AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1214780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1214780 MtrunA17_Chr4g0063841 95.103 633 31 0 1 633 1 633 0.0 1169
Msa1214780 MtrunA17_Chr1g0149351 61.389 619 229 4 21 633 29 643 0.0 739
Msa1214780 MtrunA17_Chr3g0137771 55.814 602 258 3 34 632 44 640 0.0 642
Msa1214780 MtrunA17_Chr4g0063861 82.564 390 68 0 244 633 1 390 0.0 622
Msa1214780 MtrunA17_Chr4g0063861 24.812 399 242 9 167 536 2 371 1.52e-18 88.2
Msa1214780 MtrunA17_Chr4g0063861 23.729 295 190 7 152 414 92 383 4.99e-11 65.1
Msa1214780 MtrunA17_Chr4g0063851 81.921 177 19 3 1 171 1 170 5.84e-91 281
Msa1214780 MtrunA17_Chr2g0286991 25.990 581 367 16 64 597 11 575 8.29e-40 154
Msa1214780 MtrunA17_Chr2g0286991 26.407 462 300 10 167 625 153 577 2.65e-35 141
Msa1214780 MtrunA17_Chr2g0286991 27.873 409 283 11 219 625 127 525 4.34e-25 110
Msa1214780 MtrunA17_Chr2g0286991 29.856 278 160 5 144 418 340 585 6.72e-24 106
Msa1214780 MtrunA17_Chr2g0286991 28.435 313 210 6 167 474 281 584 5.20e-22 100
Msa1214780 MtrunA17_Chr7g0272131 28.764 445 282 11 154 586 184 605 8.23e-30 125
Msa1214780 MtrunA17_Chr7g0272131 26.496 468 274 14 182 620 135 561 2.80e-21 98.6
Msa1214780 MtrunA17_Chr7g0272131 26.263 297 186 6 165 460 399 663 3.20e-19 92.0
Msa1214780 MtrunA17_Chr4g0004901 27.021 470 299 16 129 583 179 619 8.32e-26 112
Msa1214780 MtrunA17_Chr4g0004901 26.250 480 316 15 167 619 135 603 9.45e-25 109
Msa1214780 MtrunA17_Chr4g0004901 29.204 339 203 10 166 503 366 668 2.54e-21 98.6
Msa1214780 MtrunA17_Chr1g0212071 24.706 425 277 11 62 478 18 407 1.18e-25 110
Msa1214780 MtrunA17_Chr1g0212071 25.587 426 280 11 95 508 10 410 1.23e-17 85.9
Msa1214780 MtrunA17_Chr1g0212071 23.469 294 195 6 349 614 94 385 3.37e-16 81.3
Msa1214780 MtrunA17_Chr1g0212071 31.122 196 131 4 428 622 94 286 5.94e-16 80.5
Msa1214780 MtrunA17_Chr8g0347711 27.174 368 223 10 60 403 11 357 4.21e-20 92.8
Msa1214780 MtrunA17_Chr3g0125671 31.183 186 102 2 201 386 123 282 5.94e-18 86.3
Msa1214780 MtrunA17_Chr3g0125671 32.877 146 71 3 134 255 140 282 6.87e-11 64.3
Msa1214780 MtrunA17_Chr4g0008501 22.625 579 392 21 49 597 30 582 7.38e-17 84.3
Msa1214780 MtrunA17_Chr1g0176031 30.204 245 153 5 165 399 90 326 8.16e-16 79.3
Msa1214780 MtrunA17_Chr1g0176031 21.321 333 198 8 180 506 52 326 1.05e-14 75.9
Msa1214780 MtrunA17_Chr1g0176031 29.918 244 160 8 335 577 107 340 1.49e-14 75.5
Msa1214780 MtrunA17_Chr1g0150971 24.883 426 245 15 179 583 339 710 8.82e-16 81.3
Msa1214780 MtrunA17_Chr1g0152951 24.444 360 207 10 215 570 134 432 1.13e-13 73.9
Msa1214780 MtrunA17_Chr1g0152951 37.190 121 72 3 168 286 320 438 5.22e-11 65.5
Msa1214780 MtrunA17_Chr4g0070541 26.744 258 181 7 336 590 397 649 1.14e-13 74.3
Msa1214780 MtrunA17_Chr1g0159531 33.333 153 95 3 134 285 128 274 1.61e-13 72.4
Msa1214780 MtrunA17_Chr1g0159531 28.505 214 141 3 164 365 100 313 8.11e-13 70.5
Msa1214780 MtrunA17_Chr4g0018891 25.532 329 208 13 219 545 189 482 6.32e-12 68.2
Msa1214780 MtrunA17_Chr8g0352431 23.965 459 286 17 171 597 131 558 8.79e-12 68.2
Msa1214780 MtrunA17_Chr8g0342371 23.649 444 307 15 171 597 136 564 1.03e-11 67.8
Msa1214780 MtrunA17_Chr1g0150991 23.472 409 246 15 179 583 155 500 1.62e-11 67.4
Msa1214780 MtrunA17_Chr2g0300611 24.324 259 164 5 360 590 400 654 1.74e-11 67.4
Msa1214780 MtrunA17_Chr3g0079141 22.652 543 312 21 23 544 11 466 2.44e-11 66.6
Msa1214780 MtrunA17_Chr4g0070521 24.254 268 185 7 336 590 28 290 2.84e-11 65.5
Msa1214780 MtrunA17_Chr5g0436511 25.526 380 210 18 166 506 145 490 3.71e-11 66.2
Msa1214780 MtrunA17_Chr7g0251831 28.780 205 121 9 187 374 315 511 4.71e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1214780 AT2G25490.1 51.724 638 279 7 8 633 8 628 0.0 642
Msa1214780 AT5G25350.1 52.276 637 284 7 1 632 1 622 0.0 606
Msa1214780 AT4G15475.1 28.328 586 347 19 64 597 11 575 3.69e-43 164
Msa1214780 AT4G15475.1 27.378 347 214 6 129 474 275 584 1.25e-27 118
Msa1214780 AT4G15475.1 26.575 365 232 7 167 531 281 609 1.65e-23 105
Msa1214780 AT4G15475.1 24.118 340 223 9 309 619 109 442 3.37e-16 82.8
Msa1214780 AT4G15475.1 30.769 156 103 3 143 298 443 593 6.26e-12 68.9
Msa1214780 AT5G27920.1 24.665 523 332 12 151 621 90 602 4.36e-28 120
Msa1214780 AT5G27920.1 29.268 246 168 5 167 411 364 604 1.73e-17 87.0
Msa1214780 AT5G23340.1 24.941 421 280 8 62 478 8 396 6.12e-24 105
Msa1214780 AT5G23340.1 24.684 316 207 4 296 610 85 370 3.53e-21 96.7
Msa1214780 AT5G23340.1 31.658 199 132 4 428 625 84 279 7.14e-19 89.7
Msa1214780 AT5G01720.1 26.200 500 298 17 167 610 85 569 1.81e-22 102
Msa1214780 AT5G01720.1 23.604 394 236 5 166 536 238 589 1.49e-20 96.7
Msa1214780 AT5G01720.1 20.924 368 236 9 162 505 260 596 4.48e-11 66.6
Msa1214780 AT5G01720.1 29.412 170 89 2 192 336 443 606 4.72e-11 66.2
Msa1214780 AT1G21410.2 28.351 194 113 2 193 386 104 271 6.43e-17 83.2
Msa1214780 AT1G21410.2 27.004 237 110 5 167 376 130 330 8.27e-14 73.9
Msa1214780 AT1G21410.1 28.351 194 113 2 193 386 104 271 6.43e-17 83.2
Msa1214780 AT1G21410.1 27.004 237 110 5 167 376 130 330 8.27e-14 73.9
Msa1214780 AT3G58530.1 27.132 258 154 7 145 399 102 328 1.83e-15 79.0
Msa1214780 AT3G58530.1 21.557 334 197 7 180 506 53 328 1.24e-13 73.2
Msa1214780 AT3G58530.1 25.000 244 154 5 390 608 112 351 1.09e-11 67.4
Msa1214780 AT1G47056.1 25.962 416 216 12 192 604 116 442 3.30e-15 79.3
