AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1368630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTCTGTAGTGGTTGTTTC+TGG 0.049757 tig0018650:-118501 None:intergenic
TCTATCCTCGAGTTTATATT+TGG 0.181930 tig0018650:+121021 Msa1368630:three_prime_UTR
GGTAGAATATTCACCTAATC+AGG 0.266410 tig0018650:+121245 Msa1368630:three_prime_UTR
AGCTGATTGTTCTGGGAAAT+TGG 0.293695 tig0018650:+120923 Msa1368630:exon
CAGTTACAAGTTTGCATCCT+TGG 0.331082 tig0018650:+120883 Msa1368630:intron
CCAAAATACCTGCGGTAAAT+CGG 0.345611 tig0018650:-118461 None:intergenic
GTTAATTTGTTGGTATGCCT+TGG 0.359869 tig0018650:+121169 Msa1368630:three_prime_UTR
TATGATACTTGTTAATTTGT+TGG 0.367418 tig0018650:+121159 Msa1368630:three_prime_UTR
ATTAGAAGAACGAGAAATAA+AGG 0.372863 tig0018650:-118328 None:intergenic
CCTCTGTAGTGGTTGTTTCT+GGG 0.378764 tig0018650:-118500 None:intergenic
CTAATAGCAGCTCATGTTCT+CGG 0.382026 tig0018650:+118346 Msa1368630:CDS
CAACTCTTATCACTTTATAT+AGG 0.387456 tig0018650:-121412 None:intergenic
TTAATTTGTTGGTATGCCTT+GGG 0.390776 tig0018650:+121170 Msa1368630:three_prime_UTR
GTTATTTGAGAATAGATGTA+AGG 0.422271 tig0018650:-118427 None:intergenic
ATTGCGTCCAGGGTTCAGTT+GGG 0.433906 tig0018650:+118198 Msa1368630:five_prime_UTR
TATTGCGTCCAGGGTTCAGT+TGG 0.448218 tig0018650:+118197 Msa1368630:five_prime_UTR
TAACACATATCACCCTGATT+AGG 0.452705 tig0018650:-121258 None:intergenic
TTTATTTGGGAGTAGGAGTG+TGG 0.458875 tig0018650:+120591 Msa1368630:intron
AGCAGCTCATGTTCTCGGTC+TGG 0.477055 tig0018650:+118351 Msa1368630:CDS
TTCGAGTTCTATTGCGTCCA+GGG 0.483316 tig0018650:+118188 Msa1368630:five_prime_UTR
CTTGCGCCATTGTACAAAAC+GGG 0.483536 tig0018650:-118285 None:intergenic
TTTCATTTGTTGAACTGTGA+AGG 0.489687 tig0018650:+118223 Msa1368630:five_prime_UTR
TTTCGAGTTCTATTGCGTCC+AGG 0.496144 tig0018650:+118187 Msa1368630:five_prime_UTR
TAACTCACTGCTTAGATGAT+CGG 0.503644 tig0018650:-121307 None:intergenic
TCTTGCGCCATTGTACAAAA+CGG 0.509754 tig0018650:-118286 None:intergenic
GAGAATAGATGTAAGGGGAA+TGG 0.514696 tig0018650:-118420 None:intergenic
TTATTTGGGAGTAGGAGTGT+GGG 0.524936 tig0018650:+120592 Msa1368630:intron
TTATTTGAGAATAGATGTAA+GGG 0.549227 tig0018650:-118426 None:intergenic
TCTGGGAAATTGGCAATGCT+CGG 0.550050 tig0018650:+120933 Msa1368630:three_prime_UTR
TGAAAACCCCAACTGAACCC+TGG 0.556509 tig0018650:-118205 None:intergenic
TTGCGTCCAGGGTTCAGTTG+GGG 0.562869 tig0018650:+118199 Msa1368630:five_prime_UTR
AAATTGGCAATGCTCGGAAG+TGG 0.564571 tig0018650:+120939 Msa1368630:three_prime_UTR
TGAGCCTTTCGCCTCTGTAG+TGG 0.565129 tig0018650:-118511 None:intergenic
ACGAGAAATAAAGGAACTGC+CGG 0.582979 tig0018650:-118319 None:intergenic
TCCAATAAAAGAGCGAGTGT+TGG 0.583283 tig0018650:-121352 None:intergenic
ATTCTTAAACATACAGCCCA+AGG 0.610558 tig0018650:-121186 None:intergenic
GGCGCAAGATCAATACTCAC+CGG 0.610616 tig0018650:+118300 Msa1368630:CDS
GTAGAATATTCACCTAATCA+GGG 0.617357 tig0018650:+121246 Msa1368630:three_prime_UTR
AACAATCTCAGAAGACCTTG+AGG 0.620821 tig0018650:-120987 None:intergenic
GAAAGGCTCACTGAAAGAGA+AGG 0.649077 tig0018650:+118524 Msa1368630:CDS
AAAGACCAAATATAAACTCG+AGG 0.661005 tig0018650:-121026 None:intergenic
CCCAGAAACAACCACTACAG+AGG 0.669217 tig0018650:+118500 Msa1368630:CDS
ACAACCACTACAGAGGCGAA+AGG 0.669954 tig0018650:+118507 Msa1368630:CDS
TATTTGAGAATAGATGTAAG+GGG 0.684162 tig0018650:-118425 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACTCAATATTTTTATTTT+TGG - tig0018650:119447-119466 None:intergenic 10.0%
!!! CTAAAATTGTTATTTATTTA+TGG + tig0018650:120239-120258 Msa1368630:intron 10.0%
!!! GTAATTGTTTTCAATTTTAT+AGG + tig0018650:118583-118602 Msa1368630:intron 15.0%
!!! TTTGATTTTTCAAAATTGAT+AGG + tig0018650:118719-118738 Msa1368630:intron 15.0%
!!! TAGTTTCTATATATGATTTT+GGG - tig0018650:118853-118872 None:intergenic 15.0%
!!! ATAGAAACTAGTAATTTTTT+TGG + tig0018650:118863-118882 Msa1368630:intron 15.0%
!!! AAATATTGAGTTTTTCAATT+TGG + tig0018650:119454-119473 Msa1368630:intron 15.0%
!!! AATATTGAGTTTTTCAATTT+GGG + tig0018650:119455-119474 Msa1368630:intron 15.0%
!! AATATATTTCTCAATAAAGT+TGG + tig0018650:119913-119932 Msa1368630:intron 15.0%
!! TAAATAAAGTAATTTAACCT+TGG + tig0018650:120303-120322 Msa1368630:intron 15.0%
!!! GATAAATTTTTAAGCTTTTA+AGG - tig0018650:120346-120365 None:intergenic 15.0%
!! ACTTCATTATTTGTTTATTT+GGG + tig0018650:120578-120597 Msa1368630:intron 15.0%
!! TTATTTGAGAATAGATGTAA+GGG - tig0018650:118429-118448 None:intergenic 20.0%
!! GATTTGAACTATAATGTAAA+TGG + tig0018650:118627-118646 Msa1368630:intron 20.0%
!!! AGTTTCTATATATGATTTTG+GGG - tig0018650:118852-118871 None:intergenic 20.0%
!!! CTAGTTTCTATATATGATTT+TGG - tig0018650:118854-118873 None:intergenic 20.0%
!! GTATTTGTAAATAAACTAGT+AGG - tig0018650:118935-118954 None:intergenic 20.0%
!! AAAAAAATTCTATTGATGAG+AGG + tig0018650:119280-119299 Msa1368630:intron 20.0%
!! AAAAAATTCTATTGATGAGA+GGG + tig0018650:119281-119300 Msa1368630:intron 20.0%
!!! ATATTGAGTTTTTCAATTTG+GGG + tig0018650:119456-119475 Msa1368630:intron 20.0%
!! AAGTATATAAACCTATCAAT+CGG - tig0018650:119958-119977 None:intergenic 20.0%
!!! TACATTTATTTTATGTCTGT+AGG + tig0018650:119996-120015 Msa1368630:intron 20.0%
!! CACTTCATTATTTGTTTATT+TGG + tig0018650:120577-120596 Msa1368630:intron 20.0%
!!! TATGATACTTGTTAATTTGT+TGG + tig0018650:121159-121178 Msa1368630:three_prime_UTR 20.0%
! ATTAGAAGAACGAGAAATAA+AGG - tig0018650:118331-118350 None:intergenic 25.0%
! TATTTGAGAATAGATGTAAG+GGG - tig0018650:118428-118447 None:intergenic 25.0%
! GTTATTTGAGAATAGATGTA+AGG - tig0018650:118430-118449 None:intergenic 25.0%
! TTATAAATCCAAAATACCTG+CGG - tig0018650:118472-118491 None:intergenic 25.0%
! ACACTGTCTGATATAAATTA+GGG - tig0018650:118565-118584 None:intergenic 25.0%
! TACACTGTCTGATATAAATT+AGG - tig0018650:118566-118585 None:intergenic 25.0%
! GAACTATAATGTAAATGGAT+CGG + tig0018650:118632-118651 Msa1368630:intron 25.0%
! GTTTAAATTCATATGTTGCT+GGG + tig0018650:118661-118680 Msa1368630:intron 25.0%
! TTTAAATTCATATGTTGCTG+GGG + tig0018650:118662-118681 Msa1368630:intron 25.0%
! AAACCTAATAAATCAGAATG+CGG - tig0018650:118787-118806 None:intergenic 25.0%
!!! CTGATTTATTAGGTTTTAAG+AGG + tig0018650:118791-118810 Msa1368630:intron 25.0%
!! GTTTCTATATATGATTTTGG+GGG - tig0018650:118851-118870 None:intergenic 25.0%
!! ATATGACTCAGGTATATTTT+GGG + tig0018650:119231-119250 Msa1368630:intron 25.0%
! ATTCATGGGAGATAAATTTA+TGG + tig0018650:119320-119339 Msa1368630:intron 25.0%
! GATATTGCATATTATATGCA+GGG + tig0018650:119347-119366 Msa1368630:intron 25.0%
! ATATTGCATATTATATGCAG+GGG + tig0018650:119348-119367 Msa1368630:intron 25.0%
!! ACTTTTGAATTGCAAAGAAA+AGG - tig0018650:119505-119524 None:intergenic 25.0%
! ACTTCAATTTAACAACATTG+AGG - tig0018650:119544-119563 None:intergenic 25.0%
! ATGTTGTTAAATTGAAGTCA+TGG + tig0018650:119546-119565 Msa1368630:intron 25.0%
!! CATGGAGATTATTTTCTTTT+AGG - tig0018650:119727-119746 None:intergenic 25.0%
! CTAAAAGAAAATAATCTCCA+TGG + tig0018650:119725-119744 Msa1368630:intron 25.0%
!! TTTAACTTCTAGACAATCTT+TGG - tig0018650:119856-119875 None:intergenic 25.0%
! TAATCTAAACTCCCAAAATA+TGG - tig0018650:120068-120087 None:intergenic 25.0%
! TTATTTGTTTATTTGGGAGT+AGG + tig0018650:120584-120603 Msa1368630:intron 25.0%
! CAGTTGTTAAATTGAAATCT+AGG + tig0018650:120808-120827 Msa1368630:intron 25.0%
!! ACAAAAATTTCTTTTCTCCA+AGG - tig0018650:120903-120922 None:intergenic 25.0%
!!! AATTTTGATCATTTTCCTCA+AGG + tig0018650:120972-120991 Msa1368630:three_prime_UTR 25.0%
!!! CAACTCAAATTTTGTTTTAC+AGG - tig0018650:121094-121113 None:intergenic 25.0%
!!! GTTTTTTGATAGAAGAATAG+TGG + tig0018650:121224-121243 Msa1368630:three_prime_UTR 25.0%
! TTTCATTTGTTGAACTGTGA+AGG + tig0018650:118223-118242 Msa1368630:five_prime_UTR 30.0%
CTGAAAGAAAAAAGAAACAC+TGG + tig0018650:118248-118267 Msa1368630:five_prime_UTR 30.0%
CGTTTAAATTCATATGTTGC+TGG + tig0018650:118660-118679 Msa1368630:intron 30.0%
AACCTAATAAATCAGAATGC+GGG - tig0018650:118786-118805 None:intergenic 30.0%
! GAAAGTAAAGTACTCTTACA+GGG + tig0018650:118885-118904 Msa1368630:intron 30.0%
CATGCAAACCTAAAATGTAA+GGG - tig0018650:119071-119090 None:intergenic 30.0%
AAAATAGCATGATATGACTC+AGG + tig0018650:119220-119239 Msa1368630:intron 30.0%
! GATATGACTCAGGTATATTT+TGG + tig0018650:119230-119249 Msa1368630:intron 30.0%
TCAATTGACTAGTACATTCA+TGG + tig0018650:119305-119324 Msa1368630:intron 30.0%
CAATTGACTAGTACATTCAT+GGG + tig0018650:119306-119325 Msa1368630:intron 30.0%
ATGGGAGATAAATTTATGGT+AGG + tig0018650:119324-119343 Msa1368630:intron 30.0%
TGGGAGATAAATTTATGGTA+GGG + tig0018650:119325-119344 Msa1368630:intron 30.0%
GGATATTGCATATTATATGC+AGG + tig0018650:119346-119365 Msa1368630:intron 30.0%
ACAATTAAATTGTGGAGAAG+TGG - tig0018650:119397-119416 None:intergenic 30.0%
AGAAAAGGAGAATCATCAAT+TGG - tig0018650:119490-119509 None:intergenic 30.0%
CTTTGCAATTCAAAAGTTTG+TGG + tig0018650:119508-119527 Msa1368630:intron 30.0%
TTGTTAATGATCCGATTGAT+AGG + tig0018650:119944-119963 Msa1368630:intron 30.0%
TTATCAATGCACAATTTCCA+AGG - tig0018650:120323-120342 None:intergenic 30.0%
ACGAACGGGTATTAAATAAA+GGG - tig0018650:120445-120464 None:intergenic 30.0%
AATGCATACAATAGACAATG+TGG - tig0018650:120739-120758 None:intergenic 30.0%
!! TTACTTTTGTAGTCCAACTA+GGG + tig0018650:120775-120794 Msa1368630:intron 30.0%
! ATTTTTGTAGCTGATTGTTC+TGG + tig0018650:120915-120934 Msa1368630:exon 30.0%
! TTTTTGTAGCTGATTGTTCT+GGG + tig0018650:120916-120935 Msa1368630:exon 30.0%
TCTATCCTCGAGTTTATATT+TGG + tig0018650:121021-121040 Msa1368630:three_prime_UTR 30.0%
AAAGACCAAATATAAACTCG+AGG - tig0018650:121029-121048 None:intergenic 30.0%
!!! AGTTGATGAAGTGCATTTTT+AGG + tig0018650:121109-121128 Msa1368630:three_prime_UTR 30.0%
!! TTAATTTGTTGGTATGCCTT+GGG + tig0018650:121170-121189 Msa1368630:three_prime_UTR 30.0%
GTAGAATATTCACCTAATCA+GGG + tig0018650:121246-121265 Msa1368630:three_prime_UTR 30.0%
! GCTCTTTTATTGGATGATAT+CGG + tig0018650:121361-121380 Msa1368630:three_prime_UTR 30.0%
AACTACACACGATGAACTAA+AGG - tig0018650:118746-118765 None:intergenic 35.0%
ACCTAATAAATCAGAATGCG+GGG - tig0018650:118785-118804 None:intergenic 35.0%
!!! GGTTTTAAGAGGATCTATGT+AGG + tig0018650:118802-118821 Msa1368630:intron 35.0%
! GGAAAGTAAAGTACTCTTAC+AGG + tig0018650:118884-118903 Msa1368630:intron 35.0%
! ATCGTTTTCGTAGCTCATAT+AGG + tig0018650:118989-119008 Msa1368630:intron 35.0%
!! GAGCATTGAAATTGATGATC+TGG + tig0018650:119030-119049 Msa1368630:intron 35.0%
! TTTAGCGTCCCTTACATTTT+AGG + tig0018650:119060-119079 Msa1368630:intron 35.0%
CCATGCAAACCTAAAATGTA+AGG - tig0018650:119072-119091 None:intergenic 35.0%
!!! CCTTACATTTTAGGTTTGCA+TGG + tig0018650:119069-119088 Msa1368630:intron 35.0%
! AGTACGAGCAAGTTTGAAAT+AGG + tig0018650:119165-119184 Msa1368630:intron 35.0%
GAGCTCACACAATTAAATTG+TGG - tig0018650:119405-119424 None:intergenic 35.0%
!! TTTTTGGTCAAGTAGACTAG+TGG - tig0018650:119431-119450 None:intergenic 35.0%
CAAAAGTTTGTGGTTCCTAT+TGG + tig0018650:119518-119537 Msa1368630:intron 35.0%
TTAACAACATTGAGGCCAAT+AGG - tig0018650:119536-119555 None:intergenic 35.0%
ATTCCTTCACAAACTTCCTA+CGG - tig0018650:119614-119633 None:intergenic 35.0%
!!! GTGTTTATATGGCGGATTTT+CGG + tig0018650:119667-119686 Msa1368630:intron 35.0%
AAAACCAATGTTGTCAACAG+CGG - tig0018650:119704-119723 None:intergenic 35.0%
GCTTTAACAAGATCTGAACT+AGG - tig0018650:119759-119778 None:intergenic 35.0%
ATCCACAACTTCGATAAAGA+TGG - tig0018650:119781-119800 None:intergenic 35.0%
ATATAGTCTGCCTCTACTTA+TGG + tig0018650:119799-119818 Msa1368630:intron 35.0%
CTTGAAAAAGCCATAAGTAG+AGG - tig0018650:119812-119831 None:intergenic 35.0%
AGTGCACTATTAACGTTACA+AGG - tig0018650:120029-120048 None:intergenic 35.0%
CTAAACTCCCAAAATATGGT+GGG - tig0018650:120064-120083 None:intergenic 35.0%
TCTAAACTCCCAAAATATGG+TGG - tig0018650:120065-120084 None:intergenic 35.0%
GTACAATCACCATATTTCCA+AGG - tig0018650:120170-120189 None:intergenic 35.0%
ATCCTTATCTTGTGCCTTAA+GGG + tig0018650:120404-120423 Msa1368630:intron 35.0%
! AATGCTAATTTGTGCCCTTA+AGG - tig0018650:120421-120440 None:intergenic 35.0%
CACGAACGGGTATTAAATAA+AGG - tig0018650:120446-120465 None:intergenic 35.0%
!! CTTACTTTTGTAGTCCAACT+AGG + tig0018650:120774-120793 Msa1368630:intron 35.0%
!! TGATGAAGTGCATTTTTAGG+AGG + tig0018650:121112-121131 Msa1368630:three_prime_UTR 35.0%
!! GTTAATTTGTTGGTATGCCT+TGG + tig0018650:121169-121188 Msa1368630:three_prime_UTR 35.0%
ATTCTTAAACATACAGCCCA+AGG - tig0018650:121189-121208 None:intergenic 35.0%
GGTAGAATATTCACCTAATC+AGG + tig0018650:121245-121264 Msa1368630:three_prime_UTR 35.0%
TAACACATATCACCCTGATT+AGG - tig0018650:121261-121280 None:intergenic 35.0%
TAACTCACTGCTTAGATGAT+CGG - tig0018650:121310-121329 None:intergenic 35.0%
! CTTCTTCCCGTTTTGTACAA+TGG + tig0018650:118279-118298 Msa1368630:exon 40.0%
TCTTGCGCCATTGTACAAAA+CGG - tig0018650:118289-118308 None:intergenic 40.0%
ACGAGAAATAAAGGAACTGC+CGG - tig0018650:118322-118341 None:intergenic 40.0%
CTAATAGCAGCTCATGTTCT+CGG + tig0018650:118346-118365 Msa1368630:CDS 40.0%
TGAAATGAAGTGAGGGAAAG+AGG - tig0018650:118389-118408 None:intergenic 40.0%
GGTAGCATGAAATGAAGTGA+GGG - tig0018650:118396-118415 None:intergenic 40.0%
TGGTAGCATGAAATGAAGTG+AGG - tig0018650:118397-118416 None:intergenic 40.0%
GAGAATAGATGTAAGGGGAA+TGG - tig0018650:118423-118442 None:intergenic 40.0%
CCAAAATACCTGCGGTAAAT+CGG - tig0018650:118464-118483 None:intergenic 40.0%
ACCCCGCATTCTGATTTATT+AGG + tig0018650:118781-118800 Msa1368630:intron 40.0%
AATGTAAGGGACGCTAAAGA+TGG - tig0018650:119058-119077 None:intergenic 40.0%
GTAGGAAGTTTGTGAAGGAA+TGG + tig0018650:119613-119632 Msa1368630:intron 40.0%
TCCGCAATGGTGTGTTTATA+TGG + tig0018650:119656-119675 Msa1368630:intron 40.0%
GCCATATAAACACACCATTG+CGG - tig0018650:119660-119679 None:intergenic 40.0%
GCAATGGTGTGTTTATATGG+CGG + tig0018650:119659-119678 Msa1368630:intron 40.0%
! TGAACTAGGTTGAGTATCCA+TGG - tig0018650:119745-119764 None:intergenic 40.0%
AGCCATCTTTATCGAAGTTG+TGG + tig0018650:119776-119795 Msa1368630:intron 40.0%
!!! GGCTTTTTCAAGCAACATGT+TGG + tig0018650:119820-119839 Msa1368630:intron 40.0%
! TCCATTGCCCACCATATTTT+GGG + tig0018650:120054-120073 Msa1368630:intron 40.0%
TTGAGTCAGCCTTGGAAATA+TGG + tig0018650:120158-120177 Msa1368630:intron 40.0%
TTCTTATCCTTGTGCCCTTA+GGG - tig0018650:120281-120300 None:intergenic 40.0%
TTTCTTATCCTTGTGCCCTT+AGG - tig0018650:120282-120301 None:intergenic 40.0%
GATCCTTATCTTGTGCCTTA+AGG + tig0018650:120403-120422 Msa1368630:intron 40.0%
AACTGCATTAAACAGCACAC+AGG - tig0018650:120472-120491 None:intergenic 40.0%
ATGCAGTTTGTGCCAAACTA+AGG + tig0018650:120484-120503 Msa1368630:intron 40.0%
CTCAAGTTAGAGCCTTAGTT+TGG - tig0018650:120499-120518 None:intergenic 40.0%
TTTATTTGGGAGTAGGAGTG+TGG + tig0018650:120591-120610 Msa1368630:intron 40.0%
! TTATTTGGGAGTAGGAGTGT+GGG + tig0018650:120592-120611 Msa1368630:intron 40.0%
! GGTCTGGAGTTAACTAAAGA+TGG - tig0018650:120707-120726 None:intergenic 40.0%
TAGACAATGTGGACTTAGAC+TGG - tig0018650:120728-120747 None:intergenic 40.0%
CAGTTACAAGTTTGCATCCT+TGG + tig0018650:120883-120902 Msa1368630:intron 40.0%
!! AGCTGATTGTTCTGGGAAAT+TGG + tig0018650:120923-120942 Msa1368630:exon 40.0%
AACAATCTCAGAAGACCTTG+AGG - tig0018650:120990-121009 None:intergenic 40.0%
! TGTTTTACAGGAAGAACGAC+TGG - tig0018650:121082-121101 None:intergenic 40.0%
! ACCAACACTCGCTCTTTTAT+TGG + tig0018650:121351-121370 Msa1368630:three_prime_UTR 40.0%
TCCAATAAAAGAGCGAGTGT+TGG - tig0018650:121355-121374 None:intergenic 40.0%
!! TTTCGAGTTCTATTGCGTCC+AGG + tig0018650:118187-118206 Msa1368630:five_prime_UTR 45.0%
! TTCGAGTTCTATTGCGTCCA+GGG + tig0018650:118188-118207 Msa1368630:five_prime_UTR 45.0%
CTTGCGCCATTGTACAAAAC+GGG - tig0018650:118288-118307 None:intergenic 45.0%
AGATGTAAGGGGAATGGAAC+TGG - tig0018650:118417-118436 None:intergenic 45.0%
! CCGATTTACCGCAGGTATTT+TGG + tig0018650:118461-118480 Msa1368630:intron 45.0%
!! CCTCTGTAGTGGTTGTTTCT+GGG - tig0018650:118503-118522 None:intergenic 45.0%
GAAAGGCTCACTGAAAGAGA+AGG + tig0018650:118524-118543 Msa1368630:CDS 45.0%
TCTTACAGGGCTCATCAGTT+TGG + tig0018650:118898-118917 Msa1368630:intron 45.0%
TGCATATTATATGCAGGGGC+CGG + tig0018650:119352-119371 Msa1368630:intron 45.0%
!! GGAATGCCACGATGGATTTT+TGG + tig0018650:119581-119600 Msa1368630:intron 45.0%
GGTTGTCCAAAAATCCATCG+TGG - tig0018650:119590-119609 None:intergenic 45.0%
!! GATTTTTGGACAACCACCGT+AGG + tig0018650:119595-119614 Msa1368630:intron 45.0%
! GTCCATTGCCCACCATATTT+TGG + tig0018650:120053-120072 Msa1368630:intron 45.0%
!! TCCCAAAATATGGTGGGCAA+TGG - tig0018650:120058-120077 None:intergenic 45.0%
GCTCGAAAAATGCACACAAC+AGG - tig0018650:120134-120153 None:intergenic 45.0%
! TTTCCAAGGCTGACTCAAGA+AGG - tig0018650:120156-120175 None:intergenic 45.0%
TCTTGCTAACTTCTGCCCTA+AGG + tig0018650:120263-120282 Msa1368630:intron 45.0%
CTTGCTAACTTCTGCCCTAA+GGG + tig0018650:120264-120283 Msa1368630:intron 45.0%
TGCCCTTAAGGCACAAGATA+AGG - tig0018650:120409-120428 None:intergenic 45.0%
!! TAATGAAGTGCTGCGAGTGA+TGG - tig0018650:120567-120586 None:intergenic 45.0%
! AATGTGGACTTAGACTGGTC+TGG - tig0018650:120723-120742 None:intergenic 45.0%
TCTGGGAAATTGGCAATGCT+CGG + tig0018650:120933-120952 Msa1368630:three_prime_UTR 45.0%
AAATTGGCAATGCTCGGAAG+TGG + tig0018650:120939-120958 Msa1368630:three_prime_UTR 45.0%
!!! TAAAATTGTTATTTATTTAT+GGG + tig0018650:120240-120259 Msa1368630:intron 5.0%
TATTGCGTCCAGGGTTCAGT+TGG + tig0018650:118197-118216 Msa1368630:five_prime_UTR 50.0%
ATTGCGTCCAGGGTTCAGTT+GGG + tig0018650:118198-118217 Msa1368630:five_prime_UTR 50.0%
TGAAAACCCCAACTGAACCC+TGG - tig0018650:118208-118227 None:intergenic 50.0%
GGCGCAAGATCAATACTCAC+CGG + tig0018650:118300-118319 Msa1368630:CDS 50.0%
! GTGCTTTTCCGATTTACCGC+AGG + tig0018650:118453-118472 Msa1368630:CDS 50.0%
CCCAGAAACAACCACTACAG+AGG + tig0018650:118500-118519 Msa1368630:CDS 50.0%
! GCCTCTGTAGTGGTTGTTTC+TGG - tig0018650:118504-118523 None:intergenic 50.0%
ACAACCACTACAGAGGCGAA+AGG + tig0018650:118507-118526 Msa1368630:CDS 50.0%
GGCTCACTGAAAGAGAAGGT+TGG + tig0018650:118528-118547 Msa1368630:intron 50.0%
GGGTGCGCAATTCTCTAATC+TGG + tig0018650:119251-119270 Msa1368630:intron 50.0%
GCATATTATATGCAGGGGCC+GGG + tig0018650:119353-119372 Msa1368630:intron 50.0%
CATATTATATGCAGGGGCCG+GGG + tig0018650:119354-119373 Msa1368630:intron 50.0%
! TTGTGGAGAAGTGGAGTGTC+CGG - tig0018650:119388-119407 None:intergenic 50.0%
CCTTCACAAACTTCCTACGG+TGG - tig0018650:119611-119630 None:intergenic 50.0%
CCACCGTAGGAAGTTTGTGA+AGG + tig0018650:119608-119627 Msa1368630:intron 50.0%
TTGTGAAGGAATGGCTGCCA+TGG + tig0018650:119622-119641 Msa1368630:intron 50.0%
ATGTTGTCAACAGCGGATGG+CGG - tig0018650:119697-119716 None:intergenic 50.0%
CCAATGTTGTCAACAGCGGA+TGG - tig0018650:119700-119719 None:intergenic 50.0%
CCATCCGCTGTTGACAACAT+TGG + tig0018650:119697-119716 Msa1368630:intron 50.0%
! GAGTAGGAGTGTGGGTCTTA+TGG + tig0018650:120600-120619 Msa1368630:intron 50.0%
CTGATTAGTGCTGCCCTAGT+TGG - tig0018650:120791-120810 None:intergenic 50.0%
TTGCGTCCAGGGTTCAGTTG+GGG + tig0018650:118199-118218 Msa1368630:five_prime_UTR 55.0%
AGCAGCTCATGTTCTCGGTC+TGG + tig0018650:118351-118370 Msa1368630:CDS 55.0%
TGAGCCTTTCGCCTCTGTAG+TGG - tig0018650:118514-118533 None:intergenic 55.0%
! TGTGGAGAAGTGGAGTGTCC+GGG - tig0018650:119387-119406 None:intergenic 55.0%
!! TTGAAGTCATGGTGCCGCCA+TGG + tig0018650:119557-119576 Msa1368630:intron 55.0%
ATCGTGGCATTCCACCATGG+CGG - tig0018650:119574-119593 None:intergenic 55.0%
TCCATCGTGGCATTCCACCA+TGG - tig0018650:119577-119596 None:intergenic 55.0%
AAGGAATGGCTGCCATGGCA+GGG + tig0018650:119627-119646 Msa1368630:intron 55.0%
GAGCCTTCTTGAGTCAGCCT+TGG + tig0018650:120150-120169 Msa1368630:intron 55.0%
! ACTTCTGCCCTAAGGGCACA+AGG + tig0018650:120271-120290 Msa1368630:intron 55.0%
AGCACACAGGATGCACGAAC+GGG - tig0018650:120459-120478 None:intergenic 55.0%
CAGCACACAGGATGCACGAA+CGG - tig0018650:120460-120479 None:intergenic 55.0%
! GTGGAGAAGTGGAGTGTCCG+GGG - tig0018650:119386-119405 None:intergenic 60.0%
!! AAGTCATGGTGCCGCCATGG+TGG + tig0018650:119560-119579 Msa1368630:intron 60.0%
! GCCATGGTGGAATGCCACGA+TGG + tig0018650:119573-119592 Msa1368630:intron 60.0%
GAAGGAATGGCTGCCATGGC+AGG + tig0018650:119626-119645 Msa1368630:intron 60.0%
!! TGTCAACAGCGGATGGCGGA+AGG - tig0018650:119693-119712 None:intergenic 60.0%
! TGCGGACTATCGCCCTGCCA+TGG - tig0018650:119642-119661 None:intergenic 65.0%
GGCAGGGCGATAGTCCGCAA+TGG + tig0018650:119643-119662 Msa1368630:intron 65.0%
AGTGTCCGGGGTTCGAGCCC+CGG - tig0018650:119374-119393 None:intergenic 70.0%
! AGGGGCCGGGGCTCGAACCC+CGG + tig0018650:119366-119385 Msa1368630:intron 80.0%
Chromosome Type Strat End Strand Name
tig0018650 gene 118183 121431 118183 ID=Msa1368630;Name=Msa1368630
tig0018650 mRNA 118183 121431 118183 ID=Msa1368630-mRNA-1;Parent=Msa1368630;Name=Msa1368630-mRNA-1;_AED=0.13;_eAED=0.14;_QI=115|0|0.4|1|0|0.2|5|507|75
tig0018650 exon 118183 118372 118183 ID=Msa1368630-mRNA-1:exon:582;Parent=Msa1368630-mRNA-1
tig0018650 exon 118418 118474 118418 ID=Msa1368630-mRNA-1:exon:583;Parent=Msa1368630-mRNA-1
tig0018650 exon 118497 118545 118497 ID=Msa1368630-mRNA-1:exon:584;Parent=Msa1368630-mRNA-1
tig0018650 exon 120606 120613 120606 ID=Msa1368630-mRNA-1:exon:585;Parent=Msa1368630-mRNA-1
tig0018650 exon 120886 121431 120886 ID=Msa1368630-mRNA-1:exon:586;Parent=Msa1368630-mRNA-1
tig0018650 five_prime_UTR 118183 118297 118183 ID=Msa1368630-mRNA-1:five_prime_utr;Parent=Msa1368630-mRNA-1
tig0018650 CDS 118298 118372 118298 ID=Msa1368630-mRNA-1:cds;Parent=Msa1368630-mRNA-1
tig0018650 CDS 118418 118474 118418 ID=Msa1368630-mRNA-1:cds;Parent=Msa1368630-mRNA-1
tig0018650 CDS 118497 118545 118497 ID=Msa1368630-mRNA-1:cds;Parent=Msa1368630-mRNA-1
tig0018650 CDS 120606 120613 120606 ID=Msa1368630-mRNA-1:cds;Parent=Msa1368630-mRNA-1
tig0018650 CDS 120886 120924 120886 ID=Msa1368630-mRNA-1:cds;Parent=Msa1368630-mRNA-1
tig0018650 three_prime_UTR 120925 121431 120925 ID=Msa1368630-mRNA-1:three_prime_utr;Parent=Msa1368630-mRNA-1
Gene Sequence

>Msa1368630

ATGGCGCAAGATCAATACTCACCGGCAGTTCCTTTATTTCTCGTTCTTCTAATAGCAGCTCATGTTCTCGGTCTGTTCCATTCCCCTTACATCTATTCTCAAATAACTGTGTGCTTTTCCGATTTACCGCAGCTACCCAGAAACAACCACTACAGAGGCGAAAGGCTCACTGAAAGAGAAGGAGTGTGGTTACAAGTTTGCATCCTTGGAGAAAAGAAATTTTTGTAG
Protein sequence

>Msa1368630

MAQDQYSPAVPLFLVLLIAAHVLGLFHSPYIYSQITVCFSDLPQLPRNNHYRGERLTEREGVWLQVCILGEKKFL*