AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1363020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1363020 MtrunA17_Chr4g0070271 93.171 205 10 2 1 204 1 202 2.94e-129 362
Msa1363020 MtrunA17_Chr2g0301131 44.828 261 76 11 1 204 1 250 1.53e-49 161
Msa1363020 MtrunA17_Chr4g0070241 79.688 64 13 0 70 133 1 64 2.88e-29 104
Msa1363020 MtrunA17_Chr5g0399881 36.478 159 71 5 76 204 5 163 1.83e-21 87.0
Msa1363020 MtrunA17_Chr5g0399861 32.515 163 71 4 76 204 5 162 4.07e-21 85.9
Msa1363020 MtrunA17_Chr5g0399851 36.364 132 60 2 94 201 11 142 2.98e-20 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1363020 AT1G19180.1 36.328 256 106 10 1 203 1 252 1.14e-35 126
Msa1363020 AT1G19180.3 36.328 256 106 10 1 203 1 252 1.14e-35 126
Msa1363020 AT1G74950.1 35.745 235 84 10 7 180 1 229 2.15e-27 104
Msa1363020 AT1G74950.2 35.683 227 79 10 7 172 1 221 5.94e-24 95.1
Msa1363020 AT1G19180.2 39.098 133 52 5 99 203 55 186 1.19e-18 80.1
Msa1363020 AT1G17380.1 29.353 201 108 5 13 180 7 206 6.92e-14 68.9

Find 49 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGACTTCATCGGACATTTC+TGG 0.184990 tig0018025:+57311 Msa1363020:CDS
ATTGTCAATTGTGCAGTTTC+TGG 0.207124 tig0018025:-57934 None:intergenic
CCTTCTTCTCCTTGAGGTAT+TGG 0.223124 tig0018025:-57403 None:intergenic
GGTTGTTGAGGGATCATATT+AGG 0.246891 tig0018025:-58057 None:intergenic
CAACAACCTCCCTCACTAAT+TGG 0.267401 tig0018025:+58072 Msa1363020:CDS
TAGATCTATCATACAGTCTT+AGG 0.298138 tig0018025:-58894 None:intergenic
AGCTTCACTTCGTCGATTCA+TGG 0.304214 tig0018025:+58253 Msa1363020:CDS
TCAGGAAAGGTTCTTTGATA+TGG 0.341074 tig0018025:-58701 None:intergenic
TTTATTTACCATTTGTTTCA+GGG 0.363709 tig0018025:-57463 None:intergenic
CGACTTTATCAGTTTGTTGC+CGG 0.376712 tig0018025:-57339 None:intergenic
AGCTCCTCAACTTTCTCAGC+AGG 0.410410 tig0018025:-57994 None:intergenic
TCCGCTCCATGGCTTGTGTT+AGG 0.414480 tig0018025:+58749 Msa1363020:CDS
TTACAGAGTTATTAATCCTA+TGG 0.427315 tig0018025:+57903 Msa1363020:intron
TCATGGAGAAGAGGAAAGAT+AGG 0.451183 tig0018025:+58270 Msa1363020:CDS
GTTTCTGGCTCTTTAACCAT+AGG 0.475200 tig0018025:-57919 None:intergenic
CAATCTACAGGGTCATCACC+TGG 0.484929 tig0018025:+57683 Msa1363020:intron
CCAATACCTCAAGGAGAAGA+AGG 0.485641 tig0018025:+57403 Msa1363020:CDS
TTAGTGAGGGAGGTTGTTGA+GGG 0.495882 tig0018025:-58068 None:intergenic
GAGAAGAAGGGCAGTTTCGA+AGG 0.507391 tig0018025:+57416 Msa1363020:CDS