Msa1214780 AT1G47056.1 33.918 171 102 5 124 292 260 421 1.61e-13 73.9
Msa1214780 AT1G80630.1 21.981 414 275 10 181 559 108 508 5.59e-15 78.6
Msa1214780 AT1G77000.2 28.947 228 143 5 167 376 104 330 2.77e-14 75.5
Msa1214780 AT1G77000.1 28.947 228 143 5 167 376 104 330 2.77e-14 75.5
Msa1214780 AT3G07550.1 23.098 368 239 8 52 408 6 340 2.27e-13 72.8
Msa1214780 AT3G07550.2 23.098 368 239 8 52 408 6 340 2.27e-13 72.8
Msa1214780 AT3G07550.3 24.167 360 218 8 52 408 6 313 5.01e-13 71.6
Msa1214780 AT4G30640.1 29.808 208 127 5 51 256 10 200 2.04e-12 68.9
Msa1214780 AT1G77000.3 28.324 173 98 2 193 365 104 250 1.44e-11 65.9
Msa1214780 AT1G77000.4 28.324 173 98 2 193 365 104 250 1.44e-11 65.9
Msa1214780 AT5G67250.1 23.913 368 196 10 216 570 117 413 4.31e-11 66.2
Msa1214780 AT4G07400.1 37.008 127 76 3 168 292 330 454 6.85e-11 65.9

Find 196 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGCATGATTCAATGTTTA+AGG 0.123906 8_1:-75559769 None:intergenic
CGCTGGCCTTCTCCCTCTTT+TGG 0.142205 8_1:+75560514 Msa1214780:CDS
AGTTTCTCGTGGAGAATTTA+TGG 0.177171 8_1:+75560921 Msa1214780:CDS
CTGGCTGTTATTGGCCATTA+TGG 0.192129 8_1:+75559963 Msa1214780:CDS
TGAAGAGCTCCAAAGGTAAA+TGG 0.215306 8_1:-75559183 None:intergenic
CGCGATGGAACTGCTGAATA+AGG 0.264291 8_1:+75560966 Msa1214780:three_prime_UTR
ATGTTTAAGGTTGTCAAGTT+AGG 0.283107 8_1:-75559756 None:intergenic
AGAAATATAAAGAGTGGTTT+TGG 0.296107 8_1:+75558039 Msa1214780:five_prime_UTR
TCAATATCAAAGTACAAATA+TGG 0.313413 8_1:+75557896 Msa1214780:five_prime_UTR
TGTTATATTCTGTTTGTTGC+AGG 0.317403 8_1:+75561301 Msa1214780:three_prime_UTR
TGTGTCTTGTTTGCTATATT+CGG 0.319855 8_1:+75559887 Msa1214780:CDS
TCGTTACTGCCTTTCCATAA+TGG 0.327153 8_1:-75559977 None:intergenic
GAATATTACTGATGCAAGTT+TGG 0.327338 8_1:+75560664 Msa1214780:CDS
TTTATGGAGATGTGATATTC+TGG 0.332414 8_1:+75560937 Msa1214780:CDS
CTGGAGAGATCTTGTGCATT+TGG 0.332649 8_1:-75560216 None:intergenic
CTCTCTATGTCTGACTGTTC+AGG 0.334066 8_1:+75560800 Msa1214780:CDS
AACTTGCATCAGTAATATTC+TGG 0.336340 8_1:-75560661 None:intergenic
TGTCCTCTTGTTGGAGATCA+TGG 0.346783 8_1:+75559864 Msa1214780:CDS
ATTCAAAACTGTCCTGGTTT+CGG 0.358584 8_1:+75560410 Msa1214780:CDS
CTGTTTCTTGTGCTCATGCC+TGG 0.359324 8_1:+75558478 Msa1214780:exon
CTTGTCAAGGTGAACCTTGC+AGG 0.363717 8_1:+75560554 Msa1214780:CDS
AGGTGAACCTTGCAGGTTGC+TGG 0.365852 8_1:+75560561 Msa1214780:CDS
GCTGAATAAGGAAATGCTAA+TGG 0.368749 8_1:+75560978 Msa1214780:three_prime_UTR
GTGCCTTTCCTGGTGAAATT+AGG 0.368798 8_1:+75560839 Msa1214780:CDS
GTTGATCTGACTGGACTTTG+TGG 0.370762 8_1:+75560482 Msa1214780:CDS
ACTCTAGATCGCACATTGCC+TGG 0.374175 8_1:+75561256 Msa1214780:three_prime_UTR
TGCGAAGGCACATCTGCTTC+AGG 0.374917 8_1:-75560154 None:intergenic
ATTTACACTTGTGAAGTGTA+TGG 0.378579 8_1:+75560322 Msa1214780:CDS
GGCTTGCTGCTATTGCTGTT+GGG 0.383869 8_1:+75559478 Msa1214780:CDS
ATCAGTGCCCCATTTACCTT+TGG 0.383910 8_1:+75559174 Msa1214780:CDS
GAAATTAGGCCAGGCGTTGT+CGG 0.389515 8_1:+75560853 Msa1214780:CDS
GCTGCTATTGCTGTTGGGAC+TGG 0.389778 8_1:+75559483 Msa1214780:CDS
CTGTGGAATGTGTCTTCTAT+TGG 0.391657 8_1:+75559621 Msa1214780:CDS
GAAGAGCTCCAAAGGTAAAT+GGG 0.401450 8_1:-75559182 None:intergenic
TGTGTCTTCTATTGGTGATA+AGG 0.403727 8_1:+75559629 Msa1214780:CDS
ACCATGTAGCCTGGCCAACT+TGG 0.404543 8_1:-75560606 None:intergenic
AGAGGTATATAACAGAGAAA+AGG 0.404807 8_1:-75558388 None:intergenic
ACTGGTTGCCGTGGTGGACT+TGG 0.405676 8_1:+75559501 Msa1214780:CDS
GTGTCTTCTATTGGTGATAA+GGG 0.408571 8_1:+75559630 Msa1214780:CDS
ATTGAATCATGCTCTATGAT+TGG 0.408866 8_1:+75559777 Msa1214780:CDS
GAACAAGTGTGTGCCTTTCC+TGG 0.410656 8_1:+75560829 Msa1214780:CDS
CAGATCAACATGCTGAAGTT+GGG 0.413419 8_1:-75560468 None:intergenic
TCCCTTCCAAACGCCTTGTA+AGG 0.414436 8_1:-75559437 None:intergenic
TCAGCAGTTCCATCGCGATT+AGG 0.414562 8_1:-75560960 None:intergenic
AGGCTTGCTGCTATTGCTGT+TGG 0.416928 8_1:+75559477 Msa1214780:CDS
CTTGGAAAGCTATCTATTAG+AGG 0.419353 8_1:+75559519 Msa1214780:CDS
TGGCTACCTTACAAGGCGTT+TGG 0.422778 8_1:+75559431 Msa1214780:CDS
TCATGGAACTATAGCCAAAA+GGG 0.423795 8_1:-75561199 None:intergenic
GAGGTATATAACAGAGAAAA+GGG 0.425382 8_1:-75558387 None:intergenic
GAGCTTCTCTCTTTGCCACT+TGG 0.430900 8_1:-75559291 None:intergenic
CTCATGGAACTATAGCCAAA+AGG 0.433792 8_1:-75561200 None:intergenic
TGTTTAAGGTTGTCAAGTTA+GGG 0.434609 8_1:-75559755 None:intergenic
ATCAAGGATTGTCCTCTTGT+TGG 0.435290 8_1:+75559855 Msa1214780:CDS
GGTAATGATGAATTGCATCC+TGG 0.437251 8_1:+75559087 Msa1214780:intron
TGCGGCGCGGTTTGTTGACT+CGG 0.438068 8_1:+75561091 Msa1214780:three_prime_UTR
TATTGCAATCAAACCCTTGT+TGG 0.439489 8_1:-75559721 None:intergenic
GACTATCAAGTGTGGATTTG+CGG 0.442563 8_1:+75561073 Msa1214780:three_prime_UTR
TGTTGGTGATCGACGAAGAA+TGG 0.444320 8_1:-75559704 None:intergenic
AAATTAGGCCAGGCGTTGTC+GGG 0.445281 8_1:+75560854 Msa1214780:CDS
ATTTGTACTTTGATATTGAC+CGG 0.448862 8_1:-75557892 None:intergenic
TGGAAAGGCAGTAACGAATC+TGG 0.449057 8_1:+75559983 Msa1214780:CDS
GCCTACCATTGAGATCCTTC+CGG 0.449888 8_1:+75559236 Msa1214780:CDS
TTGAATAACCTTGCCTTACA+AGG 0.450924 8_1:-75557926 None:intergenic
TCCAAGTTGGCCAGGCTACA+TGG 0.454321 8_1:+75560605 Msa1214780:CDS
AGTTACTGAGTCTTGGTTGA+TGG 0.456016 8_1:+75561228 Msa1214780:three_prime_UTR
GTTACATTCAATCTGTATCA+AGG 0.456342 8_1:+75559839 Msa1214780:CDS
AAGCTTTACCTTTGACAAGT+TGG 0.456600 8_1:-75559913 None:intergenic
