AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1267970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1267970 MtrunA17_Chr8g0366731 88.312 77 8 1 70 146 334 409 6.14e-36 127
Msa1267970 MtrunA17_Chr8g0366741 90.164 61 6 0 86 146 341 401 2.10e-30 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1267970 AT4G17890.1 65.217 69 23 1 79 146 345 413 3.78e-22 91.3
Msa1267970 AT5G46750.1 65.217 69 23 1 79 146 334 402 2.90e-21 88.6
Msa1267970 AT2G35210.1 73.333 60 15 1 79 137 334 393 6.27e-18 79.3

Find 34 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTATTCGTTATAATTTAT+CGG 0.223385 8_3:+398813 Msa1267970:CDS
TTAAGGTTGCTAATATGTTT+TGG 0.302340 8_3:+399672 Msa1267970:three_prime_UTR
CTCCTTGATGACAGATCTTC+AGG 0.326640 8_3:+399448 Msa1267970:CDS
TATCAATAGAATATCTTTCC+AGG 0.348903 8_3:+399231 Msa1267970:CDS
AAGAACATTGCTGGAGAGAC+TGG 0.349107 8_3:+399401 Msa1267970:CDS
TCTCTCCAGCAATGTTCTTA+AGG 0.357125 8_3:-399397 None:intergenic
TCTTCCCTTAAGAACATTGC+TGG 0.364267 8_3:+399392 Msa1267970:CDS
CTCTCCAGCAATGTTCTTAA+GGG 0.388884 8_3:-399396 None:intergenic
TATTACATTGAAAACATAAA+CGG 0.392148 8_3:-399537 None:intergenic
AAAAGCATGTTCAAATGCAT+TGG 0.425101 8_3:-398769 None:intergenic
TTCAAGTTTGTCATTTAGAC+TGG 0.444089 8_3:+399617 Msa1267970:three_prime_UTR
TCGTTATAATTTATCGGCTA+GGG 0.454589 8_3:+398819 Msa1267970:CDS
TTCGTTATAATTTATCGGCT+AGG 0.460112 8_3:+398818 Msa1267970:CDS
AAAGCATGTTCAAATGCATT+GGG 0.467050 8_3:-398768 None:intergenic
ATATAGATATGTTGAGGGAT+TGG 0.479007 8_3:+398958 Msa1267970:CDS
GGAAAGATATTCTATTGATA+AGG 0.495577 8_3:-399228 None:intergenic
GGGATTGGTAAGCTATGTTC+CGG 0.507957 8_3:+398973 Msa1267970:CDS
GTCAATACTGGTTTGTCGCA+AGG 0.513177 8_3:+399718 Msa1267970:three_prime_UTR
TATAGATATAGATATGTTGA+GGG 0.517732 8_3:+398953 Msa1267970:CDS
TCTGTATTTAAGAGAACCTC+CGG 0.523748 8_3:+399495 Msa1267970:three_prime_UTR
GAGTTTCCACTCGTAAACAT+TGG 0.545929 8_3:-398673 None:intergenic
AAAAGATTGTGAACAGAACA+AGG 0.549809 8_3:-399765 None:intergenic
CTATAATATCTGAAATTCCA+TGG 0.551681 8_3:-398935 None:intergenic
TTATAGATATAGATATGTTG+AGG 0.573082 8_3:+398952 Msa1267970:CDS
GTGTAAAGCAAATTAAGCCA+TGG 0.576820 8_3:+398918 Msa1267970:intron
AGAACATTGCTGGAGAGACT+GGG 0.589784 8_3:+399402 Msa1267970:CDS
TGTAACCCAATGTTTACGAG+TGG 0.597198 8_3:+398667 None:intergenic
TGTCATCAAGGAGGATGCCA+AGG 0.607478 8_3:-399438 None:intergenic
GTCCTGAAGATCTGTCATCA+AGG 0.612922 8_3:-399450 None:intergenic
GTCACTAGCAGCAAGATCAA+CGG 0.616043 8_3:-399206 None:intergenic
CATCAAGGAGGATGCCAAGG+AGG 0.643523 8_3:-399435 None:intergenic
