AlfalfaGEDB | Msa1452060

M. sativa cultivar zhongmu-4 / Msa1452060

KEGG: K15040
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0016020 GO:0019867 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805
PFAM: PF01459.25

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1452060	sp\|Q9SRH5\|VDAC1_ARATH	35.689	283	136	6	24	267	1	276	2.57e-49	167

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1452060	G7JPX4	97.131	244	7	0	24	267	1	244	8.24e-176	493

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0044100	Msa1452060	0.833349	5.612154e-56	-8.615850e-47
Msa1369700	Msa1452060	0.867059	1.809248e-65	-8.615850e-47
Msa1381140	Msa1452060	0.813645	2.304658e-51	-8.615850e-47
Msa1395870	Msa1452060	0.845329	4.346112e-59	-8.615850e-47
Msa1399560	Msa1452060	0.862017	6.822602e-64	-8.615850e-47
Msa1437190	Msa1452060	0.856067	4.132367e-62	-8.615850e-47
Msa1443840	Msa1452060	0.848052	7.835840e-60	-8.615850e-47
Msa1452060	Msa1460380	0.866147	3.527173e-65	-8.615850e-47
Msa0611380	Msa1452060	0.855554	5.836429e-62	-8.615850e-47
Msa0618180	Msa1452060	0.838864	2.232428e-57	-8.615850e-47
Msa0618220	Msa1452060	0.827358	1.634754e-54	-8.615850e-47
Msa0619990	Msa1452060	0.826433	2.720490e-54	-8.615850e-47
Msa0628640	Msa1452060	0.868234	7.596957e-66	-8.615850e-47
Msa0631120	Msa1452060	0.846940	1.583441e-59	-8.615850e-47
Msa0666030	Msa1452060	0.841200	5.487339e-58	-8.615850e-47
Msa0669340	Msa1452060	0.853003	3.184331e-61	-8.615850e-47
Msa0701970	Msa1452060	0.853272	2.666234e-61	-8.615850e-47
Msa0716450	Msa1452060	0.856498	3.089918e-62	-8.615850e-47
Msa0717260	Msa1452060	0.882690	8.433226e-71	-8.615850e-47
Msa0734230	Msa1452060	0.860053	2.700224e-63	-8.615850e-47
Msa0758320	Msa1452060	0.874358	7.189064e-68	-8.615850e-47
Msa0775440	Msa1452060	0.851324	9.559591e-61	-8.615850e-47
Msa0799030	Msa1452060	0.857169	1.960514e-62	-8.615850e-47
Msa0799700	Msa1452060	0.828614	8.152892e-55	-8.615850e-47
Msa0812820	Msa1452060	0.847550	1.077396e-59	-8.615850e-47
Msa0837440	Msa1452060	0.837415	5.269457e-57	-8.615850e-47
Msa0837980	Msa1452060	0.881081	3.228706e-70	-8.615850e-47
Msa0141100	Msa1452060	0.849252	3.643196e-60	-8.615850e-47
Msa0183750	Msa1452060	0.850724	1.411001e-60	-8.615850e-47
Msa0185380	Msa1452060	0.817060	4.011282e-52	-8.615850e-47
Msa0191040	Msa1452060	0.834793	2.440748e-56	-8.615850e-47
Msa0220300	Msa1452060	0.833221	6.038810e-56	-8.615850e-47
Msa0233340	Msa1452060	0.834559	2.795103e-56	-8.615850e-47
Msa1227950	Msa1452060	0.843500	1.348544e-58	-8.615850e-47
Msa1227970	Msa1452060	0.832429	9.499204e-56	-8.615850e-47
Msa1255280	Msa1452060	0.825308	5.032048e-54	-8.615850e-47
Msa1258050	Msa1452060	0.832230	1.064209e-55	-8.615850e-47
Msa1266960	Msa1452060	0.868351	6.965202e-66	-8.615850e-47
Msa1271030	Msa1452060	0.804588	2.009633e-49	-8.615850e-47
Msa1308130	Msa1452060	0.856991	2.212145e-62	-8.615850e-47
Msa1321000	Msa1452060	0.804726	1.881403e-49	-8.615850e-47
Msa1339940	Msa1452060	0.995251	1.932549e-214	-8.615850e-47
Msa0374850	Msa1452060	0.842607	2.332245e-58	-8.615850e-47
Msa0375090	Msa1452060	0.849723	2.692973e-60	-8.615850e-47
Msa0385670	Msa1452060	0.840355	9.138848e-58	-8.615850e-47
Msa0418000	Msa1452060	0.802345	5.861834e-49	-8.615850e-47
Msa0435670	Msa1452060	0.823465	1.365331e-53	-8.615850e-47
Msa0470340	Msa1452060	0.860353	2.191052e-63	-8.615850e-47
Msa0470860	Msa1452060	0.851390	9.154144e-61	-8.615850e-47
Msa0479680	Msa1452060	0.840936	6.438361e-58	-8.615850e-47
Msa0479700	Msa1452060	0.840857	6.753088e-58	-8.615850e-47
Msa0480950	Msa1452060	0.861636	8.923481e-64	-8.615850e-47
Msa0981010	Msa1452060	0.822131	2.791544e-53	-8.615850e-47
Msa1001770	Msa1452060	0.815115	1.090463e-51	-8.615850e-47
Msa1002620	Msa1452060	0.853761	1.930049e-61	-8.615850e-47
Msa1033560	Msa1452060	0.817213	3.705719e-52	-8.615850e-47
Msa0251240	Msa1452060	0.805326	1.409247e-49	-8.615850e-47
Msa0288190	Msa1452060	0.805850	1.093723e-49	-8.615850e-47
Msa0311690	Msa1452060	0.825830	3.785218e-54	-8.615850e-47
Msa0326540	Msa1452060	0.811577	6.531677e-51	-8.615850e-47
Msa1113850	Msa1452060	0.835448	1.668384e-56	-8.615850e-47
Msa1160400	Msa1452060	0.814667	1.370756e-51	-8.615850e-47
Msa1190120	Msa1452060	0.867110	1.742271e-65	-8.615850e-47
Msa1203000	Msa1452060	0.847047	1.480423e-59	-8.615850e-47
Msa1207870	Msa1452060	0.828648	7.998019e-55	-8.615850e-47
Msa1211550	Msa1452060	0.969098	1.260654e-129	-8.615850e-47
Msa1217940	Msa1452060	0.807780	4.275585e-50	-8.615850e-47
Msa1219260	Msa1452060	0.859120	5.152454e-63	-8.615850e-47
Msa0531430	Msa1452060	0.867761	1.078089e-65	-8.615850e-47
Msa0534570	Msa1452060	0.833243	5.965173e-56	-8.615850e-47
Msa0534590	Msa1452060	0.849013	4.244950e-60	-8.615850e-47
Msa0565600	Msa1452060	0.864843	9.082527e-65	-8.615850e-47
Msa0590800	Msa1452060	0.840717	7.347694e-58	-8.615850e-47
Msa0853320	Msa1452060	0.842552	2.411829e-58	-8.615850e-47
Msa0966340	Msa1452060	0.804757	1.853655e-49	-8.615850e-47
Msa0966440	Msa1452060	0.803290	3.741494e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1452060	MtrunA17_Chr4g0060181	97.131	244	7	0	24	267	1	244	1.59e-179	493