TATTCAAGCCCGACAACGCC+TGG 0.460376 8_1:-75560862 None:intergenic
AGTGGCAAAGAGAGAAGCTC+AGG 0.464804 8_1:+75559294 Msa1214780:CDS
TGTGATATTCAGCTTATACT+TGG 0.466407 8_1:+75558443 Msa1214780:five_prime_UTR
CTCTAGATCGCACATTGCCT+GGG 0.467454 8_1:+75561257 Msa1214780:three_prime_UTR
AGACATCTAGATCATTCAAC+AGG 0.469504 8_1:-75560709 None:intergenic
TCAGATCAACATGCTGAAGT+TGG 0.473467 8_1:-75560469 None:intergenic
GGTCTTTGTGAGATTGCAAA+AGG 0.475591 8_1:+75559651 Msa1214780:CDS
TTAACCATTCAAAACTGTCC+TGG 0.477543 8_1:+75560404 Msa1214780:CDS
GGCATAGAAAATCCCACATT+GGG 0.478588 8_1:-75560270 None:intergenic
ATATGGTTAACCTCCTTGTA+AGG 0.479102 8_1:+75557913 Msa1214780:five_prime_UTR
ACTCTTCCAACTGCAAGCTC+TGG 0.480930 8_1:-75560235 None:intergenic
GATACAGATTGAATGTAACT+TGG 0.482768 8_1:-75559835 None:intergenic
TTCAGCGAGCTCTTGAGCAC+GGG 0.483184 8_1:+75559202 Msa1214780:CDS
CAGGTGCCTGTGTATCAAAA+AGG 0.484862 8_1:+75559313 Msa1214780:CDS
GCCTCACAATTCTCCAAAAG+AGG 0.487248 8_1:-75560527 None:intergenic
ATGCTCTATGATTGGAAATG+AGG 0.488753 8_1:+75559785 Msa1214780:CDS
TTTCCTGGTGAAATTAGGCC+AGG 0.489686 8_1:+75560844 Msa1214780:CDS
GAGAGTTCCACCATGTAGCC+TGG 0.492311 8_1:-75560615 None:intergenic
CTTTGTATCCGACATTGGAC+TGG 0.492327 8_1:+75560184 Msa1214780:CDS
ACATAGTTTCCAAGTTGGCC+AGG 0.504234 8_1:+75560597 Msa1214780:CDS
GGCACATCTGCTTCAGGTGT+GGG 0.504975 8_1:-75560148 None:intergenic
CTTCAGCGAGCTCTTGAGCA+CGG 0.505562 8_1:+75559201 Msa1214780:CDS
TGGTTTCGGCAGTGCTAGCA+TGG 0.505703 8_1:+75560424 Msa1214780:CDS
AGTGCTAGCATGGCTGTGGT+TGG 0.506787 8_1:+75560434 Msa1214780:CDS
TGCTCGCATCGGTTACCCCT+TGG 0.507539 8_1:-75560103 None:intergenic
AGATTACATAGTTTCCAAGT+TGG 0.509911 8_1:+75560592 Msa1214780:CDS
CGCATCGGTTACCCCTTGGC+AGG 0.510702 8_1:-75560099 None:intergenic
AAGTGTGCAATCACTGATGC+AGG 0.512250 8_1:+75560734 Msa1214780:CDS
GGTCTCTCAGCAGTTGCTCA+TGG 0.513210 8_1:+75559573 Msa1214780:CDS
GGAGGAAAGCCACTGATGTA+AGG 0.514553 8_1:+75559457 Msa1214780:CDS
ATCTCTCCAGAGCTTGCAGT+TGG 0.514820 8_1:+75560229 Msa1214780:CDS
TGCGTCATGGGAGTTGCTCA+AGG 0.518086 8_1:+75560044 Msa1214780:CDS
AAGGTGATGGCTACCTTACA+AGG 0.519297 8_1:+75559424 Msa1214780:CDS
GGAAACAAACCAAAACTCCT+TGG 0.519774 8_1:-75561121 None:intergenic
TGCTCTATGATTGGAAATGA+GGG 0.521873 8_1:+75559786 Msa1214780:CDS
GTGCTAGCATGGCTGTGGTT+GGG 0.523567 8_1:+75560435 Msa1214780:CDS
CCACTCTGATTGACACGGCC+AGG 0.524101 8_1:-75558496 None:intergenic
TACCTTACAAGGCGTTTGGA+AGG 0.525492 8_1:+75559435 Msa1214780:CDS
AGCATCGAAGCCATGGGTAA+AGG 0.526861 8_1:+75560122 Msa1214780:CDS
TTTACACTTGTGAAGTGTAT+GGG 0.528626 8_1:+75560323 Msa1214780:CDS
TGTAGCTTTGTATCCGACAT+TGG 0.531778 8_1:+75560179 Msa1214780:CDS
CTTCAGCATGTTGATCTGAC+TGG 0.536194 8_1:+75560473 Msa1214780:CDS
ATGAAATTGGGCCGTTGAGG+AGG 0.536524 8_1:-75557952 None:intergenic
TGCAAAAGGATGTCATATGT+TGG 0.540414 8_1:+75559665 Msa1214780:CDS
GTGGTATTTGCTGAGGTGGT+TGG 0.541778 8_1:+75558074 Msa1214780:five_prime_UTR
CTGATTCTAGTTCGCTTCGT+AGG 0.543276 8_1:-75559156 None:intergenic
GTTGGGACTGGTTGCCGTGG+TGG 0.544249 8_1:+75559495 Msa1214780:CDS
GTAACATTGTACTTATTCTG+TGG 0.544585 8_1:+75558180 Msa1214780:five_prime_UTR
GTAATGATGAATTGCATCCT+GGG 0.545999 8_1:+75559088 Msa1214780:CDS
ATCTCAAAGAGACATTCGTC+CGG 0.551142 8_1:-75559255 None:intergenic
ACTCAGTAACTGCATTCTCA+TGG 0.552061 8_1:-75561216 None:intergenic
GAAAGTGGTATTTGCTGAGG+TGG 0.552266 8_1:+75558070 Msa1214780:five_prime_UTR
TTCGGCTTCCAACTTGTCAA+AGG 0.552626 8_1:+75559905 Msa1214780:CDS
ATGCTAGCACTGCCGAAACC+AGG 0.554879 8_1:-75560422 None:intergenic
CGGCAGTGCTAGCATGGCTG+TGG 0.556009 8_1:+75560430 Msa1214780:CDS
ATTAATAATGAAGCAGCAGA+TGG 0.557908 8_1:+75561018 Msa1214780:three_prime_UTR
ATACAGATTGAATGTAACTT+GGG 0.559199 8_1:-75559834 None:intergenic
AGGCACATCTGCTTCAGGTG+TGG 0.560606 8_1:-75560149 None:intergenic
AGAATGCAGTTACTGAGTCT+TGG 0.560746 8_1:+75561221 Msa1214780:three_prime_UTR
GTGAAAGTGATCTGAGAGAA+GGG 0.561763 8_1:-75559599 None:intergenic
TGGCCTAATTTCACCAGGAA+AGG 0.564441 8_1:-75560842 None:intergenic
ATGATCAGCACGAGAGAGAA+CGG 0.564713 8_1:-75560762 None:intergenic
ACCTTACAAGGCGTTTGGAA+GGG 0.565254 8_1:+75559436 Msa1214780:CDS
AGCTCGCTGAAGAGCTCCAA+AGG 0.565614 8_1:-75559190 None:intergenic
GGTTTGATTGCAATAGCAGA+AGG 0.570491 8_1:+75559729 Msa1214780:CDS
GTTAACCTCCTTGTAAGGCA+AGG 0.573809 8_1:+75557918 Msa1214780:five_prime_UTR
TAACTATTACTTCCTGCCAA+GGG 0.575789 8_1:+75560087 Msa1214780:CDS
TTTATGAAATTGGGCCGTTG+AGG 0.576561 8_1:-75557955 None:intergenic
CTCGAAATACTAAACCTCGA+TGG 0.579655 8_1:+75560635 Msa1214780:CDS
CAAAGAGACATTCGTCCGGA+AGG 0.580269 8_1:-75559251 None:intergenic
GTGTGGGAAACCTTTACCCA+TGG 0.581206 8_1:-75560132 None:intergenic
ATGTTGTTGAATAGATGAAT+CGG 0.584105 8_1:+75557840 None:intergenic
AGCAGCAAGCCTTACATCAG+TGG 0.584954 8_1:-75559466 None:intergenic
GGGCATAGAAAATCCCACAT+TGG 0.585502 8_1:-75560271 None:intergenic
CAATGCAATTGAGTTTCTCG+TGG 0.586036 8_1:+75560910 Msa1214780:CDS
TTCTTCGTCGATCACCAACA+AGG 0.587327 8_1:+75559707 Msa1214780:CDS
AGAGCAGTGCACATCAACAT+TGG 0.587717 8_1:-75559133 None:intergenic
TGTGAAAGTGGTATTTGCTG+AGG 0.587833 8_1:+75558067 Msa1214780:five_prime_UTR
GTCTCTTTGAGATATTCAGA+CGG 0.588078 8_1:+75559265 Msa1214780:CDS
GGGAATTAAAGACATTGATG+TGG 0.591377 8_1:+75560343 Msa1214780:CDS
TTACAAGGCGTTTGGAAGGG+AGG 0.593323 8_1:+75559439 Msa1214780:CDS
