AlfalfaGEDB | Msa1271030

M. sativa cultivar zhongmu-4 / Msa1271030

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007049 GO:0007275 GO:0008150 GO:0009553 GO:0009554 GO:0009555 GO:0009556 GO:0009791 GO:0009908 GO:0009987 GO:0019953 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0034293 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043934 GO:0044424 GO:0044464 GO:0044703 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0051321 GO:0051704 GO:0061458 GO:0090567 GO:0097159 GO:0099402 GO:1901363 GO:1903046
PFAM: PF03110.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1271030	XP_003626693.1	93.711	318	11	4	1	316	1	311	0.0	582

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1271030	sp\|Q8GXL3\|SPL8_ARATH	44.611	334	145	10	1	316	1	312	3.80e-78	245

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1271030	G7LBJ7	93.711	318	11	4	1	316	1	311	0.0	582

TFs/TRs:

Gene ID	Type	Classification
Msa1271030	TF	SBP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0799030	Msa1271030	0.807662	4.530981e-50	-8.615850e-47
Msa1227950	Msa1271030	0.852531	4.343224e-61	-8.615850e-47
Msa1227970	Msa1271030	0.852783	3.679410e-61	-8.615850e-47
Msa1271030	Msa1308130	0.882081	1.404357e-70	-8.615850e-47
Msa1271030	Msa1339940	0.811612	6.417775e-51	-8.615850e-47
Msa1271030	Msa1452060	0.804588	2.009633e-49	-8.615850e-47
Msa0479680	Msa1271030	0.808205	3.472591e-50	-8.615850e-47
Msa0479700	Msa1271030	0.806512	7.936354e-50	-8.615850e-47
Msa0565600	Msa1271030	0.807100	5.960483e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1271030	MtrunA17_Chr8g0334671	93.711	318	11	4	1	316	1	311	0.0	582
Msa1271030	MtrunA17_Chr8g0360361	70.000	90	26	1	190	279	63	151	1.16e-35	127
Msa1271030	MtrunA17_Chr4g0046991	72.619	84	22	1	184	266	64	147	2.08e-35	126
Msa1271030	MtrunA17_Chr2g0302791	52.419	124	53	2	152	275	104	221	7.20e-35	135
Msa1271030	MtrunA17_Chr1g0194121	56.522	115	41	1	152	266	116	221	7.71e-35	134
Msa1271030	MtrunA17_Chr7g0231911	67.857	84	27	0	185	268	142	225	1.19e-34	129
Msa1271030	MtrunA17_Chr3g0122411	54.472	123	49	2	152	267	118	240	6.97e-34	129
Msa1271030	MtrunA17_Chr2g0282521	67.470	83	27	0	184	266	56	138	2.18e-33	120
Msa1271030	MtrunA17_Chr4g0061651	70.370	81	24	0	187	267	118	198	2.50e-33	128
Msa1271030	MtrunA17_Chr7g0270541	49.624	133	59	2	134	266	95	219	2.70e-33	130
Msa1271030	MtrunA17_Chr2g0308321	72.368	76	21	0	192	267	171	246	2.93e-33	129
Msa1271030	MtrunA17_Chr7g0258471	67.857	84	27	0	182	265	132	215	3.37e-33	127
Msa1271030	MtrunA17_Chr5g0420051	61.458	96	33	1	172	267	165	256	3.43e-33	127
Msa1271030	MtrunA17_Chr1g0162871	55.000	120	43	1	148	267	71	179	7.02e-33	129
Msa1271030	MtrunA17_Chr8g0386271	55.752	113	46	1	155	267	66	174	7.73e-32	122
Msa1271030	MtrunA17_Chr3g0132091	68.421	76	24	0	192	267	73	148	2.40e-30	118
Msa1271030	MtrunA17_Chr7g0224821	62.651	83	31	0	187	269	72	154	8.22e-30	117
Msa1271030	MtrunA17_Chr8g0377401	50.442	113	51	2	152	264	127	234	9.34e-28	108
Msa1271030	MtrunA17_Chr8g0377421	45.902	122	53	2	156	265	85	205	8.51e-26	104
Msa1271030	MtrunA17_Chr2g0286661	58.108	74	31	0	192	265	103	176	6.67e-24	102
Msa1271030	MtrunA17_Chr2g0317041	66.102	59	20	0	180	238	87	145	2.32e-21	89.0
Msa1271030	MtrunA17_Chr1g0175031	43.434	99	43	1	153	238	22	120	6.34e-19	81.6
Msa1271030	MtrunA17_Chr8g0377431	57.143	56	24	0	190	245	149	204	1.29e-15	74.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1271030	AT1G02065.1	44.611	334	145	10	1	316	1	312	3.86e-79	245
Msa1271030	AT1G02065.2	43.600	250	115	6	1	239	1	235	9.68e-58	187
Msa1271030	AT1G69170.1	53.543	127	52	3	139	265	78	197	1.90e-34	130
Msa1271030	AT1G20980.1	57.627	118	49	1	152	269	81	197	3.33e-34	133
Msa1271030	AT1G27370.6	47.368	152	59	3	132	267	105	251	4.46e-34	128
Msa1271030	AT1G27370.1	47.368	152	59	3	132	267	105	251	6.82e-34	129
Msa1271030	AT1G27370.5	47.368	152	59	3	132	267	105	251	6.82e-34	129
Msa1271030	AT1G27370.3	47.368	152	59	3	132	267	105	251	6.82e-34	129
Msa1271030	AT1G27370.2	47.368	152	59	3	132	267	105	251	6.82e-34	129
Msa1271030	AT1G27370.4	47.368	152	59	3	132	267	105	251	6.82e-34	129
Msa1271030	AT1G27370.7	47.368	152	59	3	132	267	105	251	6.82e-34	129
Msa1271030	AT2G47070.1	73.333	75	20	0	192	266	106	180	2.59e-33	130
Msa1271030	AT3G60030.1	54.545	121	44	3	151	271	96	205	2.91e-33	130
Msa1271030	AT1G76580.2	71.250	80	23	0	188	267	110	189	3.25e-33	130
Msa1271030	AT1G76580.3	71.250	80	23	0	188	267	110	189	3.39e-33	130
Msa1271030	AT2G33810.1	67.073	82	27	0	185	266	47	128	5.16e-33	119