Msa1452060	MtrunA17_Chr8g0362621	34.982	283	138	5	24	267	1	276	9.38e-49	162
Msa1452060	MtrunA17_Chr7g0216561	35.106	282	137	6	25	267	3	277	2.14e-47	159
Msa1452060	MtrunA17_Chr6g0452811	34.035	285	140	6	24	267	1	278	2.62e-46	156
Msa1452060	MtrunA17_Chr5g0401201	26.855	283	161	5	24	267	1	276	1.82e-29	112
Msa1452060	MtrunA17_Chr1g0152591	25.177	282	165	5	25	267	3	277	4.87e-24	97.8
Msa1452060	MtrunA17_Chr4g0072511	24.742	291	160	5	24	267	1	279	5.16e-22	92.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1452060	AT3G01280.1	35.689	283	136	6	24	267	1	276	2.61e-50	167
Msa1452060	AT5G15090.1	31.802	283	145	7	24	267	1	274	8.16e-41	142
Msa1452060	AT5G15090.2	31.802	283	145	7	24	267	1	274	8.16e-41	142
Msa1452060	AT5G67500.3	28.421	285	154	6	24	267	1	276	1.93e-31	117
Msa1452060	AT5G57490.1	27.682	289	149	7	24	267	1	274	1.86e-29	112
Msa1452060	AT5G67500.2	26.774	310	154	6	24	267	1	303	1.08e-27	108
Msa1452060	AT5G67500.1	27.509	269	146	5	24	251	1	261	6.69e-27	106
Msa1452060	AT3G49920.1	28.279	244	157	3	24	267	1	226	1.47e-18	82.4

Find 101 sgRNAs with CRISPR-Local

Find 324 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAATGTTTGCTTGCATTTC+AGG	0.110539	tig0033659:+25117	None:intergenic
ATGGCCGGCGAAATGGATTT+TGG	0.152016	tig0033659:-25516	Msa1452060:CDS
ACTGGATGCATTGGATTATT+AGG	0.191506	tig0033659:-26106	Msa1452060:CDS
CTCTCATTCAACAAGGATTT+TGG	0.192793	tig0033659:-25557	Msa1452060:CDS
CGAGCCTTTAGCAATGTTTC+AGG	0.209136	tig0033659:+25606	None:intergenic
CAATAGCTACAGTTGATATT+TGG	0.215196	tig0033659:-27460	Msa1452060:five_prime_UTR
TTTCAAACAATGACACAAAT+TGG	0.223326	tig0033659:-25381	Msa1452060:three_prime_UTR
TTGTTAACATGATAATTTAT+TGG	0.230813	tig0033659:+27353	None:intergenic
TTGCAATGGCCGTCTGGGTT+AGG	0.244222	tig0033659:+25697	None:intergenic
AATAGATTCACTGGGATTAC+TGG	0.244615	tig0033659:-26124	Msa1452060:CDS
ATAAATCTTCTCTCCCCTTT+AGG	0.264493	tig0033659:+25160	None:intergenic
CTTCACTTTCGACATTGTTT+AGG	0.305302	tig0033659:-25189	Msa1452060:three_prime_UTR
CTTGTGTTGCAATGGCCGTC+TGG	0.310688	tig0033659:+25691	None:intergenic
TGCAATGGCCGTCTGGGTTA+GGG	0.312768	tig0033659:+25698	None:intergenic
AAGTTTCCTATTGTACAAAA+TGG	0.313559	tig0033659:+27398	None:intergenic
TCACTTTCACTACCAATTCA+TGG	0.317926	tig0033659:-27083	Msa1452060:CDS
AAGAAATTCTACCTGGATTC+AGG	0.317980	tig0033659:-26314	Msa1452060:CDS
TGTTAACATGATAATTTATT+GGG	0.320485	tig0033659:+27354	None:intergenic
GACCCTGTTGTAAAATTATC+AGG	0.320742	tig0033659:-26064	Msa1452060:CDS
ACAGAATGCTTGTTCCTAAA+AGG	0.329840	tig0033659:+26041	None:intergenic
GGGGTTTACTTCATGATAAA+TGG	0.338544	tig0033659:+25719	None:intergenic
TTATCTCCTGGGCATAGATT+AGG	0.368175	tig0033659:+25443	None:intergenic
ACAAAATGGTTCAATTGATT+TGG	0.377663	tig0033659:+27412	None:intergenic
AAAGGCTCGCTTTGACAGTT+CGG	0.382952	tig0033659:-25592	Msa1452060:CDS
TGTTTAGGATTATTCCTAAA+GGG	0.384551	tig0033659:-25174	Msa1452060:three_prime_UTR
TATCTCCTGGGCATAGATTA+GGG	0.396585	tig0033659:+25444	None:intergenic
TATCAACTGTAGCTATTGTT+TGG	0.400190	tig0033659:+27465	None:intergenic
CTGCATTCCAATCCATGAAT+TGG	0.405723	tig0033659:+27071	None:intergenic
TGTAACTATGGCCGGCGAAA+TGG	0.408333	tig0033659:-25523	Msa1452060:CDS
AAGGCTCGCTTTGACAGTTC+GGG	0.409065	tig0033659:-25591	Msa1452060:CDS
TTCACTACCAATTCATGGAT+TGG	0.415231	tig0033659:-27078	Msa1452060:CDS
AATGGTACCTTCCCGGAGTC+TGG	0.421783	tig0033659:+26270	None:intergenic
CATGTTAACAAATCACAAAA+AGG	0.423147	tig0033659:-27341	Msa1452060:CDS
ACCAAGTTGACTGCTGGCTT+CGG	0.424833	tig0033659:-25974	Msa1452060:CDS
GCAAGGCAACATTGGCTCCT+AGG	0.425523	tig0033659:+26017	None:intergenic
GAATGTTTGCTTGCATTTCA+GGG	0.428071	tig0033659:+25118	None:intergenic
TCTAATACTAGTACTGTTGT+TGG	0.428133	tig0033659:+25338	None:intergenic
CCTGGTGTATATTCTAATCT+TGG	0.430429	tig0033659:-27259	Msa1452060:CDS
GATAATTTATTGGGAGGGAA+AGG	0.435347	tig0033659:+27363	None:intergenic
CAATTCATGGATTGGAATGC+AGG	0.439436	tig0033659:-27070	Msa1452060:CDS
ACAAAGTAATGGTACCTTCC+CGG	0.441553	tig0033659:+26263	None:intergenic
ATATTCTAATCTTGGCGATA+AGG	0.441721	tig0033659:-27251	Msa1452060:CDS
AGCTTGTCACTCAAGGAAAC+TGG	0.442221	tig0033659:-25660	Msa1452060:CDS
ACTGGGATTACTGGATGCAT+TGG	0.448328	tig0033659:-26115	Msa1452060:CDS
AACTGAGAAGTTGCTGGATG+AGG	0.457094	tig0033659:-25408	Msa1452060:three_prime_UTR
GGAGGGAAAGGGAAGGCAAA+GGG	0.457212	tig0033659:+27375	None:intergenic
AAATGGTTCAATTGATTTGG+TGG	0.459417	tig0033659:+27415	None:intergenic
CAGGGTCATATGCTCCATCT+TGG	0.460843	tig0033659:+26080	None:intergenic
ACAAGGTCAATGAAGCTTCA+AGG	0.465289	tig0033659:+25936	None:intergenic
GCCATGGAGACTCCAACCTT+TGG	0.474824	tig0033659:+25474	None:intergenic
CAGTACTTGAATAGATTCAC+TGG	0.475573	tig0033659:-26133	Msa1452060:CDS
AACATGATAATTTATTGGGA+GGG	0.491687	tig0033659:+27358	None:intergenic
TAAAATGAAAGCTTATCTCC+TGG	0.495555	tig0033659:+25431	None:intergenic
AACTTGGTTATCGATCTTGT+TGG	0.497298	tig0033659:+25989	None:intergenic
TTTGACAGTTCGGGCAAAGT+AGG	0.498953	tig0033659:-25582	Msa1452060:CDS
AGCAAAATGAGCAAGAGTCC+TGG	0.500833	tig0033659:-27277	Msa1452060:CDS
GCAATGGCCGTCTGGGTTAG+GGG	0.501382	tig0033659:+25699	None:intergenic
CAAGGCAACATTGGCTCCTA+GGG	0.505987	tig0033659:+26018	None:intergenic
TTTATTAGATTGTCTTTACA+AGG	0.506198	tig0033659:-27128	Msa1452060:intron
ATAATTTATTGGGAGGGAAA+GGG	0.507024	tig0033659:+27364	None:intergenic
GCCGAAGCCAGCAGTCAACT+TGG	0.507792	tig0033659:+25973	None:intergenic
AATTGGTAGTGAAAGTGAAT+AGG	0.508379	tig0033659:+27088	None:intergenic
TTGTTTAGGATTATTCCTAA+AGG	0.515805	tig0033659:-25175	Msa1452060:three_prime_UTR
AAAATGAAAGCTTATCTCCT+GGG	0.518903	tig0033659:+25432	None:intergenic
TTGTGTTGCAATGGCCGTCT+GGG	0.533482	tig0033659:+25692	None:intergenic
GGAACAAGCATTCTGTCCCT+AGG	0.535981	tig0033659:-26034	Msa1452060:CDS
AAGGAAACTGGTGTCACCAT+TGG	0.538400	tig0033659:-25648	Msa1452060:CDS
AATGCAGGCTTCTCTTGTAA+AGG	0.539074	tig0033659:-27055	Msa1452060:intron
AAATGTACCGTACCAGACTC+CGG	0.544121	tig0033659:-26282	Msa1452060:CDS
CAATGGCCGTCTGGGTTAGG+GGG	0.545151	tig0033659:+25700	None:intergenic
TTGAAAACAGTCCTGAATCC+AGG	0.545165	tig0033659:+26303	None:intergenic
ACAGTTCAAGAAATTCTACC+TGG	0.548340	tig0033659:-26321	Msa1452060:intron
TCGATAACCAAGTTGACTGC+TGG	0.549363	tig0033659:-25980	Msa1452060:CDS
GGGAGGGAAAGGGAAGGCAA+AGG	0.554970	tig0033659:+27374	None:intergenic
GGCCCCAAAATCCATTTCGC+CGG	0.561250	tig0033659:+25512	None:intergenic
TAACATGATAATTTATTGGG+AGG	0.562148	tig0033659:+27357	None:intergenic
CGATCTTGTTGGTATGTGCA+AGG	0.562305	tig0033659:+26000	None:intergenic
AACCTTTGGAATCTTATCAT+CGG	0.566666	tig0033659:+25488	None:intergenic
AAATGCATGCTGAGCACCAA+TGG	0.571123	tig0033659:+25632	None:intergenic
GGATTATTAGGAAACCAAGA+TGG	0.572580	tig0033659:-26094	Msa1452060:CDS
GTTTAGGATTATTCCTAAAG+GGG	0.575216	tig0033659:-25173	Msa1452060:three_prime_UTR
TCCAAAGGTTGGAGTCTCCA+TGG	0.577690	tig0033659:-25475	Msa1452060:CDS
ATGCTTCACTTGTGTTGCAA+TGG	0.577920	tig0033659:+25683	None:intergenic
GATGATAAGATTCCAAAGGT+TGG	0.579129	tig0033659:-25486	Msa1452060:CDS
AATCTTGGCGATAAGGCCAA+AGG	0.579547	tig0033659:-27244	Msa1452060:intron
AAGCAAACATTCAGATTCTG+AGG	0.582598	tig0033659:-25107	Msa1452060:three_prime_UTR
AGATTAGGGTCTTAGAGCCA+TGG	0.589733	tig0033659:+25458	None:intergenic
TAAGACCCTAATCTATGCCC+AGG	0.590456	tig0033659:-25449	Msa1452060:exon
AATGTACCGTACCAGACTCC+GGG	0.592748	tig0033659:-26281	Msa1452060:CDS
CTTTGGAATCTTATCATCGG+TGG	0.598433	tig0033659:+25491	None:intergenic
GAAGCATAGCTTGTCACTCA+AGG	0.598774	tig0033659:-25667	Msa1452060:CDS
TACCTTCCCGGAGTCTGGTA+CGG	0.603084	tig0033659:+26275	None:intergenic
TTTATTGGGAGGGAAAGGGA+AGG	0.604666	tig0033659:+27368	None:intergenic
GGTCAATGAAGCTTCAAGGA+AGG	0.612698	tig0033659:+25940	None:intergenic
AGTACTTGAATAGATTCACT+GGG	0.614520	tig0033659:-26132	Msa1452060:CDS
TGGTATGTGCAAGGCAACAT+TGG	0.624437	tig0033659:+26009	None:intergenic
CCAAGATTAGAATATACACC+AGG	0.632111	tig0033659:+27259	None:intergenic
AGCAAACATTCAGATTCTGA+GGG	0.649808	tig0033659:-25106	Msa1452060:three_prime_UTR
CACCGATGATAAGATTCCAA+AGG	0.660499	tig0033659:-25490	Msa1452060:CDS
TACCGTACCAGACTCCGGGA+AGG	0.677553	tig0033659:-26277	Msa1452060:intron
GTAGGTGCTCTCATTCAACA+AGG	0.711960	tig0033659:-25564	Msa1452060:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTTAACATGATAATTTATT+GGG	+	tig0033659:25212-25231	None:intergenic	15.0%
!!	TTGTTAACATGATAATTTAT+TGG	+	tig0033659:25213-25232	None:intergenic	15.0%
!!!	TACTTTTATTTTTCTACTTA+TGG	-	tig0033659:25642-25661	Msa1452060:CDS	15.0%
!!	ATATCACATTATTTAAATCA+AGG	-	tig0033659:25876-25895	Msa1452060:intron	15.0%
!!	TATCACATTATTTAAATCAA+GGG	-	tig0033659:25877-25896	Msa1452060:intron	15.0%
!!!	TTTCTATACATATTTCTAAA+CGG	+	tig0033659:26101-26120	None:intergenic	15.0%
!!!	ATTTTTCTGATAAATTTAGT+CGG	+	tig0033659:26675-26694	None:intergenic	15.0%
!!	TTAAATATTTCATATTATGC+AGG	-	tig0033659:26793-26812	Msa1452060:intron	15.0%
!!	TGTTAACATGATAATTTATT+GGG	+	tig0033659:25212-25231	None:intergenic	15.0%
!!	TTGTTAACATGATAATTTAT+TGG	+	tig0033659:25213-25232	None:intergenic	15.0%
!!!	TACTTTTATTTTTCTACTTA+TGG	-	tig0033659:25642-25661	Msa1452060:CDS	15.0%
!!	ATATCACATTATTTAAATCA+AGG	-	tig0033659:25876-25895	Msa1452060:intron	15.0%
!!	TATCACATTATTTAAATCAA+GGG	-	tig0033659:25877-25896	Msa1452060:intron	15.0%
!!!	TTTCTATACATATTTCTAAA+CGG	+	tig0033659:26101-26120	None:intergenic	15.0%
!!!	ATTTTTCTGATAAATTTAGT+CGG	+	tig0033659:26675-26694	None:intergenic	15.0%
!!	TTAAATATTTCATATTATGC+AGG	-	tig0033659:26793-26812	Msa1452060:intron	15.0%
!!	ATTTCAGTATAATACTAGTA+AGG	+	tig0033659:25401-25420	None:intergenic	20.0%
!!	TTTATTAGATTGTCTTTACA+AGG	-	tig0033659:25435-25454	Msa1452060:three_prime_UTR	20.0%
!!!	TCATTGAATGTATTTTGTTA+TGG	+	tig0033659:25936-25955	None:intergenic	20.0%
!!	TGATACTATACATAGTATAT+AGG	+	tig0033659:26331-26350	None:intergenic	20.0%
!!	GACTAAATTTATCAGAAAAA+TGG	-	tig0033659:26674-26693	Msa1452060:intron	20.0%
!!	AATTTATCAGAAAAATGGAT+AGG	-	tig0033659:26679-26698	Msa1452060:intron	20.0%
!!	ATTTCAGTATAATACTAGTA+AGG	+	tig0033659:25401-25420	None:intergenic	20.0%
!!	TTTATTAGATTGTCTTTACA+AGG	-	tig0033659:25435-25454	Msa1452060:three_prime_UTR	20.0%
