AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1416010


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1416010 MtrunA17_Chr4g0070271 93.171 205 10 2 1 204 1 202 2.94e-129 362
Msa1416010 MtrunA17_Chr2g0301131 44.828 261 76 11 1 204 1 250 1.53e-49 161
Msa1416010 MtrunA17_Chr4g0070241 79.688 64 13 0 70 133 1 64 2.88e-29 104
Msa1416010 MtrunA17_Chr5g0399881 36.478 159 71 5 76 204 5 163 1.83e-21 87.0
Msa1416010 MtrunA17_Chr5g0399861 32.515 163 71 4 76 204 5 162 4.07e-21 85.9
Msa1416010 MtrunA17_Chr5g0399851 36.364 132 60 2 94 201 11 142 2.98e-20 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1416010 AT1G19180.1 36.328 256 106 10 1 203 1 252 1.14e-35 126
Msa1416010 AT1G19180.3 36.328 256 106 10 1 203 1 252 1.14e-35 126
Msa1416010 AT1G74950.1 35.745 235 84 10 7 180 1 229 2.15e-27 104
Msa1416010 AT1G74950.2 35.683 227 79 10 7 172 1 221 5.94e-24 95.1
Msa1416010 AT1G19180.2 39.098 133 52 5 99 203 55 186 1.19e-18 80.1
Msa1416010 AT1G17380.1 29.353 201 108 5 13 180 7 206 6.92e-14 68.9

Find 48 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGACTTCATCGGACATTTC+TGG 0.184990 tig0024312:+1233 Msa1416010:CDS
ATTGTCAATTGTGCAGTTTC+TGG 0.207124 tig0024312:-1856 Msa1416010:intergenic
CCTTCTTCTCCTTGAGGTAT+TGG 0.218986 tig0024312:-1325 Msa1416010:intergenic
GGTTGTTGAGGGATCATATT+AGG 0.246891 tig0024312:-1979 Msa1416010:intergenic
CAACAACCTCCCTCACTAAT+TGG 0.267401 tig0024312:+1994 Msa1416010:CDS
AGCTTCACTTCGTCGATTCA+TGG 0.304214 tig0024312:+2175 Msa1416010:CDS
TCAGGAAAGGTTCTTTGATA+TGG 0.341074 tig0024312:-2623 Msa1416010:intergenic
TTTATTTACCATTTGTTTCA+GGG 0.363709 tig0024312:-1385 Msa1416010:intergenic
CGACTTTATCAGTTTGTTGC+CGG 0.376712 tig0024312:-1261 Msa1416010:intergenic
AGCTCCTCAACTTTCTCAGC+AGG 0.410410 tig0024312:-1916 Msa1416010:intergenic
TCCGCTCCATGGCTTGTGTT+AGG 0.414480 tig0024312:+2671 Msa1416010:CDS
TTACAGAGTTATTAATCCTA+TGG 0.427315 tig0024312:+1825 Msa1416010:intron
TCATGGAGAAGAGGAAAGAT+AGG 0.451183 tig0024312:+2192 Msa1416010:CDS
GTTTCTGGCTCTTTAACCAT+AGG 0.475200 tig0024312:-1841 Msa1416010:intergenic
CCAATACCTCAAGGAGAAGA+AGG 0.480824 tig0024312:+1325 Msa1416010:CDS
CAATCTACAGGGTCATCACC+TGG 0.484929 tig0024312:+1605 Msa1416010:intron
TTAGTGAGGGAGGTTGTTGA+GGG 0.495882 tig0024312:-1990 Msa1416010:intergenic
TTATTTACCATTTGTTTCAG+GGG 0.508177 tig0024312:-1384 Msa1416010:intergenic