CCTCACAATTCTCCAAAAGA+GGG 0.598079 8_1:-75560526 None:intergenic
ATATTCTGGCCTAATCGCGA+TGG 0.598949 8_1:+75560951 Msa1214780:exon
TGTTATTGGCCATTATGGAA+AGG 0.600886 8_1:+75559968 Msa1214780:CDS
CTCTCAGATCACTTTCACTG+TGG 0.601821 8_1:+75559604 Msa1214780:CDS
AGTGAAAGTGATCTGAGAGA+AGG 0.602549 8_1:-75559600 None:intergenic
CATGGCTTCGATGCTCGCAT+CGG 0.603091 8_1:-75560114 None:intergenic
GATGAAGATGCTGAAGGTGA+TGG 0.605262 8_1:+75559411 Msa1214780:CDS
AGGCAATGTGCGATCTAGAG+TGG 0.606144 8_1:-75561254 None:intergenic
TGTAACAGATTCACCCAATG+TGG 0.606794 8_1:+75560257 Msa1214780:CDS
AGATCAACATGCTGAAGTTG+GGG 0.609536 8_1:-75560467 None:intergenic
CTAACTATTACTTCCTGCCA+AGG 0.610927 8_1:+75560086 Msa1214780:CDS
ACACCATGATCTCCAACAAG+AGG 0.615650 8_1:-75559867 None:intergenic
TCTTCGTCGATCACCAACAA+GGG 0.621934 8_1:+75559708 Msa1214780:CDS
GAATAATGAACAAGAAAGAG+AGG 0.622380 8_1:-75558406 None:intergenic
AAATGCTGCCAGTCCAATGT+CGG 0.622406 8_1:-75560192 None:intergenic
AATTCTCCAAAAGAGGGAGA+AGG 0.622832 8_1:-75560520 None:intergenic
GATGCGAGCATCGAAGCCAT+GGG 0.623164 8_1:+75560116 Msa1214780:CDS
TTCGTCCGGAAGGATCTCAA+TGG 0.627959 8_1:-75559241 None:intergenic
ATACAAAGCTACATCTGCGA+AGG 0.636036 8_1:-75560169 None:intergenic
TCAAGTGTGGATTTGCGGCG+CGG 0.636183 8_1:+75561078 Msa1214780:three_prime_UTR
TTATTCAACAACCTCCTCAA+CGG 0.640721 8_1:+75557941 Msa1214780:five_prime_UTR
GTAACGAATCTGGTTCTAAG+TGG 0.640999 8_1:+75559993 Msa1214780:CDS
TAATGATGAATTGCATCCTG+GGG 0.643262 8_1:+75559089 Msa1214780:CDS
GCTGTTGGGACTGGTTGCCG+TGG 0.645340 8_1:+75559492 Msa1214780:CDS
AAGAGCTCCAAAGGTAAATG+GGG 0.648685 8_1:-75559181 None:intergenic
TCAAGTTCCAGCAACCTGCA+AGG 0.650087 8_1:-75560568 None:intergenic
TTGTGAGGCTGGACTTGTCA+AGG 0.655559 8_1:+75560541 Msa1214780:CDS
GCTGATATGACTATCAAGTG+TGG 0.658205 8_1:+75561065 Msa1214780:three_prime_UTR
CTTTGTGGCATAACTGACGC+TGG 0.659354 8_1:+75560497 Msa1214780:CDS
AACTATTACTTCCTGCCAAG+GGG 0.659376 8_1:+75560088 Msa1214780:CDS
GAGCAACTGCTGAGAGACCG+CGG 0.662248 8_1:-75559569 None:intergenic
TAATATTCTGGCATCCATCG+AGG 0.665697 8_1:-75560649 None:intergenic
CATGGAACTATAGCCAAAAG+GGG 0.666185 8_1:-75561198 None:intergenic
GTAACAGATTCACCCAATGT+GGG 0.667862 8_1:+75560258 Msa1214780:CDS
TTGTCATGTAACAAACAAAG+CGG 0.667882 8_1:-75558245 None:intergenic
GAGAGTGATGAAGATGCTGA+AGG 0.668726 8_1:+75559405 Msa1214780:CDS
AATAACCTTGCCTTACAAGG+AGG 0.669394 8_1:-75557923 None:intergenic
TCCGGAAGGATCTCAATGGT+AGG 0.673037 8_1:-75559237 None:intergenic
AGAGGAAACAACTCGACTCG+AGG 0.676030 8_1:+75559537 Msa1214780:CDS
AAAGATCGAAGAGACTTGCA+AGG 0.680643 8_1:-75560383 None:intergenic
AGTGATACAAAGTAAAACCC+AGG 0.687051 8_1:-75561274 None:intergenic
ATATTCAGACGGCTTCCAAG+TGG 0.698602 8_1:+75559276 Msa1214780:CDS
AGTTACCACTCTGATTGACA+CGG 0.709344 8_1:-75558501 None:intergenic
CCTGGCCGTGTCAATCAGAG+TGG 0.712504 8_1:+75558496 Msa1214780:CDS
ACTCGAGGTGTTACAGACCG+CGG 0.719268 8_1:+75559552 Msa1214780:CDS
AAGTTGGCCAGGCTACATGG+TGG 0.728854 8_1:+75560608 Msa1214780:CDS
AATGATGAATTGCATCCTGG+GGG 0.735856 8_1:+75559090 Msa1214780:CDS
ATAGCTTTCCAAGTCCACCA+CGG 0.754154 8_1:-75559509 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTTATATCATTATATTATA+TGG + chr8_1:75558692-75558711 Msa1214780:intron 10.0%
!!! TTTACCTTTTTTATGAAATT+GGG - chr8_1:75557967-75557986 None:intergenic 15.0%
!!! TTTTACCTTTTTTATGAAAT+TGG - chr8_1:75557968-75557987 None:intergenic 15.0%
!! ATAAAAAGAAATATAAAGAG+TGG + chr8_1:75558033-75558052 Msa1214780:five_prime_UTR 15.0%
!!! CATTAAAAAAATCGATTTTA+AGG + chr8_1:75558126-75558145 Msa1214780:five_prime_UTR 15.0%
!!! TTTTTAAGTTTTCTAGTTTA+TGG + chr8_1:75558612-75558631 Msa1214780:intron 15.0%
!! AAGAATAACAAAGAAAATAT+TGG + chr8_1:75558791-75558810 Msa1214780:intron 15.0%
!! TCAATATCAAAGTACAAATA+TGG + chr8_1:75557896-75557915 Msa1214780:five_prime_UTR 20.0%
!!! TTTATGTCTTCAATTGTTTT+GGG - chr8_1:75558018-75558037 None:intergenic 20.0%
!!! TTTTATGTCTTCAATTGTTT+TGG - chr8_1:75558019-75558038 None:intergenic 20.0%
!! GGAAATAGAAAAAAAATCTT+AGG - chr8_1:75558213-75558232 None:intergenic 20.0%
!! ACTAGAAAACTTAAAAATTG+AGG - chr8_1:75558609-75558628 None:intergenic 20.0%
!!! TTTCTTTGTTATTCTTACTA+AGG - chr8_1:75558787-75558806 None:intergenic 20.0%
!!! AGAACCCTTTTTATTTTTTT+TGG + chr8_1:75558882-75558901 Msa1214780:intron 20.0%
!! TCCACCAAAAAAAATAAAAA+GGG - chr8_1:75558889-75558908 None:intergenic 20.0%
!! TTCCACCAAAAAAAATAAAA+AGG - chr8_1:75558890-75558909 None:intergenic 20.0%
!!! TTTTATTTTTTTTGGTGGAA+TGG + chr8_1:75558890-75558909 Msa1214780:intron 20.0%
!! TTGTAATGATAAGTTGTAAT+TGG + chr8_1:75559011-75559030 Msa1214780:intron 20.0%
!! TTAAAAATGCTTGCAAAAAA+GGG - chr8_1:75561154-75561173 None:intergenic 20.0%
!! ATTAAAAATGCTTGCAAAAA+AGG - chr8_1:75561155-75561174 None:intergenic 20.0%
! ATTTGTACTTTGATATTGAC+CGG - chr8_1:75557895-75557914 None:intergenic 25.0%
!! AGAAATATAAAGAGTGGTTT+TGG + chr8_1:75558039-75558058 Msa1214780:five_prime_UTR 25.0%
!!! TTATGGTTTTAATTGATCCT+AGG + chr8_1:75558629-75558648 Msa1214780:intron 25.0%
! TTATGTTATGCTATAATCCT+AGG - chr8_1:75558649-75558668 None:intergenic 25.0%
! TATGGATGTTATATATCTTG+TGG + chr8_1:75558710-75558729 Msa1214780:intron 25.0%
!!! ACCCTTTTTATTTTTTTTGG+TGG + chr8_1:75558885-75558904 Msa1214780:intron 25.0%
!!! AGATAGTGAATTTTGTTTCT+AGG + chr8_1:75558980-75558999 Msa1214780:intron 25.0%