Msa1271030	AT1G76580.1	71.250	80	23	0	188	267	110	189	5.22e-33	130
Msa1271030	AT5G43270.3	53.125	128	43	3	152	265	118	242	1.28e-32	125
Msa1271030	AT5G43270.1	53.125	128	43	3	152	265	118	242	1.28e-32	125
Msa1271030	AT5G43270.2	53.125	128	43	3	152	265	118	242	1.28e-32	125
Msa1271030	AT1G53160.3	60.606	99	35	2	177	271	35	133	2.56e-32	119
Msa1271030	AT1G53160.2	62.766	94	31	2	182	271	40	133	4.25e-32	118
Msa1271030	AT1G53160.1	62.766	94	31	2	182	271	40	133	4.25e-32	118
Msa1271030	AT3G57920.1	56.140	114	44	2	154	267	27	134	6.84e-32	122
Msa1271030	AT1G27360.2	50.781	128	53	2	150	267	123	250	3.46e-31	121
Msa1271030	AT1G27360.1	50.781	128	53	2	150	267	123	250	3.46e-31	121
Msa1271030	AT1G27360.3	50.781	128	53	2	150	267	123	250	3.46e-31	121
Msa1271030	AT1G27360.4	50.781	128	53	2	150	267	123	250	3.46e-31	121
Msa1271030	AT5G50570.1	60.714	84	33	0	184	267	93	176	5.36e-30	117
Msa1271030	AT5G50570.2	60.714	84	33	0	184	267	93	176	5.36e-30	117
Msa1271030	AT5G50670.1	60.714	84	33	0	184	267	93	176	5.36e-30	117
Msa1271030	AT5G50670.2	60.714	84	33	0	184	267	93	176	5.36e-30	117
Msa1271030	AT2G42200.1	66.667	78	26	0	190	267	72	149	1.11e-29	117
Msa1271030	AT3G15270.1	62.667	75	28	0	192	266	63	137	6.44e-29	110
Msa1271030	AT5G18830.2	46.316	95	51	0	171	265	117	211	2.21e-22	98.6
Msa1271030	AT5G18830.1	46.316	95	51	0	171	265	117	211	2.63e-22	98.2
Msa1271030	AT5G18830.3	46.316	95	51	0	171	265	117	211	2.88e-22	98.2
Msa1271030	AT2G47070.2	72.549	51	14	0	216	266	1	51	8.29e-19	87.8

Find 155 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCATTAATTAATTTCTTCT+TGG	0.199513	8_3:+4411565	None:intergenic
CAAATTAAATTAAATTAAAC+TGG	0.232500	8_3:+4411616	None:intergenic
TTTGTTTCTGCTATGCTAAA+TGG	0.234964	8_3:+4413880	None:intergenic
GCTTAAAATGAAATTAATAT+TGG	0.246621	8_3:+4411470	None:intergenic
TTGTGAATATCTTGAGTATT+TGG	0.246735	8_3:+4412024	None:intergenic
CTGTTAACATCCTCGGATTT+TGG	0.256271	8_3:+4413118	None:intergenic
GGAGTTGTTGTCTTCATTTC+CGG	0.269715	8_3:+4413480	None:intergenic
CTCTCCCTTGACCCTCTTCC+TGG	0.270502	8_3:-4413172	Msa1271030:CDS
TCCAAGAAGAAATTAATTAA+TGG	0.276446	8_3:-4411566	Msa1271030:three_prime_UTR
CTTCTATCTGAGTTTGATAA+TGG	0.278524	8_3:-4412108	Msa1271030:CDS
CTTGAAGTCGTTGAAGGGCT+TGG	0.291934	8_3:+4411719	None:intergenic
ATCACAACCTGCTGCATTGC+TGG	0.295297	8_3:+4412825	None:intergenic
CCTAATTTGCAGAAAAGTTA+TGG	0.306031	8_3:+4413679	None:intergenic
TCTAGAGCCCACGAAGTCTA+TGG	0.307240	8_3:+4413093	None:intergenic
CCGGTAAGCATGATGTTTGT+TGG	0.322804	8_3:+4413499	None:intergenic
GCAGCCACCGTCGTTGCAGC+TGG	0.327647	8_3:-4412866	Msa1271030:CDS
CTTAAAATGAAATTAATATT+GGG	0.344072	8_3:+4411471	None:intergenic
CCAACAAACATCATGCTTAC+CGG	0.358315	8_3:-4413499	Msa1271030:CDS
GGCTAAACTGGTTGTGGTGG+TGG	0.358538	8_3:+4413308	None:intergenic
TTGAAGTCGTTGAAGGGCTT+GGG	0.360837	8_3:+4411720	None:intergenic
TGTGAATATCTTGAGTATTT+GGG	0.361532	8_3:+4412025	None:intergenic
TTGTTTCTGCTATGCTAAAT+GGG	0.369619	8_3:+4413881	None:intergenic
TGAAATTAATATTGGGAGAA+AGG	0.370310	8_3:+4411478	None:intergenic
GAAGGATCCCCGCCAGAGTC+TGG	0.374246	8_3:-4411815	Msa1271030:intron
TCGGTCTAGACCACCTGAAC+CGG	0.380874	8_3:-4413002	Msa1271030:CDS
GGAGGTTGTGGTTGAGGTGG+AGG	0.381025	8_3:+4413202	None:intergenic
TTTGCAGAAAAGTTATGGTT+GGG	0.382180	8_3:+4413684	None:intergenic
GAACCTGTCGAACCCGGTTC+AGG	0.386932	8_3:+4412989	None:intergenic
ATGGAAGGAGGTTGTGGTTG+AGG	0.388721	8_3:+4413196	None:intergenic
TACAAGAGTAACTTTCCTAT+TGG	0.389199	8_3:-4413979	Msa1271030:five_prime_UTR
TTATCCGACAACTACCTTAA+CGG	0.399307	8_3:-4413391	Msa1271030:CDS
AATTTGACGGTGGGCTTGAT+CGG	0.400326	8_3:+4413441	None:intergenic
TCGTTGAAGGGCTTGGGCTT+TGG	0.400465	8_3:+4411726	None:intergenic
CGGTAAGCATGATGTTTGTT+GGG	0.413507	8_3:+4413500	None:intergenic
CCTGGTCATGGACATGGCCC+TGG	0.414046	8_3:-4413154	Msa1271030:CDS
TACCTTAACGGACCTGGTGC+TGG	0.415148	8_3:-4413379	Msa1271030:CDS
TGCAACGACGGTGGCTGCCT+TGG	0.421484	8_3:+4412871	None:intergenic
TTACTACTTGTTAGTAAGAA+AGG	0.422025	8_3:-4411425	Msa1271030:three_prime_UTR
CCTCATCTCTCTCTACCAAT+AGG	0.422136	8_3:+4413964	None:intergenic
TAAACTGGTTGTGGTGGTGG+TGG	0.427802	8_3:+4413311	None:intergenic
CGAAGAAGGACTGATGGATA+TGG	0.430604	8_3:+4413269	None:intergenic
GAGAGCATGGAAGGAGGTTG+TGG	0.432257	8_3:+4413190	None:intergenic
TCACAAATCTCACAATACTT+TGG	0.434049	8_3:-4412007	Msa1271030:CDS
GGTAGAGAGAGATGAGGAGA+AGG	0.435900	8_3:-4413958	Msa1271030:five_prime_UTR
CCTCTTCCTGGTCATGGACA+TGG	0.445756	8_3:-4413160	Msa1271030:CDS
