AlfalfaGEDB | Msa1303870

M. sativa cultivar zhongmu-4 / Msa1303870

KEGG: K13464
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003712 GO:0003714 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009791 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009966 GO:0009987 GO:0010033 GO:0010556 GO:0010558 GO:0010605 GO:0010646 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045934 GO:0048229 GO:0048367 GO:0048519 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0080134 GO:0090567 GO:0098542 GO:0140110 GO:1900067 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000022 GO:2001141
PFAM: PF06200.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303870	sp\|Q9LMA8\|TI10A_ARATH	36.328	256	106	10	1	203	1	252	1.12e-34	126

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303870	G7JS78	93.171	205	10	2	1	204	1	202	1.52e-125	362

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TFs/TRs:

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Gene ID	Type	Classification
Msa1303870	TF	Tify

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0246540	Msa1303870	0.831221	1.887085e-55	-8.615850e-47
Msa0323880	Msa1303870	0.809394	1.933960e-50	-8.615850e-47
Msa0689650	Msa1303870	0.918202	2.083799e-86	-8.615850e-47
Msa1303870	Msa1363020	0.819421	1.171859e-52	-8.615850e-47
Msa1303870	Msa1416010	0.943714	7.306911e-103	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303870	MtrunA17_Chr4g0070271	93.171	205	10	2	1	204	1	202	2.94e-129	362
Msa1303870	MtrunA17_Chr2g0301131	44.828	261	76	11	1	204	1	250	1.53e-49	161
Msa1303870	MtrunA17_Chr4g0070241	79.688	64	13	0	70	133	1	64	2.88e-29	104
Msa1303870	MtrunA17_Chr5g0399881	36.478	159	71	5	76	204	5	163	1.83e-21	87.0
Msa1303870	MtrunA17_Chr5g0399861	32.515	163	71	4	76	204	5	162	4.07e-21	85.9
Msa1303870	MtrunA17_Chr5g0399851	36.364	132	60	2	94	201	11	142	2.98e-20	83.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1303870	AT1G19180.1	36.328	256	106	10	1	203	1	252	1.14e-35	126
Msa1303870	AT1G19180.3	36.328	256	106	10	1	203	1	252	1.14e-35	126
Msa1303870	AT1G74950.1	35.745	235	84	10	7	180	1	229	2.15e-27	104
Msa1303870	AT1G74950.2	35.683	227	79	10	7	172	1	221	5.94e-24	95.1
Msa1303870	AT1G19180.2	39.098	133	52	5	99	203	55	186	1.19e-18	80.1
Msa1303870	AT1G17380.1	29.353	201	108	5	13	180	7	206	6.92e-14	68.9