!!! GATAGTGAATTTTGTTTCTA+GGG + chr8_1:75558981-75559000 Msa1214780:intron 25.0%
! ATACAGATTGAATGTAACTT+GGG - chr8_1:75559837-75559856 None:intergenic 25.0%
! TTAAAAATGTAAGCGAAAGA+GGG + chr8_1:75560018-75560037 Msa1214780:CDS 25.0%
!!! TTTCGTTTTGATGTTTGAAA+GGG - chr8_1:75560294-75560313 None:intergenic 25.0%
!!! ATTTCGTTTTGATGTTTGAA+AGG - chr8_1:75560295-75560314 None:intergenic 25.0%
! TCTTTTCTATGTTCATGTAC+CGG + chr8_1:75557873-75557892 Msa1214780:five_prime_UTR 30.0%
!!! GTTTTGGTTTTTTGTGAAAG+TGG + chr8_1:75558055-75558074 Msa1214780:five_prime_UTR 30.0%
GTAACATTGTACTTATTCTG+TGG + chr8_1:75558180-75558199 Msa1214780:five_prime_UTR 30.0%
! ACAAAGCGGTTTTTATATGT+TGG - chr8_1:75558234-75558253 None:intergenic 30.0%
TTGTCATGTAACAAACAAAG+CGG - chr8_1:75558248-75558267 None:intergenic 30.0%
GGAGCAAAAAAATGCAAAAA+AGG - chr8_1:75558335-75558354 None:intergenic 30.0%
!!! TTGCATTTTTTTGCTCCTTA+AGG + chr8_1:75558338-75558357 Msa1214780:five_prime_UTR 30.0%
GAGGTATATAACAGAGAAAA+GGG - chr8_1:75558390-75558409 None:intergenic 30.0%
! AGAGGTATATAACAGAGAAA+AGG - chr8_1:75558391-75558410 None:intergenic 30.0%
GAATAATGAACAAGAAAGAG+AGG - chr8_1:75558409-75558428 None:intergenic 30.0%
TGTGATATTCAGCTTATACT+TGG + chr8_1:75558443-75558462 Msa1214780:five_prime_UTR 30.0%
CAACATGCTATAAGATAAGA+GGG - chr8_1:75558751-75558770 None:intergenic 30.0%
ACAACATGCTATAAGATAAG+AGG - chr8_1:75558752-75558771 None:intergenic 30.0%
TATAGCATGTTGTTGCAATA+AGG + chr8_1:75558759-75558778 Msa1214780:intron 30.0%
ATAGCATGTTGTTGCAATAA+GGG + chr8_1:75558760-75558779 Msa1214780:intron 30.0%
!! ATTGTGTGAATGACTTTTTC+AGG + chr8_1:75559066-75559085 Msa1214780:intron 30.0%
! TGTTTAAGGTTGTCAAGTTA+GGG - chr8_1:75559758-75559777 None:intergenic 30.0%
! ATGTTTAAGGTTGTCAAGTT+AGG - chr8_1:75559759-75559778 None:intergenic 30.0%
AGAGCATGATTCAATGTTTA+AGG - chr8_1:75559772-75559791 None:intergenic 30.0%
ATTGAATCATGCTCTATGAT+TGG + chr8_1:75559777-75559796 Msa1214780:CDS 30.0%
GATACAGATTGAATGTAACT+TGG - chr8_1:75559838-75559857 None:intergenic 30.0%
GTTACATTCAATCTGTATCA+AGG + chr8_1:75559839-75559858 Msa1214780:CDS 30.0%
!! TGTGTCTTGTTTGCTATATT+CGG + chr8_1:75559887-75559906 Msa1214780:CDS 30.0%
!!! GAATATCACTGATTTTTCTC+TGG + chr8_1:75559944-75559963 Msa1214780:CDS 30.0%
CTTAAAAATGTAAGCGAAAG+AGG + chr8_1:75560017-75560036 Msa1214780:CDS 30.0%
ATTTACACTTGTGAAGTGTA+TGG + chr8_1:75560322-75560341 Msa1214780:CDS 30.0%
TTTACACTTGTGAAGTGTAT+GGG + chr8_1:75560323-75560342 Msa1214780:CDS 30.0%
AGATTACATAGTTTCCAAGT+TGG + chr8_1:75560592-75560611 Msa1214780:CDS 30.0%
AACTTGCATCAGTAATATTC+TGG - chr8_1:75560664-75560683 None:intergenic 30.0%
GAATATTACTGATGCAAGTT+TGG + chr8_1:75560664-75560683 Msa1214780:CDS 30.0%
TTTATGGAGATGTGATATTC+TGG + chr8_1:75560937-75560956 Msa1214780:CDS 30.0%
ATTAATAATGAAGCAGCAGA+TGG + chr8_1:75561018-75561037 Msa1214780:three_prime_UTR 30.0%
!! ACATTCAGCATACTTGTTTT+TGG + chr8_1:75561043-75561062 Msa1214780:three_prime_UTR 30.0%
!!! TTGTTTTTTACAACCCCTTT+TGG + chr8_1:75561185-75561204 Msa1214780:three_prime_UTR 30.0%
! TGTTATATTCTGTTTGTTGC+AGG + chr8_1:75561301-75561320 Msa1214780:three_prime_UTR 30.0%
ATATGGTTAACCTCCTTGTA+AGG + chr8_1:75557913-75557932 Msa1214780:five_prime_UTR 35.0%
TTGAATAACCTTGCCTTACA+AGG - chr8_1:75557929-75557948 None:intergenic 35.0%
TTATTCAACAACCTCCTCAA+CGG + chr8_1:75557941-75557960 Msa1214780:five_prime_UTR 35.0%
ACGGCCCAATTTCATAAAAA+AGG + chr8_1:75557960-75557979 Msa1214780:five_prime_UTR 35.0%
!!! TGCTCCTTAAGGAGTTTTTT+TGG + chr8_1:75558349-75558368 Msa1214780:five_prime_UTR 35.0%
GATGCCAAAAAAACTCCTTA+AGG - chr8_1:75558356-75558375 None:intergenic 35.0%
GTAATGATGAATTGCATCCT+GGG + chr8_1:75559088-75559107 Msa1214780:CDS 35.0%
TAATGATGAATTGCATCCTG+GGG + chr8_1:75559089-75559108 Msa1214780:CDS 35.0%
!! GTCTCTTTGAGATATTCAGA+CGG + chr8_1:75559265-75559284 Msa1214780:CDS 35.0%
CTTGGAAAGCTATCTATTAG+AGG + chr8_1:75559519-75559538 Msa1214780:CDS 35.0%
! TGTGTCTTCTATTGGTGATA+AGG + chr8_1:75559629-75559648 Msa1214780:CDS 35.0%
! GTGTCTTCTATTGGTGATAA+GGG + chr8_1:75559630-75559649 Msa1214780:CDS 35.0%
TGCAAAAGGATGTCATATGT+TGG + chr8_1:75559665-75559684 Msa1214780:CDS 35.0%
TATTGCAATCAAACCCTTGT+TGG - chr8_1:75559724-75559743 None:intergenic 35.0%
ATGCTCTATGATTGGAAATG+AGG + chr8_1:75559785-75559804 Msa1214780:CDS 35.0%
TGCTCTATGATTGGAAATGA+GGG + chr8_1:75559786-75559805 Msa1214780:CDS 35.0%
AAGCTTTACCTTTGACAAGT+TGG - chr8_1:75559916-75559935 None:intergenic 35.0%
! GATTTTTCTCTGGCTGTTAT+TGG + chr8_1:75559954-75559973 Msa1214780:CDS 35.0%
TGTTATTGGCCATTATGGAA+AGG + chr8_1:75559968-75559987 Msa1214780:CDS 35.0%
TAACTATTACTTCCTGCCAA+GGG + chr8_1:75560087-75560106 Msa1214780:CDS 35.0%
GGGAATTAAAGACATTGATG+TGG + chr8_1:75560343-75560362 Msa1214780:CDS 35.0%
TTAACCATTCAAAACTGTCC+TGG + chr8_1:75560404-75560423 Msa1214780:CDS 35.0%
ATTCAAAACTGTCCTGGTTT+CGG + chr8_1:75560410-75560429 Msa1214780:CDS 35.0%
AGACATCTAGATCATTCAAC+AGG - chr8_1:75560712-75560731 None:intergenic 35.0%
AGTTTCTCGTGGAGAATTTA+TGG + chr8_1:75560921-75560940 Msa1214780:CDS 35.0%
GCTGAATAAGGAAATGCTAA+TGG + chr8_1:75560978-75560997 Msa1214780:three_prime_UTR 35.0%
TCATGGAACTATAGCCAAAA+GGG - chr8_1:75561202-75561221 None:intergenic 35.0%
AGTGATACAAAGTAAAACCC+AGG - chr8_1:75561277-75561296 None:intergenic 35.0%