CTAGTATCTGTAGCAAACAC+CGG 0.681554 8_3:-399514 None:intergenic
AAAGATTGTGAACAGAACAA+GGG 0.695228 8_3:-399764 None:intergenic
CTGAAGATCTGTCATCAAGG+AGG 0.709942 8_3:-399447 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTACTTGATTAAATT+TGG - chr8_3:398886-398905 None:intergenic 10.0%
!!! AAATTTAATCAAGTAATTTT+TGG + chr8_3:398885-398904 Msa1267970:intron 10.0%
!! AAAAATTACTTGATTAAATT+TGG - chr8_3:398886-398905 None:intergenic 10.0%
!!! AAATTTAATCAAGTAATTTT+TGG + chr8_3:398885-398904 Msa1267970:intron 10.0%
!! TTCTATTCGTTATAATTTAT+CGG + chr8_3:398813-398832 Msa1267970:CDS 15.0%
!! TTAAATATTGATACTTAACT+TGG + chr8_3:399110-399129 Msa1267970:intron 15.0%
!! TATTACATTGAAAACATAAA+CGG - chr8_3:399540-399559 None:intergenic 15.0%
!!! TTACATCTTACATTTTTAAT+TGG + chr8_3:399696-399715 Msa1267970:three_prime_UTR 15.0%
!! TTCTATTCGTTATAATTTAT+CGG + chr8_3:398813-398832 Msa1267970:CDS 15.0%
!! TTAAATATTGATACTTAACT+TGG + chr8_3:399110-399129 Msa1267970:intron 15.0%
!! TATTACATTGAAAACATAAA+CGG - chr8_3:399540-399559 None:intergenic 15.0%
!!! TTACATCTTACATTTTTAAT+TGG + chr8_3:399696-399715 Msa1267970:three_prime_UTR 15.0%
!! TTATAGATATAGATATGTTG+AGG + chr8_3:398952-398971 Msa1267970:CDS 20.0%
!! TATAGATATAGATATGTTGA+GGG + chr8_3:398953-398972 Msa1267970:CDS 20.0%
!! TTATAGATATAGATATGTTG+AGG + chr8_3:398952-398971 Msa1267970:CDS 20.0%
!! TATAGATATAGATATGTTGA+GGG + chr8_3:398953-398972 Msa1267970:CDS 20.0%
!!! AAGAGACATATTTTTCAAGA+AGG - chr8_3:398795-398814 None:intergenic 25.0%
!!! AGTAATTTTTGGCATCTATT+TGG + chr8_3:398896-398915 Msa1267970:intron 25.0%
!! CTATAATATCTGAAATTCCA+TGG - chr8_3:398938-398957 None:intergenic 25.0%
!! TAATCTTCTCTTTTCTTTCT+TGG + chr8_3:398996-399015 Msa1267970:intron 25.0%
!! AATCTTCTCTTTTCTTTCTT+GGG + chr8_3:398997-399016 Msa1267970:intron 25.0%
!! TCTATCCTTTTATATTTCCA+GGG + chr8_3:399133-399152 Msa1267970:intron 25.0%
! GGAAAGATATTCTATTGATA+AGG - chr8_3:399231-399250 None:intergenic 25.0%
! TATCAATAGAATATCTTTCC+AGG + chr8_3:399231-399250 Msa1267970:CDS 25.0%
! AGTATAATATAAGGATTACC+TGG - chr8_3:399252-399271 None:intergenic 25.0%
! AGGTAATCCTTATATTATAC+TGG + chr8_3:399251-399270 Msa1267970:intron 25.0%
! TTTGTGTCCAGTATAATATA+AGG - chr8_3:399261-399280 None:intergenic 25.0%
!!! ACATACAATTTTAGAGGATT+CGG - chr8_3:399475-399494 None:intergenic 25.0%
!! TACAGAACATACAATTTTAG+AGG - chr8_3:399481-399500 None:intergenic 25.0%
!! TTAAGGTTGCTAATATGTTT+TGG + chr8_3:399672-399691 Msa1267970:three_prime_UTR 25.0%
