AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1300730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1300730 MtrunA17_Chr4g0063841 95.103 633 31 0 1 633 1 633 0.0 1169
Msa1300730 MtrunA17_Chr1g0149351 61.389 619 229 4 21 633 29 643 0.0 739
Msa1300730 MtrunA17_Chr3g0137771 55.814 602 258 3 34 632 44 640 0.0 642
Msa1300730 MtrunA17_Chr4g0063861 82.564 390 68 0 244 633 1 390 0.0 622
Msa1300730 MtrunA17_Chr4g0063861 24.812 399 242 9 167 536 2 371 1.52e-18 88.2
Msa1300730 MtrunA17_Chr4g0063861 23.729 295 190 7 152 414 92 383 4.99e-11 65.1
Msa1300730 MtrunA17_Chr4g0063851 81.921 177 19 3 1 171 1 170 5.84e-91 281
Msa1300730 MtrunA17_Chr2g0286991 25.990 581 367 16 64 597 11 575 8.29e-40 154
Msa1300730 MtrunA17_Chr2g0286991 26.407 462 300 10 167 625 153 577 2.65e-35 141
Msa1300730 MtrunA17_Chr2g0286991 27.873 409 283 11 219 625 127 525 4.34e-25 110
Msa1300730 MtrunA17_Chr2g0286991 29.856 278 160 5 144 418 340 585 6.72e-24 106
Msa1300730 MtrunA17_Chr2g0286991 28.435 313 210 6 167 474 281 584 5.20e-22 100
Msa1300730 MtrunA17_Chr7g0272131 28.764 445 282 11 154 586 184 605 8.23e-30 125
Msa1300730 MtrunA17_Chr7g0272131 26.496 468 274 14 182 620 135 561 2.80e-21 98.6
Msa1300730 MtrunA17_Chr7g0272131 26.263 297 186 6 165 460 399 663 3.20e-19 92.0
Msa1300730 MtrunA17_Chr4g0004901 27.021 470 299 16 129 583 179 619 8.32e-26 112
Msa1300730 MtrunA17_Chr4g0004901 26.250 480 316 15 167 619 135 603 9.45e-25 109
Msa1300730 MtrunA17_Chr4g0004901 29.204 339 203 10 166 503 366 668 2.54e-21 98.6
Msa1300730 MtrunA17_Chr1g0212071 24.706 425 277 11 62 478 18 407 1.18e-25 110
Msa1300730 MtrunA17_Chr1g0212071 25.587 426 280 11 95 508 10 410 1.23e-17 85.9
Msa1300730 MtrunA17_Chr1g0212071 23.469 294 195 6 349 614 94 385 3.37e-16 81.3
Msa1300730 MtrunA17_Chr1g0212071 31.122 196 131 4 428 622 94 286 5.94e-16 80.5
Msa1300730 MtrunA17_Chr8g0347711 27.174 368 223 10 60 403 11 357 4.21e-20 92.8
Msa1300730 MtrunA17_Chr3g0125671 31.183 186 102 2 201 386 123 282 5.94e-18 86.3
Msa1300730 MtrunA17_Chr3g0125671 32.877 146 71 3 134 255 140 282 6.87e-11 64.3
Msa1300730 MtrunA17_Chr4g0008501 22.625 579 392 21 49 597 30 582 7.38e-17 84.3
Msa1300730 MtrunA17_Chr1g0176031 30.204 245 153 5 165 399 90 326 8.16e-16 79.3
Msa1300730 MtrunA17_Chr1g0176031 21.321 333 198 8 180 506 52 326 1.05e-14 75.9
Msa1300730 MtrunA17_Chr1g0176031 29.918 244 160 8 335 577 107 340 1.49e-14 75.5
Msa1300730 MtrunA17_Chr1g0150971 24.883 426 245 15 179 583 339 710 8.82e-16 81.3
Msa1300730 MtrunA17_Chr1g0152951 24.444 360 207 10 215 570 134 432 1.13e-13 73.9
Msa1300730 MtrunA17_Chr1g0152951 37.190 121 72 3 168 286 320 438 5.22e-11 65.5
Msa1300730 MtrunA17_Chr4g0070541 26.744 258 181 7 336 590 397 649 1.14e-13 74.3
Msa1300730 MtrunA17_Chr1g0159531 33.333 153 95 3 134 285 128 274 1.61e-13 72.4
Msa1300730 MtrunA17_Chr1g0159531 28.505 214 141 3 164 365 100 313 8.11e-13 70.5
Msa1300730 MtrunA17_Chr4g0018891 25.532 329 208 13 219 545 189 482 6.32e-12 68.2
Msa1300730 MtrunA17_Chr8g0352431 23.965 459 286 17 171 597 131 558 8.79e-12 68.2
Msa1300730 MtrunA17_Chr8g0342371 23.649 444 307 15 171 597 136 564 1.03e-11 67.8
Msa1300730 MtrunA17_Chr1g0150991 23.472 409 246 15 179 583 155 500 1.62e-11 67.4
Msa1300730 MtrunA17_Chr2g0300611 24.324 259 164 5 360 590 400 654 1.74e-11 67.4
Msa1300730 MtrunA17_Chr3g0079141 22.652 543 312 21 23 544 11 466 2.44e-11 66.6
Msa1300730 MtrunA17_Chr4g0070521 24.254 268 185 7 336 590 28 290 2.84e-11 65.5
Msa1300730 MtrunA17_Chr5g0436511 25.526 380 210 18 166 506 145 490 3.71e-11 66.2
Msa1300730 MtrunA17_Chr7g0251831 28.780 205 121 9 187 374 315 511 4.71e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1300730 AT2G25490.1 51.724 638 279 7 8 633 8 628 0.0 642
Msa1300730 AT5G25350.1 52.276 637 284 7 1 632 1 622 0.0 606
Msa1300730 AT4G15475.1 28.328 586 347 19 64 597 11 575 3.69e-43 164
Msa1300730 AT4G15475.1 27.378 347 214 6 129 474 275 584 1.25e-27 118
Msa1300730 AT4G15475.1 26.575 365 232 7 167 531 281 609 1.65e-23 105
Msa1300730 AT4G15475.1 24.118 340 223 9 309 619 109 442 3.37e-16 82.8
Msa1300730 AT4G15475.1 30.769 156 103 3 143 298 443 593 6.26e-12 68.9
Msa1300730 AT5G27920.1 24.665 523 332 12 151 621 90 602 4.36e-28 120
Msa1300730 AT5G27920.1 29.268 246 168 5 167 411 364 604 1.73e-17 87.0
Msa1300730 AT5G23340.1 24.941 421 280 8 62 478 8 396 6.12e-24 105
Msa1300730 AT5G23340.1 24.684 316 207 4 296 610 85 370 3.53e-21 96.7
Msa1300730 AT5G23340.1 31.658 199 132 4 428 625 84 279 7.14e-19 89.7
Msa1300730 AT5G01720.1 26.200 500 298 17 167 610 85 569 1.81e-22 102
Msa1300730 AT5G01720.1 23.604 394 236 5 166 536 238 589 1.49e-20 96.7
Msa1300730 AT5G01720.1 20.924 368 236 9 162 505 260 596 4.48e-11 66.6
Msa1300730 AT5G01720.1 29.412 170 89 2 192 336 443 606 4.72e-11 66.2
Msa1300730 AT1G21410.2 28.351 194 113 2 193 386 104 271 6.43e-17 83.2
Msa1300730 AT1G21410.2 27.004 237 110 5 167 376 130 330 8.27e-14 73.9
Msa1300730 AT1G21410.1 28.351 194 113 2 193 386 104 271 6.43e-17 83.2
Msa1300730 AT1G21410.1 27.004 237 110 5 167 376 130 330 8.27e-14 73.9
Msa1300730 AT3G58530.1 27.132 258 154 7 145 399 102 328 1.83e-15 79.0
Msa1300730 AT3G58530.1 21.557 334 197 7 180 506 53 328 1.24e-13 73.2
Msa1300730 AT3G58530.1 25.000 244 154 5 390 608 112 351 1.09e-11 67.4
Msa1300730 AT1G47056.1 25.962 416 216 12 192 604 116 442 3.30e-15 79.3
