AlfalfaGEDB | Msa1308130

M. sativa cultivar zhongmu-4 / Msa1308130

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007049 GO:0007275 GO:0008150 GO:0009553 GO:0009554 GO:0009555 GO:0009556 GO:0009791 GO:0009908 GO:0009987 GO:0019953 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0034293 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043934 GO:0044424 GO:0044464 GO:0044703 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0051321 GO:0051704 GO:0061458 GO:0090567 GO:0097159 GO:0099402 GO:1901363 GO:1903046
PFAM: PF03110.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1308130	sp\|Q8GXL3\|SPL8_ARATH	44.944	356	115	12	1	319	1	312	4.70e-80	250

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1308130	G7LBJ7	91.900	321	14	5	1	319	1	311	0.0	569

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TFs/TRs:

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Gene ID	Type	Classification
Msa1308130	TF	SBP

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0044100	Msa1308130	0.816035	6.804436e-52	-8.615850e-47
Msa0141100	Msa1308130	0.803205	3.895477e-49	-8.615850e-47
Msa0183750	Msa1308130	0.821056	4.946504e-53	-8.615850e-47
Msa0326540	Msa1308130	0.805012	1.639312e-49	-8.615850e-47
Msa0374850	Msa1308130	0.804600	1.998836e-49	-8.615850e-47
Msa0385670	Msa1308130	0.877545	5.769328e-69	-8.615850e-47
Msa0418000	Msa1308130	0.803007	4.281490e-49	-8.615850e-47
Msa0435670	Msa1308130	0.872444	3.165475e-67	-8.615850e-47
Msa0470860	Msa1308130	0.841231	5.387733e-58	-8.615850e-47
Msa0479680	Msa1308130	0.872932	2.174246e-67	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1308130	MtrunA17_Chr8g0334671	91.900	321	14	5	1	319	1	311	0.0	569
Msa1308130	MtrunA17_Chr8g0360361	70.000	90	26	1	193	282	63	151	1.13e-35	127
Msa1308130	MtrunA17_Chr4g0046991	72.619	84	22	1	187	269	64	147	2.37e-35	125
Msa1308130	MtrunA17_Chr1g0194121	48.649	148	63	3	122	269	87	221	4.20e-35	135
Msa1308130	MtrunA17_Chr2g0302791	52.419	124	53	2	155	278	104	221	8.37e-35	134
Msa1308130	MtrunA17_Chr7g0231911	67.857	84	27	0	188	271	142	225	1.31e-34	128
Msa1308130	MtrunA17_Chr3g0122411	54.472	123	49	2	155	270	118	240	6.89e-34	129
Msa1308130	MtrunA17_Chr2g0282521	67.470	83	27	0	187	269	56	138	2.37e-33	120
Msa1308130	MtrunA17_Chr4g0061651	70.370	81	24	0	190	270	118	198	2.76e-33	128
Msa1308130	MtrunA17_Chr2g0308321	72.368	76	21	0	195	270	171	246	3.40e-33	129

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1308130	AT1G02065.1	44.944	356	115	12	1	319	1	312	4.78e-81	250
Msa1308130	AT1G02065.2	44.118	272	85	8	1	242	1	235	1.10e-59	192
Msa1308130	AT1G69170.1	53.543	127	52	3	142	268	78	197	2.26e-34	130
Msa1308130	AT1G27370.6	47.368	152	59	3	135	270	105	251	3.65e-34	128
Msa1308130	AT1G20980.1	57.627	118	49	1	155	272	81	197	3.87e-34	133
Msa1308130	AT1G27370.1	47.368	152	59	3	135	270	105	251	7.16e-34	129
Msa1308130	AT1G27370.5	47.368	152	59	3	135	270	105	251	7.16e-34	129
Msa1308130	AT1G27370.3	47.368	152	59	3	135	270	105	251	7.16e-34	129
Msa1308130	AT1G27370.2	47.368	152	59	3	135	270	105	251	7.16e-34	129
Msa1308130	AT1G27370.4	47.368	152	59	3	135	270	105	251	7.16e-34	129