! TGGGTTTTACTTTGTATCAC+TGG + chr8_1:75561276-75561295 Msa1214780:three_prime_UTR 35.0%
AATAACCTTGCCTTACAAGG+AGG - chr8_1:75557926-75557945 None:intergenic 40.0%
TTTATGAAATTGGGCCGTTG+AGG - chr8_1:75557958-75557977 None:intergenic 40.0%
! TGTGAAAGTGGTATTTGCTG+AGG + chr8_1:75558067-75558086 Msa1214780:five_prime_UTR 40.0%
AGTTACCACTCTGATTGACA+CGG - chr8_1:75558504-75558523 None:intergenic 40.0%
ATATATCTTGTGGCATGCGT+AGG + chr8_1:75558720-75558739 Msa1214780:intron 40.0%
GGTAATGATGAATTGCATCC+TGG + chr8_1:75559087-75559106 Msa1214780:intron 40.0%
AATGATGAATTGCATCCTGG+GGG + chr8_1:75559090-75559109 Msa1214780:CDS 40.0%
AAGAGCTCCAAAGGTAAATG+GGG - chr8_1:75559184-75559203 None:intergenic 40.0%
GAAGAGCTCCAAAGGTAAAT+GGG - chr8_1:75559185-75559204 None:intergenic 40.0%
TGAAGAGCTCCAAAGGTAAA+TGG - chr8_1:75559186-75559205 None:intergenic 40.0%
ATCTCAAAGAGACATTCGTC+CGG - chr8_1:75559258-75559277 None:intergenic 40.0%
! AAGCCACCTTTTTGATACAC+AGG - chr8_1:75559322-75559341 None:intergenic 40.0%
GTGAAAGTGATCTGAGAGAA+GGG - chr8_1:75559602-75559621 None:intergenic 40.0%
AGTGAAAGTGATCTGAGAGA+AGG - chr8_1:75559603-75559622 None:intergenic 40.0%
CTGTGGAATGTGTCTTCTAT+TGG + chr8_1:75559621-75559640 Msa1214780:CDS 40.0%
GGTCTTTGTGAGATTGCAAA+AGG + chr8_1:75559651-75559670 Msa1214780:CDS 40.0%
GGTTTGATTGCAATAGCAGA+AGG + chr8_1:75559729-75559748 Msa1214780:CDS 40.0%
ATCAAGGATTGTCCTCTTGT+TGG + chr8_1:75559855-75559874 Msa1214780:CDS 40.0%
TCGTTACTGCCTTTCCATAA+TGG - chr8_1:75559980-75559999 None:intergenic 40.0%
!! GTAACGAATCTGGTTCTAAG+TGG + chr8_1:75559993-75560012 Msa1214780:CDS 40.0%
CTAACTATTACTTCCTGCCA+AGG + chr8_1:75560086-75560105 Msa1214780:CDS 40.0%
AACTATTACTTCCTGCCAAG+GGG + chr8_1:75560088-75560107 Msa1214780:CDS 40.0%
ATACAAAGCTACATCTGCGA+AGG - chr8_1:75560172-75560191 None:intergenic 40.0%
!! TGTAGCTTTGTATCCGACAT+TGG + chr8_1:75560179-75560198 Msa1214780:CDS 40.0%
TGTAACAGATTCACCCAATG+TGG + chr8_1:75560257-75560276 Msa1214780:CDS 40.0%
GTAACAGATTCACCCAATGT+GGG + chr8_1:75560258-75560277 Msa1214780:CDS 40.0%
GGCATAGAAAATCCCACATT+GGG - chr8_1:75560273-75560292 None:intergenic 40.0%
AAAGATCGAAGAGACTTGCA+AGG - chr8_1:75560386-75560405 None:intergenic 40.0%
!!! GAAACCAGGACAGTTTTGAA+TGG - chr8_1:75560411-75560430 None:intergenic 40.0%
AGATCAACATGCTGAAGTTG+GGG - chr8_1:75560470-75560489 None:intergenic 40.0%
CAGATCAACATGCTGAAGTT+GGG - chr8_1:75560471-75560490 None:intergenic 40.0%
TCAGATCAACATGCTGAAGT+TGG - chr8_1:75560472-75560491 None:intergenic 40.0%
AATTCTCCAAAAGAGGGAGA+AGG - chr8_1:75560523-75560542 None:intergenic 40.0%
CCTCACAATTCTCCAAAAGA+GGG - chr8_1:75560529-75560548 None:intergenic 40.0%
CTCGAAATACTAAACCTCGA+TGG + chr8_1:75560635-75560654 Msa1214780:CDS 40.0%
TAATATTCTGGCATCCATCG+AGG - chr8_1:75560652-75560671 None:intergenic 40.0%
CAATGCAATTGAGTTTCTCG+TGG + chr8_1:75560910-75560929 Msa1214780:CDS 40.0%
GCTGATATGACTATCAAGTG+TGG + chr8_1:75561065-75561084 Msa1214780:three_prime_UTR 40.0%
GACTATCAAGTGTGGATTTG+CGG + chr8_1:75561073-75561092 Msa1214780:three_prime_UTR 40.0%
!! GGTTGTTTTCCAAGGAGTTT+TGG + chr8_1:75561112-75561131 Msa1214780:three_prime_UTR 40.0%
GGAAACAAACCAAAACTCCT+TGG - chr8_1:75561124-75561143 None:intergenic 40.0%
! CTTGCAAAAAAGGGCTAGTA+AGG - chr8_1:75561145-75561164 None:intergenic 40.0%
CATGGAACTATAGCCAAAAG+GGG - chr8_1:75561201-75561220 None:intergenic 40.0%
CTCATGGAACTATAGCCAAA+AGG - chr8_1:75561203-75561222 None:intergenic 40.0%
ACTCAGTAACTGCATTCTCA+TGG - chr8_1:75561219-75561238 None:intergenic 40.0%
AGAATGCAGTTACTGAGTCT+TGG + chr8_1:75561221-75561240 Msa1214780:three_prime_UTR 40.0%
AGTTACTGAGTCTTGGTTGA+TGG + chr8_1:75561228-75561247 Msa1214780:three_prime_UTR 40.0%
GTTAACCTCCTTGTAAGGCA+AGG + chr8_1:75557918-75557937 Msa1214780:five_prime_UTR 45.0%
! GAAAGTGGTATTTGCTGAGG+TGG + chr8_1:75558070-75558089 Msa1214780:five_prime_UTR 45.0%
AGAGCAGTGCACATCAACAT+TGG - chr8_1:75559136-75559155 None:intergenic 45.0%
!! CTGATTCTAGTTCGCTTCGT+AGG - chr8_1:75559159-75559178 None:intergenic 45.0%
ATCAGTGCCCCATTTACCTT+TGG + chr8_1:75559174-75559193 Msa1214780:CDS 45.0%
ATATTCAGACGGCTTCCAAG+TGG + chr8_1:75559276-75559295 Msa1214780:CDS 45.0%
CAGGTGCCTGTGTATCAAAA+AGG + chr8_1:75559313-75559332 Msa1214780:CDS 45.0%
GTGCCTGTGTATCAAAAAGG+TGG + chr8_1:75559316-75559335 Msa1214780:CDS 45.0%
GAGAGTGATGAAGATGCTGA+AGG + chr8_1:75559405-75559424 Msa1214780:CDS 45.0%
GATGAAGATGCTGAAGGTGA+TGG + chr8_1:75559411-75559430 Msa1214780:CDS 45.0%
! AAGGTGATGGCTACCTTACA+AGG + chr8_1:75559424-75559443 Msa1214780:CDS 45.0%
! TACCTTACAAGGCGTTTGGA+AGG + chr8_1:75559435-75559454 Msa1214780:CDS 45.0%
! ACCTTACAAGGCGTTTGGAA+GGG + chr8_1:75559436-75559455 Msa1214780:CDS 45.0%
ATAGCTTTCCAAGTCCACCA+CGG - chr8_1:75559512-75559531 None:intergenic 45.0%
CTCTCAGATCACTTTCACTG+TGG + chr8_1:75559604-75559623 Msa1214780:CDS 45.0%
!! TGTTGGTGATCGACGAAGAA+TGG - chr8_1:75559707-75559726 None:intergenic 45.0%
TTCTTCGTCGATCACCAACA+AGG + chr8_1:75559707-75559726 Msa1214780:CDS 45.0%
TCTTCGTCGATCACCAACAA+GGG + chr8_1:75559708-75559727 Msa1214780:CDS 45.0%
TGTCCTCTTGTTGGAGATCA+TGG + chr8_1:75559864-75559883 Msa1214780:CDS 45.0%
ACACCATGATCTCCAACAAG+AGG - chr8_1:75559870-75559889 None:intergenic 45.0%
TTCGGCTTCCAACTTGTCAA+AGG + chr8_1:75559905-75559924 Msa1214780:CDS 45.0%
CTGGCTGTTATTGGCCATTA+TGG + chr8_1:75559963-75559982 Msa1214780:CDS 45.0%