Msa1300730 AT1G47056.1 33.918 171 102 5 124 292 260 421 1.61e-13 73.9
Msa1300730 AT1G80630.1 21.981 414 275 10 181 559 108 508 5.59e-15 78.6
Msa1300730 AT1G77000.2 28.947 228 143 5 167 376 104 330 2.77e-14 75.5
Msa1300730 AT1G77000.1 28.947 228 143 5 167 376 104 330 2.77e-14 75.5
Msa1300730 AT3G07550.1 23.098 368 239 8 52 408 6 340 2.27e-13 72.8
Msa1300730 AT3G07550.2 23.098 368 239 8 52 408 6 340 2.27e-13 72.8
Msa1300730 AT3G07550.3 24.167 360 218 8 52 408 6 313 5.01e-13 71.6
Msa1300730 AT4G30640.1 29.808 208 127 5 51 256 10 200 2.04e-12 68.9
Msa1300730 AT1G77000.3 28.324 173 98 2 193 365 104 250 1.44e-11 65.9
Msa1300730 AT1G77000.4 28.324 173 98 2 193 365 104 250 1.44e-11 65.9
Msa1300730 AT5G67250.1 23.913 368 196 10 216 570 117 413 4.31e-11 66.2
Msa1300730 AT4G07400.1 37.008 127 76 3 168 292 330 454 6.85e-11 65.9

Find 195 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGCATGATTCAATGTTTA+AGG 0.123906 8_3:-62393412 None:intergenic
CGCTGGCCTTCTCCCTCTTT+TGG 0.142205 8_3:+62394157 Msa1300730:CDS
AGTTTCTCGTGGAGAATTTA+TGG 0.177171 8_3:+62394564 Msa1300730:CDS
CTGGCTGTTATTGGCCATTA+TGG 0.192129 8_3:+62393606 Msa1300730:CDS
TGAAGAGCTCCAAAGGTAAA+TGG 0.215306 8_3:-62392826 None:intergenic
CGCGATGGAACTGCTGAATA+AGG 0.264291 8_3:+62394609 Msa1300730:three_prime_UTR
ATGTTTAAGGTTGTCAAGTT+AGG 0.283107 8_3:-62393399 None:intergenic
AGAAATATAAAGAGTGGTTT+TGG 0.296107 8_3:+62391687 Msa1300730:five_prime_UTR
TCAATATCAAAGTACAAATA+TGG 0.313413 8_3:+62391544 Msa1300730:five_prime_UTR
TGTTATATTCTGTTTGTTGC+AGG 0.317403 8_3:+62394944 Msa1300730:three_prime_UTR
TGTGTCTTGTTTGCTATATT+CGG 0.319855 8_3:+62393530 Msa1300730:CDS
TCGTTACTGCCTTTCCATAA+TGG 0.327153 8_3:-62393620 None:intergenic
GAATATTACTGATGCAAGTT+TGG 0.327338 8_3:+62394307 Msa1300730:CDS
TTTATGGAGATGTGATATTC+TGG 0.332414 8_3:+62394580 Msa1300730:CDS
CTGGAGAGATCTTGTGCATT+TGG 0.332649 8_3:-62393859 None:intergenic
CTCTCTATGTCTGACTGTTC+AGG 0.334066 8_3:+62394443 Msa1300730:CDS
AACTTGCATCAGTAATATTC+TGG 0.336340 8_3:-62394304 None:intergenic
TGTCCTCTTGTTGGAGATCA+TGG 0.346783 8_3:+62393507 Msa1300730:CDS
ATTCAAAACTGTCCTGGTTT+CGG 0.358584 8_3:+62394053 Msa1300730:CDS
CTGTTTCTTGTGCTCATGCC+TGG 0.359324 8_3:+62392126 Msa1300730:exon
CTTGTCAAGGTGAACCTTGC+AGG 0.363717 8_3:+62394197 Msa1300730:CDS
AGGTGAACCTTGCAGGTTGC+TGG 0.365852 8_3:+62394204 Msa1300730:CDS
GCTGAATAAGGAAATGCTAA+TGG 0.368749 8_3:+62394621 Msa1300730:three_prime_UTR
GTGCCTTTCCTGGTGAAATT+AGG 0.368798 8_3:+62394482 Msa1300730:CDS
ACTCTAGATCGCACATTGCC+TGG 0.374175 8_3:+62394899 Msa1300730:three_prime_UTR
TGCGAAGGCACATCTGCTTC+AGG 0.374917 8_3:-62393797 None:intergenic
ATTTACACTTGTGAAGTGTA+TGG 0.378579 8_3:+62393965 Msa1300730:CDS
GGCTTGCTGCTATTGCTGTT+GGG 0.383869 8_3:+62393121 Msa1300730:CDS
ATCAGTGCCCCATTTACCTT+TGG 0.383910 8_3:+62392817 Msa1300730:CDS
GAAATTAGGCCAGGCGTTGT+CGG 0.389515 8_3:+62394496 Msa1300730:CDS
GCTGCTATTGCTGTTGGGAC+TGG 0.389778 8_3:+62393126 Msa1300730:CDS
CTGTGGAATGTGTCTTCTAT+TGG 0.391657 8_3:+62393264 Msa1300730:CDS
GAAGAGCTCCAAAGGTAAAT+GGG 0.401450 8_3:-62392825 None:intergenic
TGTGTCTTCTATTGGTGATA+AGG 0.403727 8_3:+62393272 Msa1300730:CDS
ACCATGTAGCCTGGCCAACT+TGG 0.404543 8_3:-62394249 None:intergenic
AGAGGTATATAACAGAGAAA+AGG 0.404807 8_3:-62392036 None:intergenic
ACTGGTTGCCGTGGTGGACT+TGG 0.405676 8_3:+62393144 Msa1300730:CDS
GTGTCTTCTATTGGTGATAA+GGG 0.408571 8_3:+62393273 Msa1300730:CDS
ATTGAATCATGCTCTATGAT+TGG 0.408866 8_3:+62393420 Msa1300730:CDS
GAACAAGTGTGTGCCTTTCC+TGG 0.410656 8_3:+62394472 Msa1300730:CDS
CAGATCAACATGCTGAAGTT+GGG 0.413419 8_3:-62394111 None:intergenic
TCCCTTCCAAACGCCTTGTA+AGG 0.414436 8_3:-62393080 None:intergenic
TCAGCAGTTCCATCGCGATT+AGG 0.414562 8_3:-62394603 None:intergenic
AGGCTTGCTGCTATTGCTGT+TGG 0.416928 8_3:+62393120 Msa1300730:CDS
CTTGGAAAGCTATCTATTAG+AGG 0.419353 8_3:+62393162 Msa1300730:CDS
TGGCTACCTTACAAGGCGTT+TGG 0.422778 8_3:+62393074 Msa1300730:CDS
TCATGGAACTATAGCCAAAA+GGG 0.423795 8_3:-62394842 None:intergenic
GAGGTATATAACAGAGAAAA+GGG 0.425382 8_3:-62392035 None:intergenic
GAGCTTCTCTCTTTGCCACT+TGG 0.430900 8_3:-62392934 None:intergenic
CTCATGGAACTATAGCCAAA+AGG 0.433792 8_3:-62394843 None:intergenic
TGTTTAAGGTTGTCAAGTTA+GGG 0.434609 8_3:-62393398 None:intergenic
ATCAAGGATTGTCCTCTTGT+TGG 0.435290 8_3:+62393498 Msa1300730:CDS
GGTAATGATGAATTGCATCC+TGG 0.437251 8_3:+62392730 Msa1300730:intron
TGCGGCGCGGTTTGTTGACT+CGG 0.438068 8_3:+62394734 Msa1300730:three_prime_UTR
TATTGCAATCAAACCCTTGT+TGG 0.439489 8_3:-62393364 None:intergenic
GTTGATCTGACTGGACTTTG+CGG 0.440114 8_3:+62394125 Msa1300730:CDS
GACTATCAAGTGTGGATTTG+CGG 0.442563 8_3:+62394716 Msa1300730:three_prime_UTR
TGTTGGTGATCGACGAAGAA+TGG 0.444320 8_3:-62393347 None:intergenic
AAATTAGGCCAGGCGTTGTC+GGG 0.445281 8_3:+62394497 Msa1300730:CDS
ATTTGTACTTTGATATTGAC+CGG 0.448862 8_3:-62391540 None:intergenic
TGGAAAGGCAGTAACGAATC+TGG 0.449057 8_3:+62393626 Msa1300730:CDS
GCCTACCATTGAGATCCTTC+CGG 0.449888 8_3:+62392879 Msa1300730:CDS
TTGAATAACCTTGCCTTACA+AGG 0.450924 8_3:-62391574 None:intergenic
TCCAAGTTGGCCAGGCTACA+TGG 0.454321 8_3:+62394248 Msa1300730:CDS
AGTTACTGAGTCTTGGTTGA+TGG 0.456016 8_3:+62394871 Msa1300730:three_prime_UTR
GTTACATTCAATCTGTATCA+AGG 0.456342 8_3:+62393482 Msa1300730:CDS
AAGCTTTACCTTTGACAAGT+TGG 0.456600 8_3:-62393556 None:intergenic
