AlfalfaGEDB | Msa0191450

M. sativa cultivar zhongmu-4 / Msa0191450

KEGG: K02898
GO: GO:0002181 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0010467 GO:0015934 GO:0016020 GO:0019538 GO:0022613 GO:0022625 GO:0022626 GO:0031090 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0034645 GO:0042254 GO:0042273 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0050896 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0098588 GO:0098805 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0191450	XP_003593461.1	100.000	146	0	0	1	146	1	146	8.96e-100	293

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0191450	sp\|Q9FJX2\|RL262_ARATH	88.356	146	17	0	1	146	1	146	1.02e-91	265

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0191450	G7ILF2	100.000	146	0	0	1	146	1	146	4.28e-100	293

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001760	Msa0191450	0.868290	7.289126e-66	-8.615850e-47
Msa0003330	Msa0191450	0.882625	8.904934e-71	-8.615850e-47
Msa0003360	Msa0191450	0.807595	4.680695e-50	-8.615850e-47
Msa0027590	Msa0191450	0.905120	6.002201e-80	-8.615850e-47
Msa0040220	Msa0191450	0.851296	9.734756e-61	-8.615850e-47
Msa0042480	Msa0191450	0.815930	7.180888e-52	-8.615850e-47
Msa0065110	Msa0191450	0.811969	5.364916e-51	-8.615850e-47
Msa0070840	Msa0191450	0.814074	1.854032e-51	-8.615850e-47
Msa0072570	Msa0191450	0.836247	1.046707e-56	-8.615850e-47
Msa0075350	Msa0191450	0.884809	1.396330e-71	-8.615850e-47
Msa0079590	Msa0191450	0.829085	6.268576e-55	-8.615850e-47
Msa0087910	Msa0191450	0.851559	8.201333e-61	-8.615850e-47
Msa0094210	Msa0191450	0.821798	3.333278e-53	-8.615850e-47
Msa0114570	Msa0191450	0.844889	5.715155e-59	-8.615850e-47
Msa0128330	Msa0191450	0.891771	2.929422e-74	-8.615850e-47
Msa0128750	Msa0191450	0.840651	7.647825e-58	-8.615850e-47
Msa0132460	Msa0191450	0.846465	2.135921e-59	-8.615850e-47
Msa0134580	Msa0191450	0.879636	1.059801e-69	-8.615850e-47
Msa0138890	Msa0191450	0.825332	4.965674e-54	-8.615850e-47
Msa0146580	Msa0191450	0.803044	4.206945e-49	-8.615850e-47
Msa0167860	Msa0191450	0.883636	3.793271e-71	-8.615850e-47
Msa0168320	Msa0191450	0.855586	5.713493e-62	-8.615850e-47
Msa0172930	Msa0191450	0.804063	2.586244e-49	-8.615850e-47
Msa0174580	Msa0191450	0.882105	1.376955e-70	-8.615850e-47
Msa0182050	Msa0191450	0.850253	1.913506e-60	-8.615850e-47
Msa0182100	Msa0191450	0.815794	7.700498e-52	-8.615850e-47
Msa0186740	Msa0191450	0.819836	9.421799e-53	-8.615850e-47
Msa0191450	Msa0191550	0.986072	1.397099e-165	-8.615850e-47