TGGAAAGGCAGTAACGAATC+TGG + chr8_1:75559983-75560002 Msa1214780:CDS 45.0%
! GAAAGAGGGTTTTGCGTCAT+GGG + chr8_1:75560032-75560051 Msa1214780:CDS 45.0%
CTTTGTATCCGACATTGGAC+TGG + chr8_1:75560184-75560203 Msa1214780:CDS 45.0%
AAATGCTGCCAGTCCAATGT+CGG - chr8_1:75560195-75560214 None:intergenic 45.0%
CTGGAGAGATCTTGTGCATT+TGG - chr8_1:75560219-75560238 None:intergenic 45.0%
GGGCATAGAAAATCCCACAT+TGG - chr8_1:75560274-75560293 None:intergenic 45.0%
CTTCAGCATGTTGATCTGAC+TGG + chr8_1:75560473-75560492 Msa1214780:CDS 45.0%
GTTGATCTGACTGGACTTTG+TGG + chr8_1:75560482-75560501 Msa1214780:CDS 45.0%
!! CCCTCTTTTGGAGAATTGTG+AGG + chr8_1:75560526-75560545 Msa1214780:CDS 45.0%
GCCTCACAATTCTCCAAAAG+AGG - chr8_1:75560530-75560549 None:intergenic 45.0%
!! CTTTTGGAGAATTGTGAGGC+TGG + chr8_1:75560530-75560549 Msa1214780:CDS 45.0%
! ACATAGTTTCCAAGTTGGCC+AGG + chr8_1:75560597-75560616 Msa1214780:CDS 45.0%
AAGTGTGCAATCACTGATGC+AGG + chr8_1:75560734-75560753 Msa1214780:CDS 45.0%
ATGATCAGCACGAGAGAGAA+CGG - chr8_1:75560765-75560784 None:intergenic 45.0%
CTCTCTATGTCTGACTGTTC+AGG + chr8_1:75560800-75560819 Msa1214780:CDS 45.0%
GTGCCTTTCCTGGTGAAATT+AGG + chr8_1:75560839-75560858 Msa1214780:CDS 45.0%
TGGCCTAATTTCACCAGGAA+AGG - chr8_1:75560845-75560864 None:intergenic 45.0%
TTTCCTGGTGAAATTAGGCC+AGG + chr8_1:75560844-75560863 Msa1214780:CDS 45.0%
ATATTCTGGCCTAATCGCGA+TGG + chr8_1:75560951-75560970 Msa1214780:exon 45.0%
!!! GTTGACTCGGTTGTTTTCCA+AGG + chr8_1:75561104-75561123 Msa1214780:three_prime_UTR 45.0%
!!! AGTTAATTTTTTTTTTTTTT+TGG + chr8_1:75558850-75558869 Msa1214780:intron 5.0%
ATGAAATTGGGCCGTTGAGG+AGG - chr8_1:75557955-75557974 None:intergenic 50.0%
! GTGGTATTTGCTGAGGTGGT+TGG + chr8_1:75558074-75558093 Msa1214780:five_prime_UTR 50.0%
! CTGTTTCTTGTGCTCATGCC+TGG + chr8_1:75558478-75558497 Msa1214780:exon 50.0%
ATGAATTGCATCCTGGGGGT+AGG + chr8_1:75559094-75559113 Msa1214780:CDS 50.0%
TGAATTGCATCCTGGGGGTA+GGG + chr8_1:75559095-75559114 Msa1214780:CDS 50.0%
GCCTACCATTGAGATCCTTC+CGG + chr8_1:75559236-75559255 Msa1214780:CDS 50.0%
TCCGGAAGGATCTCAATGGT+AGG - chr8_1:75559240-75559259 None:intergenic 50.0%
TTCGTCCGGAAGGATCTCAA+TGG - chr8_1:75559244-75559263 None:intergenic 50.0%
CAAAGAGACATTCGTCCGGA+AGG - chr8_1:75559254-75559273 None:intergenic 50.0%
GAGCTTCTCTCTTTGCCACT+TGG - chr8_1:75559294-75559313 None:intergenic 50.0%
AGTGGCAAAGAGAGAAGCTC+AGG + chr8_1:75559294-75559313 Msa1214780:CDS 50.0%
! TGGCTACCTTACAAGGCGTT+TGG + chr8_1:75559431-75559450 Msa1214780:CDS 50.0%
TCCCTTCCAAACGCCTTGTA+AGG - chr8_1:75559440-75559459 None:intergenic 50.0%
! TTACAAGGCGTTTGGAAGGG+AGG + chr8_1:75559439-75559458 Msa1214780:CDS 50.0%
GGAGGAAAGCCACTGATGTA+AGG + chr8_1:75559457-75559476 Msa1214780:CDS 50.0%
AGCAGCAAGCCTTACATCAG+TGG - chr8_1:75559469-75559488 None:intergenic 50.0%
!! AGGCTTGCTGCTATTGCTGT+TGG + chr8_1:75559477-75559496 Msa1214780:CDS 50.0%
!! GGCTTGCTGCTATTGCTGTT+GGG + chr8_1:75559478-75559497 Msa1214780:CDS 50.0%
! AGAGGAAACAACTCGACTCG+AGG + chr8_1:75559537-75559556 Msa1214780:CDS 50.0%
! CGAAAGAGGGTTTTGCGTCA+TGG + chr8_1:75560031-75560050 Msa1214780:CDS 50.0%
! AGCATCGAAGCCATGGGTAA+AGG + chr8_1:75560122-75560141 Msa1214780:CDS 50.0%
GTGTGGGAAACCTTTACCCA+TGG - chr8_1:75560135-75560154 None:intergenic 50.0%
ATCTCTCCAGAGCTTGCAGT+TGG + chr8_1:75560229-75560248 Msa1214780:CDS 50.0%
ACTCTTCCAACTGCAAGCTC+TGG - chr8_1:75560238-75560257 None:intergenic 50.0%
CTTTGTGGCATAACTGACGC+TGG + chr8_1:75560497-75560516 Msa1214780:CDS 50.0%
TTGTGAGGCTGGACTTGTCA+AGG + chr8_1:75560541-75560560 Msa1214780:CDS 50.0%
CTTGTCAAGGTGAACCTTGC+AGG + chr8_1:75560554-75560573 Msa1214780:CDS 50.0%
TCAAGTTCCAGCAACCTGCA+AGG - chr8_1:75560571-75560590 None:intergenic 50.0%
GAACAAGTGTGTGCCTTTCC+TGG + chr8_1:75560829-75560848 Msa1214780:CDS 50.0%
! GAAATTAGGCCAGGCGTTGT+CGG + chr8_1:75560853-75560872 Msa1214780:CDS 50.0%
! AAATTAGGCCAGGCGTTGTC+GGG + chr8_1:75560854-75560873 Msa1214780:CDS 50.0%
TCAGCAGTTCCATCGCGATT+AGG - chr8_1:75560963-75560982 None:intergenic 50.0%
CGCGATGGAACTGCTGAATA+AGG + chr8_1:75560966-75560985 Msa1214780:three_prime_UTR 50.0%
AGGCAATGTGCGATCTAGAG+TGG - chr8_1:75561257-75561276 None:intergenic 50.0%
ACTCTAGATCGCACATTGCC+TGG + chr8_1:75561256-75561275 Msa1214780:three_prime_UTR 50.0%
CTCTAGATCGCACATTGCCT+GGG + chr8_1:75561257-75561276 Msa1214780:three_prime_UTR 50.0%
ATAGTGTACACCCTACCCCC+AGG - chr8_1:75559108-75559127 None:intergenic 55.0%
AGCTCGCTGAAGAGCTCCAA+AGG - chr8_1:75559193-75559212 None:intergenic 55.0%
CTTCAGCGAGCTCTTGAGCA+CGG + chr8_1:75559201-75559220 Msa1214780:CDS 55.0%
TTCAGCGAGCTCTTGAGCAC+GGG + chr8_1:75559202-75559221 Msa1214780:CDS 55.0%
! GCTGCTATTGCTGTTGGGAC+TGG + chr8_1:75559483-75559502 Msa1214780:CDS 55.0%
! ACTCGAGGTGTTACAGACCG+CGG + chr8_1:75559552-75559571 Msa1214780:CDS 55.0%
GGTCTCTCAGCAGTTGCTCA+TGG + chr8_1:75559573-75559592 Msa1214780:CDS 55.0%
TGCGTCATGGGAGTTGCTCA+AGG + chr8_1:75560044-75560063 Msa1214780:CDS 55.0%
CATGGCTTCGATGCTCGCAT+CGG - chr8_1:75560117-75560136 None:intergenic 55.0%
!! GATGCGAGCATCGAAGCCAT+GGG + chr8_1:75560116-75560135 Msa1214780:CDS 55.0%
GGCACATCTGCTTCAGGTGT+GGG - chr8_1:75560151-75560170 None:intergenic 55.0%
AGGCACATCTGCTTCAGGTG+TGG - chr8_1:75560152-75560171 None:intergenic 55.0%
! TGCGAAGGCACATCTGCTTC+AGG - chr8_1:75560157-75560176 None:intergenic 55.0%