TATTCAAGCCCGACAACGCC+TGG 0.460376 8_3:-62394505 None:intergenic
AGTGGCAAAGAGAGAAGCTC+AGG 0.464804 8_3:+62392937 Msa1300730:CDS
TGTGATATTCAGCTTATACT+TGG 0.466407 8_3:+62392091 Msa1300730:five_prime_UTR
CTCTAGATCGCACATTGCCT+GGG 0.467454 8_3:+62394900 Msa1300730:three_prime_UTR
AGACATCTAGATCATTCAAC+AGG 0.469504 8_3:-62394352 None:intergenic
TCAGATCAACATGCTGAAGT+TGG 0.473467 8_3:-62394112 None:intergenic
GGTCTTTGTGAGATTGCAAA+AGG 0.475591 8_3:+62393294 Msa1300730:CDS
TTAACCATTCAAAACTGTCC+TGG 0.477543 8_3:+62394047 Msa1300730:CDS
GGCATAGAAAATCCCACATT+GGG 0.478588 8_3:-62393913 None:intergenic
ATATGGTTAACCTCCTTGTA+AGG 0.479102 8_3:+62391561 Msa1300730:five_prime_UTR
ACTCTTCCAACTGCAAGCTC+TGG 0.480930 8_3:-62393878 None:intergenic
GATACAGATTGAATGTAACT+TGG 0.482768 8_3:-62393478 None:intergenic
TTCAGCGAGCTCTTGAGCAC+GGG 0.483184 8_3:+62392845 Msa1300730:CDS
CAGGTGCCTGTGTATCAAAA+AGG 0.484862 8_3:+62392956 Msa1300730:CDS
GCCTCACAATTCTCCAAAAG+AGG 0.487248 8_3:-62394170 None:intergenic
ATGCTCTATGATTGGAAATG+AGG 0.488753 8_3:+62393428 Msa1300730:CDS
TTTCCTGGTGAAATTAGGCC+AGG 0.489686 8_3:+62394487 Msa1300730:CDS
GAGAGTTCCACCATGTAGCC+TGG 0.492311 8_3:-62394258 None:intergenic
CTTTGTATCCGACATTGGAC+TGG 0.492327 8_3:+62393827 Msa1300730:CDS
ACATAGTTTCCAAGTTGGCC+AGG 0.504234 8_3:+62394240 Msa1300730:CDS
GGCACATCTGCTTCAGGTGT+GGG 0.504975 8_3:-62393791 None:intergenic
CTTCAGCGAGCTCTTGAGCA+CGG 0.505562 8_3:+62392844 Msa1300730:CDS
TGGTTTCGGCAGTGCTAGCA+TGG 0.505703 8_3:+62394067 Msa1300730:CDS
AGTGCTAGCATGGCTGTGGT+TGG 0.506787 8_3:+62394077 Msa1300730:CDS
TGCTCGCATCGGTTACCCCT+TGG 0.507539 8_3:-62393746 None:intergenic
AGATTACATAGTTTCCAAGT+TGG 0.509911 8_3:+62394235 Msa1300730:CDS
CGCATCGGTTACCCCTTGGC+AGG 0.510702 8_3:-62393742 None:intergenic
AAGTGTGCAATCACTGATGC+AGG 0.512250 8_3:+62394377 Msa1300730:CDS
GGTCTCTCAGCAGTTGCTCA+TGG 0.513210 8_3:+62393216 Msa1300730:CDS
GGAGGAAAGCCACTGATGTA+AGG 0.514553 8_3:+62393100 Msa1300730:CDS
ATCTCTCCAGAGCTTGCAGT+TGG 0.514820 8_3:+62393872 Msa1300730:CDS
TGCGTCATGGGAGTTGCTCA+AGG 0.518086 8_3:+62393687 Msa1300730:CDS
AAGGTGATGGCTACCTTACA+AGG 0.519297 8_3:+62393067 Msa1300730:CDS
GGAAACAAACCAAAACTCCT+TGG 0.519774 8_3:-62394764 None:intergenic
TGCTCTATGATTGGAAATGA+GGG 0.521873 8_3:+62393429 Msa1300730:CDS
GTGCTAGCATGGCTGTGGTT+GGG 0.523567 8_3:+62394078 Msa1300730:CDS
CCACTCTGATTGACACGGCC+AGG 0.524101 8_3:-62392144 None:intergenic
TACCTTACAAGGCGTTTGGA+AGG 0.525492 8_3:+62393078 Msa1300730:CDS
AGCATCGAAGCCATGGGTAA+AGG 0.526861 8_3:+62393765 Msa1300730:CDS
TTTACACTTGTGAAGTGTAT+GGG 0.528626 8_3:+62393966 Msa1300730:CDS
TGTAGCTTTGTATCCGACAT+TGG 0.531778 8_3:+62393822 Msa1300730:CDS
CTTCAGCATGTTGATCTGAC+TGG 0.536194 8_3:+62394116 Msa1300730:CDS
ATGAAATTGGGCCGTTGAGG+AGG 0.536524 8_3:-62391600 None:intergenic
TGCAAAAGGATGTCATATGT+TGG 0.540414 8_3:+62393308 Msa1300730:CDS
GTGGTATTTGCTGAGGTGGT+TGG 0.541778 8_3:+62391722 Msa1300730:five_prime_UTR
CTGATTCTAGTTCGCTTCGT+AGG 0.543276 8_3:-62392799 None:intergenic
GTTGGGACTGGTTGCCGTGG+TGG 0.544249 8_3:+62393138 Msa1300730:CDS
GTAACATTGTACTTATTCTG+TGG 0.544585 8_3:+62391828 Msa1300730:five_prime_UTR
GTAATGATGAATTGCATCCT+GGG 0.545999 8_3:+62392731 Msa1300730:CDS
ATCTCAAAGAGACATTCGTC+CGG 0.551142 8_3:-62392898 None:intergenic
ACTCAGTAACTGCATTCTCA+TGG 0.552061 8_3:-62394859 None:intergenic
GAAAGTGGTATTTGCTGAGG+TGG 0.552266 8_3:+62391718 Msa1300730:five_prime_UTR
TTCGGCTTCCAACTTGTCAA+AGG 0.552626 8_3:+62393548 Msa1300730:CDS
ATGCTAGCACTGCCGAAACC+AGG 0.554879 8_3:-62394065 None:intergenic
CGGCAGTGCTAGCATGGCTG+TGG 0.556009 8_3:+62394073 Msa1300730:CDS
ATTAATAATGAAGCAGCAGA+TGG 0.557908 8_3:+62394661 Msa1300730:three_prime_UTR
ATACAGATTGAATGTAACTT+GGG 0.559199 8_3:-62393477 None:intergenic
AGGCACATCTGCTTCAGGTG+TGG 0.560606 8_3:-62393792 None:intergenic
AGAATGCAGTTACTGAGTCT+TGG 0.560746 8_3:+62394864 Msa1300730:three_prime_UTR
GTGAAAGTGATCTGAGAGAA+GGG 0.561763 8_3:-62393242 None:intergenic
TGGCCTAATTTCACCAGGAA+AGG 0.564441 8_3:-62394485 None:intergenic
ATGATCAGCACGAGAGAGAA+CGG 0.564713 8_3:-62394405 None:intergenic
ACCTTACAAGGCGTTTGGAA+GGG 0.565254 8_3:+62393079 Msa1300730:CDS
AGCTCGCTGAAGAGCTCCAA+AGG 0.565614 8_3:-62392833 None:intergenic
GGTTTGATTGCAATAGCAGA+AGG 0.570491 8_3:+62393372 Msa1300730:CDS
GTTAACCTCCTTGTAAGGCA+AGG 0.573809 8_3:+62391566 Msa1300730:five_prime_UTR
TAACTATTACTTCCTGCCAA+GGG 0.575789 8_3:+62393730 Msa1300730:CDS
TTTATGAAATTGGGCCGTTG+AGG 0.576561 8_3:-62391603 None:intergenic
CTCGAAATACTAAACCTCGA+TGG 0.579655 8_3:+62394278 Msa1300730:CDS
CAAAGAGACATTCGTCCGGA+AGG 0.580269 8_3:-62392894 None:intergenic
GTGTGGGAAACCTTTACCCA+TGG 0.581206 8_3:-62393775 None:intergenic
ATGTTGTTGAATAGATGAAT+CGG 0.584105 8_3:+62391488 None:intergenic
AGCAGCAAGCCTTACATCAG+TGG 0.584954 8_3:-62393109 None:intergenic
GGGCATAGAAAATCCCACAT+TGG 0.585502 8_3:-62393914 None:intergenic
CAATGCAATTGAGTTTCTCG+TGG 0.586036 8_3:+62394553 Msa1300730:CDS
TTCTTCGTCGATCACCAACA+AGG 0.587327 8_3:+62393350 Msa1300730:CDS
AGAGCAGTGCACATCAACAT+TGG 0.587717 8_3:-62392776 None:intergenic
TGTGAAAGTGGTATTTGCTG+AGG 0.587833 8_3:+62391715 Msa1300730:five_prime_UTR
GTCTCTTTGAGATATTCAGA+CGG 0.588078 8_3:+62392908 Msa1300730:CDS
GGGAATTAAAGACATTGATG+TGG 0.591377 8_3:+62393986 Msa1300730:CDS
TTACAAGGCGTTTGGAAGGG+AGG 0.593323 8_3:+62393082 Msa1300730:CDS
