTGATTGCAATAGCTGAAGGTTGCCCTAACTTGACAACCTTAAACATTGAATCATGCTCGATGATTGGAAATGAGGGTCTGCAAGCTGTTGCAAAGTTATGTCCCAAGTTACATTCAATCTGTATCAAGGATTGTCCTCTTGTTGGAGATCATGGTGTGTCTTGTTTGCTATATTCGGCTTCCAACTTGTCAAAGGTAAAGCTTCAGATTTTGAATATCACTGATTTTTCTCTGGCTGTTATTGGCCATTATGGAAAGGCAGTAACGAATCTGGTTCTAAGTGGTCTTAAAAATGTAAGCGAAAGAGGGTTTTGCGTCATGGGAGTTGCTCAAGGTCTACAGAAATTGATGTCACTAACTATTACTTCCTGCCAAGGGGTAACCGATGCGAGCATCGAAGCCATGGGTAAAGGTTTCCCACACCTGAAGCAGATGTGCCTTCGCAGATGTAGCTTTGTATCCGACATTGGACTGGCAGCATTTGCCAAATGCACAAGATCTCTCCAGAGCTTGCAGTTGGAAGAGTGTAACAGATTCACCCAATGTGGGATTTTCTATGCCCTTTCAAACATCAAAACGAAGTTGAAATCATTTACACTTGTGAAGTGTATGGGAATTAAAGACATTGATGTGGAAGTATCTATGCTTTCACCTTGCAAGTCTCTTCGATCTTTAACCATTCAAAACTGTCCTGGTTTCGGCAGTGCTAGCATGGCTGTGGTTGGGAAATTGTGTCCCCAACTTCAGCATGTTGATCTGACTGGACTTTGCGGCATAACTGACGCTGGCCTTCTCCCTCTTTTGGAGAATTGTGAGGCTGGACTTGTCAAGGTGAACCTTGCAGGTTGCTGGAACTTGACAGATTACATAGTTTCCATGTTGGCCAGGCTACATGGTGGAACTCTCGAAATACTAAACCTCGATGGATGCCAGAATATTACTGATGCAAGTTTGGCTGCAGTTGCAGACGACTGCTTCCTGTTGAATGATCTAGATGTCTCAAAGTGTGCAATCACTGATGCAGGCATAGCCGTTCTCTCTCGTGCTGATCATCTTAGTATGCGAGTGCTCTCTATGTCTGACTGTTCAGGTATATCGAACAAGTGTGTGCCTTTCCTGGTGAAATTAGGCCAGGCGTTGTCGGGCTTGAATATTAAAAATTGCAATACAATTGACAGCAATGCAATTGAGTTTCTCGTGGAGAATTTATGGAGATGTGATATTCTGGCCTAA
Protein sequence

>Msa1262420

MVNLLVRQGYSTTSSTAQFHKKGNDELHPGGRVYTISSNVDVHCSPTKRTRISASFTFGALQRALEHGQDLKPTIEILPDECLFEIFRRLPSGKERSSGACVSKRWLMLMSSICKDDIESDVETISSDESDEDAEGDGYLTRRLEGRKATDVRLAAIAVGTGCRGGLGKLSIRGNNSTRGVTDRGLSAVAHGCPSLRSLSLWNVSSIGDKGLCEIAKGCHMLETLDLSHSSSITNKGLIAIAEGCPNLTTLNIESCSMIGNEGLQAVAKLCPKLHSICIKDCPLVGDHGVSCLLYSASNLSKVKLQILNITDFSLAVIGHYGKAVTNLVLSGLKNVSERGFCVMGVAQGLQKLMSLTITSCQGVTDASIEAMGKGFPHLKQMCLRRCSFVSDIGLAAFAKCTRSLQSLQLEECNRFTQCGIFYALSNIKTKLKSFTLVKCMGIKDIDVEVSMLSPCKSLRSLTIQNCPGFGSASMAVVGKLCPQLQHVDLTGLCGITDAGLLPLLENCEAGLVKVNLAGCWNLTDYIVSMLARLHGGTLEILNLDGCQNITDASLAAVADDCFLLNDLDVSKCAITDAGIAVLSRADHLSMRVLSMSDCSGISNKCVPFLVKLGQALSGLNIKNCNTIDSNAIEFLVENLWRCDILA*