CAAAATCCGAGGATGTTAAC+AGG	0.446882	8_3:-4413117	Msa1271030:CDS
ACTGATGGATATGGTGCTGC+TGG	0.447816	8_3:+4413278	None:intergenic
GACAGTGGCGGTTTCTCCAC+CGG	0.454790	8_3:-4411771	Msa1271030:CDS
ATTGAGCTTGAAGTCGTTGA+AGG	0.455406	8_3:+4411713	None:intergenic
TATGGTGCTGCTGGGCCTGT+GGG	0.461581	8_3:+4413287	None:intergenic
GTCCAGCACCAGGTCCGTTA+AGG	0.461905	8_3:+4413377	None:intergenic
CTTGACCCTCTTCCTGGTCA+TGG	0.462725	8_3:-4413166	Msa1271030:CDS
GAGACATGTTGGACTATGAA+TGG	0.466941	8_3:-4413528	Msa1271030:exon
ATGTTATTTAAATTAAATGA+AGG	0.473209	8_3:+4411395	None:intergenic
TTAATTTAATTTAATTTGCA+TGG	0.478477	8_3:-4411611	Msa1271030:three_prime_UTR
ATATGGTGCTGCTGGGCCTG+TGG	0.478517	8_3:+4413286	None:intergenic
TTTGGTACGAGGAGGAATCC+AGG	0.482460	8_3:+4413136	None:intergenic
GACTTCGTGGGCTCTAGACT+TGG	0.484947	8_3:-4413088	Msa1271030:CDS
CCACCTGAACCGGGTTCGAC+AGG	0.485330	8_3:-4412992	Msa1271030:CDS
ATCGGAGGCTGCTGCTGCGG+CGG	0.485623	8_3:+4413459	None:intergenic
TTGCAATGCTGATCTATCTC+AGG	0.487614	8_3:-4412936	Msa1271030:CDS
AGACATGTTGGACTATGAAT+GGG	0.488109	8_3:-4413527	Msa1271030:exon
TATCAAACTCAGATAGAAGA+TGG	0.491198	8_3:+4412112	None:intergenic
GGTTCTGCCAGCAATGCAGC+AGG	0.491828	8_3:-4412832	Msa1271030:intron
ATTTGCAGAAAAGTTATGGT+TGG	0.492281	8_3:+4413683	None:intergenic
GGGTCAAGGGAGAGCATGGA+AGG	0.493307	8_3:+4413181	None:intergenic
ATTGAGTTCCAGACTCTGGC+GGG	0.495092	8_3:+4411807	None:intergenic
TAGATCAGCATTGCAACCTT+CGG	0.495851	8_3:+4412943	None:intergenic
ACCCACGGGCTTTCCATGGT+GGG	0.498666	8_3:-4413246	Msa1271030:CDS
GCGGCTCACGAAGTCGTCTT+CGG	0.499397	8_3:+4413033	None:intergenic
ATCAGTCCTTCTTCGACCCA+CGG	0.500041	8_3:-4413261	Msa1271030:CDS
TTCTTTCTAGAGAGACATGT+TGG	0.501123	8_3:-4413539	Msa1271030:exon
CACAAACTTTGTGGCGGCGG+TGG	0.502474	8_3:+4412903	None:intergenic
TTGCAGAAAAGTTATGGTTG+GGG	0.507975	8_3:+4413685	None:intergenic
AAGAGGGTCAAGGGAGAGCA+TGG	0.508675	8_3:+4413177	None:intergenic
GAAGGAGGTTGTGGTTGAGG+TGG	0.517345	8_3:+4413199	None:intergenic
TCAGTCCTTCTTCGACCCAC+GGG	0.517839	8_3:-4413260	Msa1271030:CDS
GACCCACGGGCTTTCCATGG+TGG	0.520528	8_3:-4413247	Msa1271030:CDS
GTGGTCTAGACCGACGGTAA+AGG	0.522892	8_3:+4413011	None:intergenic
CCAATGTGTAGCTATTAACT+AGG	0.525271	8_3:-4411654	Msa1271030:three_prime_UTR
GAAAACAGTGTGTAGTGTAA+TGG	0.528269	8_3:+4413762	None:intergenic
CCTGTGGGCTAAACTGGTTG+TGG	0.528281	8_3:+4413302	None:intergenic
AGTGAAACTCACAAACTTTG+TGG	0.531020	8_3:+4412894	None:intergenic
TCTAGACTTGGACTCAACCT+TGG	0.532282	8_3:-4413076	Msa1271030:CDS
ACGTAGCTGCAGGAAGAGAT+TGG	0.533276	8_3:-4412082	Msa1271030:CDS
CAGGTTCTTCGAACTCGCCT+CGG	0.534022	8_3:-4412973	Msa1271030:CDS
TCGATGGTGGGTCCAGCACC+AGG	0.535316	8_3:+4413367	None:intergenic
ACCGCTGTTTATGTCGATGG+TGG	0.537005	8_3:+4413354	None:intergenic
AGGTCCGTTAAGGTAGTTGT+CGG	0.538800	8_3:+4413387	None:intergenic
ATCTACATCGTCGGTGACAG+TGG	0.539420	8_3:-4411786	Msa1271030:CDS
GACCTTTGTCGAAAATAATC+CGG	0.539450	8_3:+4411752	None:intergenic
TTGATCGGAGGCTGCTGCTG+CGG	0.542405	8_3:+4413456	None:intergenic
TAACAGGCCCATAGACTTCG+TGG	0.543723	8_3:-4413101	Msa1271030:CDS
ATCACCGCTGTTTATGTCGA+TGG	0.546681	8_3:+4413351	None:intergenic
GTGGGCTAAACTGGTTGTGG+TGG	0.546991	8_3:+4413305	None:intergenic
TATGGGCCTGTTAACATCCT+CGG	0.547316	8_3:+4413111	None:intergenic
AGTCAACCCAGCTGCAACGA+CGG	0.547853	8_3:+4412859	None:intergenic
CCATGTCCATGACCAGGAAG+AGG	0.548501	8_3:+4413160	None:intergenic
CAGCCACCGTCGTTGCAGCT+GGG	0.549496	8_3:-4412865	Msa1271030:CDS
CCAGGGCCATGTCCATGACC+AGG	0.549923	8_3:+4413154	None:intergenic
TGCAGAAAAGTTATGGTTGG+GGG	0.549962	8_3:+4413686	None:intergenic
ATAATGGAAAACGTAGCTGC+AGG	0.553645	8_3:-4412092	Msa1271030:CDS
CTGATGGATATGGTGCTGCT+GGG	0.553703	8_3:+4413279	None:intergenic
TTGAGCTTGAAGTCGTTGAA+GGG	0.565867	8_3:+4411714	None:intergenic
ACTCACAAACTTTGTGGCGG+CGG	0.568304	8_3:+4412900	None:intergenic
GTGGTCGAAAATTTGACGGT+GGG	0.570524	8_3:+4413432	None:intergenic
CGTAGTGGTCGAAAATTTGA+CGG	0.570580	8_3:+4413428	None:intergenic
CCGCTGTTTATGTCGATGGT+GGG	0.570806	8_3:+4413355	None:intergenic
CATGACCAGGAAGAGGGTCA+AGG	0.572330	8_3:+4413167	None:intergenic
GACAACTACCTTAACGGACC+TGG	0.572506	8_3:-4413385	Msa1271030:CDS
TCGTGAGCCGCCTTTACCGT+CGG	0.573246	8_3:-4413021	Msa1271030:CDS
GTAGATTGAGTTCCAGACTC+TGG	0.579544	8_3:+4411803	None:intergenic
AAGAAGAAATTAATTAATGG+AGG	0.580037	8_3:-4411563	Msa1271030:three_prime_UTR
GAGGTTGTGGTTGAGGTGGA+GGG	0.580574	8_3:+4413203	None:intergenic
