AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1400050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1400050 MtrunA17_Chr8g0388871 95.745 188 5 2 1 187 1 186 1.69e-131 366
Msa1400050 MtrunA17_Chr6g0481891 76.966 178 39 2 10 186 23 199 4.55e-95 275
Msa1400050 MtrunA17_Chr1g0162171 53.261 184 85 1 3 186 41 223 7.39e-67 204
Msa1400050 MtrunA17_Chr1g0212091 60.000 160 63 1 24 183 60 218 1.30e-63 196
Msa1400050 MtrunA17_Chr1g0212181 52.071 169 80 1 18 186 51 218 2.92e-57 179
Msa1400050 MtrunA17_Chr1g0212121 52.071 169 80 1 18 186 51 218 2.15e-56 177
Msa1400050 MtrunA17_Chr1g0212171 46.409 181 96 1 6 186 42 221 1.41e-55 175
Msa1400050 MtrunA17_Chr7g0251801 41.975 162 93 1 23 184 115 275 3.16e-42 143
Msa1400050 MtrunA17_Chr3g0133221 36.538 156 97 1 28 183 102 255 1.38e-39 135
Msa1400050 MtrunA17_Chr8g0367701 37.888 161 98 1 23 183 109 267 5.42e-39 134
Msa1400050 MtrunA17_Chr1g0212111 60.000 110 43 1 75 184 47 155 9.57e-38 128
Msa1400050 MtrunA17_Chr2g0315771 35.802 162 102 2 23 183 65 225 1.45e-31 114
Msa1400050 MtrunA17_Chr1g0212131 53.333 60 28 0 125 184 3 62 1.16e-16 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1400050 AT5G23370.1 54.878 164 73 1 24 187 55 217 1.84e-59 185
Msa1400050 AT5G08350.1 54.878 164 73 1 24 187 54 216 1.18e-58 183
Msa1400050 AT5G23350.1 54.658 161 72 1 24 184 55 214 8.41e-57 178
Msa1400050 AT5G23360.1 52.174 161 76 1 24 184 47 206 8.29e-55 173
Msa1400050 AT5G13200.1 39.506 162 97 1 23 184 101 261 1.78e-40 138
Msa1400050 AT1G28200.1 37.267 161 99 1 23 183 96 254 1.25e-39 135
Msa1400050 AT1G28200.2 37.267 161 99 1 23 183 96 254 1.25e-39 135
Msa1400050 AT4G01600.3 37.654 162 101 0 22 183 24 185 9.90e-39 131
Msa1400050 AT2G22475.1 35.625 160 101 1 24 183 134 291 1.28e-38 134
Msa1400050 AT4G01600.1 37.888 161 100 0 23 183 64 224 2.79e-38 131
Msa1400050 AT4G01600.2 39.456 147 89 0 37 183 73 219 1.11e-35 125
Msa1400050 AT5G13200.2 41.584 101 59 0 23 123 101 201 4.74e-24 94.4
Msa1400050 AT2G22475.2 33.333 117 76 1 24 140 134 248 8.79e-21 86.7
Msa1400050 AT4G40100.1 31.111 135 63 1 49 183 112 216 1.34e-19 83.2

Find 50 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATGGAAATTTCTTATTTC+TGG 0.091487 tig0022414:-9616 None:intergenic
CTCACGATGATTCTGAATTT+TGG 0.150158 tig0022414:+10807 Msa1400050:CDS
TCTATCTATCTTCTTCTTGT+AGG 0.201857 tig0022414:+9510 Msa1400050:five_prime_UTR
TTTAGCAGTTGCAGTTTCTT+TGG 0.245252 tig0022414:-9791 None:intergenic
AGAAACTGCAACTGCTAAAA+TGG 0.264589 tig0022414:+9795 Msa1400050:CDS
CTTCTTCTTGTAGGTTTCAT+TGG 0.267365 tig0022414:+9519 Msa1400050:five_prime_UTR
CAATGTGCAGTAGGGCTAAA+TGG 0.279415 tig0022414:-10891 None:intergenic
AGTGTGAAGTTCTATGTAAA+TGG 0.297354 tig0022414:+10970 Msa1400050:three_prime_UTR
GATGCAGAAGAGAAGGTTAT+TGG 0.316549 tig0022414:-10424 None:intergenic
TCTGAAGGGTAAGTTGAGTT+TGG 0.345403 tig0022414:+10228 Msa1400050:CDS
CTTCATTCCTTCACTTTCCA+TGG 0.345985 tig0022414:+11062 Msa1400050:three_prime_UTR
AATCCTACATGCAAAACTTC+TGG 0.347012 tig0022414:-9845 None:intergenic
AGTTATATACATAAACTTAC+TGG 0.389837 tig0022414:+11184 Msa1400050:three_prime_UTR
TTCAGAGTCTCTGATAGTTT+AGG 0.396250 tig0022414:-10211 None:intergenic
CTGAAGGGTAAGTTGAGTTT+GGG 0.410973 tig0022414:+10229 Msa1400050:CDS
ACAACAGCTGGTCCTATTGC+TGG 0.416930 tig0022414:+10355 Msa1400050:CDS
TGAAGGGCAGAATGTGAATC+AGG 0.430005 tig0022414:+10757 Msa1400050:CDS
ACCTTCTCTTCTGCATCAAC+GGG 0.443671 tig0022414:+10430 Msa1400050:CDS
ATGAAAAGAACTCCAGCAAT+AGG 0.446868 tig0022414:-10367 None:intergenic
TGTTATATCTTGCAAACAGC+TGG 0.447335 tig0022414:-9546 None:intergenic
ATGAATTCTTCTACTTCTAC+AGG 0.454002 tig0022414:+9643 Msa1400050:CDS
TAATAATTACGTACCTTGTA+CGG 0.463291 tig0022414:-10469 None:intergenic
AACCTTCTCTTCTGCATCAA+CGG 0.469610 tig0022414:+10429 Msa1400050:CDS
AAAATCAAACAATGTGCAGT+AGG 0.473818 tig0022414:-10900 None:intergenic
GGGCTAAATGGACATTGAAA+TGG 0.476559 tig0022414:-10879 None:intergenic
TTTATGTATATAACTAATAG+TGG 0.492518 tig0022414:-11176 None:intergenic
GTGTGAAGTTCTATGTAAAT+GGG 0.492938 tig0022414:+10971 Msa1400050:three_prime_UTR
TTTATGTTTGAGATGTACAT+AGG 0.493478 tig0022414:+10937 Msa1400050:three_prime_UTR
GTAAGTTGAGTTTGGGTGCA+AGG 0.498584 tig0022414:+10236 Msa1400050:CDS
AAGATAAAAGAAGTCAATGA+AGG 0.502485 tig0022414:+10740 Msa1400050:CDS
TAGAAGAATTCATCAGTGAA+TGG 0.512531 tig0022414:-9633 None:intergenic
AGAAGGAGAGAAAGTGTTGA+AGG 0.521050 tig0022414:+10312 Msa1400050:CDS
ATAATACAAGAAGGTGGGAG+AGG 0.525733 tig0022414:+10259 Msa1400050:CDS
TGTTACTTATACACAACAGC+TGG 0.553623 tig0022414:+10343 Msa1400050:CDS
AAATTCATTGCAGTAAAACT+AGG 0.557356 tig0022414:+10187 Msa1400050:intron
AAATCAAACAATGTGCAGTA+GGG 0.570196 tig0022414:-10899 None:intergenic
TCCCGTTGATGCAGAAGAGA+AGG 0.582493 tig0022414:-10431 None:intergenic
AAGAAGAAGATAGATAGAGA+TGG 0.595965 tig0022414:-9505 None:intergenic
AGCACAAGAAAGCAACAACC+AGG 0.598769 tig0022414:+9818 Msa1400050:CDS
GCAAGGATAATACAAGAAGG+TGG 0.599451 tig0022414:+10253 Msa1400050:CDS
TAATACAAGAAGGTGGGAGA+GGG 0.599558 tig0022414:+10260 Msa1400050:CDS
TAAACTATCAGAGACTCTGA+AGG 0.601920 tig0022414:+10213 Msa1400050:CDS
GCATGTAGGATTCATGAACA+TGG 0.604739 tig0022414:+9856 Msa1400050:CDS
TCTTGTGTTCATAATCACCA+TGG 0.615060 tig0022414:-11079 None:intergenic