Msa0191450	Msa0192300	0.844835	5.910655e-59	-8.615850e-47
Msa0191450	Msa0192750	0.833638	4.753028e-56	-8.615850e-47
Msa0191450	Msa0194940	0.903615	2.888431e-79	-8.615850e-47
Msa0191450	Msa0210330	0.803502	3.381643e-49	-8.615850e-47
Msa0191450	Msa0210360	0.830027	3.699284e-55	-8.615850e-47
Msa0191450	Msa0216200	0.818755	1.660763e-52	-8.615850e-47
Msa0191450	Msa0222730	0.804856	1.767100e-49	-8.615850e-47
Msa0191450	Msa0233550	0.910177	2.507775e-82	-8.615850e-47
Msa0191450	Msa0235050	0.849995	2.260228e-60	-8.615850e-47
Msa0191450	Msa0239420	0.876425	1.411274e-68	-8.615850e-47
Msa0191450	Msa0277600	0.861870	7.568173e-64	-8.615850e-47
Msa0191450	Msa0281330	0.833612	4.825867e-56	-8.615850e-47
Msa0191450	Msa0281370	0.879353	1.335738e-69	-8.615850e-47
Msa0191450	Msa0297830	0.865096	7.565916e-65	-8.615850e-47
Msa0191450	Msa0301220	0.850625	1.504932e-60	-8.615850e-47
Msa0191450	Msa0301250	0.849288	3.559386e-60	-8.615850e-47
Msa0191450	Msa0312210	0.824646	7.211628e-54	-8.615850e-47
Msa0191450	Msa0313060	0.856571	2.941342e-62	-8.615850e-47
Msa0191450	Msa0313520	0.806657	7.395568e-50	-8.615850e-47
Msa0191450	Msa0315430	0.881685	1.953861e-70	-8.615850e-47
Msa0191450	Msa0321270	0.802617	5.153951e-49	-8.615850e-47
Msa0191450	Msa0334140	0.806498	7.988459e-50	-8.615850e-47
Msa0191450	Msa0335840	0.872655	2.691417e-67	-8.615850e-47
Msa0191450	Msa0340560	0.832036	1.188649e-55	-8.615850e-47
Msa0191450	Msa0369410	0.857000	2.198514e-62	-8.615850e-47
Msa0191450	Msa0371490	0.841606	4.292176e-58	-8.615850e-47
Msa0191450	Msa0377520	0.845429	4.083057e-59	-8.615850e-47
Msa0191450	Msa0382670	0.809769	1.606300e-50	-8.615850e-47
Msa0191450	Msa0382960	0.800494	1.403532e-48	-8.615850e-47
Msa0191450	Msa0426090	0.899861	1.303087e-77	-8.615850e-47
Msa0191450	Msa0426250	0.887602	1.235046e-72	-8.615850e-47
Msa0191450	Msa0436750	0.820010	8.597761e-53	-8.615850e-47
Msa0191450	Msa0438960	0.806538	7.837406e-50	-8.615850e-47
Msa0191450	Msa0463520	0.825172	5.417573e-54	-8.615850e-47
Msa0191450	Msa0471880	0.871149	8.516405e-67	-8.615850e-47
Msa0191450	Msa0472480	0.897328	1.564457e-76	-8.615850e-47
Msa0191450	Msa0473570	0.820259	7.538413e-53	-8.615850e-47
Msa0191450	Msa0475810	0.840024	1.115457e-57	-8.615850e-47
Msa0191450	Msa0481520	0.906244	1.825980e-80	-8.615850e-47
Msa0191450	Msa0481850	0.841709	4.031252e-58	-8.615850e-47
Msa0191450	Msa0484730	0.813846	2.081531e-51	-8.615850e-47
Msa0191450	Msa0511760	0.814190	1.747999e-51	-8.615850e-47
Msa0191450	Msa0517480	0.873300	1.636312e-67	-8.615850e-47
