AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0053850


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0053850 MtrunA17_Chr1g0150751 85.714 217 29 2 1 216 24 239 6.60e-76 228
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCGCCTTCTTCGGGTTC+AGG 0.278379 1_2:+8357557 Msa0053850:CDS
TGAGGCATAATAATAGTAAT+AGG 0.283637 1_2:-8357630 None:intergenic
TTTGAAATCTTCGCCTTCTT+CGG 0.306660 1_2:+8357550 Msa0053850:CDS
GGTGGTCCACCAATTACTTT+AGG 0.326407 1_2:-8357590 None:intergenic
CAAGGACAACCTAAAGTAAT+TGG 0.337399 1_2:+8357581 Msa0053850:CDS
TTGAAATCTTCGCCTTCTTC+GGG 0.338296 1_2:+8357551 Msa0053850:CDS
GAATTTGATGAAGCAAGTTT+AGG 0.338870 1_2:-8357209 None:intergenic
AGTCCTTCGTCACATTCTTC+CGG 0.348481 1_2:+8357509 Msa0053850:CDS
GTCCTTCGTCACATTCTTCC+GGG 0.349605 1_2:+8357510 Msa0053850:CDS
TTTGATGATGTGAGTGGAAA+TGG 0.378894 1_2:-8357392 None:intergenic
TTCATCAGATATTCTTTGCT+TGG 0.384427 1_2:+8357708 Msa0053850:CDS
TAATAATAGTAATAGGGATA+TGG 0.384788 1_2:-8357623 None:intergenic
TGTGTTAGGAGTGGAGTAGA+TGG 0.387212 1_2:-8357329 None:intergenic
TTTAGAAGTGAAGGAGGAGG+AGG 0.388578 1_2:-8357419 None:intergenic
GTTAATAGTGGAGGAGGTGG+AGG 0.430557 1_2:-8357356 None:intergenic
TGGGGTGTTATTAGTGGAAA+AGG 0.432885 1_2:-8357302 None:intergenic
TTGATGATGTGAGTGGAAAT+GGG 0.439050 1_2:-8357391 None:intergenic
CCTTCTTCGGGTTCAGGTCA+AGG 0.439335 1_2:+8357563 Msa0053850:CDS
GGTGAATATGATGTTAATAG+TGG 0.445887 1_2:-8357368 None:intergenic
GAGGCATAATAATAGTAATA+GGG 0.446723 1_2:-8357629 None:intergenic
AGTGGAGTAGATGGAGGGTG+GGG 0.452281 1_2:-8357320 None:intergenic
GGAGGGTGGGGTGTTATTAG+TGG 0.452753 1_2:-8357308 None:intergenic
ATGCATAATAAACCACTTGA+AGG 0.460999 1_2:-8357143 None:intergenic
GGGGTGTTATTAGTGGAAAA+GGG 0.461571 1_2:-8357301 None:intergenic
GATGTTAATAGTGGAGGAGG+TGG 0.484497 1_2:-8357359 None:intergenic
AAAGAGAACATTGGAGATTG+AGG 0.496507 1_2:+8357103 Msa0053850:CDS
GGGTGTTATTAGTGGAAAAG+GGG 0.496788 1_2:-8357300 None:intergenic
GAGTGGAGTAGATGGAGGGT+GGG 0.505478 1_2:-8357321 None:intergenic
GAAGAATGTGACGAAGGACT+TGG 0.508990 1_2:-8357506 None:intergenic
GGCGAAGATTTCAAAGGTGG+TGG 0.511485 1_2:-8357542 None:intergenic
GGAGTGGAGTAGATGGAGGG+TGG 0.519473 1_2:-8357322 None:intergenic
TTTGTTTGCAAAGAGAACAT+TGG 0.519949 1_2:+8357094 Msa0053850:CDS
GGTGGTGGTGAACAAATTGT+AGG 0.526245 1_2:-8357185 None:intergenic
GTCAACAAAGGAGGAGGTGA+AGG 0.526752 1_2:-8357266 None:intergenic
TTTGATGAAGCAAGTTTAGG+AGG 0.539473 1_2:-8357206 None:intergenic