ATGACCAGGAAGAGGGTCAA+GGG	0.581128	8_3:+4413168	None:intergenic
TTCGAAGAACCTGTCGAACC+CGG	0.581830	8_3:+4412983	None:intergenic
CCACAACCAGTTTAGCCCAC+AGG	0.583382	8_3:-4413302	Msa1271030:CDS
GTGGGTCGAAGAAGGACTGA+TGG	0.584810	8_3:+4413263	None:intergenic
CCTGTCGAACCCGGTTCAGG+TGG	0.588152	8_3:+4412992	None:intergenic
TGGCTGATCATAATCGTCGC+AGG	0.588393	8_3:-4412062	Msa1271030:CDS
CGGTCTAGACCACCTGAACC+GGG	0.589283	8_3:-4413001	Msa1271030:CDS
AGAAGTATGTGCGGCCACCA+AGG	0.589908	8_3:+4413059	None:intergenic
CCTAGTTAATAGCTACACAT+TGG	0.594646	8_3:+4411654	None:intergenic
CCTATTGGTAGAGAGAGATG+AGG	0.594887	8_3:-4413964	Msa1271030:five_prime_UTR
AGACTTGGACTCAACCTTGG+TGG	0.599617	8_3:-4413073	Msa1271030:CDS
TTGACGGTGGGCTTGATCGG+AGG	0.603910	8_3:+4413444	None:intergenic
AAATTAAATTAAATTAAACT+GGG	0.605549	8_3:+4411617	None:intergenic
TTGCAGCTGGGTTGACTCAG+CGG	0.610713	8_3:-4412853	Msa1271030:CDS
TCAAGGGAGAGCATGGAAGG+AGG	0.612363	8_3:+4413184	None:intergenic
TTGGTACGAGGAGGAATCCA+GGG	0.615728	8_3:+4413137	None:intergenic
GTCTAGACCGACGGTAAAGG+CGG	0.615862	8_3:+4413014	None:intergenic
TAATTAATGGAGGAGCGTCA+CGG	0.616625	8_3:-4411553	Msa1271030:three_prime_UTR
CATGTCCATGACCAGGAAGA+GGG	0.618176	8_3:+4413161	None:intergenic
AGTTTGTGAGTTTCACTCCA+AGG	0.619014	8_3:-4412888	Msa1271030:CDS
TACATCGTCGGTGACAGTGG+CGG	0.620396	8_3:-4411783	Msa1271030:CDS
GATTGAGTTCCAGACTCTGG+CGG	0.626944	8_3:+4411806	None:intergenic
AAGCAGTTGATGACCCACCA+TGG	0.631764	8_3:+4413233	None:intergenic
TGGAACTCAATCTACATCGT+CGG	0.632089	8_3:-4411795	Msa1271030:CDS
CAACCCAGCTGCAACGACGG+TGG	0.640932	8_3:+4412862	None:intergenic
GTTCAGGTGGTCTAGACCGA+CGG	0.647783	8_3:+4413005	None:intergenic
AACAGGCCCATAGACTTCGT+GGG	0.651739	8_3:-4413100	Msa1271030:CDS
GCGTACGTAGGTTCACTACC+CGG	0.662915	8_3:+4411679	None:intergenic
CCTCCTCGTACCAAAATCCG+AGG	0.665794	8_3:-4413128	Msa1271030:CDS
ACCCACCATGGAAAGCCCGT+GGG	0.668555	8_3:+4413245	None:intergenic
AGTGGTCGAAAATTTGACGG+TGG	0.671593	8_3:+4413431	None:intergenic
ACATAGCTTGAGAAGCGTAG+TGG	0.674169	8_3:+4413413	None:intergenic
TACACATTGGTTGCGTACGT+AGG	0.676582	8_3:+4411667	None:intergenic
GACATGTTGGACTATGAATG+GGG	0.677368	8_3:-4413526	Msa1271030:CDS
GAAACTCACAAACTTTGTGG+CGG	0.681714	8_3:+4412897	None:intergenic
CTTTGTCGAAAATAATCCGG+TGG	0.682961	8_3:+4411755	None:intergenic
CAACCTTCGGCCTGACACCG+AGG	0.715496	8_3:+4412956	None:intergenic
TTGAGTTCCAGACTCTGGCG+GGG	0.723001	8_3:+4411808	None:intergenic
CTTCGGAAGAGAAGTATGTG+CGG	0.729099	8_3:+4413050	None:intergenic
CCCACCATCGACATAAACAG+CGG	0.746666	8_3:-4413355	Msa1271030:CDS
TACGTAGGTTCACTACCCGG+TGG	0.763199	8_3:+4411682	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATAAATAAATATATATGC+AGG	-	chr8_3:4413280-4413299	Msa1271030:CDS	10.0%
!!	CAAATTAAATTAAATTAAAC+TGG	+	chr8_3:4413804-4413823	None:intergenic	10.0%
!!!	TTAATTTAATTTAATTTGCA+TGG	-	chr8_3:4413806-4413825	Msa1271030:five_prime_UTR	10.0%
!!	CTTAAAATGAAATTAATATT+GGG	+	chr8_3:4413949-4413968	None:intergenic	10.0%
!!!	TAAAAGTCATTCTTTATTTT+TGG	+	chr8_3:4411793-4411812	None:intergenic	15.0%
!!	ATTCTATTTAACCAAAAATA+AGG	-	chr8_3:4413526-4413545	Msa1271030:CDS	15.0%
!!	TTCTATTTAACCAAAAATAA+GGG	-	chr8_3:4413527-4413546	Msa1271030:exon	15.0%
!!!	TGATTTGTTTTTTATTTTGA+AGG	-	chr8_3:4413584-4413603	Msa1271030:intron	15.0%
!!	GCTTAAAATGAAATTAATAT+TGG	+	chr8_3:4413950-4413969	None:intergenic	15.0%
!!!	TGAGTTAGGTTTTAATTAAA+CGG	-	chr8_3:4412785-4412804	Msa1271030:intron	20.0%
!!	AAAATACACATCTTAATCAA+AGG	+	chr8_3:4412849-4412868	None:intergenic	20.0%
!!!	TTTGATTAAGATGTGTATTT+TGG	-	chr8_3:4412848-4412867	Msa1271030:CDS	20.0%
!!!	TTGATTAAGATGTGTATTTT+GGG	-	chr8_3:4412849-4412868	Msa1271030:CDS	20.0%
!!	TTGATTAAGAAAATTACTTG+TGG	+	chr8_3:4413445-4413464	None:intergenic	20.0%
!!	TCCAAGAAGAAATTAATTAA+TGG	-	chr8_3:4413851-4413870	Msa1271030:five_prime_UTR	20.0%
!!	TCCATTAATTAATTTCTTCT+TGG	+	chr8_3:4413855-4413874	None:intergenic	20.0%
!!	AAGAAGAAATTAATTAATGG+AGG	-	chr8_3:4413854-4413873	Msa1271030:five_prime_UTR	20.0%
!!!	AAAAGTGTTCAGCTTTTTTA+GGG	-	chr8_3:4411486-4411505	Msa1271030:three_prime_UTR	25.0%
!!!	AAGTCATTCTTTATTTTTGG+AGG	+	chr8_3:4411790-4411809	None:intergenic	25.0%
!	TTCATTTCATTTCATTTCAC+AGG	-	chr8_3:4411841-4411860	Msa1271030:intron	25.0%
!!	TCAATGATTGAGTTGTTAAA+GGG	+	chr8_3:4412637-4412656	None:intergenic	25.0%
!!!	TTTAATTTGCACTTATGGTA+TGG	+	chr8_3:4412735-4412754	None:intergenic	25.0%
!!!	TGTTGTTTAATTTGCACTTA+TGG	+	chr8_3:4412740-4412759	None:intergenic	25.0%