!!!	TCATTGAATGTATTTTGTTA+TGG	+	tig0033659:25936-25955	None:intergenic	20.0%
!!	TGATACTATACATAGTATAT+AGG	+	tig0033659:26331-26350	None:intergenic	20.0%
!!	GACTAAATTTATCAGAAAAA+TGG	-	tig0033659:26674-26693	Msa1452060:intron	20.0%
!!	AATTTATCAGAAAAATGGAT+AGG	-	tig0033659:26679-26698	Msa1452060:intron	20.0%
!!	TGGAAATTTCATTTTATGCA+TGG	-	tig0033659:25123-25142	Msa1452060:three_prime_UTR	25.0%
!	ACAAAATGGTTCAATTGATT+TGG	+	tig0033659:25154-25173	None:intergenic	25.0%
!!	ATTGAACCATTTTGTACAAT+AGG	-	tig0033659:25159-25178	Msa1452060:three_prime_UTR	25.0%
!	AAGTTTCCTATTGTACAAAA+TGG	+	tig0033659:25168-25187	None:intergenic	25.0%
!	AACATGATAATTTATTGGGA+GGG	+	tig0033659:25208-25227	None:intergenic	25.0%
!	TAACATGATAATTTATTGGG+AGG	+	tig0033659:25209-25228	None:intergenic	25.0%
!	CATGTTAACAAATCACAAAA+AGG	-	tig0033659:25222-25241	Msa1452060:three_prime_UTR	25.0%
!	ATGGAATTTGATAAAACCTT+TGG	+	tig0033659:25338-25357	None:intergenic	25.0%
!!	TTCTAAAGATTCAAATGGAA+TGG	+	tig0033659:25357-25376	None:intergenic	25.0%
!	ATATCAACTTAGCTTATGTT+TGG	-	tig0033659:25539-25558	Msa1452060:CDS	25.0%
!	GAGAAAGTTAGAAAAATTTG+AGG	+	tig0033659:25794-25813	None:intergenic	25.0%
!	AAGATAGAAAATGGAATGAT+GGG	+	tig0033659:25816-25835	None:intergenic	25.0%
!	AAAGATAGAAAATGGAATGA+TGG	+	tig0033659:25817-25836	None:intergenic	25.0%
!	AGACTACAAAAGATAGAAAA+TGG	+	tig0033659:25825-25844	None:intergenic	25.0%
!	CAATCTTCATTTCATATGAT+TGG	-	tig0033659:26010-26029	Msa1452060:CDS	25.0%
!!	AGGGTTCTAATAAATTAACA+TGG	+	tig0033659:26162-26181	None:intergenic	25.0%
!	TAGAATTTCTTGAACTGTAA+TGG	+	tig0033659:26240-26259	None:intergenic	25.0%
!	TATAGGTAAGTACAAAGTAA+TGG	+	tig0033659:26314-26333	None:intergenic	25.0%
!	TATGAATTCATTGTGATTTG+AGG	+	tig0033659:26400-26419	None:intergenic	25.0%
!	TCAAATCACAATGAATTCAT+AGG	-	tig0033659:26399-26418	Msa1452060:intron	25.0%
!!!	TATGATGGATATATCTTACA+AGG	+	tig0033659:26647-26666	None:intergenic	25.0%
!	TTTCAAACAATGACACAAAT+TGG	-	tig0033659:27182-27201	Msa1452060:intron	25.0%
!!	TACTTTTCTTAGAGTTTTAC+TGG	-	tig0033659:27338-27357	Msa1452060:CDS	25.0%
!!	TTGTTTAGGATTATTCCTAA+AGG	-	tig0033659:27388-27407	Msa1452060:five_prime_UTR	25.0%
!!	TGTTTAGGATTATTCCTAAA+GGG	-	tig0033659:27389-27408	Msa1452060:five_prime_UTR	25.0%
!!	TGGAAATTTCATTTTATGCA+TGG	-	tig0033659:25123-25142	Msa1452060:three_prime_UTR	25.0%
!	ACAAAATGGTTCAATTGATT+TGG	+	tig0033659:25154-25173	None:intergenic	25.0%
!!	ATTGAACCATTTTGTACAAT+AGG	-	tig0033659:25159-25178	Msa1452060:three_prime_UTR	25.0%
!	AAGTTTCCTATTGTACAAAA+TGG	+	tig0033659:25168-25187	None:intergenic	25.0%
!	AACATGATAATTTATTGGGA+GGG	+	tig0033659:25208-25227	None:intergenic	25.0%
!	TAACATGATAATTTATTGGG+AGG	+	tig0033659:25209-25228	None:intergenic	25.0%
!	CATGTTAACAAATCACAAAA+AGG	-	tig0033659:25222-25241	Msa1452060:three_prime_UTR	25.0%
!	ATGGAATTTGATAAAACCTT+TGG	+	tig0033659:25338-25357	None:intergenic	25.0%
!!	TTCTAAAGATTCAAATGGAA+TGG	+	tig0033659:25357-25376	None:intergenic	25.0%
!	ATATCAACTTAGCTTATGTT+TGG	-	tig0033659:25539-25558	Msa1452060:CDS	25.0%
!	GAGAAAGTTAGAAAAATTTG+AGG	+	tig0033659:25794-25813	None:intergenic	25.0%
!	AAGATAGAAAATGGAATGAT+GGG	+	tig0033659:25816-25835	None:intergenic	25.0%
!	AAAGATAGAAAATGGAATGA+TGG	+	tig0033659:25817-25836	None:intergenic	25.0%
!	AGACTACAAAAGATAGAAAA+TGG	+	tig0033659:25825-25844	None:intergenic	25.0%
!	CAATCTTCATTTCATATGAT+TGG	-	tig0033659:26010-26029	Msa1452060:CDS	25.0%
!!	AGGGTTCTAATAAATTAACA+TGG	+	tig0033659:26162-26181	None:intergenic	25.0%
!	TAGAATTTCTTGAACTGTAA+TGG	+	tig0033659:26240-26259	None:intergenic	25.0%
!	TATAGGTAAGTACAAAGTAA+TGG	+	tig0033659:26314-26333	None:intergenic	25.0%
!	TATGAATTCATTGTGATTTG+AGG	+	tig0033659:26400-26419	None:intergenic	25.0%
!	TCAAATCACAATGAATTCAT+AGG	-	tig0033659:26399-26418	Msa1452060:intron	25.0%
!!!	TATGATGGATATATCTTACA+AGG	+	tig0033659:26647-26666	None:intergenic	25.0%
!	TTTCAAACAATGACACAAAT+TGG	-	tig0033659:27182-27201	Msa1452060:intron	25.0%
!!	TACTTTTCTTAGAGTTTTAC+TGG	-	tig0033659:27338-27357	Msa1452060:CDS	25.0%
!!	TTGTTTAGGATTATTCCTAA+AGG	-	tig0033659:27388-27407	Msa1452060:five_prime_UTR	25.0%
!!	TGTTTAGGATTATTCCTAAA+GGG	-	tig0033659:27389-27408	Msa1452060:five_prime_UTR	25.0%
	TATCAACTGTAGCTATTGTT+TGG	+	tig0033659:25101-25120	None:intergenic	30.0%
!	CAATAGCTACAGTTGATATT+TGG	-	tig0033659:25103-25122	Msa1452060:three_prime_UTR	30.0%
!!	AAATGGTTCAATTGATTTGG+TGG	+	tig0033659:25151-25170	None:intergenic	30.0%
	ATAATTTATTGGGAGGGAAA+GGG	+	tig0033659:25202-25221	None:intergenic	30.0%
	ATATTCTAATCTTGGCGATA+AGG	-	tig0033659:25312-25331	Msa1452060:three_prime_UTR	30.0%
!	CACTCTTCTAAAGATTCAAA+TGG	+	tig0033659:25362-25381	None:intergenic	30.0%
!!	AATTGGTAGTGAAAGTGAAT+AGG	+	tig0033659:25478-25497	None:intergenic	30.0%
	TAAAAGATTAAGCCTCTGTT+TGG	+	tig0033659:25624-25643	None:intergenic	30.0%
	TTAGCCAAATGTTATTCTGT+TGG	-	tig0033659:25689-25708	Msa1452060:CDS	30.0%
	ACTTCCAACAGAATAACATT+TGG	+	tig0033659:25696-25715	None:intergenic	30.0%
	ATGTTATTCTGTTGGAAGTA+TGG	-	tig0033659:25697-25716	Msa1452060:CDS	30.0%