Msa0191450	Msa0529900	0.867078	1.783429e-65	-8.615850e-47
Msa0191450	Msa0540480	0.841004	6.181087e-58	-8.615850e-47
Msa0191450	Msa0547920	0.847070	1.458664e-59	-8.615850e-47
Msa0191450	Msa0550420	0.838661	2.518621e-57	-8.615850e-47
Msa0191450	Msa0573550	0.889031	3.485981e-73	-8.615850e-47
Msa0191450	Msa0574780	0.830582	2.708463e-55	-8.615850e-47
Msa0191450	Msa0579830	0.864462	1.194878e-64	-8.615850e-47
Msa0191450	Msa0582070	0.856310	3.508200e-62	-8.615850e-47
Msa0191450	Msa0584030	0.827752	1.315433e-54	-8.615850e-47
Msa0191450	Msa0591030	0.818757	1.659670e-52	-8.615850e-47
Msa0191450	Msa0591050	0.873042	1.997736e-67	-8.615850e-47
Msa0191450	Msa0596370	0.813157	2.950337e-51	-8.615850e-47
Msa0191450	Msa0597240	0.849843	2.493310e-60	-8.615850e-47
Msa0191450	Msa0602900	0.828064	1.106455e-54	-8.615850e-47
Msa0191450	Msa0605890	0.876212	1.671163e-68	-8.615850e-47
Msa0191450	Msa0619230	0.876653	1.177175e-68	-8.615850e-47
Msa0191450	Msa0622230	0.830006	3.744027e-55	-8.615850e-47
Msa0191450	Msa0643960	0.832211	1.075664e-55	-8.615850e-47
Msa0191450	Msa0649890	0.864066	1.588907e-64	-8.615850e-47
Msa0191450	Msa0653060	0.872387	3.307818e-67	-8.615850e-47
Msa0191450	Msa0681220	0.815285	9.999505e-52	-8.615850e-47
Msa0191450	Msa0686550	0.867935	9.483796e-66	-8.615850e-47
Msa0191450	Msa0694400	0.860971	1.423371e-63	-8.615850e-47
Msa0191450	Msa0704860	0.850824	1.322911e-60	-8.615850e-47
Msa0191450	Msa0705800	0.903110	4.865417e-79	-8.615850e-47
Msa0191450	Msa0708630	0.846977	1.546976e-59	-8.615850e-47
Msa0191450	Msa0712610	0.844696	6.441370e-59	-8.615850e-47
Msa0191450	Msa0717380	0.802851	4.610932e-49	-8.615850e-47
Msa0191450	Msa0748350	0.832048	1.180760e-55	-8.615850e-47
Msa0191450	Msa0752910	0.818648	1.756330e-52	-8.615850e-47
Msa0191450	Msa0758420	0.844312	8.171999e-59	-8.615850e-47
Msa0191450	Msa0776890	0.815799	7.680607e-52	-8.615850e-47
Msa0191450	Msa0788380	0.904641	9.920251e-80	-8.615850e-47
Msa0191450	Msa0799830	0.831512	1.600341e-55	-8.615850e-47
Msa0191450	Msa0821070	0.824561	7.549304e-54	-8.615850e-47
Msa0191450	Msa0822580	0.895721	7.323655e-76	-8.615850e-47
Msa0191450	Msa0834220	0.805827	1.106101e-49	-8.615850e-47
Msa0191450	Msa0838070	0.855312	6.863400e-62	-8.615850e-47
Msa0191450	Msa0858010	0.806522	7.896137e-50	-8.615850e-47
Msa0191450	Msa0861320	0.844554	7.035602e-59	-8.615850e-47
Msa0191450	Msa0915540	0.854018	1.627778e-61	-8.615850e-47
Msa0191450	Msa0929680	0.808138	3.588891e-50	-8.615850e-47
Msa0191450	Msa0929700	0.876528	1.300004e-68	-8.615850e-47