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Find 49 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAATTACCAATTAGTG+AGG	0.584879	8_3:+67173565	None:intergenic
AACTACCCTTCTTCTCCTTG+AGG	0.603992	8_3:+67174237	None:intergenic
AATCAATTACCAATTAGTGA+GGG	0.634671	8_3:+67173566	None:intergenic
ACCTAACACAAGCCATGGAG+CGG	0.623220	8_3:+67172897	None:intergenic
ACGAACAGAAAGTGGGTTGA+AGG	0.513252	8_3:+67174371	None:intergenic
AGCATGCTGACTCCGCTCCA+TGG	0.562298	8_3:-67172909	Msa1303870:CDS
AGCTCCTCAACTTTCTCAGC+AGG	0.410410	8_3:+67173653	None:intergenic
AGCTTCACTTCGTCGATTCA+TGG	0.304214	8_3:-67173394	Msa1303870:CDS
ATGTCCGATGAAGTCGACAT+TGG	0.636880	8_3:+67174341	None:intergenic
ATTAGTGAGGGAGGTTGTTG+AGG	0.517348	8_3:+67173578	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTACAAGTTTGAGAAAACGT+AGG	+	chr8_3:67172833-67172852	None:intergenic	30.0%
ATTCTAAACCCCAAAACTAT+AGG	+	chr8_3:67172974-67172993	None:intergenic	30.0%
TTTCTCTATTCGGTTACAAT+TGG	-	chr8_3:67173092-67173111	Msa1303870:intron	30.0%
TTCTCTATTCGGTTACAATT+GGG	-	chr8_3:67173093-67173112	Msa1303870:intron	30.0%
AATTGCAATGACTCAAACAA+TGG	+	chr8_3:67173310-67173329	None:intergenic	30.0%
TAGATCTATCATACAGTCTT+AGG	+	chr8_3:67174355-67174374	None:intergenic	30.0%
TATCTACACGAACAGAAAGT+GGG	+	chr8_3:67172744-67172763	None:intergenic	35.0%
CAACAAACTGATAAAGTCGT+CGG	-	chr8_3:67172802-67172821	Msa1303870:three_prime_UTR	35.0%
GTTTGTGGGAAATAAATCCA+TGG	+	chr8_3:67173191-67173210	None:intergenic	35.0%
ACCGAAAAACCTTACAACTA+TGG	-	chr8_3:67173216-67173235	Msa1303870:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	67172675	67174452	67172675	ID=Msa1303870;Name=Msa1303870
chr8_3	mRNA	67172675	67174452	67172675	ID=Msa1303870-mRNA-1;Parent=Msa1303870;Name=Msa1303870-mRNA-1;_AED=0.23;_eAED=0.23;_QI=92\|1\|1\|1\|1\|1\|5\|207\|205
chr8_3	exon	67172675	67172984	67172675	ID=Msa1303870-mRNA-1:exon:20530;Parent=Msa1303870-mRNA-1
chr8_3	exon	67173378	67173435	67173378	ID=Msa1303870-mRNA-1:exon:20529;Parent=Msa1303870-mRNA-1
chr8_3	exon	67173576	67173760	67173576	ID=Msa1303870-mRNA-1:exon:20528;Parent=Msa1303870-mRNA-1
chr8_3	exon	67173873	67173975	67173873	ID=Msa1303870-mRNA-1:exon:20527;Parent=Msa1303870-mRNA-1
chr8_3	exon	67174192	67174452	67174192	ID=Msa1303870-mRNA-1:exon:20526;Parent=Msa1303870-mRNA-1
chr8_3	five_prime_UTR	67174361	67174452	67174361	ID=Msa1303870-mRNA-1:five_prime_utr;Parent=Msa1303870-mRNA-1
chr8_3	CDS	67174192	67174360	67174192	ID=Msa1303870-mRNA-1:cds;Parent=Msa1303870-mRNA-1
chr8_3	CDS	67173873	67173975	67173873	ID=Msa1303870-mRNA-1:cds;Parent=Msa1303870-mRNA-1
chr8_3	CDS	67173576	67173760	67173576	ID=Msa1303870-mRNA-1:cds;Parent=Msa1303870-mRNA-1
chr8_3	CDS	67173378	67173435	67173378	ID=Msa1303870-mRNA-1:cds;Parent=Msa1303870-mRNA-1
chr8_3	CDS	67172882	67172984	67172882	ID=Msa1303870-mRNA-1:cds;Parent=Msa1303870-mRNA-1
chr8_3	three_prime_UTR	67172675	67172881	67172675	ID=Msa1303870-mRNA-1:three_prime_utr;Parent=Msa1303870-mRNA-1

Gene Sequence

>Msa1303870

ATGTCGACTTCATCGGACATTTCTGGTTTTTCCGGCAACAAACTGATAAAGTCGTCGGAGAAACCTACGTTTTCTCAAACTTGTAATCTATTGAGCCAATACCTCAAGGAGAAGAAGGGTAGTTTCGAAGGCCTTAACCTTCACAACGCATGCACCCCTGAAACAAATGGGTCATCACCTGGTTCATCTTCTCACTCTGGAATAACCATGGATTTATTTCCCACAAACGTGACACCGAAAAACCTTACAACTATGGATTTTTTCTTTCCAAGAGTTATTAATCCTATGGTTAAAGAGCCAGAAACTGCACAATTGACAATGTTTTATAATGGACAAGTTATTGTGTTGGATGATTTTCCTGCTGAGAAAGTTGAGGAGCTGAAATCATTTGCTAGAACTCAGACTCAGCATTCAGATGTTCCTAATATGATCCCTCAACAACCTCCCTCACTAATTGATATGCCAATTGCTAGAAAAGCTTCACTTCGTCGATTCATGGAGAAGAGGAAAGATAGAGTTTCTGCCTACTCACCATATCAAAGAACCTTTCCTGACTCTGCTCCAGAAAAGCATGCTGACTCCGCTCCATGGCTTGTGTTAGGTGCTAAATCAATGTAA

Protein sequence

>Msa1303870

MSTSSDISGFSGNKLIKSSEKPTFSQTCNLLSQYLKEKKGSFEGLNLHNACTPETNGSSPGSSSHSGITMDLFPTNVTPKNLTTMDFFFPRVINPMVKEPETAQLTMFYNGQVIVLDDFPAEKVEELKSFARTQTQHSDVPNMIPQQPPSLIDMPIARKASLRRFMEKRKDRVSAYSPYQRTFPDSAPEKHADSAPWLVLGAKSM*