!!	AACTTGTGTTAATTTGAGTT+AGG	-	chr8_3:4412771-4412790	Msa1271030:intron	25.0%
!!!	GTGAGTAGTATATGTTTTAA+GGG	+	chr8_3:4413050-4413069	None:intergenic	25.0%
!!	AGTGAGTAGTATATGTTTTA+AGG	+	chr8_3:4413051-4413070	None:intergenic	25.0%
!	TGTGAATATCTTGAGTATTT+GGG	+	chr8_3:4413395-4413414	None:intergenic	25.0%
!	TTGTGAATATCTTGAGTATT+TGG	+	chr8_3:4413396-4413415	None:intergenic	25.0%
!!!	ATAAGTAACTCCCTTATTTT+TGG	+	chr8_3:4413540-4413559	None:intergenic	25.0%
!	ACTTATCATAAGCATGATTA+TGG	-	chr8_3:4413554-4413573	Msa1271030:five_prime_UTR	25.0%
!	TGAAATTAATATTGGGAGAA+AGG	+	chr8_3:4413942-4413961	None:intergenic	25.0%
!!	TTACTACTTGTTAGTAAGAA+AGG	-	chr8_3:4413992-4414011	Msa1271030:five_prime_UTR	25.0%
	TACAAGAGTAACTTTCCTAT+TGG	-	chr8_3:4411438-4411457	Msa1271030:three_prime_UTR	30.0%
!!!	CAAAAGTGTTCAGCTTTTTT+AGG	-	chr8_3:4411485-4411504	Msa1271030:three_prime_UTR	30.0%
!!	TTGTTTCTGCTATGCTAAAT+GGG	+	chr8_3:4411539-4411558	None:intergenic	30.0%
!!	TTTGTTTCTGCTATGCTAAA+TGG	+	chr8_3:4411540-4411559	None:intergenic	30.0%
	TTTGCAGAAAAGTTATGGTT+GGG	+	chr8_3:4411736-4411755	None:intergenic	30.0%
	ATTTGCAGAAAAGTTATGGT+TGG	+	chr8_3:4411737-4411756	None:intergenic	30.0%
!	CCTAATTTGCAGAAAAGTTA+TGG	+	chr8_3:4411741-4411760	None:intergenic	30.0%
!	CCATAACTTTTCTGCAAATT+AGG	-	chr8_3:4411738-4411757	Msa1271030:CDS	30.0%
!	ACTTTTCTGCAAATTAGGTT+AGG	-	chr8_3:4411743-4411762	Msa1271030:CDS	30.0%
!	CTTTTCTGCAAATTAGGTTA+GGG	-	chr8_3:4411744-4411763	Msa1271030:CDS	30.0%
!	CAATGATTGAGTTGTTAAAG+GGG	+	chr8_3:4412636-4412655	None:intergenic	30.0%
!	CTCAATGATTGAGTTGTTAA+AGG	+	chr8_3:4412638-4412657	None:intergenic	30.0%
!!!	GGTTTTAAAAACGTGTATGA+GGG	-	chr8_3:4412870-4412889	Msa1271030:CDS	30.0%
	AAGGTTGGATTCAAAAAAGA+CGG	-	chr8_3:4412952-4412971	Msa1271030:CDS	30.0%
!!	TACTACTCACTTAGTTTGTT+TGG	-	chr8_3:4413060-4413079	Msa1271030:CDS	30.0%
	TATCAAACTCAGATAGAAGA+TGG	+	chr8_3:4413308-4413327	None:intergenic	30.0%
	CTTCTATCTGAGTTTGATAA+TGG	-	chr8_3:4413309-4413328	Msa1271030:CDS	30.0%
	TCACAAATCTCACAATACTT+TGG	-	chr8_3:4413410-4413429	Msa1271030:CDS	30.0%
	GAAAACAGTGTGTAGTGTAA+TGG	+	chr8_3:4411658-4411677	None:intergenic	35.0%
	TTGCAGAAAAGTTATGGTTG+GGG	+	chr8_3:4411735-4411754	None:intergenic	35.0%
!	TTCTTTCTAGAGAGACATGT+TGG	-	chr8_3:4411878-4411897	Msa1271030:intron	35.0%
	AGACATGTTGGACTATGAAT+GGG	-	chr8_3:4411890-4411909	Msa1271030:intron	35.0%
	TTATCCGACAACTACCTTAA+CGG	-	chr8_3:4412026-4412045	Msa1271030:CDS	35.0%
	AGTGAAACTCACAAACTTTG+TGG	+	chr8_3:4412526-4412545	None:intergenic	35.0%
!!!	GGGTTTTAAAAACGTGTATG+AGG	-	chr8_3:4412869-4412888	Msa1271030:CDS	35.0%
!!!	GCTGTGTTGTTTTGTTTGAA+TGG	+	chr8_3:4413145-4413164	None:intergenic	35.0%
	GACCTTTGTCGAAAATAATC+CGG	+	chr8_3:4413668-4413687	None:intergenic	35.0%
	CCTAGTTAATAGCTACACAT+TGG	+	chr8_3:4413766-4413785	None:intergenic	35.0%
	CCAATGTGTAGCTATTAACT+AGG	-	chr8_3:4413763-4413782	Msa1271030:five_prime_UTR	35.0%
	TGCAGAAAAGTTATGGTTGG+GGG	+	chr8_3:4411734-4411753	None:intergenic	40.0%
	GAGACATGTTGGACTATGAA+TGG	-	chr8_3:4411889-4411908	Msa1271030:intron	40.0%
	GACATGTTGGACTATGAATG+GGG	-	chr8_3:4411891-4411910	Msa1271030:intron	40.0%
	CGGTAAGCATGATGTTTGTT+GGG	+	chr8_3:4411920-4411939	None:intergenic	40.0%
	CCAACAAACATCATGCTTAC+CGG	-	chr8_3:4411918-4411937	Msa1271030:intron	40.0%
	GGAGTTGTTGTCTTCATTTC+CGG	+	chr8_3:4411940-4411959	None:intergenic	40.0%
	CGTAGTGGTCGAAAATTTGA+CGG	+	chr8_3:4411992-4412011	None:intergenic	40.0%
!	CTGTTAACATCCTCGGATTT+TGG	+	chr8_3:4412302-4412321	None:intergenic	40.0%
	CAAAATCCGAGGATGTTAAC+AGG	-	chr8_3:4412300-4412319	Msa1271030:intron	40.0%
	TAGATCAGCATTGCAACCTT+CGG	+	chr8_3:4412477-4412496	None:intergenic	40.0%
	TTGCAATGCTGATCTATCTC+AGG	-	chr8_3:4412481-4412500	Msa1271030:intron	40.0%
	GAAACTCACAAACTTTGTGG+CGG	+	chr8_3:4412523-4412542	None:intergenic	40.0%
	AGTTTGTGAGTTTCACTCCA+AGG	-	chr8_3:4412529-4412548	Msa1271030:intron	40.0%
!	TATTTCTGTGCCTCACTTCA+AGG	-	chr8_3:4412669-4412688	Msa1271030:intron	40.0%
	ATTTCTGTGCCTCACTTCAA+GGG	-	chr8_3:4412670-4412689	Msa1271030:intron	40.0%
	AGTAAGCAAGCACAAAACAG+CGG	-	chr8_3:4412911-4412930	Msa1271030:CDS	40.0%
	AAAAGACGGAAATACCCTTG+AGG	-	chr8_3:4412966-4412985	Msa1271030:CDS	40.0%
	AAAGACGGAAATACCCTTGA+GGG	-	chr8_3:4412967-4412986	Msa1271030:CDS	40.0%
!!	ATGATTGAGTTGACCGTACA+TGG	-	chr8_3:4413001-4413020	Msa1271030:CDS	40.0%
!!	TGATTGAGTTGACCGTACAT+GGG	-	chr8_3:4413002-4413021	Msa1271030:CDS	40.0%
!	ATAATGGAAAACGTAGCTGC+AGG	-	chr8_3:4413325-4413344	Msa1271030:CDS	40.0%