Msa0191450	Msa0929790	0.819757	9.823486e-53	-8.615850e-47
Msa0191450	Msa0930100	0.801064	1.074060e-48	-8.615850e-47
Msa0191450	Msa0930790	0.828194	1.029637e-54	-8.615850e-47
Msa0191450	Msa1021120	0.804923	1.711241e-49	-8.615850e-47
Msa0191450	Msa1037290	0.911708	4.476160e-83	-8.615850e-47
Msa0191450	Msa1040160	0.877840	4.548916e-69	-8.615850e-47
Msa0191450	Msa1041680	0.899361	2.139596e-77	-8.615850e-47
Msa0191450	Msa1042100	0.846055	2.761164e-59	-8.615850e-47
Msa0191450	Msa1054280	0.833970	3.927824e-56	-8.615850e-47
Msa0191450	Msa1058700	0.808686	2.741605e-50	-8.615850e-47
Msa0191450	Msa1078870	0.813094	3.045704e-51	-8.615850e-47
Msa0191450	Msa1078980	0.816147	6.424005e-52	-8.615850e-47
Msa0191450	Msa1080350	0.830284	3.201877e-55	-8.615850e-47
Msa0191450	Msa1082170	0.893621	5.298996e-75	-8.615850e-47
Msa0191450	Msa1083370	0.875441	3.074114e-68	-8.615850e-47
Msa0191450	Msa1096900	0.834200	3.438885e-56	-8.615850e-47
Msa0191450	Msa1116940	0.810919	9.073722e-51	-8.615850e-47
Msa0191450	Msa1124850	0.801348	9.392700e-49	-8.615850e-47
Msa0191450	Msa1125860	0.817758	2.793138e-52	-8.615850e-47
Msa0191450	Msa1126060	0.819020	1.446130e-52	-8.615850e-47
Msa0191450	Msa1126520	0.898076	7.559568e-77	-8.615850e-47
Msa0191450	Msa1126690	0.817832	2.687325e-52	-8.615850e-47
Msa0191450	Msa1156830	0.814152	1.781899e-51	-8.615850e-47
Msa0191450	Msa1164150	0.927302	1.420920e-91	-8.615850e-47
Msa0191450	Msa1165640	0.833905	4.075924e-56	-8.615850e-47
Msa0191450	Msa1166570	0.903251	4.207223e-79	-8.615850e-47
Msa0191450	Msa1167540	0.811361	7.275489e-51	-8.615850e-47
Msa0191450	Msa1169180	0.812772	3.583797e-51	-8.615850e-47
Msa0191450	Msa1180000	0.855579	5.737709e-62	-8.615850e-47
Msa0191450	Msa1186030	0.896431	3.713490e-76	-8.615850e-47
Msa0191450	Msa1206240	0.813138	2.978633e-51	-8.615850e-47
Msa0191450	Msa1218170	0.869505	2.944339e-66	-8.615850e-47
Msa0191450	Msa1235950	0.826948	2.049581e-54	-8.615850e-47
Msa0191450	Msa1235980	0.838156	3.399508e-57	-8.615850e-47
Msa0191450	Msa1237040	0.846296	2.375192e-59	-8.615850e-47
Msa0191450	Msa1240270	0.898745	3.926872e-77	-8.615850e-47
Msa0191450	Msa1258540	0.884313	2.134231e-71	-8.615850e-47
Msa0191450	Msa1270640	0.848604	5.511998e-60	-8.615850e-47
Msa0191450	Msa1270710	0.842390	2.662240e-58	-8.615850e-47
Msa0191450	Msa1278130	0.818666	1.740224e-52	-8.615850e-47
Msa0191450	Msa1284060	0.807869	4.094865e-50	-8.615850e-47
Msa0191450	Msa1295150	0.817772	2.773102e-52	-8.615850e-47
Msa0191450	Msa1301640	0.866049	3.786061e-65	-8.615850e-47
