AlfalfaGEDB | Msa1367340

M. sativa cultivar zhongmu-4 / Msa1367340

KEGG: -
GO: -
PFAM: PF13499.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1367340	XP_003604022.1	97.521	363	8	1	1	363	1	362	0.0	710

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1367340	G7J7U7	97.521	363	8	1	1	363	1	362	0.0	710

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0380240	Msa1367340	0.895110	1.308327e-75	-8.615850e-47
Msa0505370	Msa1367340	0.802900	4.504987e-49	-8.615850e-47
Msa0524160	Msa1367340	0.944129	3.430678e-103	-8.615850e-47
Msa1361010	Msa1367340	0.810275	1.250022e-50	-8.615850e-47
Msa1365090	Msa1367340	0.969833	1.044257e-130	-8.615850e-47
Msa1367340	Msa1443080	0.960043	4.104882e-118	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1367340	MtrunA17_Chr3g0145431	97.521	363	8	1	1	363	1	362	0.0	710
Msa1367340	MtrunA17_Chr4g0038631	73.569	367	87	4	4	363	7	370	0.0	535

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1367340	AT4G38810.2	64.407	354	117	4	12	359	13	363	3.74e-161	456
Msa1367340	AT4G38810.1	58.984	256	96	4	110	359	1	253	3.54e-100	297
Msa1367340	AT5G28830.1	23.102	303	205	8	54	346	39	323	1.15e-11	65.5

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Find 95 sgRNAs with CRISPR-Local

Find 308 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTTGAGACAACAGTGC+CGG	0.602253	tig0018513:-29560	None:intergenic
AACAAGAATAATATTAAGAT+GGG	0.524551	tig0018513:-30555	None:intergenic
AACCAGAAGAGGAACGGAGG+AGG	0.517853	tig0018513:+26168	Msa1367340:CDS
AAGCTTCAATAACAAGTTCG+CGG	0.551585	tig0018513:-29266	None:intergenic
AATATCAAAGGATTACTTGC+AGG	0.400730	tig0018513:+29888	Msa1367340:CDS
AATCTGCCAAAAGATCGAAA+CGG	0.663483	tig0018513:+29862	Msa1367340:CDS
AATGTCTCTTGATCTCTAAC+AGG	0.406167	tig0018513:-29590	None:intergenic
ACCCAAGAATCTGATGTAGG+AGG	0.614585	tig0018513:-29318	None:intergenic
ACCTCTTCAATTGCACCAAC+TGG	0.390172	tig0018513:-29949	None:intergenic
ACTGGAGGTAAACCAGCAGA+TGG	0.614242	tig0018513:-29931	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCACATACACAAAAAGAAA+GGG	-	tig0018513:26679-26698	None:intergenic	30.0%
AACCACATACACAAAAAGAA+AGG	-	tig0018513:26680-26699	None:intergenic	30.0%
ATGTGATACTTTATAGAAGC+TGG	+	tig0018513:26808-26827	Msa1367340:intron	30.0%
ATTATCCATGATAACATGGT+CGG	+	tig0018513:26856-26875	Msa1367340:intron	30.0%
AGACACACTAATGAAAAAAC+CGG	-	tig0018513:27141-27160	None:intergenic	30.0%
AGTATATGCTAAACCATTGA+CGG	+	tig0018513:27198-27217	Msa1367340:intron	30.0%
TCAGTTAGCACTAGTAATTT+GGG	-	tig0018513:27371-27390	None:intergenic	30.0%
ATCAGTTAGCACTAGTAATT+TGG	-	tig0018513:27372-27391	None:intergenic	30.0%
GTGCTCAGATAATTGATAAA+TGG	+	tig0018513:27544-27563	Msa1367340:intron	30.0%
TTTCCATGAGATATCTATCT+AGG	+	tig0018513:27918-27937	Msa1367340:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0018513	gene	26018	30748	26018	ID=Msa1367340;Name=Msa1367340
tig0018513	mRNA	26018	30748	26018	ID=Msa1367340-mRNA-1;Parent=Msa1367340;Name=Msa1367340-mRNA-1;_AED=0.12;_eAED=0.12;_QI=144\|1\|1\|1\|1\|1\|5\|184\|363
tig0018513	exon	26018	26404	26018	ID=Msa1367340-mRNA-1:exon:15507;Parent=Msa1367340-mRNA-1
tig0018513	exon	29042	29338	29042	ID=Msa1367340-mRNA-1:exon:15508;Parent=Msa1367340-mRNA-1
tig0018513	exon	29539	29751	29539	ID=Msa1367340-mRNA-1:exon:15509;Parent=Msa1367340-mRNA-1
tig0018513	exon	29841	29969	29841	ID=Msa1367340-mRNA-1:exon:15510;Parent=Msa1367340-mRNA-1
tig0018513	exon	30355	30748	30355	ID=Msa1367340-mRNA-1:exon:15511;Parent=Msa1367340-mRNA-1
tig0018513	five_prime_UTR	26018	26161	26018	ID=Msa1367340-mRNA-1:five_prime_utr;Parent=Msa1367340-mRNA-1
tig0018513	CDS	26162	26404	26162	ID=Msa1367340-mRNA-1:cds;Parent=Msa1367340-mRNA-1
tig0018513	CDS	29042	29338	29042	ID=Msa1367340-mRNA-1:cds;Parent=Msa1367340-mRNA-1
tig0018513	CDS	29539	29751	29539	ID=Msa1367340-mRNA-1:cds;Parent=Msa1367340-mRNA-1
tig0018513	CDS	29841	29969	29841	ID=Msa1367340-mRNA-1:cds;Parent=Msa1367340-mRNA-1
tig0018513	CDS	30355	30564	30355	ID=Msa1367340-mRNA-1:cds;Parent=Msa1367340-mRNA-1
tig0018513	three_prime_UTR	30565	30748	30565	ID=Msa1367340-mRNA-1:three_prime_utr;Parent=Msa1367340-mRNA-1