CCTCACAATTCTCCAAAAGA+GGG 0.598079 8_3:-62394169 None:intergenic
ATATTCTGGCCTAATCGCGA+TGG 0.598949 8_3:+62394594 Msa1300730:exon
TGTTATTGGCCATTATGGAA+AGG 0.600886 8_3:+62393611 Msa1300730:CDS
CTCTCAGATCACTTTCACTG+TGG 0.601821 8_3:+62393247 Msa1300730:CDS
AGTGAAAGTGATCTGAGAGA+AGG 0.602549 8_3:-62393243 None:intergenic
CATGGCTTCGATGCTCGCAT+CGG 0.603091 8_3:-62393757 None:intergenic
GATGAAGATGCTGAAGGTGA+TGG 0.605262 8_3:+62393054 Msa1300730:CDS
AGGCAATGTGCGATCTAGAG+TGG 0.606144 8_3:-62394897 None:intergenic
TGTAACAGATTCACCCAATG+TGG 0.606794 8_3:+62393900 Msa1300730:CDS
AGATCAACATGCTGAAGTTG+GGG 0.609536 8_3:-62394110 None:intergenic
CTAACTATTACTTCCTGCCA+AGG 0.610927 8_3:+62393729 Msa1300730:CDS
ACACCATGATCTCCAACAAG+AGG 0.615650 8_3:-62393510 None:intergenic
TCTTCGTCGATCACCAACAA+GGG 0.621934 8_3:+62393351 Msa1300730:CDS
GAATAATGAACAAGAAAGAG+AGG 0.622380 8_3:-62392054 None:intergenic
AAATGCTGCCAGTCCAATGT+CGG 0.622406 8_3:-62393835 None:intergenic
AATTCTCCAAAAGAGGGAGA+AGG 0.622832 8_3:-62394163 None:intergenic
GATGCGAGCATCGAAGCCAT+GGG 0.623164 8_3:+62393759 Msa1300730:CDS
TTCGTCCGGAAGGATCTCAA+TGG 0.627959 8_3:-62392884 None:intergenic
ATACAAAGCTACATCTGCGA+AGG 0.636036 8_3:-62393812 None:intergenic
TCAAGTGTGGATTTGCGGCG+CGG 0.636183 8_3:+62394721 Msa1300730:three_prime_UTR
TTATTCAACAACCTCCTCAA+CGG 0.640721 8_3:+62391589 Msa1300730:five_prime_UTR
GTAACGAATCTGGTTCTAAG+TGG 0.640999 8_3:+62393636 Msa1300730:CDS
TAATGATGAATTGCATCCTG+GGG 0.643262 8_3:+62392732 Msa1300730:CDS
GCTGTTGGGACTGGTTGCCG+TGG 0.645340 8_3:+62393135 Msa1300730:CDS
AAGAGCTCCAAAGGTAAATG+GGG 0.648685 8_3:-62392824 None:intergenic
TCAAGTTCCAGCAACCTGCA+AGG 0.650087 8_3:-62394211 None:intergenic
TTGTGAGGCTGGACTTGTCA+AGG 0.655559 8_3:+62394184 Msa1300730:CDS
GCTGATATGACTATCAAGTG+TGG 0.658205 8_3:+62394708 Msa1300730:three_prime_UTR
AACTATTACTTCCTGCCAAG+GGG 0.659376 8_3:+62393731 Msa1300730:CDS
GAGCAACTGCTGAGAGACCG+CGG 0.662248 8_3:-62393212 None:intergenic
TAATATTCTGGCATCCATCG+AGG 0.665697 8_3:-62394292 None:intergenic
CATGGAACTATAGCCAAAAG+GGG 0.666185 8_3:-62394841 None:intergenic
GTAACAGATTCACCCAATGT+GGG 0.667862 8_3:+62393901 Msa1300730:CDS
TTGTCATGTAACAAACAAAG+CGG 0.667882 8_3:-62391893 None:intergenic
GAGAGTGATGAAGATGCTGA+AGG 0.668726 8_3:+62393048 Msa1300730:CDS
AATAACCTTGCCTTACAAGG+AGG 0.669394 8_3:-62391571 None:intergenic
TCCGGAAGGATCTCAATGGT+AGG 0.673037 8_3:-62392880 None:intergenic
AGAGGAAACAACTCGACTCG+AGG 0.676030 8_3:+62393180 Msa1300730:CDS
AAAGATCGAAGAGACTTGCA+AGG 0.680643 8_3:-62394026 None:intergenic
AGTGATACAAAGTAAAACCC+AGG 0.687051 8_3:-62394917 None:intergenic
ATATTCAGACGGCTTCCAAG+TGG 0.698602 8_3:+62392919 Msa1300730:CDS
AGTTACCACTCTGATTGACA+CGG 0.709344 8_3:-62392149 None:intergenic
CCTGGCCGTGTCAATCAGAG+TGG 0.712504 8_3:+62392144 Msa1300730:CDS
ACTCGAGGTGTTACAGACCG+CGG 0.719268 8_3:+62393195 Msa1300730:CDS
AAGTTGGCCAGGCTACATGG+TGG 0.728854 8_3:+62394251 Msa1300730:CDS
AATGATGAATTGCATCCTGG+GGG 0.735856 8_3:+62392733 Msa1300730:CDS
ATAGCTTTCCAAGTCCACCA+CGG 0.754154 8_3:-62393152 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTACCTTTTTTATGAAATT+GGG - chr8_3:62391615-62391634 None:intergenic 15.0%
!!! TTTTACCTTTTTTATGAAAT+TGG - chr8_3:62391616-62391635 None:intergenic 15.0%
!! ATAAAAAGAAATATAAAGAG+TGG + chr8_3:62391681-62391700 Msa1300730:five_prime_UTR 15.0%
!!! CATTAAAAAAATCGATTTTA+AGG + chr8_3:62391774-62391793 Msa1300730:five_prime_UTR 15.0%
!!! TTTTTAAGTTTTCTAGTTTA+TGG + chr8_3:62392259-62392278 Msa1300730:intron 15.0%
!! TGTTTCTTTATATCATTATA+TGG + chr8_3:62392334-62392353 Msa1300730:intron 15.0%
!! AAGAATAACAAAGAAAATAT+TGG + chr8_3:62392433-62392452 Msa1300730:intron 15.0%
!! TCAATATCAAAGTACAAATA+TGG + chr8_3:62391544-62391563 Msa1300730:five_prime_UTR 20.0%
!!! TTTATGTCTTCAATTGTTTT+GGG - chr8_3:62391666-62391685 None:intergenic 20.0%
!!! TTTTATGTCTTCAATTGTTT+TGG - chr8_3:62391667-62391686 None:intergenic 20.0%
!! GGAAATAGAAAAAAAATCTT+AGG - chr8_3:62391861-62391880 None:intergenic 20.0%
!! ACTAGAAAACTTAAAAATTG+AGG - chr8_3:62392256-62392275 None:intergenic 20.0%
!!! TTTCTTTGTTATTCTTACTA+AGG - chr8_3:62392429-62392448 None:intergenic 20.0%
!! TTGTAATGATAAGTTGTAAT+TGG + chr8_3:62392654-62392673 Msa1300730:intron 20.0%
!! TTAAAAATGCTTGCAAAAAA+GGG - chr8_3:62394797-62394816 None:intergenic 20.0%
!! ATTAAAAATGCTTGCAAAAA+AGG - chr8_3:62394798-62394817 None:intergenic 20.0%
! ATTTGTACTTTGATATTGAC+CGG - chr8_3:62391543-62391562 None:intergenic 25.0%
!! AGAAATATAAAGAGTGGTTT+TGG + chr8_3:62391687-62391706 Msa1300730:five_prime_UTR 25.0%
!!! TTATGGTTTTAATTGATCCT+AGG + chr8_3:62392276-62392295 Msa1300730:intron 25.0%
! TTATGTTATGCTATAATCCT+AGG - chr8_3:62392296-62392315 None:intergenic 25.0%
! TATGGATGTTATATATCTTG+TGG + chr8_3:62392352-62392371 Msa1300730:intron 25.0%
!!! AGAACCCTTTTTATTTTCTT+TGG + chr8_3:62392525-62392544 Msa1300730:intron 25.0%
! TCCACCAAAGAAAATAAAAA+GGG - chr8_3:62392532-62392551 None:intergenic 25.0%
! TTCCACCAAAGAAAATAAAA+AGG - chr8_3:62392533-62392552 None:intergenic 25.0%
!!! TTTTATTTTCTTTGGTGGAA+TGG + chr8_3:62392533-62392552 Msa1300730:intron 25.0%
!!! AGATAGTGAATTTTGTTTCT+AGG + chr8_3:62392623-62392642 Msa1300730:intron 25.0%
!!! GATAGTGAATTTTGTTTCTA+GGG + chr8_3:62392624-62392643 Msa1300730:intron 25.0%