Msa0191450	Msa1311400	0.880159	6.905527e-70	-8.615850e-47
Msa0191450	Msa1317390	0.861365	1.079686e-63	-8.615850e-47
Msa0191450	Msa1332250	0.814749	1.314793e-51	-8.615850e-47
Msa0191450	Msa1351020	0.889648	2.007310e-73	-8.615850e-47
Msa0191450	Msa1351910	0.809717	1.647894e-50	-8.615850e-47
Msa0191450	Msa1352140	0.834006	3.845995e-56	-8.615850e-47
Msa0191450	Msa1356790	0.841055	5.991081e-58	-8.615850e-47
Msa0191450	Msa1358710	0.803185	3.933582e-49	-8.615850e-47
Msa0191450	Msa1363910	0.836014	1.199488e-56	-8.615850e-47
Msa0191450	Msa1368920	0.912904	1.141085e-83	-8.615850e-47
Msa0191450	Msa1374240	0.827206	1.777826e-54	-8.615850e-47
Msa0191450	Msa1374480	0.813357	2.666258e-51	-8.615850e-47
Msa0191450	Msa1385480	0.833276	5.853994e-56	-8.615850e-47
Msa0191450	Msa1390760	0.873233	1.723027e-67	-8.615850e-47
Msa0191450	Msa1392590	0.835312	1.805465e-56	-8.615850e-47
Msa0191450	Msa1397100	0.867494	1.313383e-65	-8.615850e-47
Msa0191450	Msa1402410	0.833992	3.876834e-56	-8.615850e-47
Msa0191450	Msa1403910	0.878486	2.700577e-69	-8.615850e-47
Msa0191450	Msa1407550	0.822661	2.102364e-53	-8.615850e-47
Msa0191450	Msa1418770	0.851950	6.356299e-61	-8.615850e-47
Msa0191450	Msa1426120	0.813555	2.412314e-51	-8.615850e-47
Msa0191450	Msa1432310	0.856762	2.583727e-62	-8.615850e-47
Msa0191450	Msa1446410	0.880813	4.030176e-70	-8.615850e-47
Msa0191450	Msa1449160	0.852383	4.786428e-61	-8.615850e-47
Msa0191450	Msa1450560	0.807144	5.833250e-50	-8.615850e-47
Msa0191450	Msa1453530	0.807131	5.872784e-50	-8.615850e-47
Msa0191450	Msa1463570	0.894698	1.930396e-75	-8.615850e-47
Msa0191450	Msa1463580	0.807885	4.062167e-50	-8.615850e-47
Msa0191450	Msa1465700	0.843477	1.367809e-58	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0191450	MtrunA17_Chr2g0281331	100.000	146	0	0	1	146	1	146	8.25e-104	293
Msa0191450	MtrunA17_Chr5g0401191	95.205	146	7	0	1	146	1	146	1.61e-90	259
Msa0191450	MtrunA17_Chr5g0447011	91.096	146	13	0	1	146	1	146	3.37e-87	251
Msa0191450	MtrunA17_Chr8g0370201	56.977	86	10	1	30	115	1	59	2.09e-23	87.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0191450	AT5G67510.1	88.356	146	17	0	1	146	1	146	1.04e-92	265
Msa0191450	AT3G49910.1	85.616	146	21	0	1	146	1	146	1.23e-91	262

Find 50 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTATGCAGACCGTTGATT+AGG	0.363868	2_1:-7080342	None:intergenic
CATTGAACGGACGTTGTACT+TGG	0.377101	2_1:+7080643	None:intergenic
TCGCAAAGACGACGAAGTTC+AGG	0.379749	2_1:-7080615	Msa0191450:CDS
TTAGTGATAACAACCTTTGA+AGG	0.395167	2_1:+7080464	None:intergenic