ATGCTAGCACTGCCGAAACC+AGG - chr8_1:75560425-75560444 None:intergenic 55.0%
TGGTTTCGGCAGTGCTAGCA+TGG + chr8_1:75560424-75560443 Msa1214780:CDS 55.0%
AGTGCTAGCATGGCTGTGGT+TGG + chr8_1:75560434-75560453 Msa1214780:CDS 55.0%
GTGCTAGCATGGCTGTGGTT+GGG + chr8_1:75560435-75560454 Msa1214780:CDS 55.0%
AGGTGAACCTTGCAGGTTGC+TGG + chr8_1:75560561-75560580 Msa1214780:CDS 55.0%
! TCCAAGTTGGCCAGGCTACA+TGG + chr8_1:75560605-75560624 Msa1214780:CDS 55.0%
ACCATGTAGCCTGGCCAACT+TGG - chr8_1:75560609-75560628 None:intergenic 55.0%
AAGTTGGCCAGGCTACATGG+TGG + chr8_1:75560608-75560627 Msa1214780:CDS 55.0%
GAGAGTTCCACCATGTAGCC+TGG - chr8_1:75560618-75560637 None:intergenic 55.0%
ACGCCTGGCCTAATTTCACC+AGG - chr8_1:75560850-75560869 None:intergenic 55.0%
TATTCAAGCCCGACAACGCC+TGG - chr8_1:75560865-75560884 None:intergenic 55.0%
TCAAGTGTGGATTTGCGGCG+CGG + chr8_1:75561078-75561097 Msa1214780:three_prime_UTR 55.0%
CCACTCTGATTGACACGGCC+AGG - chr8_1:75558499-75558518 None:intergenic 60.0%
CCTGGCCGTGTCAATCAGAG+TGG + chr8_1:75558496-75558515 Msa1214780:CDS 60.0%
!! ACTGGTTGCCGTGGTGGACT+TGG + chr8_1:75559501-75559520 Msa1214780:CDS 60.0%
GAGCAACTGCTGAGAGACCG+CGG - chr8_1:75559572-75559591 None:intergenic 60.0%
!! TGCTCGCATCGGTTACCCCT+TGG - chr8_1:75560106-75560125 None:intergenic 60.0%
!! CGATGCGAGCATCGAAGCCA+TGG + chr8_1:75560115-75560134 Msa1214780:CDS 60.0%
!!! CGCTGGCCTTCTCCCTCTTT+TGG + chr8_1:75560514-75560533 Msa1214780:CDS 60.0%
!! TGCGGCGCGGTTTGTTGACT+CGG + chr8_1:75561091-75561110 Msa1214780:three_prime_UTR 60.0%
!! GCTGTTGGGACTGGTTGCCG+TGG + chr8_1:75559492-75559511 Msa1214780:CDS 65.0%
!! GTTGGGACTGGTTGCCGTGG+TGG + chr8_1:75559495-75559514 Msa1214780:CDS 65.0%
!! CGCATCGGTTACCCCTTGGC+AGG - chr8_1:75560102-75560121 None:intergenic 65.0%
CGGCAGTGCTAGCATGGCTG+TGG + chr8_1:75560430-75560449 Msa1214780:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 75557844 75561405 75557844 ID=Msa1214780;Name=Msa1214780
chr8_1 mRNA 75557844 75561405 75557844 ID=Msa1214780-mRNA-1;Parent=Msa1214780;Name=Msa1214780-mRNA-1;_AED=0.00;_eAED=0.00;_QI=649|1|1|1|0|0.5|2|441|633
chr8_1 exon 75557844 75558517 75557844 ID=Msa1214780-mRNA-1:exon:25938;Parent=Msa1214780-mRNA-1
chr8_1 exon 75559088 75561405 75559088 ID=Msa1214780-mRNA-1:exon:25939;Parent=Msa1214780-mRNA-1
chr8_1 five_prime_UTR 75557844 75558492 75557844 ID=Msa1214780-mRNA-1:five_prime_utr;Parent=Msa1214780-mRNA-1
chr8_1 CDS 75558493 75558517 75558493 ID=Msa1214780-mRNA-1:cds;Parent=Msa1214780-mRNA-1
chr8_1 CDS 75559088 75560964 75559088 ID=Msa1214780-mRNA-1:cds;Parent=Msa1214780-mRNA-1
chr8_1 three_prime_UTR 75560965 75561405 75560965 ID=Msa1214780-mRNA-1:three_prime_utr;Parent=Msa1214780-mRNA-1
Gene Sequence

>Msa1214780

ATGCCTGGCCGTGTCAATCAGAGTGGTAATGATGAATTGCATCCTGGGGGTAGGGTGTACACTATTAGTTCCAATGTTGATGTGCACTGCTCTCCTACGAAGCGAACTAGAATCAGTGCCCCATTTACCTTTGGAGCTCTTCAGCGAGCTCTTGAGCACGGGCAAGATCTTAAGCCTACCATTGAGATCCTTCCGGACGAATGTCTCTTTGAGATATTCAGACGGCTTCCAAGTGGCAAAGAGAGAAGCTCAGGTGCCTGTGTATCAAAAAGGTGGCTTATGCTTATGAGCAGTATCTGCAAAGATGACATTGAATCTGATGTAGAAACAATCTCTTCAGATGAGAGTGATGAAGATGCTGAAGGTGATGGCTACCTTACAAGGCGTTTGGAAGGGAGGAAAGCCACTGATGTAAGGCTTGCTGCTATTGCTGTTGGGACTGGTTGCCGTGGTGGACTTGGAAAGCTATCTATTAGAGGAAACAACTCGACTCGAGGTGTTACAGACCGCGGTCTCTCAGCAGTTGCTCATGGTTGCCCTTCTCTCAGATCACTTTCACTGTGGAATGTGTCTTCTATTGGTGATAAGGGTCTTTGTGAGATTGCAAAAGGATGTCATATGTTGGAGACGCTCGACTTAAGCCATTCTTCGTCGATCACCAACAAGGGTTTGATTGCAATAGCAGAAGGTTGCCCTAACTTGACAACCTTAAACATTGAATCATGCTCTATGATTGGAAATGAGGGTCTGCAAGCTGTTGCAAAGTTATGTCCCAAGTTACATTCAATCTGTATCAAGGATTGTCCTCTTGTTGGAGATCATGGTGTGTCTTGTTTGCTATATTCGGCTTCCAACTTGTCAAAGGTAAAGCTTCAGATTTTGAATATCACTGATTTTTCTCTGGCTGTTATTGGCCATTATGGAAAGGCAGTAACGAATCTGGTTCTAAGTGGTCTTAAAAATGTAAGCGAAAGAGGGTTTTGCGTCATGGGAGTTGCTCAAGGTCTACAGAAATTGATGTCACTAACTATTACTTCCTGCCAAGGGGTAACCGATGCGAGCATCGAAGCCATGGGTAAAGGTTTCCCACACCTGAAGCAGATGTGCCTTCGCAGATGTAGCTTTGTATCCGACATTGGACTGGCAGCATTTGCCAAATGCACAAGATCTCTCCAGAGCTTGCAGTTGGAAGAGTGTAACAGATTCACCCAATGTGGGATTTTCTATGCCCTTTCAAACATCAAAACGAAATTGAAATCATTTACACTTGTGAAGTGTATGGGAATTAAAGACATTGATGTGGAAGTATCTATGCTTTCACCTTGCAAGTCTCTTCGATCTTTAACCATTCAAAACTGTCCTGGTTTCGGCAGTGCTAGCATGGCTGTGGTTGGGAAATTGTGTCCCCAACTTCAGCATGTTGATCTGACTGGACTTTGTGGCATAACTGACGCTGGCCTTCTCCCTCTTTTGGAGAATTGTGAGGCTGGACTTGTCAAGGTGAACCTTGCAGGTTGCTGGAACTTGACAGATTACATAGTTTCCAAGTTGGCCAGGCTACATGGTGGAACTCTCGAAATACTAAACCTCGATGGATGCCAGAATATTACTGATGCAAGTTTGGCTGCAGTTGCAGACGACTGCTTCCTGTTGAATGATCTAGATGTCTCAAAGTGTGCAATCACTGATGCAGGCATAGCCGTTCTCTCTCGTGCTGATCATCTTAGTATGCGAGTGCTCTCTATGTCTGACTGTTCAGGTATATCGAACAAGTGTGTGCCTTTCCTGGTGAAATTAGGCCAGGCGTTGTCGGGCTTGAATATTAAAAATTGCAATACAATTGACAGCAATGCAATTGAGTTTCTCGTGGAGAATTTATGGAGATGTGATATTCTGGCCTAA
Protein sequence

>Msa1214780

MPGRVNQSGNDELHPGGRVYTISSNVDVHCSPTKRTRISAPFTFGALQRALEHGQDLKPTIEILPDECLFEIFRRLPSGKERSSGACVSKRWLMLMSSICKDDIESDVETISSDESDEDAEGDGYLTRRLEGRKATDVRLAAIAVGTGCRGGLGKLSIRGNNSTRGVTDRGLSAVAHGCPSLRSLSLWNVSSIGDKGLCEIAKGCHMLETLDLSHSSSITNKGLIAIAEGCPNLTTLNIESCSMIGNEGLQAVAKLCPKLHSICIKDCPLVGDHGVSCLLYSASNLSKVKLQILNITDFSLAVIGHYGKAVTNLVLSGLKNVSERGFCVMGVAQGLQKLMSLTITSCQGVTDASIEAMGKGFPHLKQMCLRRCSFVSDIGLAAFAKCTRSLQSLQLEECNRFTQCGIFYALSNIKTKLKSFTLVKCMGIKDIDVEVSMLSPCKSLRSLTIQNCPGFGSASMAVVGKLCPQLQHVDLTGLCGITDAGLLPLLENCEAGLVKVNLAGCWNLTDYIVSKLARLHGGTLEILNLDGCQNITDASLAAVADDCFLLNDLDVSKCAITDAGIAVLSRADHLSMRVLSMSDCSGISNKCVPFLVKLGQALSGLNIKNCNTIDSNAIEFLVENLWRCDILA*