	TGGAACTCAATCTACATCGT+CGG	-	chr8_3:4413622-4413641	Msa1271030:intron	40.0%
	CTTTGTCGAAAATAATCCGG+TGG	+	chr8_3:4413665-4413684	None:intergenic	40.0%
!	CACCGGATTATTTTCGACAA+AGG	-	chr8_3:4413663-4413682	Msa1271030:intron	40.0%
	TTGAGCTTGAAGTCGTTGAA+GGG	+	chr8_3:4413706-4413725	None:intergenic	40.0%
	ATTGAGCTTGAAGTCGTTGA+AGG	+	chr8_3:4413707-4413726	None:intergenic	40.0%
!	AGCTCAATGTTTTTCTCCAC+CGG	-	chr8_3:4413719-4413738	Msa1271030:five_prime_UTR	40.0%
!!	TAATTAATGGAGGAGCGTCA+CGG	-	chr8_3:4413864-4413883	Msa1271030:five_prime_UTR	40.0%
	CCTCATCTCTCTCTACCAAT+AGG	+	chr8_3:4411456-4411475	None:intergenic	45.0%
	CCTATTGGTAGAGAGAGATG+AGG	-	chr8_3:4411453-4411472	Msa1271030:three_prime_UTR	45.0%
	CCGGTAAGCATGATGTTTGT+TGG	+	chr8_3:4411921-4411940	None:intergenic	45.0%
!	AATTTGACGGTGGGCTTGAT+CGG	+	chr8_3:4411979-4411998	None:intergenic	45.0%
	GTGGTCGAAAATTTGACGGT+GGG	+	chr8_3:4411988-4412007	None:intergenic	45.0%
	AGTGGTCGAAAATTTGACGG+TGG	+	chr8_3:4411989-4412008	None:intergenic	45.0%
!	ACATAGCTTGAGAAGCGTAG+TGG	+	chr8_3:4412007-4412026	None:intergenic	45.0%
	AGGTCCGTTAAGGTAGTTGT+CGG	+	chr8_3:4412033-4412052	None:intergenic	45.0%
	ATCACCGCTGTTTATGTCGA+TGG	+	chr8_3:4412069-4412088	None:intergenic	45.0%
!	CGAAGAAGGACTGATGGATA+TGG	+	chr8_3:4412151-4412170	None:intergenic	45.0%
	TATGGGCCTGTTAACATCCT+CGG	+	chr8_3:4412309-4412328	None:intergenic	45.0%
	TCTAGACTTGGACTCAACCT+TGG	-	chr8_3:4412341-4412360	Msa1271030:intron	45.0%
!	CTTCGGAAGAGAAGTATGTG+CGG	+	chr8_3:4412370-4412389	None:intergenic	45.0%
	ACGAAAATGCCCTTGAAGTG+AGG	+	chr8_3:4412682-4412701	None:intergenic	45.0%
	AAGCACAAAACAGCGGTGTT+GGG	-	chr8_3:4412918-4412937	Msa1271030:CDS	45.0%
	AAGACGGAAATACCCTTGAG+GGG	-	chr8_3:4412968-4412987	Msa1271030:CDS	45.0%
	ATTGTCAACTCTCCCCTCAA+GGG	+	chr8_3:4412983-4413002	None:intergenic	45.0%
!!	GATTGAGTTGACCGTACATG+GGG	-	chr8_3:4413003-4413022	Msa1271030:CDS	45.0%
	TTAAGGGACACAAATGCCCT+CGG	+	chr8_3:4413034-4413053	None:intergenic	45.0%
	GTAGATTGAGTTCCAGACTC+TGG	+	chr8_3:4413617-4413636	None:intergenic	45.0%
	TTGAAGTCGTTGAAGGGCTT+GGG	+	chr8_3:4413700-4413719	None:intergenic	45.0%
!	GCTCAATGTTTTTCTCCACC+GGG	-	chr8_3:4413720-4413739	Msa1271030:five_prime_UTR	45.0%
!	TACACATTGGTTGCGTACGT+AGG	+	chr8_3:4413753-4413772	None:intergenic	45.0%
!!!	TATTTTTTTTTTTATCAAAA+AGG	-	chr8_3:4412822-4412841	Msa1271030:intron	5.0%
!!	AAATAATAATAATAATATAG+TGG	+	chr8_3:4413209-4413228	None:intergenic	5.0%
!!	AAATTAAATTAAATTAAACT+GGG	+	chr8_3:4413803-4413822	None:intergenic	5.0%
	GGTAGAGAGAGATGAGGAGA+AGG	-	chr8_3:4411459-4411478	Msa1271030:three_prime_UTR	50.0%
!	GACAACTACCTTAACGGACC+TGG	-	chr8_3:4412032-4412051	Msa1271030:CDS	50.0%
	CCGCTGTTTATGTCGATGGT+GGG	+	chr8_3:4412065-4412084	None:intergenic	50.0%
	CCCACCATCGACATAAACAG+CGG	-	chr8_3:4412062-4412081	Msa1271030:CDS	50.0%
	ACCGCTGTTTATGTCGATGG+TGG	+	chr8_3:4412066-4412085	None:intergenic	50.0%
!!	TAAACTGGTTGTGGTGGTGG+TGG	+	chr8_3:4412109-4412128	None:intergenic	50.0%
!!	CTGATGGATATGGTGCTGCT+GGG	+	chr8_3:4412141-4412160	None:intergenic	50.0%
!!	ACTGATGGATATGGTGCTGC+TGG	+	chr8_3:4412142-4412161	None:intergenic	50.0%
	ATCAGTCCTTCTTCGACCCA+CGG	-	chr8_3:4412156-4412175	Msa1271030:intron	50.0%
!!	AAGCAGTTGATGACCCACCA+TGG	+	chr8_3:4412187-4412206	None:intergenic	50.0%
	ATGGAAGGAGGTTGTGGTTG+AGG	+	chr8_3:4412224-4412243	None:intergenic	50.0%
	ATGACCAGGAAGAGGGTCAA+GGG	+	chr8_3:4412252-4412271	None:intergenic	50.0%
	CATGTCCATGACCAGGAAGA+GGG	+	chr8_3:4412259-4412278	None:intergenic	50.0%
	TTGGTACGAGGAGGAATCCA+GGG	+	chr8_3:4412283-4412302	None:intergenic	50.0%
	TTTGGTACGAGGAGGAATCC+AGG	+	chr8_3:4412284-4412303	None:intergenic	50.0%
!!	CATCCTCGGATTTTGGTACG+AGG	+	chr8_3:4412295-4412314	None:intergenic	50.0%
	TAACAGGCCCATAGACTTCG+TGG	-	chr8_3:4412316-4412335	Msa1271030:intron	50.0%
	AACAGGCCCATAGACTTCGT+GGG	-	chr8_3:4412317-4412336	Msa1271030:intron	50.0%
	CTAGAGCCCACGAAGTCTAT+GGG	+	chr8_3:4412326-4412345	None:intergenic	50.0%
!	TCTAGAGCCCACGAAGTCTA+TGG	+	chr8_3:4412327-4412346	None:intergenic	50.0%
	AGACTTGGACTCAACCTTGG+TGG	-	chr8_3:4412344-4412363	Msa1271030:intron	50.0%
	TTCGAAGAACCTGTCGAACC+CGG	+	chr8_3:4412437-4412456	None:intergenic	50.0%
	ACTCACAAACTTTGTGGCGG+CGG	+	chr8_3:4412520-4412539	None:intergenic	50.0%
	ATCACAACCTGCTGCATTGC+TGG	+	chr8_3:4412595-4412614	None:intergenic	50.0%
	CAAGCACAAAACAGCGGTGT+TGG	-	chr8_3:4412917-4412936	Msa1271030:CDS	50.0%
!	AGCACAAAACAGCGGTGTTG+GGG	-	chr8_3:4412919-4412938	Msa1271030:CDS	50.0%
	CATTGTCAACTCTCCCCTCA+AGG	+	chr8_3:4412984-4413003	None:intergenic	50.0%