ACTTCGTCGTCTTTGCGAAC+AGG	0.416424	2_1:+7080620	None:intergenic
ATGGATCTACCGTTAATGTT+GGG	0.416486	2_1:-7080493	Msa0191450:CDS
GGGTCGTGCTGCTGCTGATA+AGG	0.420559	2_1:-7080393	Msa0191450:CDS
ACGGCTCTTCCTACGGCTGC+TGG	0.422834	2_1:+7080739	None:intergenic
AAGACACGGCGGACGCTTGA+TGG	0.437229	2_1:+7080698	None:intergenic
CACTAAGCTCAGATTGGATA+AGG	0.438891	2_1:-7080447	Msa0191450:CDS
CCTGAGTAACCTTACCTTCA+CGG	0.446679	2_1:+7080570	None:intergenic
AGGATTAACCCCAACATTAA+CGG	0.448396	2_1:+7080484	None:intergenic
TGTTATCACTAAGCTCAGAT+TGG	0.466490	2_1:-7080453	Msa0191450:CDS
GACGAAGTTCAGGTCGTTCG+TGG	0.488796	2_1:-7080605	Msa0191450:CDS
ATTCAAAGGCCGTGAAGGTA+AGG	0.498862	2_1:-7080579	Msa0191450:CDS
CTCAGGTTTACCGTCGCAAG+TGG	0.512762	2_1:-7080553	Msa0191450:CDS
TGTTGGGGTTAATCCTTCAA+AGG	0.524932	2_1:-7080477	Msa0191450:CDS
GCTACTTGATCGTAAGGCTA+AGG	0.528270	2_1:-7080414	Msa0191450:CDS
AATGGATCTACCGTTAATGT+TGG	0.529601	2_1:-7080494	Msa0191450:CDS
CTACGGCTGCTGGAAACTCG+TGG	0.530535	2_1:+7080749	None:intergenic
TGCTGCTGCTGATAAGGAGA+AGG	0.537077	2_1:-7080387	Msa0191450:CDS
GAGCCGTAAGGCTCATTTCA+CGG	0.545137	2_1:-7080723	Msa0191450:CDS
ATCACAAGGGAGAAGGTGAA+TGG	0.545267	2_1:-7080512	Msa0191450:CDS
GAGCCTTACGGCTCTTCCTA+CGG	0.547173	2_1:+7080732	None:intergenic
CTACTTGATCGTAAGGCTAA+GGG	0.553374	2_1:-7080413	Msa0191450:CDS
GTCGTTCGTGGAACATTCAA+AGG	0.554383	2_1:-7080593	Msa0191450:CDS
TAAATCGCTACTTGATCGTA+AGG	0.555125	2_1:-7080420	Msa0191450:CDS
GGGTACTAAGTTTGCTCCTG+AGG	0.563912	2_1:-7080366	Msa0191450:CDS
TGGATCTACCGTTAATGTTG+GGG	0.564613	2_1:-7080492	Msa0191450:CDS
AACGGACGTTGTACTTGGAT+CGG	0.564652	2_1:+7080648	None:intergenic
CCGTGAAGGTAAGGTTACTC+AGG	0.566699	2_1:-7080570	Msa0191450:CDS
CTTTGCGAACAGGCATTGAA+CGG	0.570326	2_1:+7080630	None:intergenic
GTTGTACTTGGATCGGAGAT+CGG	0.572814	2_1:+7080655	None:intergenic
ACGGTCTGCATAATATCCTC+AGG	0.575139	2_1:+7080350	None:intergenic
GCGCCGTGAAATGAGCCTTA+CGG	0.575259	2_1:+7080720	None:intergenic
CGAACGCATCACAAGGGAGA+AGG	0.580140	2_1:-7080519	Msa0191450:CDS
GATCGGAGATCGGCGGAGAG+TGG	0.584970	2_1:+7080665	None:intergenic
TACGGCTGCTGGAAACTCGT+GGG	0.586325	2_1:+7080750	None:intergenic
TTCACATCGAACGCATCACA+AGG	0.588472	2_1:-7080526	Msa0191450:CDS
CAGCCGTAGGAAGAGCCGTA+AGG	0.591177	2_1:-7080735	Msa0191450:CDS
GGAACATTCAAAGGCCGTGA+AGG	0.606062	2_1:-7080584	Msa0191450:CDS
GATTCAAGAATCCTAATCAA+CGG	0.625900	2_1:+7080331	None:intergenic
GCTGCTGCTGATAAGGAGAA+GGG	0.634378	2_1:-7080386	Msa0191450:CDS
TGTGAATCACCCACTTGCGA+CGG	0.658219	2_1:+7080543	None:intergenic
TCAGGTTTACCGTCGCAAGT+GGG	0.670860	2_1:-7080552	Msa0191450:CDS
CACGAGTTTCCAGCAGCCGT+AGG	0.686122	2_1:-7080748	Msa0191450:CDS
GTGGCGCGCTCATTAAGACA+CGG	0.705562	2_1:+7080684	None:intergenic
GTACTTGGATCGGAGATCGG+CGG	0.709980	2_1:+7080658	None:intergenic
TCACATCGAACGCATCACAA+GGG	0.710980	2_1:-7080525	Msa0191450:CDS
GCGCGCTCATTAAGACACGG+CGG	0.823768	2_1:+7080687	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAGTGATAACAACCTTTGA+AGG	+	chr2_1:7080643-7080662	None:intergenic	30.0%
	AATGGATCTACCGTTAATGT+TGG	-	chr2_1:7080610-7080629	Msa0191450:CDS	35.0%
	ATGGATCTACCGTTAATGTT+GGG	-	chr2_1:7080611-7080630	Msa0191450:CDS	35.0%
	AGGATTAACCCCAACATTAA+CGG	+	chr2_1:7080623-7080642	None:intergenic	35.0%
	TGTTATCACTAAGCTCAGAT+TGG	-	chr2_1:7080651-7080670	Msa0191450:CDS	35.0%
	TAAATCGCTACTTGATCGTA+AGG	-	chr2_1:7080684-7080703	Msa0191450:CDS	35.0%
	TGGATCTACCGTTAATGTTG+GGG	-	chr2_1:7080612-7080631	Msa0191450:CDS	40.0%
	TGTTGGGGTTAATCCTTCAA+AGG	-	chr2_1:7080627-7080646	Msa0191450:CDS	40.0%
	CACTAAGCTCAGATTGGATA+AGG	-	chr2_1:7080657-7080676	Msa0191450:CDS	40.0%
	CTACTTGATCGTAAGGCTAA+GGG	-	chr2_1:7080691-7080710	Msa0191450:CDS	40.0%
	GTTGTACTTGGATCGGAGAT+CGG	+	chr2_1:7080452-7080471	None:intergenic	45.0%
	AACGGACGTTGTACTTGGAT+CGG	+	chr2_1:7080459-7080478	None:intergenic	45.0%
	CATTGAACGGACGTTGTACT+TGG	+	chr2_1:7080464-7080483	None:intergenic	45.0%
!!	CTTTGCGAACAGGCATTGAA+CGG	+	chr2_1:7080477-7080496	None:intergenic	45.0%
	GTCGTTCGTGGAACATTCAA+AGG	-	chr2_1:7080511-7080530	Msa0191450:CDS	45.0%
	ATTCAAAGGCCGTGAAGGTA+AGG	-	chr2_1:7080525-7080544	Msa0191450:CDS	45.0%
	CCTGAGTAACCTTACCTTCA+CGG	+	chr2_1:7080537-7080556	None:intergenic	45.0%
	TTCACATCGAACGCATCACA+AGG	-	chr2_1:7080578-7080597	Msa0191450:CDS	45.0%
	TCACATCGAACGCATCACAA+GGG	-	chr2_1:7080579-7080598	Msa0191450:CDS	45.0%
	ATCACAAGGGAGAAGGTGAA+TGG	-	chr2_1:7080592-7080611	Msa0191450:CDS	45.0%
	GCTACTTGATCGTAAGGCTA+AGG	-	chr2_1:7080690-7080709	Msa0191450:CDS	45.0%
	ACGGTCTGCATAATATCCTC+AGG	+	chr2_1:7080757-7080776	None:intergenic	45.0%
	GAGCCGTAAGGCTCATTTCA+CGG	-	chr2_1:7080381-7080400	Msa0191450:CDS	50.0%
	ACTTCGTCGTCTTTGCGAAC+AGG	+	chr2_1:7080487-7080506	None:intergenic	50.0%
	TCGCAAAGACGACGAAGTTC+AGG	-	chr2_1:7080489-7080508	Msa0191450:CDS	50.0%
	GGAACATTCAAAGGCCGTGA+AGG	-	chr2_1:7080520-7080539	Msa0191450:CDS	50.0%
	CCGTGAAGGTAAGGTTACTC+AGG	-	chr2_1:7080534-7080553	Msa0191450:CDS	50.0%
	TCAGGTTTACCGTCGCAAGT+GGG	-	chr2_1:7080552-7080571	Msa0191450:CDS	50.0%
	TGTGAATCACCCACTTGCGA+CGG	+	chr2_1:7080564-7080583	None:intergenic	50.0%