! ATACAGATTGAATGTAACTT+GGG - chr8_3:62393480-62393499 None:intergenic 25.0%
! TTAAAAATGTAAGCGAAAGA+GGG + chr8_3:62393661-62393680 Msa1300730:CDS 25.0%
!!! TTTCGTTTTGATGTTTGAAA+GGG - chr8_3:62393937-62393956 None:intergenic 25.0%
!!! ATTTCGTTTTGATGTTTGAA+AGG - chr8_3:62393938-62393957 None:intergenic 25.0%
! TCTTTTCTATGTTCATGTAC+CGG + chr8_3:62391521-62391540 Msa1300730:five_prime_UTR 30.0%
!!! GTTTTGGTTTTTTGTGAAAG+TGG + chr8_3:62391703-62391722 Msa1300730:five_prime_UTR 30.0%
GTAACATTGTACTTATTCTG+TGG + chr8_3:62391828-62391847 Msa1300730:five_prime_UTR 30.0%
! ACAAAGCGGTTTTTATATGT+TGG - chr8_3:62391882-62391901 None:intergenic 30.0%
TTGTCATGTAACAAACAAAG+CGG - chr8_3:62391896-62391915 None:intergenic 30.0%
GGAGCAAAAAAATGCAAAAA+AGG - chr8_3:62391983-62392002 None:intergenic 30.0%
!!! TTGCATTTTTTTGCTCCTTA+AGG + chr8_3:62391986-62392005 Msa1300730:five_prime_UTR 30.0%
GAGGTATATAACAGAGAAAA+GGG - chr8_3:62392038-62392057 None:intergenic 30.0%
! AGAGGTATATAACAGAGAAA+AGG - chr8_3:62392039-62392058 None:intergenic 30.0%
GAATAATGAACAAGAAAGAG+AGG - chr8_3:62392057-62392076 None:intergenic 30.0%
TGTGATATTCAGCTTATACT+TGG + chr8_3:62392091-62392110 Msa1300730:five_prime_UTR 30.0%
CAACATGCTATAAGATAAGA+GGG - chr8_3:62392393-62392412 None:intergenic 30.0%
ACAACATGCTATAAGATAAG+AGG - chr8_3:62392394-62392413 None:intergenic 30.0%
TATAGCATGTTGTTGCAATA+AGG + chr8_3:62392401-62392420 Msa1300730:intron 30.0%
ATAGCATGTTGTTGCAATAA+GGG + chr8_3:62392402-62392421 Msa1300730:intron 30.0%
!!! ACCCTTTTTATTTTCTTTGG+TGG + chr8_3:62392528-62392547 Msa1300730:intron 30.0%
!! ATTGTGTGAATGACTTTTTC+AGG + chr8_3:62392709-62392728 Msa1300730:intron 30.0%
! TGTTTAAGGTTGTCAAGTTA+GGG - chr8_3:62393401-62393420 None:intergenic 30.0%
! ATGTTTAAGGTTGTCAAGTT+AGG - chr8_3:62393402-62393421 None:intergenic 30.0%
AGAGCATGATTCAATGTTTA+AGG - chr8_3:62393415-62393434 None:intergenic 30.0%
ATTGAATCATGCTCTATGAT+TGG + chr8_3:62393420-62393439 Msa1300730:CDS 30.0%
GATACAGATTGAATGTAACT+TGG - chr8_3:62393481-62393500 None:intergenic 30.0%
GTTACATTCAATCTGTATCA+AGG + chr8_3:62393482-62393501 Msa1300730:CDS 30.0%
!! TGTGTCTTGTTTGCTATATT+CGG + chr8_3:62393530-62393549 Msa1300730:CDS 30.0%
!!! GAATATCACTGATTTTTCTC+TGG + chr8_3:62393587-62393606 Msa1300730:CDS 30.0%
CTTAAAAATGTAAGCGAAAG+AGG + chr8_3:62393660-62393679 Msa1300730:CDS 30.0%
ATTTACACTTGTGAAGTGTA+TGG + chr8_3:62393965-62393984 Msa1300730:CDS 30.0%
TTTACACTTGTGAAGTGTAT+GGG + chr8_3:62393966-62393985 Msa1300730:CDS 30.0%
AGATTACATAGTTTCCAAGT+TGG + chr8_3:62394235-62394254 Msa1300730:CDS 30.0%
AACTTGCATCAGTAATATTC+TGG - chr8_3:62394307-62394326 None:intergenic 30.0%
GAATATTACTGATGCAAGTT+TGG + chr8_3:62394307-62394326 Msa1300730:CDS 30.0%
TTTATGGAGATGTGATATTC+TGG + chr8_3:62394580-62394599 Msa1300730:CDS 30.0%
ATTAATAATGAAGCAGCAGA+TGG + chr8_3:62394661-62394680 Msa1300730:three_prime_UTR 30.0%
!! ACATTCAGCATACTTGTTTT+TGG + chr8_3:62394686-62394705 Msa1300730:three_prime_UTR 30.0%
!!! TTGTTTTTTACAACCCCTTT+TGG + chr8_3:62394828-62394847 Msa1300730:three_prime_UTR 30.0%
! TGTTATATTCTGTTTGTTGC+AGG + chr8_3:62394944-62394963 Msa1300730:three_prime_UTR 30.0%
ATATGGTTAACCTCCTTGTA+AGG + chr8_3:62391561-62391580 Msa1300730:five_prime_UTR 35.0%
TTGAATAACCTTGCCTTACA+AGG - chr8_3:62391577-62391596 None:intergenic 35.0%
TTATTCAACAACCTCCTCAA+CGG + chr8_3:62391589-62391608 Msa1300730:five_prime_UTR 35.0%
ACGGCCCAATTTCATAAAAA+AGG + chr8_3:62391608-62391627 Msa1300730:five_prime_UTR 35.0%
!!! TGCTCCTTAAGGAGTTTTTT+TGG + chr8_3:62391997-62392016 Msa1300730:five_prime_UTR 35.0%
GATGCCAAAAAAACTCCTTA+AGG - chr8_3:62392004-62392023 None:intergenic 35.0%
GTAATGATGAATTGCATCCT+GGG + chr8_3:62392731-62392750 Msa1300730:CDS 35.0%
TAATGATGAATTGCATCCTG+GGG + chr8_3:62392732-62392751 Msa1300730:CDS 35.0%
!! GTCTCTTTGAGATATTCAGA+CGG + chr8_3:62392908-62392927 Msa1300730:CDS 35.0%
CTTGGAAAGCTATCTATTAG+AGG + chr8_3:62393162-62393181 Msa1300730:CDS 35.0%
! TGTGTCTTCTATTGGTGATA+AGG + chr8_3:62393272-62393291 Msa1300730:CDS 35.0%
! GTGTCTTCTATTGGTGATAA+GGG + chr8_3:62393273-62393292 Msa1300730:CDS 35.0%
TGCAAAAGGATGTCATATGT+TGG + chr8_3:62393308-62393327 Msa1300730:CDS 35.0%
TATTGCAATCAAACCCTTGT+TGG - chr8_3:62393367-62393386 None:intergenic 35.0%
ATGCTCTATGATTGGAAATG+AGG + chr8_3:62393428-62393447 Msa1300730:CDS 35.0%
TGCTCTATGATTGGAAATGA+GGG + chr8_3:62393429-62393448 Msa1300730:CDS 35.0%
AAGCTTTACCTTTGACAAGT+TGG - chr8_3:62393559-62393578 None:intergenic 35.0%
! GATTTTTCTCTGGCTGTTAT+TGG + chr8_3:62393597-62393616 Msa1300730:CDS 35.0%
TGTTATTGGCCATTATGGAA+AGG + chr8_3:62393611-62393630 Msa1300730:CDS 35.0%
TAACTATTACTTCCTGCCAA+GGG + chr8_3:62393730-62393749 Msa1300730:CDS 35.0%
GGGAATTAAAGACATTGATG+TGG + chr8_3:62393986-62394005 Msa1300730:CDS 35.0%
TTAACCATTCAAAACTGTCC+TGG + chr8_3:62394047-62394066 Msa1300730:CDS 35.0%
ATTCAAAACTGTCCTGGTTT+CGG + chr8_3:62394053-62394072 Msa1300730:CDS 35.0%
AGACATCTAGATCATTCAAC+AGG - chr8_3:62394355-62394374 None:intergenic 35.0%
AGTTTCTCGTGGAGAATTTA+TGG + chr8_3:62394564-62394583 Msa1300730:CDS 35.0%
GCTGAATAAGGAAATGCTAA+TGG + chr8_3:62394621-62394640 Msa1300730:three_prime_UTR 35.0%
TCATGGAACTATAGCCAAAA+GGG - chr8_3:62394845-62394864 None:intergenic 35.0%
AGTGATACAAAGTAAAACCC+AGG - chr8_3:62394920-62394939 None:intergenic 35.0%
! TGGGTTTTACTTTGTATCAC+TGG + chr8_3:62394919-62394938 Msa1300730:three_prime_UTR 35.0%
