AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1393320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393320 MtrunA17_Chr2g0322371 94.730 740 38 1 1 739 1 740 0.0 1434
Msa1393320 MtrunA17_Chr2g0322361 87.600 750 82 4 1 739 1 750 0.0 1339
Msa1393320 MtrunA17_Chr4g0025201 58.658 745 294 7 1 736 1 740 0.0 875
Msa1393320 MtrunA17_Chr2g0322351 57.641 746 294 8 1 736 1 734 0.0 840
Msa1393320 MtrunA17_Chr2g0322341 65.116 344 112 4 1 336 3 346 4.39e-155 454
Msa1393320 MtrunA17_Chr4g0073711 34.658 805 447 19 1 737 228 1021 1.35e-133 421
Msa1393320 MtrunA17_Chr8g0379241 34.340 795 426 19 21 737 196 972 3.17e-132 416
Msa1393320 MtrunA17_Chr3g0088821 32.611 831 453 20 1 739 248 1063 4.84e-132 417
Msa1393320 MtrunA17_Chr2g0284211 34.430 790 418 20 21 729 181 951 4.95e-132 415
Msa1393320 MtrunA17_Chr3g0102531 33.511 752 445 14 1 727 1 722 4.58e-131 405
Msa1393320 MtrunA17_Chr3g0137951 33.831 804 430 18 21 737 281 1069 1.35e-130 414
Msa1393320 MtrunA17_Chr3g0102551 33.113 758 438 16 8 730 10 733 1.90e-130 404
Msa1393320 MtrunA17_Chr1g0183381 32.783 787 449 17 8 729 107 878 1.02e-128 404
Msa1393320 MtrunA17_Chr2g0322331 53.403 382 164 6 365 736 1 378 6.32e-128 385
Msa1393320 MtrunA17_Chr3g0102541 33.768 767 427 19 1 727 1 726 7.86e-128 397
Msa1393320 MtrunA17_Chr7g0267731 31.687 830 458 18 1 739 160 971 4.33e-127 402
Msa1393320 MtrunA17_Chr8g0364641 32.500 800 460 19 1 737 182 964 5.49e-127 402
Msa1393320 MtrunA17_Chr8g0352231 31.990 794 444 18 29 739 283 1063 6.35e-125 399
Msa1393320 MtrunA17_Chr1g0201731 32.357 819 452 19 8 737 275 1080 2.76e-123 395
Msa1393320 MtrunA17_Chr4g0046401 33.238 701 435 17 45 734 57 735 3.63e-110 351
Msa1393320 MtrunA17_Chr5g0410641 29.752 847 431 21 8 709 252 1079 3.85e-108 355
Msa1393320 MtrunA17_Chr1g0165411 29.475 838 444 19 21 729 261 1080 4.90e-107 351
Msa1393320 MtrunA17_Chr4g0039801 29.432 863 452 20 8 729 273 1119 1.65e-106 350
Msa1393320 MtrunA17_Chr2g0298581 31.329 715 443 15 16 713 8 691 2.17e-105 337
Msa1393320 MtrunA17_Chr1g0186381 30.610 771 459 20 4 738 6 736 3.20e-105 338
Msa1393320 MtrunA17_Chr1g0165421 29.433 829 425 18 26 709 276 1089 1.11e-104 345
Msa1393320 MtrunA17_Chr2g0307191 30.758 699 452 13 19 711 17 689 6.32e-104 333
Msa1393320 MtrunA17_Chr2g0297351 29.271 837 444 22 8 709 159 982 1.63e-102 337
Msa1393320 MtrunA17_Chr3g0077511 30.156 703 455 13 16 706 8 686 3.36e-102 329
Msa1393320 MtrunA17_Chr3g0095791 29.901 709 456 15 16 711 8 688 4.51e-102 328
Msa1393320 MtrunA17_Chr7g0269741 28.670 872 461 21 2 728 291 1146 1.88e-101 337
Msa1393320 MtrunA17_Chr8g0383701 41.266 395 203 9 1 368 266 658 1.01e-85 293
Msa1393320 MtrunA17_Chr8g0383701 28.982 383 238 8 372 737 714 1079 9.71e-41 161
Msa1393320 MtrunA17_Chr7g0274751 29.023 727 343 22 8 641 96 742 1.67e-84 285
Msa1393320 MtrunA17_Chr2g0296991 41.026 390 201 9 8 370 206 593 4.84e-82 282
Msa1393320 MtrunA17_Chr2g0296991 30.245 367 229 9 381 737 678 1027 2.61e-39 156
Msa1393320 MtrunA17_Chr3g0078671 40.209 383 202 9 21 378 238 618 1.02e-79 275
Msa1393320 MtrunA17_Chr3g0078671 30.028 363 239 7 381 737 679 1032 6.73e-40 158
Msa1393320 MtrunA17_Chr2g0307241 41.176 170 95 3 20 185 18 186 1.77e-33 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393320 AT2G32530.1 45.756 754 368 9 8 732 10 751 0.0 654
Msa1393320 AT2G32540.1 46.277 752 363 11 8 730 10 749 0.0 640
Msa1393320 AT4G15320.5 44.605 760 374 13 7 732 9 755 0.0 627
Msa1393320 AT4G15320.3 44.605 760 374 13 7 732 9 755 0.0 627
Msa1393320 AT4G15320.4 43.742 775 374 14 7 732 9 770 0.0 619
Msa1393320 AT2G32620.1 45.612 752 374 9 8 732 10 753 0.0 612
Msa1393320 AT2G32610.1 43.254 756 388 13 8 732 10 755 0.0 608
Msa1393320 AT4G15290.1 43.122 756 389 13 8 733 10 754 0.0 589
Msa1393320 AT2G32620.4 44.812 665 334 8 93 732 1 657 0.0 544
Msa1393320 AT4G15320.2 42.547 691 306 12 7 633 9 672 0.0 536
Msa1393320 AT2G32620.3 46.901 597 294 7 8 589 10 598 1.36e-178 524
Msa1393320 AT4G15320.1 40.191 734 305 13 7 633 9 715 8.09e-174 520
Msa1393320 AT2G32620.2 48.566 523 243 7 8 513 10 523 5.84e-160 474
Msa1393320 AT5G17420.1 34.568 810 442 20 1 737 215 1009 1.10e-135 426
Msa1393320 AT4G18780.1 34.696 807 423 17 16 737 179 966 2.37e-135 424
Msa1393320 AT4G32410.1 34.179 828 438 21 1 737 252 1063 1.27e-134 425
Msa1393320 AT5G05170.1 33.534 829 447 20 1 739 236 1050 1.39e-133 422
Msa1393320 AT5G09870.1 32.524 824 457 16 1 737 241 1052 2.72e-133 421
Msa1393320 AT2G25540.1 34.220 827 439 22 1 737 232 1043 2.79e-132 418
Msa1393320 AT1G55850.1 35.286 751 407 20 8 727 18 720 3.57e-131 406
Msa1393320 AT5G64740.1 32.169 830 455 16 1 737 253 1067 1.02e-129 412
Msa1393320 AT2G21770.1 32.807 823 456 20 1 737 259 1070 2.40e-127 405
Msa1393320 AT4G24000.1 32.168 715 438 13 27 729 34 713 4.77e-112 356
Msa1393320 AT4G23990.1 31.457 693 421 12 57 730 86 743 2.51e-111 355
Msa1393320 AT4G24010.1 33.283 658 382 14 57 696 67 685 5.05e-110 352
Msa1393320 AT1G32180.1 29.769 823 437 17 25 710 123 941 2.09e-106 347
Msa1393320 AT1G32180.2 29.769 823 437 17 25 710 123 941 2.09e-106 347
Msa1393320 AT4G38190.1 28.837 808 435 17 26 709 273 1064 7.19e-105 346
Msa1393320 AT2G33100.2 29.489 841 443 20 8 709 167 996 1.73e-104 343
Msa1393320 AT2G33100.1 29.489 841 443 20 8 709 167 996 1.73e-104 343
Msa1393320 AT1G02730.1 28.864 880 456 21 2 728 296 1158 4.24e-103 342
Msa1393320 AT3G03050.1 29.132 841 442 17 8 709 278 1103 5.23e-101 336
Msa1393320 AT5G16910.1 29.498 817 431 17 23 709 302 1103 3.06e-99 331
Msa1393320 AT4G39350.1 41.162 396 202 10 1 368 254 646 9.39e-85 290
Msa1393320 AT4G39350.1 28.451 355 241 5 386 737 722 1066 1.05e-35 145
Msa1393320 AT5G44030.1 40.256 390 204 9 8 370 199 586 4.24e-81 280
Msa1393320 AT5G44030.1 29.477 363 239 6 381 737 681 1032 1.40e-38 154
Msa1393320 AT5G44030.2 40.256 390 204 9 8 370 228 615 6.54e-81 280
Msa1393320 AT5G44030.2 29.477 363 239 6 381 737 710 1061 1.16e-38 155
Msa1393320 AT4G24000.2 31.532 555 328 13 192 729 2 521 6.03e-75 252
Msa1393320 AT5G44030.3 43.813 299 153 5 86 370 10 307 1.16e-72 252
Msa1393320 AT5G44030.3 30.056 356 232 6 388 737 409 753 5.74e-39 155
Msa1393320 AT4G23990.2 29.840 563 336 13 192 730 2 529 8.65e-72 244

Find 160 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTGGTAAAATCCAATTT+AGG 0.109508 tig0021657:+58032 Msa1393320:CDS
AGCACTTGTTATTAAGATTT+TGG 0.119897 tig0021657:+58913 Msa1393320:CDS
AACCCAAAACTTAGCAAATT+TGG 0.129090 tig0021657:-55879 None:intergenic
ATATTACTCCTGAGATTTAA+TGG 0.179687 tig0021657:+59138 Msa1393320:exon
ATGGATTTCTATGTACAAAA+TGG 0.185699 tig0021657:+59157 Msa1393320:three_prime_UTR
CTACAAGAGTATAAAGTAAA+AGG 0.191998 tig0021657:+58644 Msa1393320:CDS
CTTCCAAATTTGCTAAGTTT+TGG 0.203327 tig0021657:+55876 Msa1393320:CDS
GATTGCTTCCTCTATAAAAT+TGG 0.205458 tig0021657:-57634 None:intergenic
ATCAACAAGTGTATGGTTTA+TGG 0.206587 tig0021657:+58715 Msa1393320:CDS
TTGTAATTGTGTTCATAATT+TGG 0.215230 tig0021657:-56613 None:intergenic
CACAGAAGATATGCTATTTA+CGG 0.237161 tig0021657:+57115 Msa1393320:CDS
CCTGTTATGGGTACCCTCTT+TGG 0.238815 tig0021657:+58016 Msa1393320:CDS
CTTCAGATGGGCTGGTTATA+TGG 0.244829 tig0021657:+57812 Msa1393320:intron
AACCATTCTTGTTTGAATTC+TGG 0.245579 tig0021657:-55980 None:intergenic
AAGACAAGCCAGCCCTAAAT+TGG 0.251195 tig0021657:-58045 None:intergenic
AGTCCAACTTCTGGTGTTAT+TGG 0.253634 tig0021657:+58806 Msa1393320:CDS
TTGGAATGAAGCTGAATTTC+GGG 0.257400 tig0021657:-56199 None:intergenic
CAGGAGTAATATCAACTTAT+AGG 0.258509 tig0021657:-59127 None:intergenic
CTTATTGTTGGCTTACCAAT+TGG 0.259289 tig0021657:+58065 Msa1393320:CDS
CCCGAGCCAACCGCGTTTAC+AGG 0.262333 tig0021657:+57905 Msa1393320:CDS
TAGCAAGCATTCTCCTGTTA+TGG 0.266654 tig0021657:+58003 Msa1393320:CDS
AGTGCCATGCTTAAGCTCTT+AGG 0.270075 tig0021657:+58746 Msa1393320:CDS
CTTTGGTAAAATCCAATTTA+GGG 0.273325 tig0021657:+58033 Msa1393320:CDS
TTGCTTTCTCATGTGAATCT+TGG 0.274164 tig0021657:+55038 Msa1393320:CDS
TATCCTAATCAAATACAATA+TGG 0.281501 tig0021657:+57142 Msa1393320:CDS
AGCTTCCAAAAGACCATAAA+AGG 0.292008 tig0021657:-55855 None:intergenic
TTTCCATATTGTATTTGATT+AGG 0.299333 tig0021657:-57145 None:intergenic
CTGCAAATTCTCCCTCAAAT+TGG 0.301372 tig0021657:-56218 None:intergenic
TTGTTTGCTAGGGGTAAATA+TGG 0.302310 tig0021657:+59034 Msa1393320:CDS
TATGCGTTGACAGGTTATAT+TGG 0.311069 tig0021657:+56537 Msa1393320:intron
TTCTGGTGAATCATCACTTT+TGG 0.313648 tig0021657:-55963 None:intergenic
TGTATCTAGTAGTTTGTTAC+TGG 0.314730 tig0021657:+58996 Msa1393320:CDS
GCTTGTTATGTTTCTGATGA+TGG 0.315570 tig0021657:+55818 Msa1393320:CDS
GGAATAAAAGACGACCCTTT+TGG 0.316228 tig0021657:+56916 Msa1393320:CDS
AGCAAGCATTCTCCTGTTAT+GGG 0.321276 tig0021657:+58004 Msa1393320:CDS
ACATGCTATTTGCATATTGA+TGG 0.332778 tig0021657:+56837 Msa1393320:CDS
CAGTCTACGTTCAACATAAA+AGG 0.336254 tig0021657:-56781 None:intergenic
GAATCATCCAAGTATAATTA+AGG 0.336801 tig0021657:+56263 Msa1393320:CDS
AATAGCATGTTGCATAATCT+TGG 0.337211 tig0021657:-56824 None:intergenic
ATTGGAATGAAGCTGAATTT+CGG 0.338929 tig0021657:-56200 None:intergenic
TTATTGTTGGCTTACCAATT+GGG 0.340053 tig0021657:+58066 Msa1393320:CDS
TCAACAAGTGTATGGTTTAT+GGG 0.342595 tig0021657:+58716 Msa1393320:CDS
GGGCTGGCTTGTCTTATTGT+TGG 0.343431 tig0021657:+58053 Msa1393320:CDS
CAAATACAATATGGAAATAA+AGG 0.347147 tig0021657:+57151 Msa1393320:CDS
GCCTGTAAACGCGGTTGGCT+CGG 0.353153 tig0021657:-57906 None:intergenic
ATAATTTGGTCTCTTCTCTC+TGG 0.366584 tig0021657:-56599 None:intergenic
CATATTCACAATCAGATACT+TGG 0.373087 tig0021657:-57656 None:intergenic
TCAGCAACACAAGTTATAGA+AGG 0.377465 tig0021657:+57579 Msa1393320:CDS
ACTTGAGAGAAATATCTAAA+AGG 0.378307 tig0021657:-55938 None:intergenic
TACCAATTGGGGTCTGCGTT+CGG 0.380901 tig0021657:+58078 Msa1393320:CDS
TTCTGAAAGGGTTGTTTGCT+AGG 0.385850 tig0021657:+59023 Msa1393320:CDS
AGTGGGAAAGAGTGTGGAAT+AGG 0.391608 tig0021657:+58956 Msa1393320:CDS
TTGTTGTATTAGTACATTCT+CGG 0.396375 tig0021657:-57529 None:intergenic
ACTGTCTTATTCCAGGGAGC+TGG 0.397150 tig0021657:+58575 Msa1393320:intron
CCAAAGAGGGTACCCATAAC+AGG 0.401351 tig0021657:-58016 None:intergenic
AATGTACTAATACAACAATT+TGG 0.401655 tig0021657:+57534 Msa1393320:CDS
TTTATTACCTTAATTATACT+TGG 0.407006 tig0021657:-56270 None:intergenic
TAGCACTTGTTGGGGTAAAC+AGG 0.409962 tig0021657:+57683 Msa1393320:CDS
TCAATGGGTGGCTGCATTTG+AGG 0.415447 tig0021657:+56942 Msa1393320:CDS
TATTCCAGGGAGCTGGGCTA+TGG 0.415813 tig0021657:+58582 Msa1393320:intron
TGGATTTCTATGTACAAAAT+GGG 0.420451 tig0021657:+59158 Msa1393320:three_prime_UTR
CCTGTAAACGCGGTTGGCTC+GGG 0.421920 tig0021657:-57905 None:intergenic
ATATGAATATAGCACTTGTT+GGG 0.423823 tig0021657:+57674 Msa1393320:CDS
TGAATATGCAAAGGAAAGGT+TGG 0.428367 tig0021657:+57864 Msa1393320:CDS
GACCGAACGCAGACCCCAAT+TGG 0.428493 tig0021657:-58080 None:intergenic
CAACAAAAGAGATGGTCTTC+AGG 0.429438 tig0021657:+57962 Msa1393320:CDS
GTGCCATGCTTAAGCTCTTA+GGG 0.430274 tig0021657:+58747 Msa1393320:CDS
GCAAACAACCCTTTCAGAAA+TGG 0.433149 tig0021657:-59019 None:intergenic
TTCAGCTTCATTCCAATTTG+AGG 0.436875 tig0021657:+56206 Msa1393320:CDS
TGACTTGAAAATGGTAGATA+AGG 0.439979 tig0021657:-59062 None:intergenic
TACCGTTGAAGGAGACGGTT+TGG 0.441157 tig0021657:-55124 None:intergenic
GGACTTCAAGGACCATACTA+TGG 0.444865 tig0021657:+57076 Msa1393320:CDS
GTTACTGGCCATTTCTGAAA+GGG 0.448294 tig0021657:+59011 Msa1393320:CDS
GGGAGTGATTATTCTACTAA+TGG 0.449793 tig0021657:+57600 Msa1393320:CDS
TTTATATTACATACCGTTGA+AGG 0.450188 tig0021657:-55135 None:intergenic
CACCAGAATTCAAACAAGAA+TGG 0.451883 tig0021657:+55978 Msa1393320:CDS
GTGTTTGATCCTCCATAGTA+TGG 0.454323 tig0021657:-57088 None:intergenic
CAATAAGATCAACAAGTGTA+TGG 0.456139 tig0021657:+58708 Msa1393320:CDS
TCAGCTTCATTCCAATTTGA+GGG 0.456850 tig0021657:+56207 Msa1393320:CDS
GTTGTGCCAACCACAAAAGC+TGG 0.457175 tig0021657:-58869 None:intergenic
ATAGAAATCCATTAAATCTC+AGG 0.459476 tig0021657:-59146 None:intergenic
ATAGCATGTTGCATAATCTT+GGG 0.464154 tig0021657:-56823 None:intergenic
TTATTCTTCTCAATACCAAA+TGG 0.468145 tig0021657:+55077 Msa1393320:CDS
CATTGTAGTAAGTAATCCTC+CGG 0.471086 tig0021657:-57936 None:intergenic
GCCACCCATTGATTTCCAAA+AGG 0.473772 tig0021657:-56931 None:intergenic
ACAAGAATGGTTACAAATGA+AGG 0.473858 tig0021657:+55991 Msa1393320:CDS
TGTCAACTGCACAAGCAAAA+TGG 0.478341 tig0021657:-58891 None:intergenic
GGTAAAATCCAATTTAGGGC+TGG 0.479956 tig0021657:+58037 Msa1393320:CDS
TGTTTGCTAGGGGTAAATAT+GGG 0.481543 tig0021657:+59035 Msa1393320:CDS
GTTAACAGCAGGACTCCATT+TGG 0.481702 tig0021657:-55092 None:intergenic
ATGATTGGTGGTTCTAGAAC+AGG 0.483104 tig0021657:-55740 None:intergenic
CCACCCATTGATTTCCAAAA+GGG 0.486982 tig0021657:-56930 None:intergenic
TCTGAAAGGGTTGTTTGCTA+GGG 0.487780 tig0021657:+59024 Msa1393320:CDS
CAAAAGGAAAGTCCAACTTC+TGG 0.490074 tig0021657:+58797 Msa1393320:CDS
AATTTGCAGACAAGAGTCTC+TGG 0.491545 tig0021657:+56742 Msa1393320:intron
TAACAAGCTAGCTTATTAGC+TGG 0.493726 tig0021657:-55803 None:intergenic
TACCAACACATTCATAGCTC+CGG 0.494183 tig0021657:-56638 None:intergenic
TACAAGAGTATAAAGTAAAA+GGG 0.495125 tig0021657:+58645 Msa1393320:CDS
ATATTGGATGGTCATTCTGA+TGG 0.497574 tig0021657:+56553 Msa1393320:CDS
AATATGAATATAGCACTTGT+TGG 0.497803 tig0021657:+57673 Msa1393320:CDS
AGTCTTTGAAGTAACACAAA+AGG 0.498554 tig0021657:+58781 Msa1393320:CDS
GGTAACAAAGAAGAGAGAAT+AGG 0.498755 tig0021657:-54982 None:intergenic
ATATCAGAAGATGTACCAAC+TGG 0.500267 tig0021657:+57839 Msa1393320:CDS
GCGTTTACAGGCTGTGCTCC+CGG 0.502923 tig0021657:+57917 Msa1393320:CDS
GACTTGAAAATGGTAGATAA+GGG 0.503820 tig0021657:-59061 None:intergenic
CGTTGACAGGTTATATTGGA+TGG 0.505316 tig0021657:+56541 Msa1393320:intron
CTGTCTTATTCCAGGGAGCT+GGG 0.505938 tig0021657:+58576 Msa1393320:intron
TATTGTTGGCTTACCAATTG+GGG 0.508021 tig0021657:+58067 Msa1393320:CDS
GTTGGCCATTCTGCATATGA+TGG 0.512535 tig0021657:-54879 None:intergenic
ATATTATACTTCTTACAGAA+AGG 0.520036 tig0021657:-55902 None:intergenic
TTGTGAAGGTGTTGTTGATA+CGG 0.520835 tig0021657:-55003 None:intergenic
TGATTATGCAATATGCAACT+AGG 0.523140 tig0021657:-58123 None:intergenic
CGTAAATAGCATATCTTCTG+TGG 0.523357 tig0021657:-57114 None:intergenic
AATCTTGGTTCACATACACT+TGG 0.525534 tig0021657:+55053 Msa1393320:CDS
GCTTCCTCTATAAAATTGGA+AGG 0.528133 tig0021657:-57630 None:intergenic
ACAACAATTTGGAAGTTCAA+AGG 0.535086 tig0021657:+57545 Msa1393320:CDS
CTTTGCATATTCAATCCAGT+TGG 0.535136 tig0021657:-57854 None:intergenic
GATTATGCAATATGCAACTA+GGG 0.540386 tig0021657:-58122 None:intergenic
GTTCAACATAAAAGGAGCAT+TGG 0.542027 tig0021657:-56773 None:intergenic
ACAACACCTTCACAATTCCA+TGG 0.549695 tig0021657:+55011 Msa1393320:CDS
TGTTACTGGCCATTTCTGAA+AGG 0.550252 tig0021657:+59010 Msa1393320:CDS
TCTCCAATAACACCAGAAGT+TGG 0.551519 tig0021657:-58809 None:intergenic
TGCAATTAGAAGCTCATAGT+GGG 0.552228 tig0021657:+58939 Msa1393320:CDS
ACAGTGTTCAATGTGATGAT+TGG 0.552842 tig0021657:-55755 None:intergenic
TATGAACACAATTACAAAGC+CGG 0.562840 tig0021657:+56619 Msa1393320:CDS
AACAAAGAAGAGAGAATAGG+AGG 0.565555 tig0021657:-54979 None:intergenic
TGCTAAAGAAGACAACTGTA+AGG 0.565575 tig0021657:-57985 None:intergenic
GTGCAATTAGAAGCTCATAG+TGG 0.566989 tig0021657:+58938 Msa1393320:CDS
ATCCAAACCGTCTCCTTCAA+CGG 0.575866 tig0021657:+55122 Msa1393320:CDS
CTGAAAGGGTTGTTTGCTAG+GGG 0.575876 tig0021657:+59025 Msa1393320:CDS
AGATTAGCGATGAAATACTA+AGG 0.576860 tig0021657:-59218 None:intergenic
AATTTCCATCATATGCAGAA+TGG 0.578301 tig0021657:+54874 Msa1393320:exon
CATGAGAAAGCAAGAAACCA+TGG 0.578989 tig0021657:-55028 None:intergenic
TTGTGCCAACCACAAAAGCT+GGG 0.580530 tig0021657:-58868 None:intergenic
ATATTGATGGATTCTAAGAA+CGG 0.581409 tig0021657:+56850 Msa1393320:CDS
GTGTTCAATGTGATGATTGG+TGG 0.582750 tig0021657:-55752 None:intergenic
TTGGTGGAACAATCAGAGAA+TGG 0.583369 tig0021657:+58682 Msa1393320:CDS
CAGCAACACAAGTTATAGAA+GGG 0.583862 tig0021657:+57580 Msa1393320:CDS
GCACAGCCTGTAAACGCGGT+TGG 0.586028 tig0021657:-57911 None:intergenic
AATAGCATATCTTCTGTGGA+AGG 0.597992 tig0021657:-57110 None:intergenic
TTACATACCGTTGAAGGAGA+CGG 0.598803 tig0021657:-55129 None:intergenic
ATTGGAGATGATGCAAATGC+TGG 0.605052 tig0021657:+58824 Msa1393320:CDS
AGTACAGGAGCTTCCATGTG+TGG 0.605203 tig0021657:+55694 Msa1393320:CDS
