AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1402410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1402410 MtrunA17_Chr1g0183851 100.000 164 0 0 1 164 1 164 1.17e-120 337
Msa1402410 MtrunA17_Chr7g0268461 99.390 164 1 0 1 164 1 164 1.81e-120 336
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1402410 AT1G09590.1 81.098 164 31 0 1 164 1 164 3.58e-101 288
Msa1402410 AT1G09690.1 81.098 164 31 0 1 164 1 164 3.58e-101 288
Msa1402410 AT1G57660.1 80.488 164 32 0 1 164 1 164 1.08e-100 286
Msa1402410 AT1G57860.1 80.488 164 32 0 1 164 1 164 1.08e-100 286

Find 71 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAACCTAGGGTTTCTTAT+TGG 0.209419 tig0022876:-24026 None:intergenic
GGCATCTTTCTTCTCCTTCT+AGG 0.223627 tig0022876:-24117 None:intergenic
TGAACCTAGGGTTTCTTATT+GGG 0.253215 tig0022876:-24025 None:intergenic
ACTCTTTCTCTCGTCCATTC+AGG 0.293911 tig0022876:+24172 Msa1402410:CDS
AAACCAGGTTTAGGTCCTTC+TGG 0.327783 tig0022876:-25080 None:intergenic
TCGGCGATTATGTCGATATC+AGG 0.353109 tig0022876:+24241 Msa1402410:CDS
AGGACCTAAACCTGGTTTCA+TGG 0.367201 tig0022876:+25085 Msa1402410:CDS
TGATGTGGTTAATGATCTTA+AGG 0.381458 tig0022876:+25148 Msa1402410:CDS
GGTTAACGGTGCCGTTCATA+AGG 0.392360 tig0022876:+24263 Msa1402410:CDS
TCAACCATGAAACCAGGTTT+AGG 0.394105 tig0022876:-25089 None:intergenic
TTAACCACATCATATGGAAT+TGG 0.408404 tig0022876:-25137 None:intergenic
GGTTTATTCGTGTTGCTTGT+GGG 0.408869 tig0022876:+25351 Msa1402410:three_prime_UTR
CTCAACACCAATAGCACGTT+TGG 0.409541 tig0022876:-24337 None:intergenic
CTCTCGTCCATTCAGGAAGA+AGG 0.411770 tig0022876:+24179 Msa1402410:CDS
AACTCCTCGGTGCACCTAGA+TGG 0.414621 tig0022876:-24978 None:intergenic
GACATAATCGCCGATGTGAT+AGG 0.419105 tig0022876:-24232 None:intergenic
AGATCATTAACCACATCATA+TGG 0.425684 tig0022876:-25143 None:intergenic
ATGGGCGTACCGGTCGTGTC+TGG 0.430669 tig0022876:+24307 Msa1402410:CDS
GCATCTTTCTTCTCCTTCTA+GGG 0.447415 tig0022876:-24116 None:intergenic
ATTGTACCCTTCTTCCTGAA+TGG 0.450869 tig0022876:-24186 None:intergenic
AAACCTGGTTTCATGGTTGA+AGG 0.456376 tig0022876:+25092 Msa1402410:CDS
CATTCTTAATCTTCCTCAAA+CGG 0.463824 tig0022876:-25001 None:intergenic
TGGTTTATTCGTGTTGCTTG+TGG 0.469806 tig0022876:+25350 Msa1402410:three_prime_UTR
AACGTTACCAAACGTGCTAT+TGG 0.472288 tig0022876:+24330 Msa1402410:CDS
AGGTCCTTCTGGCTTGCGCT+TGG 0.474164 tig0022876:-25069 None:intergenic
TTTATGTATGTATGAACCTA+GGG 0.474284 tig0022876:-24037 None:intergenic
GATTAAGAATGATCAACTTA+AGG 0.476396 tig0022876:+25013 Msa1402410:CDS
AGGTGAGGAACAAGATTCTC+AGG 0.477687 tig0022876:+24922 Msa1402410:intron
TGGAGCATGTTATGCCATCT+AGG 0.488269 tig0022876:+24964 Msa1402410:CDS
