AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0191550


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0191550 MtrunA17_Chr2g0281331 100.000 146 0 0 1 146 1 146 8.25e-104 293
Msa0191550 MtrunA17_Chr5g0401191 95.205 146 7 0 1 146 1 146 1.61e-90 259
Msa0191550 MtrunA17_Chr5g0447011 91.096 146 13 0 1 146 1 146 3.37e-87 251
Msa0191550 MtrunA17_Chr8g0370201 56.977 86 10 1 30 115 1 59 2.09e-23 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0191550 AT5G67510.1 88.356 146 17 0 1 146 1 146 1.04e-92 265
Msa0191550 AT3G49910.1 85.616 146 21 0 1 146 1 146 1.23e-91 262

Find 50 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATGCAGACCGTTGATT+AGG 0.363868 2_1:-7168052 None:intergenic
CATTGAACGGACGTTGTACT+TGG 0.377101 2_1:+7168353 None:intergenic
TCGCAAAGACGACGAAGTTC+AGG 0.379749 2_1:-7168325 Msa0191550:CDS
TTAGTGATAACAACCTTTGA+AGG 0.395167 2_1:+7168174 None:intergenic
ACTTCGTCGTCTTTGCGAAC+AGG 0.416424 2_1:+7168330 None:intergenic
ATGGATCTACCGTTAATGTT+GGG 0.416486 2_1:-7168203 Msa0191550:CDS
GGGTCGTGCTGCTGCTGATA+AGG 0.420559 2_1:-7168103 Msa0191550:CDS
ACGGCTCTTCCTACGGCTGC+TGG 0.422834 2_1:+7168449 None:intergenic
AAGACACGGCGGACGCTTGA+TGG 0.437229 2_1:+7168408 None:intergenic
CACTAAGCTCAGATTGGATA+AGG 0.438891 2_1:-7168157 Msa0191550:CDS
CCTGAGTAACCTTACCTTCA+CGG 0.446679 2_1:+7168280 None:intergenic
AGGATTAACCCCAACATTAA+CGG 0.448396 2_1:+7168194 None:intergenic
TGTTATCACTAAGCTCAGAT+TGG 0.466490 2_1:-7168163 Msa0191550:CDS
GACGAAGTTCAGGTCGTTCG+TGG 0.488796 2_1:-7168315 Msa0191550:CDS
ATTCAAAGGCCGTGAAGGTA+AGG 0.498862 2_1:-7168289 Msa0191550:CDS
CTCAGGTTTACCGTCGCAAG+TGG 0.512762 2_1:-7168263 Msa0191550:CDS
TGTTGGGGTTAATCCTTCAA+AGG 0.524932 2_1:-7168187 Msa0191550:CDS
GCTACTTGATCGTAAGGCTA+AGG 0.528270 2_1:-7168124 Msa0191550:CDS
AATGGATCTACCGTTAATGT+TGG 0.529601 2_1:-7168204 Msa0191550:CDS
CTACGGCTGCTGGAAACTCG+TGG 0.530535 2_1:+7168459 None:intergenic
TGCTGCTGCTGATAAGGAGA+AGG 0.537077 2_1:-7168097 Msa0191550:CDS
GAGCCGTAAGGCTCATTTCA+CGG 0.545137 2_1:-7168433 Msa0191550:CDS
ATCACAAGGGAGAAGGTGAA+TGG 0.545267 2_1:-7168222 Msa0191550:CDS
GAGCCTTACGGCTCTTCCTA+CGG 0.547173 2_1:+7168442 None:intergenic
CTACTTGATCGTAAGGCTAA+GGG 0.553374 2_1:-7168123 Msa0191550:CDS
GTCGTTCGTGGAACATTCAA+AGG 0.554383 2_1:-7168303 Msa0191550:CDS
TAAATCGCTACTTGATCGTA+AGG 0.555125 2_1:-7168130 Msa0191550:CDS
GGGTACTAAGTTTGCTCCTG+AGG 0.563912 2_1:-7168076 Msa0191550:CDS
TGGATCTACCGTTAATGTTG+GGG 0.564613 2_1:-7168202 Msa0191550:CDS
AACGGACGTTGTACTTGGAT+CGG 0.564652 2_1:+7168358 None:intergenic
CCGTGAAGGTAAGGTTACTC+AGG 0.566699 2_1:-7168280 Msa0191550:CDS
CTTTGCGAACAGGCATTGAA+CGG 0.570326 2_1:+7168340 None:intergenic
GTTGTACTTGGATCGGAGAT+CGG 0.572814 2_1:+7168365 None:intergenic
ACGGTCTGCATAATATCCTC+AGG 0.575139 2_1:+7168060 None:intergenic
GCGCCGTGAAATGAGCCTTA+CGG 0.575259 2_1:+7168430 None:intergenic
CGAACGCATCACAAGGGAGA+AGG 0.580140 2_1:-7168229 Msa0191550:CDS
GATCGGAGATCGGCGGAGAG+TGG 0.584970 2_1:+7168375 None:intergenic
TACGGCTGCTGGAAACTCGT+GGG 0.586325 2_1:+7168460 None:intergenic
TTCACATCGAACGCATCACA+AGG 0.588472 2_1:-7168236 Msa0191550:CDS
CAGCCGTAGGAAGAGCCGTA+AGG 0.591177 2_1:-7168445 Msa0191550:CDS
GGAACATTCAAAGGCCGTGA+AGG 0.606062 2_1:-7168294 Msa0191550:CDS
GATTCAAGAATCCTAATCAA+CGG 0.625900 2_1:+7168041 None:intergenic
GCTGCTGCTGATAAGGAGAA+GGG 0.634378 2_1:-7168096 Msa0191550:CDS
TGTGAATCACCCACTTGCGA+CGG 0.658219 2_1:+7168253 None:intergenic
TCAGGTTTACCGTCGCAAGT+GGG 0.670860 2_1:-7168262 Msa0191550:CDS
CACGAGTTTCCAGCAGCCGT+AGG 0.686122 2_1:-7168458 Msa0191550:CDS
GTGGCGCGCTCATTAAGACA+CGG 0.705562 2_1:+7168394 None:intergenic
GTACTTGGATCGGAGATCGG+CGG 0.709980 2_1:+7168368 None:intergenic
TCACATCGAACGCATCACAA+GGG 0.710980 2_1:-7168235 Msa0191550:CDS
GCGCGCTCATTAAGACACGG+CGG 0.823768 2_1:+7168397 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTAGTGATAACAACCTTTGA+AGG + chr2_1:7168353-7168372 None:intergenic 30.0%
