AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1188170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1188170 MtrunA17_Chr8g0355241 87.119 295 8 1 1 265 1 295 0.0 518
Msa1188170 MtrunA17_Chr3g0118791 58.273 278 85 2 19 265 1 278 1.48e-116 335
Msa1188170 MtrunA17_Chr7g0254371 56.574 251 79 1 45 265 28 278 1.05e-104 305
Msa1188170 MtrunA17_Chr4g0063451 33.559 295 130 8 35 265 33 325 7.78e-45 153
Msa1188170 MtrunA17_Chr5g0424491 34.646 254 113 7 55 257 624 875 9.99e-35 132
Msa1188170 MtrunA17_Chr3g0115151 33.462 260 118 9 55 261 578 835 2.18e-31 122
Msa1188170 MtrunA17_Chr3g0115131 30.502 259 126 7 50 257 612 867 1.33e-29 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1188170 AT5G17770.1 57.769 251 76 1 45 265 31 281 5.00e-106 309
Msa1188170 AT5G20080.1 34.177 316 146 7 10 265 15 328 1.48e-47 161
Msa1188170 AT1G37130.1 31.274 259 126 7 56 263 657 914 4.88e-30 119
Msa1188170 AT1G77760.1 29.644 253 126 7 56 257 657 908 2.93e-28 114

Find 79 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTTCATTTGTCTAAAA+TGG 0.154232 8_1:+28242118 None:intergenic
TGTCTTTCACGTTCTTAATA+AGG 0.198260 8_1:-28240942 Msa1188170:intron
GGCAATTAAACCTGGATATC+AGG 0.207511 8_1:+28240592 Msa1188160:three_prime_UTR
ATTGCACCATTTAGAATTTC+AGG 0.220278 8_1:+28243523 None:intergenic
TCCTACTCCTAATTCGATAT+TGG 0.222016 8_1:-28242836 Msa1188170:CDS
ATCACATTGGATTCAGATAT+TGG 0.246714 8_1:-28242321 Msa1188170:CDS
ACACCACCATTCCATTTATT+AGG 0.270818 8_1:+28240664 Msa1188160:three_prime_UTR
GTTACAAACCTGGTCAAGTT+AGG 0.279037 8_1:-28241905 Msa1188170:CDS
TTTGACAGCCTCCTAATAAA+TGG 0.283114 8_1:-28240675 Msa1188170:intron
AGGCATTACCCCAATGTTTC+AGG 0.287455 8_1:-28241856 Msa1188170:intron
GGAAGTCCCAATATCGAATT+AGG 0.287589 8_1:+28242829 None:intergenic
CCTGGTCAAGTTAGGGCATT+TGG 0.298742 8_1:-28241897 Msa1188170:CDS
TTGGAGCCTGAAATTCTAAA+TGG 0.308092 8_1:-28243529 Msa1188170:CDS
GGAATGATTGCTGGAGGTTC+AGG 0.322357 8_1:-28241876 Msa1188170:CDS
TGCCAAGTCAGGCCAGTTCT+TGG 0.337644 8_1:-28240004 Msa1188170:three_prime_UTR
CCTACTCCTAATTCGATATT+GGG 0.343680 8_1:-28242835 Msa1188170:CDS
AATTTGAATCTTGCAGCATT+GGG 0.356810 8_1:+28242865 None:intergenic
AGTTGCTATGGCTTTATTCA+TGG 0.365210 8_1:+28240438 Msa1188160:three_prime_UTR
ATCACTATAATCATTCTATA+TGG 0.372899 8_1:-28240129 Msa1188170:three_prime_UTR
TTCATTTGTACCTGAAACAT+TGG 0.379448 8_1:+28241846 None:intergenic
TCATTTGTACCTGAAACATT+GGG 0.390761 8_1:+28241847 None:intergenic
GCAATTGCTCTTGTTGCTGT+TGG 0.390798 8_1:-28243505 Msa1188170:CDS
TGCTTCCATTTGTCAACAAA+GGG 0.401316 8_1:-28240197 Msa1188170:three_prime_UTR
AGGGCATTTGGAATGATTGC+TGG 0.412351 8_1:-28241885 Msa1188170:CDS
AAATTTGAATCTTGCAGCAT+TGG 0.413872 8_1:+28242864 None:intergenic
AGCACCTGCCCCTGATATCC+AGG 0.426304 8_1:-28240602 Msa1188170:intron
TTTGCCCCTTTGTTGACAAA+TGG 0.430112 8_1:+28240192 Msa1188160:three_prime_UTR
TGCAGCATTGGGGCTGATCA+TGG 0.437050 8_1:+28242876 None:intergenic
TGGATATCAGGGGCAGGTGC+TGG 0.440181 8_1:+28240604 Msa1188160:three_prime_UTR
ATATCTGAATCCAATGTGAT+TGG 0.441409 8_1:+28242324 None:intergenic
GGCTCCAAATTCTGAGCAAA+AGG 0.451991 8_1:+28243544 None:intergenic
CCAAATGCCCTAACTTGACC+AGG 0.467529 8_1:+28241897 None:intergenic
TCGATATTGGGACTTCCTGT+TGG 0.490436 8_1:-28242823 Msa1188170:CDS