TTCGTGTTGCTTGTGGGTCG+TGG 0.496840 tig0022876:+25357 Msa1402410:three_prime_UTR
TATGTCGATATCAGGGTTAA+CGG 0.499321 tig0022876:+24249 Msa1402410:CDS
GATGTGGTTAATGATCTTAA+GGG 0.514482 tig0022876:+25149 Msa1402410:CDS
ATTCACTGTCTGATACGAAA+TGG 0.515343 tig0022876:-24076 None:intergenic
CATGGTTGAAGGAGCTACAT+TGG 0.515633 tig0022876:+25103 Msa1402410:CDS
CAAACGTGCTATTGGTGTTG+AGG 0.526075 tig0022876:+24338 Msa1402410:CDS
TCAACTTAAGGCTGAGGCTA+AGG 0.526123 tig0022876:+25025 Msa1402410:CDS
AGAAAGATGCCGGCTGGTCA+TGG 0.530667 tig0022876:+24129 Msa1402410:exon
GCACCGAGGAGTTCCGTTTG+AGG 0.535387 tig0022876:+24988 Msa1402410:CDS
AAGTTCTACCATGGGCGTAC+CGG 0.536603 tig0022876:+24297 Msa1402410:CDS
GAATGATCAACTTAAGGCTG+AGG 0.542856 tig0022876:+25019 Msa1402410:CDS
GTTAACGGTGCCGTTCATAA+GGG 0.556218 tig0022876:+24264 Msa1402410:CDS
TTTCGTATCAGACAGTGAAT+CGG 0.557124 tig0022876:+24079 Msa1402410:five_prime_UTR
GATCTCAAACCATGACCAGC+CGG 0.563821 tig0022876:-24138 None:intergenic
AAGGCTGAGGCTAAGGCAAA+GGG 0.569105 tig0022876:+25032 Msa1402410:CDS
TAAGGCTGAGGCTAAGGCAA+AGG 0.574426 tig0022876:+25031 Msa1402410:CDS
CGCCGATGTGATAGGTTCTG+AGG 0.582425 tig0022876:-24224 None:intergenic
TAACATGCTCCACTCGAACA+TGG 0.582558 tig0022876:-24953 None:intergenic
AGGAGAAGAAAGATGCCGGC+TGG 0.584433 tig0022876:+24123 Msa1402410:exon
CTTCCTCAAACGGAACTCCT+CGG 0.593955 tig0022876:-24991 None:intergenic
AGCACCAAGCGCAAGCCAGA+AGG 0.595144 tig0022876:+25065 Msa1402410:CDS
TACTCCAATTCCATATGATG+TGG 0.596403 tig0022876:+25133 Msa1402410:CDS
TAGAAGGAGAAGAAAGATGC+CGG 0.597264 tig0022876:+24119 Msa1402410:exon
TCTCGTCCATTCAGGAAGAA+GGG 0.597777 tig0022876:+24180 Msa1402410:CDS
GTGATAGGTTCTGAGGTAAG+TGG 0.598261 tig0022876:-24217 None:intergenic
GGTAACGTTCCAGACACGAC+CGG 0.599652 tig0022876:-24316 None:intergenic
GGTTCTGAGGTAAGTGGCGA+GGG 0.600826 tig0022876:-24211 None:intergenic
AAAGCCCAATAAGAAACCCT+AGG 0.603225 tig0022876:+24021 Msa1402410:five_prime_UTR
CGCCCATGGTAGAACTTGTG+TGG 0.607884 tig0022876:-24291 None:intergenic
GCTCCTTCAACCATGAAACC+AGG 0.609967 tig0022876:-25095 None:intergenic
TGCCACACAAGTTCTACCAT+GGG 0.615951 tig0022876:+24289 Msa1402410:CDS
AGGTTCTGAGGTAAGTGGCG+AGG 0.615968 tig0022876:-24212 None:intergenic
AGACACGACCGGTACGCCCA+TGG 0.619384 tig0022876:-24305 None:intergenic
ATGCCACACAAGTTCTACCA+TGG 0.619578 tig0022876:+24288 Msa1402410:CDS
CGGCGATTATGTCGATATCA+GGG 0.624782 tig0022876:+24242 Msa1402410:CDS
AAGCCAGAAGGACCTAAACC+TGG 0.625100 tig0022876:+25077 Msa1402410:CDS
GTGGTTAATGATCTTAAGGG+AGG 0.627017 tig0022876:+25152 Msa1402410:CDS
GTGTGGCATACCCTTATGAA+CGG 0.627528 tig0022876:-24274 None:intergenic
GAAGAGAATCCATGTTCGAG+TGG 0.642302 tig0022876:+24944 Msa1402410:CDS