AATAACCTTGCCTTACAAGG+AGG - chr8_3:62391574-62391593 None:intergenic 40.0%
TTTATGAAATTGGGCCGTTG+AGG - chr8_3:62391606-62391625 None:intergenic 40.0%
! TGTGAAAGTGGTATTTGCTG+AGG + chr8_3:62391715-62391734 Msa1300730:five_prime_UTR 40.0%
AGTTACCACTCTGATTGACA+CGG - chr8_3:62392152-62392171 None:intergenic 40.0%
ATATATCTTGTGGCATGCGT+AGG + chr8_3:62392362-62392381 Msa1300730:intron 40.0%
GGTAATGATGAATTGCATCC+TGG + chr8_3:62392730-62392749 Msa1300730:intron 40.0%
AATGATGAATTGCATCCTGG+GGG + chr8_3:62392733-62392752 Msa1300730:CDS 40.0%
AAGAGCTCCAAAGGTAAATG+GGG - chr8_3:62392827-62392846 None:intergenic 40.0%
GAAGAGCTCCAAAGGTAAAT+GGG - chr8_3:62392828-62392847 None:intergenic 40.0%
TGAAGAGCTCCAAAGGTAAA+TGG - chr8_3:62392829-62392848 None:intergenic 40.0%
ATCTCAAAGAGACATTCGTC+CGG - chr8_3:62392901-62392920 None:intergenic 40.0%
! AAGCCACCTTTTTGATACAC+AGG - chr8_3:62392965-62392984 None:intergenic 40.0%
GTGAAAGTGATCTGAGAGAA+GGG - chr8_3:62393245-62393264 None:intergenic 40.0%
AGTGAAAGTGATCTGAGAGA+AGG - chr8_3:62393246-62393265 None:intergenic 40.0%
CTGTGGAATGTGTCTTCTAT+TGG + chr8_3:62393264-62393283 Msa1300730:CDS 40.0%
GGTCTTTGTGAGATTGCAAA+AGG + chr8_3:62393294-62393313 Msa1300730:CDS 40.0%
GGTTTGATTGCAATAGCAGA+AGG + chr8_3:62393372-62393391 Msa1300730:CDS 40.0%
ATCAAGGATTGTCCTCTTGT+TGG + chr8_3:62393498-62393517 Msa1300730:CDS 40.0%
TCGTTACTGCCTTTCCATAA+TGG - chr8_3:62393623-62393642 None:intergenic 40.0%
!! GTAACGAATCTGGTTCTAAG+TGG + chr8_3:62393636-62393655 Msa1300730:CDS 40.0%
CTAACTATTACTTCCTGCCA+AGG + chr8_3:62393729-62393748 Msa1300730:CDS 40.0%
AACTATTACTTCCTGCCAAG+GGG + chr8_3:62393731-62393750 Msa1300730:CDS 40.0%
ATACAAAGCTACATCTGCGA+AGG - chr8_3:62393815-62393834 None:intergenic 40.0%
!! TGTAGCTTTGTATCCGACAT+TGG + chr8_3:62393822-62393841 Msa1300730:CDS 40.0%
TGTAACAGATTCACCCAATG+TGG + chr8_3:62393900-62393919 Msa1300730:CDS 40.0%
GTAACAGATTCACCCAATGT+GGG + chr8_3:62393901-62393920 Msa1300730:CDS 40.0%
GGCATAGAAAATCCCACATT+GGG - chr8_3:62393916-62393935 None:intergenic 40.0%
AAAGATCGAAGAGACTTGCA+AGG - chr8_3:62394029-62394048 None:intergenic 40.0%
!!! GAAACCAGGACAGTTTTGAA+TGG - chr8_3:62394054-62394073 None:intergenic 40.0%
AGATCAACATGCTGAAGTTG+GGG - chr8_3:62394113-62394132 None:intergenic 40.0%
CAGATCAACATGCTGAAGTT+GGG - chr8_3:62394114-62394133 None:intergenic 40.0%
TCAGATCAACATGCTGAAGT+TGG - chr8_3:62394115-62394134 None:intergenic 40.0%
AATTCTCCAAAAGAGGGAGA+AGG - chr8_3:62394166-62394185 None:intergenic 40.0%
CCTCACAATTCTCCAAAAGA+GGG - chr8_3:62394172-62394191 None:intergenic 40.0%
CTCGAAATACTAAACCTCGA+TGG + chr8_3:62394278-62394297 Msa1300730:CDS 40.0%
TAATATTCTGGCATCCATCG+AGG - chr8_3:62394295-62394314 None:intergenic 40.0%
CAATGCAATTGAGTTTCTCG+TGG + chr8_3:62394553-62394572 Msa1300730:CDS 40.0%
GCTGATATGACTATCAAGTG+TGG + chr8_3:62394708-62394727 Msa1300730:three_prime_UTR 40.0%
GACTATCAAGTGTGGATTTG+CGG + chr8_3:62394716-62394735 Msa1300730:three_prime_UTR 40.0%
!! GGTTGTTTTCCAAGGAGTTT+TGG + chr8_3:62394755-62394774 Msa1300730:three_prime_UTR 40.0%
GGAAACAAACCAAAACTCCT+TGG - chr8_3:62394767-62394786 None:intergenic 40.0%
! CTTGCAAAAAAGGGCTAGTA+AGG - chr8_3:62394788-62394807 None:intergenic 40.0%
CATGGAACTATAGCCAAAAG+GGG - chr8_3:62394844-62394863 None:intergenic 40.0%
CTCATGGAACTATAGCCAAA+AGG - chr8_3:62394846-62394865 None:intergenic 40.0%
ACTCAGTAACTGCATTCTCA+TGG - chr8_3:62394862-62394881 None:intergenic 40.0%
AGAATGCAGTTACTGAGTCT+TGG + chr8_3:62394864-62394883 Msa1300730:three_prime_UTR 40.0%
AGTTACTGAGTCTTGGTTGA+TGG + chr8_3:62394871-62394890 Msa1300730:three_prime_UTR 40.0%
GTTAACCTCCTTGTAAGGCA+AGG + chr8_3:62391566-62391585 Msa1300730:five_prime_UTR 45.0%
! GAAAGTGGTATTTGCTGAGG+TGG + chr8_3:62391718-62391737 Msa1300730:five_prime_UTR 45.0%
AGAGCAGTGCACATCAACAT+TGG - chr8_3:62392779-62392798 None:intergenic 45.0%
!! CTGATTCTAGTTCGCTTCGT+AGG - chr8_3:62392802-62392821 None:intergenic 45.0%
ATCAGTGCCCCATTTACCTT+TGG + chr8_3:62392817-62392836 Msa1300730:CDS 45.0%
ATATTCAGACGGCTTCCAAG+TGG + chr8_3:62392919-62392938 Msa1300730:CDS 45.0%
CAGGTGCCTGTGTATCAAAA+AGG + chr8_3:62392956-62392975 Msa1300730:CDS 45.0%
GTGCCTGTGTATCAAAAAGG+TGG + chr8_3:62392959-62392978 Msa1300730:CDS 45.0%
GAGAGTGATGAAGATGCTGA+AGG + chr8_3:62393048-62393067 Msa1300730:CDS 45.0%
GATGAAGATGCTGAAGGTGA+TGG + chr8_3:62393054-62393073 Msa1300730:CDS 45.0%
! AAGGTGATGGCTACCTTACA+AGG + chr8_3:62393067-62393086 Msa1300730:CDS 45.0%
! TACCTTACAAGGCGTTTGGA+AGG + chr8_3:62393078-62393097 Msa1300730:CDS 45.0%
! ACCTTACAAGGCGTTTGGAA+GGG + chr8_3:62393079-62393098 Msa1300730:CDS 45.0%
ATAGCTTTCCAAGTCCACCA+CGG - chr8_3:62393155-62393174 None:intergenic 45.0%
CTCTCAGATCACTTTCACTG+TGG + chr8_3:62393247-62393266 Msa1300730:CDS 45.0%
!! TGTTGGTGATCGACGAAGAA+TGG - chr8_3:62393350-62393369 None:intergenic 45.0%
TTCTTCGTCGATCACCAACA+AGG + chr8_3:62393350-62393369 Msa1300730:CDS 45.0%
TCTTCGTCGATCACCAACAA+GGG + chr8_3:62393351-62393370 Msa1300730:CDS 45.0%
TGTCCTCTTGTTGGAGATCA+TGG + chr8_3:62393507-62393526 Msa1300730:CDS 45.0%
ACACCATGATCTCCAACAAG+AGG - chr8_3:62393513-62393532 None:intergenic 45.0%
TTCGGCTTCCAACTTGTCAA+AGG + chr8_3:62393548-62393567 Msa1300730:CDS 45.0%
CTGGCTGTTATTGGCCATTA+TGG + chr8_3:62393606-62393625 Msa1300730:CDS 45.0%