!!! CATTTTTAATTGGTCAATAC+TGG + chr8_3:399706-399725 Msa1267970:three_prime_UTR 25.0%
! GAATCATGAATAAAAAGAGT+TGG + chr8_3:399825-399844 Msa1267970:three_prime_UTR 25.0%
!!! AAGAGACATATTTTTCAAGA+AGG - chr8_3:398795-398814 None:intergenic 25.0%
!!! AGTAATTTTTGGCATCTATT+TGG + chr8_3:398896-398915 Msa1267970:intron 25.0%
!! CTATAATATCTGAAATTCCA+TGG - chr8_3:398938-398957 None:intergenic 25.0%
!! TAATCTTCTCTTTTCTTTCT+TGG + chr8_3:398996-399015 Msa1267970:intron 25.0%
!! AATCTTCTCTTTTCTTTCTT+GGG + chr8_3:398997-399016 Msa1267970:intron 25.0%
!! TCTATCCTTTTATATTTCCA+GGG + chr8_3:399133-399152 Msa1267970:intron 25.0%
! GGAAAGATATTCTATTGATA+AGG - chr8_3:399231-399250 None:intergenic 25.0%
! TATCAATAGAATATCTTTCC+AGG + chr8_3:399231-399250 Msa1267970:CDS 25.0%
! AGTATAATATAAGGATTACC+TGG - chr8_3:399252-399271 None:intergenic 25.0%
! AGGTAATCCTTATATTATAC+TGG + chr8_3:399251-399270 Msa1267970:intron 25.0%
! TTTGTGTCCAGTATAATATA+AGG - chr8_3:399261-399280 None:intergenic 25.0%
!!! ACATACAATTTTAGAGGATT+CGG - chr8_3:399475-399494 None:intergenic 25.0%
!! TACAGAACATACAATTTTAG+AGG - chr8_3:399481-399500 None:intergenic 25.0%
!! TTAAGGTTGCTAATATGTTT+TGG + chr8_3:399672-399691 Msa1267970:three_prime_UTR 25.0%
!!! CATTTTTAATTGGTCAATAC+TGG + chr8_3:399706-399725 Msa1267970:three_prime_UTR 25.0%
! GAATCATGAATAAAAAGAGT+TGG + chr8_3:399825-399844 Msa1267970:three_prime_UTR 25.0%
!!! CATTTTTTGGTGCTTTAGTA+TGG + chr8_3:398695-398714 Msa1267970:CDS 30.0%
! GTTTTCTCTTCTGTCAAAAT+GGG + chr8_3:398717-398736 Msa1267970:CDS 30.0%
AAAGCATGTTCAAATGCATT+GGG - chr8_3:398771-398790 None:intergenic 30.0%
! AAAAGCATGTTCAAATGCAT+TGG - chr8_3:398772-398791 None:intergenic 30.0%
! TTCGTTATAATTTATCGGCT+AGG + chr8_3:398818-398837 Msa1267970:CDS 30.0%
! TCGTTATAATTTATCGGCTA+GGG + chr8_3:398819-398838 Msa1267970:CDS 30.0%
ATATAGATATGTTGAGGGAT+TGG + chr8_3:398958-398977 Msa1267970:CDS 30.0%
GAAAGAAAAGAGAAGATTAC+CGG - chr8_3:398995-399014 None:intergenic 30.0%
! GTCTATCCTTTTATATTTCC+AGG + chr8_3:399132-399151 Msa1267970:intron 30.0%
ATGAACCCTGGAAATATAAA+AGG - chr8_3:399141-399160 None:intergenic 30.0%
TTCTTTGTATTTGTTCTGTG+TGG + chr8_3:399309-399328 Msa1267970:intron 30.0%
! TTCAAGTTTGTCATTTAGAC+TGG + chr8_3:399617-399636 Msa1267970:three_prime_UTR 30.0%
!!! TGCTGTTGATTTTGTATCAT+AGG + chr8_3:399640-399659 Msa1267970:three_prime_UTR 30.0%
! ATCATAGGTGTACTGTTTTA+AGG + chr8_3:399655-399674 Msa1267970:three_prime_UTR 30.0%
AAAGATTGTGAACAGAACAA+GGG - chr8_3:399767-399786 None:intergenic 30.0%
AAAAGATTGTGAACAGAACA+AGG - chr8_3:399768-399787 None:intergenic 30.0%