Gene Sequence

>Msa1367340

ATGGAAAACCAGAAGAGGAACGGAGGAGGAGGAGAGGTTCTTGATGGCTCCAACATTATGGAATTGGTTGGAAACGAGCAAGGATTCAACAAGTTTGTGGACCACAAATTTCATGAGCTGGATAAGGACAGAGATGGTAAGCTCTCCTTGAAGGAGCTTGAGCCCGCTGTTGCTGACATCGGTGCTGCTCTTGGTTTGCCTGCTCAGGGCACTACTCCTGACTCTGATCACATCTACTATCAGGTGTTGAATGAATTCACTCATGGAAAGCAAGAAAAAGTGAGCAAGAGTGAGTTCAAAGAGGTTCTCTCAGACATTCTATTAGGCATGGCTGCTGGACTGAAGCGAGACCCAATTGTTATACTCTGCATGGATGGGGAAGATCTTCTTGAGTTTGTTAACGGTCCAAGTTATTATACAGAAATGACATCCATATTCTCTCAGATTCAAAACTCTAGTACATCACTCCGCGAACTTGTTATTGAAGCTTTTGGAAGACTCGATGTTGATCGAGGAATTCCTCCTACATCAGATTCTTGGGTTTTTAACAACATTGTGGATCCGGCACTGTTGTCTCAAGCTTTGGATAGGCCTGTTAGAGATCAAGAGACATTTTTGGAAGAATTCAAGAAAGTGGCATTGAGTGTGGTTGATTGCCTTAAAGAGAAGCCTGTCATTGTAGCTCACAGTGAAAACACTTTTGATGGAAGTGGTGTCAAGAGACTCTTATCCAACAAGTTTGAATTAGAAAAGACGTTGAACACGACTTTAGGGAATCTGCCAAAAGATCGAAACGGAAAAATATCAAAGGATTACTTGCAGGTGACACTAGATGTGGTGTCTCCATCTGCTGGTTTACCTCCAGTTGGTGCAATTGAAGAGATGGATAAGGTTATTAGTGAAGCCTTTAAGACGGTGAATAATGAAGATGACAAGAAGATGATGATTAAAGAAGAAGAATTCAAGAAAATTTTAATTGAAATACTGGGGAGTATCATGTTGCAGTTAGAAGGAAGTCCCATTGCTGTTTCTTCAAATTCAGTTTTACACGAGCCTTTAAGCTCATCTTCTACACTTTTGAACCCATCTTAA

Protein sequence

>Msa1367340

MENQKRNGGGGEVLDGSNIMELVGNEQGFNKFVDHKFHELDKDRDGKLSLKELEPAVADIGAALGLPAQGTTPDSDHIYYQVLNEFTHGKQEKVSKSEFKEVLSDILLGMAAGLKRDPIVILCMDGEDLLEFVNGPSYYTEMTSIFSQIQNSSTSLRELVIEAFGRLDVDRGIPPTSDSWVFNNIVDPALLSQALDRPVRDQETFLEEFKKVALSVVDCLKEKPVIVAHSENTFDGSGVKRLLSNKFELEKTLNTTLGNLPKDRNGKISKDYLQVTLDVVSPSAGLPPVGAIEEMDKVISEAFKTVNNEDDKKMMIKEEEFKKILIEILGSIMLQLEGSPIAVSSNSVLHEPLSSSSTLLNPS*