ACGAACAGAAAGTGGGTTGA+AGG 0.513252 tig0024312:-1197 Msa1416010:intergenic
GATACCAATGTCGACTTCAT+CGG 0.515126 tig0024312:+1223 Msa1416010:exon
ATTAGTGAGGGAGGTTGTTG+AGG 0.517348 tig0024312:-1991 Msa1416010:intergenic
GCATGCACCCCTGAAACAAA+TGG 0.517892 tig0024312:+1377 Msa1416010:CDS
TAATGGACAAGTTATTGTGT+TGG 0.534161 tig0024312:+1885 Msa1416010:CDS
GAGAAGAAGGGTAGTTTCGA+AGG 0.535740 tig0024312:+1338 Msa1416010:CDS
CAGGGGTGCATGCGTTGTGA+AGG 0.536647 tig0024312:-1367 Msa1416010:intergenic
CCTGGTTCATCTTCTCACTC+TGG 0.547944 tig0024312:+1623 Msa1416010:CDS
CCAGAGTGAGAAGATGAACC+AGG 0.548191 tig0024312:-1623 Msa1416010:intergenic
AGCATGCTGACTCCGCTCCA+TGG 0.562298 tig0024312:+2660 Msa1416010:CDS
TAACTTAGTTCTATTAGCTG+AGG 0.565990 tig0024312:+2837 Msa1416010:three_prime_UTR
GTATCTACACGAACAGAAAG+TGG 0.569348 tig0024312:-1205 Msa1416010:intergenic
TGCATGCGTTGTGAAGGTTA+AGG 0.575616 tig0024312:-1361 Msa1416010:intergenic
AAATCAATTACCAATTAGTG+AGG 0.584879 tig0024312:-2004 Msa1416010:intergenic
GGTTCTTTGATATGGTGAGT+AGG 0.584950 tig0024312:-2615 Msa1416010:intergenic
TTCTGGAGCAGAGTCAGGAA+AGG 0.587295 tig0024312:-2636 Msa1416010:intergenic
GTTTGTGGGAAATAAATCCA+TGG 0.589977 tig0024312:-1651 Msa1416010:intergenic
TTAGCACCTAACACAAGCCA+TGG 0.596993 tig0024312:-2677 Msa1416010:intergenic
AACTACCCTTCTTCTCCTTG+AGG 0.603992 tig0024312:-1331 Msa1416010:intergenic
TTCTCACTCTGGAATAACCA+TGG 0.605298 tig0024312:+1634 Msa1416010:CDS
CAATTACCAATTAGTGAGGG+AGG 0.610131 tig0024312:-2000 Msa1416010:intergenic
CAATACCTCAAGGAGAAGAA+GGG 0.616382 tig0024312:+1326 Msa1416010:CDS
TTCGTCGATTCATGGAGAAG+AGG 0.618289 tig0024312:+2183 Msa1416010:CDS
ACCTAACACAAGCCATGGAG+CGG 0.623220 tig0024312:-2672 Msa1416010:intergenic
TCTATTGAGCCAATACCTCA+AGG 0.624878 tig0024312:+1316 Msa1416010:CDS
AATCAATTACCAATTAGTGA+GGG 0.634671 tig0024312:-2003 Msa1416010:intergenic
ATGTCCGATGAAGTCGACAT+TGG 0.636880 tig0024312:-1227 Msa1416010:intergenic
TTACAAGTTTGAGAAAACGT+AGG 0.650898 tig0024312:-1293 Msa1416010:intergenic
CAACAAACTGATAAAGTCGT+CGG 0.655555 tig0024312:+1265 Msa1416010:CDS
TATCTACACGAACAGAAAGT+GGG 0.660823 tig0024312:-1204 Msa1416000:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTAATTTAACTGA+TGG + tig0024312:2113-2132 Msa1416010:intron 10.0%
!!! TCTCATAATAAATCTTTTTT+CGG + tig0024312:2323-2342 Msa1416010:intron 15.0%