AATGGATCTACCGTTAATGT+TGG - chr2_1:7168320-7168339 Msa0191550:CDS 35.0%
ATGGATCTACCGTTAATGTT+GGG - chr2_1:7168321-7168340 Msa0191550:CDS 35.0%
AGGATTAACCCCAACATTAA+CGG + chr2_1:7168333-7168352 None:intergenic 35.0%
TGTTATCACTAAGCTCAGAT+TGG - chr2_1:7168361-7168380 Msa0191550:CDS 35.0%
TAAATCGCTACTTGATCGTA+AGG - chr2_1:7168394-7168413 Msa0191550:CDS 35.0%
TGGATCTACCGTTAATGTTG+GGG - chr2_1:7168322-7168341 Msa0191550:CDS 40.0%
TGTTGGGGTTAATCCTTCAA+AGG - chr2_1:7168337-7168356 Msa0191550:CDS 40.0%
CACTAAGCTCAGATTGGATA+AGG - chr2_1:7168367-7168386 Msa0191550:CDS 40.0%
CTACTTGATCGTAAGGCTAA+GGG - chr2_1:7168401-7168420 Msa0191550:CDS 40.0%
GTTGTACTTGGATCGGAGAT+CGG + chr2_1:7168162-7168181 None:intergenic 45.0%
AACGGACGTTGTACTTGGAT+CGG + chr2_1:7168169-7168188 None:intergenic 45.0%
CATTGAACGGACGTTGTACT+TGG + chr2_1:7168174-7168193 None:intergenic 45.0%
!! CTTTGCGAACAGGCATTGAA+CGG + chr2_1:7168187-7168206 None:intergenic 45.0%
GTCGTTCGTGGAACATTCAA+AGG - chr2_1:7168221-7168240 Msa0191550:CDS 45.0%
ATTCAAAGGCCGTGAAGGTA+AGG - chr2_1:7168235-7168254 Msa0191550:CDS 45.0%
CCTGAGTAACCTTACCTTCA+CGG + chr2_1:7168247-7168266 None:intergenic 45.0%
TTCACATCGAACGCATCACA+AGG - chr2_1:7168288-7168307 Msa0191550:CDS 45.0%
TCACATCGAACGCATCACAA+GGG - chr2_1:7168289-7168308 Msa0191550:CDS 45.0%
ATCACAAGGGAGAAGGTGAA+TGG - chr2_1:7168302-7168321 Msa0191550:CDS 45.0%
GCTACTTGATCGTAAGGCTA+AGG - chr2_1:7168400-7168419 Msa0191550:CDS 45.0%
ACGGTCTGCATAATATCCTC+AGG + chr2_1:7168467-7168486 None:intergenic 45.0%
GAGCCGTAAGGCTCATTTCA+CGG - chr2_1:7168091-7168110 Msa0191550:CDS 50.0%
ACTTCGTCGTCTTTGCGAAC+AGG + chr2_1:7168197-7168216 None:intergenic 50.0%
TCGCAAAGACGACGAAGTTC+AGG - chr2_1:7168199-7168218 Msa0191550:CDS 50.0%
GGAACATTCAAAGGCCGTGA+AGG - chr2_1:7168230-7168249 Msa0191550:CDS 50.0%
CCGTGAAGGTAAGGTTACTC+AGG - chr2_1:7168244-7168263 Msa0191550:CDS 50.0%
TCAGGTTTACCGTCGCAAGT+GGG - chr2_1:7168262-7168281 Msa0191550:CDS 50.0%
TGTGAATCACCCACTTGCGA+CGG + chr2_1:7168274-7168293 None:intergenic 50.0%
TGCTGCTGCTGATAAGGAGA+AGG - chr2_1:7168427-7168446 Msa0191550:CDS 50.0%
GCTGCTGCTGATAAGGAGAA+GGG - chr2_1:7168428-7168447 Msa0191550:CDS 50.0%
! GGGTACTAAGTTTGCTCCTG+AGG - chr2_1:7168448-7168467 Msa0191550:CDS 50.0%
TACGGCTGCTGGAAACTCGT+GGG + chr2_1:7168067-7168086 None:intergenic 55.0%
GAGCCTTACGGCTCTTCCTA+CGG + chr2_1:7168085-7168104 None:intergenic 55.0%
GCGCCGTGAAATGAGCCTTA+CGG + chr2_1:7168097-7168116 None:intergenic 55.0%
GTGGCGCGCTCATTAAGACA+CGG + chr2_1:7168133-7168152 None:intergenic 55.0%
GTACTTGGATCGGAGATCGG+CGG + chr2_1:7168159-7168178 None:intergenic 55.0%
GACGAAGTTCAGGTCGTTCG+TGG - chr2_1:7168209-7168228 Msa0191550:CDS 55.0%
CTCAGGTTTACCGTCGCAAG+TGG - chr2_1:7168261-7168280 Msa0191550:CDS 55.0%
CGAACGCATCACAAGGGAGA+AGG - chr2_1:7168295-7168314 Msa0191550:CDS 55.0%
CTACGGCTGCTGGAAACTCG+TGG + chr2_1:7168068-7168087 None:intergenic 60.0%
CACGAGTTTCCAGCAGCCGT+AGG - chr2_1:7168066-7168085 Msa0191550:CDS 60.0%
CAGCCGTAGGAAGAGCCGTA+AGG - chr2_1:7168079-7168098 Msa0191550:CDS 60.0%
AAGACACGGCGGACGCTTGA+TGG + chr2_1:7168119-7168138 None:intergenic 60.0%
GCGCGCTCATTAAGACACGG+CGG + chr2_1:7168130-7168149 None:intergenic 60.0%
! GGGTCGTGCTGCTGCTGATA+AGG - chr2_1:7168421-7168440 Msa0191550:CDS 60.0%
ACGGCTCTTCCTACGGCTGC+TGG + chr2_1:7168078-7168097 None:intergenic 65.0%
GATCGGAGATCGGCGGAGAG+TGG + chr2_1:7168152-7168171 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 7168053 7168493 7168053 ID=Msa0191550;Name=Msa0191550
chr2_1 mRNA 7168053 7168493 7168053 ID=Msa0191550-mRNA-1;Parent=Msa0191550;Name=Msa0191550-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|146
chr2_1 exon 7168053 7168493 7168053 ID=Msa0191550-mRNA-1:exon:3250;Parent=Msa0191550-mRNA-1
chr2_1 CDS 7168053 7168493 7168053 ID=Msa0191550-mRNA-1:cds;Parent=Msa0191550-mRNA-1
Gene Sequence