	CACATTATTTAAATCAAGGG+TGG	-	tig0033659:25880-25899	Msa1452060:intron	30.0%
	TATTAGAACCCTGAATGAAA+TGG	-	tig0033659:26170-26189	Msa1452060:intron	30.0%
	AATCACAATGAATTCATAGG+TGG	-	tig0033659:26402-26421	Msa1452060:intron	30.0%
	AGTACTTGAATAGATTCACT+GGG	-	tig0033659:26431-26450	Msa1452060:intron	30.0%
!	GTAAAATTATCAGGCCTTTT+AGG	-	tig0033659:26508-26527	Msa1452060:intron	30.0%
	ATTTAGTCGGTAGTTTATGA+TGG	+	tig0033659:26662-26681	None:intergenic	30.0%
!	TTTTCCTGAAACATTGCTAA+AGG	-	tig0033659:26953-26972	Msa1452060:intron	30.0%
!!!	GCAGAGATTTTTTGTAACTA+TGG	-	tig0033659:27028-27047	Msa1452060:intron	30.0%
!!!	AGATTTTTTGTAACTATGGC+CGG	-	tig0033659:27032-27051	Msa1452060:intron	30.0%
!!	AACCTTTGGAATCTTATCAT+CGG	+	tig0033659:27078-27097	None:intergenic	30.0%
	AAAATGAAAGCTTATCTCCT+GGG	+	tig0033659:27134-27153	None:intergenic	30.0%
	TAAAATGAAAGCTTATCTCC+TGG	+	tig0033659:27135-27154	None:intergenic	30.0%
!	ATTTTAAACTGAGAAGTTGC+TGG	-	tig0033659:27149-27168	Msa1452060:intron	30.0%
	TCTAATACTAGTACTGTTGT+TGG	+	tig0033659:27228-27247	None:intergenic	30.0%
!	GTTTAGGATTATTCCTAAAG+GGG	-	tig0033659:27390-27409	Msa1452060:five_prime_UTR	30.0%
	TATCAACTGTAGCTATTGTT+TGG	+	tig0033659:25101-25120	None:intergenic	30.0%
!	CAATAGCTACAGTTGATATT+TGG	-	tig0033659:25103-25122	Msa1452060:three_prime_UTR	30.0%
!!	AAATGGTTCAATTGATTTGG+TGG	+	tig0033659:25151-25170	None:intergenic	30.0%
	ATAATTTATTGGGAGGGAAA+GGG	+	tig0033659:25202-25221	None:intergenic	30.0%
	ATATTCTAATCTTGGCGATA+AGG	-	tig0033659:25312-25331	Msa1452060:three_prime_UTR	30.0%
!	CACTCTTCTAAAGATTCAAA+TGG	+	tig0033659:25362-25381	None:intergenic	30.0%
!!	AATTGGTAGTGAAAGTGAAT+AGG	+	tig0033659:25478-25497	None:intergenic	30.0%
	TAAAAGATTAAGCCTCTGTT+TGG	+	tig0033659:25624-25643	None:intergenic	30.0%
	TTAGCCAAATGTTATTCTGT+TGG	-	tig0033659:25689-25708	Msa1452060:CDS	30.0%
	ACTTCCAACAGAATAACATT+TGG	+	tig0033659:25696-25715	None:intergenic	30.0%
	ATGTTATTCTGTTGGAAGTA+TGG	-	tig0033659:25697-25716	Msa1452060:CDS	30.0%
	CACATTATTTAAATCAAGGG+TGG	-	tig0033659:25880-25899	Msa1452060:intron	30.0%
	TATTAGAACCCTGAATGAAA+TGG	-	tig0033659:26170-26189	Msa1452060:intron	30.0%
	AATCACAATGAATTCATAGG+TGG	-	tig0033659:26402-26421	Msa1452060:intron	30.0%
	AGTACTTGAATAGATTCACT+GGG	-	tig0033659:26431-26450	Msa1452060:intron	30.0%
!	GTAAAATTATCAGGCCTTTT+AGG	-	tig0033659:26508-26527	Msa1452060:intron	30.0%
	ATTTAGTCGGTAGTTTATGA+TGG	+	tig0033659:26662-26681	None:intergenic	30.0%
!	TTTTCCTGAAACATTGCTAA+AGG	-	tig0033659:26953-26972	Msa1452060:intron	30.0%
!!!	GCAGAGATTTTTTGTAACTA+TGG	-	tig0033659:27028-27047	Msa1452060:intron	30.0%
!!!	AGATTTTTTGTAACTATGGC+CGG	-	tig0033659:27032-27051	Msa1452060:intron	30.0%
!!	AACCTTTGGAATCTTATCAT+CGG	+	tig0033659:27078-27097	None:intergenic	30.0%
	AAAATGAAAGCTTATCTCCT+GGG	+	tig0033659:27134-27153	None:intergenic	30.0%
	TAAAATGAAAGCTTATCTCC+TGG	+	tig0033659:27135-27154	None:intergenic	30.0%
!	ATTTTAAACTGAGAAGTTGC+TGG	-	tig0033659:27149-27168	Msa1452060:intron	30.0%
	TCTAATACTAGTACTGTTGT+TGG	+	tig0033659:27228-27247	None:intergenic	30.0%
!	GTTTAGGATTATTCCTAAAG+GGG	-	tig0033659:27390-27409	Msa1452060:five_prime_UTR	30.0%
	GATAATTTATTGGGAGGGAA+AGG	+	tig0033659:25203-25222	None:intergenic	35.0%
	CCAAGATTAGAATATACACC+AGG	+	tig0033659:25307-25326	None:intergenic	35.0%
	CCTGGTGTATATTCTAATCT+TGG	-	tig0033659:25304-25323	Msa1452060:three_prime_UTR	35.0%
	TCACTTTCACTACCAATTCA+TGG	-	tig0033659:25480-25499	Msa1452060:CDS	35.0%
	TTCACTACCAATTCATGGAT+TGG	-	tig0033659:25485-25504	Msa1452060:CDS	35.0%
!	TTAGCTTATGTTTGGAACTG+CGG	-	tig0033659:25547-25566	Msa1452060:CDS	35.0%
	TTTAGACTGCAAGAATGAGA+AGG	-	tig0033659:25587-25606	Msa1452060:CDS	35.0%
	TGGCATCTAAATTTGCACTT+AGG	-	tig0033659:26030-26049	Msa1452060:CDS	35.0%
!	AGGTAATGCCATTTCATTCA+GGG	+	tig0033659:26181-26200	None:intergenic	35.0%
	ACAGTTCAAGAAATTCTACC+TGG	-	tig0033659:26242-26261	Msa1452060:intron	35.0%
	AAGAAATTCTACCTGGATTC+AGG	-	tig0033659:26249-26268	Msa1452060:intron	35.0%
	CAGTACTTGAATAGATTCAC+TGG	-	tig0033659:26430-26449	Msa1452060:intron	35.0%
	AATAGATTCACTGGGATTAC+TGG	-	tig0033659:26439-26458	Msa1452060:intron	35.0%
	ACTGGATGCATTGGATTATT+AGG	-	tig0033659:26457-26476	Msa1452060:intron	35.0%
	GGATTATTAGGAAACCAAGA+TGG	-	tig0033659:26469-26488	Msa1452060:intron	35.0%
	GACCCTGTTGTAAAATTATC+AGG	-	tig0033659:26499-26518	Msa1452060:intron	35.0%
!	GGCCTGATAATTTTACAACA+GGG	+	tig0033659:26504-26523	None:intergenic	35.0%
!	AGGCCTGATAATTTTACAAC+AGG	+	tig0033659:26505-26524	None:intergenic	35.0%
	ACAGAATGCTTGTTCCTAAA+AGG	+	tig0033659:26525-26544	None:intergenic	35.0%
	AACTTGGTTATCGATCTTGT+TGG	+	tig0033659:26577-26596	None:intergenic	35.0%
	GGGGTTTACTTCATGATAAA+TGG	+	tig0033659:26847-26866	None:intergenic	35.0%
!	CTCTCATTCAACAAGGATTT+TGG	-	tig0033659:27006-27025	Msa1452060:intron	35.0%
	GATGATAAGATTCCAAAGGT+TGG	-	tig0033659:27077-27096	Msa1452060:CDS	35.0%
	CTTCACTTTCGACATTGTTT+AGG	-	tig0033659:27374-27393	Msa1452060:five_prime_UTR	35.0%
	ATAAATCTTCTCTCCCCTTT+AGG	+	tig0033659:27406-27425	None:intergenic	35.0%
	GAATGTTTGCTTGCATTTCA+GGG	+	tig0033659:27448-27467	None:intergenic	35.0%
	TGAATGTTTGCTTGCATTTC+AGG	+	tig0033659:27449-27468	None:intergenic	35.0%
	AAGCAAACATTCAGATTCTG+AGG	-	tig0033659:27456-27475	Msa1452060:five_prime_UTR	35.0%
	AGCAAACATTCAGATTCTGA+GGG	-	tig0033659:27457-27476	Msa1452060:five_prime_UTR	35.0%
	GATAATTTATTGGGAGGGAA+AGG	+	tig0033659:25203-25222	None:intergenic	35.0%
	CCAAGATTAGAATATACACC+AGG	+	tig0033659:25307-25326	None:intergenic	35.0%
	CCTGGTGTATATTCTAATCT+TGG	-	tig0033659:25304-25323	Msa1452060:three_prime_UTR	35.0%
	TCACTTTCACTACCAATTCA+TGG	-	tig0033659:25480-25499	Msa1452060:CDS	35.0%
	TTCACTACCAATTCATGGAT+TGG	-	tig0033659:25485-25504	Msa1452060:CDS	35.0%
!	TTAGCTTATGTTTGGAACTG+CGG	-	tig0033659:25547-25566	Msa1452060:CDS	35.0%
	TTTAGACTGCAAGAATGAGA+AGG	-	tig0033659:25587-25606	Msa1452060:CDS	35.0%
	TGGCATCTAAATTTGCACTT+AGG	-	tig0033659:26030-26049	Msa1452060:CDS	35.0%
!	AGGTAATGCCATTTCATTCA+GGG	+	tig0033659:26181-26200	None:intergenic	35.0%
	ACAGTTCAAGAAATTCTACC+TGG	-	tig0033659:26242-26261	Msa1452060:intron	35.0%
	AAGAAATTCTACCTGGATTC+AGG	-	tig0033659:26249-26268	Msa1452060:intron	35.0%
	CAGTACTTGAATAGATTCAC+TGG	-	tig0033659:26430-26449	Msa1452060:intron	35.0%
	AATAGATTCACTGGGATTAC+TGG	-	tig0033659:26439-26458	Msa1452060:intron	35.0%
	ACTGGATGCATTGGATTATT+AGG	-	tig0033659:26457-26476	Msa1452060:intron	35.0%
	GGATTATTAGGAAACCAAGA+TGG	-	tig0033659:26469-26488	Msa1452060:intron	35.0%
	GACCCTGTTGTAAAATTATC+AGG	-	tig0033659:26499-26518	Msa1452060:intron	35.0%
!	GGCCTGATAATTTTACAACA+GGG	+	tig0033659:26504-26523	None:intergenic	35.0%
!	AGGCCTGATAATTTTACAAC+AGG	+	tig0033659:26505-26524	None:intergenic	35.0%
	ACAGAATGCTTGTTCCTAAA+AGG	+	tig0033659:26525-26544	None:intergenic	35.0%
	AACTTGGTTATCGATCTTGT+TGG	+	tig0033659:26577-26596	None:intergenic	35.0%
	GGGGTTTACTTCATGATAAA+TGG	+	tig0033659:26847-26866	None:intergenic	35.0%
!	CTCTCATTCAACAAGGATTT+TGG	-	tig0033659:27006-27025	Msa1452060:intron	35.0%
	GATGATAAGATTCCAAAGGT+TGG	-	tig0033659:27077-27096	Msa1452060:CDS	35.0%
	CTTCACTTTCGACATTGTTT+AGG	-	tig0033659:27374-27393	Msa1452060:five_prime_UTR	35.0%
	ATAAATCTTCTCTCCCCTTT+AGG	+	tig0033659:27406-27425	None:intergenic	35.0%
	GAATGTTTGCTTGCATTTCA+GGG	+	tig0033659:27448-27467	None:intergenic	35.0%
	TGAATGTTTGCTTGCATTTC+AGG	+	tig0033659:27449-27468	None:intergenic	35.0%
	AAGCAAACATTCAGATTCTG+AGG	-	tig0033659:27456-27475	Msa1452060:five_prime_UTR	35.0%
	AGCAAACATTCAGATTCTGA+GGG	-	tig0033659:27457-27476	Msa1452060:five_prime_UTR	35.0%
	CTGCATTCCAATCCATGAAT+TGG	+	tig0033659:25495-25514	None:intergenic	40.0%
!!	CAATTCATGGATTGGAATGC+AGG	-	tig0033659:25493-25512	Msa1452060:CDS	40.0%
	AATGCAGGCTTCTCTTGTAA+AGG	-	tig0033659:25508-25527	Msa1452060:CDS	40.0%
	AGAAACAGTTTAGCCCTCAA+AGG	-	tig0033659:26116-26135	Msa1452060:CDS	40.0%
	TTGTTTGTGGAGACCTTTGA+GGG	+	tig0033659:26132-26151	None:intergenic	40.0%
	TTTGTTTGTGGAGACCTTTG+AGG	+	tig0033659:26133-26152	None:intergenic	40.0%
	ACATGGCTCTCATTTGTTTG+TGG	+	tig0033659:26145-26164	None:intergenic	40.0%
!	CAGGTAATGCCATTTCATTC+AGG	+	tig0033659:26182-26201	None:intergenic	40.0%
	TTGAAAACAGTCCTGAATCC+AGG	+	tig0033659:26263-26282	None:intergenic	40.0%
	ACAAAGTAATGGTACCTTCC+CGG	+	tig0033659:26303-26322	None:intergenic	40.0%
	ACAAGGTCAATGAAGCTTCA+AGG	+	tig0033659:26630-26649	None:intergenic	40.0%
	ATGCTTCACTTGTGTTGCAA+TGG	+	tig0033659:26883-26902	None:intergenic	40.0%
!!	CTTTGGAATCTTATCATCGG+TGG	+	tig0033659:27075-27094	None:intergenic	40.0%
	CACCGATGATAAGATTCCAA+AGG	-	tig0033659:27073-27092	Msa1452060:CDS	40.0%
	TATCTCCTGGGCATAGATTA+GGG	+	tig0033659:27122-27141	None:intergenic	40.0%
	TTATCTCCTGGGCATAGATT+AGG	+	tig0033659:27123-27142	None:intergenic	40.0%
	CTGCATTCCAATCCATGAAT+TGG	+	tig0033659:25495-25514	None:intergenic	40.0%
!!	CAATTCATGGATTGGAATGC+AGG	-	tig0033659:25493-25512	Msa1452060:CDS	40.0%
	AATGCAGGCTTCTCTTGTAA+AGG	-	tig0033659:25508-25527	Msa1452060:CDS	40.0%
	AGAAACAGTTTAGCCCTCAA+AGG	-	tig0033659:26116-26135	Msa1452060:CDS	40.0%
	TTGTTTGTGGAGACCTTTGA+GGG	+	tig0033659:26132-26151	None:intergenic	40.0%
	TTTGTTTGTGGAGACCTTTG+AGG	+	tig0033659:26133-26152	None:intergenic	40.0%
	ACATGGCTCTCATTTGTTTG+TGG	+	tig0033659:26145-26164	None:intergenic	40.0%
!	CAGGTAATGCCATTTCATTC+AGG	+	tig0033659:26182-26201	None:intergenic	40.0%
	TTGAAAACAGTCCTGAATCC+AGG	+	tig0033659:26263-26282	None:intergenic	40.0%
	ACAAAGTAATGGTACCTTCC+CGG	+	tig0033659:26303-26322	None:intergenic	40.0%
	ACAAGGTCAATGAAGCTTCA+AGG	+	tig0033659:26630-26649	None:intergenic	40.0%
	ATGCTTCACTTGTGTTGCAA+TGG	+	tig0033659:26883-26902	None:intergenic	40.0%
!!	CTTTGGAATCTTATCATCGG+TGG	+	tig0033659:27075-27094	None:intergenic	40.0%
	CACCGATGATAAGATTCCAA+AGG	-	tig0033659:27073-27092	Msa1452060:CDS	40.0%
	TATCTCCTGGGCATAGATTA+GGG	+	tig0033659:27122-27141	None:intergenic	40.0%
	TTATCTCCTGGGCATAGATT+AGG	+	tig0033659:27123-27142	None:intergenic	40.0%
	TTTATTGGGAGGGAAAGGGA+AGG	+	tig0033659:25198-25217	None:intergenic	45.0%
	AGCAAAATGAGCAAGAGTCC+TGG	-	tig0033659:25286-25305	Msa1452060:three_prime_UTR	45.0%
	AATCTTGGCGATAAGGCCAA+AGG	-	tig0033659:25319-25338	Msa1452060:three_prime_UTR	45.0%
