AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1283730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1283730 MtrunA17_Chr8g0355241 98.305 295 5 0 1 295 1 295 0.0 601
Msa1283730 MtrunA17_Chr3g0118791 67.266 278 90 1 19 295 1 278 1.88e-141 399
Msa1283730 MtrunA17_Chr7g0254371 66.534 251 84 0 45 295 28 278 2.83e-130 371
Msa1283730 MtrunA17_Chr4g0063451 40.339 295 140 7 35 295 33 325 2.68e-65 207
Msa1283730 MtrunA17_Chr5g0424491 39.526 253 132 5 55 287 624 875 1.20e-51 181
Msa1283730 MtrunA17_Chr3g0115151 38.846 260 134 8 55 291 578 835 5.69e-49 174
Msa1283730 MtrunA17_Chr3g0115131 36.680 259 140 6 50 287 612 867 3.47e-47 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1283730 AT5G17770.1 67.331 251 82 0 45 295 31 281 1.74e-131 375
Msa1283730 AT5G20080.1 40.190 316 157 6 10 295 15 328 2.76e-68 216
Msa1283730 AT1G37130.1 38.224 259 138 6 56 293 657 914 1.07e-48 174
Msa1283730 AT1G77760.1 35.968 253 140 6 56 287 657 908 4.50e-46 166

Find 89 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTTCATTTGTCTAAAA+TGG 0.154232 8_3:+25336559 None:intergenic
CCAACATTATGTACAAAATT+TGG 0.167602 8_3:+25338056 None:intergenic
TGTCTTTCACGTTCTTAATA+AGG 0.198260 8_3:-25335580 Msa1283730:intron
GGCAATTAAACCTGGATATC+AGG 0.207511 8_3:+25334680 Msa1283720:three_prime_UTR
ATTGCACCATTTAGAATTTC+AGG 0.220278 8_3:+25337962 None:intergenic
TCCTACTCCTAATTCGATAT+TGG 0.222016 8_3:-25337275 Msa1283730:CDS
ATCACATTGGATTCAGATAT+TGG 0.246714 8_3:-25336762 Msa1283730:CDS
ACACCACCATTCCATTTATT+AGG 0.270818 8_3:+25334752 Msa1283720:three_prime_UTR
GTTACAAACCTGGTCAAGTT+AGG 0.279037 8_3:-25336346 Msa1283730:CDS
TTTGACAGCCTCCTAATAAA+TGG 0.283114 8_3:-25334763 Msa1283730:intron
AGGCATTACCCCAATGTTTC+AGG 0.287455 8_3:-25336297 Msa1283730:intron
GGAAGTCCCAATATCGAATT+AGG 0.287589 8_3:+25337268 None:intergenic
CCTGGTCAAGTTAGGGCATT+TGG 0.298742 8_3:-25336338 Msa1283730:CDS
CTGTCACATTGGCGTAAATA+AGG 0.315077 8_3:+25335939 Msa1283720:three_prime_UTR
GGAATGATTGCTGGAGGTTC+AGG 0.322357 8_3:-25336317 Msa1283730:CDS
TGCCAAGTCAGGCCAGTTCT+TGG 0.337644 8_3:-25334092 Msa1283730:three_prime_UTR
CCTACTCCTAATTCGATATT+GGG 0.343680 8_3:-25337274 Msa1283730:CDS
AATTTGAATCTTGCAGCATT+GGG 0.356810 8_3:+25337304 None:intergenic
TTGGAGCCTGAAATTCTAAA+TGG 0.359265 8_3:-25337968 Msa1283730:CDS
AGTTGCTATGGCTTTATTCA+TGG 0.365210 8_3:+25334526 Msa1283720:three_prime_UTR
ATCACTATAATCATTCTATA+TGG 0.372899 8_3:-25334217 Msa1283730:three_prime_UTR
TTCATTTGTACCTGAAACAT+TGG 0.379448 8_3:+25336287 None:intergenic
TCATTTGTACCTGAAACATT+GGG 0.390761 8_3:+25336288 None:intergenic
TGCTTCCATTTGCCAACAAA+GGG 0.402731 8_3:-25334285 Msa1283730:three_prime_UTR
ATACGAATTTGCCCCTTTGT+TGG 0.407961 8_3:+25334273 Msa1283720:three_prime_UTR
AGGGCATTTGGAATGATTGC+TGG 0.412351 8_3:-25336326 Msa1283730:CDS
AAATTTGAATCTTGCAGCAT+TGG 0.413872 8_3:+25337303 None:intergenic
AGCACCTGCCCCTGATATCC+AGG 0.426304 8_3:-25334690 Msa1283730:intron
TGCAGCATTGGGGCTGATCA+TGG 0.437050 8_3:+25337315 None:intergenic
TGGATATCAGGGGCAGGTGC+TGG 0.440181 8_3:+25334692 Msa1283720:three_prime_UTR
ATATCTGAATCCAATGTGAT+TGG 0.441409 8_3:+25336765 None:intergenic
TTATGTAGGTTACCTTTAGT+AGG 0.448970 8_3:+25335906 Msa1283720:intron
GCAATTGCTCTTGTAGCTGT+TGG 0.456782 8_3:-25337944 Msa1283730:CDS
TTTGCCCCTTTGTTGGCAAA+TGG 0.457496 8_3:+25334280 Msa1283720:three_prime_UTR
GGCTCCAAGTTCTGAGCAAA+AGG 0.457665 8_3:+25337983 None:intergenic
CCTTCATTTGTCTAAAATGG+TGG 0.459897 8_3:+25336562 None:intergenic
CAAATGTGTATTTATAAAGC+AGG 0.465550 8_3:-25338097 Msa1283730:five_prime_UTR