TGGAAAGGCAGTAACGAATC+TGG + chr8_3:62393626-62393645 Msa1300730:CDS 45.0%
! GAAAGAGGGTTTTGCGTCAT+GGG + chr8_3:62393675-62393694 Msa1300730:CDS 45.0%
CTTTGTATCCGACATTGGAC+TGG + chr8_3:62393827-62393846 Msa1300730:CDS 45.0%
AAATGCTGCCAGTCCAATGT+CGG - chr8_3:62393838-62393857 None:intergenic 45.0%
CTGGAGAGATCTTGTGCATT+TGG - chr8_3:62393862-62393881 None:intergenic 45.0%
GGGCATAGAAAATCCCACAT+TGG - chr8_3:62393917-62393936 None:intergenic 45.0%
CTTCAGCATGTTGATCTGAC+TGG + chr8_3:62394116-62394135 Msa1300730:CDS 45.0%
GTTGATCTGACTGGACTTTG+CGG + chr8_3:62394125-62394144 Msa1300730:CDS 45.0%
!! CCCTCTTTTGGAGAATTGTG+AGG + chr8_3:62394169-62394188 Msa1300730:CDS 45.0%
GCCTCACAATTCTCCAAAAG+AGG - chr8_3:62394173-62394192 None:intergenic 45.0%
!! CTTTTGGAGAATTGTGAGGC+TGG + chr8_3:62394173-62394192 Msa1300730:CDS 45.0%
! ACATAGTTTCCAAGTTGGCC+AGG + chr8_3:62394240-62394259 Msa1300730:CDS 45.0%
AAGTGTGCAATCACTGATGC+AGG + chr8_3:62394377-62394396 Msa1300730:CDS 45.0%
ATGATCAGCACGAGAGAGAA+CGG - chr8_3:62394408-62394427 None:intergenic 45.0%
CTCTCTATGTCTGACTGTTC+AGG + chr8_3:62394443-62394462 Msa1300730:CDS 45.0%
GTGCCTTTCCTGGTGAAATT+AGG + chr8_3:62394482-62394501 Msa1300730:CDS 45.0%
TGGCCTAATTTCACCAGGAA+AGG - chr8_3:62394488-62394507 None:intergenic 45.0%
TTTCCTGGTGAAATTAGGCC+AGG + chr8_3:62394487-62394506 Msa1300730:CDS 45.0%
ATATTCTGGCCTAATCGCGA+TGG + chr8_3:62394594-62394613 Msa1300730:exon 45.0%
!!! GTTGACTCGGTTGTTTTCCA+AGG + chr8_3:62394747-62394766 Msa1300730:three_prime_UTR 45.0%
!!! GTTAATTTTTTTTTTTTTTT+TGG + chr8_3:62392493-62392512 Msa1300730:intron 5.0%
ATGAAATTGGGCCGTTGAGG+AGG - chr8_3:62391603-62391622 None:intergenic 50.0%
! GTGGTATTTGCTGAGGTGGT+TGG + chr8_3:62391722-62391741 Msa1300730:five_prime_UTR 50.0%
! CTGTTTCTTGTGCTCATGCC+TGG + chr8_3:62392126-62392145 Msa1300730:exon 50.0%
ATGAATTGCATCCTGGGGGT+AGG + chr8_3:62392737-62392756 Msa1300730:CDS 50.0%
TGAATTGCATCCTGGGGGTA+GGG + chr8_3:62392738-62392757 Msa1300730:CDS 50.0%
GCCTACCATTGAGATCCTTC+CGG + chr8_3:62392879-62392898 Msa1300730:CDS 50.0%
TCCGGAAGGATCTCAATGGT+AGG - chr8_3:62392883-62392902 None:intergenic 50.0%
TTCGTCCGGAAGGATCTCAA+TGG - chr8_3:62392887-62392906 None:intergenic 50.0%
CAAAGAGACATTCGTCCGGA+AGG - chr8_3:62392897-62392916 None:intergenic 50.0%
GAGCTTCTCTCTTTGCCACT+TGG - chr8_3:62392937-62392956 None:intergenic 50.0%
AGTGGCAAAGAGAGAAGCTC+AGG + chr8_3:62392937-62392956 Msa1300730:CDS 50.0%
! TGGCTACCTTACAAGGCGTT+TGG + chr8_3:62393074-62393093 Msa1300730:CDS 50.0%
TCCCTTCCAAACGCCTTGTA+AGG - chr8_3:62393083-62393102 None:intergenic 50.0%
! TTACAAGGCGTTTGGAAGGG+AGG + chr8_3:62393082-62393101 Msa1300730:CDS 50.0%
GGAGGAAAGCCACTGATGTA+AGG + chr8_3:62393100-62393119 Msa1300730:CDS 50.0%
AGCAGCAAGCCTTACATCAG+TGG - chr8_3:62393112-62393131 None:intergenic 50.0%
!! AGGCTTGCTGCTATTGCTGT+TGG + chr8_3:62393120-62393139 Msa1300730:CDS 50.0%
!! GGCTTGCTGCTATTGCTGTT+GGG + chr8_3:62393121-62393140 Msa1300730:CDS 50.0%
! AGAGGAAACAACTCGACTCG+AGG + chr8_3:62393180-62393199 Msa1300730:CDS 50.0%
! CGAAAGAGGGTTTTGCGTCA+TGG + chr8_3:62393674-62393693 Msa1300730:CDS 50.0%
! AGCATCGAAGCCATGGGTAA+AGG + chr8_3:62393765-62393784 Msa1300730:CDS 50.0%
GTGTGGGAAACCTTTACCCA+TGG - chr8_3:62393778-62393797 None:intergenic 50.0%
ATCTCTCCAGAGCTTGCAGT+TGG + chr8_3:62393872-62393891 Msa1300730:CDS 50.0%
ACTCTTCCAACTGCAAGCTC+TGG - chr8_3:62393881-62393900 None:intergenic 50.0%
TTGTGAGGCTGGACTTGTCA+AGG + chr8_3:62394184-62394203 Msa1300730:CDS 50.0%
CTTGTCAAGGTGAACCTTGC+AGG + chr8_3:62394197-62394216 Msa1300730:CDS 50.0%
TCAAGTTCCAGCAACCTGCA+AGG - chr8_3:62394214-62394233 None:intergenic 50.0%
GAACAAGTGTGTGCCTTTCC+TGG + chr8_3:62394472-62394491 Msa1300730:CDS 50.0%
! GAAATTAGGCCAGGCGTTGT+CGG + chr8_3:62394496-62394515 Msa1300730:CDS 50.0%
! AAATTAGGCCAGGCGTTGTC+GGG + chr8_3:62394497-62394516 Msa1300730:CDS 50.0%
TCAGCAGTTCCATCGCGATT+AGG - chr8_3:62394606-62394625 None:intergenic 50.0%
CGCGATGGAACTGCTGAATA+AGG + chr8_3:62394609-62394628 Msa1300730:three_prime_UTR 50.0%
AGGCAATGTGCGATCTAGAG+TGG - chr8_3:62394900-62394919 None:intergenic 50.0%
ACTCTAGATCGCACATTGCC+TGG + chr8_3:62394899-62394918 Msa1300730:three_prime_UTR 50.0%
CTCTAGATCGCACATTGCCT+GGG + chr8_3:62394900-62394919 Msa1300730:three_prime_UTR 50.0%
ATAGTGTACACCCTACCCCC+AGG - chr8_3:62392751-62392770 None:intergenic 55.0%
AGCTCGCTGAAGAGCTCCAA+AGG - chr8_3:62392836-62392855 None:intergenic 55.0%
CTTCAGCGAGCTCTTGAGCA+CGG + chr8_3:62392844-62392863 Msa1300730:CDS 55.0%
TTCAGCGAGCTCTTGAGCAC+GGG + chr8_3:62392845-62392864 Msa1300730:CDS 55.0%
! GCTGCTATTGCTGTTGGGAC+TGG + chr8_3:62393126-62393145 Msa1300730:CDS 55.0%
! ACTCGAGGTGTTACAGACCG+CGG + chr8_3:62393195-62393214 Msa1300730:CDS 55.0%
GGTCTCTCAGCAGTTGCTCA+TGG + chr8_3:62393216-62393235 Msa1300730:CDS 55.0%
TGCGTCATGGGAGTTGCTCA+AGG + chr8_3:62393687-62393706 Msa1300730:CDS 55.0%
CATGGCTTCGATGCTCGCAT+CGG - chr8_3:62393760-62393779 None:intergenic 55.0%
!! GATGCGAGCATCGAAGCCAT+GGG + chr8_3:62393759-62393778 Msa1300730:CDS 55.0%
GGCACATCTGCTTCAGGTGT+GGG - chr8_3:62393794-62393813 None:intergenic 55.0%
AGGCACATCTGCTTCAGGTG+TGG - chr8_3:62393795-62393814 None:intergenic 55.0%
! TGCGAAGGCACATCTGCTTC+AGG - chr8_3:62393800-62393819 None:intergenic 55.0%
ATGCTAGCACTGCCGAAACC+AGG - chr8_3:62394068-62394087 None:intergenic 55.0%