!! TTTATTTACCATTTGTTTCA+GGG - tig0024312:1388-1407 Msa1416010:intergenic 20.0%
!!! TTTTATTTACCATTTGTTTC+AGG - tig0024312:1389-1408 Msa1416010:intergenic 20.0%
!!! TTTTTCTTTTTTCAATCTAC+AGG + tig0024312:1593-1612 Msa1416010:intron 20.0%
!!! TTTTCTTTTTTCAATCTACA+GGG + tig0024312:1594-1613 Msa1416010:intron 20.0%
!!! CAATTGACAATGTTTTATAA+TGG + tig0024312:1868-1887 Msa1416010:CDS 20.0%
!!! ATAATAAATCTTTTTTCGGT+TGG + tig0024312:2327-2346 Msa1416010:intron 20.0%
!! GAATGACAAAAATTAATTAC+AGG - tig0024312:2887-2906 Msa1416010:intergenic 20.0%
! TTATTTACCATTTGTTTCAG+GGG - tig0024312:1387-1406 Msa1416010:intergenic 25.0%
!! AATTCAGTTCCTATAGTTTT+GGG + tig0024312:1425-1444 Msa1416010:intron 25.0%
!! TTTTTGACCAAGTTCATTTA+AGG - tig0024312:1490-1509 Msa1416010:intergenic 25.0%
! AAATGAACTTGGTCAAAAAA+TGG + tig0024312:1491-1510 Msa1416010:intron 25.0%
!!! TTCCCTTATTTTTCTCTATT+CGG + tig0024312:1545-1564 Msa1416010:intron 25.0%
! AACCGAATAGAGAAAAATAA+GGG - tig0024312:1550-1569 Msa1416010:intergenic 25.0%
! TAACCGAATAGAGAAAAATA+AGG - tig0024312:1551-1570 Msa1416010:intergenic 25.0%
! AGAAAAAATCCATAGTTGTA+AGG - tig0024312:1691-1710 Msa1416010:intergenic 25.0%
! TTACAGAGTTATTAATCCTA+TGG + tig0024312:1825-1844 Msa1416010:intron 25.0%
! AATCAATTACCAATTAGTGA+GGG - tig0024312:2006-2025 Msa1416010:intergenic 25.0%
! AAATCAATTACCAATTAGTG+AGG - tig0024312:2007-2026 Msa1416010:intergenic 25.0%
!! TTGTGATTTGAAACAAATAC+AGG + tig0024312:2052-2071 Msa1416010:intron 25.0%
!! CAAATACAGGAAATTGTTTT+TGG + tig0024312:2065-2084 Msa1416010:intron 25.0%
!!! ATTTAATTTGTTTGAGAGAG+AGG - tig0024312:2222-2241 Msa1416010:intergenic 25.0%
! AATCCAAAATCCAAATTAGT+TGG - tig0024312:2355-2374 Msa1416010:intergenic 25.0%
!!! TTGAACATTTTTTGAGCTTT+TGG + tig0024312:2373-2392 Msa1416010:intron 25.0%
! CTTGATAAGTAAATAACAAG+AGG - tig0024312:2519-2538 Msa1416010:intergenic 25.0%
TTACAAGTTTGAGAAAACGT+AGG - tig0024312:1296-1315 Msa1416010:intergenic 30.0%
! CAATTCAGTTCCTATAGTTT+TGG + tig0024312:1424-1443 Msa1416010:intron 30.0%
!! ATTCAGTTCCTATAGTTTTG+GGG + tig0024312:1426-1445 Msa1416010:intron 30.0%
ATTCTAAACCCCAAAACTAT+AGG - tig0024312:1437-1456 Msa1416010:intergenic 30.0%
TAGTCTTCCTTAAATGAACT+TGG + tig0024312:1480-1499 Msa1416010:intron 30.0%
TTTCTCTATTCGGTTACAAT+TGG + tig0024312:1555-1574 Msa1416010:intron 30.0%
TTCTCTATTCGGTTACAATT+GGG + tig0024312:1556-1575 Msa1416010:intron 30.0%