TTATTTACCATTTGTTTCAG+GGG 0.508177 tig0018025:-57462 None:intergenic
ACGAACAGAAAGTGGGTTGA+AGG 0.513252 tig0018025:-57275 None:intergenic
GATACCAATGTCGACTTCAT+CGG 0.515126 tig0018025:+57301 Msa1363020:exon
ATTAGTGAGGGAGGTTGTTG+AGG 0.517348 tig0018025:-58069 None:intergenic
GCATGCACCCCTGAAACAAA+TGG 0.517892 tig0018025:+57455 Msa1363020:CDS
TAATGGACAAGTTATTGTGT+TGG 0.534161 tig0018025:+57963 Msa1363020:CDS
CAGGGGTGCATGCGTTGTGA+AGG 0.536647 tig0018025:-57445 None:intergenic
CCTGGTTCATCTTCTCACTC+TGG 0.547944 tig0018025:+57701 Msa1363020:CDS
CCAGAGTGAGAAGATGAACC+AGG 0.548191 tig0018025:-57701 None:intergenic
AGCATGCTGACTCCGCTCCA+TGG 0.562298 tig0018025:+58738 Msa1363020:CDS
TAACTTAGTTCTATTAGCTG+AGG 0.565990 tig0018025:+58915 Msa1363020:three_prime_UTR
AACTGCCCTTCTTCTCCTTG+AGG 0.566368 tig0018025:-57409 None:intergenic
GTATCTACACGAACAGAAAG+TGG 0.569348 tig0018025:-57283 None:intergenic
TGCATGCGTTGTGAAGGTTA+AGG 0.575616 tig0018025:-57439 None:intergenic
AAATCAATTACCAATTAGTG+AGG 0.584879 tig0018025:-58082 None:intergenic
GGTTCTTTGATATGGTGAGT+AGG 0.584950 tig0018025:-58693 None:intergenic
TTCTGGAGCAGAGTCAGGAA+AGG 0.587295 tig0018025:-58714 None:intergenic
GTTTGTGGGAAATAAATCCA+TGG 0.589977 tig0018025:-57729 None:intergenic
TTAGCACCTAACACAAGCCA+TGG 0.596993 tig0018025:-58755 None:intergenic
TTCTCACTCTGGAATAACCA+TGG 0.605298 tig0018025:+57712 Msa1363020:CDS
CAATTACCAATTAGTGAGGG+AGG 0.610131 tig0018025:-58078 None:intergenic
TTCGTCGATTCATGGAGAAG+AGG 0.618289 tig0018025:+58261 Msa1363020:CDS
CAATACCTCAAGGAGAAGAA+GGG 0.622660 tig0018025:+57404 Msa1363020:CDS
ACCTAACACAAGCCATGGAG+CGG 0.623220 tig0018025:-58750 None:intergenic
TCTATTGAGCCAATACCTCA+AGG 0.624878 tig0018025:+57394 Msa1363020:CDS
AATCAATTACCAATTAGTGA+GGG 0.634671 tig0018025:-58081 None:intergenic
ATGTCCGATGAAGTCGACAT+TGG 0.636880 tig0018025:-57305 None:intergenic
TTACAAGTTTGAGAAAACGT+AGG 0.650898 tig0018025:-57371 None:intergenic
CAACAAACTGATAAAGTCGT+CGG 0.655555 tig0018025:+57343 Msa1363020:CDS
TATCTACACGAACAGAAAGT+GGG 0.660823 tig0018025:-57282 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTAATTTAACTGA+TGG + tig0018025:58191-58210 Msa1363020:intron 10.0%
!!! TCTCATAATAAATCTTTTTT+CGG + tig0018025:58401-58420 Msa1363020:intron 15.0%
!! TTTATTTACCATTTGTTTCA+GGG - tig0018025:57466-57485 None:intergenic 20.0%
!!! TTTTATTTACCATTTGTTTC+AGG - tig0018025:57467-57486 None:intergenic 20.0%
!!! TTTTTCTTTTTTCAATCTAC+AGG + tig0018025:57671-57690 Msa1363020:intron 20.0%
!!! TTTTCTTTTTTCAATCTACA+GGG + tig0018025:57672-57691 Msa1363020:intron 20.0%
!!! CAATTGACAATGTTTTATAA+TGG + tig0018025:57946-57965 Msa1363020:CDS 20.0%
!!! ATAATAAATCTTTTTTCGGT+TGG + tig0018025:58405-58424 Msa1363020:intron 20.0%
!! GAATGACAAAAATTAATTAC+AGG - tig0018025:58965-58984 None:intergenic 20.0%
! TTATTTACCATTTGTTTCAG+GGG - tig0018025:57465-57484 None:intergenic 25.0%
!! AATTCAGTTCCTATAGTTTT+GGG + tig0018025:57503-57522 Msa1363020:intron 25.0%
!! TTTTTGACCAAGTTCATTTA+AGG - tig0018025:57568-57587 None:intergenic 25.0%
! AAATGAACTTGGTCAAAAAA+TGG + tig0018025:57569-57588 Msa1363020:intron 25.0%
!!! TTCCCTTATTTTTCTCTATT+CGG + tig0018025:57623-57642 Msa1363020:intron 25.0%
! AACCGAATAGAGAAAAATAA+GGG - tig0018025:57628-57647 None:intergenic 25.0%
! TAACCGAATAGAGAAAAATA+AGG - tig0018025:57629-57648 None:intergenic 25.0%
! AGAAAAAATCCATAGTTGTA+AGG - tig0018025:57769-57788 None:intergenic 25.0%
! TTACAGAGTTATTAATCCTA+TGG + tig0018025:57903-57922 Msa1363020:intron 25.0%
! AATCAATTACCAATTAGTGA+GGG - tig0018025:58084-58103 None:intergenic 25.0%
! AAATCAATTACCAATTAGTG+AGG - tig0018025:58085-58104 None:intergenic 25.0%
!! TTGTGATTTGAAACAAATAC+AGG + tig0018025:58130-58149 Msa1363020:intron 25.0%
!! CAAATACAGGAAATTGTTTT+TGG + tig0018025:58143-58162 Msa1363020:intron 25.0%
!!! ATTTAATTTGTTTGAGAGAG+AGG - tig0018025:58300-58319 None:intergenic 25.0%
! AATCCAAAATCCAAATTAGT+TGG - tig0018025:58433-58452 None:intergenic 25.0%
!!! TTGAACATTTTTTGAGCTTT+TGG + tig0018025:58451-58470 Msa1363020:intron 25.0%
! CTTGATAAGTAAATAACAAG+AGG - tig0018025:58597-58616 None:intergenic 25.0%
TTACAAGTTTGAGAAAACGT+AGG - tig0018025:57374-57393 None:intergenic 30.0%
! CAATTCAGTTCCTATAGTTT+TGG + tig0018025:57502-57521 Msa1363020:intron 30.0%
!! ATTCAGTTCCTATAGTTTTG+GGG + tig0018025:57504-57523 Msa1363020:intron 30.0%
ATTCTAAACCCCAAAACTAT+AGG - tig0018025:57515-57534 None:intergenic 30.0%
TAGTCTTCCTTAAATGAACT+TGG + tig0018025:57558-57577 Msa1363020:intron 30.0%
TTTCTCTATTCGGTTACAAT+TGG + tig0018025:57633-57652 Msa1363020:intron 30.0%
TTCTCTATTCGGTTACAATT+GGG + tig0018025:57634-57653 Msa1363020:intron 30.0%
! TCCATAGTTGTAAGGTTTTT+CGG - tig0018025:57761-57780 None:intergenic 30.0%
!!! CTATGGATTTTTTCTTTCCA+AGG + tig0018025:57774-57793 Msa1363020:CDS 30.0%
AATTGCAATGACTCAAACAA+TGG - tig0018025:57851-57870 None:intergenic 30.0%
! TAATGGACAAGTTATTGTGT+TGG + tig0018025:57963-57982 Msa1363020:CDS 30.0%
!!! AGGAAATTGTTTTTGGTATC+AGG + tig0018025:58150-58169 Msa1363020:intron 30.0%
!!! GGAAATTGTTTTTGGTATCA+GGG + tig0018025:58151-58170 Msa1363020:intron 30.0%
! GTACCAACTAATTTGGATTT+TGG + tig0018025:58427-58446 Msa1363020:intron 30.0%
TAGATCTATCATACAGTCTT+AGG - tig0018025:58897-58916 None:intergenic 30.0%
!! TAACTTAGTTCTATTAGCTG+AGG + tig0018025:58915-58934 Msa1363020:three_prime_UTR 30.0%
TATCTACACGAACAGAAAGT+GGG - tig0018025:57285-57304 None:intergenic 35.0%
CAACAAACTGATAAAGTCGT+CGG + tig0018025:57343-57362 Msa1363020:CDS 35.0%
GTTTGTGGGAAATAAATCCA+TGG - tig0018025:57732-57751 None:intergenic 35.0%
ACCGAAAAACCTTACAACTA+TGG + tig0018025:57757-57776 Msa1363020:CDS 35.0%
! AAAAGCACAAAAACTCACCT+TGG - tig0018025:57794-57813 None:intergenic 35.0%
ATTGTCAATTGTGCAGTTTC+TGG - tig0018025:57937-57956 None:intergenic 35.0%
!! AGTGAAGCTTTTCTAGCAAT+TGG - tig0018025:58242-58261 None:intergenic 35.0%
ATGCATGCATAGCATAAATC+TGG + tig0018025:58476-58495 Msa1363020:intron 35.0%
ATTTCCAGTCACACAAAGAA+AGG - tig0018025:58502-58521 None:intergenic 35.0%
TCAGGAAAGGTTCTTTGATA+TGG - tig0018025:58704-58723 None:intergenic 35.0%
GTATCTACACGAACAGAAAG+TGG - tig0018025:57286-57305 None:intergenic 40.0%
!! GATACCAATGTCGACTTCAT+CGG + tig0018025:57301-57320 Msa1363020:exon 40.0%
!!! TCGGACATTTCTGGTTTTTC+CGG + tig0018025:57320-57339 Msa1363020:CDS 40.0%
!! CGACTTTATCAGTTTGTTGC+CGG - tig0018025:57342-57361 None:intergenic 40.0%
TCTATTGAGCCAATACCTCA+AGG + tig0018025:57394-57413 Msa1363020:CDS 40.0%
CAATACCTCAAGGAGAAGAA+GGG + tig0018025:57404-57423 Msa1363020:CDS 40.0%
!! TTCTCACTCTGGAATAACCA+TGG + tig0018025:57712-57731 Msa1363020:CDS 40.0%
!!! TTTTTCGGTGTCACGTTTGT+GGG - tig0018025:57746-57765 None:intergenic 40.0%
!! GTTTCTGGCTCTTTAACCAT+AGG - tig0018025:57922-57941 None:intergenic 40.0%
! TTTTCCTGCTGAGAAAGTTG+AGG + tig0018025:57990-58009 Msa1363020:CDS 40.0%
GGTTGTTGAGGGATCATATT+AGG - tig0018025:58060-58079 None:intergenic 40.0%
CAATTACCAATTAGTGAGGG+AGG - tig0018025:58081-58100 None:intergenic 40.0%
TCATGGAGAAGAGGAAAGAT+AGG + tig0018025:58270-58289 Msa1363020:CDS 40.0%
! TCGGTTGGTACCAACTAATT+TGG + tig0018025:58420-58439 Msa1363020:intron 40.0%
AGGCAAAGCAATTTGCACAA+TGG - tig0018025:58577-58596 None:intergenic 40.0%
GGTTCTTTGATATGGTGAGT+AGG - tig0018025:58696-58715 None:intergenic 40.0%
!! ACGAACAGAAAGTGGGTTGA+AGG - tig0018025:57278-57297 None:intergenic 45.0%
ATGTCCGATGAAGTCGACAT+TGG - tig0018025:57308-57327 None:intergenic 45.0%
TCGACTTCATCGGACATTTC+TGG + tig0018025:57311-57330 Msa1363020:CDS 45.0%
CCTTCTTCTCCTTGAGGTAT+TGG - tig0018025:57406-57425 None:intergenic 45.0%
CCAATACCTCAAGGAGAAGA+AGG + tig0018025:57403-57422 Msa1363020:CDS 45.0%
TGCATGCGTTGTGAAGGTTA+AGG - tig0018025:57442-57461 None:intergenic 45.0%
!!! GTTTTTCGGTGTCACGTTTG+TGG - tig0018025:57747-57766 None:intergenic 45.0%
TTAGTGAGGGAGGTTGTTGA+GGG - tig0018025:58071-58090 None:intergenic 45.0%
ATTAGTGAGGGAGGTTGTTG+AGG - tig0018025:58072-58091 None:intergenic 45.0%
CAACAACCTCCCTCACTAAT+TGG + tig0018025:58072-58091 Msa1363020:CDS 45.0%
!! AGCTTCACTTCGTCGATTCA+TGG + tig0018025:58253-58272 Msa1363020:CDS 45.0%
!! TTCGTCGATTCATGGAGAAG+AGG + tig0018025:58261-58280 Msa1363020:CDS 45.0%
TTAGCACCTAACACAAGCCA+TGG - tig0018025:58758-58777 None:intergenic 45.0%
!! AATAACAATATTATTAAATT+AGG - tig0018025:58645-58664 None:intergenic 5.0%
!! TAAAAATAATAAACAAAAAA+AGG - tig0018025:58834-58853 None:intergenic 5.0%
!!! TTTATTATTTTTATTTTGAT+CGG + tig0018025:58841-58860 Msa1363020:three_prime_UTR 5.0%
AACTGCCCTTCTTCTCCTTG+AGG - tig0018025:57412-57431 None:intergenic 50.0%
GAGAAGAAGGGCAGTTTCGA+AGG + tig0018025:57416-57435 Msa1363020:CDS 50.0%
GCATGCACCCCTGAAACAAA+TGG + tig0018025:57455-57474 Msa1363020:CDS 50.0%
CAATCTACAGGGTCATCACC+TGG + tig0018025:57683-57702 Msa1363020:intron 50.0%
CCAGAGTGAGAAGATGAACC+AGG - tig0018025:57704-57723 None:intergenic 50.0%
CCTGGTTCATCTTCTCACTC+TGG + tig0018025:57701-57720 Msa1363020:CDS 50.0%
AGCTCCTCAACTTTCTCAGC+AGG - tig0018025:57997-58016 None:intergenic 50.0%
! CTGGCCTTTCTTTGTGTGAC+TGG + tig0018025:58495-58514 Msa1363020:intron 50.0%
!! TTCTGGAGCAGAGTCAGGAA+AGG - tig0018025:58717-58736 None:intergenic 50.0%
!!! TGCTTTTCTGGAGCAGAGTC+AGG - tig0018025:58722-58741 None:intergenic 50.0%
ACCTAACACAAGCCATGGAG+CGG - tig0018025:58753-58772 None:intergenic 50.0%
! GCGGAGTCAGCATGCTTTTC+TGG - tig0018025:58734-58753 None:intergenic 55.0%
! TCCGCTCCATGGCTTGTGTT+AGG + tig0018025:58749-58768 Msa1363020:CDS 55.0%
! CAGGGGTGCATGCGTTGTGA+AGG - tig0018025:57448-57467 None:intergenic 60.0%
!! AGCATGCTGACTCCGCTCCA+TGG + tig0018025:58738-58757 Msa1363020:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0018025 gene 57216 58994 57216 ID=Msa1363020;Name=Msa1363020
tig0018025 mRNA 57216 58994 57216 ID=Msa1363020-mRNA-1;Parent=Msa1363020;Name=Msa1363020-mRNA-1;_AED=0.10;_eAED=0.10;_QI=92|1|1|1|1|1|5|207|205
tig0018025 exon 57216 57476 57216 ID=Msa1363020-mRNA-1:exon:13337;Parent=Msa1363020-mRNA-1
tig0018025 exon 57693 57795 57693 ID=Msa1363020-mRNA-1:exon:13338;Parent=Msa1363020-mRNA-1
tig0018025 exon 57909 58093 57909 ID=Msa1363020-mRNA-1:exon:13339;Parent=Msa1363020-mRNA-1
tig0018025 exon 58234 58291 58234 ID=Msa1363020-mRNA-1:exon:13340;Parent=Msa1363020-mRNA-1
tig0018025 exon 58685 58994 58685 ID=Msa1363020-mRNA-1:exon:13341;Parent=Msa1363020-mRNA-1
tig0018025 five_prime_UTR 57216 57307 57216 ID=Msa1363020-mRNA-1:five_prime_utr;Parent=Msa1363020-mRNA-1
tig0018025 CDS 57308 57476 57308 ID=Msa1363020-mRNA-1:cds;Parent=Msa1363020-mRNA-1
tig0018025 CDS 57693 57795 57693 ID=Msa1363020-mRNA-1:cds;Parent=Msa1363020-mRNA-1
tig0018025 CDS 57909 58093 57909 ID=Msa1363020-mRNA-1:cds;Parent=Msa1363020-mRNA-1
tig0018025 CDS 58234 58291 58234 ID=Msa1363020-mRNA-1:cds;Parent=Msa1363020-mRNA-1
tig0018025 CDS 58685 58787 58685 ID=Msa1363020-mRNA-1:cds;Parent=Msa1363020-mRNA-1
tig0018025 three_prime_UTR 58788 58994 58788 ID=Msa1363020-mRNA-1:three_prime_utr;Parent=Msa1363020-mRNA-1
Gene Sequence

>Msa1363020

ATGTCGACTTCATCGGACATTTCTGGTTTTTCCGGCAACAAACTGATAAAGTCGTCGGAGAAACCTACGTTTTCTCAAACTTGTAATCTATTGAGCCAATACCTCAAGGAGAAGAAGGGCAGTTTCGAAGGCCTTAACCTTCACAACGCATGCACCCCTGAAACAAATGGGTCATCACCTGGTTCATCTTCTCACTCTGGAATAACCATGGATTTATTTCCCACAAACGTGACACCGAAAAACCTTACAACTATGGATTTTTTCTTTCCAAGAGTTATTAATCCTATGGTTAAAGAGCCAGAAACTGCACAATTGACAATGTTTTATAATGGACAAGTTATTGTGTTGGATGATTTTCCTGCTGAGAAAGTTGAGGAGCTGAAATCATTTGCTAGAACTCAGACTCAGCATTCAGATGTTCCTAATATGATCCCTCAACAACCTCCCTCACTAATTGATATGCCAATTGCTAGAAAAGCTTCACTTCGTCGATTCATGGAGAAGAGGAAAGATAGAGTTTCTGCCTACTCACCATATCAAAGAACCTTTCCTGACTCTGCTCCAGAAAAGCATGCTGACTCCGCTCCATGGCTTGTGTTAGGTGCTAAATCAATGTAA

Protein sequence

>Msa1363020

MSTSSDISGFSGNKLIKSSEKPTFSQTCNLLSQYLKEKKGSFEGLNLHNACTPETNGSSPGSSSHSGITMDLFPTNVTPKNLTTMDFFFPRVINPMVKEPETAQLTMFYNGQVIVLDDFPAEKVEELKSFARTQTQHSDVPNMIPQQPPSLIDMPIARKASLRRFMEKRKDRVSAYSPYQRTFPDSAPEKHADSAPWLVLGAKSM*