CAATGATCCAACAAAAGAGA+TGG 0.612047 tig0021657:+57954 Msa1393320:CDS
AGCCGGAGCTATGAATGTGT+TGG 0.613715 tig0021657:+56636 Msa1393320:CDS
CAACTGGATTGAATATGCAA+AGG 0.619420 tig0021657:+57855 Msa1393320:CDS
AAGAAACCATGGAATTGTGA+AGG 0.621466 tig0021657:-55017 None:intergenic
TATGAATATAGCACTTGTTG+GGG 0.627581 tig0021657:+57675 Msa1393320:CDS
CTGGATATGTAGATTAAGTG+AGG 0.630306 tig0021657:-56580 None:intergenic
ATTGTAGTAAGTAATCCTCC+GGG 0.635850 tig0021657:-57935 None:intergenic
GGATTGAATATGCAAAGGAA+AGG 0.635952 tig0021657:+57860 Msa1393320:CDS
GCTCATAGTGGGAAAGAGTG+TGG 0.637563 tig0021657:+58950 Msa1393320:CDS
TATCCCTAAGAGCTTAAGCA+TGG 0.651527 tig0021657:-58750 None:intergenic
GGGAGCACAGCCTGTAAACG+CGG 0.667882 tig0021657:-57915 None:intergenic
CTTCAAGGACCATACTATGG+AGG 0.676748 tig0021657:+57079 Msa1393320:CDS
TTTACAGGCTGTGCTCCCGG+AGG 0.680678 tig0021657:+57920 Msa1393320:CDS
GTCACAAACAAATCCACACA+TGG 0.687294 tig0021657:-55707 None:intergenic
TGTGCCAACCACAAAAGCTG+GGG 0.694857 tig0021657:-58867 None:intergenic
GAGATGATGCAAATGCTGGT+AGG 0.699231 tig0021657:+58828 Msa1393320:CDS
GTTGAACGTAGACTGTGACA+TGG 0.762585 tig0021657:+56789 Msa1393320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTAGACAACTTTTTAA+GGG - tig0021657:55597-55616 None:intergenic 15.0%
!! TTTATTACCTTAATTATACT+TGG - tig0021657:56273-56292 None:intergenic 15.0%
!!! TTTTGGAAATAATATAACAA+CGG + tig0021657:58425-58444 Msa1393320:intron 15.0%
!! AGTTGAAAACTTGAAAATTA+TGG + tig0021657:55510-55529 Msa1393320:intron 20.0%
!!! AATTTAGACAACTTTTTAAG+GGG - tig0021657:55596-55615 None:intergenic 20.0%
!!! CAAATTTAGACAACTTTTTA+AGG - tig0021657:55598-55617 None:intergenic 20.0%
!! CTTAAAAAGTTGTCTAAATT+TGG + tig0021657:55596-55615 Msa1393320:intron 20.0%
!! AAACTAATTAGCAAGATAAA+TGG - tig0021657:55631-55650 None:intergenic 20.0%
!! ATATTATACTTCTTACAGAA+AGG - tig0021657:55905-55924 None:intergenic 20.0%
!! TATTCTCAAATACAGAAAAA+AGG + tig0021657:56241-56260 Msa1393320:CDS 20.0%
!! ATTTGTTCAATTATAGAACA+AGG - tig0021657:56340-56359 None:intergenic 20.0%
!!! ATAATTGAACAAATTTTAGC+AGG + tig0021657:56346-56365 Msa1393320:intron 20.0%
!! TTGTAATTGTGTTCATAATT+TGG - tig0021657:56616-56635 None:intergenic 20.0%
!!! TTATTCCATCGTAAAATTTT+TGG - tig0021657:56903-56922 None:intergenic 20.0%
!! TACAAACACAAATTTATAAG+TGG - tig0021657:57009-57028 None:intergenic 20.0%
!! TATCCTAATCAAATACAATA+TGG + tig0021657:57142-57161 Msa1393320:CDS 20.0%
!! TTTCCATATTGTATTTGATT+AGG - tig0021657:57148-57167 None:intergenic 20.0%
!! CAAATACAATATGGAAATAA+AGG + tig0021657:57151-57170 Msa1393320:CDS 20.0%
!!! TATTTTATCAGAATGTGATA+TGG + tig0021657:57217-57236 Msa1393320:intron 20.0%
!! AATGTACTAATACAACAATT+TGG + tig0021657:57534-57553 Msa1393320:CDS 20.0%
!!! TTGTGTAATTTTATAGTCTA+AGG - tig0021657:57751-57770 None:intergenic 20.0%
!! CATTTAATCATAAAATCAGT+TGG + tig0021657:58263-58282 Msa1393320:intron 20.0%
!! CAAAAACTGTTAAAATTAGT+CGG - tig0021657:58410-58429 None:intergenic 20.0%
!!! TACAAGAGTATAAAGTAAAA+GGG + tig0021657:58645-58664 Msa1393320:CDS 20.0%
!! TTTAATTCTATAGTTACCTA+AGG - tig0021657:59189-59208 None:intergenic 20.0%
!!! TCCATATTTTTTCATACAGA+GGG - tig0021657:54916-54935 None:intergenic 25.0%
!!! ATCCATATTTTTTCATACAG+AGG - tig0021657:54917-54936 None:intergenic 25.0%
! TTATTCTTCTCAATACCAAA+TGG + tig0021657:55077-55096 Msa1393320:CDS 25.0%
! TTTATATTACATACCGTTGA+AGG - tig0021657:55138-55157 None:intergenic 25.0%
!!! GACAACGTTTTAATAAAGTT+CGG + tig0021657:55388-55407 Msa1393320:intron 25.0%
! GTTAATGCAGATTAAAGATA+AGG - tig0021657:55574-55593 None:intergenic 25.0%
!!! TGTTCTGTTTTTACCTTTTA+TGG + tig0021657:55842-55861 Msa1393320:CDS 25.0%
!! TTTTACCTTTTATGGTCTTT+TGG + tig0021657:55850-55869 Msa1393320:CDS 25.0%
!!! TTCCAAATTTGCTAAGTTTT+GGG + tig0021657:55877-55896 Msa1393320:CDS 25.0%
! ACTTGAGAGAAATATCTAAA+AGG - tig0021657:55941-55960 None:intergenic 25.0%
! GAGATAAGTATCTAAATCTA+TGG + tig0021657:56056-56075 Msa1393320:intron 25.0%
! ATAAGTATCTAAATCTATGG+TGG + tig0021657:56059-56078 Msa1393320:intron 25.0%
! GAATCATCCAAGTATAATTA+AGG + tig0021657:56263-56282 Msa1393320:CDS 25.0%
! AGAAATACTTGGAAGAATTT+AGG - tig0021657:56400-56419 None:intergenic 25.0%
! TAAATTCTTCCAAGTATTTC+TGG + tig0021657:56399-56418 Msa1393320:intron 25.0%
! CTCATTCTTATCTTTCTTAA+CGG + tig0021657:56718-56737 Msa1393320:intron 25.0%
! TCATTCTTATCTTTCTTAAC+GGG + tig0021657:56719-56738 Msa1393320:intron 25.0%
!!! ATATTGATGGATTCTAAGAA+CGG + tig0021657:56850-56869 Msa1393320:CDS 25.0%
!! TGTTTCCAAAAATTTTACGA+TGG + tig0021657:56895-56914 Msa1393320:CDS 25.0%
! TATGCTTGAGTAATTATATG+AGG + tig0021657:57417-57436 Msa1393320:intron 25.0%
! TTGTTGTATTAGTACATTCT+CGG - tig0021657:57532-57551 None:intergenic 25.0%
! AATATGAATATAGCACTTGT+TGG + tig0021657:57673-57692 Msa1393320:CDS 25.0%
! ATATGAATATAGCACTTGTT+GGG + tig0021657:57674-57693 Msa1393320:CDS 25.0%
!!! TTATCTTTTGAAACTTCAGA+TGG + tig0021657:57799-57818 Msa1393320:intron 25.0%
!!! TATCTTTTGAAACTTCAGAT+GGG + tig0021657:57800-57819 Msa1393320:intron 25.0%
!! AAATTGGATTTTACCAAAGA+GGG - tig0021657:58032-58051 None:intergenic 25.0%
!! TAAATTGGATTTTACCAAAG+AGG - tig0021657:58033-58052 None:intergenic 25.0%
! TCTTTGGTAAAATCCAATTT+AGG + tig0021657:58032-58051 Msa1393320:CDS 25.0%
! CTTTGGTAAAATCCAATTTA+GGG + tig0021657:58033-58052 Msa1393320:CDS 25.0%
!! CTCAATGTTTAATTTAACCT+TGG - tig0021657:58200-58219 None:intergenic 25.0%
!!! CGACTAATTTTAACAGTTTT+TGG + tig0021657:58408-58427 Msa1393320:intron 25.0%
! GAAATAATATAACAACGGTT+AGG + tig0021657:58430-58449 Msa1393320:intron 25.0%
!! CTACAAGAGTATAAAGTAAA+AGG + tig0021657:58644-58663 Msa1393320:CDS 25.0%
!! AAGGGTTTTCATTAAGATAT+TGG + tig0021657:58663-58682 Msa1393320:CDS 25.0%
!!! AGCACTTGTTATTAAGATTT+TGG + tig0021657:58913-58932 Msa1393320:CDS 25.0%
!!! GCACTTGTTATTAAGATTTT+GGG + tig0021657:58914-58933 Msa1393320:CDS 25.0%
!!! CACTTGTTATTAAGATTTTG+GGG + tig0021657:58915-58934 Msa1393320:CDS 25.0%
!!! ACTTGTTATTAAGATTTTGG+GGG + tig0021657:58916-58935 Msa1393320:CDS 25.0%
! ATATTACTCCTGAGATTTAA+TGG + tig0021657:59138-59157 Msa1393320:exon 25.0%
! ATAGAAATCCATTAAATCTC+AGG - tig0021657:59149-59168 None:intergenic 25.0%
! ATGGATTTCTATGTACAAAA+TGG + tig0021657:59157-59176 Msa1393320:three_prime_UTR 25.0%
! TGGATTTCTATGTACAAAAT+GGG + tig0021657:59158-59177 Msa1393320:three_prime_UTR 25.0%
AATTTCCATCATATGCAGAA+TGG + tig0021657:54874-54893 Msa1393320:exon 30.0%
TCCCTCTGTATGAAAAAATA+TGG + tig0021657:54912-54931 Msa1393320:CDS 30.0%
TGTGACTCAATCAATAGTTA+TGG + tig0021657:55219-55238 Msa1393320:intron 30.0%
AAACTCATTAACCACCAATT+TGG - tig0021657:55546-55565 None:intergenic 30.0%
! TTTTTATCCTCATAGAGTAC+AGG + tig0021657:55679-55698 Msa1393320:intron 30.0%
!!! CTTCCAAATTTGCTAAGTTT+TGG + tig0021657:55876-55895 Msa1393320:CDS 30.0%
AACCCAAAACTTAGCAAATT+TGG - tig0021657:55882-55901 None:intergenic 30.0%
! AACCATTCTTGTTTGAATTC+TGG - tig0021657:55983-56002 None:intergenic 30.0%
ACAAGAATGGTTACAAATGA+AGG + tig0021657:55991-56010 Msa1393320:CDS 30.0%
TGCACAAGTAACAAATTTGT+AGG + tig0021657:56091-56110 Msa1393320:intron 30.0%
CAAGTAACAAATTTGTAGGA+TGG + tig0021657:56095-56114 Msa1393320:intron 30.0%
TGACAATCTTAGCCAAAAAA+TGG + tig0021657:56167-56186 Msa1393320:CDS 30.0%
ATTGGAATGAAGCTGAATTT+CGG - tig0021657:56203-56222 None:intergenic 30.0%
AACTCATATGTGAGTCAATT+AGG - tig0021657:56492-56511 None:intergenic 30.0%
TATGAACACAATTACAAAGC+CGG + tig0021657:56619-56638 Msa1393320:CDS 30.0%
TTCGCAGAGCTAAATAATAT+TGG - tig0021657:56674-56693 None:intergenic 30.0%
AGAAAGATAAGAATGAGTCT+CGG - tig0021657:56715-56734 None:intergenic 30.0%
ATAGCATGTTGCATAATCTT+GGG - tig0021657:56826-56845 None:intergenic 30.0%
AATAGCATGTTGCATAATCT+TGG - tig0021657:56827-56846 None:intergenic 30.0%
! ACATGCTATTTGCATATTGA+TGG + tig0021657:56837-56856 Msa1393320:CDS 30.0%
!!! TGTTTTTTGCAGTACATGAT+AGG + tig0021657:57043-57062 Msa1393320:intron 30.0%
!!! GTTTTTTGCAGTACATGATA+GGG + tig0021657:57044-57063 Msa1393320:intron 30.0%
!!! TTTTTTGCAGTACATGATAG+GGG + tig0021657:57045-57064 Msa1393320:intron 30.0%
! CACAGAAGATATGCTATTTA+CGG + tig0021657:57115-57134 Msa1393320:CDS 30.0%
!! TTGTTAACTGGTTCTAGTTA+AGG - tig0021657:57367-57386 None:intergenic 30.0%
! ATTGAGCTTAAGTTGTTAAC+TGG - tig0021657:57379-57398 None:intergenic 30.0%
!! CGCTATTTTGTATCTTTACT+AGG + tig0021657:57498-57517 Msa1393320:intron 30.0%
ACAACAATTTGGAAGTTCAA+AGG + tig0021657:57545-57564 Msa1393320:CDS 30.0%
! TAGTCCTTCCAATTTTATAG+AGG + tig0021657:57626-57645 Msa1393320:CDS 30.0%
GATTGCTTCCTCTATAAAAT+TGG - tig0021657:57637-57656 None:intergenic 30.0%
CATATTCACAATCAGATACT+TGG - tig0021657:57659-57678 None:intergenic 30.0%
! TATGAATATAGCACTTGTTG+GGG + tig0021657:57675-57694 Msa1393320:CDS 30.0%
!! TTATTGTTGGCTTACCAATT+GGG + tig0021657:58066-58085 Msa1393320:CDS 30.0%
GATTATGCAATATGCAACTA+GGG - tig0021657:58125-58144 None:intergenic 30.0%
TGATTATGCAATATGCAACT+AGG - tig0021657:58126-58145 None:intergenic 30.0%
TGTTAAAATTAGTCGGAGAT+TGG - tig0021657:58403-58422 None:intergenic 30.0%
! GGTTTTCATTAAGATATTGG+TGG + tig0021657:58666-58685 Msa1393320:CDS 30.0%
CAATAAGATCAACAAGTGTA+TGG + tig0021657:58708-58727 Msa1393320:CDS 30.0%
ATCAACAAGTGTATGGTTTA+TGG + tig0021657:58715-58734 Msa1393320:CDS 30.0%
TCAACAAGTGTATGGTTTAT+GGG + tig0021657:58716-58735 Msa1393320:CDS 30.0%
!! AGTCTTTGAAGTAACACAAA+AGG + tig0021657:58781-58800 Msa1393320:CDS 30.0%
!! TGTATCTAGTAGTTTGTTAC+TGG + tig0021657:58996-59015 Msa1393320:CDS 30.0%
GACTTGAAAATGGTAGATAA+GGG - tig0021657:59064-59083 None:intergenic 30.0%
TGACTTGAAAATGGTAGATA+AGG - tig0021657:59065-59084 None:intergenic 30.0%
!! AAAAAGTGCTGACTTGAAAA+TGG - tig0021657:59074-59093 None:intergenic 30.0%
CAGGAGTAATATCAACTTAT+AGG - tig0021657:59130-59149 None:intergenic 30.0%
AGATTAGCGATGAAATACTA+AGG - tig0021657:59221-59240 None:intergenic 30.0%
AACAAAGAAGAGAGAATAGG+AGG - tig0021657:54982-55001 None:intergenic 35.0%
GGTAACAAAGAAGAGAGAAT+AGG - tig0021657:54985-55004 None:intergenic 35.0%
!! TTGTGAAGGTGTTGTTGATA+CGG - tig0021657:55006-55025 None:intergenic 35.0%
AAGAAACCATGGAATTGTGA+AGG - tig0021657:55020-55039 None:intergenic 35.0%
TTGCTTTCTCATGTGAATCT+TGG + tig0021657:55038-55057 Msa1393320:CDS 35.0%
AATCTTGGTTCACATACACT+TGG + tig0021657:55053-55072 Msa1393320:CDS 35.0%
!! GGATATGTTTTGTTAACAGC+AGG - tig0021657:55106-55125 None:intergenic 35.0%
ATGTCTCAGTTTATATGTCG+CGG + tig0021657:55271-55290 Msa1393320:intron 35.0%
ATGGTTAACCATGTCCAAAT+TGG + tig0021657:55529-55548 Msa1393320:intron 35.0%
ATTAACCACCAATTTGGACA+TGG - tig0021657:55540-55559 None:intergenic 35.0%
ACAGTGTTCAATGTGATGAT+TGG - tig0021657:55758-55777 None:intergenic 35.0%
! TAACAAGCTAGCTTATTAGC+TGG - tig0021657:55806-55825 None:intergenic 35.0%
!! GCTTGTTATGTTTCTGATGA+TGG + tig0021657:55818-55837 Msa1393320:CDS 35.0%
AGCTTCCAAAAGACCATAAA+AGG - tig0021657:55858-55877 None:intergenic 35.0%
! TTCTGGTGAATCATCACTTT+TGG - tig0021657:55966-55985 None:intergenic 35.0%
CACCAGAATTCAAACAAGAA+TGG + tig0021657:55978-55997 Msa1393320:CDS 35.0%
ACACAAAGACGAATCATCAA+TGG - tig0021657:56129-56148 None:intergenic 35.0%
!!! TCGTCTTTGTGTTTTTTTGC+AGG + tig0021657:56137-56156 Msa1393320:intron 35.0%
TTGGAATGAAGCTGAATTTC+GGG - tig0021657:56202-56221 None:intergenic 35.0%
TTCAGCTTCATTCCAATTTG+AGG + tig0021657:56206-56225 Msa1393320:CDS 35.0%
TCAGCTTCATTCCAATTTGA+GGG + tig0021657:56207-56226 Msa1393320:CDS 35.0%
ATGCAAGAACCAGAAATACT+TGG - tig0021657:56411-56430 None:intergenic 35.0%
GACTAGGAATGAACTAATTG+AGG - tig0021657:56461-56480 None:intergenic 35.0%
! CAATTAGGTTCATATCGACT+AGG - tig0021657:56477-56496 None:intergenic 35.0%
! TATGCGTTGACAGGTTATAT+TGG + tig0021657:56537-56556 Msa1393320:intron 35.0%
ATATTGGATGGTCATTCTGA+TGG + tig0021657:56553-56572 Msa1393320:CDS 35.0%
CTGGATATGTAGATTAAGTG+AGG - tig0021657:56583-56602 None:intergenic 35.0%
ATAATTTGGTCTCTTCTCTC+TGG - tig0021657:56602-56621 None:intergenic 35.0%
! GTTCAACATAAAAGGAGCAT+TGG - tig0021657:56776-56795 None:intergenic 35.0%
CAGTCTACGTTCAACATAAA+AGG - tig0021657:56784-56803 None:intergenic 35.0%
! TTTTTGCAGTACATGATAGG+GGG + tig0021657:57046-57065 Msa1393320:intron 35.0%
AATAGCATATCTTCTGTGGA+AGG - tig0021657:57113-57132 None:intergenic 35.0%
CGTAAATAGCATATCTTCTG+TGG - tig0021657:57117-57136 None:intergenic 35.0%
GGACACGATTGATAAAAATG+TGG + tig0021657:57238-57257 Msa1393320:intron 35.0%
!!! CGAGTTTTTTAGTATCACAC+AGG - tig0021657:57480-57499 None:intergenic 35.0%
TCAGCAACACAAGTTATAGA+AGG + tig0021657:57579-57598 Msa1393320:CDS 35.0%
CAGCAACACAAGTTATAGAA+GGG + tig0021657:57580-57599 Msa1393320:CDS 35.0%
GGGAGTGATTATTCTACTAA+TGG + tig0021657:57600-57619 Msa1393320:CDS 35.0%
GCTTCCTCTATAAAATTGGA+AGG - tig0021657:57633-57652 None:intergenic 35.0%
ATATCAGAAGATGTACCAAC+TGG + tig0021657:57839-57858 Msa1393320:CDS 35.0%
CTTTGCATATTCAATCCAGT+TGG - tig0021657:57857-57876 None:intergenic 35.0%
CAACTGGATTGAATATGCAA+AGG + tig0021657:57855-57874 Msa1393320:CDS 35.0%
GGATTGAATATGCAAAGGAA+AGG + tig0021657:57860-57879 Msa1393320:CDS 35.0%
TGAATATGCAAAGGAAAGGT+TGG + tig0021657:57864-57883 Msa1393320:CDS 35.0%
ATTGTAGTAAGTAATCCTCC+GGG - tig0021657:57938-57957 None:intergenic 35.0%
CATTGTAGTAAGTAATCCTC+CGG - tig0021657:57939-57958 None:intergenic 35.0%
CAATGATCCAACAAAAGAGA+TGG + tig0021657:57954-57973 Msa1393320:CDS 35.0%
TGCTAAAGAAGACAACTGTA+AGG - tig0021657:57988-58007 None:intergenic 35.0%
!! CTTATTGTTGGCTTACCAAT+TGG + tig0021657:58065-58084 Msa1393320:CDS 35.0%
!! TATTGTTGGCTTACCAATTG+GGG + tig0021657:58067-58086 Msa1393320:CDS 35.0%
! TAACACCAGTATTTTTCCTG+AGG + tig0021657:58147-58166 Msa1393320:CDS 35.0%
TCTAACCTCAGGAAAAATAC+TGG - tig0021657:58155-58174 None:intergenic 35.0%
GAATGTCAATATCTAACCTC+AGG - tig0021657:58166-58185 None:intergenic 35.0%
!! ACATTCAATTTCTAGTGCCA+AGG + tig0021657:58180-58199 Msa1393320:intron 35.0%
TAACCAGCTTGCAATTAACT+AGG - tig0021657:58304-58323 None:intergenic 35.0%
!! CAAGTTGGTTAAGCTTAGTT+AGG - tig0021657:58360-58379 None:intergenic 35.0%
ATTAGTTGCAACTTGCAAGT+TGG - tig0021657:58375-58394 None:intergenic 35.0%
TGGAATAAGACAGTGTGAAA+AGG - tig0021657:58569-58588 None:intergenic 35.0%
!! TTTTCACACTGTCTTATTCC+AGG + tig0021657:58568-58587 Msa1393320:intron 35.0%
! TTTCACACTGTCTTATTCCA+GGG + tig0021657:58569-58588 Msa1393320:intron 35.0%
TGCAATTAGAAGCTCATAGT+GGG + tig0021657:58939-58958 Msa1393320:CDS 35.0%
! TTGTTTGCTAGGGGTAAATA+TGG + tig0021657:59034-59053 Msa1393320:CDS 35.0%
! TGTTTGCTAGGGGTAAATAT+GGG + tig0021657:59035-59054 Msa1393320:CDS 35.0%
TACAAAATGGGTAACACCTT+AGG + tig0021657:59170-59189 Msa1393320:intron 35.0%
! CATACAGAGGGAGATTTTCT+TGG - tig0021657:54904-54923 None:intergenic 40.0%
ACAACACCTTCACAATTCCA+TGG + tig0021657:55011-55030 Msa1393320:CDS 40.0%
CATGAGAAAGCAAGAAACCA+TGG - tig0021657:55031-55050 None:intergenic 40.0%
TTACATACCGTTGAAGGAGA+CGG - tig0021657:55132-55151 None:intergenic 40.0%
AATGATCGACGAAGTCACTT+TGG - tig0021657:55461-55480 None:intergenic 40.0%
! GACTTCGTCGATCATTGATT+TGG + tig0021657:55465-55484 Msa1393320:intron 40.0%
GTTAACCATGTCCAAATTGG+TGG + tig0021657:55532-55551 Msa1393320:intron 40.0%
GTCACAAACAAATCCACACA+TGG - tig0021657:55710-55729 None:intergenic 40.0%
!! ATGATTGGTGGTTCTAGAAC+AGG - tig0021657:55743-55762 None:intergenic 40.0%
GTGTTCAATGTGATGATTGG+TGG - tig0021657:55755-55774 None:intergenic 40.0%
! GAACACTGTTTTGTCTCTGT+TGG + tig0021657:55769-55788 Msa1393320:CDS 40.0%
!!! GGGTCACATCTTCCATTTTT+TGG - tig0021657:56182-56201 None:intergenic 40.0%
CTGCAAATTCTCCCTCAAAT+TGG - tig0021657:56221-56240 None:intergenic 40.0%
! CACTTACATTATGCGTTGAC+AGG + tig0021657:56528-56547 Msa1393320:intron 40.0%
CGTTGACAGGTTATATTGGA+TGG + tig0021657:56541-56560 Msa1393320:intron 40.0%
TACCAACACATTCATAGCTC+CGG - tig0021657:56641-56660 None:intergenic 40.0%
GAATGAGTCTCGGAAACATA+AGG - tig0021657:56705-56724 None:intergenic 40.0%
AATTTGCAGACAAGAGTCTC+TGG + tig0021657:56742-56761 Msa1393320:intron 40.0%
! GGAATAAAAGACGACCCTTT+TGG + tig0021657:56916-56935 Msa1393320:CDS 40.0%
!! ACGACCCTTTTGGAAATCAA+TGG + tig0021657:56926-56945 Msa1393320:CDS 40.0%
!! CGACCCTTTTGGAAATCAAT+GGG + tig0021657:56927-56946 Msa1393320:CDS 40.0%
!! CCACCCATTGATTTCCAAAA+GGG - tig0021657:56933-56952 None:intergenic 40.0%
GTGTTTGATCCTCCATAGTA+TGG - tig0021657:57091-57110 None:intergenic 40.0%
AGTGACAGATAGAGATAGAG+CGG + tig0021657:57298-57317 Msa1393320:intron 40.0%
!!! TTTTGAAACTTCAGATGGGC+TGG + tig0021657:57804-57823 Msa1393320:intron 40.0%
! CTGAAGACCATCTCTTTTGT+TGG - tig0021657:57964-57983 None:intergenic 40.0%
CAACAAAAGAGATGGTCTTC+AGG + tig0021657:57962-57981 Msa1393320:CDS 40.0%
TAGCAAGCATTCTCCTGTTA+TGG + tig0021657:58003-58022 Msa1393320:CDS 40.0%
AGCAAGCATTCTCCTGTTAT+GGG + tig0021657:58004-58023 Msa1393320:CDS 40.0%
! GGTAAAATCCAATTTAGGGC+TGG + tig0021657:58037-58056 Msa1393320:CDS 40.0%
GTACCTAGTTAATTGCAAGC+TGG + tig0021657:58298-58317 Msa1393320:intron 40.0%
TTGGTGGAACAATCAGAGAA+TGG + tig0021657:58682-58701 Msa1393320:CDS 40.0%
TATCCCTAAGAGCTTAAGCA+TGG - tig0021657:58753-58772 None:intergenic 40.0%
CAAAAGGAAAGTCCAACTTC+TGG + tig0021657:58797-58816 Msa1393320:CDS 40.0%
!! AGTCCAACTTCTGGTGTTAT+TGG + tig0021657:58806-58825 Msa1393320:CDS 40.0%
TCTCCAATAACACCAGAAGT+TGG - tig0021657:58812-58831 None:intergenic 40.0%
ATTGGAGATGATGCAAATGC+TGG + tig0021657:58824-58843 Msa1393320:CDS 40.0%
TGTCAACTGCACAAGCAAAA+TGG - tig0021657:58894-58913 None:intergenic 40.0%
GTGCAATTAGAAGCTCATAG+TGG + tig0021657:58938-58957 Msa1393320:CDS 40.0%
TGTTACTGGCCATTTCTGAA+AGG + tig0021657:59010-59029 Msa1393320:CDS 40.0%
! GTTACTGGCCATTTCTGAAA+GGG + tig0021657:59011-59030 Msa1393320:CDS 40.0%
GCAAACAACCCTTTCAGAAA+TGG - tig0021657:59022-59041 None:intergenic 40.0%
! TTCTGAAAGGGTTGTTTGCT+AGG + tig0021657:59023-59042 Msa1393320:CDS 40.0%
! TCTGAAAGGGTTGTTTGCTA+GGG + tig0021657:59024-59043 Msa1393320:CDS 40.0%
GTTGGCCATTCTGCATATGA+TGG - tig0021657:54882-54901 None:intergenic 45.0%
! CAGAGGGAGATTTTCTTGGT+TGG - tig0021657:54900-54919 None:intergenic 45.0%
GTTAACAGCAGGACTCCATT+TGG - tig0021657:55095-55114 None:intergenic 45.0%
ATCCAAACCGTCTCCTTCAA+CGG + tig0021657:55122-55141 Msa1393320:CDS 45.0%
ATACGCTTCGATCGTGATCA+TGG - tig0021657:55432-55451 None:intergenic 45.0%
GTTGAACGTAGACTGTGACA+TGG + tig0021657:56789-56808 Msa1393320:CDS 45.0%
!! CCCTTTTGGAAATCAATGGG+TGG + tig0021657:56930-56949 Msa1393320:CDS 45.0%
!! GCCACCCATTGATTTCCAAA+AGG - tig0021657:56934-56953 None:intergenic 45.0%
GGACTTCAAGGACCATACTA+TGG + tig0021657:57076-57095 Msa1393320:CDS 45.0%
CTTCAAGGACCATACTATGG+AGG + tig0021657:57079-57098 Msa1393320:CDS 45.0%
!! AGATAGAGCGGTGTCACATA+TGG + tig0021657:57310-57329 Msa1393320:intron 45.0%
! TAGCACTTGTTGGGGTAAAC+AGG + tig0021657:57683-57702 Msa1393320:CDS 45.0%
!! CTTCAGATGGGCTGGTTATA+TGG + tig0021657:57812-57831 Msa1393320:intron 45.0%
AAGACAAGCCAGCCCTAAAT+TGG - tig0021657:58048-58067 None:intergenic 45.0%
AGTGCCATGCTTAAGCTCTT+AGG + tig0021657:58746-58765 Msa1393320:CDS 45.0%
GTGCCATGCTTAAGCTCTTA+GGG + tig0021657:58747-58766 Msa1393320:CDS 45.0%
GAGATGATGCAAATGCTGGT+AGG + tig0021657:58828-58847 Msa1393320:CDS 45.0%
TTGTGCCAACCACAAAAGCT+GGG - tig0021657:58871-58890 None:intergenic 45.0%
! AGTGGGAAAGAGTGTGGAAT+AGG + tig0021657:58956-58975 Msa1393320:CDS 45.0%
! CTGAAAGGGTTGTTTGCTAG+GGG + tig0021657:59025-59044 Msa1393320:CDS 45.0%
TACCGTTGAAGGAGACGGTT+TGG - tig0021657:55127-55146 None:intergenic 50.0%
GGAAGCTCCTGTACTCTATG+AGG - tig0021657:55689-55708 None:intergenic 50.0%
AGTACAGGAGCTTCCATGTG+TGG + tig0021657:55694-55713 Msa1393320:CDS 50.0%
AGCCGGAGCTATGAATGTGT+TGG + tig0021657:56636-56655 Msa1393320:CDS 50.0%
TCAATGGGTGGCTGCATTTG+AGG + tig0021657:56942-56961 Msa1393320:CDS 50.0%
GCAGTACATGATAGGGGGTA+TGG + tig0021657:57051-57070 Msa1393320:intron 50.0%
! TACATGATAGGGGGTATGGC+AGG + tig0021657:57055-57074 Msa1393320:CDS 50.0%
!! CCAAAGAGGGTACCCATAAC+AGG - tig0021657:58019-58038 None:intergenic 50.0%
CCTGTTATGGGTACCCTCTT+TGG + tig0021657:58016-58035 Msa1393320:CDS 50.0%
!! GGGCTGGCTTGTCTTATTGT+TGG + tig0021657:58053-58072 Msa1393320:CDS 50.0%
TACCAATTGGGGTCTGCGTT+CGG + tig0021657:58078-58097 Msa1393320:CDS 50.0%
! ACTGTCTTATTCCAGGGAGC+TGG + tig0021657:58575-58594 Msa1393320:intron 50.0%
! CTGTCTTATTCCAGGGAGCT+GGG + tig0021657:58576-58595 Msa1393320:intron 50.0%
! TGATGAGTCCCCAGCTTTTG+TGG + tig0021657:58859-58878 Msa1393320:CDS 50.0%
TGTGCCAACCACAAAAGCTG+GGG - tig0021657:58870-58889 None:intergenic 50.0%
GTTGTGCCAACCACAAAAGC+TGG - tig0021657:58872-58891 None:intergenic 50.0%
! GCTCATAGTGGGAAAGAGTG+TGG + tig0021657:58950-58969 Msa1393320:CDS 50.0%
TATTCCAGGGAGCTGGGCTA+TGG + tig0021657:58582-58601 Msa1393320:intron 55.0%
TAATCCATAGCCCAGCTCCC+TGG - tig0021657:58589-58608 None:intergenic 55.0%
! GAGTCCCCAGCTTTTGTGGT+TGG + tig0021657:58863-58882 Msa1393320:CDS 55.0%
! GGGGGTATGGCAGGACTTCA+AGG + tig0021657:57064-57083 Msa1393320:CDS 60.0%
! CCTGTAAACGCGGTTGGCTC+GGG - tig0021657:57908-57927 None:intergenic 60.0%
! GCCTGTAAACGCGGTTGGCT+CGG - tig0021657:57909-57928 None:intergenic 60.0%
GCACAGCCTGTAAACGCGGT+TGG - tig0021657:57914-57933 None:intergenic 60.0%
! GGGAGCACAGCCTGTAAACG+CGG - tig0021657:57918-57937 None:intergenic 60.0%
GCGTTTACAGGCTGTGCTCC+CGG + tig0021657:57917-57936 Msa1393320:CDS 60.0%
TTTACAGGCTGTGCTCCCGG+AGG + tig0021657:57920-57939 Msa1393320:CDS 60.0%
GACCGAACGCAGACCCCAAT+TGG - tig0021657:58083-58102 None:intergenic 60.0%
CCCGAGCCAACCGCGTTTAC+AGG + tig0021657:57905-57924 Msa1393320:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0021657 gene 54862 59269 54862 ID=Msa1393320;Name=Msa1393320
tig0021657 mRNA 54862 59269 54862 ID=Msa1393320-mRNA-1;Parent=Msa1393320;Name=Msa1393320-mRNA-1;_AED=0.07;_eAED=0.07;_QI=31|1|1|1|0.88|0.8|10|107|739
tig0021657 exon 54862 55143 54862 ID=Msa1393320-mRNA-1:exon:13701;Parent=Msa1393320-mRNA-1
tig0021657 exon 55694 56012 55694 ID=Msa1393320-mRNA-1:exon:13702;Parent=Msa1393320-mRNA-1
tig0021657 exon 56159 56284 56159 ID=Msa1393320-mRNA-1:exon:13703;Parent=Msa1393320-mRNA-1
tig0021657 exon 56550 56657 56550 ID=Msa1393320-mRNA-1:exon:13704;Parent=Msa1393320-mRNA-1
tig0021657 exon 56751 56963 56751 ID=Msa1393320-mRNA-1:exon:13705;Parent=Msa1393320-mRNA-1
tig0021657 exon 57055 57172 57055 ID=Msa1393320-mRNA-1:exon:13706;Parent=Msa1393320-mRNA-1
tig0021657 exon 57520 57704 57520 ID=Msa1393320-mRNA-1:exon:13707;Parent=Msa1393320-mRNA-1
tig0021657 exon 57818 58168 57818 ID=Msa1393320-mRNA-1:exon:13708;Parent=Msa1393320-mRNA-1
tig0021657 exon 58590 59179 58590 ID=Msa1393320-mRNA-1:exon:13709;Parent=Msa1393320-mRNA-1
tig0021657 exon 59204 59269 59204 ID=Msa1393320-mRNA-1:exon:13710;Parent=Msa1393320-mRNA-1
tig0021657 five_prime_UTR 54862 54892 54862 ID=Msa1393320-mRNA-1:five_prime_utr;Parent=Msa1393320-mRNA-1
tig0021657 CDS 54893 55143 54893 ID=Msa1393320-mRNA-1:cds;Parent=Msa1393320-mRNA-1
tig0021657 CDS 55694 56012 55694 ID=Msa1393320-mRNA-1:cds;Parent=Msa1393320-mRNA-1
tig0021657 CDS 56159 56284 56159 ID=Msa1393320-mRNA-1:cds;Parent=Msa1393320-mRNA-1
tig0021657 CDS 56550 56657 56550 ID=Msa1393320-mRNA-1:cds;Parent=Msa1393320-mRNA-1
tig0021657 CDS 56751 56963 56751 ID=Msa1393320-mRNA-1:cds;Parent=Msa1393320-mRNA-1
tig0021657 CDS 57055 57172 57055 ID=Msa1393320-mRNA-1:cds;Parent=Msa1393320-mRNA-1
tig0021657 CDS 57520 57704 57520 ID=Msa1393320-mRNA-1:cds;Parent=Msa1393320-mRNA-1
tig0021657 CDS 57818 58168 57818 ID=Msa1393320-mRNA-1:cds;Parent=Msa1393320-mRNA-1
tig0021657 CDS 58590 59138 58590 ID=Msa1393320-mRNA-1:cds;Parent=Msa1393320-mRNA-1
tig0021657 three_prime_UTR 59139 59179 59139 ID=Msa1393320-mRNA-1:three_prime_utr;Parent=Msa1393320-mRNA-1
tig0021657 three_prime_UTR 59204 59269 59204 ID=Msa1393320-mRNA-1:three_prime_utr;Parent=Msa1393320-mRNA-1
Gene Sequence

>Msa1393320

ATGGCCAACCAAGAAAATCTCCCTCTGTATGAAAAAATATGGATCAAACGAACATTTCAAAGAGCAATAGATATCTTGATCTTACTCCTCCTATTCTCTCTTCTTTGTTACCGTATCAACAACACCTTCACAATTCCATGGTTTCTTGCTTTCTCATGTGAATCTTGGTTCACATACACTTGGATTATTCTTCTCAATACCAAATGGAGTCCTGCTGTTAACAAAACATATCCAAACCGTCTCCTTCAACGAGTACAGGAGCTTCCATGTGTGGATTTGTTTGTGACAACAGCAGATCCTGTTCTAGAACCACCAATCATCACATTGAACACTGTTTTGTCTCTGTTGGCTCTTGATTATCCAGCTAATAAGCTAGCTTGTTATGTTTCTGATGATGGCTGTTCTGTTTTTACCTTTTATGGTCTTTTGGAAGCTTCCAAATTTGCTAAGTTTTGGGTTCCTTTCTGTAAGAAGTATAATATACAAGTTAGAGCACCTTTTAGATATTTCTCTCAAGTTACCAAAAGTGATGATTCACCAGAATTCAAACAAGAATGGTTACAAATGAAGGATATGTATGACAATCTTAGCCAAAAAATGGAAGATGTGACCCGAAATTCAGCTTCATTCCAATTTGAGGGAGAATTTGCAGTATTCTCAAATACAGAAAAAAGGAATCATCCAAGTATAATTAAGGTTATATTGGATGGTCATTCTGATGGATTGCCTCACTTAATCTACATATCCAGAGAGAAGAGACCAAATTATGAACACAATTACAAAGCCGGAGCTATGAATGTGTTGACAAGAGTCTCTGGATTGATGACCAATGCTCCTTTTATGTTGAACGTAGACTGTGACATGGTTGTGAACAATCCCAAGATTATGCAACATGCTATTTGCATATTGATGGATTCTAAGAACGGAAAAGATGTTGCTTTTGTTCAATGTTTCCAAAAATTTTACGATGGAATAAAAGACGACCCTTTTGGAAATCAATGGGTGGCTGCATTTGAGTACATGATAGGGGGTATGGCAGGACTTCAAGGACCATACTATGGAGGATCAAACACCTTCCACAGAAGATATGCTATTTACGGTTTTTATCCTAATCAAATACAATATGGAAATAAAGGAAAATTAGCCGAGAATGTACTAATACAACAATTTGGAAGTTCAAAGGAGTTTATCAAATCAGCAACACAAGTTATAGAAGGGAGTGATTATTCTACTAATGGTATTAGTCCTTCCAATTTTATAGAGGAAGCAATCCAAGTATCTGATTGTGAATATGAATATAGCACTTGTTGGGGTAAACAGATGGGCTGGTTATATGGATCAATATCAGAAGATGTACCAACTGGATTGAATATGCAAAGGAAAGGTTGGAGATCAGAATGTTGTACACCCGAGCCAACCGCGTTTACAGGCTGTGCTCCCGGAGGATTACTTACTACAATGATCCAACAAAAGAGATGGTCTTCAGGCCTTACAGTTGTCTTCTTTAGCAAGCATTCTCCTGTTATGGGTACCCTCTTTGGTAAAATCCAATTTAGGGCTGGCTTGTCTTATTGTTGGCTTACCAATTGGGGTCTGCGTTCGGTCTTTGAAGTTTCTTATGCAGCCCTAGTTGCATATTGCATAATCACTAACACCAGTATTTTTCCTGAGGGAGCTGGGCTATGGATTACTTTGACTCTTTTTGTGATCTATACTATGCATACTCTACAAGAGTATAAAGTAAAAGGGTTTTCATTAAGATATTGGTGGAACAATCAGAGAATGGTTACAATAAGATCAACAAGTGTATGGTTTATGGGATTTTTGAGTGCCATGCTTAAGCTCTTAGGGATATCTGATACAGTCTTTGAAGTAACACAAAAGGAAAGTCCAACTTCTGGTGTTATTGGAGATGATGCAAATGCTGGTAGGTTCACATTTGATGAGTCCCCAGCTTTTGTGGTTGGCACAACCATTTTGCTTGTGCAGTTGACAGCACTTGTTATTAAGATTTTGGGGGTGCAATTAGAAGCTCATAGTGGGAAAGAGTGTGGAATAGGTGAATTGATGTGTAGTGTGTATCTAGTAGTTTGTTACTGGCCATTTCTGAAAGGGTTGTTTGCTAGGGGTAAATATGGGATTCCCTTATCTACCATTTTCAAGTCAGCACTTTTTGCTTTAATTTTTGTGCACTTTTGTAGAATTACTCCTATAAGTTGA
Protein sequence

>Msa1393320

MANQENLPLYEKIWIKRTFQRAIDILILLLLFSLLCYRINNTFTIPWFLAFSCESWFTYTWIILLNTKWSPAVNKTYPNRLLQRVQELPCVDLFVTTADPVLEPPIITLNTVLSLLALDYPANKLACYVSDDGCSVFTFYGLLEASKFAKFWVPFCKKYNIQVRAPFRYFSQVTKSDDSPEFKQEWLQMKDMYDNLSQKMEDVTRNSASFQFEGEFAVFSNTEKRNHPSIIKVILDGHSDGLPHLIYISREKRPNYEHNYKAGAMNVLTRVSGLMTNAPFMLNVDCDMVVNNPKIMQHAICILMDSKNGKDVAFVQCFQKFYDGIKDDPFGNQWVAAFEYMIGGMAGLQGPYYGGSNTFHRRYAIYGFYPNQIQYGNKGKLAENVLIQQFGSSKEFIKSATQVIEGSDYSTNGISPSNFIEEAIQVSDCEYEYSTCWGKQMGWLYGSISEDVPTGLNMQRKGWRSECCTPEPTAFTGCAPGGLLTTMIQQKRWSSGLTVVFFSKHSPVMGTLFGKIQFRAGLSYCWLTNWGLRSVFEVSYAALVAYCIITNTSIFPEGAGLWITLTLFVIYTMHTLQEYKVKGFSLRYWWNNQRMVTIRSTSVWFMGFLSAMLKLLGISDTVFEVTQKESPTSGVIGDDANAGRFTFDESPAFVVGTTILLVQLTALVIKILGVQLEAHSGKECGIGELMCSVYLVVCYWPFLKGLFARGKYGIPLSTIFKSALFALIFVHFCRITPIS*