TGCCAAGAACTGGCCTGACT+TGG 0.491460 8_1:+28240002 Msa1188160:three_prime_UTR
GCAACTCATCTTGCAGCACT+TGG 0.495897 8_1:-28240421 Msa1188170:CDS
AGGTGACACCTTGGCCGTGA+AGG 0.505334 8_1:-28242097 Msa1188170:CDS
GCAATTAAACCTGGATATCA+GGG 0.505823 8_1:+28240593 Msa1188160:three_prime_UTR
TTACAAACCTGGTCAAGTTA+GGG 0.508630 8_1:-28241904 Msa1188170:CDS
TTGCTTCCATTTGTCAACAA+AGG 0.509327 8_1:-28240198 Msa1188170:three_prime_UTR
AAGCTAAGTGTCATGTTGAA+TGG 0.510044 8_1:+28243589 None:intergenic
AGTAAATATAAGCAACCGTT+AGG 0.512828 8_1:+28243482 None:intergenic
ATACACAAACTTGCCAAGTC+AGG 0.512876 8_1:-28240015 Msa1188170:three_prime_UTR
TCTTGTTGCTGTTGGCCTAA+CGG 0.513537 8_1:-28243497 Msa1188170:CDS
TGCAAGCAGATGTACCCAAA+TGG 0.517984 8_1:-28242153 Msa1188170:intron
CCACCATTCCATTTATTAGG+AGG 0.525573 8_1:+28240667 Msa1188160:three_prime_UTR
ATGGCTTTATTCATGGGTGG+TGG 0.526824 8_1:+28240445 Msa1188160:three_prime_UTR
GGTGACACCTTGGCCGTGAA+GGG 0.528919 8_1:-28242096 Msa1188170:CDS
AATAAATGGAATGGTGGTGT+TGG 0.539466 8_1:-28240661 Msa1188170:CDS
AAGTACCTTAGGGCCCTTCA+CGG 0.543318 8_1:+28242083 None:intergenic
GTTGCTATGGCTTTATTCAT+GGG 0.544781 8_1:+28240439 Msa1188160:three_prime_UTR
CTTGGCCGTGAAGGGCCCTA+AGG 0.552829 8_1:-28242088 Msa1188170:intron
ATAAATGGAATGGTGGTGTT+GGG 0.556556 8_1:-28240660 Msa1188170:CDS
CTTAGGGCCCTTCACGGCCA+AGG 0.558481 8_1:+28242089 None:intergenic
TAATAATAACTACACCTTTG+GGG 0.566489 8_1:+28243449 None:intergenic
ATCATATACTCCAATCACAT+TGG 0.569714 8_1:-28242334 Msa1188170:CDS
CTAATTAATCAACAACACCT+TGG 0.571299 8_1:+28243640 None:intergenic
TGCTGCAAGATGAGTTGCTA+TGG 0.575868 8_1:+28240426 Msa1188160:three_prime_UTR
CAGCCTCCTAATAAATGGAA+TGG 0.578707 8_1:-28240670 Msa1188170:CDS
CAAATTCTGAGCAAAAGGTA+AGG 0.584509 8_1:+28243549 None:intergenic
TGATTATAGTGATTGAAAGA+AGG 0.587279 8_1:+28240139 Msa1188160:three_prime_UTR
TAAACCTGGATATCAGGGGC+AGG 0.589659 8_1:+28240598 Msa1188160:three_prime_UTR
ATATCGAATTAGGAGTAGGA+AGG 0.599298 8_1:+28242839 None:intergenic
CCCAATATCGAATTAGGAGT+AGG 0.601609 8_1:+28242835 None:intergenic
GCATTTGGAATGATTGCTGG+AGG 0.605362 8_1:-28241882 Msa1188170:CDS
TATCGAATTAGGAGTAGGAA+GGG 0.606000 8_1:+28242840 None:intergenic
GCTATGGCTTTATTCATGGG+TGG 0.610095 8_1:+28240442 Msa1188160:three_prime_UTR
CATTTGTACCTGAAACATTG+GGG 0.612740 8_1:+28241848 None:intergenic
TGGTGTTGGGTACATATCAA+AGG 0.615193 8_1:-28240647 Msa1188170:CDS
ACAATCATTGATCTGAGTGA+AGG 0.616710 8_1:-28240244 Msa1188170:three_prime_UTR
GGACGTTTCAGTTACAAACC+TGG 0.618497 8_1:-28241915 Msa1188170:CDS
TATTAAGAACGTGAAAGACA+TGG 0.621704 8_1:+28240945 Msa1188160:three_prime_UTR
AGCAGAGGAAGAGATAGTCA+AGG 0.623400 8_1:-28242372 Msa1188170:intron
AATGAAGGAAGGTGACACCT+TGG 0.626381 8_1:-28242106 Msa1188170:CDS
CCTCCTAATAAATGGAATGG+TGG 0.626581 8_1:-28240667 Msa1188170:CDS
ATTTGAATCTTGCAGCATTG+GGG 0.627950 8_1:+28242866 None:intergenic
AAATGTGTATTTATAAACCA+AGG 0.637836 8_1:-28243657 Msa1188170:five_prime_UTR
GCTTCCATTTGTCAACAAAG+GGG 0.670543 8_1:-28240196 Msa1188170:three_prime_UTR
CAATTAAACCTGGATATCAG+GGG 0.687288 8_1:+28240594 Msa1188160:three_prime_UTR
TGAAAGACATGGAATCTGTG+AGG 0.721466 8_1:+28240956 Msa1188160:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAATTCTTTGAAATTTTT+GGG + chr8_1:28240740-28240759 Msa1188160:intron 10.0%
!!! TTTAAATTCTTTGAAATTTT+TGG + chr8_1:28240741-28240760 Msa1188160:intron 10.0%
!!! TTTGTTTTTAAATTATTACT+AGG + chr8_1:28243152-28243171 None:intergenic 10.0%
!! TAATTCGAAATTAAAAACAT+TGG + chr8_1:28240348-28240367 Msa1188160:three_prime_UTR 15.0%
!! ATATATAAGTATGTATAAGT+GGG + chr8_1:28241432-28241451 None:intergenic 15.0%
!! TATATATAAGTATGTATAAG+TGG + chr8_1:28241433-28241452 None:intergenic 15.0%
!! TAAAATTAAATGTACATTTG+AGG + chr8_1:28242074-28242093 None:intergenic 15.0%
!!! ACATTTAATTTTAACTCAAA+TGG - chr8_1:28242081-28242100 Msa1188170:intron 15.0%
!!! TATTTAATAAAATGGATTAG+CGG + chr8_1:28242856-28242875 None:intergenic 15.0%
!! TTTGCACTTATTTAATAAAA+TGG + chr8_1:28242864-28242883 None:intergenic 15.0%
!! GAAAATTGATTATTGTTAAT+TGG + chr8_1:28243082-28243101 None:intergenic 15.0%
!! AAATGTGTATTTATAAACCA+AGG - chr8_1:28239993-28240012 Msa1188170:three_prime_UTR 20.0%
!!! CTAATTTTTGTACATAATGT+TGG - chr8_1:28240033-28240052 Msa1188170:three_prime_UTR 20.0%
!! ATAATAATAACTACACCTTT+GGG + chr8_1:28240205-28240224 Msa1188160:three_prime_UTR 20.0%
!! AATAATAATAACTACACCTT+TGG + chr8_1:28240206-28240225 Msa1188160:three_prime_UTR 20.0%
!!! GTAATCTTTAAAAAATCAAG+CGG + chr8_1:28240512-28240531 Msa1188160:three_prime_UTR 20.0%
!!! GTATGAATCAAATATTTTCA+TGG + chr8_1:28240638-28240657 Msa1188160:three_prime_UTR 20.0%
!! CAAATCTAAATTTGTCAATA+AGG - chr8_1:28240929-28240948 Msa1188170:intron 20.0%
!!! ATTTTTTCAACCAATAATTG+TGG + chr8_1:28241032-28241051 Msa1188160:intron 20.0%
!!! ATATTTTTGTGGACAAATTT+TGG - chr8_1:28241379-28241398 Msa1188170:intron 20.0%
!!! AAATTTTATTTTGCCTAAGA+TGG + chr8_1:28241597-28241616 None:intergenic 20.0%
!! TGAAAATGTAAGTAAAACAA+TGG - chr8_1:28241695-28241714 Msa1188170:intron 20.0%
!! TTTATTGGGTGAAATTAATA+TGG - chr8_1:28241936-28241955 Msa1188170:intron 20.0%
!! TTATTGGGTGAAATTAATAT+GGG - chr8_1:28241937-28241956 Msa1188170:intron 20.0%
!!! ATCAAACTTTATTTAACTCT+CGG - chr8_1:28242175-28242194 Msa1188170:intron 20.0%
!! ACTTTCAAACAATTATAACA+TGG + chr8_1:28242235-28242254 None:intergenic 20.0%
!! ACATGATTTCTTAATTATGT+AGG + chr8_1:28242399-28242418 None:intergenic 20.0%
!! TTGTTACATCTATATATGTT+TGG - chr8_1:28242633-28242652 Msa1188170:intron 20.0%
!! ATTTATTTGCATAGATTAGA+TGG + chr8_1:28242743-28242762 None:intergenic 20.0%
!!! TTTCTTTTTACAATCGTATT+TGG + chr8_1:28242805-28242824 None:intergenic 20.0%
!! TTGTATATGTCAATATGAAA+AGG + chr8_1:28243108-28243127 None:intergenic 20.0%
!!! GTTTTTAAATTATTACTAGG+TGG + chr8_1:28243149-28243168 None:intergenic 20.0%
!! ATCACTATAATCATTCTATA+TGG - chr8_1:28243521-28243540 Msa1188170:CDS 20.0%
!!! ATTTTGCTCATTAATATTAG+AGG + chr8_1:28243615-28243634 None:intergenic 20.0%
! TAATAATAACTACACCTTTG+GGG + chr8_1:28240204-28240223 Msa1188160:three_prime_UTR 25.0%
! ATGTCAATTCTATGTTCAAA+CGG - chr8_1:28240471-28240490 Msa1188170:intron 25.0%
! CTTGAAAATTTAGTCCTTAA+AGG - chr8_1:28240608-28240627 Msa1188170:CDS 25.0%
!!! TGAAAATTTTGTCGTTTAGA+TGG + chr8_1:28240705-28240724 Msa1188160:intron 25.0%
!! ATCTAAACGACAAAATTTTC+AGG - chr8_1:28240704-28240723 Msa1188170:intron 25.0%
! TAAGTATAGCTTAGAATAGT+TGG + chr8_1:28240966-28240985 Msa1188160:three_prime_UTR 25.0%
! GATATAATGTATTTGTCAAG+TGG - chr8_1:28241159-28241178 Msa1188170:intron 25.0%
!!! CTATTTTGAATTAGTTGTGA+AGG - chr8_1:28241352-28241371 Msa1188170:intron 25.0%
! AAATGTAAATGAGAAATGCT+AGG - chr8_1:28241875-28241894 Msa1188170:CDS 25.0%
! TATTACCATAGGAATATAGT+TGG - chr8_1:28242462-28242481 Msa1188170:intron 25.0%
! ATTTAATTCTCTTCTACATG+AGG + chr8_1:28242538-28242557 None:intergenic 25.0%
!! GTGCAAAACTAATTTTATCT+TGG - chr8_1:28242877-28242896 Msa1188170:CDS 25.0%
! TTTAAATTATTACTAGGTGG+TGG + chr8_1:28243146-28243165 None:intergenic 25.0%
! AAAGATTAAAAATCGCAACA+AGG - chr8_1:28243383-28243402 Msa1188170:intron 25.0%
! TGATTATAGTGATTGAAAGA+AGG + chr8_1:28243514-28243533 None:intergenic 25.0%
CTAATTAATCAACAACACCT+TGG + chr8_1:28240013-28240032 Msa1188160:three_prime_UTR 30.0%
! ATTGCACCATTTAGAATTTC+AGG + chr8_1:28240130-28240149 Msa1188160:three_prime_UTR 30.0%
AGTAAATATAAGCAACCGTT+AGG + chr8_1:28240171-28240190 Msa1188160:three_prime_UTR 30.0%
! CAGTTTTACCTTGATTCAAA+CGG + chr8_1:28240417-28240436 Msa1188160:three_prime_UTR 30.0%
! ATTACATTCGACTTAACTTG+TGG - chr8_1:28240527-28240546 Msa1188170:intron 30.0%
!! TTACATTCGACTTAACTTGT+GGG - chr8_1:28240528-28240547 Msa1188170:intron 30.0%
! ATTTTCATGGTCAACCTTTA+AGG + chr8_1:28240625-28240644 Msa1188160:three_prime_UTR 30.0%
AATTTGAATCTTGCAGCATT+GGG + chr8_1:28240788-28240807 Msa1188160:intron 30.0%
AAATTTGAATCTTGCAGCAT+TGG + chr8_1:28240789-28240808 Msa1188160:intron 30.0%
ATACAACTAGAGCAATACAT+GGG + chr8_1:28240886-28240905 Msa1188160:intron 30.0%
TTTCAACCAATAATTGTGGT+GGG + chr8_1:28241028-28241047 Msa1188160:intron 30.0%
! TTTTCAACCAATAATTGTGG+TGG + chr8_1:28241029-28241048 Msa1188160:intron 30.0%
GTCATTCAAATAGACCTTAT+GGG + chr8_1:28241066-28241085 Msa1188160:intron 30.0%
TGTCATTCAAATAGACCTTA+TGG + chr8_1:28241067-28241086 Msa1188160:intron 30.0%
TTTGTATAAATGTTGAGCAG+AGG - chr8_1:28241263-28241282 Msa1188170:intron 30.0%
ATCATATACTCCAATCACAT+TGG - chr8_1:28241316-28241335 Msa1188170:intron 30.0%
ATATCTGAATCCAATGTGAT+TGG + chr8_1:28241329-28241348 Msa1188160:three_prime_UTR 30.0%
ATCACATTGGATTCAGATAT+TGG - chr8_1:28241329-28241348 Msa1188170:intron 30.0%
!! GTGAAGGTACAATATTTTTG+TGG - chr8_1:28241368-28241387 Msa1188170:intron 30.0%
! TCACCATTTTAGACAAATGA+AGG - chr8_1:28241529-28241548 Msa1188170:intron 30.0%
CTTCCTTCATTTGTCTAAAA+TGG + chr8_1:28241535-28241554 None:intergenic 30.0%
! CATTTTAGACAAATGAAGGA+AGG - chr8_1:28241533-28241552 Msa1188170:intron 30.0%
! GTTTAGAGCTTCAATTTCTT+GGG + chr8_1:28241640-28241659 None:intergenic 30.0%
! TGTTTAGAGCTTCAATTTCT+TGG + chr8_1:28241641-28241660 None:intergenic 30.0%
!! TAAGTAAAACAATGGATTGC+TGG - chr8_1:28241703-28241722 Msa1188170:intron 30.0%
TCATTTGTACCTGAAACATT+GGG + chr8_1:28241806-28241825 None:intergenic 30.0%
TTCATTTGTACCTGAAACAT+TGG + chr8_1:28241807-28241826 None:intergenic 30.0%
! TACAAATGAATCTGCCATTT+AGG - chr8_1:28241817-28241836 Msa1188170:intron 30.0%
! TCTAGCACTCACTATTTTAT+TGG - chr8_1:28241921-28241940 Msa1188170:intron 30.0%
! CTAGCACTCACTATTTTATT+GGG - chr8_1:28241922-28241941 Msa1188170:intron 30.0%
CTGCAACAAAAGATAATAAC+AGG + chr8_1:28242282-28242301 None:intergenic 30.0%
TTATGTAGGTTACCTTTAGT+AGG + chr8_1:28242385-28242404 None:intergenic 30.0%
TATAGAACTGCTATTACCAT+AGG - chr8_1:28242451-28242470 Msa1188170:intron 30.0%
TCTTACCAACTATATTCCTA+TGG + chr8_1:28242470-28242489 None:intergenic 30.0%
TATTAAGAACGTGAAAGACA+TGG + chr8_1:28242708-28242727 None:intergenic 30.0%
TGTCTTTCACGTTCTTAATA+AGG - chr8_1:28242708-28242727 Msa1188170:intron 30.0%
! CTTTTTACAATCGTATTTGG+AGG + chr8_1:28242802-28242821 None:intergenic 30.0%
TGTTAATTGGCAATTAAACC+TGG + chr8_1:28243069-28243088 None:intergenic 30.0%
! ATTTTGCAGATACTTAGATG+TGG - chr8_1:28243181-28243200 Msa1188170:intron 30.0%
AAGCTAAGTGTCATGTTGAA+TGG + chr8_1:28240064-28240083 Msa1188160:three_prime_UTR 35.0%
GTAAGGACTTGAAAAATGCA+GGG + chr8_1:28240087-28240106 Msa1188160:three_prime_UTR 35.0%
CAAATTCTGAGCAAAAGGTA+AGG + chr8_1:28240104-28240123 Msa1188160:three_prime_UTR 35.0%
! CTTACCTTTTGCTCAGAATT+TGG - chr8_1:28240102-28240121 Msa1188170:three_prime_UTR 35.0%
TTGGAGCCTGAAATTCTAAA+TGG - chr8_1:28240121-28240140 Msa1188170:three_prime_UTR 35.0%
! AGAGCACTTATAAGATGAGA+TGG - chr8_1:28240306-28240325 Msa1188170:three_prime_UTR 35.0%
AACATATGAACCATGATACG+TGG + chr8_1:28240389-28240408 Msa1188160:three_prime_UTR 35.0%
! GTTTTACACCGTTTGAATCA+AGG - chr8_1:28240406-28240425 Msa1188170:CDS 35.0%
AAAAATCAAGCGGAACTAGA+TGG + chr8_1:28240502-28240521 Msa1188160:three_prime_UTR 35.0%
!!! GACAAAATTTTCAGGTAGCT+TGG - chr8_1:28240712-28240731 Msa1188170:intron 35.0%
ATTTGAATCTTGCAGCATTG+GGG + chr8_1:28240787-28240806 Msa1188160:intron 35.0%
TATCGAATTAGGAGTAGGAA+GGG + chr8_1:28240813-28240832 Msa1188160:intron 35.0%
ATATCGAATTAGGAGTAGGA+AGG + chr8_1:28240814-28240833 Msa1188160:intron 35.0%
TCCTACTCCTAATTCGATAT+TGG - chr8_1:28240814-28240833 Msa1188170:intron 35.0%
CCTACTCCTAATTCGATATT+GGG - chr8_1:28240815-28240834 Msa1188170:intron 35.0%
!!! GCAAGTATGTTTTTTCCAAC+AGG + chr8_1:28240845-28240864 Msa1188160:intron 35.0%
TTGGAAAAAACATACTTGCC+AGG - chr8_1:28240846-28240865 Msa1188170:intron 35.0%
TACAACTAGAGCAATACATG+GGG + chr8_1:28240885-28240904 Msa1188160:intron 35.0%
GATACAACTAGAGCAATACA+TGG + chr8_1:28240887-28240906 Msa1188160:intron 35.0%
AATTGCACTCAAATCCCATA+AGG - chr8_1:28241049-28241068 Msa1188170:intron 35.0%
! ACATCTCTCTACTTCTAGAA+AGG - chr8_1:28241086-28241105 Msa1188170:intron 35.0%
! TCTACTTCTAGAAAGGATCT+TGG - chr8_1:28241093-28241112 Msa1188170:intron 35.0%
! GGTGAGACATTTTTCCATTT+GGG + chr8_1:28241514-28241533 None:intergenic 35.0%
! TGGTGAGACATTTTTCCATT+TGG + chr8_1:28241515-28241534 None:intergenic 35.0%
!! AGATGGTAGAAAGTACCTTA+GGG + chr8_1:28241580-28241599 None:intergenic 35.0%
!! AAGATGGTAGAAAGTACCTT+AGG + chr8_1:28241581-28241600 None:intergenic 35.0%
! AAGGTACTTTCTACCATCTT+AGG - chr8_1:28241581-28241600 Msa1188170:intron 35.0%
TTACAAACCTGGTCAAGTTA+GGG - chr8_1:28241746-28241765 Msa1188170:intron 35.0%
CATTTGTACCTGAAACATTG+GGG + chr8_1:28241805-28241824 None:intergenic 35.0%
TATGTCCCACTACTTTATGT+GGG + chr8_1:28241984-28242003 None:intergenic 35.0%
! CTAGCACTCACTCTTTTATT+GGG + chr8_1:28242018-28242037 None:intergenic 35.0%
! TCTAGCACTCACTCTTTTAT+TGG + chr8_1:28242019-28242038 None:intergenic 35.0%
AGAATCGTTCAAGAAAACCT+AGG - chr8_1:28242125-28242144 Msa1188170:CDS 35.0%
GAAAACCTAGGTTTGATTCT+TGG - chr8_1:28242137-28242156 Msa1188170:CDS 35.0%
TTCATCCAAGAATCAAACCT+AGG + chr8_1:28242145-28242164 None:intergenic 35.0%
AAACCCAAAGGACAAAACAA+AGG - chr8_1:28242322-28242341 Msa1188170:CDS 35.0%
!!! ACACCTTTGTTTTGTCCTTT+GGG + chr8_1:28242328-28242347 None:intergenic 35.0%
!!! TACACCTTTGTTTTGTCCTT+TGG + chr8_1:28242329-28242348 None:intergenic 35.0%
!! AGCAAGAAAGTACTACTCTA+AGG + chr8_1:28242422-28242441 None:intergenic 35.0%
!! CTATATATGTTTGGTGATGC+AGG - chr8_1:28242642-28242661 Msa1188170:intron 35.0%
! TATCTTGGACACACATTTTC+TGG - chr8_1:28242892-28242911 Msa1188170:CDS 35.0%
! ATCTTGGACACACATTTTCT+GGG - chr8_1:28242893-28242912 Msa1188170:CDS 35.0%
TTTGACAGCCTCCTAATAAA+TGG - chr8_1:28242975-28242994 Msa1188170:intron 35.0%
ACACCACCATTCCATTTATT+AGG + chr8_1:28242989-28243008 None:intergenic 35.0%
! AATAAATGGAATGGTGGTGT+TGG - chr8_1:28242989-28243008 Msa1188170:intron 35.0%
! ATAAATGGAATGGTGGTGTT+GGG - chr8_1:28242990-28243009 Msa1188170:intron 35.0%
CAATTAAACCTGGATATCAG+GGG + chr8_1:28243059-28243078 None:intergenic 35.0%
GCAATTAAACCTGGATATCA+GGG + chr8_1:28243060-28243079 None:intergenic 35.0%
!! GTTGCTATGGCTTTATTCAT+GGG + chr8_1:28243214-28243233 None:intergenic 35.0%
!! AGTTGCTATGGCTTTATTCA+TGG + chr8_1:28243215-28243234 None:intergenic 35.0%
ACAATCATTGATCTGAGTGA+AGG - chr8_1:28243406-28243425 Msa1188170:intron 35.0%
TTGCTTCCATTTGTCAACAA+AGG - chr8_1:28243452-28243471 Msa1188170:intron 35.0%
TGCTTCCATTTGTCAACAAA+GGG - chr8_1:28243453-28243472 Msa1188170:intron 35.0%
! AAATTTTCAGTGCCAAGAAC+TGG + chr8_1:28243661-28243680 None:intergenic 35.0%
GGTAAGGACTTGAAAAATGC+AGG + chr8_1:28240088-28240107 Msa1188160:three_prime_UTR 40.0%
TACTATCGCAAAAACCCCAA+AGG - chr8_1:28240187-28240206 Msa1188170:three_prime_UTR 40.0%
ATCATCAAGACCACGTATCA+TGG - chr8_1:28240376-28240395 Msa1188170:exon 40.0%
CCCAATATCGAATTAGGAGT+AGG + chr8_1:28240818-28240837 Msa1188160:intron 40.0%
GGAAGTCCCAATATCGAATT+AGG + chr8_1:28240824-28240843 Msa1188160:intron 40.0%
!!! TGGGGAATCTTTTTCAAACC+TGG + chr8_1:28240867-28240886 Msa1188160:intron 40.0%
!! AACCAATAATTGTGGTGGGT+AGG + chr8_1:28241024-28241043 Msa1188160:intron 40.0%
! ACTTCTAGAAAGGATCTTGG+AGG - chr8_1:28241096-28241115 Msa1188170:intron 40.0%
GTTACAAACCTGGTCAAGTT+AGG - chr8_1:28241745-28241764 Msa1188170:intron 40.0%
! TGCCATTTAGGCACTTGAAA+AGG - chr8_1:28241829-28241848 Msa1188170:intron 40.0%
!!! CACCTTTTCAAGTGCCTAAA+TGG + chr8_1:28241834-28241853 None:intergenic 40.0%
TTAATATGGGACCTGCACTT+TGG - chr8_1:28241950-28241969 Msa1188170:intron 40.0%
ATCGAACCCACATAAAGTAG+TGG - chr8_1:28241975-28241994 Msa1188170:intron 40.0%
TCGAACCCACATAAAGTAGT+GGG - chr8_1:28241976-28241995 Msa1188170:intron 40.0%
GTATGTCCCACTACTTTATG+TGG + chr8_1:28241985-28242004 None:intergenic 40.0%
AGCCATACTAGAAAACCCAA+AGG - chr8_1:28242310-28242329 Msa1188170:CDS 40.0%
!! GTCCTTTGGGTTTTCTAGTA+TGG + chr8_1:28242315-28242334 None:intergenic 40.0%
CTGTCACATTGGCGTAAATA+AGG + chr8_1:28242352-28242371 None:intergenic 40.0%
TATTTACGCCAATGTGACAG+TGG - chr8_1:28242352-28242371 Msa1188170:CDS 40.0%
AGTGGACGATATCCTACTAA+AGG - chr8_1:28242370-28242389 Msa1188170:CDS 40.0%
GTAGTACTTTCTTGCTTGTG+AGG - chr8_1:28242426-28242445 Msa1188170:intron 40.0%
TGAAAGACATGGAATCTGTG+AGG + chr8_1:28242697-28242716 None:intergenic 40.0%
CAGCCTCCTAATAAATGGAA+TGG - chr8_1:28242980-28242999 Msa1188170:intron 40.0%
CCACCATTCCATTTATTAGG+AGG + chr8_1:28242986-28243005 None:intergenic 40.0%
CCTCCTAATAAATGGAATGG+TGG - chr8_1:28242983-28243002 Msa1188170:intron 40.0%
! TGGTGTTGGGTACATATCAA+AGG - chr8_1:28243003-28243022 Msa1188170:intron 40.0%
GGCAATTAAACCTGGATATC+AGG + chr8_1:28243061-28243080 None:intergenic 40.0%
!! TAGGTGGTGGTGATTTTTCT+TGG + chr8_1:28243133-28243152 None:intergenic 40.0%
GCTTCCATTTGTCAACAAAG+GGG - chr8_1:28243454-28243473 Msa1188170:intron 40.0%
TTTGCCCCTTTGTTGACAAA+TGG + chr8_1:28243461-28243480 None:intergenic 40.0%
ATACACAAACTTGCCAAGTC+AGG - chr8_1:28243635-28243654 Msa1188170:five_prime_UTR 40.0%
GGCTCCAAATTCTGAGCAAA+AGG + chr8_1:28240109-28240128 Msa1188160:three_prime_UTR 45.0%
GCAATTGCTCTTGTTGCTGT+TGG - chr8_1:28240145-28240164 Msa1188170:three_prime_UTR 45.0%
! TCTTGTTGCTGTTGGCCTAA+CGG - chr8_1:28240153-28240172 Msa1188170:three_prime_UTR 45.0%
TCGATATTGGGACTTCCTGT+TGG - chr8_1:28240827-28240846 Msa1188170:intron 45.0%
CACCTACCCACCACAATTAT+TGG - chr8_1:28241019-28241038 Msa1188170:intron 45.0%
AGCAGAGGAAGAGATAGTCA+AGG - chr8_1:28241278-28241297 Msa1188170:intron 45.0%
TGCAAGCAGATGTACCCAAA+TGG - chr8_1:28241497-28241516 Msa1188170:intron 45.0%
AATGAAGGAAGGTGACACCT+TGG - chr8_1:28241544-28241563 Msa1188170:intron 45.0%
!! AATGGATTGCTGGAGCTTGT+AGG - chr8_1:28241713-28241732 Msa1188170:intron 45.0%
! ATGGATTGCTGGAGCTTGTA+GGG - chr8_1:28241714-28241733 Msa1188170:intron 45.0%
GGACGTTTCAGTTACAAACC+TGG - chr8_1:28241735-28241754 Msa1188170:intron 45.0%
! AGGGCATTTGGAATGATTGC+TGG - chr8_1:28241765-28241784 Msa1188170:intron 45.0%
! GCATTTGGAATGATTGCTGG+AGG - chr8_1:28241768-28241787 Msa1188170:intron 45.0%
AGGCATTACCCCAATGTTTC+AGG - chr8_1:28241794-28241813 Msa1188170:intron 45.0%
GATATCGTCCACTGTCACAT+TGG + chr8_1:28242363-28242382 None:intergenic 45.0%
!! ATGGCTTTATTCATGGGTGG+TGG + chr8_1:28243208-28243227 None:intergenic 45.0%
!! GCTATGGCTTTATTCATGGG+TGG + chr8_1:28243211-28243230 None:intergenic 45.0%
TGCTGCAAGATGAGTTGCTA+TGG + chr8_1:28243227-28243246 None:intergenic 45.0%
!!! TTTTTCAAATATATTTATTA+AGG + chr8_1:28241214-28241233 Msa1188160:intron 5.0%
!! ATAAATATATTTGAAAAAAA+AGG - chr8_1:28241216-28241235 Msa1188170:intron 5.0%
!! AAATAATTATAATATGAATT+CGG + chr8_1:28242201-28242220 None:intergenic 5.0%
!! TAATAATTTAAAAACAAAAA+TGG - chr8_1:28243154-28243173 Msa1188170:intron 5.0%
AAGTACCTTAGGGCCCTTCA+CGG + chr8_1:28241570-28241589 None:intergenic 50.0%
CCAAATGCCCTAACTTGACC+AGG + chr8_1:28241756-28241775 None:intergenic 50.0%
! CCTGGTCAAGTTAGGGCATT+TGG - chr8_1:28241753-28241772 Msa1188170:intron 50.0%
GGAATGATTGCTGGAGGTTC+AGG - chr8_1:28241774-28241793 Msa1188170:intron 50.0%
GGGTTCGATTTCCAAAGTGC+AGG + chr8_1:28241964-28241983 None:intergenic 50.0%
TAAACCTGGATATCAGGGGC+AGG + chr8_1:28243055-28243074 None:intergenic 50.0%
GCAACTCATCTTGCAGCACT+TGG - chr8_1:28243229-28243248 Msa1188170:intron 50.0%
! TGCAGCATTGGGGCTGATCA+TGG + chr8_1:28240777-28240796 Msa1188160:intron 55.0%
TGCCAAGTCAGGCCAGTTCT+TGG - chr8_1:28243646-28243665 Msa1188170:five_prime_UTR 55.0%
! TGCCAAGAACTGGCCTGACT+TGG + chr8_1:28243651-28243670 None:intergenic 55.0%
AGGTGACACCTTGGCCGTGA+AGG - chr8_1:28241553-28241572 Msa1188170:intron 60.0%
GGTGACACCTTGGCCGTGAA+GGG - chr8_1:28241554-28241573 Msa1188170:intron 60.0%
TGGATATCAGGGGCAGGTGC+TGG + chr8_1:28243049-28243068 None:intergenic 60.0%
AGCACCTGCCCCTGATATCC+AGG - chr8_1:28243048-28243067 Msa1188170:intron 60.0%
CTTAGGGCCCTTCACGGCCA+AGG + chr8_1:28241564-28241583 None:intergenic 65.0%
CTTGGCCGTGAAGGGCCCTA+AGG - chr8_1:28241562-28241581 Msa1188170:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 28239991 28243681 28239991 ID=Msa1188170;Name=Msa1188170
chr8_1 mRNA 28239991 28243681 28239991 ID=Msa1188170-mRNA-1;Parent=Msa1188170;Name=Msa1188170-mRNA-1;_AED=0.15;_eAED=0.15;_QI=58|0.85|1|1|1|1|8|399|265
chr8_1 exon 28239991 28240482 28239991 ID=Msa1188170-mRNA-1:exon:9956;Parent=Msa1188170-mRNA-1
chr8_1 exon 28240603 28240689 28240603 ID=Msa1188170-mRNA-1:exon:9955;Parent=Msa1188170-mRNA-1
chr8_1 exon 28240943 28241008 28240943 ID=Msa1188170-mRNA-1:exon:9954;Parent=Msa1188170-mRNA-1
chr8_1 exon 28241857 28241937 28241857 ID=Msa1188170-mRNA-1:exon:9953;Parent=Msa1188170-mRNA-1
chr8_1 exon 28242089 28242166 28242089 ID=Msa1188170-mRNA-1:exon:9952;Parent=Msa1188170-mRNA-1
chr8_1 exon 28242299 28242389 28242299 ID=Msa1188170-mRNA-1:exon:9951;Parent=Msa1188170-mRNA-1
chr8_1 exon 28242805 28242946 28242805 ID=Msa1188170-mRNA-1:exon:9950;Parent=Msa1188170-mRNA-1
chr8_1 exon 28243464 28243681 28243464 ID=Msa1188170-mRNA-1:exon:9949;Parent=Msa1188170-mRNA-1
chr8_1 five_prime_UTR 28243624 28243681 28243624 ID=Msa1188170-mRNA-1:five_prime_utr;Parent=Msa1188170-mRNA-1
chr8_1 CDS 28243464 28243623 28243464 ID=Msa1188170-mRNA-1:cds;Parent=Msa1188170-mRNA-1
chr8_1 CDS 28242805 28242946 28242805 ID=Msa1188170-mRNA-1:cds;Parent=Msa1188170-mRNA-1
chr8_1 CDS 28242299 28242389 28242299 ID=Msa1188170-mRNA-1:cds;Parent=Msa1188170-mRNA-1
chr8_1 CDS 28242089 28242166 28242089 ID=Msa1188170-mRNA-1:cds;Parent=Msa1188170-mRNA-1
chr8_1 CDS 28241857 28241937 28241857 ID=Msa1188170-mRNA-1:cds;Parent=Msa1188170-mRNA-1
chr8_1 CDS 28240943 28241008 28240943 ID=Msa1188170-mRNA-1:cds;Parent=Msa1188170-mRNA-1
chr8_1 CDS 28240603 28240689 28240603 ID=Msa1188170-mRNA-1:cds;Parent=Msa1188170-mRNA-1
chr8_1 CDS 28240390 28240482 28240390 ID=Msa1188170-mRNA-1:cds;Parent=Msa1188170-mRNA-1
chr8_1 three_prime_UTR 28239991 28240389 28239991 ID=Msa1188170-mRNA-1:three_prime_utr;Parent=Msa1188170-mRNA-1
Gene Sequence

>Msa1188170

ATGTTGGAGTTACCATTCAACATGACACTTAGCTTCCCTGCATTTTTCAAGTCCTTACCTTTTGCTCAGAATTTGGAGCCTGAAATTCTAAATGGTGCAATTGCTCTTGTTGCTGTTGGCCTAACGGTTGCTTATATTTACTATCGCAAAAACCCCAAAGGTAGCTTGGATCCCAAAAATTTCAAAGAATTTAAACTTGTAAAGAAGACCATGATCAGCCCCAATGCTGCAAGATTCAAATTTGCCCTTCCTACTCCTAATTCGATATTGGGACTTCCTGTTGGAAAAAACATACTTGCCAGAGGAAGAGATAGTCAAGGAGAAGAAGTTATGAGATCATATACTCCAATCACATTGGATTCAGATATTGGCTATTTTGAATTAGTTGTGAAGATGTACCCAAATGGAAAAATGTCTCACCATTTTAGACAAATGAAGGAAGGTGACACCTTGGCCGTGAAGGGCCCTAAGGGACGTTTCAGTTACAAACCTGGTCAAGTTAGGGCATTTGGAATGATTGCTGGAGGTTCAGGCATTACCCCAATGTTTCAGGAAGAACTAGAATTCTTTGCAGACAAATTTCCTCACAGATTCCATGTCTTTCACGTTCTTAATAAGCCTCCTAATAAATGGAATGGTGGTGTTGGGTACATATCAAAGGAGATGATTGAATCTCATTGTCCAGCACCTGCCCCTGATATCCAGATACTTAGATGTGGTCCACCACCCATGAATAAAGCCATAGCAACTCATCTTGCAGCACTTGGATACACATCAAACATGCAGTTCGAGTTCTAA
Protein sequence

>Msa1188170

MLELPFNMTLSFPAFFKSLPFAQNLEPEILNGAIALVAVGLTVAYIYYRKNPKGSLDPKNFKEFKLVKKTMISPNAARFKFALPTPNSILGLPVGKNILARGRDSQGEEVMRSYTPITLDSDIGYFELVVKMYPNGKMSHHFRQMKEGDTLAVKGPKGRFSYKPGQVRAFGMIAGGSGITPMFQEELEFFADKFPHRFHVFHVLNKPPNKWNGGVGYISKEMIESHCPAPAPDIQILRCGPPPMNKAIATHLAALGYTSNMQFEF*