ACTACCTTTAAGTGAAATCG+AGG 0.644447 tig0022876:+25243 Msa1402410:intron
TACCTCAGAACCTATCACAT+CGG 0.680253 tig0022876:+24222 Msa1402410:CDS
TATGCCATCTAGGTGCACCG+AGG 0.784253 tig0022876:+24974 Msa1402410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTGATTTATTGTATTTT+AGG + tig0022876:24480-24499 Msa1402410:intron 10.0%
!!! TATTGATTTATTGTATTTTA+GGG + tig0022876:24481-24500 Msa1402410:intron 10.0%
!!! ATTATATCTTTTTAATAATG+TGG + tig0022876:25330-25349 Msa1402410:three_prime_UTR 10.0%
!!! TTATTGATTTATTGTATTTT+AGG + tig0022876:24480-24499 Msa1402410:intron 10.0%
!!! TATTGATTTATTGTATTTTA+GGG + tig0022876:24481-24500 Msa1402410:intron 10.0%
!!! ATTATATCTTTTTAATAATG+TGG + tig0022876:25330-25349 Msa1402410:three_prime_UTR 10.0%
!! ATATAATTACATTGATCAAA+TGG - tig0022876:24846-24865 None:intergenic 15.0%
!!! ATTATATGTTGTTATTTATC+TGG + tig0022876:24859-24878 Msa1402410:intron 15.0%
!! ATATAATTACATTGATCAAA+TGG - tig0022876:24846-24865 None:intergenic 15.0%
!!! ATTATATGTTGTTATTTATC+TGG + tig0022876:24859-24878 Msa1402410:intron 15.0%
!!! GAATTTTGTAAATTTACTTG+TGG + tig0022876:24683-24702 Msa1402410:intron 20.0%
!!! TATTGAAATATTGCTATTTG+TGG + tig0022876:24896-24915 Msa1402410:intron 20.0%
!!! GAATTTTGTAAATTTACTTG+TGG + tig0022876:24683-24702 Msa1402410:intron 20.0%
!!! TATTGAAATATTGCTATTTG+TGG + tig0022876:24896-24915 Msa1402410:intron 20.0%
!!! TCTATTTGCTTTTTGTCATT+GGG - tig0022876:24001-24020 None:intergenic 25.0%
!!! TTCTATTTGCTTTTTGTCAT+TGG - tig0022876:24002-24021 None:intergenic 25.0%
! TTTATGTATGTATGAACCTA+GGG - tig0022876:24040-24059 None:intergenic 25.0%
!! TTTTATGTATGTATGAACCT+AGG - tig0022876:24041-24060 None:intergenic 25.0%
! AAAACCACAGAACTAAAATT+CGG - tig0022876:24400-24419 None:intergenic 25.0%
!! TGTGTGCTTTAATTACATTT+TGG + tig0022876:24526-24545 Msa1402410:intron 25.0%
! GATTAAGAATGATCAACTTA+AGG + tig0022876:25013-25032 Msa1402410:CDS 25.0%
! AAGACAGTCTAAAAAACATT+AGG - tig0022876:25198-25217 None:intergenic 25.0%
!!! TTAGACTGTCTTATTTTGTT+TGG + tig0022876:25206-25225 Msa1402410:three_prime_UTR 25.0%
!!! TCTATTTGCTTTTTGTCATT+GGG - tig0022876:24001-24020 None:intergenic 25.0%
!!! TTCTATTTGCTTTTTGTCAT+TGG - tig0022876:24002-24021 None:intergenic 25.0%
! TTTATGTATGTATGAACCTA+GGG - tig0022876:24040-24059 None:intergenic 25.0%
!! TTTTATGTATGTATGAACCT+AGG - tig0022876:24041-24060 None:intergenic 25.0%
! AAAACCACAGAACTAAAATT+CGG - tig0022876:24400-24419 None:intergenic 25.0%
!! TGTGTGCTTTAATTACATTT+TGG + tig0022876:24526-24545 Msa1402410:intron 25.0%
! GATTAAGAATGATCAACTTA+AGG + tig0022876:25013-25032 Msa1402410:CDS 25.0%
! AAGACAGTCTAAAAAACATT+AGG - tig0022876:25198-25217 None:intergenic 25.0%