! TCCATAGTTGTAAGGTTTTT+CGG - tig0024312:1683-1702 Msa1416010:intergenic 30.0%
!!! CTATGGATTTTTTCTTTCCA+AGG + tig0024312:1696-1715 Msa1416010:CDS 30.0%
AATTGCAATGACTCAAACAA+TGG - tig0024312:1773-1792 Msa1416010:intergenic 30.0%
! TAATGGACAAGTTATTGTGT+TGG + tig0024312:1885-1904 Msa1416010:CDS 30.0%
!!! AGGAAATTGTTTTTGGTATC+AGG + tig0024312:2072-2091 Msa1416010:intron 30.0%
!!! GGAAATTGTTTTTGGTATCA+GGG + tig0024312:2073-2092 Msa1416010:intron 30.0%
! GTACCAACTAATTTGGATTT+TGG + tig0024312:2349-2368 Msa1416010:intron 30.0%
!! TAACTTAGTTCTATTAGCTG+AGG + tig0024312:2837-2856 Msa1416010:three_prime_UTR 30.0%
TATCTACACGAACAGAAAGT+GGG - tig0024312:1207-1226 None:intergenic 35.0%
CAACAAACTGATAAAGTCGT+CGG + tig0024312:1265-1284 Msa1416010:CDS 35.0%
GTTTGTGGGAAATAAATCCA+TGG - tig0024312:1654-1673 Msa1416010:intergenic 35.0%
ACCGAAAAACCTTACAACTA+TGG + tig0024312:1679-1698 Msa1416010:CDS 35.0%
! AAAAGCACAAAAACTCACCT+TGG - tig0024312:1716-1735 Msa1416010:intergenic 35.0%
ATTGTCAATTGTGCAGTTTC+TGG - tig0024312:1859-1878 Msa1416010:intergenic 35.0%
!! AGTGAAGCTTTTCTAGCAAT+TGG - tig0024312:2164-2183 Msa1416010:intergenic 35.0%
ATGCATGCATAGCATAAATC+TGG + tig0024312:2398-2417 Msa1416010:intron 35.0%
ATTTCCAGTCACACAAAGAA+AGG - tig0024312:2424-2443 Msa1416010:intergenic 35.0%
TCAGGAAAGGTTCTTTGATA+TGG - tig0024312:2626-2645 Msa1416010:intergenic 35.0%
GTATCTACACGAACAGAAAG+TGG - tig0024312:1208-1227 None:intergenic 40.0%
!! GATACCAATGTCGACTTCAT+CGG + tig0024312:1223-1242 Msa1416010:exon 40.0%
!!! TCGGACATTTCTGGTTTTTC+CGG + tig0024312:1242-1261 Msa1416010:CDS 40.0%
!! CGACTTTATCAGTTTGTTGC+CGG - tig0024312:1264-1283 Msa1416010:intergenic 40.0%
TCTATTGAGCCAATACCTCA+AGG + tig0024312:1316-1335 Msa1416010:CDS 40.0%
CAATACCTCAAGGAGAAGAA+GGG + tig0024312:1326-1345 Msa1416010:CDS 40.0%
!! TTCTCACTCTGGAATAACCA+TGG + tig0024312:1634-1653 Msa1416010:CDS 40.0%
!!! TTTTTCGGTGTCACGTTTGT+GGG - tig0024312:1668-1687 Msa1416010:intergenic 40.0%
!! GTTTCTGGCTCTTTAACCAT+AGG - tig0024312:1844-1863 Msa1416010:intergenic 40.0%
! TTTTCCTGCTGAGAAAGTTG+AGG + tig0024312:1912-1931 Msa1416010:CDS 40.0%
GGTTGTTGAGGGATCATATT+AGG - tig0024312:1982-2001 Msa1416010:intergenic 40.0%
CAATTACCAATTAGTGAGGG+AGG - tig0024312:2003-2022 Msa1416010:intergenic 40.0%
TCATGGAGAAGAGGAAAGAT+AGG + tig0024312:2192-2211 Msa1416010:CDS 40.0%
! TCGGTTGGTACCAACTAATT+TGG + tig0024312:2342-2361 Msa1416010:intron 40.0%