TGGTTTCGGCAGTGCTAGCA+TGG + chr8_3:62394067-62394086 Msa1300730:CDS 55.0%
AGTGCTAGCATGGCTGTGGT+TGG + chr8_3:62394077-62394096 Msa1300730:CDS 55.0%
GTGCTAGCATGGCTGTGGTT+GGG + chr8_3:62394078-62394097 Msa1300730:CDS 55.0%
CTTTGCGGCATAACTGACGC+TGG + chr8_3:62394140-62394159 Msa1300730:CDS 55.0%
AGGTGAACCTTGCAGGTTGC+TGG + chr8_3:62394204-62394223 Msa1300730:CDS 55.0%
! TCCAAGTTGGCCAGGCTACA+TGG + chr8_3:62394248-62394267 Msa1300730:CDS 55.0%
ACCATGTAGCCTGGCCAACT+TGG - chr8_3:62394252-62394271 None:intergenic 55.0%
AAGTTGGCCAGGCTACATGG+TGG + chr8_3:62394251-62394270 Msa1300730:CDS 55.0%
GAGAGTTCCACCATGTAGCC+TGG - chr8_3:62394261-62394280 None:intergenic 55.0%
ACGCCTGGCCTAATTTCACC+AGG - chr8_3:62394493-62394512 None:intergenic 55.0%
TATTCAAGCCCGACAACGCC+TGG - chr8_3:62394508-62394527 None:intergenic 55.0%
TCAAGTGTGGATTTGCGGCG+CGG + chr8_3:62394721-62394740 Msa1300730:three_prime_UTR 55.0%
CCACTCTGATTGACACGGCC+AGG - chr8_3:62392147-62392166 None:intergenic 60.0%
CCTGGCCGTGTCAATCAGAG+TGG + chr8_3:62392144-62392163 Msa1300730:CDS 60.0%
!! ACTGGTTGCCGTGGTGGACT+TGG + chr8_3:62393144-62393163 Msa1300730:CDS 60.0%
GAGCAACTGCTGAGAGACCG+CGG - chr8_3:62393215-62393234 None:intergenic 60.0%
!! TGCTCGCATCGGTTACCCCT+TGG - chr8_3:62393749-62393768 None:intergenic 60.0%
!! CGATGCGAGCATCGAAGCCA+TGG + chr8_3:62393758-62393777 Msa1300730:CDS 60.0%
!!! CGCTGGCCTTCTCCCTCTTT+TGG + chr8_3:62394157-62394176 Msa1300730:CDS 60.0%
!! TGCGGCGCGGTTTGTTGACT+CGG + chr8_3:62394734-62394753 Msa1300730:three_prime_UTR 60.0%
!! GCTGTTGGGACTGGTTGCCG+TGG + chr8_3:62393135-62393154 Msa1300730:CDS 65.0%
!! GTTGGGACTGGTTGCCGTGG+TGG + chr8_3:62393138-62393157 Msa1300730:CDS 65.0%
!! CGCATCGGTTACCCCTTGGC+AGG - chr8_3:62393745-62393764 None:intergenic 65.0%
CGGCAGTGCTAGCATGGCTG+TGG + chr8_3:62394073-62394092 Msa1300730:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 62391492 62395047 62391492 ID=Msa1300730;Name=Msa1300730
chr8_3 mRNA 62391492 62395047 62391492 ID=Msa1300730-mRNA-1;Parent=Msa1300730;Name=Msa1300730-mRNA-1;_AED=0.22;_eAED=0.22;_QI=649|1|1|1|1|1|2|440|633
chr8_3 exon 62391492 62392165 62391492 ID=Msa1300730-mRNA-1:exon:18557;Parent=Msa1300730-mRNA-1
chr8_3 exon 62392731 62395047 62392731 ID=Msa1300730-mRNA-1:exon:18558;Parent=Msa1300730-mRNA-1
chr8_3 five_prime_UTR 62391492 62392140 62391492 ID=Msa1300730-mRNA-1:five_prime_utr;Parent=Msa1300730-mRNA-1
chr8_3 CDS 62392141 62392165 62392141 ID=Msa1300730-mRNA-1:cds;Parent=Msa1300730-mRNA-1
chr8_3 CDS 62392731 62394607 62392731 ID=Msa1300730-mRNA-1:cds;Parent=Msa1300730-mRNA-1
chr8_3 three_prime_UTR 62394608 62395047 62394608 ID=Msa1300730-mRNA-1:three_prime_utr;Parent=Msa1300730-mRNA-1
Gene Sequence

>Msa1300730

ATGCCTGGCCGTGTCAATCAGAGTGGTAATGATGAATTGCATCCTGGGGGTAGGGTGTACACTATTAGTTCCAATGTTGATGTGCACTGCTCTCCTACGAAGCGAACTAGAATCAGTGCCCCATTTACCTTTGGAGCTCTTCAGCGAGCTCTTGAGCACGGGCAAGATCTTAAGCCTACCATTGAGATCCTTCCGGACGAATGTCTCTTTGAGATATTCAGACGGCTTCCAAGTGGCAAAGAGAGAAGCTCAGGTGCCTGTGTATCAAAAAGGTGGCTTATGCTTATGAGCAGTATCTGCAAAGATGACATTGAATCTGATGTAGAAACAATCTCTTCAGATGAGAGTGATGAAGATGCTGAAGGTGATGGCTACCTTACAAGGCGTTTGGAAGGGAGGAAAGCCACTGATGTAAGGCTTGCTGCTATTGCTGTTGGGACTGGTTGCCGTGGTGGACTTGGAAAGCTATCTATTAGAGGAAACAACTCGACTCGAGGTGTTACAGACCGCGGTCTCTCAGCAGTTGCTCATGGTTGCCCTTCTCTCAGATCACTTTCACTGTGGAATGTGTCTTCTATTGGTGATAAGGGTCTTTGTGAGATTGCAAAAGGATGTCATATGTTGGAGACGCTCGACTTAAGCCATTCTTCGTCGATCACCAACAAGGGTTTGATTGCAATAGCAGAAGGTTGCCCTAACTTGACAACCTTAAACATTGAATCATGCTCTATGATTGGAAATGAGGGTCTGCAAGCTGTTGCAAAGTTATGTCCCAAGTTACATTCAATCTGTATCAAGGATTGTCCTCTTGTTGGAGATCATGGTGTGTCTTGTTTGCTATATTCGGCTTCCAACTTGTCAAAGGTAAAGCTTCAGATTTTGAATATCACTGATTTTTCTCTGGCTGTTATTGGCCATTATGGAAAGGCAGTAACGAATCTGGTTCTAAGTGGTCTTAAAAATGTAAGCGAAAGAGGGTTTTGCGTCATGGGAGTTGCTCAAGGTCTACAGAAATTGATGTCACTAACTATTACTTCCTGCCAAGGGGTAACCGATGCGAGCATCGAAGCCATGGGTAAAGGTTTCCCACACCTGAAGCAGATGTGCCTTCGCAGATGTAGCTTTGTATCCGACATTGGACTGGCAGCATTTGCCAAATGCACAAGATCTCTCCAGAGCTTGCAGTTGGAAGAGTGTAACAGATTCACCCAATGTGGGATTTTCTATGCCCTTTCAAACATCAAAACGAAATTGAAATCATTTACACTTGTGAAGTGTATGGGAATTAAAGACATTGATGTGGAAGTATCTATGCTTTCACCTTGCAAGTCTCTTCGATCTTTAACCATTCAAAACTGTCCTGGTTTCGGCAGTGCTAGCATGGCTGTGGTTGGGAAATTGTGTCCCCAACTTCAGCATGTTGATCTGACTGGACTTTGCGGCATAACTGACGCTGGCCTTCTCCCTCTTTTGGAGAATTGTGAGGCTGGACTTGTCAAGGTGAACCTTGCAGGTTGCTGGAACTTGACAGATTACATAGTTTCCAAGTTGGCCAGGCTACATGGTGGAACTCTCGAAATACTAAACCTCGATGGATGCCAGAATATTACTGATGCAAGTTTGGCTGCAGTTGCAGACGACTGCTTCCTGTTGAATGATCTAGATGTCTCAAAGTGTGCAATCACTGATGCAGGCATAGCCGTTCTCTCTCGTGCTGATCATCTTAGTATGCGAGTGCTCTCTATGTCTGACTGTTCAGGTATATCGAACAAGTGTGTGCCTTTCCTGGTGAAATTAGGCCAGGCGTTGTCGGGCTTGAATATTAAAAATTGCAATACAATTGACAGCAATGCAATTGAGTTTCTCGTGGAGAATTTATGGAGATGTGATATTCTGGCCTAA
Protein sequence

>Msa1300730

MPGRVNQSGNDELHPGGRVYTISSNVDVHCSPTKRTRISAPFTFGALQRALEHGQDLKPTIEILPDECLFEIFRRLPSGKERSSGACVSKRWLMLMSSICKDDIESDVETISSDESDEDAEGDGYLTRRLEGRKATDVRLAAIAVGTGCRGGLGKLSIRGNNSTRGVTDRGLSAVAHGCPSLRSLSLWNVSSIGDKGLCEIAKGCHMLETLDLSHSSSITNKGLIAIAEGCPNLTTLNIESCSMIGNEGLQAVAKLCPKLHSICIKDCPLVGDHGVSCLLYSASNLSKVKLQILNITDFSLAVIGHYGKAVTNLVLSGLKNVSERGFCVMGVAQGLQKLMSLTITSCQGVTDASIEAMGKGFPHLKQMCLRRCSFVSDIGLAAFAKCTRSLQSLQLEECNRFTQCGIFYALSNIKTKLKSFTLVKCMGIKDIDVEVSMLSPCKSLRSLTIQNCPGFGSASMAVVGKLCPQLQHVDLTGLCGITDAGLLPLLENCEAGLVKVNLAGCWNLTDYIVSKLARLHGGTLEILNLDGCQNITDASLAAVADDCFLLNDLDVSKCAITDAGIAVLSRADHLSMRVLSMSDCSGISNKCVPFLVKLGQALSGLNIKNCNTIDSNAIEFLVENLWRCDILA*