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Find 158 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTAAATTAAATTAAACT+GGG	0.605549	8_4:+4289282	None:intergenic
AACAGGCCCATAGACTTCGT+GGG	0.651739	8_4:-4290780	Msa1308130:CDS
AACCAGTTGATGACCCACCA+TGG	0.633020	8_4:+4290919	None:intergenic
AAGAGGGTCAAGGGAGAGCA+TGG	0.508675	8_4:+4290863	None:intergenic
AAGATGAAATTAATTAATGG+AGG	0.566025	8_4:-4289228	Msa1308130:three_prime_UTR
AATTTGACGGTGGGCTTGAT+CGG	0.400326	8_4:+4291130	None:intergenic
ACATAGCTTGAGAAGCGTAG+TGG	0.674169	8_4:+4291102	None:intergenic
ACCACTGTTTATGTCGATGG+TGG	0.584036	8_4:+4291043	None:intergenic
ACCCACCATGGAAAGCCCGT+GGG	0.668555	8_4:+4290931	None:intergenic
ACCCACGGGCTTTCCATGGT+GGG	0.498666	8_4:-4290932	Msa1308130:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCTTTCTGCTATGCTAAAT+GGG	+	chr8_4:4289198-4289217	None:intergenic	30.0%
TTTCTTTCTGCTATGCTAAA+TGG	+	chr8_4:4289199-4289218	None:intergenic	30.0%
GAAAACAGTGTGTAATGTAA+TGG	+	chr8_4:4289304-4289323	None:intergenic	30.0%
TTCTTTATAGAGAGACATGT+TGG	-	chr8_4:4289530-4289549	Msa1308130:intron	30.0%
AAGGTTGGATTCAAAAAAGA+CGG	-	chr8_4:4290610-4290629	Msa1308130:CDS	30.0%
TATCAAACTCAGATAGAAGA+TGG	+	chr8_4:4290984-4291003	None:intergenic	30.0%
CTTCTATCTGAGTTTGATAA+TGG	-	chr8_4:4290985-4291004	Msa1308130:CDS	30.0%
TCACAAATCTCACAATACTT+TGG	-	chr8_4:4291086-4291105	Msa1308130:CDS	30.0%
AGAGAGAGTAACTTTCCTAT+TGG	-	chr8_4:4289095-4289114	Msa1308130:three_prime_UTR	35.0%
TTTGCAGGAAAGTTATGGTT+GGG	+	chr8_4:4289374-4289393	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	4289065	4291715	4289065	ID=Msa1308130;Name=Msa1308130
chr8_4	mRNA	4289065	4291715	4289065	ID=Msa1308130-mRNA-1;Parent=Msa1308130;Name=Msa1308130-mRNA-1;_AED=0.11;_eAED=0.11;_QI=368\|1\|1\|1\|0.66\|0.5\|4\|294\|335
chr8_4	exon	4289065	4289498	4289065	ID=Msa1308130-mRNA-1:exon:1328;Parent=Msa1308130-mRNA-1
chr8_4	exon	4289657	4289802	4289657	ID=Msa1308130-mRNA-1:exon:1327;Parent=Msa1308130-mRNA-1
chr8_4	exon	4290513	4291269	4290513	ID=Msa1308130-mRNA-1:exon:1326;Parent=Msa1308130-mRNA-1
chr8_4	exon	4291383	4291715	4291383	ID=Msa1308130-mRNA-1:exon:1325;Parent=Msa1308130-mRNA-1
chr8_4	five_prime_UTR	4291383	4291715	4291383	ID=Msa1308130-mRNA-1:five_prime_utr;Parent=Msa1308130-mRNA-1
chr8_4	five_prime_UTR	4291235	4291269	4291235	ID=Msa1308130-mRNA-1:five_prime_utr;Parent=Msa1308130-mRNA-1
chr8_4	CDS	4290513	4291234	4290513	ID=Msa1308130-mRNA-1:cds;Parent=Msa1308130-mRNA-1
chr8_4	CDS	4289657	4289802	4289657	ID=Msa1308130-mRNA-1:cds;Parent=Msa1308130-mRNA-1
chr8_4	CDS	4289359	4289498	4289359	ID=Msa1308130-mRNA-1:cds;Parent=Msa1308130-mRNA-1
chr8_4	three_prime_UTR	4289065	4289358	4289065	ID=Msa1308130-mRNA-1:three_prime_utr;Parent=Msa1308130-mRNA-1

Gene Sequence

>Msa1308130

ATGTTGGACTATGAATGGGGTAACCCAACAAACATCATGCTTACCGGAAATGAAGACAACAACTCCGCCGCAGCAGCAGCCTCCGATCAAGCCCACCGTCAAATTTTCGACCACTACGCTTCTCAAGCTATGTTATCCGACAACTACCTTAACGGACCTGGTTCTGGACCCACCATCGACATAAACAGTGGTGATTTTACTCATCATAATAACCACCACCACCACCAGAACCAGTTTAGCCAACAGGCCCAGCAGCACCATATCCATCAGTCCTTCTTCGACCCACGGGCTTTCCATGGTGGGTCATCAACTGGTTCTTCTTACCCTCCACCTCAACCACAACCTCCTTCCATGCTCTCCCTTGACCCTCTTCCTGGTCATGGACATGGACATGGCCCTGGATTCCTCCTCGTACCAAAATCCGAGGATGTTAACAGGCCCATAGACTTCGTGGGCTCTAGACTTGGACTCAACCTTGGTGGCCGCACATACTTCTCTTCCGAAGACGACTTCGTGAGCCGCCTTTACCGTCGGTCTAGACCACCTGAACCGGGTTCGACAGGTTCTTCGAACTCGCCTCGGTGTCAGGCCGAAGGTTGCAATGCTGATCTATCTCAGGCGAAGCACTACCACCGCCGCCACAAAGTTTGTGAGTTTCACTCCAAGGCAGCCACCGTCGTTGCAGCTGGGTTGACTCAGCGGTTCTGCCAGCAATGCAGCAGGTTCCATCTTCTATCTGAGTTTGATAATGGAAAACGTAGCTGCAGGAAGAGATTGGCTGATCATAATCGTCGCAGGAGAAAAACTCAGCACCCAAATACTCAAGATATTCACAAATCTCACAATACTTTGGATAATTCTTCCACAAGATCCCCGCCAGAGTCTGGAACTCAATCTACATCGTCGGTGACAGTGGCGGTTTCTCCACCGGATTATTTTCGACAAAGGTCGTACCAAAGCCCAAGCCCTTCAACAACTTCAAGCTCAATGTTTTTCTCCACCGGGTAG

Protein sequence

>Msa1308130

MLDYEWGNPTNIMLTGNEDNNSAAAAASDQAHRQIFDHYASQAMLSDNYLNGPGSGPTIDINSGDFTHHNNHHHHQNQFSQQAQQHHIHQSFFDPRAFHGGSSTGSSYPPPQPQPPSMLSLDPLPGHGHGHGPGFLLVPKSEDVNRPIDFVGSRLGLNLGGRTYFSSEDDFVSRLYRRSRPPEPGSTGSSNSPRCQAEGCNADLSQAKHYHRRHKVCEFHSKAATVVAAGLTQRFCQQCSRFHLLSEFDNGKRSCRKRLADHNRRRRKTQHPNTQDIHKSHNTLDNSSTRSPPESGTQSTSSVTVAVSPPDYFRQRSYQSPSPSTTSSSMFFSTG*