GATGAAGCAAGTTTAGGAGG+TGG 0.545014 1_2:-8357203 None:intergenic
GAAGATTTCAAAGGTGGTGG+AGG 0.554576 1_2:-8357539 None:intergenic
GAATGTGACGAAGGACTTGG+TGG 0.555897 1_2:-8357503 None:intergenic
CCTTGACCTGAACCCGAAGA+AGG 0.567300 1_2:-8357563 None:intergenic
TATGATGTTAATAGTGGAGG+AGG 0.568744 1_2:-8357362 None:intergenic
GGACAACCTAAAGTAATTGG+TGG 0.583322 1_2:+8357584 Msa0053850:CDS
GAAGCAAGTTTAGGAGGTGG+TGG 0.584772 1_2:-8357200 None:intergenic
TGATGATGTGAGTGGAAATG+GGG 0.585770 1_2:-8357390 None:intergenic
AAAGTTGAAGAGCCTTCAAG+TGG 0.589794 1_2:+8357131 Msa0053850:CDS
AGGTGGTGGAGGAGTTTCCC+CGG 0.592101 1_2:-8357528 None:intergenic
GTGGTGGAAGACTTAGTGAT+GGG 0.594811 1_2:-8357454 None:intergenic
GTTAGGAGTGGAGTAGATGG+AGG 0.597422 1_2:-8357326 None:intergenic
TTAGGAGTGGAGTAGATGGA+GGG 0.598597 1_2:-8357325 None:intergenic
TTTGGTGTCAACAAAGGAGG+AGG 0.602071 1_2:-8357272 None:intergenic
GGTGCATTTGGTGTCAACAA+AGG 0.602133 1_2:-8357278 None:intergenic
GAAGGACTTGGTGGAGATGG+TGG 0.605778 1_2:-8357494 None:intergenic
GGTGGAAGACTTAGTGATGG+GGG 0.608316 1_2:-8357452 None:intergenic
GACGAAGGACTTGGTGGAGA+TGG 0.610720 1_2:-8357497 None:intergenic
GAATATGATGTTAATAGTGG+AGG 0.611804 1_2:-8357365 None:intergenic
GGTAGAGTCAACAATGGTGG+TGG 0.613265 1_2:-8357470 None:intergenic
GGACTTGGTGGAGATGGTGG+CGG 0.623426 1_2:-8357491 None:intergenic
GGAGAATTTGATGATGTGAG+TGG 0.631413 1_2:-8357398 None:intergenic
GCATTTGGTGTCAACAAAGG+AGG 0.639121 1_2:-8357275 None:intergenic
TCCTTCGTCACATTCTTCCG+GGG 0.641768 1_2:+8357511 Msa0053850:CDS
TGGTGGAAGACTTAGTGATG+GGG 0.649103 1_2:-8357453 None:intergenic
GAAGAAGGCGAAGATTTCAA+AGG 0.649156 1_2:-8357548 None:intergenic
GAAGGCGAAGATTTCAAAGG+TGG 0.657373 1_2:-8357545 None:intergenic
GGCGGTAGAGTCAACAATGG+TGG 0.660549 1_2:-8357473 None:intergenic
GGTGGTGGAAGACTTAGTGA+TGG 0.666350 1_2:-8357455 None:intergenic
GGTGTTATTAGTGGAAAAGG+GGG 0.675151 1_2:-8357299 None:intergenic
TCCCCGGAAGAATGTGACGA+AGG 0.679405 1_2:-8357512 None:intergenic
GATGATGTGAGTGGAAATGG+GGG 0.687997 1_2:-8357389 None:intergenic
GGTGGCGGTAGAGTCAACAA+TGG 0.730976 1_2:-8357476 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATAGTAATAGGGATA+TGG - chr1_2:8357626-8357645 None:intergenic 20.0%
! GAGGCATAATAATAGTAATA+GGG - chr1_2:8357632-8357651 None:intergenic 25.0%
! TGAGGCATAATAATAGTAAT+AGG - chr1_2:8357633-8357652 None:intergenic 25.0%
TTTGTTTGCAAAGAGAACAT+TGG + chr1_2:8357094-8357113 Msa0053850:CDS 30.0%
ATGCATAATAAACCACTTGA+AGG - chr1_2:8357146-8357165 None:intergenic 30.0%
GAATTTGATGAAGCAAGTTT+AGG - chr1_2:8357212-8357231 None:intergenic 30.0%
GAATATGATGTTAATAGTGG+AGG - chr1_2:8357368-8357387 None:intergenic 30.0%
GGTGAATATGATGTTAATAG+TGG - chr1_2:8357371-8357390 None:intergenic 30.0%
!! TATGATTTTAGAAGTGAAGG+AGG - chr1_2:8357428-8357447 None:intergenic 30.0%
!! GGATATGATTTTAGAAGTGA+AGG - chr1_2:8357431-8357450 None:intergenic 30.0%
TTCATCAGATATTCTTTGCT+TGG + chr1_2:8357708-8357727 Msa0053850:CDS 30.0%
AAAGAGAACATTGGAGATTG+AGG + chr1_2:8357103-8357122 Msa0053850:CDS 35.0%
TTTGATGAAGCAAGTTTAGG+AGG - chr1_2:8357209-8357228 None:intergenic 35.0%
! TATGATGTTAATAGTGGAGG+AGG - chr1_2:8357365-8357384 None:intergenic 35.0%
!! TTGATGATGTGAGTGGAAAT+GGG - chr1_2:8357394-8357413 None:intergenic 35.0%
!! TTTGATGATGTGAGTGGAAA+TGG - chr1_2:8357395-8357414 None:intergenic 35.0%
! TTTGAAATCTTCGCCTTCTT+CGG + chr1_2:8357550-8357569 Msa0053850:CDS 35.0%
CAAGGACAACCTAAAGTAAT+TGG + chr1_2:8357581-8357600 Msa0053850:CDS 35.0%
!! TAGGGATATGGATAGTTTTG+TGG - chr1_2:8357614-8357633 None:intergenic 35.0%
AGAAAAATGAGATGCACTTG+AGG - chr1_2:8357651-8357670 None:intergenic 35.0%
AAAGTTGAAGAGCCTTCAAG+TGG + chr1_2:8357131-8357150 Msa0053850:CDS 40.0%
! AGGTGAAGGATTTTGTGTTG+GGG - chr1_2:8357255-8357274 None:intergenic 40.0%
! GAGGTGAAGGATTTTGTGTT+GGG - chr1_2:8357256-8357275 None:intergenic 40.0%
!! GGTGTTATTAGTGGAAAAGG+GGG - chr1_2:8357302-8357321 None:intergenic 40.0%
!! GGGTGTTATTAGTGGAAAAG+GGG - chr1_2:8357303-8357322 None:intergenic 40.0%
!! GGGGTGTTATTAGTGGAAAA+GGG - chr1_2:8357304-8357323 None:intergenic 40.0%
!! TGGGGTGTTATTAGTGGAAA+AGG - chr1_2:8357305-8357324 None:intergenic 40.0%
! TGATGATGTGAGTGGAAATG+GGG - chr1_2:8357393-8357412 None:intergenic 40.0%
!! GGAGAATTTGATGATGTGAG+TGG - chr1_2:8357401-8357420 None:intergenic 40.0%
!! GATTTTAGAAGTGAAGGAGG+AGG - chr1_2:8357425-8357444 None:intergenic 40.0%
GAAGAAGGCGAAGATTTCAA+AGG - chr1_2:8357551-8357570 None:intergenic 40.0%
TTGAAATCTTCGCCTTCTTC+GGG + chr1_2:8357551-8357570 Msa0053850:CDS 40.0%
GGACAACCTAAAGTAATTGG+TGG + chr1_2:8357584-8357603 Msa0053850:CDS 40.0%
!!! GGATATGGATAGTTTTGTGG+TGG - chr1_2:8357611-8357630 None:intergenic 40.0%
! GGTGGTGGTGAACAAATTGT+AGG - chr1_2:8357188-8357207 None:intergenic 45.0%
GATGAAGCAAGTTTAGGAGG+TGG - chr1_2:8357206-8357225 None:intergenic 45.0%
! GGTGAAGGATTTTGTGTTGG+GGG - chr1_2:8357254-8357273 None:intergenic 45.0%
! GGAGGTGAAGGATTTTGTGT+TGG - chr1_2:8357257-8357276 None:intergenic 45.0%
!! TTTGGTGTCAACAAAGGAGG+AGG - chr1_2:8357275-8357294 None:intergenic 45.0%
!! GCATTTGGTGTCAACAAAGG+AGG - chr1_2:8357278-8357297 None:intergenic 45.0%
!! GGTGCATTTGGTGTCAACAA+AGG - chr1_2:8357281-8357300 None:intergenic 45.0%
TTAGGAGTGGAGTAGATGGA+GGG - chr1_2:8357328-8357347 None:intergenic 45.0%
TGTGTTAGGAGTGGAGTAGA+TGG - chr1_2:8357332-8357351 None:intergenic 45.0%
! GGAGGATTTTGTGTTAGGAG+TGG - chr1_2:8357341-8357360 None:intergenic 45.0%
! GAGGTGGAGGATTTTGTGTT+AGG - chr1_2:8357346-8357365 None:intergenic 45.0%
! GATGTTAATAGTGGAGGAGG+TGG - chr1_2:8357362-8357381 None:intergenic 45.0%
GATGATGTGAGTGGAAATGG+GGG - chr1_2:8357392-8357411 None:intergenic 45.0%
TTTAGAAGTGAAGGAGGAGG+AGG - chr1_2:8357422-8357441 None:intergenic 45.0%
TGGTGGAAGACTTAGTGATG+GGG - chr1_2:8357456-8357475 None:intergenic 45.0%
! GTGGTGGAAGACTTAGTGAT+GGG - chr1_2:8357457-8357476 None:intergenic 45.0%
GAAGAATGTGACGAAGGACT+TGG - chr1_2:8357509-8357528 None:intergenic 45.0%
AGTCCTTCGTCACATTCTTC+CGG + chr1_2:8357509-8357528 Msa0053850:CDS 45.0%
! GAAGATTTCAAAGGTGGTGG+AGG - chr1_2:8357542-8357561 None:intergenic 45.0%
GAAGGCGAAGATTTCAAAGG+TGG - chr1_2:8357548-8357567 None:intergenic 45.0%
GGTGGTCCACCAATTACTTT+AGG - chr1_2:8357593-8357612 None:intergenic 45.0%
GAAGCAAGTTTAGGAGGTGG+TGG - chr1_2:8357203-8357222 None:intergenic 50.0%
! TTTTGTGTTGGGGGTGAAGG+AGG - chr1_2:8357245-8357264 None:intergenic 50.0%
! GGATTTTGTGTTGGGGGTGA+AGG - chr1_2:8357248-8357267 None:intergenic 50.0%
GTCAACAAAGGAGGAGGTGA+AGG - chr1_2:8357269-8357288 None:intergenic 50.0%
! AGTGGAAAAGGGGGTGCATT+TGG - chr1_2:8357293-8357312 None:intergenic 50.0%
GTTAGGAGTGGAGTAGATGG+AGG - chr1_2:8357329-8357348 None:intergenic 50.0%
! GTTAATAGTGGAGGAGGTGG+AGG - chr1_2:8357359-8357378 None:intergenic 50.0%
GGTGGAAGACTTAGTGATGG+GGG - chr1_2:8357455-8357474 None:intergenic 50.0%
!! GGTGGTGGAAGACTTAGTGA+TGG - chr1_2:8357458-8357477 None:intergenic 50.0%
GGTAGAGTCAACAATGGTGG+TGG - chr1_2:8357473-8357492 None:intergenic 50.0%
GAATGTGACGAAGGACTTGG+TGG - chr1_2:8357506-8357525 None:intergenic 50.0%
GTCCTTCGTCACATTCTTCC+GGG + chr1_2:8357510-8357529 Msa0053850:CDS 50.0%
TCCTTCGTCACATTCTTCCG+GGG + chr1_2:8357511-8357530 Msa0053850:CDS 50.0%
GGCGAAGATTTCAAAGGTGG+TGG - chr1_2:8357545-8357564 None:intergenic 50.0%
!! GGAGGGTGGGGTGTTATTAG+TGG - chr1_2:8357311-8357330 None:intergenic 55.0%
AGTGGAGTAGATGGAGGGTG+GGG - chr1_2:8357323-8357342 None:intergenic 55.0%
GAGTGGAGTAGATGGAGGGT+GGG - chr1_2:8357324-8357343 None:intergenic 55.0%
! GGAAGACTTAGTGATGGGGG+AGG - chr1_2:8357452-8357471 None:intergenic 55.0%
GGCGGTAGAGTCAACAATGG+TGG - chr1_2:8357476-8357495 None:intergenic 55.0%
! GGTGGCGGTAGAGTCAACAA+TGG - chr1_2:8357479-8357498 None:intergenic 55.0%
!! GAAGGACTTGGTGGAGATGG+TGG - chr1_2:8357497-8357516 None:intergenic 55.0%
!! GACGAAGGACTTGGTGGAGA+TGG - chr1_2:8357500-8357519 None:intergenic 55.0%
TCCCCGGAAGAATGTGACGA+AGG - chr1_2:8357515-8357534 None:intergenic 55.0%
! TCTTCGCCTTCTTCGGGTTC+AGG + chr1_2:8357557-8357576 Msa0053850:CDS 55.0%
CCTTGACCTGAACCCGAAGA+AGG - chr1_2:8357566-8357585 None:intergenic 55.0%
! CCTTCTTCGGGTTCAGGTCA+AGG + chr1_2:8357563-8357582 Msa0053850:CDS 55.0%
GTGTTGGGGGTGAAGGAGGA+GGG - chr1_2:8357241-8357260 None:intergenic 60.0%
TGTGTTGGGGGTGAAGGAGG+AGG - chr1_2:8357242-8357261 None:intergenic 60.0%
GGAGTGGAGTAGATGGAGGG+TGG - chr1_2:8357325-8357344 None:intergenic 60.0%
!! GGACTTGGTGGAGATGGTGG+CGG - chr1_2:8357494-8357513 None:intergenic 60.0%
!! AGGTGGTGGAGGAGTTTCCC+CGG - chr1_2:8357531-8357550 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 8357083 8357733 8357083 ID=Msa0053850;Name=Msa0053850
chr1_2 mRNA 8357083 8357733 8357083 ID=Msa0053850-mRNA-1;Parent=Msa0053850;Name=Msa0053850-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|-1|0|1|-1|1|1|0|216
chr1_2 exon 8357083 8357733 8357083 ID=Msa0053850-mRNA-1:exon:2959;Parent=Msa0053850-mRNA-1
chr1_2 CDS 8357083 8357733 8357083 ID=Msa0053850-mRNA-1:cds;Parent=Msa0053850-mRNA-1
Gene Sequence

>Msa0053850

ATGTTGTTGGTTTTGTTTGCAAAGAGAACATTGGAGATTGAGGACAACAAAGTTGAAGAGCCTTCAAGTGGTTTATTATGCATTAGTGAATGTGTCACGTGTCCTACAATTTGTTCACCACCACCTCCTAAACTTGCTTCATCAAATTCAAATCACCCTCCTCCTTCACCCCCAACACAAAATCCTTCACCTCCTCCTTTGTTGACACCAAATGCACCCCCTTTTCCACTAATAACACCCCACCCTCCATCTACTCCACTCCTAACACAAAATCCTCCACCTCCTCCACTATTAACATCATATTCACCCCCATTTCCACTCACATCATCAAATTCTCCTCCTCCTCCTTCACTTCTAAAATCATATCCTCCCCCATCACTAAGTCTTCCACCACCATTGTTGACTCTACCGCCACCATCTCCACCAAGTCCTTCGTCACATTCTTCCGGGGAAACTCCTCCACCACCTTTGAAATCTTCGCCTTCTTCGGGTTCAGGTCAAGGACAACCTAAAGTAATTGGTGGACCACCACAAAACTATCCATATCCCTATTACTATTATTATGCCTCAAGTGCATCTCATTTTTCTAATCATGTTGTACTCTTCTTTATTGTACTTTTGTTCATTCATCAGATATTCTTTGCTTGGTAA

Protein sequence

>Msa0053850

MLLVLFAKRTLEIEDNKVEEPSSGLLCISECVTCPTICSPPPPKLASSNSNHPPPSPPTQNPSPPPLLTPNAPPFPLITPHPPSTPLLTQNPPPPPLLTSYSPPFPLTSSNSPPPPSLLKSYPPPSLSLPPPLLTLPPPSPPSPSSHSSGETPPPPLKSSPSSGSGQGQPKVIGGPPQNYPYPYYYYYASSASHFSNHVVLFFIVLLFIHQIFFAW*