!	ATGTTTGGAACTGCGGTGAT+CGG	-	tig0033659:25554-25573	Msa1452060:CDS	45.0%
	AAATGTACCGTACCAGACTC+CGG	-	tig0033659:26281-26300	Msa1452060:CDS	45.0%
	ACTGGGATTACTGGATGCAT+TGG	-	tig0033659:26448-26467	Msa1452060:intron	45.0%
	TGGTATGTGCAAGGCAACAT+TGG	+	tig0033659:26557-26576	None:intergenic	45.0%
	CGATCTTGTTGGTATGTGCA+AGG	+	tig0033659:26566-26585	None:intergenic	45.0%
!	TCGATAACCAAGTTGACTGC+TGG	-	tig0033659:26583-26602	Msa1452060:intron	45.0%
	GGTCAATGAAGCTTCAAGGA+AGG	+	tig0033659:26626-26645	None:intergenic	45.0%
!	GAAGCATAGCTTGTCACTCA+AGG	-	tig0033659:26896-26915	Msa1452060:intron	45.0%
	AGCTTGTCACTCAAGGAAAC+TGG	-	tig0033659:26903-26922	Msa1452060:intron	45.0%
!!	AAGGAAACTGGTGTCACCAT+TGG	-	tig0033659:26915-26934	Msa1452060:intron	45.0%
	AAATGCATGCTGAGCACCAA+TGG	+	tig0033659:26934-26953	None:intergenic	45.0%
	CGAGCCTTTAGCAATGTTTC+AGG	+	tig0033659:26960-26979	None:intergenic	45.0%
	AAAGGCTCGCTTTGACAGTT+CGG	-	tig0033659:26971-26990	Msa1452060:intron	45.0%
	TTTGACAGTTCGGGCAAAGT+AGG	-	tig0033659:26981-27000	Msa1452060:intron	45.0%
	GTAGGTGCTCTCATTCAACA+AGG	-	tig0033659:26999-27018	Msa1452060:intron	45.0%
	AGATTAGGGTCTTAGAGCCA+TGG	+	tig0033659:27108-27127	None:intergenic	45.0%
	TAAGACCCTAATCTATGCCC+AGG	-	tig0033659:27114-27133	Msa1452060:CDS	45.0%
	AACTGAGAAGTTGCTGGATG+AGG	-	tig0033659:27155-27174	Msa1452060:intron	45.0%
	TTTATTGGGAGGGAAAGGGA+AGG	+	tig0033659:25198-25217	None:intergenic	45.0%
	AGCAAAATGAGCAAGAGTCC+TGG	-	tig0033659:25286-25305	Msa1452060:three_prime_UTR	45.0%
	AATCTTGGCGATAAGGCCAA+AGG	-	tig0033659:25319-25338	Msa1452060:three_prime_UTR	45.0%
!	ATGTTTGGAACTGCGGTGAT+CGG	-	tig0033659:25554-25573	Msa1452060:CDS	45.0%
	AAATGTACCGTACCAGACTC+CGG	-	tig0033659:26281-26300	Msa1452060:CDS	45.0%
	ACTGGGATTACTGGATGCAT+TGG	-	tig0033659:26448-26467	Msa1452060:intron	45.0%
	TGGTATGTGCAAGGCAACAT+TGG	+	tig0033659:26557-26576	None:intergenic	45.0%
	CGATCTTGTTGGTATGTGCA+AGG	+	tig0033659:26566-26585	None:intergenic	45.0%
!	TCGATAACCAAGTTGACTGC+TGG	-	tig0033659:26583-26602	Msa1452060:intron	45.0%
	GGTCAATGAAGCTTCAAGGA+AGG	+	tig0033659:26626-26645	None:intergenic	45.0%
!	GAAGCATAGCTTGTCACTCA+AGG	-	tig0033659:26896-26915	Msa1452060:intron	45.0%
	AGCTTGTCACTCAAGGAAAC+TGG	-	tig0033659:26903-26922	Msa1452060:intron	45.0%
!!	AAGGAAACTGGTGTCACCAT+TGG	-	tig0033659:26915-26934	Msa1452060:intron	45.0%
	AAATGCATGCTGAGCACCAA+TGG	+	tig0033659:26934-26953	None:intergenic	45.0%
	CGAGCCTTTAGCAATGTTTC+AGG	+	tig0033659:26960-26979	None:intergenic	45.0%
	AAAGGCTCGCTTTGACAGTT+CGG	-	tig0033659:26971-26990	Msa1452060:intron	45.0%
	TTTGACAGTTCGGGCAAAGT+AGG	-	tig0033659:26981-27000	Msa1452060:intron	45.0%
	GTAGGTGCTCTCATTCAACA+AGG	-	tig0033659:26999-27018	Msa1452060:intron	45.0%
	AGATTAGGGTCTTAGAGCCA+TGG	+	tig0033659:27108-27127	None:intergenic	45.0%
	TAAGACCCTAATCTATGCCC+AGG	-	tig0033659:27114-27133	Msa1452060:CDS	45.0%
	AACTGAGAAGTTGCTGGATG+AGG	-	tig0033659:27155-27174	Msa1452060:intron	45.0%
	CAAGAATGAGAAGGCTACCG+CGG	-	tig0033659:25596-25615	Msa1452060:CDS	50.0%
	TAAGCCTCTGTTTGGATCCG+CGG	+	tig0033659:25616-25635	None:intergenic	50.0%
	ACTACAGTCAGACATGCTGC+AGG	+	tig0033659:26201-26220	None:intergenic	50.0%
	AATGTACCGTACCAGACTCC+GGG	-	tig0033659:26282-26301	Msa1452060:CDS	50.0%
	CAGGGTCATATGCTCCATCT+TGG	+	tig0033659:26486-26505	None:intergenic	50.0%
	GGAACAAGCATTCTGTCCCT+AGG	-	tig0033659:26529-26548	Msa1452060:intron	50.0%
!!	CAAGGCAACATTGGCTCCTA+GGG	+	tig0033659:26548-26567	None:intergenic	50.0%
!!	ACCAAGTTGACTGCTGGCTT+CGG	-	tig0033659:26589-26608	Msa1452060:intron	50.0%
	AGTAAACCCCCTAACCCAGA+CGG	-	tig0033659:26857-26876	Msa1452060:intron	50.0%
	TTGTGTTGCAATGGCCGTCT+GGG	+	tig0033659:26874-26893	None:intergenic	50.0%
	AAGGCTCGCTTTGACAGTTC+GGG	-	tig0033659:26972-26991	Msa1452060:intron	50.0%
	TGTAACTATGGCCGGCGAAA+TGG	-	tig0033659:27040-27059	Msa1452060:intron	50.0%
!!	ATGGCCGGCGAAATGGATTT+TGG	-	tig0033659:27047-27066	Msa1452060:intron	50.0%
!!	TGGCCGGCGAAATGGATTTT+GGG	-	tig0033659:27048-27067	Msa1452060:intron	50.0%
	TCCAAAGGTTGGAGTCTCCA+TGG	-	tig0033659:27088-27107	Msa1452060:CDS	50.0%
	CAAGAATGAGAAGGCTACCG+CGG	-	tig0033659:25596-25615	Msa1452060:CDS	50.0%
	TAAGCCTCTGTTTGGATCCG+CGG	+	tig0033659:25616-25635	None:intergenic	50.0%
	ACTACAGTCAGACATGCTGC+AGG	+	tig0033659:26201-26220	None:intergenic	50.0%
	AATGTACCGTACCAGACTCC+GGG	-	tig0033659:26282-26301	Msa1452060:CDS	50.0%
	CAGGGTCATATGCTCCATCT+TGG	+	tig0033659:26486-26505	None:intergenic	50.0%
	GGAACAAGCATTCTGTCCCT+AGG	-	tig0033659:26529-26548	Msa1452060:intron	50.0%
!!	CAAGGCAACATTGGCTCCTA+GGG	+	tig0033659:26548-26567	None:intergenic	50.0%
!!	ACCAAGTTGACTGCTGGCTT+CGG	-	tig0033659:26589-26608	Msa1452060:intron	50.0%
	AGTAAACCCCCTAACCCAGA+CGG	-	tig0033659:26857-26876	Msa1452060:intron	50.0%
	TTGTGTTGCAATGGCCGTCT+GGG	+	tig0033659:26874-26893	None:intergenic	50.0%
	AAGGCTCGCTTTGACAGTTC+GGG	-	tig0033659:26972-26991	Msa1452060:intron	50.0%
	TGTAACTATGGCCGGCGAAA+TGG	-	tig0033659:27040-27059	Msa1452060:intron	50.0%
!!	ATGGCCGGCGAAATGGATTT+TGG	-	tig0033659:27047-27066	Msa1452060:intron	50.0%
!!	TGGCCGGCGAAATGGATTTT+GGG	-	tig0033659:27048-27067	Msa1452060:intron	50.0%
	TCCAAAGGTTGGAGTCTCCA+TGG	-	tig0033659:27088-27107	Msa1452060:CDS	50.0%
	GGAGGGAAAGGGAAGGCAAA+GGG	+	tig0033659:25191-25210	None:intergenic	55.0%
	TACCTTCCCGGAGTCTGGTA+CGG	+	tig0033659:26291-26310	None:intergenic	55.0%
	AATGGTACCTTCCCGGAGTC+TGG	+	tig0033659:26296-26315	None:intergenic	55.0%
!!	GCAAGGCAACATTGGCTCCT+AGG	+	tig0033659:26549-26568	None:intergenic	55.0%
	TGCAATGGCCGTCTGGGTTA+GGG	+	tig0033659:26868-26887	None:intergenic	55.0%
	TTGCAATGGCCGTCTGGGTT+AGG	+	tig0033659:26869-26888	None:intergenic	55.0%
	CTTGTGTTGCAATGGCCGTC+TGG	+	tig0033659:26875-26894	None:intergenic	55.0%
!!	GGCCGGCGAAATGGATTTTG+GGG	-	tig0033659:27049-27068	Msa1452060:intron	55.0%
	GGCCCCAAAATCCATTTCGC+CGG	+	tig0033659:27054-27073	None:intergenic	55.0%
	GCCATGGAGACTCCAACCTT+TGG	+	tig0033659:27092-27111	None:intergenic	55.0%
	GGAGGGAAAGGGAAGGCAAA+GGG	+	tig0033659:25191-25210	None:intergenic	55.0%
	TACCTTCCCGGAGTCTGGTA+CGG	+	tig0033659:26291-26310	None:intergenic	55.0%
	AATGGTACCTTCCCGGAGTC+TGG	+	tig0033659:26296-26315	None:intergenic	55.0%
!!	GCAAGGCAACATTGGCTCCT+AGG	+	tig0033659:26549-26568	None:intergenic	55.0%
	TGCAATGGCCGTCTGGGTTA+GGG	+	tig0033659:26868-26887	None:intergenic	55.0%
	TTGCAATGGCCGTCTGGGTT+AGG	+	tig0033659:26869-26888	None:intergenic	55.0%
	CTTGTGTTGCAATGGCCGTC+TGG	+	tig0033659:26875-26894	None:intergenic	55.0%
!!	GGCCGGCGAAATGGATTTTG+GGG	-	tig0033659:27049-27068	Msa1452060:intron	55.0%
	GGCCCCAAAATCCATTTCGC+CGG	+	tig0033659:27054-27073	None:intergenic	55.0%
	GCCATGGAGACTCCAACCTT+TGG	+	tig0033659:27092-27111	None:intergenic	55.0%
	GGGAGGGAAAGGGAAGGCAA+AGG	+	tig0033659:25192-25211	None:intergenic	60.0%
	GCTACCGCGGATCCAAACAG+AGG	-	tig0033659:25609-25628	Msa1452060:CDS	60.0%
	TACCGTACCAGACTCCGGGA+AGG	-	tig0033659:26286-26305	Msa1452060:CDS	60.0%
	GCCGAAGCCAGCAGTCAACT+TGG	+	tig0033659:26593-26612	None:intergenic	60.0%
!	CAATGGCCGTCTGGGTTAGG+GGG	+	tig0033659:26866-26885	None:intergenic	60.0%
!	GCAATGGCCGTCTGGGTTAG+GGG	+	tig0033659:26867-26886	None:intergenic	60.0%
	GGGAGGGAAAGGGAAGGCAA+AGG	+	tig0033659:25192-25211	None:intergenic	60.0%
	GCTACCGCGGATCCAAACAG+AGG	-	tig0033659:25609-25628	Msa1452060:CDS	60.0%
	TACCGTACCAGACTCCGGGA+AGG	-	tig0033659:26286-26305	Msa1452060:CDS	60.0%
	GCCGAAGCCAGCAGTCAACT+TGG	+	tig0033659:26593-26612	None:intergenic	60.0%
!	CAATGGCCGTCTGGGTTAGG+GGG	+	tig0033659:26866-26885	None:intergenic	60.0%
!	GCAATGGCCGTCTGGGTTAG+GGG	+	tig0033659:26867-26886	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0033659	gene	25088	27497	25088	ID=Msa1452060;Name=Msa1452060
tig0033659	mRNA	25088	27497	25088	ID=Msa1452060-mRNA-1;Parent=Msa1452060;Name=Msa1452060-mRNA-1;_AED=0.10;_eAED=0.10;_QI=135\|1\|1\|1\|1\|1\|5\|373\|267
tig0033659	exon	25088	25770	25088	ID=Msa1452060-mRNA-1:exon:18379;Parent=Msa1452060-mRNA-1
tig0033659	exon	25938	26164	25938	ID=Msa1452060-mRNA-1:exon:18378;Parent=Msa1452060-mRNA-1
tig0033659	exon	26278	26339	26278	ID=Msa1452060-mRNA-1:exon:18377;Parent=Msa1452060-mRNA-1
tig0033659	exon	27056	27142	27056	ID=Msa1452060-mRNA-1:exon:18376;Parent=Msa1452060-mRNA-1
tig0033659	exon	27245	27497	27245	ID=Msa1452060-mRNA-1:exon:18375;Parent=Msa1452060-mRNA-1
tig0033659	five_prime_UTR	27363	27497	27363	ID=Msa1452060-mRNA-1:five_prime_utr;Parent=Msa1452060-mRNA-1
tig0033659	CDS	27245	27362	27245	ID=Msa1452060-mRNA-1:cds;Parent=Msa1452060-mRNA-1
tig0033659	CDS	27056	27142	27056	ID=Msa1452060-mRNA-1:cds;Parent=Msa1452060-mRNA-1
tig0033659	CDS	26278	26339	26278	ID=Msa1452060-mRNA-1:cds;Parent=Msa1452060-mRNA-1
tig0033659	CDS	25938	26164	25938	ID=Msa1452060-mRNA-1:cds;Parent=Msa1452060-mRNA-1
tig0033659	CDS	25461	25770	25461	ID=Msa1452060-mRNA-1:cds;Parent=Msa1452060-mRNA-1
tig0033659	three_prime_UTR	25088	25460	25088	ID=Msa1452060-mRNA-1:three_prime_utr;Parent=Msa1452060-mRNA-1

Gene Sequence

>Msa1452060

ATGTTAACAAATCACAAAAAGGTGAAACTCAAACAACAATTTTTATTTCTTAGCATTCATTTTAGCAAAATGAGCAAGAGTCCTGGTGTATATTCTAATCTTGGCGATAAGGCCAAAGATTGTCTTTACAAGGACTATGTTCAACAATCACCTATTCACTTTCACTACCAATTCATGGATTGGAATGCAGGCTTCTCTTGTAAAGTTCAAGAAATTCTACCTGGATTCAGGACTGTTTTCAAATGTACCGTACCAGACTCCGGGAAGGTGGAACTACAGTACTTGAATAGATTCACTGGGATTACTGGATGCATTGGATTATTAGGAAACCAAGATGGAGCATATGACCCTGTTGTAAAATTATCAGGCCTTTTAGGAACAAGCATTCTGTCCCTAGGAGCCAATGTTGCCTTGCACATACCAACAAGATCGATAACCAAGTTGACTGCTGGCTTCGGCTTAAACACTGCCTTCCTTGAAGCTTCATTGACCTTGCATGACAGCTTTGACACTCTAAAAGCGACCATTTATCATGAAGTAAACCCCCTAACCCAGACGGCCATTGCAACACAAGTGAAGCATAGCTTGTCACTCAAGGAAACTGGTGTCACCATTGGTGCTCAGCATGCATTTTTTCCTGAAACATTGCTAAAGGCTCGCTTTGACAGTTCGGGCAAAGTAGGTGCTCTCATTCAACAAGGATTTTGGCAGAGATTTTTTGTAACTATGGCCGGCGAAATGGATTTTGGGGCCACCGATGATAAGATTCCAAAGGTTGGAGTCTCCATGGCTCTAAGACCCTAA

Protein sequence

>Msa1452060

MLTNHKKVKLKQQFLFLSIHFSKMSKSPGVYSNLGDKAKDCLYKDYVQQSPIHFHYQFMDWNAGFSCKVQEILPGFRTVFKCTVPDSGKVELQYLNRFTGITGCIGLLGNQDGAYDPVVKLSGLLGTSILSLGANVALHIPTRSITKLTAGFGLNTAFLEASLTLHDSFDTLKATIYHEVNPLTQTAIATQVKHSLSLKETGVTIGAQHAFFPETLLKARFDSSGKVGALIQQGFWQRFFVTMAGEMDFGATDDKIPKVGVSMALRP*