	TAAGGGACACAAATGCCCTC+GGG	+	chr8_3:4413033-4413052	None:intergenic	50.0%
	ACGTAGCTGCAGGAAGAGAT+TGG	-	chr8_3:4413335-4413354	Msa1271030:CDS	50.0%
	TGGCTGATCATAATCGTCGC+AGG	-	chr8_3:4413355-4413374	Msa1271030:CDS	50.0%
	ATTGAGTTCCAGACTCTGGC+GGG	+	chr8_3:4413613-4413632	None:intergenic	50.0%
	GATTGAGTTCCAGACTCTGG+CGG	+	chr8_3:4413614-4413633	None:intergenic	50.0%
	ATCTACATCGTCGGTGACAG+TGG	-	chr8_3:4413631-4413650	Msa1271030:intron	50.0%
	CTTGAAGTCGTTGAAGGGCT+TGG	+	chr8_3:4413701-4413720	None:intergenic	50.0%
!	TACCTTAACGGACCTGGTGC+TGG	-	chr8_3:4412038-4412057	Msa1271030:CDS	55.0%
	GGCTAAACTGGTTGTGGTGG+TGG	+	chr8_3:4412112-4412131	None:intergenic	55.0%
!	GTGGGCTAAACTGGTTGTGG+TGG	+	chr8_3:4412115-4412134	None:intergenic	55.0%
!	CCTGTGGGCTAAACTGGTTG+TGG	+	chr8_3:4412118-4412137	None:intergenic	55.0%
	CCACAACCAGTTTAGCCCAC+AGG	-	chr8_3:4412115-4412134	Msa1271030:CDS	55.0%
	GTGGGTCGAAGAAGGACTGA+TGG	+	chr8_3:4412157-4412176	None:intergenic	55.0%
	TCAGTCCTTCTTCGACCCAC+GGG	-	chr8_3:4412157-4412176	Msa1271030:intron	55.0%
	GAGGTTGTGGTTGAGGTGGA+GGG	+	chr8_3:4412217-4412236	None:intergenic	55.0%
	GAAGGAGGTTGTGGTTGAGG+TGG	+	chr8_3:4412221-4412240	None:intergenic	55.0%
!	GAGAGCATGGAAGGAGGTTG+TGG	+	chr8_3:4412230-4412249	None:intergenic	55.0%
!	TCAAGGGAGAGCATGGAAGG+AGG	+	chr8_3:4412236-4412255	None:intergenic	55.0%
	AAGAGGGTCAAGGGAGAGCA+TGG	+	chr8_3:4412243-4412262	None:intergenic	55.0%
	CATGACCAGGAAGAGGGTCA+AGG	+	chr8_3:4412253-4412272	None:intergenic	55.0%
	CTTGACCCTCTTCCTGGTCA+TGG	-	chr8_3:4412251-4412270	Msa1271030:intron	55.0%
	CCATGTCCATGACCAGGAAG+AGG	+	chr8_3:4412260-4412279	None:intergenic	55.0%
	CCTCTTCCTGGTCATGGACA+TGG	-	chr8_3:4412257-4412276	Msa1271030:intron	55.0%
!!	CCTCGGATTTTGGTACGAGG+AGG	+	chr8_3:4412292-4412311	None:intergenic	55.0%
	CCTCCTCGTACCAAAATCCG+AGG	-	chr8_3:4412289-4412308	Msa1271030:intron	55.0%
!!	GACTTCGTGGGCTCTAGACT+TGG	-	chr8_3:4412329-4412348	Msa1271030:intron	55.0%
!	AGAAGTATGTGCGGCCACCA+AGG	+	chr8_3:4412361-4412380	None:intergenic	55.0%
	GTCTAGACCGACGGTAAAGG+CGG	+	chr8_3:4412406-4412425	None:intergenic	55.0%
	GTGGTCTAGACCGACGGTAA+AGG	+	chr8_3:4412409-4412428	None:intergenic	55.0%
	GTTCAGGTGGTCTAGACCGA+CGG	+	chr8_3:4412415-4412434	None:intergenic	55.0%
	TCGGTCTAGACCACCTGAAC+CGG	-	chr8_3:4412415-4412434	Msa1271030:intron	55.0%
	CAGGTTCTTCGAACTCGCCT+CGG	-	chr8_3:4412444-4412463	Msa1271030:intron	55.0%
	AGTCAACCCAGCTGCAACGA+CGG	+	chr8_3:4412561-4412580	None:intergenic	55.0%
!	TTGCAGCTGGGTTGACTCAG+CGG	-	chr8_3:4412564-4412583	Msa1271030:intron	55.0%
!	GCACAAAACAGCGGTGTTGG+GGG	-	chr8_3:4412920-4412939	Msa1271030:CDS	55.0%
!	AAACAGCGGTGTTGGGGGAT+AGG	-	chr8_3:4412925-4412944	Msa1271030:CDS	55.0%
!	AACAGCGGTGTTGGGGGATA+GGG	-	chr8_3:4412926-4412945	Msa1271030:CDS	55.0%
	CCTCGGGATTTCCCCATGTA+CGG	+	chr8_3:4413017-4413036	None:intergenic	55.0%
	CGTACATGGGGAAATCCCGA+GGG	-	chr8_3:4413015-4413034	Msa1271030:CDS	55.0%
	TTGAGTTCCAGACTCTGGCG+GGG	+	chr8_3:4413612-4413631	None:intergenic	55.0%
	TACATCGTCGGTGACAGTGG+CGG	-	chr8_3:4413634-4413653	Msa1271030:intron	55.0%
	TCGTTGAAGGGCTTGGGCTT+TGG	+	chr8_3:4413694-4413713	None:intergenic	55.0%
	TACGTAGGTTCACTACCCGG+TGG	+	chr8_3:4413738-4413757	None:intergenic	55.0%
	GCGTACGTAGGTTCACTACC+CGG	+	chr8_3:4413741-4413760	None:intergenic	55.0%
	TTGATCGGAGGCTGCTGCTG+CGG	+	chr8_3:4411964-4411983	None:intergenic	60.0%
!	TTGACGGTGGGCTTGATCGG+AGG	+	chr8_3:4411976-4411995	None:intergenic	60.0%
	GTCCAGCACCAGGTCCGTTA+AGG	+	chr8_3:4412043-4412062	None:intergenic	60.0%
!!	TATGGTGCTGCTGGGCCTGT+GGG	+	chr8_3:4412133-4412152	None:intergenic	60.0%
!!	ATATGGTGCTGCTGGGCCTG+TGG	+	chr8_3:4412134-4412153	None:intergenic	60.0%
	GAAAGCCCGTGGGTCGAAGA+AGG	+	chr8_3:4412165-4412184	None:intergenic	60.0%
	TTCGACCCACGGGCTTTCCA+TGG	-	chr8_3:4412167-4412186	Msa1271030:intron	60.0%
	ACCCACGGGCTTTCCATGGT+GGG	-	chr8_3:4412171-4412190	Msa1271030:intron	60.0%
	ACCCACCATGGAAAGCCCGT+GGG	+	chr8_3:4412175-4412194	None:intergenic	60.0%
	GGAGGTTGTGGTTGAGGTGG+AGG	+	chr8_3:4412218-4412237	None:intergenic	60.0%
!	GGGTCAAGGGAGAGCATGGA+AGG	+	chr8_3:4412239-4412258	None:intergenic	60.0%
	CTCTCCCTTGACCCTCTTCC+TGG	-	chr8_3:4412245-4412264	Msa1271030:intron	60.0%
	GCGGCTCACGAAGTCGTCTT+CGG	+	chr8_3:4412387-4412406	None:intergenic	60.0%
	TCGTGAGCCGCCTTTACCGT+CGG	-	chr8_3:4412396-4412415	Msa1271030:intron	60.0%
	CGGTCTAGACCACCTGAACC+GGG	-	chr8_3:4412416-4412435	Msa1271030:intron	60.0%
	GAACCTGTCGAACCCGGTTC+AGG	+	chr8_3:4412431-4412450	None:intergenic	60.0%
!!	TTCGAACTCGCCTCGGTGTC+AGG	-	chr8_3:4412451-4412470	Msa1271030:intron	60.0%
	CACAAACTTTGTGGCGGCGG+TGG	+	chr8_3:4412517-4412536	None:intergenic	60.0%
	GGTTCTGCCAGCAATGCAGC+AGG	-	chr8_3:4412585-4412604	Msa1271030:intron	60.0%
!	ACAGCGGTGTTGGGGGATAG+GGG	-	chr8_3:4412927-4412946	Msa1271030:CDS	60.0%
	GTGTTGGGGGATAGGGGAGA+AGG	-	chr8_3:4412933-4412952	Msa1271030:CDS	60.0%
	TGGGGGATAGGGGAGAAGGT+TGG	-	chr8_3:4412937-4412956	Msa1271030:CDS	60.0%
	CCGTACATGGGGAAATCCCG+AGG	-	chr8_3:4413014-4413033	Msa1271030:CDS	60.0%
	GACAGTGGCGGTTTCTCCAC+CGG	-	chr8_3:4413646-4413665	Msa1271030:intron	60.0%
!!	TCGATGGTGGGTCCAGCACC+AGG	+	chr8_3:4412053-4412072	None:intergenic	65.0%
!	GCTGGGCCTGTGGGCTAAAC+TGG	+	chr8_3:4412124-4412143	None:intergenic	65.0%
	GACCCACGGGCTTTCCATGG+TGG	-	chr8_3:4412170-4412189	Msa1271030:intron	65.0%
	GACCCACCATGGAAAGCCCG+TGG	+	chr8_3:4412176-4412195	None:intergenic	65.0%
	CCAGGGCCATGTCCATGACC+AGG	+	chr8_3:4412266-4412285	None:intergenic	65.0%
	CCTGGTCATGGACATGGCCC+TGG	-	chr8_3:4412263-4412282	Msa1271030:intron	65.0%
	CCTGTCGAACCCGGTTCAGG+TGG	+	chr8_3:4412428-4412447	None:intergenic	65.0%
	CCACCTGAACCGGGTTCGAC+AGG	-	chr8_3:4412425-4412444	Msa1271030:intron	65.0%
	CAACCTTCGGCCTGACACCG+AGG	+	chr8_3:4412464-4412483	None:intergenic	65.0%
	TGCAACGACGGTGGCTGCCT+TGG	+	chr8_3:4412549-4412568	None:intergenic	65.0%
	CAGCCACCGTCGTTGCAGCT+GGG	-	chr8_3:4412552-4412571	Msa1271030:intron	65.0%
	CAACCCAGCTGCAACGACGG+TGG	+	chr8_3:4412558-4412577	None:intergenic	65.0%
	GAAGGATCCCCGCCAGAGTC+TGG	-	chr8_3:4413602-4413621	Msa1271030:intron	65.0%
	ATCGGAGGCTGCTGCTGCGG+CGG	+	chr8_3:4411961-4411980	None:intergenic	70.0%
!!	TCGCCTCGGTGTCAGGCCGA+AGG	-	chr8_3:4412458-4412477	Msa1271030:intron	70.0%
	GCAGCCACCGTCGTTGCAGC+TGG	-	chr8_3:4412551-4412570	Msa1271030:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	4411400	4414039	4411400	ID=Msa1271030;Name=Msa1271030
chr8_3	mRNA	4411400	4414039	4411400	ID=Msa1271030-mRNA-1;Parent=Msa1271030;Name=Msa1271030-mRNA-1;_AED=0.10;_eAED=0.10;_QI=391\|1\|1\|1\|0.66\|0.5\|4\|294\|332
chr8_3	exon	4411400	4411833	4411400	ID=Msa1271030-mRNA-1:exon:1561;Parent=Msa1271030-mRNA-1
chr8_3	exon	4411992	4412137	4411992	ID=Msa1271030-mRNA-1:exon:1560;Parent=Msa1271030-mRNA-1
chr8_3	exon	4412833	4413576	4412833	ID=Msa1271030-mRNA-1:exon:1559;Parent=Msa1271030-mRNA-1
chr8_3	exon	4413680	4414039	4413680	ID=Msa1271030-mRNA-1:exon:1558;Parent=Msa1271030-mRNA-1
chr8_3	five_prime_UTR	4413680	4414039	4413680	ID=Msa1271030-mRNA-1:five_prime_utr;Parent=Msa1271030-mRNA-1
chr8_3	five_prime_UTR	4413546	4413576	4413546	ID=Msa1271030-mRNA-1:five_prime_utr;Parent=Msa1271030-mRNA-1
chr8_3	CDS	4412833	4413545	4412833	ID=Msa1271030-mRNA-1:cds;Parent=Msa1271030-mRNA-1
chr8_3	CDS	4411992	4412137	4411992	ID=Msa1271030-mRNA-1:cds;Parent=Msa1271030-mRNA-1
chr8_3	CDS	4411694	4411833	4411694	ID=Msa1271030-mRNA-1:cds;Parent=Msa1271030-mRNA-1
chr8_3	three_prime_UTR	4411400	4411693	4411400	ID=Msa1271030-mRNA-1:three_prime_utr;Parent=Msa1271030-mRNA-1

Gene Sequence

>Msa1271030

ATGTTGGACTATGAATGGGGTAACCCAACAAACATCATGCTTACCGGAAATGAAGACAACAACTCCGCCGCAGCAGCAGCCTCCGATCAAGCCCACCGTCAAATTTTCGACCACTACGCTTCTCAAGCTATGTTATCCGACAACTACCTTAACGGACCTGGTGCTGGACCCACCATCGACATAAACAGCGGTGATTTTACTCATCATAATCACCACCACCACCACAACCAGTTTAGCCCACAGGCCCAGCAGCACCATATCCATCAGTCCTTCTTCGACCCACGGGCTTTCCATGGTGGGTCATCAACTGCTTCTTCTTACCCTCCACCTCAACCACAACCTCCTTCCATGCTCTCCCTTGACCCTCTTCCTGGTCATGGACATGGCCCTGGATTCCTCCTCGTACCAAAATCCGAGGATGTTAACAGGCCCATAGACTTCGTGGGCTCTAGACTTGGACTCAACCTTGGTGGCCGCACATACTTCTCTTCCGAAGACGACTTCGTGAGCCGCCTTTACCGTCGGTCTAGACCACCTGAACCGGGTTCGACAGGTTCTTCGAACTCGCCTCGGTGTCAGGCCGAAGGTTGCAATGCTGATCTATCTCAGGCGAAGCACTACCACCGCCGCCACAAAGTTTGTGAGTTTCACTCCAAGGCAGCCACCGTCGTTGCAGCTGGGTTGACTCAGCGGTTCTGCCAGCAATGCAGCAGGTTCCATCTTCTATCTGAGTTTGATAATGGAAAACGTAGCTGCAGGAAGAGATTGGCTGATCATAATCGTCGCAGGAGAAAAACTCAGCACCCAAATACTCAAGATATTCACAAATCTCACAATACTTTGGATAATTCTTCCACAAGATCCCCGCCAGAGTCTGGAACTCAATCTACATCGTCGGTGACAGTGGCGGTTTCTCCACCGGATTATTTTCGACAAAGGTCGTACCAAAGCCCAAGCCCTTCAACGACTTCAAGCTCAATGTTTTTCTCCACCGGGTAG

Protein sequence

>Msa1271030

MLDYEWGNPTNIMLTGNEDNNSAAAAASDQAHRQIFDHYASQAMLSDNYLNGPGAGPTIDINSGDFTHHNHHHHHNQFSPQAQQHHIHQSFFDPRAFHGGSSTASSYPPPQPQPPSMLSLDPLPGHGHGPGFLLVPKSEDVNRPIDFVGSRLGLNLGGRTYFSSEDDFVSRLYRRSRPPEPGSTGSSNSPRCQAEGCNADLSQAKHYHRRHKVCEFHSKAATVVAAGLTQRFCQQCSRFHLLSEFDNGKRSCRKRLADHNRRRRKTQHPNTQDIHKSHNTLDNSSTRSPPESGTQSTSSVTVAVSPPDYFRQRSYQSPSPSTTSSSMFFSTG*