!!! CATTTTTTGGTGCTTTAGTA+TGG + chr8_3:398695-398714 Msa1267970:CDS 30.0%
! GTTTTCTCTTCTGTCAAAAT+GGG + chr8_3:398717-398736 Msa1267970:CDS 30.0%
AAAGCATGTTCAAATGCATT+GGG - chr8_3:398771-398790 None:intergenic 30.0%
! AAAAGCATGTTCAAATGCAT+TGG - chr8_3:398772-398791 None:intergenic 30.0%
! TTCGTTATAATTTATCGGCT+AGG + chr8_3:398818-398837 Msa1267970:CDS 30.0%
! TCGTTATAATTTATCGGCTA+GGG + chr8_3:398819-398838 Msa1267970:CDS 30.0%
ATATAGATATGTTGAGGGAT+TGG + chr8_3:398958-398977 Msa1267970:CDS 30.0%
GAAAGAAAAGAGAAGATTAC+CGG - chr8_3:398995-399014 None:intergenic 30.0%
! GTCTATCCTTTTATATTTCC+AGG + chr8_3:399132-399151 Msa1267970:intron 30.0%
ATGAACCCTGGAAATATAAA+AGG - chr8_3:399141-399160 None:intergenic 30.0%
TTCTTTGTATTTGTTCTGTG+TGG + chr8_3:399309-399328 Msa1267970:intron 30.0%
! TTCAAGTTTGTCATTTAGAC+TGG + chr8_3:399617-399636 Msa1267970:three_prime_UTR 30.0%
!!! TGCTGTTGATTTTGTATCAT+AGG + chr8_3:399640-399659 Msa1267970:three_prime_UTR 30.0%
! ATCATAGGTGTACTGTTTTA+AGG + chr8_3:399655-399674 Msa1267970:three_prime_UTR 30.0%
AAAGATTGTGAACAGAACAA+GGG - chr8_3:399767-399786 None:intergenic 30.0%
AAAAGATTGTGAACAGAACA+AGG - chr8_3:399768-399787 None:intergenic 30.0%
!!! ACGAGTGGAAACTCATTTTT+TGG + chr8_3:398682-398701 Msa1267970:CDS 35.0%
! GGTTTTCTCTTCTGTCAAAA+TGG + chr8_3:398716-398735 Msa1267970:CDS 35.0%
! TATAATTTATCGGCTAGGGT+TGG + chr8_3:398823-398842 Msa1267970:intron 35.0%
GTGTAAAGCAAATTAAGCCA+TGG + chr8_3:398918-398937 Msa1267970:intron 35.0%
ATTCCTAGTTGCAAAGACAT+GGG - chr8_3:399058-399077 None:intergenic 35.0%
TAGGAATGGAAAAACGAAGA+GGG + chr8_3:399071-399090 Msa1267970:intron 35.0%
ACAAACTTAATGAGCAACGA+AGG + chr8_3:399276-399295 Msa1267970:intron 35.0%
! TCTTGTTGTGCCTGTAATAA+GGG - chr8_3:399368-399387 None:intergenic 35.0%
! ATCTTGTTGTGCCTGTAATA+AGG - chr8_3:399369-399388 None:intergenic 35.0%
TCTGTATTTAAGAGAACCTC+CGG + chr8_3:399495-399514 Msa1267970:three_prime_UTR 35.0%
!!! ACGAGTGGAAACTCATTTTT+TGG + chr8_3:398682-398701 Msa1267970:CDS 35.0%
! GGTTTTCTCTTCTGTCAAAA+TGG + chr8_3:398716-398735 Msa1267970:CDS 35.0%
! TATAATTTATCGGCTAGGGT+TGG + chr8_3:398823-398842 Msa1267970:intron 35.0%
GTGTAAAGCAAATTAAGCCA+TGG + chr8_3:398918-398937 Msa1267970:intron 35.0%
ATTCCTAGTTGCAAAGACAT+GGG - chr8_3:399058-399077 None:intergenic 35.0%
TAGGAATGGAAAAACGAAGA+GGG + chr8_3:399071-399090 Msa1267970:intron 35.0%
ACAAACTTAATGAGCAACGA+AGG + chr8_3:399276-399295 Msa1267970:intron 35.0%
! TCTTGTTGTGCCTGTAATAA+GGG - chr8_3:399368-399387 None:intergenic 35.0%