AGGCAAAGCAATTTGCACAA+TGG - tig0024312:2499-2518 Msa1416010:intergenic 40.0%
GGTTCTTTGATATGGTGAGT+AGG - tig0024312:2618-2637 Msa1416010:intergenic 40.0%
!! ACGAACAGAAAGTGGGTTGA+AGG - tig0024312:1200-1219 None:intergenic 45.0%
ATGTCCGATGAAGTCGACAT+TGG - tig0024312:1230-1249 Msa1416010:intergenic 45.0%
TCGACTTCATCGGACATTTC+TGG + tig0024312:1233-1252 Msa1416010:CDS 45.0%
CCTTCTTCTCCTTGAGGTAT+TGG - tig0024312:1328-1347 Msa1416010:intergenic 45.0%
CCAATACCTCAAGGAGAAGA+AGG + tig0024312:1325-1344 Msa1416010:CDS 45.0%
AACTACCCTTCTTCTCCTTG+AGG - tig0024312:1334-1353 Msa1416010:intergenic 45.0%
GAGAAGAAGGGTAGTTTCGA+AGG + tig0024312:1338-1357 Msa1416010:CDS 45.0%
TGCATGCGTTGTGAAGGTTA+AGG - tig0024312:1364-1383 Msa1416010:intergenic 45.0%
!!! GTTTTTCGGTGTCACGTTTG+TGG - tig0024312:1669-1688 Msa1416010:intergenic 45.0%
TTAGTGAGGGAGGTTGTTGA+GGG - tig0024312:1993-2012 Msa1416010:intergenic 45.0%
ATTAGTGAGGGAGGTTGTTG+AGG - tig0024312:1994-2013 Msa1416010:intergenic 45.0%
CAACAACCTCCCTCACTAAT+TGG + tig0024312:1994-2013 Msa1416010:CDS 45.0%
!! AGCTTCACTTCGTCGATTCA+TGG + tig0024312:2175-2194 Msa1416010:CDS 45.0%
!! TTCGTCGATTCATGGAGAAG+AGG + tig0024312:2183-2202 Msa1416010:CDS 45.0%
TTAGCACCTAACACAAGCCA+TGG - tig0024312:2680-2699 Msa1416010:intergenic 45.0%
!! AATAACAATATTATTAAATT+AGG - tig0024312:2567-2586 Msa1416010:intergenic 5.0%
!! TAAAAATAATAAACAAAAAA+AGG - tig0024312:2756-2775 Msa1416010:intergenic 5.0%
!!! TTTATTATTTTTATTTTGAT+CGG + tig0024312:2763-2782 Msa1416010:three_prime_UTR 5.0%
GCATGCACCCCTGAAACAAA+TGG + tig0024312:1377-1396 Msa1416010:CDS 50.0%
CAATCTACAGGGTCATCACC+TGG + tig0024312:1605-1624 Msa1416010:intron 50.0%
CCAGAGTGAGAAGATGAACC+AGG - tig0024312:1626-1645 Msa1416010:intergenic 50.0%
CCTGGTTCATCTTCTCACTC+TGG + tig0024312:1623-1642 Msa1416010:CDS 50.0%
AGCTCCTCAACTTTCTCAGC+AGG - tig0024312:1919-1938 Msa1416010:intergenic 50.0%
! CTGGCCTTTCTTTGTGTGAC+TGG + tig0024312:2417-2436 Msa1416010:intron 50.0%
!! TTCTGGAGCAGAGTCAGGAA+AGG - tig0024312:2639-2658 Msa1416010:intergenic 50.0%
!!! TGCTTTTCTGGAGCAGAGTC+AGG - tig0024312:2644-2663 Msa1416010:intergenic 50.0%
ACCTAACACAAGCCATGGAG+CGG - tig0024312:2675-2694 Msa1416010:intergenic 50.0%
! GCGGAGTCAGCATGCTTTTC+TGG - tig0024312:2656-2675 Msa1416010:intergenic 55.0%
! TCCGCTCCATGGCTTGTGTT+AGG + tig0024312:2671-2690 Msa1416010:CDS 55.0%