>Msa0191550

ATGAAGTTCAACCCACGAGTTTCCAGCAGCCGTAGGAAGAGCCGTAAGGCTCATTTCACGGCGCCATCAAGCGTCCGCCGTGTCTTAATGAGCGCGCCACTCTCCGCCGATCTCCGATCCAAGTACAACGTCCGTTCAATGCCTGTTCGCAAAGACGACGAAGTTCAGGTCGTTCGTGGAACATTCAAAGGCCGTGAAGGTAAGGTTACTCAGGTTTACCGTCGCAAGTGGGTGATTCACATCGAACGCATCACAAGGGAGAAGGTGAATGGATCTACCGTTAATGTTGGGGTTAATCCTTCAAAGGTTGTTATCACTAAGCTCAGATTGGATAAGGATCGTAAATCGCTACTTGATCGTAAGGCTAAGGGTCGTGCTGCTGCTGATAAGGAGAAGGGTACTAAGTTTGCTCCTGAGGATATTATGCAGACCGTTGATTAG

Protein sequence

>Msa0191550

MKFNPRVSSSRRKSRKAHFTAPSSVRRVLMSAPLSADLRSKYNVRSMPVRKDDEVQVVRGTFKGREGKVTQVYRRKWVIHIERITREKVNGSTVNVGVNPSKVVITKLRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQTVD*