AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0431920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0431920 MtrunA17_Chr3g0136731 84.615 52 1 1 1 45 1 52 5.03e-24 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATCCTCGAGTTTATATT+TGG 0.181930 3_2:+84046890 Msa0431920:three_prime_UTR
CCGATTTACCGCAGGTATTT+TGG 0.194402 3_2:+84044333 Msa0431920:intron
GGTAGAATATTCACCTAATC+AGG 0.266410 3_2:+84047114 Msa0431920:three_prime_UTR
AGCTGATTGTTCTGGGAAAT+TGG 0.293695 3_2:+84046792 Msa0431920:three_prime_UTR
CAGTTACAAGTTTGCATCCT+TGG 0.331082 3_2:+84046752 Msa0431920:intron
GTTAATTTGTTGGTATGCCT+TGG 0.359869 3_2:+84047038 Msa0431920:three_prime_UTR
TATGATACTTGTTAATTTGT+TGG 0.367418 3_2:+84047028 Msa0431920:three_prime_UTR
ATTAGAAGAACGAGAAATAA+AGG 0.372863 3_2:-84044199 None:intergenic
CTAATAGCAGCTCATGTTCT+CGG 0.382026 3_2:+84044217 Msa0431920:CDS
CAACTCTTATCACTTTATAT+AGG 0.387456 3_2:-84047281 None:intergenic
TTAATTTGTTGGTATGCCTT+GGG 0.390776 3_2:+84047039 Msa0431920:three_prime_UTR
ATTGCGTCCAGGGTTCAGTT+GGG 0.433906 3_2:+84044069 Msa0431920:five_prime_UTR
TATTGCGTCCAGGGTTCAGT+TGG 0.448218 3_2:+84044068 Msa0431920:five_prime_UTR
TAACACATATCACCCTGATT+AGG 0.452705 3_2:-84047127 None:intergenic
AGCAGCTCATGTTCTCGGTC+TGG 0.477055 3_2:+84044222 Msa0431920:CDS
TTCGAGTTCTATTGCGTCCA+GGG 0.483316 3_2:+84044059 Msa0431920:five_prime_UTR
CTTGCGCCATTGTACAAAAC+GGG 0.483536 3_2:-84044156 None:intergenic
TTTCATTTGTTGAACTGTGA+AGG 0.489687 3_2:+84044094 Msa0431920:five_prime_UTR
TTTCGAGTTCTATTGCGTCC+AGG 0.496144 3_2:+84044058 Msa0431920:five_prime_UTR
TAACTCACTGCTTAGATGAT+CGG 0.503644 3_2:-84047176 None:intergenic
TCTTGCGCCATTGTACAAAA+CGG 0.509754 3_2:-84044157 None:intergenic
TCTGGGAAATTGGCAATGCT+CGG 0.550050 3_2:+84046802 Msa0431920:three_prime_UTR
TGAAAACCCCAACTGAACCC+TGG 0.556509 3_2:-84044076 None:intergenic
TTGCGTCCAGGGTTCAGTTG+GGG 0.562869 3_2:+84044070 Msa0431920:five_prime_UTR
AAATTGGCAATGCTCGGAAG+TGG 0.564571 3_2:+84046808 Msa0431920:three_prime_UTR
TGAGCCTTTCGCCTCTGCAG+TGG 0.573093 3_2:-84044383 None:intergenic
ACGAGAAATAAAGGAACTGC+CGG 0.582979 3_2:-84044190 None:intergenic
TCCAATAAAAGAGCGAGTGT+TGG 0.583283 3_2:-84047221 None:intergenic
ATTCTTAAACATACAGCCCA+AGG 0.610558 3_2:-84047055 None:intergenic
GGCGCAAGATCAATACTCAC+CGG 0.610616 3_2:+84044171 Msa0431920:CDS
GTAGAATATTCACCTAATCA+GGG 0.617357 3_2:+84047115 Msa0431920:three_prime_UTR
AACAATCTCAGAAGACCTTG+AGG 0.620821 3_2:-84046856 None:intergenic
ACAACCACTGCAGAGGCGAA+AGG 0.629999 3_2:+84044379 Msa0431920:CDS
CTCAGAAACAACCACTGCAG+AGG 0.630477 3_2:+84044372 Msa0431920:CDS
GAAAGGCTCACTGAAAGAGA+AGG 0.649077 3_2:+84044396 Msa0431920:exon
AAAGACCAAATATAAACTCG+AGG 0.661005 3_2:-84046895 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACTCAATATTTTTATTTT+TGG - chr3_2:84045316-84045335 None:intergenic 10.0%
!!! CTAAAATTGTTATTTATTTA+TGG + chr3_2:84046108-84046127 Msa0431920:intron 10.0%
!!! GTAATTGTTTTCAATTTTAT+AGG + chr3_2:84044455-84044474 Msa0431920:intron 15.0%
!!! TTAGATTTTTCAAAATTGAT+AGG + chr3_2:84044589-84044608 Msa0431920:intron 15.0%
!!! TAGTTTCTATATATGATTTT+GGG - chr3_2:84044723-84044742 None:intergenic 15.0%
!!! ATAGAAACTAGTAATTTTTT+TGG + chr3_2:84044733-84044752 Msa0431920:intron 15.0%
!!! AAATATTGAGTTTTTCAATT+TGG + chr3_2:84045323-84045342 Msa0431920:intron 15.0%
!!! AATATTGAGTTTTTCAATTT+GGG + chr3_2:84045324-84045343 Msa0431920:intron 15.0%
!! AATATATTTCTCAATAAAGT+TGG + chr3_2:84045782-84045801 Msa0431920:intron 15.0%
!! TAAATAAAGTAATTTAACCT+TGG + chr3_2:84046172-84046191 Msa0431920:intron 15.0%
!!! GATAAATTTTTAAGCTTTTA+AGG - chr3_2:84046215-84046234 None:intergenic 15.0%
!! ACTTCATTATTTGTTTATTT+GGG + chr3_2:84046447-84046466 Msa0431920:intron 15.0%
!! TTATTTGAGAATAGATGTAA+GGG - chr3_2:84044301-84044320 None:intergenic 20.0%
!! ACACTGTCTAATATAAATTA+GGG - chr3_2:84044437-84044456 None:intergenic 20.0%
!! TACACTGTCTAATATAAATT+AGG - chr3_2:84044438-84044457 None:intergenic 20.0%
!!! AGTTTCTATATATGATTTTG+GGG - chr3_2:84044722-84044741 None:intergenic 20.0%
!!! CTAGTTTCTATATATGATTT+TGG - chr3_2:84044724-84044743 None:intergenic 20.0%
!! GTATTTGTAAATAAACTAGT+AGG - chr3_2:84044804-84044823 None:intergenic 20.0%
!! AAAAAAATTCTATTGATGAG+AGG + chr3_2:84045149-84045168 Msa0431920:intron 20.0%
!! AAAAAATTCTATTGATGAGA+GGG + chr3_2:84045150-84045169 Msa0431920:intron 20.0%
!!! ATATTGAGTTTTTCAATTTG+GGG + chr3_2:84045325-84045344 Msa0431920:intron 20.0%
!! AAGTATATAAACCTATCAAT+CGG - chr3_2:84045827-84045846 None:intergenic 20.0%
!!! TACATTTATTTTATGTCTGT+AGG + chr3_2:84045865-84045884 Msa0431920:intron 20.0%
!! CACTTCATTATTTGTTTATT+TGG + chr3_2:84046446-84046465 Msa0431920:intron 20.0%
!!! TATGATACTTGTTAATTTGT+TGG + chr3_2:84047028-84047047 Msa0431920:three_prime_UTR 20.0%
! ATTAGAAGAACGAGAAATAA+AGG - chr3_2:84044202-84044221 None:intergenic 25.0%
! TATTTGAGAATAGATGTAAG+GGG - chr3_2:84044300-84044319 None:intergenic 25.0%
! GTTATTTGAGAATAGATGTA+AGG - chr3_2:84044302-84044321 None:intergenic 25.0%
! TTATAAATCCAAAATACCTG+CGG - chr3_2:84044344-84044363 None:intergenic 25.0%
! TTGAACTAATGTAAATGGAT+TGG + chr3_2:84044502-84044521 Msa0431920:intron 25.0%
! GTTTAAATTCATATGTTGCT+GGG + chr3_2:84044531-84044550 Msa0431920:intron 25.0%
! TTTAAATTCATATGTTGCTG+GGG + chr3_2:84044532-84044551 Msa0431920:intron 25.0%
! AAACCTAATAAATCAGAATG+CGG - chr3_2:84044657-84044676 None:intergenic 25.0%
!!! CTGATTTATTAGGTTTTAAG+AGG + chr3_2:84044661-84044680 Msa0431920:intron 25.0%
!! GTTTCTATATATGATTTTGG+GGG - chr3_2:84044721-84044740 None:intergenic 25.0%
!! ATATGACTCAGGTATATTTT+GGG + chr3_2:84045100-84045119 Msa0431920:intron 25.0%
! ATTCATGGGAGATAAATTTA+TGG + chr3_2:84045189-84045208 Msa0431920:intron 25.0%
! GATATTGCATATTATATGCA+GGG + chr3_2:84045216-84045235 Msa0431920:intron 25.0%
! ATATTGCATATTATATGCAG+GGG + chr3_2:84045217-84045236 Msa0431920:intron 25.0%
!! ACTTTTGAATTGCAAAGAAA+AGG - chr3_2:84045374-84045393 None:intergenic 25.0%
! ACTTCAATTTAACAACATTG+AGG - chr3_2:84045413-84045432 None:intergenic 25.0%
! ATGTTGTTAAATTGAAGTCA+TGG + chr3_2:84045415-84045434 Msa0431920:intron 25.0%
!! CATGGAGATTATTTTCTTTT+AGG - chr3_2:84045596-84045615 None:intergenic 25.0%
! CTAAAAGAAAATAATCTCCA+TGG + chr3_2:84045594-84045613 Msa0431920:intron 25.0%
!! TTTAACTTCTAGACAATCTT+TGG - chr3_2:84045725-84045744 None:intergenic 25.0%
! TAATCTAAACTCCCAAAATA+TGG - chr3_2:84045937-84045956 None:intergenic 25.0%
! TTATTTGTTTATTTGGGAGT+AGG + chr3_2:84046453-84046472 Msa0431920:intron 25.0%
! CAGTTGTTAAATTGAAATCT+AGG + chr3_2:84046677-84046696 Msa0431920:intron 25.0%
!! ACAAAAATTTCTTTTCTCCA+AGG - chr3_2:84046772-84046791 None:intergenic 25.0%
!!! AATTTTGATCATTTTCCTCA+AGG + chr3_2:84046841-84046860 Msa0431920:three_prime_UTR 25.0%
!!! CAACTCAAATTTTGTTTTAC+AGG - chr3_2:84046963-84046982 None:intergenic 25.0%
!!! GTTTTTTGATAGAAGAATAG+TGG + chr3_2:84047093-84047112 Msa0431920:three_prime_UTR 25.0%
! TTTCATTTGTTGAACTGTGA+AGG + chr3_2:84044094-84044113 Msa0431920:five_prime_UTR 30.0%
CTGAAAGAAAAAAGAAACAC+TGG + chr3_2:84044119-84044138 Msa0431920:five_prime_UTR 30.0%
ATTTGAGAATAGATGTAAGG+GGG - chr3_2:84044299-84044318 None:intergenic 30.0%
GCGATTTGAACTAATGTAAA+TGG + chr3_2:84044497-84044516 Msa0431920:intron 30.0%
CGTTTAAATTCATATGTTGC+TGG + chr3_2:84044530-84044549 Msa0431920:intron 30.0%
AACCTAATAAATCAGAATGC+GGG - chr3_2:84044656-84044675 None:intergenic 30.0%
! GAAAGTAAAGTACTCTTACA+GGG + chr3_2:84044755-84044774 Msa0431920:intron 30.0%
CATGCAAACCTAAAATGTAA+GGG - chr3_2:84044940-84044959 None:intergenic 30.0%
AAAATAGCATGATATGACTC+AGG + chr3_2:84045089-84045108 Msa0431920:intron 30.0%
! GATATGACTCAGGTATATTT+TGG + chr3_2:84045099-84045118 Msa0431920:intron 30.0%
TCAATTGACTAGTACATTCA+TGG + chr3_2:84045174-84045193 Msa0431920:intron 30.0%
CAATTGACTAGTACATTCAT+GGG + chr3_2:84045175-84045194 Msa0431920:intron 30.0%
ATGGGAGATAAATTTATGGT+AGG + chr3_2:84045193-84045212 Msa0431920:intron 30.0%
TGGGAGATAAATTTATGGTA+GGG + chr3_2:84045194-84045213 Msa0431920:intron 30.0%
GGATATTGCATATTATATGC+AGG + chr3_2:84045215-84045234 Msa0431920:intron 30.0%
ACAATTAAATTGTGGAGAAG+TGG - chr3_2:84045266-84045285 None:intergenic 30.0%
AGAAAAGGAGAATCATCAAT+TGG - chr3_2:84045359-84045378 None:intergenic 30.0%
CTTTGCAATTCAAAAGTTTG+TGG + chr3_2:84045377-84045396 Msa0431920:intron 30.0%
TTGTTAATGATCCGATTGAT+AGG + chr3_2:84045813-84045832 Msa0431920:intron 30.0%
TTATCAATGCACAATTTCCA+AGG - chr3_2:84046192-84046211 None:intergenic 30.0%
ACGAACGGGTATTAAATAAA+GGG - chr3_2:84046314-84046333 None:intergenic 30.0%
AATGCATACAATAGACAATG+TGG - chr3_2:84046608-84046627 None:intergenic 30.0%
!! TTACTTTTGTAGTCCAACTA+GGG + chr3_2:84046644-84046663 Msa0431920:intron 30.0%
! ATTTTTGTAGCTGATTGTTC+TGG + chr3_2:84046784-84046803 Msa0431920:three_prime_UTR 30.0%
! TTTTTGTAGCTGATTGTTCT+GGG + chr3_2:84046785-84046804 Msa0431920:three_prime_UTR 30.0%
TCTATCCTCGAGTTTATATT+TGG + chr3_2:84046890-84046909 Msa0431920:three_prime_UTR 30.0%
AAAGACCAAATATAAACTCG+AGG - chr3_2:84046898-84046917 None:intergenic 30.0%
!!! AGTTGATGAAGTGCATTTTT+AGG + chr3_2:84046978-84046997 Msa0431920:three_prime_UTR 30.0%
!! TTAATTTGTTGGTATGCCTT+GGG + chr3_2:84047039-84047058 Msa0431920:three_prime_UTR 30.0%
GTAGAATATTCACCTAATCA+GGG + chr3_2:84047115-84047134 Msa0431920:three_prime_UTR 30.0%
! GCTCTTTTATTGGATGATAT+CGG + chr3_2:84047230-84047249 Msa0431920:three_prime_UTR 30.0%
ACCTAATAAATCAGAATGCG+GGG - chr3_2:84044655-84044674 None:intergenic 35.0%
!!! GGTTTTAAGAGGATCTATGT+AGG + chr3_2:84044672-84044691 Msa0431920:intron 35.0%
! GGAAAGTAAAGTACTCTTAC+AGG + chr3_2:84044754-84044773 Msa0431920:intron 35.0%
! ATCGTTTTCGTAGCTCATAT+AGG + chr3_2:84044858-84044877 Msa0431920:intron 35.0%
!! GAGCATTGAAATTGATGATC+TGG + chr3_2:84044899-84044918 Msa0431920:intron 35.0%
! TTTAGCGTCCCTTACATTTT+AGG + chr3_2:84044929-84044948 Msa0431920:intron 35.0%
CCATGCAAACCTAAAATGTA+AGG - chr3_2:84044941-84044960 None:intergenic 35.0%
!!! CCTTACATTTTAGGTTTGCA+TGG + chr3_2:84044938-84044957 Msa0431920:intron 35.0%
! AGTACGAGCAAGTTTGAAAT+AGG + chr3_2:84045034-84045053 Msa0431920:intron 35.0%
GAGCTCACACAATTAAATTG+TGG - chr3_2:84045274-84045293 None:intergenic 35.0%
!! TTTTTGGTCAAGTAGACTAG+TGG - chr3_2:84045300-84045319 None:intergenic 35.0%
CAAAAGTTTGTGGTTCCTAT+TGG + chr3_2:84045387-84045406 Msa0431920:intron 35.0%
TTAACAACATTGAGGCCAAT+AGG - chr3_2:84045405-84045424 None:intergenic 35.0%
ATTCCTTCACAAACTTCCTA+CGG - chr3_2:84045483-84045502 None:intergenic 35.0%
!!! GTGTTTATATGGCGGATTTT+CGG + chr3_2:84045536-84045555 Msa0431920:intron 35.0%
AAAACCAATGTTGTCAACAG+CGG - chr3_2:84045573-84045592 None:intergenic 35.0%
GCTTTAACAAGATCTGAACT+AGG - chr3_2:84045628-84045647 None:intergenic 35.0%
ATCCACAACTTCGATAAAGA+TGG - chr3_2:84045650-84045669 None:intergenic 35.0%
ATATAGTCTGCCTCTACTTA+TGG + chr3_2:84045668-84045687 Msa0431920:intron 35.0%
CTTGAAAAAGCCATAAGTAG+AGG - chr3_2:84045681-84045700 None:intergenic 35.0%
AGTGCACTATTAACGTTACA+AGG - chr3_2:84045898-84045917 None:intergenic 35.0%
CTAAACTCCCAAAATATGGT+GGG - chr3_2:84045933-84045952 None:intergenic 35.0%
TCTAAACTCCCAAAATATGG+TGG - chr3_2:84045934-84045953 None:intergenic 35.0%
GTACAATCACCATATTTCCA+AGG - chr3_2:84046039-84046058 None:intergenic 35.0%
ATCCTTATCTTGTGCCTTAA+GGG + chr3_2:84046273-84046292 Msa0431920:intron 35.0%
! AATGCTAATTTGTGCCCTTA+AGG - chr3_2:84046290-84046309 None:intergenic 35.0%
CACGAACGGGTATTAAATAA+AGG - chr3_2:84046315-84046334 None:intergenic 35.0%
!! CTTACTTTTGTAGTCCAACT+AGG + chr3_2:84046643-84046662 Msa0431920:intron 35.0%
!! TGATGAAGTGCATTTTTAGG+AGG + chr3_2:84046981-84047000 Msa0431920:three_prime_UTR 35.0%
!! GTTAATTTGTTGGTATGCCT+TGG + chr3_2:84047038-84047057 Msa0431920:three_prime_UTR 35.0%
ATTCTTAAACATACAGCCCA+AGG - chr3_2:84047058-84047077 None:intergenic 35.0%
GGTAGAATATTCACCTAATC+AGG + chr3_2:84047114-84047133 Msa0431920:three_prime_UTR 35.0%
TAACACATATCACCCTGATT+AGG - chr3_2:84047130-84047149 None:intergenic 35.0%
TAACTCACTGCTTAGATGAT+CGG - chr3_2:84047179-84047198 None:intergenic 35.0%
! CTTCTTCCCGTTTTGTACAA+TGG + chr3_2:84044150-84044169 Msa0431920:exon 40.0%
TCTTGCGCCATTGTACAAAA+CGG - chr3_2:84044160-84044179 None:intergenic 40.0%
ACGAGAAATAAAGGAACTGC+CGG - chr3_2:84044193-84044212 None:intergenic 40.0%
CTAATAGCAGCTCATGTTCT+CGG + chr3_2:84044217-84044236 Msa0431920:CDS 40.0%
TGAAATGAAGTGAGGGAAAG+AGG - chr3_2:84044260-84044279 None:intergenic 40.0%
GGTAGCATGAAATGAAGTGA+GGG - chr3_2:84044267-84044286 None:intergenic 40.0%
TGGTAGCATGAAATGAAGTG+AGG - chr3_2:84044268-84044287 None:intergenic 40.0%
AGAATAGATGTAAGGGGGAA+TGG - chr3_2:84044294-84044313 None:intergenic 40.0%
CCAAAATACCTGCGGTAAAT+CGG - chr3_2:84044336-84044355 None:intergenic 40.0%
ACTGAAACTACACACGATGA+AGG - chr3_2:84044621-84044640 None:intergenic 40.0%
ACCCCGCATTCTGATTTATT+AGG + chr3_2:84044651-84044670 Msa0431920:intron 40.0%
AATGTAAGGGACGCTAAAGA+TGG - chr3_2:84044927-84044946 None:intergenic 40.0%
GTAGGAAGTTTGTGAAGGAA+TGG + chr3_2:84045482-84045501 Msa0431920:intron 40.0%
TCCGCAATGGTGTGTTTATA+TGG + chr3_2:84045525-84045544 Msa0431920:intron 40.0%
GCCATATAAACACACCATTG+CGG - chr3_2:84045529-84045548 None:intergenic 40.0%
GCAATGGTGTGTTTATATGG+CGG + chr3_2:84045528-84045547 Msa0431920:intron 40.0%
! TGAACTAGGTTGAGTATCCA+TGG - chr3_2:84045614-84045633 None:intergenic 40.0%
AGCCATCTTTATCGAAGTTG+TGG + chr3_2:84045645-84045664 Msa0431920:intron 40.0%
!!! GGCTTTTTCAAGCAACATGT+TGG + chr3_2:84045689-84045708 Msa0431920:intron 40.0%
! TCCATTGCCCACCATATTTT+GGG + chr3_2:84045923-84045942 Msa0431920:intron 40.0%
TTGAGTCAGCCTTGGAAATA+TGG + chr3_2:84046027-84046046 Msa0431920:intron 40.0%
TTCTTATCCTTGTGCCCTTA+GGG - chr3_2:84046150-84046169 None:intergenic 40.0%
TTTCTTATCCTTGTGCCCTT+AGG - chr3_2:84046151-84046170 None:intergenic 40.0%
GATCCTTATCTTGTGCCTTA+AGG + chr3_2:84046272-84046291 Msa0431920:intron 40.0%
AACTGCATTAAACAGCACAC+AGG - chr3_2:84046341-84046360 None:intergenic 40.0%
ATGCAGTTTGTGCCAAACTA+AGG + chr3_2:84046353-84046372 Msa0431920:intron 40.0%
CTCAAGTTAGAGCCTTAGTT+TGG - chr3_2:84046368-84046387 None:intergenic 40.0%
TTTATTTGGGAGTAGGAGTG+TGG + chr3_2:84046460-84046479 Msa0431920:intron 40.0%
! TTATTTGGGAGTAGGAGTGT+GGG + chr3_2:84046461-84046480 Msa0431920:intron 40.0%
! GGTCTGGAGTTAACTAAAGA+TGG - chr3_2:84046576-84046595 None:intergenic 40.0%
TAGACAATGTGGACTTAGAC+TGG - chr3_2:84046597-84046616 None:intergenic 40.0%
CAGTTACAAGTTTGCATCCT+TGG + chr3_2:84046752-84046771 Msa0431920:intron 40.0%
!! AGCTGATTGTTCTGGGAAAT+TGG + chr3_2:84046792-84046811 Msa0431920:three_prime_UTR 40.0%
AACAATCTCAGAAGACCTTG+AGG - chr3_2:84046859-84046878 None:intergenic 40.0%
! TGTTTTACAGGAAGAACGAC+TGG - chr3_2:84046951-84046970 None:intergenic 40.0%
! ACCAACACTCGCTCTTTTAT+TGG + chr3_2:84047220-84047239 Msa0431920:three_prime_UTR 40.0%
TCCAATAAAAGAGCGAGTGT+TGG - chr3_2:84047224-84047243 None:intergenic 40.0%
!! TTTCGAGTTCTATTGCGTCC+AGG + chr3_2:84044058-84044077 Msa0431920:five_prime_UTR 45.0%
! TTCGAGTTCTATTGCGTCCA+GGG + chr3_2:84044059-84044078 Msa0431920:five_prime_UTR 45.0%
CTTGCGCCATTGTACAAAAC+GGG - chr3_2:84044159-84044178 None:intergenic 45.0%
! CCGATTTACCGCAGGTATTT+TGG + chr3_2:84044333-84044352 Msa0431920:intron 45.0%
GAAAGGCTCACTGAAAGAGA+AGG + chr3_2:84044396-84044415 Msa0431920:exon 45.0%
AACTACACACGATGAAGGAG+AGG - chr3_2:84044616-84044635 None:intergenic 45.0%
TGCATATTATATGCAGGGGC+CGG + chr3_2:84045221-84045240 Msa0431920:intron 45.0%
!! GGAATGCCACGATGGATTTT+TGG + chr3_2:84045450-84045469 Msa0431920:intron 45.0%
GGTTGTCCAAAAATCCATCG+TGG - chr3_2:84045459-84045478 None:intergenic 45.0%
!! GATTTTTGGACAACCACCGT+AGG + chr3_2:84045464-84045483 Msa0431920:intron 45.0%
! GTCCATTGCCCACCATATTT+TGG + chr3_2:84045922-84045941 Msa0431920:intron 45.0%
!! TCCCAAAATATGGTGGGCAA+TGG - chr3_2:84045927-84045946 None:intergenic 45.0%
GCTCGAAAAATGCACACAAC+AGG - chr3_2:84046003-84046022 None:intergenic 45.0%
! TTTCCAAGGCTGACTCAAGA+AGG - chr3_2:84046025-84046044 None:intergenic 45.0%
TCTTGCTAACTTCTGCCCTA+AGG + chr3_2:84046132-84046151 Msa0431920:intron 45.0%
CTTGCTAACTTCTGCCCTAA+GGG + chr3_2:84046133-84046152 Msa0431920:intron 45.0%
TGCCCTTAAGGCACAAGATA+AGG - chr3_2:84046278-84046297 None:intergenic 45.0%
!! TAATGAAGTGCTGCGAGTGA+TGG - chr3_2:84046436-84046455 None:intergenic 45.0%
! AATGTGGACTTAGACTGGTC+TGG - chr3_2:84046592-84046611 None:intergenic 45.0%
TCTGGGAAATTGGCAATGCT+CGG + chr3_2:84046802-84046821 Msa0431920:three_prime_UTR 45.0%
AAATTGGCAATGCTCGGAAG+TGG + chr3_2:84046808-84046827 Msa0431920:three_prime_UTR 45.0%
!!! TAAAATTGTTATTTATTTAT+GGG + chr3_2:84046109-84046128 Msa0431920:intron 5.0%
TATTGCGTCCAGGGTTCAGT+TGG + chr3_2:84044068-84044087 Msa0431920:five_prime_UTR 50.0%
ATTGCGTCCAGGGTTCAGTT+GGG + chr3_2:84044069-84044088 Msa0431920:five_prime_UTR 50.0%
TGAAAACCCCAACTGAACCC+TGG - chr3_2:84044079-84044098 None:intergenic 50.0%
GGCGCAAGATCAATACTCAC+CGG + chr3_2:84044171-84044190 Msa0431920:CDS 50.0%
GATGTAAGGGGGAATGGAAC+TGG - chr3_2:84044288-84044307 None:intergenic 50.0%
! GTGCTTTTCCGATTTACCGC+AGG + chr3_2:84044325-84044344 Msa0431920:intron 50.0%
CTCAGAAACAACCACTGCAG+AGG + chr3_2:84044372-84044391 Msa0431920:CDS 50.0%
GGCTCACTGAAAGAGAAGGT+TGG + chr3_2:84044400-84044419 Msa0431920:intron 50.0%
CTCTTACAGGGCTCATCAGT+TGG + chr3_2:84044767-84044786 Msa0431920:intron 50.0%
GGGTGCGCAATTCTCTAATC+TGG + chr3_2:84045120-84045139 Msa0431920:intron 50.0%
GCATATTATATGCAGGGGCC+GGG + chr3_2:84045222-84045241 Msa0431920:intron 50.0%
CATATTATATGCAGGGGCCG+GGG + chr3_2:84045223-84045242 Msa0431920:intron 50.0%
! TTGTGGAGAAGTGGAGTGTC+CGG - chr3_2:84045257-84045276 None:intergenic 50.0%
CCTTCACAAACTTCCTACGG+TGG - chr3_2:84045480-84045499 None:intergenic 50.0%
CCACCGTAGGAAGTTTGTGA+AGG + chr3_2:84045477-84045496 Msa0431920:intron 50.0%
TTGTGAAGGAATGGCTGCCA+TGG + chr3_2:84045491-84045510 Msa0431920:intron 50.0%
ATGTTGTCAACAGCGGATGG+CGG - chr3_2:84045566-84045585 None:intergenic 50.0%
CCAATGTTGTCAACAGCGGA+TGG - chr3_2:84045569-84045588 None:intergenic 50.0%
CCATCCGCTGTTGACAACAT+TGG + chr3_2:84045566-84045585 Msa0431920:intron 50.0%
! GAGTAGGAGTGTGGGTCTTA+TGG + chr3_2:84046469-84046488 Msa0431920:intron 50.0%
CTGATTAGTGCTGCCCTAGT+TGG - chr3_2:84046660-84046679 None:intergenic 50.0%
TTGCGTCCAGGGTTCAGTTG+GGG + chr3_2:84044070-84044089 Msa0431920:five_prime_UTR 55.0%
AGCAGCTCATGTTCTCGGTC+TGG + chr3_2:84044222-84044241 Msa0431920:CDS 55.0%
ACAACCACTGCAGAGGCGAA+AGG + chr3_2:84044379-84044398 Msa0431920:CDS 55.0%
! TGTGGAGAAGTGGAGTGTCC+GGG - chr3_2:84045256-84045275 None:intergenic 55.0%
!! TTGAAGTCATGGTGCCGCCA+TGG + chr3_2:84045426-84045445 Msa0431920:intron 55.0%
ATCGTGGCATTCCACCATGG+CGG - chr3_2:84045443-84045462 None:intergenic 55.0%
TCCATCGTGGCATTCCACCA+TGG - chr3_2:84045446-84045465 None:intergenic 55.0%
AAGGAATGGCTGCCATGGCA+GGG + chr3_2:84045496-84045515 Msa0431920:intron 55.0%
GAGCCTTCTTGAGTCAGCCT+TGG + chr3_2:84046019-84046038 Msa0431920:intron 55.0%
! ACTTCTGCCCTAAGGGCACA+AGG + chr3_2:84046140-84046159 Msa0431920:intron 55.0%
AGCACACAGGATGCACGAAC+GGG - chr3_2:84046328-84046347 None:intergenic 55.0%
CAGCACACAGGATGCACGAA+CGG - chr3_2:84046329-84046348 None:intergenic 55.0%
TGAGCCTTTCGCCTCTGCAG+TGG - chr3_2:84044386-84044405 None:intergenic 60.0%
! GTGGAGAAGTGGAGTGTCCG+GGG - chr3_2:84045255-84045274 None:intergenic 60.0%
!! AAGTCATGGTGCCGCCATGG+TGG + chr3_2:84045429-84045448 Msa0431920:intron 60.0%
! GCCATGGTGGAATGCCACGA+TGG + chr3_2:84045442-84045461 Msa0431920:intron 60.0%
GAAGGAATGGCTGCCATGGC+AGG + chr3_2:84045495-84045514 Msa0431920:intron 60.0%
!! TGTCAACAGCGGATGGCGGA+AGG - chr3_2:84045562-84045581 None:intergenic 60.0%
! TGCGGACTATCGCCCTGCCA+TGG - chr3_2:84045511-84045530 None:intergenic 65.0%
GGCAGGGCGATAGTCCGCAA+TGG + chr3_2:84045512-84045531 Msa0431920:intron 65.0%
AGTGTCCGGGGTTCGAGCCC+CGG - chr3_2:84045243-84045262 None:intergenic 70.0%
! AGGGGCCGGGGCTCGAACCC+CGG + chr3_2:84045235-84045254 Msa0431920:intron 80.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 84044054 84047300 84044054 ID=Msa0431920;Name=Msa0431920
chr3_2 mRNA 84044054 84047300 84044054 ID=Msa0431920-mRNA-1;Parent=Msa0431920;Name=Msa0431920-mRNA-1;_AED=0.14;_eAED=0.14;_QI=115|1|1|1|0.5|0.66|3|554|45
chr3_2 exon 84044054 84044243 84044054 ID=Msa0431920-mRNA-1:exon:23639;Parent=Msa0431920-mRNA-1
chr3_2 exon 84044347 84044417 84044347 ID=Msa0431920-mRNA-1:exon:23640;Parent=Msa0431920-mRNA-1
chr3_2 exon 84046755 84047300 84046755 ID=Msa0431920-mRNA-1:exon:23641;Parent=Msa0431920-mRNA-1
chr3_2 five_prime_UTR 84044054 84044168 84044054 ID=Msa0431920-mRNA-1:five_prime_utr;Parent=Msa0431920-mRNA-1
chr3_2 CDS 84044169 84044243 84044169 ID=Msa0431920-mRNA-1:cds;Parent=Msa0431920-mRNA-1
chr3_2 CDS 84044347 84044409 84044347 ID=Msa0431920-mRNA-1:cds;Parent=Msa0431920-mRNA-1
chr3_2 three_prime_UTR 84044410 84044417 84044410 ID=Msa0431920-mRNA-1:three_prime_utr;Parent=Msa0431920-mRNA-1
chr3_2 three_prime_UTR 84046755 84047300 84046755 ID=Msa0431920-mRNA-1:three_prime_utr;Parent=Msa0431920-mRNA-1
Gene Sequence

>Msa0431920

ATGGCGCAAGATCAATACTCACCGGCAGTTCCTTTATTTCTCGTTCTTCTAATAGCAGCTCATGTTCTCGGTCTGGTATTTTGGATTTATAAATTAGCTACTCAGAAACAACCACTGCAGAGGCGAAAGGCTCACTGA
Protein sequence

>Msa0431920

MAQDQYSPAVPLFLVLLIAAHVLGLVFWIYKLATQKQPLQRRKAH*