AlfalfaGEDB | Msa1299990

M. sativa cultivar zhongmu-4 / Msa1299990

KEGG: -
GO: -
PFAM: PF10160.12

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299990	sp\|Q94AH1\|CAND2_ARATH	52.459	305	102	3	23	327	39	300	4.56e-102	305

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299990	A0A072USG6	82.875	327	15	2	1	327	1	286	8.62e-180	508

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0425170	Msa1299990	0.813494	2.488455e-51	-8.615850e-47
Msa0681250	Msa1299990	0.802872	4.564216e-49	-8.615850e-47
Msa0693010	Msa1299990	-0.829141	6.077628e-55	-8.615850e-47
Msa0989360	Msa1299990	0.841977	3.424501e-58	-8.615850e-47
Msa1001180	Msa1299990	0.824619	7.317296e-54	-8.615850e-47
Msa1299990	Msa1400870	-0.818406	1.992660e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299990	MtrunA17_Chr4g0061391	82.875	327	15	2	1	327	1	286	0.0	508
Msa1299990	MtrunA17_Chr1g0191241	52.961	304	100	3	24	327	37	297	3.60e-105	309

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299990	AT3G05010.1	52.459	305	102	3	23	327	39	300	4.63e-103	305
Msa1299990	AT5G27210.1	49.508	305	111	3	23	327	36	297	2.76e-95	285

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Find 127 sgRNAs with CRISPR-Local

Find 353 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGGTAACAATCCAAAGA+AGG	0.493952	8_3:+61387513	None:intergenic
AAGAACAACCAGACCTCAAA+AGG	0.452594	8_3:+61384306	None:intergenic
AAGACCAGGCAAGGTTTAAA+AGG	0.246421	8_3:+61387495	None:intergenic
AAGACGAAATAGTGCTTTAA+TGG	0.227414	8_3:+61387873	None:intergenic
AAGCACTCATACAATCAAGT+GGG	0.623017	8_3:-61384150	Msa1299990:CDS
AAGCATTGTAAATCTGGGAA+TGG	0.497146	8_3:+61382531	None:intergenic
AAGCCACTCTCACCTTAAGA+AGG	0.534774	8_3:+61385988	None:intergenic
AAGGGTAACAATCCAAAGAA+GGG	0.578737	8_3:+61387514	None:intergenic
AATATCAATAGCCTCTGTAA+TGG	0.539878	8_3:-61387566	Msa1299990:CDS
AATCCAAAGAAGGGCATAAT+AGG	0.289361	8_3:+61387523	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGACGAAATAGTGCTTTAA+TGG	+	chr8_3:61382290-61382309	None:intergenic	30.0%
GATCGAATTCAGAAGAAAAT+GGG	+	chr8_3:61382411-61382430	None:intergenic	30.0%
ATCATCTATGTCAAGAAATG+CGG	-	chr8_3:61382452-61382471	Msa1299990:three_prime_UTR	30.0%
ACAAAACCGATGTAATAACA+AGG	+	chr8_3:61382542-61382561	None:intergenic	30.0%
AATATCAATAGCCTCTGTAA+TGG	-	chr8_3:61382594-61382613	Msa1299990:intron	30.0%
TGTAGACATACTTACAAGTA+TGG	-	chr8_3:61383121-61383140	Msa1299990:three_prime_UTR	30.0%
TGTTGAACTGTTAATCTATG+AGG	-	chr8_3:61383163-61383182	Msa1299990:three_prime_UTR	30.0%
GTTGAACTGTTAATCTATGA+GGG	-	chr8_3:61383164-61383183	Msa1299990:three_prime_UTR	30.0%
TTGAACTGTTAATCTATGAG+GGG	-	chr8_3:61383165-61383184	Msa1299990:three_prime_UTR	30.0%
AATTGAAATGTTGAAGGTTG+GGG	-	chr8_3:61383190-61383209	Msa1299990:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	61382252	61387930	61382252	ID=Msa1299990;Name=Msa1299990
chr8_3	mRNA	61382252	61387930	61382252	ID=Msa1299990-mRNA-1;Parent=Msa1299990;Name=Msa1299990-mRNA-1;_AED=0.15;_eAED=0.15;_QI=207\|0.5\|0.77\|0.88\|0.87\|0.77\|9\|979\|327
chr8_3	exon	61382252	61382576	61382252	ID=Msa1299990-mRNA-1:exon:18112;Parent=Msa1299990-mRNA-1
chr8_3	exon	61382806	61383537	61382806	ID=Msa1299990-mRNA-1:exon:18111;Parent=Msa1299990-mRNA-1
chr8_3	exon	61383636	61383717	61383636	ID=Msa1299990-mRNA-1:exon:18110;Parent=Msa1299990-mRNA-1
chr8_3	exon	61383808	61383910	61383808	ID=Msa1299990-mRNA-1:exon:18109;Parent=Msa1299990-mRNA-1
chr8_3	exon	61384060	61384222	61384060	ID=Msa1299990-mRNA-1:exon:18108;Parent=Msa1299990-mRNA-1
chr8_3	exon	61384320	61384379	61384320	ID=Msa1299990-mRNA-1:exon:18107;Parent=Msa1299990-mRNA-1
chr8_3	exon	61386001	61386198	61386001	ID=Msa1299990-mRNA-1:exon:18106;Parent=Msa1299990-mRNA-1
chr8_3	exon	61386546	61386608	61386546	ID=Msa1299990-mRNA-1:exon:18105;Parent=Msa1299990-mRNA-1
chr8_3	exon	61387487	61387930	61387487	ID=Msa1299990-mRNA-1:exon:18104;Parent=Msa1299990-mRNA-1
chr8_3	five_prime_UTR	61387724	61387930	61387724	ID=Msa1299990-mRNA-1:five_prime_utr;Parent=Msa1299990-mRNA-1
chr8_3	CDS	61387487	61387723	61387487	ID=Msa1299990-mRNA-1:cds;Parent=Msa1299990-mRNA-1
chr8_3	CDS	61386546	61386608	61386546	ID=Msa1299990-mRNA-1:cds;Parent=Msa1299990-mRNA-1
chr8_3	CDS	61386001	61386198	61386001	ID=Msa1299990-mRNA-1:cds;Parent=Msa1299990-mRNA-1
chr8_3	CDS	61384320	61384379	61384320	ID=Msa1299990-mRNA-1:cds;Parent=Msa1299990-mRNA-1
chr8_3	CDS	61384060	61384222	61384060	ID=Msa1299990-mRNA-1:cds;Parent=Msa1299990-mRNA-1
chr8_3	CDS	61383808	61383910	61383808	ID=Msa1299990-mRNA-1:cds;Parent=Msa1299990-mRNA-1
chr8_3	CDS	61383636	61383717	61383636	ID=Msa1299990-mRNA-1:cds;Parent=Msa1299990-mRNA-1
chr8_3	CDS	61383460	61383537	61383460	ID=Msa1299990-mRNA-1:cds;Parent=Msa1299990-mRNA-1
chr8_3	three_prime_UTR	61382806	61383459	61382806	ID=Msa1299990-mRNA-1:three_prime_utr;Parent=Msa1299990-mRNA-1
chr8_3	three_prime_UTR	61382252	61382576	61382252	ID=Msa1299990-mRNA-1:three_prime_utr;Parent=Msa1299990-mRNA-1