CCAAATGCCCTAACTTGACC+AGG 0.467529 8_3:+25336338 None:intergenic
TCGATATTGGGACTTCCTGT+TGG 0.490436 8_3:-25337262 Msa1283730:CDS
TGCCAAGAACTGGCCTGACT+TGG 0.491460 8_3:+25334090 Msa1283720:three_prime_UTR
GCAACTCATCTTGCAGCACT+TGG 0.495897 8_3:-25334509 Msa1283730:CDS
GCAATTAAACCTGGATATCA+GGG 0.505823 8_3:+25334681 Msa1283720:three_prime_UTR
TTACAAACCTGGTCAAGTTA+GGG 0.508630 8_3:-25336345 Msa1283730:CDS
CTTCATTTGTCTAAAATGGT+GGG 0.509453 8_3:+25336563 None:intergenic
AAGCTAAGTGTCATGTTGAA+TGG 0.510044 8_3:+25338028 None:intergenic
AGTAAATATAAGCAACCGTT+AGG 0.512828 8_3:+25337921 None:intergenic
ATACACAAACTTGCCAAGTC+AGG 0.512876 8_3:-25334103 Msa1283730:three_prime_UTR
TTGCTTCCATTTGCCAACAA+AGG 0.513964 8_3:-25334286 Msa1283730:three_prime_UTR
TGCAAGCAGATGTACCCAAA+TGG 0.517984 8_3:-25336594 Msa1283730:intron
AGGTGACAACTTGGCCGTGA+AGG 0.522801 8_3:-25336538 Msa1283730:CDS
TCTTGTAGCTGTTGGCCTAA+CGG 0.523764 8_3:-25337936 Msa1283730:CDS
CCACCATTCCATTTATTAGG+AGG 0.525573 8_3:+25334755 Msa1283720:three_prime_UTR
GGTGACAACTTGGCCGTGAA+GGG 0.526514 8_3:-25336537 Msa1283730:CDS
ATGGCTTTATTCATGGGTGG+TGG 0.526824 8_3:+25334533 Msa1283720:three_prime_UTR
AATAAATGGAATGGTGGTGT+TGG 0.539466 8_3:-25334749 Msa1283730:CDS
CTTGGCCGTGAAGGGCCCTA+AGG 0.541711 8_3:-25336529 Msa1283730:intron
AAGTACCTTAGGGCCCTTCA+CGG 0.543318 8_3:+25336524 None:intergenic
GTTGCTATGGCTTTATTCAT+GGG 0.544781 8_3:+25334527 Msa1283720:three_prime_UTR
AGTGGATGATATCCTACTAA+AGG 0.546965 8_3:-25335918 Msa1283730:intron
AATGAAGGAAGGTGACAACT+TGG 0.552525 8_3:-25336547 Msa1283730:CDS
ATAAATGGAATGGTGGTGTT+GGG 0.556556 8_3:-25334748 Msa1283730:CDS
TAATAATAACTACACCTTTG+GGG 0.566489 8_3:+25337888 None:intergenic
ATCATATACTCCAATCACAT+TGG 0.569714 8_3:-25336775 Msa1283730:CDS
TGCTGCAAGATGAGTTGCTA+TGG 0.575868 8_3:+25334514 Msa1283720:three_prime_UTR
CAGCCTCCTAATAAATGGAA+TGG 0.578707 8_3:-25334758 Msa1283730:CDS
TGATTATAGTGATTGAAAGA+AGG 0.587279 8_3:+25334227 Msa1283720:three_prime_UTR
TAAACCTGGATATCAGGGGC+AGG 0.589659 8_3:+25334686 Msa1283720:three_prime_UTR
CAAGTTCTGAGCAAAAGGTA+AGG 0.593264 8_3:+25337988 None:intergenic
GATATCATCCACTGTCACAT+TGG 0.595031 8_3:+25335928 Msa1283720:three_prime_UTR
ATATCGAATTAGGAGTAGGA+AGG 0.599298 8_3:+25337278 None:intergenic
CCCAATATCGAATTAGGAGT+AGG 0.601609 8_3:+25337274 None:intergenic
GCATTTGGAATGATTGCTGG+AGG 0.605362 8_3:-25336323 Msa1283730:CDS
TATCGAATTAGGAGTAGGAA+GGG 0.606000 8_3:+25337279 None:intergenic
GCTATGGCTTTATTCATGGG+TGG 0.610095 8_3:+25334530 Msa1283720:three_prime_UTR
CATTTGTACCTGAAACATTG+GGG 0.612740 8_3:+25336289 None:intergenic
AAATGTGTATTTATAAAGCA+GGG 0.612999 8_3:-25338096 Msa1283730:five_prime_UTR
AGCAGAGGAAGAGATAGTCA+AGG 0.615065 8_3:-25336813 Msa1283730:intron
TGGTGTTGGGTACATATCAA+AGG 0.615193 8_3:-25334735 Msa1283730:CDS
ACAATCATTGATCTGAGTGA+AGG 0.616710 8_3:-25334332 Msa1283730:three_prime_UTR
GGACGTTTCAGTTACAAACC+TGG 0.618497 8_3:-25336356 Msa1283730:CDS
TATTAAGAACGTGAAAGACA+TGG 0.621704 8_3:+25335583 Msa1283720:three_prime_UTR
CCTCCTAATAAATGGAATGG+TGG 0.626581 8_3:-25334755 Msa1283730:CDS
ATTTGAATCTTGCAGCATTG+GGG 0.627950 8_3:+25337305 None:intergenic
TATTTACGCCAATGTGACAG+TGG 0.658716 8_3:-25335936 Msa1283730:CDS
GCTTCCATTTGCCAACAAAG+GGG 0.679004 8_3:-25334284 Msa1283730:three_prime_UTR
AAACCCAAAGGACAAAACAA+AGG 0.684135 8_3:-25335966 Msa1283730:CDS
CAATTAAACCTGGATATCAG+GGG 0.687288 8_3:+25334682 Msa1283720:three_prime_UTR
AGCCATACTAGAAAACCCAA+AGG 0.689734 8_3:-25335978 Msa1283730:CDS
TGAAAGACATGGAATCTGTG+AGG 0.721466 8_3:+25335594 Msa1283720:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAATTCTTTGAAATTTTT+GGG + chr8_3:25334828-25334847 Msa1283720:intron 10.0%
!!! TTTAAATTCTTTGAAATTTT+TGG + chr8_3:25334829-25334848 Msa1283720:intron 10.0%
!!! AATACTCTATTATTTAAAAT+TGG - chr8_3:25336649-25336668 Msa1283730:intron 10.0%
!!! ATACTCTATTATTTAAAATT+GGG - chr8_3:25336650-25336669 Msa1283730:intron 10.0%
!! TATTTAATAAAATAGATTAG+CGG + chr8_3:25337295-25337314 None:intergenic 10.0%
!!! TTTGTTTTTAAATTATTACT+AGG + chr8_3:25337591-25337610 None:intergenic 10.0%
!!! TAATTCGATTTTAAAAACAT+TGG + chr8_3:25334436-25334455 Msa1283720:three_prime_UTR 15.0%
!!! ACATTTAATTTTAACTCAAA+TGG - chr8_3:25335967-25335986 Msa1283730:CDS 15.0%
!! ATTTATTTGTATAGATTAGA+TGG + chr8_3:25336632-25336651 None:intergenic 15.0%
!! AATGCTATTAAAATTAAGTA+GGG + chr8_3:25337138-25337157 None:intergenic 15.0%
!! AAATGCTATTAAAATTAAGT+AGG + chr8_3:25337137-25337156 None:intergenic 15.0%
!!! AATTTTGCAATAATTTTTTG+CGG + chr8_3:25336881-25336900 None:intergenic 15.0%
!! AGATAAAAAGTAATAAAATC+TGG + chr8_3:25336916-25336935 None:intergenic 15.0%
!! TTCAAAAATTTAAGAGTTTA+AGG - chr8_3:25337047-25337066 Msa1283730:intron 15.0%
!! AAATGCTATTAAAATTAAGT+AGG - chr8_3:25337137-25337156 Msa1283730:intron 15.0%
!! AATGCTATTAAAATTAAGTA+GGG - chr8_3:25337138-25337157 Msa1283730:intron 15.0%
!!! ATTAAGAGCAAAATTTTAAA+GGG + chr8_3:25337212-25337231 None:intergenic 15.0%
!!! AATTAAGAGCAAAATTTTAA+AGG + chr8_3:25337213-25337232 None:intergenic 15.0%
!! GAAAATTGATTATTGTTAAT+TGG + chr8_3:25337521-25337540 None:intergenic 15.0%
!! AAATGTGTATTTATAAAGCA+GGG - chr8_3:25334081-25334100 Msa1283730:three_prime_UTR 20.0%
!! ATAATAATAACTACACCTTT+GGG + chr8_3:25334293-25334312 Msa1283720:three_prime_UTR 20.0%
!! AATAATAATAACTACACCTT+TGG + chr8_3:25334294-25334313 Msa1283720:three_prime_UTR 20.0%
!!! GTAATCTTTAAAAAATCAAG+CGG + chr8_3:25334600-25334619 Msa1283720:three_prime_UTR 20.0%
!!! GTATGAATCAAATATTTTCA+TGG + chr8_3:25334726-25334745 Msa1283720:three_prime_UTR 20.0%
!! CAAATCTAAATTTGTCAATA+AGG - chr8_3:25335017-25335036 Msa1283730:intron 20.0%
!! GTGTTTGCTTAAAAATAAAA+GGG + chr8_3:25335090-25335109 Msa1283720:intron 20.0%
!!! ATTTTTTCAACCAATAATTG+TGG + chr8_3:25335120-25335139 Msa1283720:intron 20.0%
!!! AAAATTTTGTGGACAAATTT+TGG - chr8_3:25335465-25335484 Msa1283730:intron 20.0%
!!! AATTTTTATTTTGCCTAAGA+TGG + chr8_3:25335683-25335702 Msa1283720:intron 20.0%
!! TGAAAATGTAAGTATAACAA+TGG - chr8_3:25335781-25335800 Msa1283730:intron 20.0%
!!! ATCAAACTTTATTTAACTCT+CGG - chr8_3:25336061-25336080 Msa1283730:intron 20.0%
!! ACTTTCAAACAATTATAACA+TGG + chr8_3:25336121-25336140 Msa1283720:three_prime_UTR 20.0%
!! ACATGATTTCTTAATTATGT+AGG + chr8_3:25336288-25336307 None:intergenic 20.0%
!! TTGTTACATCTATATATGTT+TGG - chr8_3:25336522-25336541 Msa1283730:intron 20.0%
!! AAACAAATTTGAATTGAGTA+GGG + chr8_3:25336786-25336805 None:intergenic 20.0%
!! TAAACAAATTTGAATTGAGT+AGG + chr8_3:25336787-25336806 None:intergenic 20.0%
!!! TTTCTTTTTACATTCGTATT+TGG + chr8_3:25337244-25337263 None:intergenic 20.0%
!! TTGTATATGTCAATATGAAA+AGG + chr8_3:25337547-25337566 None:intergenic 20.0%
!!! GTTTTTAAATTATTACTAGG+TGG + chr8_3:25337588-25337607 None:intergenic 20.0%
!! ATCACTATAATCATTCTATA+TGG - chr8_3:25337960-25337979 Msa1283730:CDS 20.0%
!!! ATTTTGCTCATTAATATTAG+AGG + chr8_3:25338054-25338073 None:intergenic 20.0%
! CAAATGTGTATTTATAAAGC+AGG - chr8_3:25334080-25334099 Msa1283730:three_prime_UTR 25.0%
! CCAACATTATGTACAAAATT+TGG + chr8_3:25334124-25334143 Msa1283720:three_prime_UTR 25.0%
!! CCAAATTTTGTACATAATGT+TGG - chr8_3:25334121-25334140 Msa1283730:three_prime_UTR 25.0%
! TAATAATAACTACACCTTTG+GGG + chr8_3:25334292-25334311 Msa1283720:three_prime_UTR 25.0%
! ATGTCAATTCTATGTTCAAA+CGG - chr8_3:25334559-25334578 Msa1283730:intron 25.0%
! CTTGAAAAATTAGTCCTTAA+AGG - chr8_3:25334696-25334715 Msa1283730:CDS 25.0%
!!! TGAAAATTTTGTCGTTTAGA+TGG + chr8_3:25334793-25334812 Msa1283720:intron 25.0%
!! ATCTAAACGACAAAATTTTC+AGG - chr8_3:25334792-25334811 Msa1283730:intron 25.0%
! TAAGTATAGCTTAGAATAGT+TGG + chr8_3:25335054-25335073 Msa1283720:intron 25.0%
! GGTGTTTGCTTAAAAATAAA+AGG + chr8_3:25335091-25335110 Msa1283720:intron 25.0%
! GATATAATGTATTTGTCAAG+TGG - chr8_3:25335247-25335266 Msa1283730:intron 25.0%
!! TTTTGTATAAATTTGAGCAG+AGG - chr8_3:25335349-25335368 Msa1283730:intron 25.0%
!!! CTATTTTGAATTAGTTGTGA+AGG - chr8_3:25335438-25335457 Msa1283730:intron 25.0%
! TATTACCATAGGAATATAGT+TGG - chr8_3:25336351-25336370 Msa1283730:CDS 25.0%
! ATTTAATTCTCTTCTACATG+AGG + chr8_3:25336427-25336446 None:intergenic 25.0%
! AGTGCAGAAAAACAAAATTA+GGG + chr8_3:25336753-25336772 None:intergenic 25.0%
!!! TAATCAAACTTTGTTTTAGC+AGG - chr8_3:25337075-25337094 Msa1283730:intron 25.0%
!!! AATCAAACTTTGTTTTAGCA+GGG - chr8_3:25337076-25337095 Msa1283730:intron 25.0%
!!! TTACAAAGTCGCTATTTTAT+AGG - chr8_3:25337168-25337187 Msa1283730:intron 25.0%
!!! TACAAAGTCGCTATTTTATA+GGG - chr8_3:25337169-25337188 Msa1283730:intron 25.0%
!! GTGCAAAACTAATTTTATCT+TGG - chr8_3:25337316-25337335 Msa1283730:CDS 25.0%
! TTTAAATTATTACTAGGTGG+TGG + chr8_3:25337585-25337604 None:intergenic 25.0%
! AAAGATTAAAAATCGCAACA+AGG - chr8_3:25337822-25337841 Msa1283730:intron 25.0%
! TGATTATAGTGATTGAAAGA+AGG + chr8_3:25337953-25337972 None:intergenic 25.0%
! ATTGCACCATTTAGAATTTC+AGG + chr8_3:25334218-25334237 Msa1283720:three_prime_UTR 30.0%
AGTAAATATAAGCAACCGTT+AGG + chr8_3:25334259-25334278 Msa1283720:three_prime_UTR 30.0%
! AAAGCACTTAAAAGATGAGA+TGG - chr8_3:25334394-25334413 Msa1283730:three_prime_UTR 30.0%
! CAGTTTTACCTTGATTCAAA+CGG + chr8_3:25334505-25334524 Msa1283720:three_prime_UTR 30.0%
! ATTACATTCGACTTAACTTG+TGG - chr8_3:25334615-25334634 Msa1283730:intron 30.0%
!! TTACATTCGACTTAACTTGT+GGG - chr8_3:25334616-25334635 Msa1283730:intron 30.0%
! ATTTTCATGGTCAACCTTTA+AGG + chr8_3:25334713-25334732 Msa1283720:three_prime_UTR 30.0%
AATTTGAATCTTGCAGCATT+GGG + chr8_3:25334876-25334895 Msa1283720:intron 30.0%
AAATTTGAATCTTGCAGCAT+TGG + chr8_3:25334877-25334896 Msa1283720:intron 30.0%
!!! ACAAGTATGTTTTTTCCAAC+AGG + chr8_3:25334933-25334952 Msa1283720:intron 30.0%
TTGGAAAAAACATACTTGTC+AGG - chr8_3:25334934-25334953 Msa1283730:intron 30.0%
ATACAACTAGAGCAATACAT+GGG + chr8_3:25334974-25334993 Msa1283720:intron 30.0%
TTTCAACCAATAATTGTGGT+GGG + chr8_3:25335116-25335135 Msa1283720:intron 30.0%
! TTTTCAACCAATAATTGTGG+TGG + chr8_3:25335117-25335136 Msa1283720:intron 30.0%
GTCATTCAAATAGACCTTAT+GGG + chr8_3:25335154-25335173 Msa1283720:intron 30.0%
TGTCATTCAAATAGACCTTA+TGG + chr8_3:25335155-25335174 Msa1283720:intron 30.0%
ATCATATACTCCAATCACAT+TGG - chr8_3:25335402-25335421 Msa1283730:intron 30.0%
ATATCTGAATCCAATGTGAT+TGG + chr8_3:25335415-25335434 Msa1283720:intron 30.0%
ATCACATTGGATTCAGATAT+TGG - chr8_3:25335415-25335434 Msa1283730:intron 30.0%
!! GTGAAGGTACAAAAATTTTG+TGG - chr8_3:25335454-25335473 Msa1283730:intron 30.0%
! CTTCATTTGTCTAAAATGGT+GGG + chr8_3:25335617-25335636 Msa1283720:three_prime_UTR 30.0%
CTTCCTTCATTTGTCTAAAA+TGG + chr8_3:25335621-25335640 Msa1283720:three_prime_UTR 30.0%
! CATTTTAGACAAATGAAGGA+AGG - chr8_3:25335619-25335638 Msa1283730:CDS 30.0%
! GTTTAGAGCTTCAATTTCTT+GGG + chr8_3:25335726-25335745 Msa1283720:intron 30.0%
! TGTTTAGAGCTTCAATTTCT+TGG + chr8_3:25335727-25335746 Msa1283720:intron 30.0%
!! TAAGTATAACAATGGATTGC+TGG - chr8_3:25335789-25335808 Msa1283730:intron 30.0%
TCATTTGTACCTGAAACATT+GGG + chr8_3:25335892-25335911 Msa1283720:intron 30.0%
TTCATTTGTACCTGAAACAT+TGG + chr8_3:25335893-25335912 Msa1283720:intron 30.0%
! TACAAATGAATCTGCCATTT+AGG - chr8_3:25335903-25335922 Msa1283730:intron 30.0%
TTTATCCAAGAATCAAACCT+AGG + chr8_3:25336031-25336050 Msa1283720:three_prime_UTR 30.0%
CTGCAACAAAAGATAATAAC+AGG + chr8_3:25336171-25336190 None:intergenic 30.0%
TTATGTAGGTTACCTTTAGT+AGG + chr8_3:25336274-25336293 None:intergenic 30.0%
TATAGAACTGCTATTACCAT+AGG - chr8_3:25336340-25336359 Msa1283730:CDS 30.0%
TCTTACCAACTATATTCCTA+TGG + chr8_3:25336359-25336378 None:intergenic 30.0%
TATTAAGAACGTGAAAGACA+TGG + chr8_3:25336597-25336616 None:intergenic 30.0%
TGTCTTTCACGTTCTTAATA+AGG - chr8_3:25336597-25336616 Msa1283730:intron 30.0%
!! TACAAGGTCGCTATTTTATA+GGG + chr8_3:25336693-25336712 None:intergenic 30.0%
!! TTACAAGGTCGCTATTTTAT+AGG + chr8_3:25336694-25336713 None:intergenic 30.0%
CAGTGCAGAAAAACAAAATT+AGG + chr8_3:25336754-25336773 None:intergenic 30.0%
CAAGATTAGAACACTATGAA+AGG + chr8_3:25337013-25337032 None:intergenic 30.0%
! CTTTTTACATTCGTATTTGG+AGG + chr8_3:25337241-25337260 None:intergenic 30.0%
TGTTAATTGGCAATTAAACC+TGG + chr8_3:25337508-25337527 None:intergenic 30.0%
! ATTTTGCAGATACTTAGATG+TGG - chr8_3:25337620-25337639 Msa1283730:intron 30.0%
AAGCTAAGTGTCATGTTGAA+TGG + chr8_3:25334152-25334171 Msa1283720:three_prime_UTR 35.0%
GTAAGGACTTGAAAAATGCA+GGG + chr8_3:25334175-25334194 Msa1283720:three_prime_UTR 35.0%
TTGGAGCCTGAAATTCTAAA+TGG - chr8_3:25334209-25334228 Msa1283730:three_prime_UTR 35.0%
AACATATGAACCATGATACG+TGG + chr8_3:25334477-25334496 Msa1283720:three_prime_UTR 35.0%
! GTTTTACACCGTTTGAATCA+AGG - chr8_3:25334494-25334513 Msa1283730:CDS 35.0%
AAAAATCAAGCGGAACTAGA+TGG + chr8_3:25334590-25334609 Msa1283720:three_prime_UTR 35.0%
!!! GACAAAATTTTCAGGTAGCT+TGG - chr8_3:25334800-25334819 Msa1283730:intron 35.0%
ATTTGAATCTTGCAGCATTG+GGG + chr8_3:25334875-25334894 Msa1283720:intron 35.0%
TATCGAATTAGGAGTAGGAA+GGG + chr8_3:25334901-25334920 Msa1283720:intron 35.0%
ATATCGAATTAGGAGTAGGA+AGG + chr8_3:25334902-25334921 Msa1283720:intron 35.0%
TCCTACTCCTAATTCGATAT+TGG - chr8_3:25334902-25334921 Msa1283730:intron 35.0%
CCTACTCCTAATTCGATATT+GGG - chr8_3:25334903-25334922 Msa1283730:intron 35.0%
TACAACTAGAGCAATACATG+GGG + chr8_3:25334973-25334992 Msa1283720:intron 35.0%
GATACAACTAGAGCAATACA+TGG + chr8_3:25334975-25334994 Msa1283720:intron 35.0%
AATTGCACTCAAATCCCATA+AGG - chr8_3:25335137-25335156 Msa1283730:intron 35.0%
! ACATCTCTCTACTTCTAGAA+AGG - chr8_3:25335174-25335193 Msa1283730:intron 35.0%
! TCTACTTCTAGAAAGGATCT+TGG - chr8_3:25335181-25335200 Msa1283730:intron 35.0%
! CCTTCATTTGTCTAAAATGG+TGG + chr8_3:25335618-25335637 Msa1283720:three_prime_UTR 35.0%
! CCACCATTTTAGACAAATGA+AGG - chr8_3:25335615-25335634 Msa1283730:CDS 35.0%
!! AGATGGTAGAAAGTACCTTA+GGG + chr8_3:25335666-25335685 Msa1283720:intron 35.0%
!! AAGATGGTAGAAAGTACCTT+AGG + chr8_3:25335667-25335686 Msa1283720:intron 35.0%
! AAGGTACTTTCTACCATCTT+AGG - chr8_3:25335667-25335686 Msa1283730:intron 35.0%
TTACAAACCTGGTCAAGTTA+GGG - chr8_3:25335832-25335851 Msa1283730:intron 35.0%
CATTTGTACCTGAAACATTG+GGG + chr8_3:25335891-25335910 Msa1283720:intron 35.0%
AGAATCGTTCAAGAAAACCT+AGG - chr8_3:25336011-25336030 Msa1283730:intron 35.0%
GAAAACCTAGGTTTGATTCT+TGG - chr8_3:25336023-25336042 Msa1283730:intron 35.0%
AAACCCAAAGGACAAAACAA+AGG - chr8_3:25336211-25336230 Msa1283730:intron 35.0%
!!! ACACCTTTGTTTTGTCCTTT+GGG + chr8_3:25336217-25336236 None:intergenic 35.0%
!!! TACACCTTTGTTTTGTCCTT+TGG + chr8_3:25336218-25336237 None:intergenic 35.0%
AGTGGATGATATCCTACTAA+AGG - chr8_3:25336259-25336278 Msa1283730:intron 35.0%
!! AGCAAGAAAGTACTACTCTA+AGG + chr8_3:25336311-25336330 None:intergenic 35.0%
!! CTATATATGTTTGGTGATGC+AGG - chr8_3:25336531-25336550 Msa1283730:CDS 35.0%
AAGTAGGGACTAAACTTACA+AGG + chr8_3:25337153-25337172 None:intergenic 35.0%
! TATCTTGGACACACATTTTC+TGG - chr8_3:25337331-25337350 Msa1283730:CDS 35.0%
! ATCTTGGACACACATTTTCT+GGG - chr8_3:25337332-25337351 Msa1283730:CDS 35.0%
TTTGACAGCCTCCTAATAAA+TGG - chr8_3:25337414-25337433 Msa1283730:intron 35.0%
ACACCACCATTCCATTTATT+AGG + chr8_3:25337428-25337447 None:intergenic 35.0%
! AATAAATGGAATGGTGGTGT+TGG - chr8_3:25337428-25337447 Msa1283730:intron 35.0%
! ATAAATGGAATGGTGGTGTT+GGG - chr8_3:25337429-25337448 Msa1283730:intron 35.0%
CAATTAAACCTGGATATCAG+GGG + chr8_3:25337498-25337517 None:intergenic 35.0%
GCAATTAAACCTGGATATCA+GGG + chr8_3:25337499-25337518 None:intergenic 35.0%
!! GTTGCTATGGCTTTATTCAT+GGG + chr8_3:25337653-25337672 None:intergenic 35.0%
!! AGTTGCTATGGCTTTATTCA+TGG + chr8_3:25337654-25337673 None:intergenic 35.0%
ACAATCATTGATCTGAGTGA+AGG - chr8_3:25337845-25337864 Msa1283730:intron 35.0%
! AAATTTTCAGTGCCAAGAAC+TGG + chr8_3:25338100-25338119 None:intergenic 35.0%
GGTAAGGACTTGAAAAATGC+AGG + chr8_3:25334176-25334195 Msa1283720:three_prime_UTR 40.0%
!! CAAGTTCTGAGCAAAAGGTA+AGG + chr8_3:25334192-25334211 Msa1283720:three_prime_UTR 40.0%
! CTTACCTTTTGCTCAGAACT+TGG - chr8_3:25334190-25334209 Msa1283730:three_prime_UTR 40.0%
TACTATCGCAAAAACCCCAA+AGG - chr8_3:25334275-25334294 Msa1283730:three_prime_UTR 40.0%
ATCATCAAGTCCACGTATCA+TGG - chr8_3:25334464-25334483 Msa1283730:exon 40.0%
CCCAATATCGAATTAGGAGT+AGG + chr8_3:25334906-25334925 Msa1283720:intron 40.0%
GGAAGTCCCAATATCGAATT+AGG + chr8_3:25334912-25334931 Msa1283720:intron 40.0%
!! AACCAATAATTGTGGTGGGT+GGG + chr8_3:25335112-25335131 Msa1283720:intron 40.0%
! ACTTCTAGAAAGGATCTTGG+AGG - chr8_3:25335184-25335203 Msa1283730:intron 40.0%
! GGTGGGACATTTTTCCATTT+GGG + chr8_3:25335600-25335619 Msa1283720:three_prime_UTR 40.0%
! TGGTGGGACATTTTTCCATT+TGG + chr8_3:25335601-25335620 Msa1283720:three_prime_UTR 40.0%
AATGAAGGAAGGTGACAACT+TGG - chr8_3:25335630-25335649 Msa1283730:intron 40.0%
GTTACAAACCTGGTCAAGTT+AGG - chr8_3:25335831-25335850 Msa1283730:intron 40.0%
! TGCCATTTAGGCACTTGAAA+AGG - chr8_3:25335915-25335934 Msa1283730:intron 40.0%
!!! CACCTTTTCAAGTGCCTAAA+TGG + chr8_3:25335920-25335939 Msa1283720:three_prime_UTR 40.0%
AGCCATACTAGAAAACCCAA+AGG - chr8_3:25336199-25336218 Msa1283730:intron 40.0%
!! GTCCTTTGGGTTTTCTAGTA+TGG + chr8_3:25336204-25336223 None:intergenic 40.0%
CTGTCACATTGGCGTAAATA+AGG + chr8_3:25336241-25336260 None:intergenic 40.0%
TATTTACGCCAATGTGACAG+TGG - chr8_3:25336241-25336260 Msa1283730:intron 40.0%
GATATCATCCACTGTCACAT+TGG + chr8_3:25336252-25336271 None:intergenic 40.0%
GTAGTACTTTCTTGCTTGTG+AGG - chr8_3:25336315-25336334 Msa1283730:CDS 40.0%
TGAAAGACATGGAATCTGTG+AGG + chr8_3:25336586-25336605 None:intergenic 40.0%
CAGCCTCCTAATAAATGGAA+TGG - chr8_3:25337419-25337438 Msa1283730:intron 40.0%
CCACCATTCCATTTATTAGG+AGG + chr8_3:25337425-25337444 None:intergenic 40.0%
CCTCCTAATAAATGGAATGG+TGG - chr8_3:25337422-25337441 Msa1283730:intron 40.0%
! TGGTGTTGGGTACATATCAA+AGG - chr8_3:25337442-25337461 Msa1283730:intron 40.0%
GGCAATTAAACCTGGATATC+AGG + chr8_3:25337500-25337519 None:intergenic 40.0%
!! TAGGTGGTGGTGATTTTTCT+TGG + chr8_3:25337572-25337591 None:intergenic 40.0%
TTGCTTCCATTTGCCAACAA+AGG - chr8_3:25337891-25337910 Msa1283730:intron 40.0%
TGCTTCCATTTGCCAACAAA+GGG - chr8_3:25337892-25337911 Msa1283730:intron 40.0%
ATACGAATTTGCCCCTTTGT+TGG + chr8_3:25337907-25337926 None:intergenic 40.0%
ATACACAAACTTGCCAAGTC+AGG - chr8_3:25338074-25338093 Msa1283730:five_prime_UTR 40.0%
GCAATTGCTCTTGTAGCTGT+TGG - chr8_3:25334233-25334252 Msa1283730:three_prime_UTR 45.0%
! TCTTGTAGCTGTTGGCCTAA+CGG - chr8_3:25334241-25334260 Msa1283730:three_prime_UTR 45.0%
TCGATATTGGGACTTCCTGT+TGG - chr8_3:25334915-25334934 Msa1283730:intron 45.0%
! CAACCAATAATTGTGGTGGG+TGG + chr8_3:25335113-25335132 Msa1283720:intron 45.0%
AGCAGAGGAAGAGATAGTCA+AGG - chr8_3:25335364-25335383 Msa1283730:intron 45.0%
TGCAAGCAGATGTACCCAAA+TGG - chr8_3:25335583-25335602 Msa1283730:CDS 45.0%
!! AATGGATTGCTGGAGCTTGT+AGG - chr8_3:25335799-25335818 Msa1283730:intron 45.0%
! ATGGATTGCTGGAGCTTGTA+GGG - chr8_3:25335800-25335819 Msa1283730:intron 45.0%
GGACGTTTCAGTTACAAACC+TGG - chr8_3:25335821-25335840 Msa1283730:intron 45.0%
! AGGGCATTTGGAATGATTGC+TGG - chr8_3:25335851-25335870 Msa1283730:intron 45.0%
! GCATTTGGAATGATTGCTGG+AGG - chr8_3:25335854-25335873 Msa1283730:intron 45.0%
AGGCATTACCCCAATGTTTC+AGG - chr8_3:25335880-25335899 Msa1283730:intron 45.0%
!! ATGGCTTTATTCATGGGTGG+TGG + chr8_3:25337647-25337666 None:intergenic 45.0%
!! GCTATGGCTTTATTCATGGG+TGG + chr8_3:25337650-25337669 None:intergenic 45.0%
TGCTGCAAGATGAGTTGCTA+TGG + chr8_3:25337666-25337685 None:intergenic 45.0%
GCTTCCATTTGCCAACAAAG+GGG - chr8_3:25337893-25337912 Msa1283730:intron 45.0%
TTTGCCCCTTTGTTGGCAAA+TGG + chr8_3:25337900-25337919 None:intergenic 45.0%
!!! TTTTTTTCAAATATTTATTA+AGG + chr8_3:25335302-25335321 Msa1283720:intron 5.0%
!! AAATAATTATAATATGAATT+CGG + chr8_3:25336087-25336106 Msa1283720:three_prime_UTR 5.0%
!!! ATAAGTAAATTTTTTTTTAT+AGG - chr8_3:25337108-25337127 Msa1283730:intron 5.0%
!!! TAAGTAAATTTTTTTTTATA+GGG - chr8_3:25337109-25337128 Msa1283730:intron 5.0%
!! TAATAATTTAAAAACAAAAA+TGG - chr8_3:25337593-25337612 Msa1283730:intron 5.0%
!! GGCTCCAAGTTCTGAGCAAA+AGG + chr8_3:25334197-25334216 Msa1283720:three_prime_UTR 50.0%
CACCCACCCACCACAATTAT+TGG - chr8_3:25335107-25335126 Msa1283730:intron 50.0%
AAGTACCTTAGGGCCCTTCA+CGG + chr8_3:25335656-25335675 Msa1283720:intron 50.0%
CCAAATGCCCTAACTTGACC+AGG + chr8_3:25335842-25335861 Msa1283720:intron 50.0%
! CCTGGTCAAGTTAGGGCATT+TGG - chr8_3:25335839-25335858 Msa1283730:intron 50.0%
GGAATGATTGCTGGAGGTTC+AGG - chr8_3:25335860-25335879 Msa1283730:intron 50.0%
TAAACCTGGATATCAGGGGC+AGG + chr8_3:25337494-25337513 None:intergenic 50.0%
GCAACTCATCTTGCAGCACT+TGG - chr8_3:25337668-25337687 Msa1283730:intron 50.0%
! TGCAGCATTGGGGCTGATCA+TGG + chr8_3:25334865-25334884 Msa1283720:intron 55.0%
AGGTGACAACTTGGCCGTGA+AGG - chr8_3:25335639-25335658 Msa1283730:intron 55.0%
GGTGACAACTTGGCCGTGAA+GGG - chr8_3:25335640-25335659 Msa1283730:intron 55.0%
TGCCAAGTCAGGCCAGTTCT+TGG - chr8_3:25338085-25338104 Msa1283730:five_prime_UTR 55.0%
! TGCCAAGAACTGGCCTGACT+TGG + chr8_3:25338090-25338109 None:intergenic 55.0%
TGGATATCAGGGGCAGGTGC+TGG + chr8_3:25337488-25337507 None:intergenic 60.0%
AGCACCTGCCCCTGATATCC+AGG - chr8_3:25337487-25337506 Msa1283730:intron 60.0%
CTTGGCCGTGAAGGGCCCTA+AGG - chr8_3:25335648-25335667 Msa1283730:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 25334079 25338120 25334079 ID=Msa1283730;Name=Msa1283730
chr8_3 mRNA 25334079 25338120 25334079 ID=Msa1283730-mRNA-1;Parent=Msa1283730;Name=Msa1283730-mRNA-1;_AED=0.10;_eAED=0.10;_QI=58|1|1|1|1|1|9|399|295
chr8_3 exon 25334079 25334570 25334079 ID=Msa1283730-mRNA-1:exon:8920;Parent=Msa1283730-mRNA-1
chr8_3 exon 25334691 25334777 25334691 ID=Msa1283730-mRNA-1:exon:8919;Parent=Msa1283730-mRNA-1
chr8_3 exon 25335581 25335646 25335581 ID=Msa1283730-mRNA-1:exon:8918;Parent=Msa1283730-mRNA-1
chr8_3 exon 25335919 25336008 25335919 ID=Msa1283730-mRNA-1:exon:8917;Parent=Msa1283730-mRNA-1
chr8_3 exon 25336298 25336378 25336298 ID=Msa1283730-mRNA-1:exon:8916;Parent=Msa1283730-mRNA-1
chr8_3 exon 25336530 25336607 25336530 ID=Msa1283730-mRNA-1:exon:8915;Parent=Msa1283730-mRNA-1
chr8_3 exon 25336740 25336830 25336740 ID=Msa1283730-mRNA-1:exon:8914;Parent=Msa1283730-mRNA-1
chr8_3 exon 25337244 25337385 25337244 ID=Msa1283730-mRNA-1:exon:8913;Parent=Msa1283730-mRNA-1
chr8_3 exon 25337903 25338120 25337903 ID=Msa1283730-mRNA-1:exon:8912;Parent=Msa1283730-mRNA-1
chr8_3 five_prime_UTR 25338063 25338120 25338063 ID=Msa1283730-mRNA-1:five_prime_utr;Parent=Msa1283730-mRNA-1
chr8_3 CDS 25337903 25338062 25337903 ID=Msa1283730-mRNA-1:cds;Parent=Msa1283730-mRNA-1
chr8_3 CDS 25337244 25337385 25337244 ID=Msa1283730-mRNA-1:cds;Parent=Msa1283730-mRNA-1
chr8_3 CDS 25336740 25336830 25336740 ID=Msa1283730-mRNA-1:cds;Parent=Msa1283730-mRNA-1
chr8_3 CDS 25336530 25336607 25336530 ID=Msa1283730-mRNA-1:cds;Parent=Msa1283730-mRNA-1
chr8_3 CDS 25336298 25336378 25336298 ID=Msa1283730-mRNA-1:cds;Parent=Msa1283730-mRNA-1
chr8_3 CDS 25335919 25336008 25335919 ID=Msa1283730-mRNA-1:cds;Parent=Msa1283730-mRNA-1
chr8_3 CDS 25335581 25335646 25335581 ID=Msa1283730-mRNA-1:cds;Parent=Msa1283730-mRNA-1
chr8_3 CDS 25334691 25334777 25334691 ID=Msa1283730-mRNA-1:cds;Parent=Msa1283730-mRNA-1
chr8_3 CDS 25334478 25334570 25334478 ID=Msa1283730-mRNA-1:cds;Parent=Msa1283730-mRNA-1
chr8_3 three_prime_UTR 25334079 25334477 25334079 ID=Msa1283730-mRNA-1:three_prime_utr;Parent=Msa1283730-mRNA-1
Gene Sequence

>Msa1283730

ATGTTGGAGTTACCATTCAACATGACACTTAGCTTCCCTGCATTTTTCAAGTCCTTACCTTTTGCTCAGAACTTGGAGCCTGAAATTCTAAATGGTGCAATTGCTCTTGTAGCTGTTGGCCTAACGGTTGCTTATATTTACTATCGCAAAAACCCCAAAGGTAGCTTGGATCCCAAAAATTTCAAAGAATTTAAACTTGTAAAGAAGACCATGATCAGCCCCAATGCTGCAAGATTCAAATTTGCCCTTCCTACTCCTAATTCGATATTGGGACTTCCTGTTGGAAAAAACATACTTGTCAGAGGAAGAGATAGTCAAGGAGAAGAAGTTATGAGATCATATACTCCAATCACATTGGATTCAGATATTGGCTATTTTGAATTAGTTGTGAAGATGTACCCAAATGGAAAAATGTCCCACCATTTTAGACAAATGAAGGAAGGTGACAACTTGGCCGTGAAGGGCCCTAAGGGACGTTTCAGTTACAAACCTGGTCAAGTTAGGGCATTTGGAATGATTGCTGGAGGTTCAGGCATTACCCCAATGTTTCAGATCATAAGAGCCATACTAGAAAACCCAAAGGACAAAACAAAGGTGTACCTTATTTACGCCAATGTGACAGTGGATGATATCCTACTAAAGGAAGAACTAGATCGCTTTGCAGACAAATTTCCTCACAGATTCCATGTCTTTCACGTTCTTAATAAGCCTCCTAATAAATGGAATGGTGGTGTTGGGTACATATCAAAGGAGATGATTGAATCTCATTGTCCAGCACCTGCCCCTGATATCCAGATACTTAGATGTGGTCCACCACCCATGAATAAAGCCATAGCAACTCATCTTGCAGCACTTGGATACACATCAAACATGCAGTTCGAGTTCTAA
Protein sequence

>Msa1283730

MLELPFNMTLSFPAFFKSLPFAQNLEPEILNGAIALVAVGLTVAYIYYRKNPKGSLDPKNFKEFKLVKKTMISPNAARFKFALPTPNSILGLPVGKNILVRGRDSQGEEVMRSYTPITLDSDIGYFELVVKMYPNGKMSHHFRQMKEGDNLAVKGPKGRFSYKPGQVRAFGMIAGGSGITPMFQIIRAILENPKDKTKVYLIYANVTVDDILLKEELDRFADKFPHRFHVFHVLNKPPNKWNGGVGYISKEMIESHCPAPAPDIQILRCGPPPMNKAIATHLAALGYTSNMQFEF*