!!! TTAGACTGTCTTATTTTGTT+TGG + tig0022876:25206-25225 Msa1402410:three_prime_UTR 25.0%
!!! ATTTCCGAATTTTAGTTCTG+TGG + tig0022876:24393-24412 Msa1402410:intron 30.0%
! AGGGTTTATGATTTTCTATG+CGG + tig0022876:24500-24519 Msa1402410:intron 30.0%
!! AATATTGCTATTTGTGGTTC+AGG + tig0022876:24902-24921 Msa1402410:intron 30.0%
CATTCTTAATCTTCCTCAAA+CGG - tig0022876:25004-25023 None:intergenic 30.0%
TTAACCACATCATATGGAAT+TGG - tig0022876:25140-25159 None:intergenic 30.0%
AGATCATTAACCACATCATA+TGG - tig0022876:25146-25165 None:intergenic 30.0%
TGATGTGGTTAATGATCTTA+AGG + tig0022876:25148-25167 Msa1402410:CDS 30.0%
GATGTGGTTAATGATCTTAA+GGG + tig0022876:25149-25168 Msa1402410:CDS 30.0%
!!! ATTTCCGAATTTTAGTTCTG+TGG + tig0022876:24393-24412 Msa1402410:intron 30.0%
! AGGGTTTATGATTTTCTATG+CGG + tig0022876:24500-24519 Msa1402410:intron 30.0%
!! AATATTGCTATTTGTGGTTC+AGG + tig0022876:24902-24921 Msa1402410:intron 30.0%
CATTCTTAATCTTCCTCAAA+CGG - tig0022876:25004-25023 None:intergenic 30.0%
TTAACCACATCATATGGAAT+TGG - tig0022876:25140-25159 None:intergenic 30.0%
AGATCATTAACCACATCATA+TGG - tig0022876:25146-25165 None:intergenic 30.0%
TGATGTGGTTAATGATCTTA+AGG + tig0022876:25148-25167 Msa1402410:CDS 30.0%
GATGTGGTTAATGATCTTAA+GGG + tig0022876:25149-25168 Msa1402410:CDS 30.0%
TGAACCTAGGGTTTCTTATT+GGG - tig0022876:24028-24047 None:intergenic 35.0%
ATGAACCTAGGGTTTCTTAT+TGG - tig0022876:24029-24048 None:intergenic 35.0%
ATTCACTGTCTGATACGAAA+TGG - tig0022876:24079-24098 None:intergenic 35.0%
TTTCGTATCAGACAGTGAAT+CGG + tig0022876:24079-24098 Msa1402410:five_prime_UTR 35.0%
TATGTCGATATCAGGGTTAA+CGG + tig0022876:24249-24268 Msa1402410:CDS 35.0%
TACTCCAATTCCATATGATG+TGG + tig0022876:25133-25152 Msa1402410:CDS 35.0%
ACTACCTTTAAGTGAAATCG+AGG + tig0022876:25243-25262 Msa1402410:intron 35.0%
TGAACCTAGGGTTTCTTATT+GGG - tig0022876:24028-24047 None:intergenic 35.0%
ATGAACCTAGGGTTTCTTAT+TGG - tig0022876:24029-24048 None:intergenic 35.0%
ATTCACTGTCTGATACGAAA+TGG - tig0022876:24079-24098 None:intergenic 35.0%
TTTCGTATCAGACAGTGAAT+CGG + tig0022876:24079-24098 Msa1402410:five_prime_UTR 35.0%
TATGTCGATATCAGGGTTAA+CGG + tig0022876:24249-24268 Msa1402410:CDS 35.0%
TACTCCAATTCCATATGATG+TGG + tig0022876:25133-25152 Msa1402410:CDS 35.0%
ACTACCTTTAAGTGAAATCG+AGG + tig0022876:25243-25262 Msa1402410:intron 35.0%
!!! TTGCTTTTTGTCATTGGGCT+GGG - tig0022876:23996-24015 None:intergenic 40.0%
!!! TTTGCTTTTTGTCATTGGGC+TGG - tig0022876:23997-24016 None:intergenic 40.0%
AAAGCCCAATAAGAAACCCT+AGG + tig0022876:24021-24040 Msa1402410:five_prime_UTR 40.0%
GCATCTTTCTTCTCCTTCTA+GGG - tig0022876:24119-24138 None:intergenic 40.0%
TAGAAGGAGAAGAAAGATGC+CGG + tig0022876:24119-24138 Msa1402410:exon 40.0%