Gene Sequence

>Msa1299990

ATGTCAAGAAATGCGGAAGGTCCTGAAGCTCAAATCGATGTTGAGGATCTGAATTTGAACTGCAATGTCCACGACACGTTCCTTGTTATTACATCGGTTTTGTTTGTTTTGTATCTCGCTCTCAAAGCCAAGAAGAATATCAATAGCCTCTGTAATGGTGGTTCTTATATTATTGCTTCCTATTATGCCCTTCTTTGGATTGTTACCCTTTTAAACCTTGCCTGGTCTTTTCTTCAGAAGAGTCTTGTTCTACAACTTGATCTACCGACTGCTGTGACTGTATGTCGTGGCAAGTGCGAGGCTTGGCAGTGCTCTCCTGGAAAGGAGTTTGCATGGAATGCGTTGTCATTGTTTACGACATCAGGTATGCTGTATCTAGAGATAAGCTTGATGGCTTTCTTACTAAATGTTAACTACATGAATGCCATGGAAGCTCAAGCTCTAGCACATACTTCCATTTTATCAGGAAGTATTGTCTTGGTGGATACCCTTCTTAAGCTTGTGTTTAGATTTGTGTTGTTATTATGTCCTACTGATATCAATTTATCTCCTTTTGAGGCAATATATGTTTTTGGATTTGGGGTCCCATTGTTCAATTGCAATGTTGGAAGCACTCATACAATCAAGTGGGGTTTGTGGACAGTACATAAGTTATTGCTTGCTGCAGCATACGGCTTCATTTTATTTGAACATTTTTCTAAATGGAGAGAAAAGCTGCCTCCGAGGCCAACTTTCTACAACTATGTCGTCGTAATGTTTATATTCAGTGCAATTACTATGTTTGCTTGTGGGCTGGCGGGAATTGGTGCTGGCCTCGGCAATTGGCTGTATGATTTTACTGTACTATGTTATCACTCTTTATATCTTCCATTTCTTTATGTAACTTTTCTGGCAGATTTTTTCCAAGAGGAAGACTTTCTTTTGGACAACGCATATTATTCTGAAATGAAAGATGCTGGATTCTTTGATGCTGAGTGGGAATAA

Protein sequence

>Msa1299990

MSRNAEGPEAQIDVEDLNLNCNVHDTFLVITSVLFVLYLALKAKKNINSLCNGGSYIIASYYALLWIVTLLNLAWSFLQKSLVLQLDLPTAVTVCRGKCEAWQCSPGKEFAWNALSLFTTSGMLYLEISLMAFLLNVNYMNAMEAQALAHTSILSGSIVLVDTLLKLVFRFVLLLCPTDINLSPFEAIYVFGFGVPLFNCNVGSTHTIKWGLWTVHKLLLAAAYGFILFEHFSKWREKLPPRPTFYNYVVVMFIFSAITMFACGLAGIGAGLGNWLYDFTVLCYHSLYLPFLYVTFLADFFQEEDFLLDNAYYSEMKDAGFFDAEWE*