! ATCTTGTTGTGCCTGTAATA+AGG - chr8_3:399369-399388 None:intergenic 35.0%
TCTGTATTTAAGAGAACCTC+CGG + chr8_3:399495-399514 Msa1267970:three_prime_UTR 35.0%
CATTCCTAGTTGCAAAGACA+TGG - chr8_3:399059-399078 None:intergenic 40.0%
CATGTCTTTGCAACTAGGAA+TGG + chr8_3:399057-399076 Msa1267970:intron 40.0%
CTAGGAATGGAAAAACGAAG+AGG + chr8_3:399070-399089 Msa1267970:intron 40.0%
ATATTTAACTCCCTCTCTCC+CGG - chr8_3:399098-399117 None:intergenic 40.0%
! AAAAAGATCAGCACTGGAGA+TGG - chr8_3:399167-399186 None:intergenic 40.0%
!!! ATCTCCAGTGCTGATCTTTT+TGG + chr8_3:399166-399185 Msa1267970:CDS 40.0%
! TGTTCTGTGTGGATGTTTTG+TGG + chr8_3:399320-399339 Msa1267970:intron 40.0%
TCTTCCCTTAAGAACATTGC+TGG + chr8_3:399392-399411 Msa1267970:CDS 40.0%
CTCTCCAGCAATGTTCTTAA+GGG - chr8_3:399399-399418 None:intergenic 40.0%
TCTCTCCAGCAATGTTCTTA+AGG - chr8_3:399400-399419 None:intergenic 40.0%
CTAGTATCTGTAGCAAACAC+CGG - chr8_3:399517-399536 None:intergenic 40.0%
CATTCCTAGTTGCAAAGACA+TGG - chr8_3:399059-399078 None:intergenic 40.0%
CATGTCTTTGCAACTAGGAA+TGG + chr8_3:399057-399076 Msa1267970:intron 40.0%
CTAGGAATGGAAAAACGAAG+AGG + chr8_3:399070-399089 Msa1267970:intron 40.0%
ATATTTAACTCCCTCTCTCC+CGG - chr8_3:399098-399117 None:intergenic 40.0%
! AAAAAGATCAGCACTGGAGA+TGG - chr8_3:399167-399186 None:intergenic 40.0%
!!! ATCTCCAGTGCTGATCTTTT+TGG + chr8_3:399166-399185 Msa1267970:CDS 40.0%
! TGTTCTGTGTGGATGTTTTG+TGG + chr8_3:399320-399339 Msa1267970:intron 40.0%
TCTTCCCTTAAGAACATTGC+TGG + chr8_3:399392-399411 Msa1267970:CDS 40.0%
CTCTCCAGCAATGTTCTTAA+GGG - chr8_3:399399-399418 None:intergenic 40.0%
TCTCTCCAGCAATGTTCTTA+AGG - chr8_3:399400-399419 None:intergenic 40.0%
CTAGTATCTGTAGCAAACAC+CGG - chr8_3:399517-399536 None:intergenic 40.0%
! GGGATTGGTAAGCTATGTTC+CGG + chr8_3:398973-398992 Msa1267970:CDS 45.0%
TCTCCCATGTCTTTGCAACT+AGG + chr8_3:399052-399071 Msa1267970:intron 45.0%
TATTTAACTCCCTCTCTCCC+GGG - chr8_3:399097-399116 None:intergenic 45.0%
GTCACCAAAAAGATCAGCAC+TGG - chr8_3:399173-399192 None:intergenic 45.0%
GTCACTAGCAGCAAGATCAA+CGG - chr8_3:399209-399228 None:intergenic 45.0%
! TGTAATAAGGGAGGGGTGTA+GGG - chr8_3:399356-399375 None:intergenic 45.0%
TTGTGCCTGTAATAAGGGAG+GGG - chr8_3:399363-399382 None:intergenic 45.0%
GTTGTGCCTGTAATAAGGGA+GGG - chr8_3:399364-399383 None:intergenic 45.0%
TGTTGTGCCTGTAATAAGGG+AGG - chr8_3:399365-399384 None:intergenic 45.0%
AAGAACATTGCTGGAGAGAC+TGG + chr8_3:399401-399420 Msa1267970:CDS 45.0%
AGAACATTGCTGGAGAGACT+GGG + chr8_3:399402-399421 Msa1267970:CDS 45.0%
TGGGAAAAAACTCACCTCCT+TGG + chr8_3:399421-399440 Msa1267970:CDS 45.0%