ATTGTACCCTTCTTCCTGAA+TGG - tig0022876:24189-24208 None:intergenic 40.0%
TACCTCAGAACCTATCACAT+CGG + tig0022876:24222-24241 Msa1402410:CDS 40.0%
AACGTTACCAAACGTGCTAT+TGG + tig0022876:24330-24349 Msa1402410:CDS 40.0%
GAATGATCAACTTAAGGCTG+AGG + tig0022876:25019-25038 Msa1402410:CDS 40.0%
TCAACCATGAAACCAGGTTT+AGG - tig0022876:25092-25111 None:intergenic 40.0%
AAACCTGGTTTCATGGTTGA+AGG + tig0022876:25092-25111 Msa1402410:CDS 40.0%
GTGGTTAATGATCTTAAGGG+AGG + tig0022876:25152-25171 Msa1402410:CDS 40.0%
GACTCCTCGATTTCACTTAA+AGG - tig0022876:25250-25269 None:intergenic 40.0%
!! TGGTTTATTCGTGTTGCTTG+TGG + tig0022876:25350-25369 Msa1402410:three_prime_UTR 40.0%
!! GGTTTATTCGTGTTGCTTGT+GGG + tig0022876:25351-25370 Msa1402410:three_prime_UTR 40.0%
!!! TTGCTTTTTGTCATTGGGCT+GGG - tig0022876:23996-24015 None:intergenic 40.0%
!!! TTTGCTTTTTGTCATTGGGC+TGG - tig0022876:23997-24016 None:intergenic 40.0%
AAAGCCCAATAAGAAACCCT+AGG + tig0022876:24021-24040 Msa1402410:five_prime_UTR 40.0%
GCATCTTTCTTCTCCTTCTA+GGG - tig0022876:24119-24138 None:intergenic 40.0%
TAGAAGGAGAAGAAAGATGC+CGG + tig0022876:24119-24138 Msa1402410:exon 40.0%
ATTGTACCCTTCTTCCTGAA+TGG - tig0022876:24189-24208 None:intergenic 40.0%
TACCTCAGAACCTATCACAT+CGG + tig0022876:24222-24241 Msa1402410:CDS 40.0%
AACGTTACCAAACGTGCTAT+TGG + tig0022876:24330-24349 Msa1402410:CDS 40.0%
GAATGATCAACTTAAGGCTG+AGG + tig0022876:25019-25038 Msa1402410:CDS 40.0%
TCAACCATGAAACCAGGTTT+AGG - tig0022876:25092-25111 None:intergenic 40.0%
AAACCTGGTTTCATGGTTGA+AGG + tig0022876:25092-25111 Msa1402410:CDS 40.0%
GTGGTTAATGATCTTAAGGG+AGG + tig0022876:25152-25171 Msa1402410:CDS 40.0%
GACTCCTCGATTTCACTTAA+AGG - tig0022876:25250-25269 None:intergenic 40.0%
!! TGGTTTATTCGTGTTGCTTG+TGG + tig0022876:25350-25369 Msa1402410:three_prime_UTR 40.0%
!! GGTTTATTCGTGTTGCTTGT+GGG + tig0022876:25351-25370 Msa1402410:three_prime_UTR 40.0%
GGCATCTTTCTTCTCCTTCT+AGG - tig0022876:24120-24139 None:intergenic 45.0%
ACTCTTTCTCTCGTCCATTC+AGG + tig0022876:24172-24191 Msa1402410:CDS 45.0%
TCTCGTCCATTCAGGAAGAA+GGG + tig0022876:24180-24199 Msa1402410:CDS 45.0%
!! GTGATAGGTTCTGAGGTAAG+TGG - tig0022876:24220-24239 None:intergenic 45.0%
GACATAATCGCCGATGTGAT+AGG - tig0022876:24235-24254 None:intergenic 45.0%
TCGGCGATTATGTCGATATC+AGG + tig0022876:24241-24260 Msa1402410:CDS 45.0%
CGGCGATTATGTCGATATCA+GGG + tig0022876:24242-24261 Msa1402410:CDS 45.0%
!! GTTAACGGTGCCGTTCATAA+GGG + tig0022876:24264-24283 Msa1402410:CDS 45.0%
GTGTGGCATACCCTTATGAA+CGG - tig0022876:24277-24296 None:intergenic 45.0%
ATGCCACACAAGTTCTACCA+TGG + tig0022876:24288-24307 Msa1402410:CDS 45.0%
TGCCACACAAGTTCTACCAT+GGG + tig0022876:24289-24308 Msa1402410:CDS 45.0%