! CAGGGGTGCATGCGTTGTGA+AGG - tig0024312:1370-1389 Msa1416010:intergenic 60.0%
!! AGCATGCTGACTCCGCTCCA+TGG + tig0024312:2660-2679 Msa1416010:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0024312 gene 1138 2916 1138 ID=Msa1416010;Name=Msa1416010
tig0024312 mRNA 1138 2916 1138 ID=Msa1416010-mRNA-1;Parent=Msa1416010;Name=Msa1416010-mRNA-1;_AED=0.10;_eAED=0.10;_QI=92|1|1|1|1|1|5|207|205
tig0024312 exon 1138 1398 1138 ID=Msa1416010-mRNA-1:exon:22724;Parent=Msa1416010-mRNA-1
tig0024312 exon 1615 1717 1615 ID=Msa1416010-mRNA-1:exon:22725;Parent=Msa1416010-mRNA-1
tig0024312 exon 1831 2015 1831 ID=Msa1416010-mRNA-1:exon:22726;Parent=Msa1416010-mRNA-1
tig0024312 exon 2156 2213 2156 ID=Msa1416010-mRNA-1:exon:22727;Parent=Msa1416010-mRNA-1
tig0024312 exon 2607 2916 2607 ID=Msa1416010-mRNA-1:exon:22728;Parent=Msa1416010-mRNA-1
tig0024312 five_prime_UTR 1138 1229 1138 ID=Msa1416010-mRNA-1:five_prime_utr;Parent=Msa1416010-mRNA-1
tig0024312 CDS 1230 1398 1230 ID=Msa1416010-mRNA-1:cds;Parent=Msa1416010-mRNA-1
tig0024312 CDS 1615 1717 1615 ID=Msa1416010-mRNA-1:cds;Parent=Msa1416010-mRNA-1
tig0024312 CDS 1831 2015 1831 ID=Msa1416010-mRNA-1:cds;Parent=Msa1416010-mRNA-1
tig0024312 CDS 2156 2213 2156 ID=Msa1416010-mRNA-1:cds;Parent=Msa1416010-mRNA-1
tig0024312 CDS 2607 2709 2607 ID=Msa1416010-mRNA-1:cds;Parent=Msa1416010-mRNA-1
tig0024312 three_prime_UTR 2710 2916 2710 ID=Msa1416010-mRNA-1:three_prime_utr;Parent=Msa1416010-mRNA-1
Gene Sequence

>Msa1416010

ATGTCGACTTCATCGGACATTTCTGGTTTTTCCGGCAACAAACTGATAAAGTCGTCGGAGAAACCTACGTTTTCTCAAACTTGTAATCTATTGAGCCAATACCTCAAGGAGAAGAAGGGTAGTTTCGAAGGCCTTAACCTTCACAACGCATGCACCCCTGAAACAAATGGGTCATCACCTGGTTCATCTTCTCACTCTGGAATAACCATGGATTTATTTCCCACAAACGTGACACCGAAAAACCTTACAACTATGGATTTTTTCTTTCCAAGAGTTATTAATCCTATGGTTAAAGAGCCAGAAACTGCACAATTGACAATGTTTTATAATGGACAAGTTATTGTGTTGGATGATTTTCCTGCTGAGAAAGTTGAGGAGCTGAAATCATTTGCTAGAACTCAGACTCAGCATTCAGATGTTCCTAATATGATCCCTCAACAACCTCCCTCACTAATTGATATGCCAATTGCTAGAAAAGCTTCACTTCGTCGATTCATGGAGAAGAGGAAAGATAGAGTTTCTGCCTACTCACCATATCAAAGAACCTTTCCTGACTCTGCTCCAGAAAAGCATGCTGACTCCGCTCCATGGCTTGTGTTAGGTGCTAAATCAATGTAA

Protein sequence

>Msa1416010

MSTSSDISGFSGNKLIKSSEKPTFSQTCNLLSQYLKEKKGSFEGLNLHNACTPETNGSSPGSSSHSGITMDLFPTNVTPKNLTTMDFFFPRVINPMVKEPETAQLTMFYNGQVIVLDDFPAEKVEELKSFARTQTQHSDVPNMIPQQPPSLIDMPIARKASLRRFMEKRKDRVSAYSPYQRTFPDSAPEKHADSAPWLVLGAKSM*