CTGAAGATCTGTCATCAAGG+AGG - chr8_3:399450-399469 None:intergenic 45.0%
! CTCCTTGATGACAGATCTTC+AGG + chr8_3:399448-399467 Msa1267970:CDS 45.0%
GTCCTGAAGATCTGTCATCA+AGG - chr8_3:399453-399472 None:intergenic 45.0%
!! GTCAATACTGGTTTGTCGCA+AGG + chr8_3:399718-399737 Msa1267970:three_prime_UTR 45.0%
! GGGATTGGTAAGCTATGTTC+CGG + chr8_3:398973-398992 Msa1267970:CDS 45.0%
TCTCCCATGTCTTTGCAACT+AGG + chr8_3:399052-399071 Msa1267970:intron 45.0%
TATTTAACTCCCTCTCTCCC+GGG - chr8_3:399097-399116 None:intergenic 45.0%
GTCACCAAAAAGATCAGCAC+TGG - chr8_3:399173-399192 None:intergenic 45.0%
GTCACTAGCAGCAAGATCAA+CGG - chr8_3:399209-399228 None:intergenic 45.0%
! TGTAATAAGGGAGGGGTGTA+GGG - chr8_3:399356-399375 None:intergenic 45.0%
TTGTGCCTGTAATAAGGGAG+GGG - chr8_3:399363-399382 None:intergenic 45.0%
GTTGTGCCTGTAATAAGGGA+GGG - chr8_3:399364-399383 None:intergenic 45.0%
TGTTGTGCCTGTAATAAGGG+AGG - chr8_3:399365-399384 None:intergenic 45.0%
AAGAACATTGCTGGAGAGAC+TGG + chr8_3:399401-399420 Msa1267970:CDS 45.0%
AGAACATTGCTGGAGAGACT+GGG + chr8_3:399402-399421 Msa1267970:CDS 45.0%
TGGGAAAAAACTCACCTCCT+TGG + chr8_3:399421-399440 Msa1267970:CDS 45.0%
CTGAAGATCTGTCATCAAGG+AGG - chr8_3:399450-399469 None:intergenic 45.0%
! CTCCTTGATGACAGATCTTC+AGG + chr8_3:399448-399467 Msa1267970:CDS 45.0%
GTCCTGAAGATCTGTCATCA+AGG - chr8_3:399453-399472 None:intergenic 45.0%
!! GTCAATACTGGTTTGTCGCA+AGG + chr8_3:399718-399737 Msa1267970:three_prime_UTR 45.0%
ATGGAAAAACGAAGAGGGCC+CGG + chr8_3:399076-399095 Msa1267970:intron 50.0%
! CTGTAATAAGGGAGGGGTGT+AGG - chr8_3:399357-399376 None:intergenic 50.0%
CTACACCCCTCCCTTATTAC+AGG + chr8_3:399355-399374 Msa1267970:intron 50.0%
TGTCATCAAGGAGGATGCCA+AGG - chr8_3:399441-399460 None:intergenic 50.0%
GTATCTGTAGCAAACACCGG+AGG - chr8_3:399514-399533 None:intergenic 50.0%
ATGGAAAAACGAAGAGGGCC+CGG + chr8_3:399076-399095 Msa1267970:intron 50.0%
! CTGTAATAAGGGAGGGGTGT+AGG - chr8_3:399357-399376 None:intergenic 50.0%
CTACACCCCTCCCTTATTAC+AGG + chr8_3:399355-399374 Msa1267970:intron 50.0%
TGTCATCAAGGAGGATGCCA+AGG - chr8_3:399441-399460 None:intergenic 50.0%
GTATCTGTAGCAAACACCGG+AGG - chr8_3:399514-399533 None:intergenic 50.0%
!! ATCGGCTAGGGTTGGTTGAG+TGG + chr8_3:398831-398850 Msa1267970:intron 55.0%
!! TCGGCTAGGGTTGGTTGAGT+GGG + chr8_3:398832-398851 Msa1267970:intron 55.0%
GAGTGGGATGAGTGCGAGAA+AGG + chr8_3:398848-398867 Msa1267970:intron 55.0%
TGGAAAAACGAAGAGGGCCC+GGG + chr8_3:399077-399096 Msa1267970:intron 55.0%
TGGAGATGGCAGATGAACCC+TGG - chr8_3:399153-399172 None:intergenic 55.0%
CATCAAGGAGGATGCCAAGG+AGG - chr8_3:399438-399457 None:intergenic 55.0%
!! ATCGGCTAGGGTTGGTTGAG+TGG + chr8_3:398831-398850 Msa1267970:intron 55.0%
!! TCGGCTAGGGTTGGTTGAGT+GGG + chr8_3:398832-398851 Msa1267970:intron 55.0%
GAGTGGGATGAGTGCGAGAA+AGG + chr8_3:398848-398867 Msa1267970:intron 55.0%
TGGAAAAACGAAGAGGGCCC+GGG + chr8_3:399077-399096 Msa1267970:intron 55.0%
TGGAGATGGCAGATGAACCC+TGG - chr8_3:399153-399172 None:intergenic 55.0%
CATCAAGGAGGATGCCAAGG+AGG - chr8_3:399438-399457 None:intergenic 55.0%
ACGAAGAGGGCCCGGGAGAG+AGG + chr8_3:399084-399103 Msa1267970:intron 70.0%
CGAAGAGGGCCCGGGAGAGA+GGG + chr8_3:399085-399104 Msa1267970:intron 70.0%
ACGAAGAGGGCCCGGGAGAG+AGG + chr8_3:399084-399103 Msa1267970:intron 70.0%
CGAAGAGGGCCCGGGAGAGA+GGG + chr8_3:399085-399104 Msa1267970:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 398676 399853 398676 ID=Msa1267970;Name=Msa1267970
chr8_3 mRNA 398676 399853 398676 ID=Msa1267970-mRNA-1;Parent=Msa1267970;Name=Msa1267970-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0.33|0.25|1|1|1|4|369|146
chr8_3 exon 398676 398840 398676 ID=Msa1267970-mRNA-1:exon:74;Parent=Msa1267970-mRNA-1
chr8_3 exon 398926 398994 398926 ID=Msa1267970-mRNA-1:exon:75;Parent=Msa1267970-mRNA-1
chr8_3 exon 399154 399252 399154 ID=Msa1267970-mRNA-1:exon:76;Parent=Msa1267970-mRNA-1
chr8_3 exon 399377 399853 399377 ID=Msa1267970-mRNA-1:exon:77;Parent=Msa1267970-mRNA-1
chr8_3 CDS 398676 398840 398676 ID=Msa1267970-mRNA-1:cds;Parent=Msa1267970-mRNA-1
chr8_3 CDS 398926 398994 398926 ID=Msa1267970-mRNA-1:cds;Parent=Msa1267970-mRNA-1
chr8_3 CDS 399154 399252 399154 ID=Msa1267970-mRNA-1:cds;Parent=Msa1267970-mRNA-1
chr8_3 CDS 399377 399484 399377 ID=Msa1267970-mRNA-1:cds;Parent=Msa1267970-mRNA-1
chr8_3 three_prime_UTR 399485 399853 399485 ID=Msa1267970-mRNA-1:three_prime_utr;Parent=Msa1267970-mRNA-1
Gene Sequence

>Msa1267970

ATGTTTACGAGTGGAAACTCATTTTTTGGTGCTTTAGTATGGTTTTCTCTTCTGTCAAAATGGGTTGTAGTGCATCAATTTACAGAATTTAACCCAATGCATTTGAACATGCTTTTCCTTCTTGAAAAATATGTCTCTTCTATTCGTTATAATTTATCGGCTAGGCAAATTAAGCCATGGAATTTCAGATATTATAGATATAGATATGTTGAGGGATTGGTAAGCTATGTTCCGGGTTCATCTGCCATCTCCAGTGCTGATCTTTTTGGTGACTCGAGTGACAACTCCGTTGATCTTGCTGCTAGTGACCTTATCAATAGAATATCTTTCCAGGCACAACAAGATATCTCTTCCCTTAAGAACATTGCTGGAGAGACTGGGAAAAAACTCACCTCCTTGGCATCCTCCTTGATGACAGATCTTCAGGACCGAATCCTCTAA
Protein sequence

>Msa1267970

MFTSGNSFFGALVWFSLLSKWVVVHQFTEFNPMHLNMLFLLEKYVSSIRYNLSARQIKPWNFRYYRYRYVEGLVSYVPGSSAISSADLFGDSSDNSVDLAASDLINRISFQAQQDISSLKNIAGETGKKLTSLASSLMTDLQDRIL*