CTCAACACCAATAGCACGTT+TGG - tig0022876:24340-24359 None:intergenic 45.0%
!! CAAACGTGCTATTGGTGTTG+AGG + tig0022876:24338-24357 Msa1402410:CDS 45.0%
CAAATGGATGCTTCATGCAC+AGG - tig0022876:24830-24849 None:intergenic 45.0%
! TGCTATTTGTGGTTCAGGTG+AGG + tig0022876:24907-24926 Msa1402410:intron 45.0%
AGGTGAGGAACAAGATTCTC+AGG + tig0022876:24922-24941 Msa1402410:intron 45.0%
GAAGAGAATCCATGTTCGAG+TGG + tig0022876:24944-24963 Msa1402410:CDS 45.0%
TAACATGCTCCACTCGAACA+TGG - tig0022876:24956-24975 None:intergenic 45.0%
TGGAGCATGTTATGCCATCT+AGG + tig0022876:24964-24983 Msa1402410:CDS 45.0%
TCAACTTAAGGCTGAGGCTA+AGG + tig0022876:25025-25044 Msa1402410:CDS 45.0%
AAACCAGGTTTAGGTCCTTC+TGG - tig0022876:25083-25102 None:intergenic 45.0%
AGGACCTAAACCTGGTTTCA+TGG + tig0022876:25085-25104 Msa1402410:CDS 45.0%
CATGGTTGAAGGAGCTACAT+TGG + tig0022876:25103-25122 Msa1402410:CDS 45.0%
GGCATCTTTCTTCTCCTTCT+AGG - tig0022876:24120-24139 None:intergenic 45.0%
ACTCTTTCTCTCGTCCATTC+AGG + tig0022876:24172-24191 Msa1402410:CDS 45.0%
TCTCGTCCATTCAGGAAGAA+GGG + tig0022876:24180-24199 Msa1402410:CDS 45.0%
!! GTGATAGGTTCTGAGGTAAG+TGG - tig0022876:24220-24239 None:intergenic 45.0%
GACATAATCGCCGATGTGAT+AGG - tig0022876:24235-24254 None:intergenic 45.0%
TCGGCGATTATGTCGATATC+AGG + tig0022876:24241-24260 Msa1402410:CDS 45.0%
CGGCGATTATGTCGATATCA+GGG + tig0022876:24242-24261 Msa1402410:CDS 45.0%
!! GTTAACGGTGCCGTTCATAA+GGG + tig0022876:24264-24283 Msa1402410:CDS 45.0%
GTGTGGCATACCCTTATGAA+CGG - tig0022876:24277-24296 None:intergenic 45.0%
ATGCCACACAAGTTCTACCA+TGG + tig0022876:24288-24307 Msa1402410:CDS 45.0%
TGCCACACAAGTTCTACCAT+GGG + tig0022876:24289-24308 Msa1402410:CDS 45.0%
CTCAACACCAATAGCACGTT+TGG - tig0022876:24340-24359 None:intergenic 45.0%
!! CAAACGTGCTATTGGTGTTG+AGG + tig0022876:24338-24357 Msa1402410:CDS 45.0%
CAAATGGATGCTTCATGCAC+AGG - tig0022876:24830-24849 None:intergenic 45.0%
! TGCTATTTGTGGTTCAGGTG+AGG + tig0022876:24907-24926 Msa1402410:intron 45.0%
AGGTGAGGAACAAGATTCTC+AGG + tig0022876:24922-24941 Msa1402410:intron 45.0%
GAAGAGAATCCATGTTCGAG+TGG + tig0022876:24944-24963 Msa1402410:CDS 45.0%
TAACATGCTCCACTCGAACA+TGG - tig0022876:24956-24975 None:intergenic 45.0%
TGGAGCATGTTATGCCATCT+AGG + tig0022876:24964-24983 Msa1402410:CDS 45.0%
TCAACTTAAGGCTGAGGCTA+AGG + tig0022876:25025-25044 Msa1402410:CDS 45.0%
AAACCAGGTTTAGGTCCTTC+TGG - tig0022876:25083-25102 None:intergenic 45.0%
AGGACCTAAACCTGGTTTCA+TGG + tig0022876:25085-25104 Msa1402410:CDS 45.0%
CATGGTTGAAGGAGCTACAT+TGG + tig0022876:25103-25122 Msa1402410:CDS 45.0%
TGCGACGCAAAAACCCTAGA+AGG + tig0022876:24103-24122 Msa1402410:five_prime_UTR 50.0%
GATCTCAAACCATGACCAGC+CGG - tig0022876:24141-24160 None:intergenic 50.0%
CTCTCGTCCATTCAGGAAGA+AGG + tig0022876:24179-24198 Msa1402410:CDS 50.0%
!! GGTTAACGGTGCCGTTCATA+AGG + tig0022876:24263-24282 Msa1402410:CDS 50.0%
AAGTTCTACCATGGGCGTAC+CGG + tig0022876:24297-24316 Msa1402410:CDS 50.0%
CTTCCTCAAACGGAACTCCT+CGG - tig0022876:24994-25013 None:intergenic 50.0%
TAAGGCTGAGGCTAAGGCAA+AGG + tig0022876:25031-25050 Msa1402410:CDS 50.0%
AAGGCTGAGGCTAAGGCAAA+GGG + tig0022876:25032-25051 Msa1402410:CDS 50.0%
AAGCCAGAAGGACCTAAACC+TGG + tig0022876:25077-25096 Msa1402410:CDS 50.0%
GCTCCTTCAACCATGAAACC+AGG - tig0022876:25098-25117 None:intergenic 50.0%
TGCGACGCAAAAACCCTAGA+AGG + tig0022876:24103-24122 Msa1402410:five_prime_UTR 50.0%
GATCTCAAACCATGACCAGC+CGG - tig0022876:24141-24160 None:intergenic 50.0%
CTCTCGTCCATTCAGGAAGA+AGG + tig0022876:24179-24198 Msa1402410:CDS 50.0%
!! GGTTAACGGTGCCGTTCATA+AGG + tig0022876:24263-24282 Msa1402410:CDS 50.0%
AAGTTCTACCATGGGCGTAC+CGG + tig0022876:24297-24316 Msa1402410:CDS 50.0%
CTTCCTCAAACGGAACTCCT+CGG - tig0022876:24994-25013 None:intergenic 50.0%
TAAGGCTGAGGCTAAGGCAA+AGG + tig0022876:25031-25050 Msa1402410:CDS 50.0%
AAGGCTGAGGCTAAGGCAAA+GGG + tig0022876:25032-25051 Msa1402410:CDS 50.0%
AAGCCAGAAGGACCTAAACC+TGG + tig0022876:25077-25096 Msa1402410:CDS 50.0%
GCTCCTTCAACCATGAAACC+AGG - tig0022876:25098-25117 None:intergenic 50.0%
AGGAGAAGAAAGATGCCGGC+TGG + tig0022876:24123-24142 Msa1402410:exon 55.0%
! AGAAAGATGCCGGCTGGTCA+TGG + tig0022876:24129-24148 Msa1402410:exon 55.0%
!! GGTTCTGAGGTAAGTGGCGA+GGG - tig0022876:24214-24233 None:intergenic 55.0%
!! AGGTTCTGAGGTAAGTGGCG+AGG - tig0022876:24215-24234 None:intergenic 55.0%
CGCCGATGTGATAGGTTCTG+AGG - tig0022876:24227-24246 None:intergenic 55.0%
CGCCCATGGTAGAACTTGTG+TGG - tig0022876:24294-24313 None:intergenic 55.0%
GGTAACGTTCCAGACACGAC+CGG - tig0022876:24319-24338 None:intergenic 55.0%
TATGCCATCTAGGTGCACCG+AGG + tig0022876:24974-24993 Msa1402410:CDS 55.0%
! AACTCCTCGGTGCACCTAGA+TGG - tig0022876:24981-25000 None:intergenic 55.0%
!! TTCGTGTTGCTTGTGGGTCG+TGG + tig0022876:25357-25376 Msa1402410:three_prime_UTR 55.0%
AGGAGAAGAAAGATGCCGGC+TGG + tig0022876:24123-24142 Msa1402410:exon 55.0%
! AGAAAGATGCCGGCTGGTCA+TGG + tig0022876:24129-24148 Msa1402410:exon 55.0%
!! GGTTCTGAGGTAAGTGGCGA+GGG - tig0022876:24214-24233 None:intergenic 55.0%
!! AGGTTCTGAGGTAAGTGGCG+AGG - tig0022876:24215-24234 None:intergenic 55.0%
CGCCGATGTGATAGGTTCTG+AGG - tig0022876:24227-24246 None:intergenic 55.0%
CGCCCATGGTAGAACTTGTG+TGG - tig0022876:24294-24313 None:intergenic 55.0%
GGTAACGTTCCAGACACGAC+CGG - tig0022876:24319-24338 None:intergenic 55.0%
TATGCCATCTAGGTGCACCG+AGG + tig0022876:24974-24993 Msa1402410:CDS 55.0%
! AACTCCTCGGTGCACCTAGA+TGG - tig0022876:24981-25000 None:intergenic 55.0%
!! TTCGTGTTGCTTGTGGGTCG+TGG + tig0022876:25357-25376 Msa1402410:three_prime_UTR 55.0%
GCACCGAGGAGTTCCGTTTG+AGG + tig0022876:24988-25007 Msa1402410:CDS 60.0%
AGCACCAAGCGCAAGCCAGA+AGG + tig0022876:25065-25084 Msa1402410:CDS 60.0%
!! AGGTCCTTCTGGCTTGCGCT+TGG - tig0022876:25072-25091 None:intergenic 60.0%
GCACCGAGGAGTTCCGTTTG+AGG + tig0022876:24988-25007 Msa1402410:CDS 60.0%
AGCACCAAGCGCAAGCCAGA+AGG + tig0022876:25065-25084 Msa1402410:CDS 60.0%
!! AGGTCCTTCTGGCTTGCGCT+TGG - tig0022876:25072-25091 None:intergenic 60.0%
AGACACGACCGGTACGCCCA+TGG - tig0022876:24308-24327 None:intergenic 65.0%
! ATGGGCGTACCGGTCGTGTC+TGG + tig0022876:24307-24326 Msa1402410:CDS 65.0%
AGACACGACCGGTACGCCCA+TGG - tig0022876:24308-24327 None:intergenic 65.0%
! ATGGGCGTACCGGTCGTGTC+TGG + tig0022876:24307-24326 Msa1402410:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0022876 gene 23979 25389 23979 ID=Msa1402410;Name=Msa1402410
tig0022876 mRNA 23979 25389 23979 ID=Msa1402410-mRNA-1;Parent=Msa1402410;Name=Msa1402410-mRNA-1;_AED=0.02;_eAED=0.02;_QI=156|1|1|1|0.5|0.33|3|188|164
tig0022876 exon 23979 24371 23979 ID=Msa1402410-mRNA-1:exon:18896;Parent=Msa1402410-mRNA-1
tig0022876 exon 24924 25234 24924 ID=Msa1402410-mRNA-1:exon:18897;Parent=Msa1402410-mRNA-1
tig0022876 exon 25255 25389 25255 ID=Msa1402410-mRNA-1:exon:18898;Parent=Msa1402410-mRNA-1
tig0022876 five_prime_UTR 23979 24134 23979 ID=Msa1402410-mRNA-1:five_prime_utr;Parent=Msa1402410-mRNA-1
tig0022876 CDS 24135 24371 24135 ID=Msa1402410-mRNA-1:cds;Parent=Msa1402410-mRNA-1
tig0022876 CDS 24924 25181 24924 ID=Msa1402410-mRNA-1:cds;Parent=Msa1402410-mRNA-1
tig0022876 three_prime_UTR 25182 25234 25182 ID=Msa1402410-mRNA-1:three_prime_utr;Parent=Msa1402410-mRNA-1
tig0022876 three_prime_UTR 25255 25389 25255 ID=Msa1402410-mRNA-1:three_prime_utr;Parent=Msa1402410-mRNA-1
Gene Sequence

>Msa1402410

ATGCCGGCTGGTCATGGTTTGAGATCTCGCACGAGAGACTCTTTCTCTCGTCCATTCAGGAAGAAGGGTACAATCGCCCTCGCCACTTACCTCAGAACCTATCACATCGGCGATTATGTCGATATCAGGGTTAACGGTGCCGTTCATAAGGGTATGCCACACAAGTTCTACCATGGGCGTACCGGTCGTGTCTGGAACGTTACCAAACGTGCTATTGGTGTTGAGGTTAACAAACAAGTGAGGAACAAGATTCTCAGGAAGAGAATCCATGTTCGAGTGGAGCATGTTATGCCATCTAGGTGCACCGAGGAGTTCCGTTTGAGGAAGATTAAGAATGATCAACTTAAGGCTGAGGCTAAGGCAAAGGGTGAAGTCATTAGCACCAAGCGCAAGCCAGAAGGACCTAAACCTGGTTTCATGGTTGAAGGAGCTACATTGGAAACTGTTACTCCAATTCCATATGATGTGGTTAATGATCTTAAGGGAGGATACTAG
Protein sequence

>Msa1402410

MPAGHGLRSRTRDSFSRPFRKKGTIALATYLRTYHIGDYVDIRVNGAVHKGMPHKFYHGRTGRVWNVTKRAIGVEVNKQVRNKILRKRIHVRVEHVMPSRCTEEFRLRKIKNDQLKAEAKAKGEVISTKRKPEGPKPGFMVEGATLETVTPIPYDVVNDLKGGY*