AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1353680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1353680 MtrunA17_Chr5g0400001 100.000 150 0 0 1 150 100 249 3.16e-108 318
Msa1353680 MtrunA17_Chr5g0399991 100.000 150 0 0 1 150 102 251 6.13e-108 318
Msa1353680 MtrunA17_Chr4g0055281 88.591 149 17 0 1 149 91 239 1.81e-96 288
Msa1353680 MtrunA17_Chr6g0481031 62.667 150 56 0 1 150 119 268 3.48e-69 219
Msa1353680 MtrunA17_Chr8g0388651 55.102 147 66 0 3 149 94 240 9.38e-53 175
Msa1353680 MtrunA17_Chr2g0323051 55.319 141 63 0 11 151 109 249 2.19e-52 175
Msa1353680 MtrunA17_Chr4g0023601 50.694 144 71 0 10 153 35 178 5.02e-52 172
Msa1353680 MtrunA17_Chr7g0247631 55.882 136 60 0 15 150 292 427 5.18e-47 162
Msa1353680 MtrunA17_Chr5g0408851 42.177 147 85 0 4 150 234 380 5.63e-36 131
Msa1353680 MtrunA17_Chr2g0286431 39.726 146 83 1 5 150 101 241 1.62e-33 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1353680 AT4G17090.1 87.248 149 19 0 1 149 99 247 3.51e-94 283
Msa1353680 AT3G23920.1 64.000 150 54 0 1 150 119 268 6.26e-68 216
Msa1353680 AT4G15210.2 48.276 145 74 1 9 152 35 179 1.90e-47 159
Msa1353680 AT4G15210.1 48.276 145 74 1 9 152 35 179 4.95e-47 159
Msa1353680 AT2G45880.1 54.412 136 62 0 15 150 275 410 8.07e-46 159
Msa1353680 AT4G00490.1 49.333 150 76 0 1 150 108 257 1.04e-45 157
Msa1353680 AT2G32290.1 51.163 129 63 0 23 151 113 241 8.85e-45 155
Msa1353680 AT4G15210.3 49.206 126 64 0 27 152 1 126 1.74e-43 149
Msa1353680 AT4G15210.4 49.206 126 64 0 27 152 1 126 1.74e-43 149
Msa1353680 AT5G55700.1 41.781 146 85 0 4 149 112 257 1.60e-36 132
Msa1353680 AT5G45300.3 42.000 150 87 0 3 152 284 433 2.72e-35 130
Msa1353680 AT5G45300.2 42.000 150 87 0 3 152 268 417 2.91e-35 129
Msa1353680 AT5G45300.1 42.000 150 87 0 3 152 270 419 3.20e-35 129
Msa1353680 AT5G18670.1 39.310 145 83 1 5 149 104 243 1.26e-30 116
Msa1353680 AT5G55700.2 48.214 56 29 0 94 149 39 94 1.35e-11 62.0

Find 60 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAACTTGGTCTTCAATTT+TGG 0.089455 tig0013921:+5906 Msa1353680:CDS
TGATAGATTCACAGATTATT+TGG 0.131732 tig0013921:+5860 Msa1353680:CDS
ATGGTCCTATGAAGTATAAT+TGG 0.246951 tig0013921:+5362 Msa1353680:CDS
CTGGTTTACACAGACAAATT+AGG 0.254676 tig0013921:+5735 Msa1353680:CDS
GGTTGATGCTTGGTGGGGTT+TGG 0.299766 tig0013921:+5330 Msa1353680:CDS
TGAAGCAAAGGTTTGAATTT+CGG 0.301327 tig0013921:-5053 None:intergenic
CATGGGTGCTTGAAGAAATA+AGG 0.350399 tig0013921:+5700 Msa1353680:CDS
GAAATGTTGGTGACTCTTGC+AGG 0.358515 tig0013921:+5467 Msa1353680:CDS
ACAGCCCAACGAGATGTATT+CGG 0.366361 tig0013921:-5769 None:intergenic
GATAGATTCACAGATTATTT+GGG 0.369481 tig0013921:+5861 Msa1353680:CDS
AGCAATGAATGCTAGTTTGA+TGG 0.376383 tig0013921:+5276 Msa1353680:CDS
TGGTTTACACAGACAAATTA+GGG 0.392347 tig0013921:+5736 Msa1353680:CDS
AAACACCAACTCTGCAGTTA+AGG 0.394173 tig0013921:+5121 Msa1353680:five_prime_UTR
CAGTTAAGGAAAACAAGAAA+TGG 0.395175 tig0013921:+5135 Msa1353680:five_prime_UTR
AGCTGGAAGAACTCCTCTTC+AGG 0.398700 tig0013921:+5809 Msa1353680:CDS
TCCCATTGTACCGTGTCTAA+TGG 0.401367 tig0013921:-5233 None:intergenic
TTGTCTGTGTAAACCAGCTC+AGG 0.410476 tig0013921:-5729 None:intergenic
TTATGCTGAGCTTGTTCAAA+TGG 0.410701 tig0013921:+5390 Msa1353680:CDS
GCTTCCCAATTATACTTCAT+AGG 0.415265 tig0013921:-5367 None:intergenic
CAGCCCAACGAGATGTATTC+GGG 0.415616 tig0013921:-5768 None:intergenic
CTAGCATACCTTTACCTCCA+TGG 0.426539 tig0013921:+5682 Msa1353680:intron
TTGAAGAGTGCTGGAGTTGA+AGG 0.429269 tig0013921:+5301 Msa1353680:CDS
ACGGAAGCTTCTCATGTAAT+CGG 0.432165 tig0013921:-5838 None:intergenic
GGAGTTCTTCCAGCTAGAAC+AGG 0.442830 tig0013921:-5801 None:intergenic
CATAGTAAGTTTCACCAACT+TGG 0.443306 tig0013921:+5893 Msa1353680:CDS
TAGCATACCTTTACCTCCAT+GGG 0.456896 tig0013921:+5683 Msa1353680:intron
GCCATTAGACACGGTACAAT+GGG 0.457333 tig0013921:+5232 Msa1353680:exon
GATTCAGTGCCTGTTCTAGC+TGG 0.467117 tig0013921:+5792 Msa1353680:CDS
TTGATGGCTTTGAAGAGTGC+TGG 0.467245 tig0013921:+5292 Msa1353680:CDS
TCAAGCACCCATGGAGGTAA+AGG 0.469083 tig0013921:-5690 None:intergenic
AGTAATGGTTGATGCTTGGT+GGG 0.476693 tig0013921:+5324 Msa1353680:CDS
TGGTCCTATGAAGTATAATT+GGG 0.480087 tig0013921:+5363 Msa1353680:CDS
CTTATTTCTTCAAGCACCCA+TGG 0.485962 tig0013921:-5699 None:intergenic
GAGTAATGGTTGATGCTTGG+TGG 0.507394 tig0013921:+5323 Msa1353680:CDS
AGTTAAGGAAAACAAGAAAT+GGG 0.509470 tig0013921:+5136 Msa1353680:five_prime_UTR
GTTCAAATGGTACAAAAGCA+TGG 0.513572 tig0013921:+5403 Msa1353680:CDS
CATCAATGTGGTGGAAATGT+TGG 0.516528 tig0013921:+5454 Msa1353680:CDS
GTAATGGTTGATGCTTGGTG+GGG 0.525670 tig0013921:+5325 Msa1353680:CDS
AATCCCGAATACATCTCGTT+GGG 0.532457 tig0013921:+5765 Msa1353680:CDS
TTTCGGACAAGAAACTTTGT+CGG 0.539192 tig0013921:-5036 None:intergenic
TGCTGGAGTTGAAGGAGTAA+TGG 0.546595 tig0013921:+5309 Msa1353680:CDS
CCCAAAATTGAAGACCAAGT+TGG 0.562842 tig0013921:-5907 None:intergenic
AAGGAGTAATGGTTGATGCT+TGG 0.565190 tig0013921:+5320 Msa1353680:CDS
GAATCCCGAATACATCTCGT+TGG 0.567655 tig0013921:+5764 Msa1353680:CDS
ACACAGACAAATTAGGGAGG+AGG 0.571216 tig0013921:+5742 Msa1353680:CDS
AATAAGGAAGAACCCTGAGC+TGG 0.576300 tig0013921:+5716 Msa1353680:CDS
TGCCATTAGACACGGTACAA+TGG 0.576857 tig0013921:+5231 Msa1353680:exon
TTTGCTTTGAGATGAAGCAA+AGG 0.577596 tig0013921:-5065 None:intergenic
TAATCGGAGTAGACCTGAAG+AGG 0.578784 tig0013921:-5822 None:intergenic
TGCTGACTCAAATAGAGTAC+CGG 0.579953 tig0013921:+5196 Msa1353680:five_prime_UTR
AATAATCTGTGAATCTATCA+CGG 0.584013 tig0013921:-5857 None:intergenic
TGTCTGTGTAAACCAGCTCA+GGG 0.638501 tig0013921:-5728 None:intergenic
ATTAGACACGGTACAATGGG+AGG 0.641924 tig0013921:+5235 Msa1353680:exon
TCGTGGTTATGAACAATCGA+TGG 0.648491 tig0013921:-5173 None:intergenic
AATGGCAACATCACAAACAC+CGG 0.660873 tig0013921:-5215 None:intergenic
GCAAGAAACACATTTCACAA+AGG 0.674864 tig0013921:+5097 Msa1353680:five_prime_UTR
ATTTCTTCAAGCACCCATGG+AGG 0.686996 tig0013921:-5696 None:intergenic
TGTGATGTTGCCATTAGACA+CGG 0.699044 tig0013921:+5223 Msa1353680:five_prime_UTR
CTCTATTTGAGTCAGCATCG+TGG 0.708875 tig0013921:-5190 None:intergenic
TTTACACAGACAAATTAGGG+AGG 0.734523 tig0013921:+5739 Msa1353680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTATCATATTTTTTATTTT+TGG - tig0013921:5640-5659 None:intergenic 10.0%
!!! AGTATCATATTTTTTATTTT+TGG - tig0013921:5640-5659 None:intergenic 10.0%
!!! TGTTTCTTTTTTATTAATGA+AGG - tig0013921:4984-5003 None:intergenic 15.0%
!!! TGTTTCTTTTTTATTAATGA+AGG - tig0013921:4984-5003 None:intergenic 15.0%
! AGTTAAGGAAAACAAGAAAT+GGG + tig0013921:5136-5155 Msa1353680:five_prime_UTR 25.0%
! TAATATTCAACCACCAATTT+AGG + tig0013921:5517-5536 Msa1353680:intron 25.0%
! AATAATCTGTGAATCTATCA+CGG - tig0013921:5860-5879 None:intergenic 25.0%
! TGATAGATTCACAGATTATT+TGG + tig0013921:5860-5879 Msa1353680:CDS 25.0%
! GATAGATTCACAGATTATTT+GGG + tig0013921:5861-5880 Msa1353680:CDS 25.0%
! AGTTAAGGAAAACAAGAAAT+GGG + tig0013921:5136-5155 Msa1353680:five_prime_UTR 25.0%
! TAATATTCAACCACCAATTT+AGG + tig0013921:5517-5536 Msa1353680:intron 25.0%
! AATAATCTGTGAATCTATCA+CGG - tig0013921:5860-5879 None:intergenic 25.0%
! TGATAGATTCACAGATTATT+TGG + tig0013921:5860-5879 Msa1353680:CDS 25.0%
! GATAGATTCACAGATTATTT+GGG + tig0013921:5861-5880 Msa1353680:CDS 25.0%
AGTAGAGCAAACACTAAAAA+TGG + tig0013921:4934-4953 Msa1353680:five_prime_UTR 30.0%
TGAAGCAAAGGTTTGAATTT+CGG - tig0013921:5056-5075 None:intergenic 30.0%
CAGTTAAGGAAAACAAGAAA+TGG + tig0013921:5135-5154 Msa1353680:five_prime_UTR 30.0%
ATGGTCCTATGAAGTATAAT+TGG + tig0013921:5362-5381 Msa1353680:CDS 30.0%
TGGTCCTATGAAGTATAATT+GGG + tig0013921:5363-5382 Msa1353680:CDS 30.0%
! GTTATGTCTTTTCATCAATG+TGG + tig0013921:5442-5461 Msa1353680:CDS 30.0%
ACACTAAATAGAGCCTAAAT+TGG - tig0013921:5533-5552 None:intergenic 30.0%
TGGTTTACACAGACAAATTA+GGG + tig0013921:5736-5755 Msa1353680:CDS 30.0%
AGTAGAGCAAACACTAAAAA+TGG + tig0013921:4934-4953 Msa1353680:five_prime_UTR 30.0%
TGAAGCAAAGGTTTGAATTT+CGG - tig0013921:5056-5075 None:intergenic 30.0%
CAGTTAAGGAAAACAAGAAA+TGG + tig0013921:5135-5154 Msa1353680:five_prime_UTR 30.0%
ATGGTCCTATGAAGTATAAT+TGG + tig0013921:5362-5381 Msa1353680:CDS 30.0%
TGGTCCTATGAAGTATAATT+GGG + tig0013921:5363-5382 Msa1353680:CDS 30.0%
! GTTATGTCTTTTCATCAATG+TGG + tig0013921:5442-5461 Msa1353680:CDS 30.0%
ACACTAAATAGAGCCTAAAT+TGG - tig0013921:5533-5552 None:intergenic 30.0%
TGGTTTACACAGACAAATTA+GGG + tig0013921:5736-5755 Msa1353680:CDS 30.0%
TTTCGGACAAGAAACTTTGT+CGG - tig0013921:5039-5058 None:intergenic 35.0%
!! TTTGCTTTGAGATGAAGCAA+AGG - tig0013921:5068-5087 None:intergenic 35.0%
GCAAGAAACACATTTCACAA+AGG + tig0013921:5097-5116 Msa1353680:five_prime_UTR 35.0%
! AGCAATGAATGCTAGTTTGA+TGG + tig0013921:5276-5295 Msa1353680:CDS 35.0%
GCTTCCCAATTATACTTCAT+AGG - tig0013921:5370-5389 None:intergenic 35.0%
! TTATGCTGAGCTTGTTCAAA+TGG + tig0013921:5390-5409 Msa1353680:CDS 35.0%
GTTCAAATGGTACAAAAGCA+TGG + tig0013921:5403-5422 Msa1353680:CDS 35.0%
! ATGTCTTTTCATCAATGTGG+TGG + tig0013921:5445-5464 Msa1353680:CDS 35.0%
CTAAATAGAGCCTAAATTGG+TGG - tig0013921:5530-5549 None:intergenic 35.0%
CTGGTTTACACAGACAAATT+AGG + tig0013921:5735-5754 Msa1353680:CDS 35.0%
TTTACACAGACAAATTAGGG+AGG + tig0013921:5739-5758 Msa1353680:CDS 35.0%
CATAGTAAGTTTCACCAACT+TGG + tig0013921:5893-5912 Msa1353680:CDS 35.0%
!! ACCAACTTGGTCTTCAATTT+TGG + tig0013921:5906-5925 Msa1353680:CDS 35.0%
!! CCAACTTGGTCTTCAATTTT+GGG + tig0013921:5907-5926 Msa1353680:CDS 35.0%
!! CAACTTGGTCTTCAATTTTG+GGG + tig0013921:5908-5927 Msa1353680:CDS 35.0%
!!! TCTTCAATTTTGGGGTTACT+TGG + tig0013921:5916-5935 Msa1353680:CDS 35.0%
TTTCGGACAAGAAACTTTGT+CGG - tig0013921:5039-5058 None:intergenic 35.0%
!! TTTGCTTTGAGATGAAGCAA+AGG - tig0013921:5068-5087 None:intergenic 35.0%
GCAAGAAACACATTTCACAA+AGG + tig0013921:5097-5116 Msa1353680:five_prime_UTR 35.0%
! AGCAATGAATGCTAGTTTGA+TGG + tig0013921:5276-5295 Msa1353680:CDS 35.0%
GCTTCCCAATTATACTTCAT+AGG - tig0013921:5370-5389 None:intergenic 35.0%
! TTATGCTGAGCTTGTTCAAA+TGG + tig0013921:5390-5409 Msa1353680:CDS 35.0%
GTTCAAATGGTACAAAAGCA+TGG + tig0013921:5403-5422 Msa1353680:CDS 35.0%
! ATGTCTTTTCATCAATGTGG+TGG + tig0013921:5445-5464 Msa1353680:CDS 35.0%
CTAAATAGAGCCTAAATTGG+TGG - tig0013921:5530-5549 None:intergenic 35.0%
CTGGTTTACACAGACAAATT+AGG + tig0013921:5735-5754 Msa1353680:CDS 35.0%
TTTACACAGACAAATTAGGG+AGG + tig0013921:5739-5758 Msa1353680:CDS 35.0%
CATAGTAAGTTTCACCAACT+TGG + tig0013921:5893-5912 Msa1353680:CDS 35.0%
!! ACCAACTTGGTCTTCAATTT+TGG + tig0013921:5906-5925 Msa1353680:CDS 35.0%
!! CCAACTTGGTCTTCAATTTT+GGG + tig0013921:5907-5926 Msa1353680:CDS 35.0%
!! CAACTTGGTCTTCAATTTTG+GGG + tig0013921:5908-5927 Msa1353680:CDS 35.0%
!!! TCTTCAATTTTGGGGTTACT+TGG + tig0013921:5916-5935 Msa1353680:CDS 35.0%
AAACACCAACTCTGCAGTTA+AGG + tig0013921:5121-5140 Msa1353680:five_prime_UTR 40.0%
! GTTTTCCTTAACTGCAGAGT+TGG - tig0013921:5129-5148 None:intergenic 40.0%
TCGTGGTTATGAACAATCGA+TGG - tig0013921:5176-5195 None:intergenic 40.0%
!! TGCTGACTCAAATAGAGTAC+CGG + tig0013921:5196-5215 Msa1353680:five_prime_UTR 40.0%
AATGGCAACATCACAAACAC+CGG - tig0013921:5218-5237 None:intergenic 40.0%
TGTGATGTTGCCATTAGACA+CGG + tig0013921:5223-5242 Msa1353680:five_prime_UTR 40.0%
! AAGGAGTAATGGTTGATGCT+TGG + tig0013921:5320-5339 Msa1353680:CDS 40.0%
! AGTAATGGTTGATGCTTGGT+GGG + tig0013921:5324-5343 Msa1353680:CDS 40.0%
! TGGGGTTTGGTTGAAAAAGA+TGG + tig0013921:5343-5362 Msa1353680:CDS 40.0%
! CATCAATGTGGTGGAAATGT+TGG + tig0013921:5454-5473 Msa1353680:CDS 40.0%
TAGCATACCTTTACCTCCAT+GGG + tig0013921:5683-5702 Msa1353680:intron 40.0%
! CTTATTTCTTCAAGCACCCA+TGG - tig0013921:5702-5721 None:intergenic 40.0%
CATGGGTGCTTGAAGAAATA+AGG + tig0013921:5700-5719 Msa1353680:CDS 40.0%
AATCCCGAATACATCTCGTT+GGG + tig0013921:5765-5784 Msa1353680:CDS 40.0%
ACGGAAGCTTCTCATGTAAT+CGG - tig0013921:5841-5860 None:intergenic 40.0%
CCCAAAATTGAAGACCAAGT+TGG - tig0013921:5910-5929 None:intergenic 40.0%
AAACACCAACTCTGCAGTTA+AGG + tig0013921:5121-5140 Msa1353680:five_prime_UTR 40.0%
! GTTTTCCTTAACTGCAGAGT+TGG - tig0013921:5129-5148 None:intergenic 40.0%
TCGTGGTTATGAACAATCGA+TGG - tig0013921:5176-5195 None:intergenic 40.0%
!! TGCTGACTCAAATAGAGTAC+CGG + tig0013921:5196-5215 Msa1353680:five_prime_UTR 40.0%
AATGGCAACATCACAAACAC+CGG - tig0013921:5218-5237 None:intergenic 40.0%
TGTGATGTTGCCATTAGACA+CGG + tig0013921:5223-5242 Msa1353680:five_prime_UTR 40.0%
! AAGGAGTAATGGTTGATGCT+TGG + tig0013921:5320-5339 Msa1353680:CDS 40.0%
! AGTAATGGTTGATGCTTGGT+GGG + tig0013921:5324-5343 Msa1353680:CDS 40.0%
! TGGGGTTTGGTTGAAAAAGA+TGG + tig0013921:5343-5362 Msa1353680:CDS 40.0%
! CATCAATGTGGTGGAAATGT+TGG + tig0013921:5454-5473 Msa1353680:CDS 40.0%
TAGCATACCTTTACCTCCAT+GGG + tig0013921:5683-5702 Msa1353680:intron 40.0%
! CTTATTTCTTCAAGCACCCA+TGG - tig0013921:5702-5721 None:intergenic 40.0%
CATGGGTGCTTGAAGAAATA+AGG + tig0013921:5700-5719 Msa1353680:CDS 40.0%
AATCCCGAATACATCTCGTT+GGG + tig0013921:5765-5784 Msa1353680:CDS 40.0%
ACGGAAGCTTCTCATGTAAT+CGG - tig0013921:5841-5860 None:intergenic 40.0%
CCCAAAATTGAAGACCAAGT+TGG - tig0013921:5910-5929 None:intergenic 40.0%
CTCTATTTGAGTCAGCATCG+TGG - tig0013921:5193-5212 None:intergenic 45.0%
TGCCATTAGACACGGTACAA+TGG + tig0013921:5231-5250 Msa1353680:exon 45.0%
GCCATTAGACACGGTACAAT+GGG + tig0013921:5232-5251 Msa1353680:exon 45.0%
TCCCATTGTACCGTGTCTAA+TGG - tig0013921:5236-5255 None:intergenic 45.0%
ATTAGACACGGTACAATGGG+AGG + tig0013921:5235-5254 Msa1353680:exon 45.0%
!! TTGATGGCTTTGAAGAGTGC+TGG + tig0013921:5292-5311 Msa1353680:CDS 45.0%
!! TTGAAGAGTGCTGGAGTTGA+AGG + tig0013921:5301-5320 Msa1353680:CDS 45.0%
TGCTGGAGTTGAAGGAGTAA+TGG + tig0013921:5309-5328 Msa1353680:CDS 45.0%
! GAGTAATGGTTGATGCTTGG+TGG + tig0013921:5323-5342 Msa1353680:CDS 45.0%
! GTAATGGTTGATGCTTGGTG+GGG + tig0013921:5325-5344 Msa1353680:CDS 45.0%
!! GAAATGTTGGTGACTCTTGC+AGG + tig0013921:5467-5486 Msa1353680:CDS 45.0%
CTAGCATACCTTTACCTCCA+TGG + tig0013921:5682-5701 Msa1353680:intron 45.0%
ATTTCTTCAAGCACCCATGG+AGG - tig0013921:5699-5718 None:intergenic 45.0%
AATAAGGAAGAACCCTGAGC+TGG + tig0013921:5716-5735 Msa1353680:CDS 45.0%
TGTCTGTGTAAACCAGCTCA+GGG - tig0013921:5731-5750 None:intergenic 45.0%
TTGTCTGTGTAAACCAGCTC+AGG - tig0013921:5732-5751 None:intergenic 45.0%
ACACAGACAAATTAGGGAGG+AGG + tig0013921:5742-5761 Msa1353680:CDS 45.0%
GAATCCCGAATACATCTCGT+TGG + tig0013921:5764-5783 Msa1353680:CDS 45.0%
ACAGCCCAACGAGATGTATT+CGG - tig0013921:5772-5791 None:intergenic 45.0%
TAATCGGAGTAGACCTGAAG+AGG - tig0013921:5825-5844 None:intergenic 45.0%
CTCTATTTGAGTCAGCATCG+TGG - tig0013921:5193-5212 None:intergenic 45.0%
TGCCATTAGACACGGTACAA+TGG + tig0013921:5231-5250 Msa1353680:exon 45.0%
GCCATTAGACACGGTACAAT+GGG + tig0013921:5232-5251 Msa1353680:exon 45.0%
TCCCATTGTACCGTGTCTAA+TGG - tig0013921:5236-5255 None:intergenic 45.0%
ATTAGACACGGTACAATGGG+AGG + tig0013921:5235-5254 Msa1353680:exon 45.0%
!! TTGATGGCTTTGAAGAGTGC+TGG + tig0013921:5292-5311 Msa1353680:CDS 45.0%
!! TTGAAGAGTGCTGGAGTTGA+AGG + tig0013921:5301-5320 Msa1353680:CDS 45.0%
TGCTGGAGTTGAAGGAGTAA+TGG + tig0013921:5309-5328 Msa1353680:CDS 45.0%
! GAGTAATGGTTGATGCTTGG+TGG + tig0013921:5323-5342 Msa1353680:CDS 45.0%
! GTAATGGTTGATGCTTGGTG+GGG + tig0013921:5325-5344 Msa1353680:CDS 45.0%
!! GAAATGTTGGTGACTCTTGC+AGG + tig0013921:5467-5486 Msa1353680:CDS 45.0%
CTAGCATACCTTTACCTCCA+TGG + tig0013921:5682-5701 Msa1353680:intron 45.0%
ATTTCTTCAAGCACCCATGG+AGG - tig0013921:5699-5718 None:intergenic 45.0%
AATAAGGAAGAACCCTGAGC+TGG + tig0013921:5716-5735 Msa1353680:CDS 45.0%
TGTCTGTGTAAACCAGCTCA+GGG - tig0013921:5731-5750 None:intergenic 45.0%
TTGTCTGTGTAAACCAGCTC+AGG - tig0013921:5732-5751 None:intergenic 45.0%
ACACAGACAAATTAGGGAGG+AGG + tig0013921:5742-5761 Msa1353680:CDS 45.0%
GAATCCCGAATACATCTCGT+TGG + tig0013921:5764-5783 Msa1353680:CDS 45.0%
ACAGCCCAACGAGATGTATT+CGG - tig0013921:5772-5791 None:intergenic 45.0%
TAATCGGAGTAGACCTGAAG+AGG - tig0013921:5825-5844 None:intergenic 45.0%
TCAAGCACCCATGGAGGTAA+AGG - tig0013921:5693-5712 None:intergenic 50.0%
CAGCCCAACGAGATGTATTC+GGG - tig0013921:5771-5790 None:intergenic 50.0%
GATTCAGTGCCTGTTCTAGC+TGG + tig0013921:5792-5811 Msa1353680:CDS 50.0%
GGAGTTCTTCCAGCTAGAAC+AGG - tig0013921:5804-5823 None:intergenic 50.0%
AGCTGGAAGAACTCCTCTTC+AGG + tig0013921:5809-5828 Msa1353680:CDS 50.0%
TCAAGCACCCATGGAGGTAA+AGG - tig0013921:5693-5712 None:intergenic 50.0%
CAGCCCAACGAGATGTATTC+GGG - tig0013921:5771-5790 None:intergenic 50.0%
GATTCAGTGCCTGTTCTAGC+TGG + tig0013921:5792-5811 Msa1353680:CDS 50.0%
GGAGTTCTTCCAGCTAGAAC+AGG - tig0013921:5804-5823 None:intergenic 50.0%
AGCTGGAAGAACTCCTCTTC+AGG + tig0013921:5809-5828 Msa1353680:CDS 50.0%
! GGTTGATGCTTGGTGGGGTT+TGG + tig0013921:5330-5349 Msa1353680:CDS 55.0%
! GGTTGATGCTTGGTGGGGTT+TGG + tig0013921:5330-5349 Msa1353680:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0013921 gene 4925 5958 4925 ID=Msa1353680;Name=Msa1353680
tig0013921 mRNA 4925 5958 4925 ID=Msa1353680-mRNA-1;Parent=Msa1353680;Name=Msa1353680-mRNA-1;_AED=0.00;_eAED=0.00;_QI=325|1|1|1|1|1|2|17|164
tig0013921 exon 4925 5488 4925 ID=Msa1353680-mRNA-1:exon:8770;Parent=Msa1353680-mRNA-1
tig0013921 exon 5686 5958 5686 ID=Msa1353680-mRNA-1:exon:8771;Parent=Msa1353680-mRNA-1
tig0013921 five_prime_UTR 4925 5249 4925 ID=Msa1353680-mRNA-1:five_prime_utr;Parent=Msa1353680-mRNA-1
tig0013921 CDS 5250 5488 5250 ID=Msa1353680-mRNA-1:cds;Parent=Msa1353680-mRNA-1
tig0013921 CDS 5686 5941 5686 ID=Msa1353680-mRNA-1:cds;Parent=Msa1353680-mRNA-1
tig0013921 three_prime_UTR 5942 5958 5942 ID=Msa1353680-mRNA-1:three_prime_utr;Parent=Msa1353680-mRNA-1
Gene Sequence

>Msa1353680

ATGGGAGGAAAATTGAATAAAGCAAGAGCAATGAATGCTAGTTTGATGGCTTTGAAGAGTGCTGGAGTTGAAGGAGTAATGGTTGATGCTTGGTGGGGTTTGGTTGAAAAAGATGGTCCTATGAAGTATAATTGGGAAGCTTATGCTGAGCTTGTTCAAATGGTACAAAAGCATGGTTTGAAGCTTCAAATTGTTATGTCTTTTCATCAATGTGGTGGAAATGTTGGTGACTCTTGCAGCATACCTTTACCTCCATGGGTGCTTGAAGAAATAAGGAAGAACCCTGAGCTGGTTTACACAGACAAATTAGGGAGGAGGAATCCCGAATACATCTCGTTGGGCTGTGATTCAGTGCCTGTTCTAGCTGGAAGAACTCCTCTTCAGGTCTACTCCGATTACATGAGAAGCTTCCGTGATAGATTCACAGATTATTTGGGCAATGTCATCATAGTAAGTTTCACCAACTTGGTCTTCAATTTTGGGGTTACTTGGTAA
Protein sequence

>Msa1353680

MGGKLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEAYAELVQMVQKHGLKLQIVMSFHQCGGNVGDSCSIPLPPWVLEEIRKNPELVYTDKLGRRNPEYISLGCDSVPVLAGRTPLQVYSDYMRSFRDRFTDYLGNVIIVSFTNLVFNFGVTW*