	TGCTGCTGCTGATAAGGAGA+AGG	-	chr2_1:7080717-7080736	Msa0191450:CDS	50.0%
	GCTGCTGCTGATAAGGAGAA+GGG	-	chr2_1:7080718-7080737	Msa0191450:CDS	50.0%
!	GGGTACTAAGTTTGCTCCTG+AGG	-	chr2_1:7080738-7080757	Msa0191450:CDS	50.0%
	TACGGCTGCTGGAAACTCGT+GGG	+	chr2_1:7080357-7080376	None:intergenic	55.0%
	GAGCCTTACGGCTCTTCCTA+CGG	+	chr2_1:7080375-7080394	None:intergenic	55.0%
	GCGCCGTGAAATGAGCCTTA+CGG	+	chr2_1:7080387-7080406	None:intergenic	55.0%
	GTGGCGCGCTCATTAAGACA+CGG	+	chr2_1:7080423-7080442	None:intergenic	55.0%
	GTACTTGGATCGGAGATCGG+CGG	+	chr2_1:7080449-7080468	None:intergenic	55.0%
	GACGAAGTTCAGGTCGTTCG+TGG	-	chr2_1:7080499-7080518	Msa0191450:CDS	55.0%
	CTCAGGTTTACCGTCGCAAG+TGG	-	chr2_1:7080551-7080570	Msa0191450:CDS	55.0%
	CGAACGCATCACAAGGGAGA+AGG	-	chr2_1:7080585-7080604	Msa0191450:CDS	55.0%
	CTACGGCTGCTGGAAACTCG+TGG	+	chr2_1:7080358-7080377	None:intergenic	60.0%
	CACGAGTTTCCAGCAGCCGT+AGG	-	chr2_1:7080356-7080375	Msa0191450:CDS	60.0%
	CAGCCGTAGGAAGAGCCGTA+AGG	-	chr2_1:7080369-7080388	Msa0191450:CDS	60.0%
	AAGACACGGCGGACGCTTGA+TGG	+	chr2_1:7080409-7080428	None:intergenic	60.0%
	GCGCGCTCATTAAGACACGG+CGG	+	chr2_1:7080420-7080439	None:intergenic	60.0%
!	GGGTCGTGCTGCTGCTGATA+AGG	-	chr2_1:7080711-7080730	Msa0191450:CDS	60.0%
	ACGGCTCTTCCTACGGCTGC+TGG	+	chr2_1:7080368-7080387	None:intergenic	65.0%
	GATCGGAGATCGGCGGAGAG+TGG	+	chr2_1:7080442-7080461	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	7080343	7080783	7080343	ID=Msa0191450;Name=Msa0191450
chr2_1	mRNA	7080343	7080783	7080343	ID=Msa0191450-mRNA-1;Parent=Msa0191450;Name=Msa0191450-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|146
chr2_1	exon	7080343	7080783	7080343	ID=Msa0191450-mRNA-1:exon:3206;Parent=Msa0191450-mRNA-1
chr2_1	CDS	7080343	7080783	7080343	ID=Msa0191450-mRNA-1:cds;Parent=Msa0191450-mRNA-1

Gene Sequence

>Msa0191450

ATGAAGTTCAACCCACGAGTTTCCAGCAGCCGTAGGAAGAGCCGTAAGGCTCATTTCACGGCGCCATCAAGCGTCCGCCGTGTCTTAATGAGCGCGCCACTCTCCGCCGATCTCCGATCCAAGTACAACGTCCGTTCAATGCCTGTTCGCAAAGACGACGAAGTTCAGGTCGTTCGTGGAACATTCAAAGGCCGTGAAGGTAAGGTTACTCAGGTTTACCGTCGCAAGTGGGTGATTCACATCGAACGCATCACAAGGGAGAAGGTGAATGGATCTACCGTTAATGTTGGGGTTAATCCTTCAAAGGTTGTTATCACTAAGCTCAGATTGGATAAGGATCGTAAATCGCTACTTGATCGTAAGGCTAAGGGTCGTGCTGCTGCTGATAAGGAGAAGGGTACTAAGTTTGCTCCTGAGGATATTATGCAGACCGTTGATTAG

Protein sequence

>Msa0191450

MKFNPRVSSSRRKSRKAHFTAPSSVRRVLMSAPLSADLRSKYNVRSMPVRKDDEVQVVRGTFKGREGKVTQVYRRKWVIHIERITREKVNGSTVNVGVNPSKVVITKLRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQTVD*