AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1322600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1322600 MtrunA17_Chr8g0355241 97.288 295 8 0 1 295 1 295 0.0 597
Msa1322600 MtrunA17_Chr3g0118791 67.266 278 90 1 19 295 1 278 4.92e-141 398
Msa1322600 MtrunA17_Chr7g0254371 66.534 251 84 0 45 295 28 278 8.64e-130 370
Msa1322600 MtrunA17_Chr4g0063451 40.339 295 140 7 35 295 33 325 2.80e-65 207
Msa1322600 MtrunA17_Chr5g0424491 39.764 254 130 6 55 287 624 875 5.73e-51 179
Msa1322600 MtrunA17_Chr3g0115151 38.846 260 134 8 55 291 578 835 8.36e-49 173
Msa1322600 MtrunA17_Chr3g0115131 36.502 263 143 6 46 287 608 867 4.89e-47 168
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1322600 AT5G17770.1 67.331 251 82 0 45 295 31 281 7.71e-131 373
Msa1322600 AT5G20080.1 40.190 316 157 6 10 295 15 328 2.13e-68 216
Msa1322600 AT1G37130.1 38.224 259 138 6 56 293 657 914 4.11e-48 172
Msa1322600 AT1G77760.1 35.573 253 141 6 56 287 657 908 3.70e-45 163

Find 88 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCACATTGGATTCAGATTT+TGG 0.105970 8_4:-29748277 Msa1322600:CDS
ATCATTATAATCATTCTTTA+TGG 0.135493 8_4:-29746283 Msa1322600:three_prime_UTR
CTTCCTTCATTTGTCTAAAA+TGG 0.154232 8_4:+29748072 None:intergenic
TGTCTTTCACGTTCTTAATA+AGG 0.198260 8_4:-29747096 Msa1322600:intron
GGCAATTAAACCTGGATATC+AGG 0.204182 8_4:+29746746 Msa1322590:three_prime_UTR
ATTGCACCATTTAGAATTTC+AGG 0.220278 8_4:+29749457 None:intergenic
TCCTACTCCTAATTCGATAT+TGG 0.222016 8_4:-29748768 Msa1322600:CDS
ACACCACCATTCCATTTATT+AGG 0.270818 8_4:+29746818 Msa1322590:three_prime_UTR
GTTACAAACCTGGTCAAGTT+AGG 0.279037 8_4:-29747859 Msa1322600:CDS
TTTGACAGCCTCCTAATAAA+TGG 0.283114 8_4:-29746829 Msa1322600:intron
AGGCATTACCCCAATGTTTC+AGG 0.287455 8_4:-29747810 Msa1322600:intron
GGAAGTCCCAATATCGAATT+AGG 0.287589 8_4:+29748761 None:intergenic
CCTGGTCAAGTTAGGGCATT+TGG 0.298742 8_4:-29747851 Msa1322600:CDS
CTGTCACATTGGCGTAAATA+AGG 0.315077 8_4:+29747455 Msa1322590:three_prime_UTR
GGAATGATTGCTGGAGGTTC+AGG 0.322357 8_4:-29747830 Msa1322600:CDS
TGCCAAGTCAGGCCAGTTCT+TGG 0.337644 8_4:-29746158 Msa1322600:three_prime_UTR
CCTACTCCTAATTCGATATT+GGG 0.343680 8_4:-29748767 Msa1322600:CDS
AATTTGAATCTTGCAGCATT+GGG 0.356810 8_4:+29748797 None:intergenic
TTGGAGCCTGAAATTCTAAA+TGG 0.359265 8_4:-29749463 Msa1322600:CDS
AGTTGCTATGGCTTTATTCA+TGG 0.365210 8_4:+29746592 Msa1322590:three_prime_UTR
TTCATTTGTACCTGAAACAT+TGG 0.379448 8_4:+29747800 None:intergenic
AAACAAATTTGCCCCTTTGT+TGG 0.389440 8_4:+29746339 Msa1322590:three_prime_UTR
TCATTTGTACCTGAAACATT+GGG 0.390761 8_4:+29747801 None:intergenic
TGCTTCCATTTGCCAACAAA+GGG 0.402731 8_4:-29746351 Msa1322600:three_prime_UTR
AAATCTGAATCCAATGTGAT+TGG 0.410009 8_4:+29748280 None:intergenic
AGGGCATTTGGAATGATTGC+TGG 0.412351 8_4:-29747839 Msa1322600:CDS
AAATTTGAATCTTGCAGCAT+TGG 0.413872 8_4:+29748796 None:intergenic
AGCACCTGCCCCTGATATCC+AGG 0.426304 8_4:-29746756 Msa1322600:intron
TGCAGCATTGGGGCTGATCA+TGG 0.437050 8_4:+29748808 None:intergenic
TGGATATCAGGGGCAGGTGC+TGG 0.440181 8_4:+29746758 Msa1322590:three_prime_UTR
TTATGTAGGTTACCTTTAGT+AGG 0.448970 8_4:+29747422 Msa1322590:intron
TTTGCCCCTTTGTTGGCAAA+TGG 0.450676 8_4:+29746346 Msa1322590:three_prime_UTR
GCAATTGCTCTTGTAGCTGT+TGG 0.456782 8_4:-29749439 Msa1322600:CDS
GGCTCCAAGTTCTGAGCAAA+AGG 0.457665 8_4:+29749478 None:intergenic
CCTTCATTTGTCTAAAATGG+TGG 0.459897 8_4:+29748075 None:intergenic
CAAATGTGTATTTATAAAGC+AGG 0.465550 8_4:-29749592 Msa1322600:five_prime_UTR
CCAAATGCCCTAACTTGACC+AGG 0.467529 8_4:+29747851 None:intergenic
TCGATATTGGGACTTCCTGT+TGG 0.490436 8_4:-29748755 Msa1322600:CDS
TGCCAAGAACTGGCCTGACT+TGG 0.491460 8_4:+29746156 Msa1322590:three_prime_UTR
GCAATTAAACCTGGATATCA+GGG 0.495091 8_4:+29746747 Msa1322590:three_prime_UTR
GCAACTCATCTTGCAGCACT+TGG 0.495897 8_4:-29746575 Msa1322600:CDS
AGGTGACAGCTTGGCCGTGA+AGG 0.496523 8_4:-29748051 Msa1322600:CDS
TTACAAACCTGGTCAAGTTA+GGG 0.508630 8_4:-29747858 Msa1322600:CDS
CTTCATTTGTCTAAAATGGT+GGG 0.509453 8_4:+29748076 None:intergenic
AAGCTAAGTGTCATGTTGAA+TGG 0.510044 8_4:+29749523 None:intergenic
AGTAAATATAAGCAACCGTT+AGG 0.512828 8_4:+29749416 None:intergenic
ATACACAAACTTGCCAAGTC+AGG 0.512876 8_4:-29746169 Msa1322600:three_prime_UTR
TTGCTTCCATTTGCCAACAA+AGG 0.513964 8_4:-29746352 Msa1322600:three_prime_UTR
TGCAAGCAGATGTACCCAAA+TGG 0.517984 8_4:-29748107 Msa1322600:intron
GGTGACAGCTTGGCCGTGAA+GGG 0.523033 8_4:-29748050 Msa1322600:CDS
TCTTGTAGCTGTTGGCCTAA+CGG 0.523764 8_4:-29749431 Msa1322600:CDS
CCACCATTCCATTTATTAGG+AGG 0.525573 8_4:+29746821 Msa1322590:three_prime_UTR
ATGGCTTTATTCATGGGTGG+TGG 0.526824 8_4:+29746599 Msa1322590:three_prime_UTR
AATAAATGGAATGGTGGTGT+TGG 0.539466 8_4:-29746815 Msa1322600:CDS
CTTGGCCGTGAAGGGCCCTA+AGG 0.540410 8_4:-29748042 Msa1322600:intron
GATATCGTCCACTGTCACAT+TGG 0.541108 8_4:+29747444 Msa1322590:three_prime_UTR
AAGTACCTTAGGGCCCTTCA+CGG 0.543318 8_4:+29748037 None:intergenic
GTTGCTATGGCTTTATTCAT+GGG 0.544781 8_4:+29746593 Msa1322590:three_prime_UTR
ATAAATGGAATGGTGGTGTT+GGG 0.556556 8_4:-29746814 Msa1322600:CDS
AGTGGACGATATCCTACTAA+AGG 0.561823 8_4:-29747434 Msa1322600:intron
TAATAATAACTACACCTTTG+GGG 0.566489 8_4:+29749383 None:intergenic
AATGAAGGAAGGTGACAGCT+TGG 0.567079 8_4:-29748060 Msa1322600:CDS
ATCATATACTCCAATCACAT+TGG 0.569714 8_4:-29748290 Msa1322600:CDS
TGATTATAATGATTGAAAGA+AGG 0.570757 8_4:+29746293 Msa1322590:three_prime_UTR
TGCTGCAAGATGAGTTGCTA+TGG 0.575868 8_4:+29746580 Msa1322590:three_prime_UTR
CAGCCTCCTAATAAATGGAA+TGG 0.578707 8_4:-29746824 Msa1322600:CDS
TAAACCTGGATATCAGGGGC+AGG 0.589659 8_4:+29746752 Msa1322590:three_prime_UTR
CAAGTTCTGAGCAAAAGGTA+AGG 0.593264 8_4:+29749483 None:intergenic
ATATCGAATTAGGAGTAGGA+AGG 0.599298 8_4:+29748771 None:intergenic
CCCAATATCGAATTAGGAGT+AGG 0.601609 8_4:+29748767 None:intergenic
GCATTTGGAATGATTGCTGG+AGG 0.605362 8_4:-29747836 Msa1322600:CDS
TATCGAATTAGGAGTAGGAA+GGG 0.606000 8_4:+29748772 None:intergenic
GCTATGGCTTTATTCATGGG+TGG 0.610095 8_4:+29746596 Msa1322590:three_prime_UTR
CATTTGTACCTGAAACATTG+GGG 0.612740 8_4:+29747802 None:intergenic
AAATGTGTATTTATAAAGCA+GGG 0.612999 8_4:-29749591 Msa1322600:five_prime_UTR
TGGTGTTGGGTACATATCAA+AGG 0.615193 8_4:-29746801 Msa1322600:CDS
ACAATCATTGATCTGAGTGA+AGG 0.616710 8_4:-29746398 Msa1322600:three_prime_UTR
GGACGTTTCAGTTACAAACC+TGG 0.618497 8_4:-29747869 Msa1322600:CDS
TATTAAGAACGTGAAAGACA+TGG 0.621704 8_4:+29747099 Msa1322590:three_prime_UTR
AGCAGAGGAAGAGATAGTCA+AGG 0.623400 8_4:-29748328 Msa1322600:intron
CCTCCTAATAAATGGAATGG+TGG 0.626581 8_4:-29746821 Msa1322600:CDS
ATTTGAATCTTGCAGCATTG+GGG 0.627950 8_4:+29748798 None:intergenic
TATTTACGCCAATGTGACAG+TGG 0.658716 8_4:-29747452 Msa1322600:CDS
CAATTAAACCTGGATATCAG+GGG 0.674569 8_4:+29746748 Msa1322590:three_prime_UTR
GCTTCCATTTGCCAACAAAG+GGG 0.679004 8_4:-29746350 Msa1322600:three_prime_UTR
AAACCCAAAGGACAAAACAA+AGG 0.684135 8_4:-29747482 Msa1322600:CDS
AGCCATACTAGAAAACCCAA+AGG 0.689734 8_4:-29747494 Msa1322600:CDS
TGAAAGACATGGAATCTGTG+AGG 0.721466 8_4:+29747110 Msa1322590:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAATTCTTTGAAATTTTT+GGG + chr8_4:29746896-29746915 Msa1322590:intron 10.0%
!!! TTTAAATTCTTTGAAATTTT+TGG + chr8_4:29746897-29746916 Msa1322590:intron 10.0%
!!! TTTGTTTTTAAATTATTACT+AGG + chr8_4:29749086-29749105 None:intergenic 10.0%
!!! AATTTTTATTTTGCTTAAGA+TGG + chr8_4:29747731-29747750 None:intergenic 15.0%
!!! ACATTTAATTTTAACTCAAA+TGG - chr8_4:29748015-29748034 Msa1322600:intron 15.0%
!! ATTTATTTGTATAGATTAGA+TGG + chr8_4:29748677-29748696 None:intergenic 15.0%
!!! TATTTAATAAAATGGATTAG+CGG + chr8_4:29748790-29748809 None:intergenic 15.0%
!! TTTGCACTTATTTAATAAAA+TGG + chr8_4:29748798-29748817 None:intergenic 15.0%
!! ATCATTATAATCATTCTTTA+TGG - chr8_4:29749455-29749474 Msa1322600:CDS 15.0%
!! AAATGTGTATTTATAAAGCA+GGG - chr8_4:29746147-29746166 Msa1322600:three_prime_UTR 20.0%
!!! CTAAATTTTGTACATAATGT+TGG - chr8_4:29746187-29746206 Msa1322600:three_prime_UTR 20.0%
!! ATAATAATAACTACACCTTT+GGG + chr8_4:29746359-29746378 Msa1322590:three_prime_UTR 20.0%
!! AATAATAATAACTACACCTT+TGG + chr8_4:29746360-29746379 Msa1322590:three_prime_UTR 20.0%
!!! TAATTCGGTTTTAAAAACAT+TGG + chr8_4:29746503-29746522 Msa1322590:three_prime_UTR 20.0%
!!! TAATCTCTAAAAAAATCAAG+CGG + chr8_4:29746667-29746686 Msa1322590:three_prime_UTR 20.0%
!!! GTATGAATCAAATATTTTCA+TGG + chr8_4:29746794-29746813 Msa1322590:three_prime_UTR 20.0%
!! CAAATCTAAATTTGTCAATA+AGG - chr8_4:29747085-29747104 Msa1322600:intron 20.0%
!! GTGTTTGCTTAAAAATAAAA+GGG + chr8_4:29747160-29747179 Msa1322590:intron 20.0%
!!! ATTTTTTCAACCAATAATTG+TGG + chr8_4:29747190-29747209 Msa1322590:intron 20.0%
!!! AAATTTTTGTGGACAAATTT+TGG - chr8_4:29747511-29747530 Msa1322600:intron 20.0%
!! TGAAAATGTAAGTATAACAA+TGG - chr8_4:29747829-29747848 Msa1322600:CDS 20.0%
!!! ATCAAACTTTATTTAACTCT+CGG - chr8_4:29748109-29748128 Msa1322600:intron 20.0%
!! ACTTTCAAACAATTATAACA+TGG + chr8_4:29748169-29748188 None:intergenic 20.0%
!! ACATGATTTCTTAATTATGT+AGG + chr8_4:29748333-29748352 None:intergenic 20.0%
!! TTGTTACATCTATATATGTT+TGG - chr8_4:29748567-29748586 Msa1322600:intron 20.0%
!!! TTTCTTTTTACATTCGTATT+TGG + chr8_4:29748739-29748758 None:intergenic 20.0%
!! GAAAATTGATTATTGTTAAC+TGG + chr8_4:29749016-29749035 None:intergenic 20.0%
!! TTGTATATGTCAATATGAAA+AGG + chr8_4:29749042-29749061 None:intergenic 20.0%
!!! GTTTTTAAATTATTACTAGG+TGG + chr8_4:29749083-29749102 None:intergenic 20.0%
!! TGATTATAATGATTGAAAGA+AGG + chr8_4:29749448-29749467 None:intergenic 20.0%
!!! ATTTTGCTCATTAATATTAG+AGG + chr8_4:29749549-29749568 None:intergenic 20.0%
! CAAATGTGTATTTATAAAGC+AGG - chr8_4:29746146-29746165 Msa1322600:three_prime_UTR 25.0%
! TAATAATAACTACACCTTTG+GGG + chr8_4:29746358-29746377 Msa1322590:three_prime_UTR 25.0%
! TGATGATTCAATGTGTAATT+CGG + chr8_4:29746518-29746537 Msa1322590:three_prime_UTR 25.0%
! ATGTCAATTCTATGTTCAAA+CGG - chr8_4:29746626-29746645 Msa1322600:intron 25.0%
! CTTGAAAAATTAGTCCTATA+AGG - chr8_4:29746764-29746783 Msa1322600:CDS 25.0%
!!! TGAAAATTTTGTCGTTTAGA+TGG + chr8_4:29746861-29746880 Msa1322590:intron 25.0%
!! ATCTAAACGACAAAATTTTC+AGG - chr8_4:29746860-29746879 Msa1322600:intron 25.0%
! GGTGTTTGCTTAAAAATAAA+AGG + chr8_4:29747161-29747180 Msa1322590:intron 25.0%
!!! CTATTTTGAATTAGTTGTGA+AGG - chr8_4:29747484-29747503 Msa1322600:CDS 25.0%
! TATTACCATAGGAATATAGT+TGG - chr8_4:29748396-29748415 Msa1322600:intron 25.0%
! ATTTAATTCTCTTCTACATG+AGG + chr8_4:29748472-29748491 None:intergenic 25.0%
!! GTGCAAAACTAATTTTATCT+TGG - chr8_4:29748811-29748830 Msa1322600:CDS 25.0%
!!! TATCTTGGACAAACATTTTT+TGG - chr8_4:29748826-29748845 Msa1322600:CDS 25.0%
!!! ATCTTGGACAAACATTTTTT+GGG - chr8_4:29748827-29748846 Msa1322600:CDS 25.0%
! TTTAAATTATTACTAGGTGG+TGG + chr8_4:29749080-29749099 None:intergenic 25.0%
! AAAGATTAAAAATCGCAACA+AGG - chr8_4:29749317-29749336 Msa1322600:intron 25.0%
! ATTGCACCATTTAGAATTTC+AGG + chr8_4:29746284-29746303 Msa1322590:three_prime_UTR 30.0%
AGTAAATATAAGCAACCGTT+AGG + chr8_4:29746325-29746344 Msa1322590:three_prime_UTR 30.0%
AACATATGAACCATGATACA+TGG + chr8_4:29746544-29746563 Msa1322590:three_prime_UTR 30.0%
! CAGTTTTACCTTGATTCAAA+CGG + chr8_4:29746572-29746591 Msa1322590:three_prime_UTR 30.0%
! ATTACATTCGACTTAACTTG+TGG - chr8_4:29746683-29746702 Msa1322600:intron 30.0%
!! TTACATTCGACTTAACTTGT+GGG - chr8_4:29746684-29746703 Msa1322600:intron 30.0%
! ATTTTCATGGTCAACCTTAT+AGG + chr8_4:29746781-29746800 Msa1322590:three_prime_UTR 30.0%
AATTTGAATCTTGCAGCATT+GGG + chr8_4:29746944-29746963 Msa1322590:intron 30.0%
AAATTTGAATCTTGCAGCAT+TGG + chr8_4:29746945-29746964 Msa1322590:intron 30.0%
ATACAACTAGAGCAATACAT+GGG + chr8_4:29747042-29747061 Msa1322590:intron 30.0%
AGTGTATAGCTTAGAATAGT+TGG + chr8_4:29747122-29747141 Msa1322590:three_prime_UTR 30.0%
TTTCAACCAATAATTGTGGT+GGG + chr8_4:29747186-29747205 Msa1322590:intron 30.0%
! TTTTCAACCAATAATTGTGG+TGG + chr8_4:29747187-29747206 Msa1322590:intron 30.0%
GTCATTCAAATAGACCTTAT+GGG + chr8_4:29747224-29747243 Msa1322590:intron 30.0%
TGTCATTCAAATAGACCTTA+TGG + chr8_4:29747225-29747244 Msa1322590:intron 30.0%
ATAGCAAAAGATATGTCAAG+TGG - chr8_4:29747292-29747311 Msa1322600:intron 30.0%
TTTGTATAAATGTTGAGCAG+AGG - chr8_4:29747395-29747414 Msa1322600:intron 30.0%
ATCATATACTCCAATCACAT+TGG - chr8_4:29747448-29747467 Msa1322600:CDS 30.0%
AAATCTGAATCCAATGTGAT+TGG + chr8_4:29747461-29747480 Msa1322590:three_prime_UTR 30.0%
! ATCACATTGGATTCAGATTT+TGG - chr8_4:29747461-29747480 Msa1322600:CDS 30.0%
!! GTGAAGGTACAAAATTTTTG+TGG - chr8_4:29747500-29747519 Msa1322600:CDS 30.0%
! CTTCATTTGTCTAAAATGGT+GGG + chr8_4:29747665-29747684 None:intergenic 30.0%
CTTCCTTCATTTGTCTAAAA+TGG + chr8_4:29747669-29747688 None:intergenic 30.0%
! CATTTTAGACAAATGAAGGA+AGG - chr8_4:29747667-29747686 Msa1322600:intron 30.0%
! TTTAGAGCTTCAATTTCTTG+GGG + chr8_4:29747773-29747792 None:intergenic 30.0%
! GTTTAGAGCTTCAATTTCTT+GGG + chr8_4:29747774-29747793 None:intergenic 30.0%
! TGTTTAGAGCTTCAATTTCT+TGG + chr8_4:29747775-29747794 None:intergenic 30.0%
!! TAAGTATAACAATGGATTGC+TGG - chr8_4:29747837-29747856 Msa1322600:CDS 30.0%
TCATTTGTACCTGAAACATT+GGG + chr8_4:29747940-29747959 None:intergenic 30.0%
TTCATTTGTACCTGAAACAT+TGG + chr8_4:29747941-29747960 None:intergenic 30.0%
! TACAAATGAATCTGCCATTT+AGG - chr8_4:29747951-29747970 Msa1322600:intron 30.0%
CTGCAACAAAAGATAATAAC+AGG + chr8_4:29748216-29748235 None:intergenic 30.0%
TTATGTAGGTTACCTTTAGT+AGG + chr8_4:29748319-29748338 None:intergenic 30.0%
TATAGAACTGCTATTACCAT+AGG - chr8_4:29748385-29748404 Msa1322600:intron 30.0%
TCTTACCAACTATATTCCTA+TGG + chr8_4:29748404-29748423 None:intergenic 30.0%
TATTAAGAACGTGAAAGACA+TGG + chr8_4:29748642-29748661 None:intergenic 30.0%
TGTCTTTCACGTTCTTAATA+AGG - chr8_4:29748642-29748661 Msa1322600:intron 30.0%
! CTTTTTACATTCGTATTTGG+AGG + chr8_4:29748736-29748755 None:intergenic 30.0%
! ATTTTGCAGATACTTAGATG+TGG - chr8_4:29749115-29749134 Msa1322600:intron 30.0%
AAGCTAAGTGTCATGTTGAA+TGG + chr8_4:29746218-29746237 Msa1322590:three_prime_UTR 35.0%
GTAAGGACTTGAAAAATGCA+GGG + chr8_4:29746241-29746260 Msa1322590:three_prime_UTR 35.0%
TTGGAGCCTGAAATTCTAAA+TGG - chr8_4:29746275-29746294 Msa1322600:three_prime_UTR 35.0%
! AGAGCACTTATAAGATGAGA+TGG - chr8_4:29746461-29746480 Msa1322600:three_prime_UTR 35.0%
ATCATCAAGACCATGTATCA+TGG - chr8_4:29746531-29746550 Msa1322600:exon 35.0%
! GTTTTACACCGTTTGAATCA+AGG - chr8_4:29746561-29746580 Msa1322600:CDS 35.0%
AAAAATCAAGCGGAACTAGA+TGG + chr8_4:29746657-29746676 Msa1322590:three_prime_UTR 35.0%
!!! GACAAAATTTTCAGGTAGCT+TGG - chr8_4:29746868-29746887 Msa1322600:intron 35.0%
ATTTGAATCTTGCAGCATTG+GGG + chr8_4:29746943-29746962 Msa1322590:intron 35.0%
TATCGAATTAGGAGTAGGAA+GGG + chr8_4:29746969-29746988 Msa1322590:intron 35.0%
ATATCGAATTAGGAGTAGGA+AGG + chr8_4:29746970-29746989 Msa1322590:intron 35.0%
TCCTACTCCTAATTCGATAT+TGG - chr8_4:29746970-29746989 Msa1322600:intron 35.0%
CCTACTCCTAATTCGATATT+GGG - chr8_4:29746971-29746990 Msa1322600:intron 35.0%
!!! GCAAGTATGTTTTTTCCAAC+AGG + chr8_4:29747001-29747020 Msa1322590:intron 35.0%
TTGGAAAAAACATACTTGCC+AGG - chr8_4:29747002-29747021 Msa1322600:intron 35.0%
TACAACTAGAGCAATACATG+GGG + chr8_4:29747041-29747060 Msa1322590:intron 35.0%
GATACAACTAGAGCAATACA+TGG + chr8_4:29747043-29747062 Msa1322590:intron 35.0%
AATTGCACTCAAATCCCATA+AGG - chr8_4:29747207-29747226 Msa1322600:intron 35.0%
! ACATCTCTCTACTTCTAGAA+AGG - chr8_4:29747244-29747263 Msa1322600:intron 35.0%
! TCTACTTCTAGAAAGGATCT+TGG - chr8_4:29747251-29747270 Msa1322600:intron 35.0%
! CCTTCATTTGTCTAAAATGG+TGG + chr8_4:29747666-29747685 None:intergenic 35.0%
! CCACCATTTTAGACAAATGA+AGG - chr8_4:29747663-29747682 Msa1322600:intron 35.0%
!! AGATGGTAGAAAGTACCTTA+GGG + chr8_4:29747714-29747733 None:intergenic 35.0%
!! AAGATGGTAGAAAGTACCTT+AGG + chr8_4:29747715-29747734 None:intergenic 35.0%
TTAGAGCTTCAATTTCTTGG+GGG + chr8_4:29747772-29747791 None:intergenic 35.0%
TTACAAACCTGGTCAAGTTA+GGG - chr8_4:29747880-29747899 Msa1322600:intron 35.0%
CATTTGTACCTGAAACATTG+GGG + chr8_4:29747939-29747958 None:intergenic 35.0%
AGAATCGTTCAAGAAAACCT+AGG - chr8_4:29748059-29748078 Msa1322600:CDS 35.0%
GAAAACCTAGGTTTGATTCT+TGG - chr8_4:29748071-29748090 Msa1322600:CDS 35.0%
TTCATCCAAGAATCAAACCT+AGG + chr8_4:29748079-29748098 None:intergenic 35.0%
AAACCCAAAGGACAAAACAA+AGG - chr8_4:29748256-29748275 Msa1322600:CDS 35.0%
!!! ACACCTTTGTTTTGTCCTTT+GGG + chr8_4:29748262-29748281 None:intergenic 35.0%
!!! TACACCTTTGTTTTGTCCTT+TGG + chr8_4:29748263-29748282 None:intergenic 35.0%
!! AGCAAGAAAGTACTACTCTA+AGG + chr8_4:29748356-29748375 None:intergenic 35.0%
!! CTATATATGTTTGGTGATGC+AGG - chr8_4:29748576-29748595 Msa1322600:intron 35.0%
TTTGACAGCCTCCTAATAAA+TGG - chr8_4:29748909-29748928 Msa1322600:intron 35.0%
ACACCACCATTCCATTTATT+AGG + chr8_4:29748923-29748942 None:intergenic 35.0%
! AATAAATGGAATGGTGGTGT+TGG - chr8_4:29748923-29748942 Msa1322600:intron 35.0%
! ATAAATGGAATGGTGGTGTT+GGG - chr8_4:29748924-29748943 Msa1322600:intron 35.0%
CAATTAAACCTGGATATCAG+GGG + chr8_4:29748993-29749012 None:intergenic 35.0%
GCAATTAAACCTGGATATCA+GGG + chr8_4:29748994-29749013 None:intergenic 35.0%
TGTTAACTGGCAATTAAACC+TGG + chr8_4:29749003-29749022 None:intergenic 35.0%
!! GTTGCTATGGCTTTATTCAT+GGG + chr8_4:29749148-29749167 None:intergenic 35.0%
!! AGTTGCTATGGCTTTATTCA+TGG + chr8_4:29749149-29749168 None:intergenic 35.0%
ACAATCATTGATCTGAGTGA+AGG - chr8_4:29749340-29749359 Msa1322600:intron 35.0%
AAACAAATTTGCCCCTTTGT+TGG + chr8_4:29749402-29749421 None:intergenic 35.0%
! AAATTTTCAGTGCCAAGAAC+TGG + chr8_4:29749595-29749614 None:intergenic 35.0%
GGTAAGGACTTGAAAAATGC+AGG + chr8_4:29746242-29746261 Msa1322590:three_prime_UTR 40.0%
!! CAAGTTCTGAGCAAAAGGTA+AGG + chr8_4:29746258-29746277 Msa1322590:three_prime_UTR 40.0%
! CTTACCTTTTGCTCAGAACT+TGG - chr8_4:29746256-29746275 Msa1322600:three_prime_UTR 40.0%
TACTATCGCAAAAACCCCAA+AGG - chr8_4:29746341-29746360 Msa1322600:three_prime_UTR 40.0%
CCCAATATCGAATTAGGAGT+AGG + chr8_4:29746974-29746993 Msa1322590:intron 40.0%
GGAAGTCCCAATATCGAATT+AGG + chr8_4:29746980-29746999 Msa1322590:intron 40.0%
!!! TGGGGAATCTTTTTCAAACC+TGG + chr8_4:29747023-29747042 Msa1322590:intron 40.0%
!! AACCAATAATTGTGGTGGGT+GGG + chr8_4:29747182-29747201 Msa1322590:intron 40.0%
! ACTTCTAGAAAGGATCTTGG+AGG - chr8_4:29747254-29747273 Msa1322600:intron 40.0%
! GGTGGGACATTTTTCCATTT+GGG + chr8_4:29747648-29747667 None:intergenic 40.0%
! TGGTGGGACATTTTTCCATT+TGG + chr8_4:29747649-29747668 None:intergenic 40.0%
GTTACAAACCTGGTCAAGTT+AGG - chr8_4:29747879-29747898 Msa1322600:intron 40.0%
! TGCCATTTAGGCACTTGAAA+AGG - chr8_4:29747963-29747982 Msa1322600:intron 40.0%
!!! CACCTTTTCAAGTGCCTAAA+TGG + chr8_4:29747968-29747987 None:intergenic 40.0%
AGCCATACTAGAAAACCCAA+AGG - chr8_4:29748244-29748263 Msa1322600:intron 40.0%
!! GTCCTTTGGGTTTTCTAGTA+TGG + chr8_4:29748249-29748268 None:intergenic 40.0%
CTGTCACATTGGCGTAAATA+AGG + chr8_4:29748286-29748305 None:intergenic 40.0%
TATTTACGCCAATGTGACAG+TGG - chr8_4:29748286-29748305 Msa1322600:CDS 40.0%
AGTGGACGATATCCTACTAA+AGG - chr8_4:29748304-29748323 Msa1322600:CDS 40.0%
TGAAAGACATGGAATCTGTG+AGG + chr8_4:29748631-29748650 None:intergenic 40.0%
CAGCCTCCTAATAAATGGAA+TGG - chr8_4:29748914-29748933 Msa1322600:intron 40.0%
CCACCATTCCATTTATTAGG+AGG + chr8_4:29748920-29748939 None:intergenic 40.0%
CCTCCTAATAAATGGAATGG+TGG - chr8_4:29748917-29748936 Msa1322600:intron 40.0%
! TGGTGTTGGGTACATATCAA+AGG - chr8_4:29748937-29748956 Msa1322600:intron 40.0%
GGCAATTAAACCTGGATATC+AGG + chr8_4:29748995-29749014 None:intergenic 40.0%
!! TAGGTGGTGGTGATTTTTCT+TGG + chr8_4:29749067-29749086 None:intergenic 40.0%
TTGCTTCCATTTGCCAACAA+AGG - chr8_4:29749386-29749405 Msa1322600:intron 40.0%
TGCTTCCATTTGCCAACAAA+GGG - chr8_4:29749387-29749406 Msa1322600:intron 40.0%
ATACACAAACTTGCCAAGTC+AGG - chr8_4:29749569-29749588 Msa1322600:five_prime_UTR 40.0%
GCAATTGCTCTTGTAGCTGT+TGG - chr8_4:29746299-29746318 Msa1322600:three_prime_UTR 45.0%
! TCTTGTAGCTGTTGGCCTAA+CGG - chr8_4:29746307-29746326 Msa1322600:three_prime_UTR 45.0%
TCGATATTGGGACTTCCTGT+TGG - chr8_4:29746983-29747002 Msa1322600:intron 45.0%
! CAACCAATAATTGTGGTGGG+TGG + chr8_4:29747183-29747202 Msa1322590:intron 45.0%
AGCAGAGGAAGAGATAGTCA+AGG - chr8_4:29747410-29747429 Msa1322600:intron 45.0%
TGCAAGCAGATGTACCCAAA+TGG - chr8_4:29747631-29747650 Msa1322600:intron 45.0%
AATGAAGGAAGGTGACAGCT+TGG - chr8_4:29747678-29747697 Msa1322600:intron 45.0%
!! AATGGATTGCTGGAGCTTGT+AGG - chr8_4:29747847-29747866 Msa1322600:CDS 45.0%
! ATGGATTGCTGGAGCTTGTA+GGG - chr8_4:29747848-29747867 Msa1322600:CDS 45.0%
GGACGTTTCAGTTACAAACC+TGG - chr8_4:29747869-29747888 Msa1322600:CDS 45.0%
! AGGGCATTTGGAATGATTGC+TGG - chr8_4:29747899-29747918 Msa1322600:intron 45.0%
! GCATTTGGAATGATTGCTGG+AGG - chr8_4:29747902-29747921 Msa1322600:intron 45.0%
AGGCATTACCCCAATGTTTC+AGG - chr8_4:29747928-29747947 Msa1322600:intron 45.0%
GATATCGTCCACTGTCACAT+TGG + chr8_4:29748297-29748316 None:intergenic 45.0%
!! ATGGCTTTATTCATGGGTGG+TGG + chr8_4:29749142-29749161 None:intergenic 45.0%
!! GCTATGGCTTTATTCATGGG+TGG + chr8_4:29749145-29749164 None:intergenic 45.0%
TGCTGCAAGATGAGTTGCTA+TGG + chr8_4:29749161-29749180 None:intergenic 45.0%
GCTTCCATTTGCCAACAAAG+GGG - chr8_4:29749388-29749407 Msa1322600:intron 45.0%
TTTGCCCCTTTGTTGGCAAA+TGG + chr8_4:29749395-29749414 None:intergenic 45.0%
!!! TTTTTTTCAAATATTTATTA+AGG + chr8_4:29747347-29747366 Msa1322590:intron 5.0%
!! AATAAATATTTGAAAAAAAA+AGG - chr8_4:29747348-29747367 Msa1322600:intron 5.0%
!! AAATAATTATAATATGAATT+CGG + chr8_4:29748135-29748154 None:intergenic 5.0%
!! TAATAATTTAAAAACAAAAA+TGG - chr8_4:29749088-29749107 Msa1322600:intron 5.0%
!! GGCTCCAAGTTCTGAGCAAA+AGG + chr8_4:29746263-29746282 Msa1322590:three_prime_UTR 50.0%
CACCCACCCACCACAATTAT+TGG - chr8_4:29747177-29747196 Msa1322600:intron 50.0%
AAGTACCTTAGGGCCCTTCA+CGG + chr8_4:29747704-29747723 None:intergenic 50.0%
CCAAATGCCCTAACTTGACC+AGG + chr8_4:29747890-29747909 None:intergenic 50.0%
! CCTGGTCAAGTTAGGGCATT+TGG - chr8_4:29747887-29747906 Msa1322600:intron 50.0%
GGAATGATTGCTGGAGGTTC+AGG - chr8_4:29747908-29747927 Msa1322600:intron 50.0%
TAAACCTGGATATCAGGGGC+AGG + chr8_4:29748989-29749008 None:intergenic 50.0%
GCAACTCATCTTGCAGCACT+TGG - chr8_4:29749163-29749182 Msa1322600:intron 50.0%
! TGCAGCATTGGGGCTGATCA+TGG + chr8_4:29746933-29746952 Msa1322590:intron 55.0%
TGCCAAGTCAGGCCAGTTCT+TGG - chr8_4:29749580-29749599 Msa1322600:five_prime_UTR 55.0%
! TGCCAAGAACTGGCCTGACT+TGG + chr8_4:29749585-29749604 None:intergenic 55.0%
AGGTGACAGCTTGGCCGTGA+AGG - chr8_4:29747687-29747706 Msa1322600:intron 60.0%
GGTGACAGCTTGGCCGTGAA+GGG - chr8_4:29747688-29747707 Msa1322600:intron 60.0%
TGGATATCAGGGGCAGGTGC+TGG + chr8_4:29748983-29749002 None:intergenic 60.0%
AGCACCTGCCCCTGATATCC+AGG - chr8_4:29748982-29749001 Msa1322600:intron 60.0%
CTTGGCCGTGAAGGGCCCTA+AGG - chr8_4:29747696-29747715 Msa1322600:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 29746145 29749615 29746145 ID=Msa1322600;Name=Msa1322600
chr8_4 mRNA 29746145 29749615 29746145 ID=Msa1322600-mRNA-1;Parent=Msa1322600;Name=Msa1322600-mRNA-1;_AED=0.08;_eAED=0.08;_QI=58|1|1|1|1|1|9|399|295
chr8_4 exon 29746145 29746636 29746145 ID=Msa1322600-mRNA-1:exon:9934;Parent=Msa1322600-mRNA-1
chr8_4 exon 29746757 29746843 29746757 ID=Msa1322600-mRNA-1:exon:9933;Parent=Msa1322600-mRNA-1
chr8_4 exon 29747097 29747162 29747097 ID=Msa1322600-mRNA-1:exon:9932;Parent=Msa1322600-mRNA-1
chr8_4 exon 29747435 29747524 29747435 ID=Msa1322600-mRNA-1:exon:9931;Parent=Msa1322600-mRNA-1
chr8_4 exon 29747811 29747891 29747811 ID=Msa1322600-mRNA-1:exon:9930;Parent=Msa1322600-mRNA-1
chr8_4 exon 29748043 29748120 29748043 ID=Msa1322600-mRNA-1:exon:9929;Parent=Msa1322600-mRNA-1
chr8_4 exon 29748255 29748345 29748255 ID=Msa1322600-mRNA-1:exon:9928;Parent=Msa1322600-mRNA-1
chr8_4 exon 29748737 29748878 29748737 ID=Msa1322600-mRNA-1:exon:9927;Parent=Msa1322600-mRNA-1
chr8_4 exon 29749398 29749615 29749398 ID=Msa1322600-mRNA-1:exon:9926;Parent=Msa1322600-mRNA-1
chr8_4 five_prime_UTR 29749558 29749615 29749558 ID=Msa1322600-mRNA-1:five_prime_utr;Parent=Msa1322600-mRNA-1
chr8_4 CDS 29749398 29749557 29749398 ID=Msa1322600-mRNA-1:cds;Parent=Msa1322600-mRNA-1
chr8_4 CDS 29748737 29748878 29748737 ID=Msa1322600-mRNA-1:cds;Parent=Msa1322600-mRNA-1
chr8_4 CDS 29748255 29748345 29748255 ID=Msa1322600-mRNA-1:cds;Parent=Msa1322600-mRNA-1
chr8_4 CDS 29748043 29748120 29748043 ID=Msa1322600-mRNA-1:cds;Parent=Msa1322600-mRNA-1
chr8_4 CDS 29747811 29747891 29747811 ID=Msa1322600-mRNA-1:cds;Parent=Msa1322600-mRNA-1
chr8_4 CDS 29747435 29747524 29747435 ID=Msa1322600-mRNA-1:cds;Parent=Msa1322600-mRNA-1
chr8_4 CDS 29747097 29747162 29747097 ID=Msa1322600-mRNA-1:cds;Parent=Msa1322600-mRNA-1
chr8_4 CDS 29746757 29746843 29746757 ID=Msa1322600-mRNA-1:cds;Parent=Msa1322600-mRNA-1
chr8_4 CDS 29746544 29746636 29746544 ID=Msa1322600-mRNA-1:cds;Parent=Msa1322600-mRNA-1
chr8_4 three_prime_UTR 29746145 29746543 29746145 ID=Msa1322600-mRNA-1:three_prime_utr;Parent=Msa1322600-mRNA-1
Gene Sequence

>Msa1322600

ATGTTGGAGTTACCATTCAACATGACACTTAGCTTCCCTGCATTTTTCAAGTCCTTACCTTTTGCTCAGAACTTGGAGCCTGAAATTCTAAATGGTGCAATTGCTCTTGTAGCTGTTGGCCTAACGGTTGCTTATATTTACTATCGCAAAAACCCCAAAGGTAGCTTGGATCCCAAAAATTTCAAAGAATTTAAACTTGTAAAGAAGACCATGATCAGCCCCAATGCTGCAAGATTCAAATTTGCCCTTCCTACTCCTAATTCGATATTGGGACTTCCTGTTGGAAAAAACATACTTGCCAGAGGAAGAGATAGTCAAGGAGAAGAAGTTATGAGATCATATACTCCAATCACATTGGATTCAGATTTTGGCTATTTTGAATTAGTTGTGAAGATGTACCCAAATGGAAAAATGTCCCACCATTTTAGACAAATGAAGGAAGGTGACAGCTTGGCCGTGAAGGGCCCTAAGGGACGTTTCAGTTACAAACCTGGTCAAGTTAGGGCATTTGGAATGATTGCTGGAGGTTCAGGCATTACCCCAATGTTTCAGATCATAAGAGCCATACTAGAAAACCCAAAGGACAAAACAAAGGTGTACCTTATTTACGCCAATGTGACAGTGGACGATATCCTACTAAAGGAAGAACTAGATCGCTTTGCAGACAAATTTCCTCACAGATTCCATGTCTTTCACGTTCTTAATAAGCCTCCTAATAAATGGAATGGTGGTGTTGGGTACATATCAAAGGAGATGATTGAATCTCATTGTCCAGCACCTGCCCCTGATATCCAGATACTTAGATGTGGTCCACCACCCATGAATAAAGCCATAGCAACTCATCTTGCAGCACTTGGATACACATCAAACATGCAGTTCGAGTTCTAA
Protein sequence

>Msa1322600

MLELPFNMTLSFPAFFKSLPFAQNLEPEILNGAIALVAVGLTVAYIYYRKNPKGSLDPKNFKEFKLVKKTMISPNAARFKFALPTPNSILGLPVGKNILARGRDSQGEEVMRSYTPITLDSDFGYFELVVKMYPNGKMSHHFRQMKEGDSLAVKGPKGRFSYKPGQVRAFGMIAGGSGITPMFQIIRAILENPKDKTKVYLIYANVTVDDILLKEELDRFADKFPHRFHVFHVLNKPPNKWNGGVGYISKEMIESHCPAPAPDIQILRCGPPPMNKAIATHLAALGYTSNMQFEF*