AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1359940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1359940 MtrunA17_Chr3g0140531 77.604 192 3 1 1 152 1 192 1.20e-98 291
Msa1359940 MtrunA17_Chr1g0146331 58.659 179 34 1 14 152 10 188 2.11e-66 208
Msa1359940 MtrunA17_Chr1g0146391 58.659 179 34 1 14 152 2 180 2.34e-65 206
Msa1359940 MtrunA17_Chr2g0293331 58.757 177 33 1 16 152 4 180 6.92e-65 204
Msa1359940 MtrunA17_Chr1g0146411 46.632 193 60 3 2 152 9 200 3.40e-52 172
Msa1359940 MtrunA17_Chr1g0146491 46.369 179 56 2 14 152 13 191 3.41e-49 164
Msa1359940 MtrunA17_Chr3g0140761 46.927 179 55 2 14 152 23 201 3.95e-49 164
Msa1359940 MtrunA17_Chr3g0140541 46.354 192 60 2 1 152 5 193 5.51e-48 160
Msa1359940 MtrunA17_Chr8g0384661 45.714 175 55 1 18 152 44 218 4.42e-46 156
Msa1359940 MtrunA17_Chr1g0206821 44.571 175 57 1 18 152 16 190 5.01e-46 157
Msa1359940 MtrunA17_Chr3g0130671 41.270 189 70 2 4 152 20 207 5.44e-46 151
Msa1359940 MtrunA17_Chr3g0125101 43.850 187 64 3 7 152 16 202 8.57e-45 152
Msa1359940 MtrunA17_Chr3g0125081 42.781 187 66 2 7 152 16 202 1.50e-44 152
Msa1359940 MtrunA17_Chr3g0130641 42.077 183 66 1 10 152 19 201 3.33e-44 151
Msa1359940 MtrunA17_Chr1g0206871 44.118 170 55 1 23 152 14 183 6.14e-44 150
Msa1359940 MtrunA17_Chr3g0130651 41.753 194 71 3 1 152 1 194 7.10e-44 150
Msa1359940 MtrunA17_Chr3g0125091 42.246 187 67 3 7 152 16 202 3.24e-43 148
Msa1359940 MtrunA17_Chr1g0159761 41.935 186 68 1 7 152 10 195 2.59e-42 146
Msa1359940 MtrunA17_Chr3g0103211 41.714 175 62 1 18 152 17 191 5.74e-42 144
Msa1359940 MtrunA17_Chr4g0037201 40.957 188 71 1 5 152 40 227 2.52e-41 144
Msa1359940 MtrunA17_Chr3g0103231 37.500 192 79 2 1 152 1 191 1.72e-40 140
Msa1359940 MtrunA17_Chr3g0103221 40.698 172 62 1 21 152 27 198 2.12e-39 138
Msa1359940 MtrunA17_Chr5g0402151 42.771 166 50 2 27 152 3 163 2.56e-36 126
Msa1359940 MtrunA17_Chr1g0206861 64.286 84 30 0 14 97 11 94 8.34e-36 121
Msa1359940 MtrunA17_Chr1g0206841 54.082 98 42 1 1 98 1 95 6.28e-35 117
Msa1359940 MtrunA17_Chr5g0447221 38.235 170 65 2 23 152 28 197 9.77e-35 125
Msa1359940 MtrunA17_Chr3g0103271 35.938 192 82 2 2 152 5 196 3.35e-33 121
Msa1359940 MtrunA17_Chr3g0103261 48.485 99 50 1 1 99 1 98 2.31e-29 105
Msa1359940 MtrunA17_Chr5g0447231 35.673 171 69 3 23 152 24 194 3.09e-24 97.1
Msa1359940 MtrunA17_Chr1g0146351 59.091 66 26 1 34 99 13 77 4.46e-22 85.1
Msa1359940 MtrunA17_Chr1g0206851 64.000 50 18 0 103 152 57 106 2.40e-14 68.9
Msa1359940 MtrunA17_Chr1g0206831 62.000 50 19 0 103 152 20 69 3.21e-13 65.5
Msa1359940 MtrunA17_Chr3g0103251 48.000 50 26 0 103 152 26 75 4.02e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1359940 AT1G10010.1 46.629 178 55 1 15 152 18 195 6.80e-51 169
Msa1359940 AT1G10010.2 46.629 178 55 1 15 152 19 196 6.86e-51 169
Msa1359940 AT1G58360.1 47.486 179 54 1 14 152 26 204 6.90e-51 169
Msa1359940 AT5G09220.1 46.286 175 54 1 18 152 39 213 5.45e-48 161
Msa1359940 AT5G49630.1 43.500 200 65 3 1 152 1 200 7.38e-46 155
Msa1359940 AT1G77380.1 42.458 179 63 1 14 152 19 197 5.11e-45 153
Msa1359940 AT5G63850.1 46.286 175 54 2 18 152 12 186 1.41e-44 152
Msa1359940 AT1G44100.1 41.304 184 68 1 9 152 12 195 8.54e-43 147
Msa1359940 AT5G23810.5 39.773 176 63 3 20 152 21 196 4.88e-36 124
Msa1359940 AT5G23810.3 39.773 176 63 3 20 152 21 196 4.88e-36 124
Msa1359940 AT5G23810.4 39.773 176 63 3 20 152 21 196 6.28e-36 125
Msa1359940 AT5G23810.2 39.773 176 63 3 20 152 21 196 4.32e-35 125
Msa1359940 AT5G23810.1 39.773 176 63 3 20 152 21 196 2.64e-34 125

Find 70 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCAACATTATCGTAGTTT+TGG 0.199946 tig0016862:+2906 Msa1359940:intergenic
ATATATTATGTCATGTCTTT+TGG 0.227629 tig0016862:-2623 Msa1359940:five_prime_UTR
AATCTGAATCTTTATCTAAA+TGG 0.241916 tig0016862:-1680 Msa1359940:exon
AATCCCTACATGCTCGGTTT+TGG 0.254191 tig0016862:-90 Msa1359940:CDS
GCCTGCTGATACGGCAAATA+AGG 0.254571 tig0016862:-2869 Msa1359940:five_prime_UTR
TTCATTTAGCGCAAAATATA+TGG 0.259736 tig0016862:+3019 None:intergenic
TGACGCTTTGGTGAGTTATA+AGG 0.303192 tig0016862:+2677 None:intergenic
ACAAGATAAAATAGTCTCTT+TGG 0.315530 tig0016862:+2749 None:intergenic
GTTGCTGCAATTACCTCTTT+TGG 0.324708 tig0016862:-6 None:intergenic
AGATATCCAGACTCAGTTAA+TGG 0.341029 tig0016862:-831 Msa1359940:CDS
CTCTTGCCATTAACTGAGTC+TGG 0.343732 tig0016862:+825 Msa1359940:intergenic
TATGTCAATGTCAGTGGTAT+TGG 0.352491 tig0016862:+3096 Msa1359940:intergenic
TATCTAAATGGAGGCTGAAA+AGG 0.368758 tig0016862:-1668 Msa1359940:exon
TAATATATTTGTAGTTATAC+AGG 0.373732 tig0016862:+2638 Msa1359940:intergenic
ATTGTAAGTGTAAATAGAAA+TGG 0.388984 tig0016862:+872 Msa1359940:intergenic
TAAACAATGATCATGTTTAG+AGG 0.401005 tig0016862:+3164 Msa1359940:intergenic
TCCTTATTTGCCGTATCAGC+AGG 0.410318 tig0016862:+2868 Msa1359940:intergenic
TGCTAAGTTCACCGGAGAAC+AGG 0.415496 tig0016862:-2494 Msa1359940:intron
AGTAATAATACTCATGGTAT+TGG 0.417123 tig0016862:+3057 Msa1359940:intergenic
GGTGGTGGGTGCAGGAGTGT+TGG 0.440169 tig0016862:-961 Msa1359940:CDS
GTACATTGCAAGTGACGCTT+TGG 0.442042 tig0016862:+2665 Msa1359940:intergenic
CAGGAGTGTTGGCTCTAGCA+TGG 0.444476 tig0016862:-950 Msa1359940:CDS
TTTGATTAAAACTAGTTAGT+GGG 0.452131 tig0016862:+3127 Msa1359940:intergenic
ATAACAGTGGTGGTGGGTGC+AGG 0.463122 tig0016862:-969 Msa1359940:CDS
ATTTGATTAAAACTAGTTAG+TGG 0.464634 tig0016862:+3126 Msa1359940:intergenic
AAACTCAAAGCCTGCTGATA+CGG 0.469154 tig0016862:-2878 Msa1359940:five_prime_UTR
CAAATAAGGATCCATTTACT+TGG 0.469419 tig0016862:-2855 Msa1359940:five_prime_UTR
CTAGTTAGTGGGGAGGGGAT+AGG 0.475973 tig0016862:+3138 Msa1359940:intergenic
GATGGAAGAATCAAGAGAAC+CGG 0.479976 tig0016862:-1604 Msa1359940:intron
TCAAATAATCCCTACATGCT+CGG 0.484226 tig0016862:-96 Msa1359940:CDS
ATCTAAATGGAGGCTGAAAA+GGG 0.484463 tig0016862:-1667 Msa1359940:exon
TACGTTACTCCTTTAAAAGC+CGG 0.494313 tig0016862:+2701 Msa1359940:intergenic
ATAATACTCATGGTATTGGT+GGG 0.504700 tig0016862:+3061 Msa1359940:intergenic
ATACCATGAGTATTATTACT+TGG 0.508565 tig0016862:-3054 Msa1359940:five_prime_UTR
ACAGTACTCAAATGAGGAAG+TGG 0.516285 tig0016862:-1632 Msa1359940:CDS
TTAGTTAATTAATTTGAGAG+TGG 0.516430 tig0016862:-1704 Msa1359940:five_prime_UTR
TTGATTAAAACTAGTTAGTG+GGG 0.518840 tig0016862:+3128 Msa1359940:intergenic
AATAATACTCATGGTATTGG+TGG 0.521333 tig0016862:+3060 Msa1359940:intergenic
TTAGTAATTGCTAAGTTCAC+CGG 0.525109 tig0016862:-2502 Msa1359940:five_prime_UTR
TTAAAACTAGTTAGTGGGGA+GGG 0.528583 tig0016862:+3132 Msa1359940:intergenic
GATTGATGATACCTGTTCTC+CGG 0.535214 tig0016862:+2483 Msa1359940:intergenic
GAGAAATTACACTTATATGC+AGG 0.536730 tig0016862:-805 Msa1359940:CDS
GCATGGGCAATTTCTCAACT+AGG 0.542467 tig0016862:-933 Msa1359940:CDS
AATGAGGAAGTGGATGATGA+TGG 0.542692 tig0016862:-1622 Msa1359940:CDS
TCTCAACTAGGATGGATAGC+TGG 0.542950 tig0016862:-921 Msa1359940:CDS
ACACATAATAACAGTGGTGG+TGG 0.543885 tig0016862:-976 Msa1359940:CDS
GAAAACAATTAAATTTCCAC+CGG 0.544642 tig0016862:-2720 Msa1359940:five_prime_UTR
AGTATTATTACTTGGACACT+AGG 0.550657 tig0016862:-3046 Msa1359940:five_prime_UTR
ATGTTGTTTGATTGATTGCA+AGG 0.551837 tig0016862:-2956 Msa1359940:five_prime_UTR
TAAAACTAGTTAGTGGGGAG+GGG 0.555988 tig0016862:+3133 Msa1359940:intergenic
CTTGCTGCAATGATGACAAT+GGG 0.558549 tig0016862:+2986 Msa1359940:intergenic
TGTCCAAGTAATAATACTCA+TGG 0.565263 tig0016862:+3051 Msa1359940:intergenic
GGGCAATTTCTCAACTAGGA+TGG 0.565746 tig0016862:-929 Msa1359940:CDS
AATATATTTGTAGTTATACA+GGG 0.570950 tig0016862:+2639 Msa1359940:intergenic
TATCAAACAGTACTCAAATG+AGG 0.572155 tig0016862:-1638 Msa1359940:CDS
CCCCAAAACTACGATAATGT+TGG 0.583096 tig0016862:-2908 Msa1359940:five_prime_UTR
GGTTGCAGGGAACGTGTTGA+CGG 0.584738 tig0016862:-1006 Msa1359940:intron
AGGAGTGTTGGCTCTAGCAT+GGG 0.591346 tig0016862:-949 Msa1359940:CDS
ATTAAAACTAGTTAGTGGGG+AGG 0.594129 tig0016862:+3131 Msa1359940:intergenic
CTGAATCTTTATCTAAATGG+AGG 0.596128 tig0016862:-1677 Msa1359940:exon
CACATAATAACAGTGGTGGT+GGG 0.606869 tig0016862:-975 Msa1359940:CDS
TCTTGCTGCAATGATGACAA+TGG 0.614910 tig0016862:+2985 Msa1359940:intergenic
TATATATATGTCAATGTCAG+TGG 0.621958 tig0016862:+3090 Msa1359940:intergenic
TAATACTCATGGTATTGGTG+GGG 0.623728 tig0016862:+3062 Msa1359940:intergenic
GTTACTCCTTTAAAAGCCGG+TGG 0.624487 tig0016862:+2704 Msa1359940:intergenic
TATTTGTAGTTATACAGGGG+AGG 0.647401 tig0016862:+2643 Msa1359940:intergenic
TGATCATGTTTAGAGGCAAG+TGG 0.662501 tig0016862:+3171 Msa1359940:intergenic
AACACACATAATAACAGTGG+TGG 0.670192 tig0016862:-979 Msa1359940:CDS
AACAACACACATAATAACAG+TGG 0.695542 tig0016862:-982 Msa1359940:CDS
ATATATTTGTAGTTATACAG+GGG 0.695622 tig0016862:+2640 Msa1359930:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTACTTTTATCAATTAAA+AGG - tig0016862:382-401 Msa1359940:intron 10.0%
!! CATTGATATATATATATATA+TGG + tig0016862:733-752 Msa1359940:intergenic 10.0%
!!! TCTATTTATATTAATTTTTG+TGG - tig0016862:1926-1945 Msa1359940:intron 10.0%
!!! CTATTTATATTAATTTTTGT+GGG - tig0016862:1927-1946 Msa1359940:intron 10.0%
!!! TATTTATATTAATTTTTGTG+GGG - tig0016862:1928-1947 Msa1359940:intron 10.0%
!! AATATATTTGTAGTTATACA+GGG + tig0016862:540-559 Msa1359940:intergenic 15.0%
!! TAATATATTTGTAGTTATAC+AGG + tig0016862:541-560 Msa1359940:intergenic 15.0%
!! AATTACAATATTATTGACAT+GGG + tig0016862:959-978 Msa1359940:intergenic 15.0%
!! TAATTACAATATTATTGACA+TGG + tig0016862:960-979 Msa1359940:intergenic 15.0%
!! TAAAAAAAAAAACACAATGA+AGG - tig0016862:1199-1218 Msa1359940:intron 15.0%
!! TTTATTGCAAAAAAAAGAAA+AGG + tig0016862:1901-1920 Msa1359940:intergenic 15.0%
!!! TTTCTTTTTTTTGCAATAAA+AGG - tig0016862:1901-1920 Msa1359940:intron 15.0%
!! AGTATATTTGATGTATTTAA+TGG - tig0016862:2119-2138 Msa1359940:intron 15.0%
!! ATGTATTTAATGGAAATAAT+TGG - tig0016862:2129-2148 Msa1359940:intron 15.0%
!!! TTTCAAATGTTTTAATTAGT+TGG - tig0016862:2587-2606 Msa1359940:five_prime_UTR 15.0%
!! ATTAACAATACCATAAATTA+GGG + tig0016862:2987-3006 Msa1359940:intergenic 15.0%
!! TTTGATTAAAACTAGTTAGT+GGG + tig0016862:52-71 Msa1264120:intergenic 20.0%
!! ATTTGATTAAAACTAGTTAG+TGG + tig0016862:53-72 Msa1264120:intergenic 20.0%
!! ATATATTTGTAGTTATACAG+GGG + tig0016862:539-558 Msa1359940:intergenic 20.0%
!!! ATATATTATGTCATGTCTTT+TGG - tig0016862:553-572 Msa1359940:intron 20.0%
!! ATTACAATATTATTGACATG+GGG + tig0016862:958-977 Msa1359940:intergenic 20.0%
!!! TTTGTTTTCATAGAGTTTTT+TGG - tig0016862:1310-1329 Msa1359940:intron 20.0%
!!! TTAGTTAATTAATTTGAGAG+TGG - tig0016862:1472-1491 Msa1359940:intron 20.0%
!! AATCTGAATCTTTATCTAAA+TGG - tig0016862:1496-1515 Msa1359940:intron 20.0%
!! AACACATGAATTATATGAAA+AGG + tig0016862:1658-1677 Msa1359940:intergenic 20.0%
!! TAAAAGAAAACATCTTTCAA+AGG + tig0016862:1742-1761 Msa1359940:intergenic 20.0%
!! AAATTGACAATTTCAATAGA+TGG + tig0016862:1805-1824 Msa1359940:intergenic 20.0%
!! AATGATAGCATTAGTTAATA+TGG + tig0016862:2002-2021 Msa1359940:intergenic 20.0%
!! ATTGTAAGTGTAAATAGAAA+TGG + tig0016862:2307-2326 Msa1359940:intergenic 20.0%
!! TGTATTTATGATATATACCT+AGG + tig0016862:2410-2429 Msa1359940:intergenic 20.0%
!!! TTCTATTAATCAATTTGTTG+AGG + tig0016862:2825-2844 Msa1359940:intergenic 20.0%
!! CATTAACAATACCATAAATT+AGG + tig0016862:2988-3007 Msa1359940:intergenic 20.0%
!! ATGTTAACTTATGGAAATTT+GGG + tig0016862:3139-3158 Msa1359940:intergenic 20.0%
!! AATGTTAACTTATGGAAATT+TGG + tig0016862:3140-3159 Msa1359940:intergenic 20.0%
!! TTGATATCAATGTTAACTTA+TGG + tig0016862:3148-3167 Msa1359940:intergenic 20.0%
! TAAACAATGATCATGTTTAG+AGG + tig0016862:15-34 Msa1264120:intergenic 25.0%
! TTGATTAAAACTAGTTAGTG+GGG + tig0016862:51-70 Msa1264120:intergenic 25.0%
! TATATATATGTCAATGTCAG+TGG + tig0016862:89-108 Msa1264120:intergenic 25.0%
! AGTAATAATACTCATGGTAT+TGG + tig0016862:122-141 Msa1264120:intergenic 25.0%
! ATACCATGAGTATTATTACT+TGG - tig0016862:122-141 Msa1359940:CDS 25.0%
! TTCATTTAGCGCAAAATATA+TGG + tig0016862:160-179 Msa1359940:intergenic 25.0%
!! AAACATTTTCACCAAGTAAA+TGG + tig0016862:335-354 Msa1359940:intergenic 25.0%
! ACAAGATAAAATAGTCTCTT+TGG + tig0016862:430-449 Msa1359940:intergenic 25.0%
!! GGTGGAAATTTAATTGTTTT+CGG + tig0016862:457-476 Msa1359940:intergenic 25.0%
! GAAAACAATTAAATTTCCAC+CGG - tig0016862:456-475 Msa1359940:intron 25.0%
! TATATATATGGGATCAAAGA+AGG + tig0016862:721-740 Msa1359940:intergenic 25.0%
! TAAAGAAAACAGAACAGTAT+TGG + tig0016862:875-894 Msa1359940:intergenic 25.0%
! AAATAAAAAATCCTCCATTG+TGG + tig0016862:1173-1192 Msa1359940:intergenic 25.0%
! AATTAAGAAAAAACTCCATG+AGG - tig0016862:1233-1252 Msa1359940:intron 25.0%
!!! TATTTGTATGTTTGGTTTTC+TGG - tig0016862:1354-1373 Msa1359940:intron 25.0%
! ATGCATCAAAAACAAAATTG+AGG + tig0016862:1418-1437 Msa1359940:intergenic 25.0%
!!! CAATTTTGTTTTTGATGCAT+AGG - tig0016862:1418-1437 Msa1359940:intron 25.0%
! CAAATATACTAGTATCTAAC+TGG + tig0016862:2109-2128 Msa1359940:intergenic 25.0%
! TGGAAATAATTGGATTTGAA+TGG - tig0016862:2139-2158 Msa1359940:intron 25.0%
!!! TTTAGCATTGACAAAAATAG+TGG + tig0016862:2556-2575 Msa1359940:intergenic 25.0%
!!! TTGATTAACCAGAGTATTTT+TGG - tig0016862:2732-2751 Msa1359940:five_prime_UTR 25.0%
! AAATCATACTCCCTAATTTA+TGG - tig0016862:2974-2993 Msa1359940:five_prime_UTR 25.0%
! GTATTGTTAATGTATACATG+TGG - tig0016862:2996-3015 Msa1359940:five_prime_UTR 25.0%
!! TTTGAGACAATAAAATCTGA+AGG + tig0016862:3115-3134 Msa1359940:intergenic 25.0%
!! ATAATACTCATGGTATTGGT+GGG + tig0016862:118-137 Msa1264120:intergenic 30.0%
! AATAATACTCATGGTATTGG+TGG + tig0016862:119-138 Msa1264120:intergenic 30.0%
TGTCCAAGTAATAATACTCA+TGG + tig0016862:128-147 Msa1359940:intergenic 30.0%
AGTATTATTACTTGGACACT+AGG - tig0016862:130-149 Msa1359940:CDS 30.0%
! ATGTTGTTTGATTGATTGCA+AGG - tig0016862:220-239 Msa1359940:intron 30.0%
CAAATAAGGATCCATTTACT+TGG - tig0016862:321-340 Msa1359940:intron 30.0%
TTAGTAATTGCTAAGTTCAC+CGG - tig0016862:674-693 Msa1359940:intron 30.0%
AGACACGATATTTGTATGTT+TGG - tig0016862:1346-1365 Msa1359940:intron 30.0%
! TGGTTTGAAACATGTTGATT+AGG - tig0016862:1374-1393 Msa1359940:intron 30.0%
CTGAATCTTTATCTAAATGG+AGG - tig0016862:1499-1518 Msa1359940:intron 30.0%
TATCAAACAGTACTCAAATG+AGG - tig0016862:1538-1557 Msa1359940:intron 30.0%
GAAATGAAATGAATGCATAC+CGG + tig0016862:1594-1613 Msa1359940:intergenic 30.0%
! AAGGACATATGATTCTGAAA+TGG + tig0016862:1639-1658 Msa1359940:intergenic 30.0%
ACGTCATGAAAGAAAATAAG+AGG + tig0016862:1868-1887 Msa1359940:intergenic 30.0%
! TTTTCTTTCATGACGTCAAA+CGG - tig0016862:1872-1891 Msa1359940:intron 30.0%
GTTGAAGGAGTAATAGAATT+TGG - tig0016862:1958-1977 Msa1359940:intron 30.0%
AACAACACACATAATAACAG+TGG - tig0016862:2194-2213 Msa1359940:intron 30.0%
GAGAAATTACACTTATATGC+AGG - tig0016862:2371-2390 Msa1359940:intron 30.0%
!! AGTACAATTTTAGCGTGAAT+CGG + tig0016862:2506-2525 Msa1359940:intergenic 30.0%
!! TTTTGTGGATTGATTCAGTA+TGG - tig0016862:2642-2661 Msa1359940:five_prime_UTR 30.0%
! TTTGTGGATTGATTCAGTAT+GGG - tig0016862:2643-2662 Msa1359940:five_prime_UTR 30.0%
!!! TTTTAGGACCAAAAATACTC+TGG + tig0016862:2743-2762 Msa1359940:intergenic 30.0%
TTGCAATTTCAATGCATTCA+AGG + tig0016862:2798-2817 Msa1359940:intergenic 30.0%
!! TTGATTTGCTTCCACAAAAA+TGG - tig0016862:3035-3054 Msa1359940:five_prime_UTR 30.0%
AAAACTTGCAATAAGCTTCA+TGG + tig0016862:3061-3080 Msa1359940:intergenic 30.0%
TTAAAACTAGTTAGTGGGGA+GGG + tig0016862:47-66 Msa1264120:intergenic 35.0%
ATTAAAACTAGTTAGTGGGG+AGG + tig0016862:48-67 Msa1264120:intergenic 35.0%
TATGTCAATGTCAGTGGTAT+TGG + tig0016862:83-102 Msa1264120:intergenic 35.0%
!! TAATACTCATGGTATTGGTG+GGG + tig0016862:117-136 Msa1264120:intergenic 35.0%
!! CCAACATTATCGTAGTTTTG+GGG + tig0016862:271-290 Msa1359940:intergenic 35.0%
! GCCAACATTATCGTAGTTTT+GGG + tig0016862:272-291 Msa1359940:intergenic 35.0%
! TGCCAACATTATCGTAGTTT+TGG + tig0016862:273-292 Msa1359940:intergenic 35.0%
!!! AAATTTCCACCGGCTTTTAA+AGG - tig0016862:466-485 Msa1359940:intron 35.0%
! TACGTTACTCCTTTAAAAGC+CGG + tig0016862:478-497 Msa1359940:intergenic 35.0%
TATTTGTAGTTATACAGGGG+AGG + tig0016862:536-555 Msa1359940:intergenic 35.0%
ACACGTAACTAACACTGTTT+CGG - tig0016862:804-823 Msa1359940:CDS 35.0%
! TTCTTTACAGTCTTGTGTTG+TGG - tig0016862:888-907 Msa1359940:CDS 35.0%
!! CATTTAATTTGCACCACATG+TGG - tig0016862:1122-1141 Msa1359940:intron 35.0%
!! AGAATTTGTTAGACCACATG+TGG + tig0016862:1138-1157 Msa1359940:intergenic 35.0%
! ATAGGCTCTAATTCTTGACA+GGG - tig0016862:1436-1455 Msa1359940:intron 35.0%
TATCTAAATGGAGGCTGAAA+AGG - tig0016862:1508-1527 Msa1359940:intron 35.0%
ATCTAAATGGAGGCTGAAAA+GGG - tig0016862:1509-1528 Msa1359940:intron 35.0%
TTTCTTTCATGACGTCAAAC+GGG - tig0016862:1873-1892 Msa1359940:intron 35.0%
AACACACATAATAACAGTGG+TGG - tig0016862:2197-2216 Msa1359940:intron 35.0%
AGATATCCAGACTCAGTTAA+TGG - tig0016862:2345-2364 Msa1359940:intron 35.0%
TATACCTAGGTAAGCATGAA+CGG + tig0016862:2397-2416 Msa1359940:intergenic 35.0%
AAGTCAAGTCGGATAAATGT+GGG + tig0016862:2455-2474 Msa1359940:intergenic 35.0%
! ATTTATCCGACTTGACTTGA+GGG - tig0016862:2457-2476 Msa1359940:intron 35.0%
ATTAATCCCTCAAGTCAAGT+CGG + tig0016862:2466-2485 Msa1359940:intergenic 35.0%
!! GTACAATTTTAGCGTGAATC+GGG + tig0016862:2505-2524 Msa1359940:intergenic 35.0%
!! TAGTTGGCCATTTTGTCATT+AGG - tig0016862:2603-2622 Msa1359940:five_prime_UTR 35.0%
TGTTCCACCTAATGACAAAA+TGG + tig0016862:2613-2632 Msa1359940:intergenic 35.0%
! GGAACAATGCACATATTTTG+TGG - tig0016862:2627-2646 Msa1359940:five_prime_UTR 35.0%
!! TATAACTTGCTCTACAAGCA+TGG - tig0016862:2695-2714 Msa1359940:five_prime_UTR 35.0%
! CAACCTCACTCACATATTTT+AGG + tig0016862:2759-2778 Msa1359940:intergenic 35.0%
CTAAAATATGTGAGTGAGGT+TGG - tig0016862:2757-2776 Msa1359940:five_prime_UTR 35.0%
TGTTAATGTATACATGTGGC+AGG - tig0016862:3000-3019 Msa1359940:five_prime_UTR 35.0%
GTTAATGTATACATGTGGCA+GGG - tig0016862:3001-3020 Msa1359940:five_prime_UTR 35.0%
TCAAATAATCCCTACATGCT+CGG - tig0016862:3080-3099 Msa1359940:five_prime_UTR 35.0%
TAAAACTAGTTAGTGGGGAG+GGG + tig0016862:46-65 Msa1264120:intergenic 40.0%
CTTGCTGCAATGATGACAAT+GGG + tig0016862:193-212 Msa1359940:intergenic 40.0%
TCTTGCTGCAATGATGACAA+TGG + tig0016862:194-213 Msa1359940:intergenic 40.0%
CCCCAAAACTACGATAATGT+TGG - tig0016862:268-287 Msa1359940:intron 40.0%
AAACTCAAAGCCTGCTGATA+CGG - tig0016862:298-317 Msa1359940:intron 40.0%
TGACGCTTTGGTGAGTTATA+AGG + tig0016862:502-521 Msa1359940:intergenic 40.0%
! GATTGATGATACCTGTTCTC+CGG + tig0016862:696-715 Msa1359940:intergenic 40.0%
!! GAAAACAGAACAGTATTGGC+AGG + tig0016862:871-890 Msa1359940:intergenic 40.0%
! TTTACAGTCTTGTGTTGTGG+TGG - tig0016862:891-910 Msa1359940:CDS 40.0%
!! ATCCTCCATTGTGGTTTGTT+AGG + tig0016862:1164-1183 Msa1359940:intergenic 40.0%
! CATAGGCTCTAATTCTTGAC+AGG - tig0016862:1435-1454 Msa1359940:intron 40.0%
ACAGTACTCAAATGAGGAAG+TGG - tig0016862:1544-1563 Msa1359940:intron 40.0%
AATGAGGAAGTGGATGATGA+TGG - tig0016862:1554-1573 Msa1359940:intron 40.0%
GATGGAAGAATCAAGAGAAC+CGG - tig0016862:1572-1591 Msa1359940:intron 40.0%
TTCTTTCATGACGTCAAACG+GGG - tig0016862:1874-1893 Msa1359940:intron 40.0%
TGGATTTGAATGGATGCTTG+TGG - tig0016862:2149-2168 Msa1359940:intron 40.0%
ACACATAATAACAGTGGTGG+TGG - tig0016862:2200-2219 Msa1359940:intron 40.0%
CACATAATAACAGTGGTGGT+GGG - tig0016862:2201-2220 Msa1359940:intron 40.0%
CAAGTCAAGTCGGATAAATG+TGG + tig0016862:2456-2475 Msa1359940:intergenic 40.0%
! CATTTATCCGACTTGACTTG+AGG - tig0016862:2456-2475 Msa1359940:intron 40.0%
GACTTGAGGGATTAATTGCA+CGG - tig0016862:2470-2489 Msa1359940:intron 40.0%
!! TTGGCCATTTTGTCATTAGG+TGG - tig0016862:2606-2625 Msa1359940:five_prime_UTR 40.0%
GGTCCTAAAATATGTGAGTG+AGG - tig0016862:2753-2772 Msa1359940:five_prime_UTR 40.0%
! TGAGGTTGGTTGTATTACTG+TGG - tig0016862:2771-2790 Msa1359940:five_prime_UTR 40.0%
!!! AAGCTTCATGGCCATTTTTG+TGG + tig0016862:3049-3068 Msa1359940:intergenic 40.0%
! TCCTTATTTGCCGTATCAGC+AGG + tig0016862:311-330 Msa1359940:intergenic 45.0%
! GTTACTCCTTTAAAAGCCGG+TGG + tig0016862:475-494 Msa1359940:intergenic 45.0%
GTACATTGCAAGTGACGCTT+TGG + tig0016862:514-533 Msa1359940:intergenic 45.0%
GCACGATTGGAATTTGGACA+GGG + tig0016862:849-868 Msa1359940:intergenic 45.0%
CTGCACCTAACAAACCACAA+TGG - tig0016862:1156-1175 Msa1359940:intron 45.0%
CACCTAACAAACCACAATGG+AGG - tig0016862:1159-1178 Msa1359940:intron 45.0%
ACCACATTCACAGAGAAGGA+GGG - tig0016862:1256-1275 Msa1359940:intron 45.0%
GACGTACGATATTGCTGCAA+CGG - tig0016862:1282-1301 Msa1359940:intron 45.0%
! TTGTGGGGATTGTGAGTTGA+AGG - tig0016862:1943-1962 Msa1359940:intron 45.0%
AATGGATGCTTGTGGTTGCA+GGG - tig0016862:2157-2176 Msa1359940:intron 45.0%
GCATGGGCAATTTCTCAACT+AGG - tig0016862:2243-2262 Msa1359940:intron 45.0%
GGGCAATTTCTCAACTAGGA+TGG - tig0016862:2247-2266 Msa1359940:intron 45.0%
TCTCAACTAGGATGGATAGC+TGG - tig0016862:2255-2274 Msa1359940:intron 45.0%
CTCTTGCCATTAACTGAGTC+TGG + tig0016862:2354-2373 Msa1359940:intergenic 45.0%
ATTCAGTATGGGAAACTCGC+TGG - tig0016862:2654-2673 Msa1359940:five_prime_UTR 45.0%
TTCAGTATGGGAAACTCGCT+GGG - tig0016862:2655-2674 Msa1359940:five_prime_UTR 45.0%
AAACTCGCTGGGATTACAGT+GGG - tig0016862:2666-2685 Msa1359940:five_prime_UTR 45.0%
! AATCCCTACATGCTCGGTTT+TGG - tig0016862:3086-3105 Msa1359940:five_prime_UTR 45.0%
! ATCCCTACATGCTCGGTTTT+GGG - tig0016862:3087-3106 Msa1359940:five_prime_UTR 45.0%
ATCCCAAAACCGAGCATGTA+GGG + tig0016862:3092-3111 Msa1359940:intergenic 45.0%
!! ATTGATATATATATATATAT+GGG + tig0016862:732-751 Msa1359940:intergenic 5.0%
!!! TCTTTTAAAATAATTTATTT+TGG - tig0016862:1755-1774 Msa1359940:intron 5.0%
GCCTGCTGATACGGCAAATA+AGG - tig0016862:307-326 Msa1359940:intron 50.0%
TGCTAAGTTCACCGGAGAAC+AGG - tig0016862:682-701 Msa1359940:intron 50.0%
GGCACGATTGGAATTTGGAC+AGG + tig0016862:850-869 Msa1359940:intergenic 50.0%
TGGCAGGCACGATTGGAATT+TGG + tig0016862:855-874 Msa1359940:intergenic 50.0%
!! ACAGTATTGGCAGGCACGAT+TGG + tig0016862:862-881 Msa1359940:intergenic 50.0%
TTATTGACATGGGGCCAGTG+AGG + tig0016862:949-968 Msa1359940:intergenic 50.0%
CTCTGTGAATGTGGTCCTCA+TGG + tig0016862:1251-1270 Msa1359940:intergenic 50.0%
GAGGACCACATTCACAGAGA+AGG - tig0016862:1252-1271 Msa1359940:intron 50.0%
GACCACATTCACAGAGAAGG+AGG - tig0016862:1255-1274 Msa1359940:intron 50.0%
CCACATTCACAGAGAAGGAG+GGG - tig0016862:1257-1276 Msa1359940:intron 50.0%
CACATTCACAGAGAAGGAGG+GGG - tig0016862:1258-1277 Msa1359940:intron 50.0%
GAATGGATGCTTGTGGTTGC+AGG - tig0016862:2156-2175 Msa1359940:intron 50.0%
!! AGGAGTGTTGGCTCTAGCAT+GGG - tig0016862:2227-2246 Msa1359940:intron 50.0%
GAAACTCGCTGGGATTACAG+TGG - tig0016862:2665-2684 Msa1359940:five_prime_UTR 50.0%
GATCCCAAAACCGAGCATGT+AGG + tig0016862:3093-3112 Msa1359940:intergenic 50.0%
CTAGTTAGTGGGGAGGGGAT+AGG + tig0016862:41-60 Msa1264120:intergenic 55.0%
CCCCTCCTTCTCTGTGAATG+TGG + tig0016862:1260-1279 Msa1359940:intergenic 55.0%
GGTTGCAGGGAACGTGTTGA+CGG - tig0016862:2170-2189 Msa1359940:intron 55.0%
!! ATAACAGTGGTGGTGGGTGC+AGG - tig0016862:2207-2226 Msa1359940:intron 55.0%
!! CAGGAGTGTTGGCTCTAGCA+TGG - tig0016862:2226-2245 Msa1359940:intron 55.0%
CAGGCCGTTCATGCTTACCT+AGG - tig0016862:2390-2409 Msa1359940:intron 55.0%
TCACCCTCACCTCACCTCAC+TGG - tig0016862:932-951 Msa1359940:CDS 60.0%
GGGCCAGTGAGGTGAGGTGA+GGG + tig0016862:938-957 Msa1359940:intergenic 65.0%
GACATGGGGCCAGTGAGGTG+AGG + tig0016862:944-963 Msa1359940:intergenic 65.0%
!! GGTGGTGGGTGCAGGAGTGT+TGG - tig0016862:2215-2234 Msa1359940:intron 65.0%
GGGGCCAGTGAGGTGAGGTG+AGG + tig0016862:939-958 Msa1359940:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0016862 gene 8 3190 8 ID=Msa1359940;Name=Msa1359940
tig0016862 mRNA 8 3190 8 ID=Msa1359940-mRNA-1;Parent=Msa1359940;Name=Msa1359940-mRNA-1;_AED=0.23;_eAED=0.23;_QI=754|0.66|0.5|1|0.33|0|4|0|152
tig0016862 exon 8 166 8 ID=Msa1359940-mRNA-1:exon:11637;Parent=Msa1359940-mRNA-1
tig0016862 exon 803 1020 803 ID=Msa1359940-mRNA-1:exon:11636;Parent=Msa1359940-mRNA-1
tig0016862 exon 1605 1741 1605 ID=Msa1359940-mRNA-1:exon:11635;Parent=Msa1359940-mRNA-1
tig0016862 exon 2495 3190 2495 ID=Msa1359940-mRNA-1:exon:11634;Parent=Msa1359940-mRNA-1
tig0016862 five_prime_UTR 2495 3190 2495 ID=Msa1359940-mRNA-1:five_prime_utr;Parent=Msa1359940-mRNA-1
tig0016862 five_prime_UTR 1684 1741 1684 ID=Msa1359940-mRNA-1:five_prime_utr;Parent=Msa1359940-mRNA-1
tig0016862 CDS 1605 1683 1605 ID=Msa1359940-mRNA-1:cds;Parent=Msa1359940-mRNA-1
tig0016862 CDS 803 1020 803 ID=Msa1359940-mRNA-1:cds;Parent=Msa1359940-mRNA-1
tig0016862 CDS 8 166 8 ID=Msa1359940-mRNA-1:cds;Parent=Msa1359940-mRNA-1
Gene Sequence

>Msa1359940

ATGGAGGCTGAAAAGGGTGTGTCTATCAAACAGTACTCAAATGAGGAAGTGGATGATGATGGAAGAATCAAGAGAACCGGGAACGTGTTGACGGCAACAACACACATAATAACAGTGGTGGTGGGTGCAGGAGTGTTGGCTCTAGCATGGGCAATTTCTCAACTAGGATGGATAGCTGGCATACTTGTTATGATCACATTTTCATCCATTTCTATTTACACTTACAATCTTATAGCTGATTGTTATAGATATCCAGACTCAGTTAATGGCAAGAGAAATTACACTTATATGCAGGCCAAATTGATTTGCTTCCACAAAAATGGCCATGAAGCTTATTGCAAGTTTTCAAATAATCCCTACATGCTCGGTTTTGGGATCCTTCAGATTTTATTGTCTCAAATCCCAAATTTCCATAAGTTAACATTGATATCAACTGTTGCTGCAATTACCTCTTTT
Protein sequence

>Msa1359940

MEAEKGVSIKQYSNEEVDDDGRIKRTGNVLTATTHIITVVVGAGVLALAWAISQLGWIAGILVMITFSSISIYTYNLIADCYRYPDSVNGKRNYTYMQAKLICFHKNGHEAYCKFSNNPYMLGFGILQILLSQIPNFHKLTLISTVAAITSF