CAAGGATAATACAAGAAGGT+GGG 0.615352 tig0022414:+10254 Msa1400050:CDS
ATAATCACCATGGAAAGTGA+AGG 0.627057 tig0022414:-11069 None:intergenic
AGATAAAAGAAGTCAATGAA+GGG 0.635091 tig0022414:+10741 Msa1400050:CDS
GACGCGCAAGATACCGTACA+AGG 0.636816 tig0022414:+10456 Msa1400050:CDS
AAACTATCAGAGACTCTGAA+GGG 0.658037 tig0022414:+10214 Msa1400050:CDS
GGTGCAAGGATAATACAAGA+AGG 0.670404 tig0022414:+10250 Msa1400050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATCAATATTTTTTTTAG+GGG + tig0022414:10091-10110 Msa1400050:intron 10.0%
!!! ATAATCAATATTTTTTTTAG+GGG + tig0022414:10091-10110 Msa1400050:intron 10.0%
!!! TTTTGAATGTAATTAATTGA+AGG - tig0022414:9697-9716 None:intergenic 15.0%
!! TTAAGAGAGAAAAAAAAAAA+AGG - tig0022414:10132-10151 None:intergenic 15.0%
!!! AACATTTTCAAGAATATTTT+TGG + tig0022414:10283-10302 Msa1400050:CDS 15.0%
!!! ATAATTTGGTTTATTGATTT+AGG + tig0022414:10561-10580 Msa1400050:intron 15.0%
!!! TATAATTTTATACATGCATT+TGG - tig0022414:11140-11159 None:intergenic 15.0%
!! TTTATGTATATAACTAATAG+TGG - tig0022414:11179-11198 None:intergenic 15.0%
!!! TTTTGAATGTAATTAATTGA+AGG - tig0022414:9697-9716 None:intergenic 15.0%
!! TTAAGAGAGAAAAAAAAAAA+AGG - tig0022414:10132-10151 None:intergenic 15.0%
!!! AACATTTTCAAGAATATTTT+TGG + tig0022414:10283-10302 Msa1400050:CDS 15.0%
!!! ATAATTTGGTTTATTGATTT+AGG + tig0022414:10561-10580 Msa1400050:intron 15.0%
!!! TATAATTTTATACATGCATT+TGG - tig0022414:11140-11159 None:intergenic 15.0%
!! TTTATGTATATAACTAATAG+TGG - tig0022414:11179-11198 None:intergenic 15.0%
!!! TTGATTTGCACTTTATAATT+TGG + tig0022414:10547-10566 Msa1400050:intron 20.0%
!!! ATTTGTTGTATTAACTGTTT+CGG + tig0022414:10663-10682 Msa1400050:intron 20.0%
!! AGTTATATACATAAACTTAC+TGG + tig0022414:11184-11203 Msa1400050:three_prime_UTR 20.0%
!!! TTGATTTGCACTTTATAATT+TGG + tig0022414:10547-10566 Msa1400050:intron 20.0%
!!! ATTTGTTGTATTAACTGTTT+CGG + tig0022414:10663-10682 Msa1400050:intron 20.0%
!! AGTTATATACATAAACTTAC+TGG + tig0022414:11184-11203 Msa1400050:three_prime_UTR 20.0%
!! GAATGGAAATTTCTTATTTC+TGG - tig0022414:9619-9638 None:intergenic 25.0%
! ATGTAATTAATTGAAGGTTG+TGG - tig0022414:9691-9710 None:intergenic 25.0%
! ATACTACTAATATATTGACG+TGG + tig0022414:9753-9772 Msa1400050:intron 25.0%
! TATTTACCTCACAAATTAAC+TGG + tig0022414:9896-9915 Msa1400050:intron 25.0%
!! TAGATTAGTTATGTGTTAGT+TGG + tig0022414:10018-10037 Msa1400050:intron 25.0%
! AAATTCATTGCAGTAAAACT+AGG + tig0022414:10187-10206 Msa1400050:intron 25.0%
!!! AATATTTTTGGTATGCAAGA+AGG + tig0022414:10295-10314 Msa1400050:CDS 25.0%
!! ACTACAAAATGCAACTTTTT+GGG - tig0022414:10398-10417 None:intergenic 25.0%
! TAATAATTACGTACCTTGTA+CGG - tig0022414:10472-10491 None:intergenic 25.0%
! AGTTTGATATGTTAAAGTTG+AGG - tig0022414:10628-10647 None:intergenic 25.0%
! AATACAACAAATGTGATTAC+TGG - tig0022414:10655-10674 None:intergenic 25.0%
! AAGATAAAAGAAGTCAATGA+AGG + tig0022414:10740-10759 Msa1400050:CDS 25.0%
! AGATAAAAGAAGTCAATGAA+GGG + tig0022414:10741-10760 Msa1400050:CDS 25.0%
!!! TGATTCTGAATTTTGGTTTA+TGG + tig0022414:10814-10833 Msa1400050:CDS 25.0%
!!! GATTCTGAATTTTGGTTTAT+GGG + tig0022414:10815-10834 Msa1400050:CDS 25.0%
!! TTTATGTTTGAGATGTACAT+AGG + tig0022414:10937-10956 Msa1400050:three_prime_UTR 25.0%
!!! TTTCTTTTCTTGTTTTCACA+TGG - tig0022414:11117-11136 None:intergenic 25.0%
!! GAATGGAAATTTCTTATTTC+TGG - tig0022414:9619-9638 None:intergenic 25.0%
! ATGTAATTAATTGAAGGTTG+TGG - tig0022414:9691-9710 None:intergenic 25.0%
! ATACTACTAATATATTGACG+TGG + tig0022414:9753-9772 Msa1400050:intron 25.0%
! TATTTACCTCACAAATTAAC+TGG + tig0022414:9896-9915 Msa1400050:intron 25.0%
!! TAGATTAGTTATGTGTTAGT+TGG + tig0022414:10018-10037 Msa1400050:intron 25.0%
! AAATTCATTGCAGTAAAACT+AGG + tig0022414:10187-10206 Msa1400050:intron 25.0%
!!! AATATTTTTGGTATGCAAGA+AGG + tig0022414:10295-10314 Msa1400050:CDS 25.0%
!! ACTACAAAATGCAACTTTTT+GGG - tig0022414:10398-10417 None:intergenic 25.0%
! TAATAATTACGTACCTTGTA+CGG - tig0022414:10472-10491 None:intergenic 25.0%
! AGTTTGATATGTTAAAGTTG+AGG - tig0022414:10628-10647 None:intergenic 25.0%
! AATACAACAAATGTGATTAC+TGG - tig0022414:10655-10674 None:intergenic 25.0%
! AAGATAAAAGAAGTCAATGA+AGG + tig0022414:10740-10759 Msa1400050:CDS 25.0%
! AGATAAAAGAAGTCAATGAA+GGG + tig0022414:10741-10760 Msa1400050:CDS 25.0%
!!! TGATTCTGAATTTTGGTTTA+TGG + tig0022414:10814-10833 Msa1400050:CDS 25.0%
!!! GATTCTGAATTTTGGTTTAT+GGG + tig0022414:10815-10834 Msa1400050:CDS 25.0%
!! TTTATGTTTGAGATGTACAT+AGG + tig0022414:10937-10956 Msa1400050:three_prime_UTR 25.0%
!!! TTTCTTTTCTTGTTTTCACA+TGG - tig0022414:11117-11136 None:intergenic 25.0%
AAGAAGAAGATAGATAGAGA+TGG - tig0022414:9508-9527 None:intergenic 30.0%
TCTATCTATCTTCTTCTTGT+AGG + tig0022414:9510-9529 Msa1400050:five_prime_UTR 30.0%
TAGAAGAATTCATCAGTGAA+TGG - tig0022414:9636-9655 None:intergenic 30.0%
ATGAATTCTTCTACTTCTAC+AGG + tig0022414:9643-9662 Msa1400050:CDS 30.0%
TGTGAGGTAAATATTTAGAG+AGG - tig0022414:9889-9908 None:intergenic 30.0%
! CACTACAAAATGCAACTTTT+TGG - tig0022414:10399-10418 None:intergenic 30.0%
CTCATAAGCTCTATTACTAT+TGG - tig0022414:10522-10541 None:intergenic 30.0%
ATAGTAATAGAGCTTATGAG+TGG + tig0022414:10522-10541 Msa1400050:intron 30.0%
TGATATGTTAAAGTTGAGGT+CGG - tig0022414:10624-10643 None:intergenic 30.0%
TATTAACTGTTTCGGTATCA+AGG + tig0022414:10671-10690 Msa1400050:intron 30.0%
TCGGTATCAAGGATTTAATT+AGG + tig0022414:10682-10701 Msa1400050:intron 30.0%
! ACTTCTTTTATCTTCCCAAT+AGG - tig0022414:10734-10753 None:intergenic 30.0%
AAATCAAACAATGTGCAGTA+GGG - tig0022414:10902-10921 None:intergenic 30.0%
AAAATCAAACAATGTGCAGT+AGG - tig0022414:10903-10922 None:intergenic 30.0%
AGTGTGAAGTTCTATGTAAA+TGG + tig0022414:10970-10989 Msa1400050:three_prime_UTR 30.0%
GTGTGAAGTTCTATGTAAAT+GGG + tig0022414:10971-10990 Msa1400050:three_prime_UTR 30.0%
AAGAAGAAGATAGATAGAGA+TGG - tig0022414:9508-9527 None:intergenic 30.0%
TCTATCTATCTTCTTCTTGT+AGG + tig0022414:9510-9529 Msa1400050:five_prime_UTR 30.0%
TAGAAGAATTCATCAGTGAA+TGG - tig0022414:9636-9655 None:intergenic 30.0%
ATGAATTCTTCTACTTCTAC+AGG + tig0022414:9643-9662 Msa1400050:CDS 30.0%
TGTGAGGTAAATATTTAGAG+AGG - tig0022414:9889-9908 None:intergenic 30.0%
! CACTACAAAATGCAACTTTT+TGG - tig0022414:10399-10418 None:intergenic 30.0%
CTCATAAGCTCTATTACTAT+TGG - tig0022414:10522-10541 None:intergenic 30.0%
ATAGTAATAGAGCTTATGAG+TGG + tig0022414:10522-10541 Msa1400050:intron 30.0%
TGATATGTTAAAGTTGAGGT+CGG - tig0022414:10624-10643 None:intergenic 30.0%
TATTAACTGTTTCGGTATCA+AGG + tig0022414:10671-10690 Msa1400050:intron 30.0%
TCGGTATCAAGGATTTAATT+AGG + tig0022414:10682-10701 Msa1400050:intron 30.0%
! ACTTCTTTTATCTTCCCAAT+AGG - tig0022414:10734-10753 None:intergenic 30.0%
AAATCAAACAATGTGCAGTA+GGG - tig0022414:10902-10921 None:intergenic 30.0%
AAAATCAAACAATGTGCAGT+AGG - tig0022414:10903-10922 None:intergenic 30.0%
AGTGTGAAGTTCTATGTAAA+TGG + tig0022414:10970-10989 Msa1400050:three_prime_UTR 30.0%
GTGTGAAGTTCTATGTAAAT+GGG + tig0022414:10971-10990 Msa1400050:three_prime_UTR 30.0%
CTTCTTCTTGTAGGTTTCAT+TGG + tig0022414:9519-9538 Msa1400050:five_prime_UTR 35.0%
TGTTATATCTTGCAAACAGC+TGG - tig0022414:9549-9568 None:intergenic 35.0%
TTTAGCAGTTGCAGTTTCTT+TGG - tig0022414:9794-9813 None:intergenic 35.0%
AGAAACTGCAACTGCTAAAA+TGG + tig0022414:9795-9814 Msa1400050:CDS 35.0%
! AAACCAGAAGTTTTGCATGT+AGG + tig0022414:9842-9861 Msa1400050:CDS 35.0%
AATCCTACATGCAAAACTTC+TGG - tig0022414:9848-9867 None:intergenic 35.0%
! TATGTGTTAGTTGGTCTACT+TGG + tig0022414:10027-10046 Msa1400050:intron 35.0%
TTCAGAGTCTCTGATAGTTT+AGG - tig0022414:10214-10233 None:intergenic 35.0%
TAAACTATCAGAGACTCTGA+AGG + tig0022414:10213-10232 Msa1400050:CDS 35.0%
AAACTATCAGAGACTCTGAA+GGG + tig0022414:10214-10233 Msa1400050:CDS 35.0%
CAAGGATAATACAAGAAGGT+GGG + tig0022414:10254-10273 Msa1400050:CDS 35.0%
! TGTTACTTATACACAACAGC+TGG + tig0022414:10343-10362 Msa1400050:CDS 35.0%
ATGAAAAGAACTCCAGCAAT+AGG - tig0022414:10370-10389 None:intergenic 35.0%
!! ACAAAATGCAACTTTTTGGG+TGG - tig0022414:10395-10414 None:intergenic 35.0%
! TTAGGTGCATTTTACTATGC+AGG + tig0022414:10700-10719 Msa1400050:intron 35.0%
!! ATGCAGGTTTTGATACCTAT+TGG + tig0022414:10716-10735 Msa1400050:intron 35.0%
!! TGCAGGTTTTGATACCTATT+GGG + tig0022414:10717-10736 Msa1400050:intron 35.0%
! CTCACGATGATTCTGAATTT+TGG + tig0022414:10807-10826 Msa1400050:CDS 35.0%
! TACGCTATGAGAAAGCTTTT+AGG + tig0022414:10843-10862 Msa1400050:CDS 35.0%
ATAATCACCATGGAAAGTGA+AGG - tig0022414:11072-11091 None:intergenic 35.0%
TCTTGTGTTCATAATCACCA+TGG - tig0022414:11082-11101 None:intergenic 35.0%
CTTCTTCTTGTAGGTTTCAT+TGG + tig0022414:9519-9538 Msa1400050:five_prime_UTR 35.0%
TGTTATATCTTGCAAACAGC+TGG - tig0022414:9549-9568 None:intergenic 35.0%
TTTAGCAGTTGCAGTTTCTT+TGG - tig0022414:9794-9813 None:intergenic 35.0%
AGAAACTGCAACTGCTAAAA+TGG + tig0022414:9795-9814 Msa1400050:CDS 35.0%
! AAACCAGAAGTTTTGCATGT+AGG + tig0022414:9842-9861 Msa1400050:CDS 35.0%
AATCCTACATGCAAAACTTC+TGG - tig0022414:9848-9867 None:intergenic 35.0%
! TATGTGTTAGTTGGTCTACT+TGG + tig0022414:10027-10046 Msa1400050:intron 35.0%
TTCAGAGTCTCTGATAGTTT+AGG - tig0022414:10214-10233 None:intergenic 35.0%
TAAACTATCAGAGACTCTGA+AGG + tig0022414:10213-10232 Msa1400050:CDS 35.0%
AAACTATCAGAGACTCTGAA+GGG + tig0022414:10214-10233 Msa1400050:CDS 35.0%
CAAGGATAATACAAGAAGGT+GGG + tig0022414:10254-10273 Msa1400050:CDS 35.0%
! TGTTACTTATACACAACAGC+TGG + tig0022414:10343-10362 Msa1400050:CDS 35.0%
ATGAAAAGAACTCCAGCAAT+AGG - tig0022414:10370-10389 None:intergenic 35.0%
!! ACAAAATGCAACTTTTTGGG+TGG - tig0022414:10395-10414 None:intergenic 35.0%
! TTAGGTGCATTTTACTATGC+AGG + tig0022414:10700-10719 Msa1400050:intron 35.0%
!! ATGCAGGTTTTGATACCTAT+TGG + tig0022414:10716-10735 Msa1400050:intron 35.0%
!! TGCAGGTTTTGATACCTATT+GGG + tig0022414:10717-10736 Msa1400050:intron 35.0%
! CTCACGATGATTCTGAATTT+TGG + tig0022414:10807-10826 Msa1400050:CDS 35.0%
! TACGCTATGAGAAAGCTTTT+AGG + tig0022414:10843-10862 Msa1400050:CDS 35.0%
ATAATCACCATGGAAAGTGA+AGG - tig0022414:11072-11091 None:intergenic 35.0%
TCTTGTGTTCATAATCACCA+TGG - tig0022414:11082-11101 None:intergenic 35.0%
!!! TGCAAAACTTCTGGTTTTCC+TGG - tig0022414:9839-9858 None:intergenic 40.0%
GCATGTAGGATTCATGAACA+TGG + tig0022414:9856-9875 Msa1400050:CDS 40.0%
!! CAGTAGCCAGTTAATTTGTG+AGG - tig0022414:9905-9924 None:intergenic 40.0%
! TCTGAAGGGTAAGTTGAGTT+TGG + tig0022414:10228-10247 Msa1400050:CDS 40.0%
! CTGAAGGGTAAGTTGAGTTT+GGG + tig0022414:10229-10248 Msa1400050:CDS 40.0%
GGTGCAAGGATAATACAAGA+AGG + tig0022414:10250-10269 Msa1400050:CDS 40.0%
GCAAGGATAATACAAGAAGG+TGG + tig0022414:10253-10272 Msa1400050:CDS 40.0%
ATAATACAAGAAGGTGGGAG+AGG + tig0022414:10259-10278 Msa1400050:CDS 40.0%
TAATACAAGAAGGTGGGAGA+GGG + tig0022414:10260-10279 Msa1400050:CDS 40.0%
!! AGAAGGAGAGAAAGTGTTGA+AGG + tig0022414:10312-10331 Msa1400050:CDS 40.0%
GATGCAGAAGAGAAGGTTAT+TGG - tig0022414:10427-10446 None:intergenic 40.0%
AACCTTCTCTTCTGCATCAA+CGG + tig0022414:10429-10448 Msa1400050:CDS 40.0%
GGGCTAAATGGACATTGAAA+TGG - tig0022414:10882-10901 None:intergenic 40.0%
CTTCATTCCTTCACTTTCCA+TGG + tig0022414:11062-11081 Msa1400050:three_prime_UTR 40.0%
!!! TGCAAAACTTCTGGTTTTCC+TGG - tig0022414:9839-9858 None:intergenic 40.0%
GCATGTAGGATTCATGAACA+TGG + tig0022414:9856-9875 Msa1400050:CDS 40.0%
!! CAGTAGCCAGTTAATTTGTG+AGG - tig0022414:9905-9924 None:intergenic 40.0%
! TCTGAAGGGTAAGTTGAGTT+TGG + tig0022414:10228-10247 Msa1400050:CDS 40.0%
! CTGAAGGGTAAGTTGAGTTT+GGG + tig0022414:10229-10248 Msa1400050:CDS 40.0%
GGTGCAAGGATAATACAAGA+AGG + tig0022414:10250-10269 Msa1400050:CDS 40.0%
GCAAGGATAATACAAGAAGG+TGG + tig0022414:10253-10272 Msa1400050:CDS 40.0%
ATAATACAAGAAGGTGGGAG+AGG + tig0022414:10259-10278 Msa1400050:CDS 40.0%
TAATACAAGAAGGTGGGAGA+GGG + tig0022414:10260-10279 Msa1400050:CDS 40.0%
!! AGAAGGAGAGAAAGTGTTGA+AGG + tig0022414:10312-10331 Msa1400050:CDS 40.0%
GATGCAGAAGAGAAGGTTAT+TGG - tig0022414:10427-10446 None:intergenic 40.0%
AACCTTCTCTTCTGCATCAA+CGG + tig0022414:10429-10448 Msa1400050:CDS 40.0%
GGGCTAAATGGACATTGAAA+TGG - tig0022414:10882-10901 None:intergenic 40.0%
CTTCATTCCTTCACTTTCCA+TGG + tig0022414:11062-11081 Msa1400050:three_prime_UTR 40.0%
AGCACAAGAAAGCAACAACC+AGG + tig0022414:9818-9837 Msa1400050:CDS 45.0%
! GTAAGTTGAGTTTGGGTGCA+AGG + tig0022414:10236-10255 Msa1400050:CDS 45.0%
ACCTTCTCTTCTGCATCAAC+GGG + tig0022414:10430-10449 Msa1400050:CDS 45.0%
TGAAGGGCAGAATGTGAATC+AGG + tig0022414:10757-10776 Msa1400050:CDS 45.0%
CAATGTGCAGTAGGGCTAAA+TGG - tig0022414:10894-10913 None:intergenic 45.0%
AGCACAAGAAAGCAACAACC+AGG + tig0022414:9818-9837 Msa1400050:CDS 45.0%
! GTAAGTTGAGTTTGGGTGCA+AGG + tig0022414:10236-10255 Msa1400050:CDS 45.0%
ACCTTCTCTTCTGCATCAAC+GGG + tig0022414:10430-10449 Msa1400050:CDS 45.0%
TGAAGGGCAGAATGTGAATC+AGG + tig0022414:10757-10776 Msa1400050:CDS 45.0%
CAATGTGCAGTAGGGCTAAA+TGG - tig0022414:10894-10913 None:intergenic 45.0%
!!! TAATAATCAATATTTTTTTT+AGG + tig0022414:10089-10108 Msa1400050:intron 5.0%
!!! AATAATCAATATTTTTTTTA+GGG + tig0022414:10090-10109 Msa1400050:intron 5.0%
!!! TAATAATCAATATTTTTTTT+AGG + tig0022414:10089-10108 Msa1400050:intron 5.0%
!!! AATAATCAATATTTTTTTTA+GGG + tig0022414:10090-10109 Msa1400050:intron 5.0%
ACAACAGCTGGTCCTATTGC+TGG + tig0022414:10355-10374 Msa1400050:CDS 50.0%
TCCCGTTGATGCAGAAGAGA+AGG - tig0022414:10434-10453 None:intergenic 50.0%
ACAACAGCTGGTCCTATTGC+TGG + tig0022414:10355-10374 Msa1400050:CDS 50.0%
TCCCGTTGATGCAGAAGAGA+AGG - tig0022414:10434-10453 None:intergenic 50.0%
GACGCGCAAGATACCGTACA+AGG + tig0022414:10456-10475 Msa1400050:CDS 55.0%
GACGCGCAAGATACCGTACA+AGG + tig0022414:10456-10475 Msa1400050:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0022414 gene 9499 11211 9499 ID=Msa1400050;Name=Msa1400050
tig0022414 mRNA 9499 11211 9499 ID=Msa1400050-mRNA-1;Parent=Msa1400050;Name=Msa1400050-mRNA-1;_AED=0.07;_eAED=0.07;_QI=144|1|1|1|1|1|4|313|187
tig0022414 exon 9499 9664 9499 ID=Msa1400050-mRNA-1:exon:17540;Parent=Msa1400050-mRNA-1
tig0022414 exon 9791 9877 9791 ID=Msa1400050-mRNA-1:exon:17541;Parent=Msa1400050-mRNA-1
tig0022414 exon 10200 10477 10200 ID=Msa1400050-mRNA-1:exon:17542;Parent=Msa1400050-mRNA-1
tig0022414 exon 10722 11211 10722 ID=Msa1400050-mRNA-1:exon:17543;Parent=Msa1400050-mRNA-1
tig0022414 five_prime_UTR 9499 9642 9499 ID=Msa1400050-mRNA-1:five_prime_utr;Parent=Msa1400050-mRNA-1
tig0022414 CDS 9643 9664 9643 ID=Msa1400050-mRNA-1:cds;Parent=Msa1400050-mRNA-1
tig0022414 CDS 9791 9877 9791 ID=Msa1400050-mRNA-1:cds;Parent=Msa1400050-mRNA-1
tig0022414 CDS 10200 10477 10200 ID=Msa1400050-mRNA-1:cds;Parent=Msa1400050-mRNA-1
tig0022414 CDS 10722 10898 10722 ID=Msa1400050-mRNA-1:cds;Parent=Msa1400050-mRNA-1
tig0022414 three_prime_UTR 10899 11211 10899 ID=Msa1400050-mRNA-1:three_prime_utr;Parent=Msa1400050-mRNA-1
Gene Sequence

>Msa1400050

ATGAATTCTTCTACTTCTACAGCCAAAGAAACTGCAACTGCTAAAATGGAGCACAAGAAAGCAACAACCAGGAAAACCAGAAGTTTTGCATGTAGGATTCATGAACATGTAAAACTAGGTCCTAAACTATCAGAGACTCTGAAGGGTAAGTTGAGTTTGGGTGCAAGGATAATACAAGAAGGTGGGAGAGGGAACATTTTCAAGAATATTTTTGGTATGCAAGAAGGAGAGAAAGTGTTGAAGGCTTCACAATGTTACTTATACACAACAGCTGGTCCTATTGCTGGAGTTCTTTTCATCTCCACCCAAAAAGTTGCATTTTGTAGTGAAAGACCAATAACCTTCTCTTCTGCATCAACGGGAGAGACGCGCAAGATACCGTACAAGGTTTTGATACCTATTGGGAAGATAAAAGAAGTCAATGAAGGGCAGAATGTGAATCAGGAAGAACAAAAGTATATAGAAATTGTTACTCACGATGATTCTGAATTTTGGTTTATGGGATTTTTACGCTATGAGAAAGCTTTTAGGAATCTTCAAAAAGCCATTTCAATGTCCATTTAG
Protein sequence

>Msa1400050

MNSSTSTAKETATAKMEHKKATTRKTRSFACRIHEHVKLGPKLSETLKGKLSLGARIIQEGGRGNIFKNIFGMQEGEKVLKASQCYLYTTAGPIAGVLFISTQKVAFCSERPITFSSASTGETRKIPYKVLIPIGKIKEVNEGQNVNQEEQKYIEIVTHDDSEFWFMGFLRYEKAFRNLQKAISMSI*