AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0233550


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0233550 MtrunA17_Chr2g0281331 100.000 146 0 0 1 146 1 146 8.25e-104 293
Msa0233550 MtrunA17_Chr5g0401191 95.205 146 7 0 1 146 1 146 1.61e-90 259
Msa0233550 MtrunA17_Chr5g0447011 91.096 146 13 0 1 146 1 146 3.37e-87 251
Msa0233550 MtrunA17_Chr8g0370201 56.977 86 10 1 30 115 1 59 2.09e-23 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0233550 AT5G67510.1 88.356 146 17 0 1 146 1 146 1.04e-92 265
Msa0233550 AT3G49910.1 85.616 146 21 0 1 146 1 146 1.23e-91 262

Find 50 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATGCAGACCGTTGATT+AGG 0.363868 2_2:-5900600 None:intergenic
CATTGAACGGACGTTGTACT+TGG 0.377101 2_2:+5900901 None:intergenic
TCGCAAAGACGACGAAGTTC+AGG 0.379749 2_2:-5900873 Msa0233550:CDS
TTAGTGATAACAACCTTTGA+AGG 0.395167 2_2:+5900722 None:intergenic
ACTTCGTCGTCTTTGCGAAC+AGG 0.416424 2_2:+5900878 None:intergenic
ATGGATCTACCGTTAATGTT+GGG 0.416486 2_2:-5900751 Msa0233550:CDS
GGGTCGTGCTGCTGCTGATA+AGG 0.420559 2_2:-5900651 Msa0233550:CDS
ACGGCTCTTCCTACGGCTGC+TGG 0.422834 2_2:+5900997 None:intergenic
AAGACACGGCGGACGCTTGA+TGG 0.437229 2_2:+5900956 None:intergenic
CACTAAGCTCAGATTGGATA+AGG 0.438891 2_2:-5900705 Msa0233550:CDS
CCTGAGTAACCTTACCTTCA+CGG 0.446679 2_2:+5900828 None:intergenic
AGGATTAACCCCAACATTAA+CGG 0.448396 2_2:+5900742 None:intergenic
TGTTATCACTAAGCTCAGAT+TGG 0.466490 2_2:-5900711 Msa0233550:CDS
GACGAAGTTCAGGTCGTTCG+TGG 0.488796 2_2:-5900863 Msa0233550:CDS
ATTCAAAGGCCGTGAAGGTA+AGG 0.498862 2_2:-5900837 Msa0233550:CDS
CTCAGGTTTACCGTCGCAAG+TGG 0.512762 2_2:-5900811 Msa0233550:CDS
TGTTGGGGTTAATCCTTCAA+AGG 0.524932 2_2:-5900735 Msa0233550:CDS
GCTACTTGATCGTAAGGCTA+AGG 0.528270 2_2:-5900672 Msa0233550:CDS
AATGGATCTACCGTTAATGT+TGG 0.529601 2_2:-5900752 Msa0233550:CDS
CTACGGCTGCTGGAAACTCG+TGG 0.530535 2_2:+5901007 None:intergenic
TGCTGCTGCTGATAAGGAGA+AGG 0.537077 2_2:-5900645 Msa0233550:CDS
GAGCCGTAAGGCTCATTTCA+CGG 0.545137 2_2:-5900981 Msa0233550:CDS
ATCACAAGGGAGAAGGTGAA+TGG 0.545267 2_2:-5900770 Msa0233550:CDS
GAGCCTTACGGCTCTTCCTA+CGG 0.547173 2_2:+5900990 None:intergenic
CTACTTGATCGTAAGGCTAA+GGG 0.553374 2_2:-5900671 Msa0233550:CDS
GTCGTTCGTGGAACATTCAA+AGG 0.554383 2_2:-5900851 Msa0233550:CDS
TAAATCGCTACTTGATCGTA+AGG 0.555125 2_2:-5900678 Msa0233550:CDS
GGGTACTAAGTTTGCTCCTG+AGG 0.563912 2_2:-5900624 Msa0233550:CDS
TGGATCTACCGTTAATGTTG+GGG 0.564613 2_2:-5900750 Msa0233550:CDS
AACGGACGTTGTACTTGGAT+CGG 0.564652 2_2:+5900906 None:intergenic
CCGTGAAGGTAAGGTTACTC+AGG 0.566699 2_2:-5900828 Msa0233550:CDS
CTTTGCGAACAGGCATTGAA+CGG 0.570326 2_2:+5900888 None:intergenic
GTTGTACTTGGATCGGAGAT+CGG 0.572814 2_2:+5900913 None:intergenic
ACGGTCTGCATAATATCCTC+AGG 0.575139 2_2:+5900608 None:intergenic
GCGCCGTGAAATGAGCCTTA+CGG 0.575259 2_2:+5900978 None:intergenic
CGAACGCATCACAAGGGAGA+AGG 0.580140 2_2:-5900777 Msa0233550:CDS
GATCGGAGATCGGCGGAGAG+TGG 0.584970 2_2:+5900923 None:intergenic
TACGGCTGCTGGAAACTCGT+GGG 0.586325 2_2:+5901008 None:intergenic
TTCACATCGAACGCATCACA+AGG 0.588472 2_2:-5900784 Msa0233550:CDS
CAGCCGTAGGAAGAGCCGTA+AGG 0.591177 2_2:-5900993 Msa0233550:CDS
GGAACATTCAAAGGCCGTGA+AGG 0.606062 2_2:-5900842 Msa0233550:CDS
GATTCAAGAATCCTAATCAA+CGG 0.625900 2_2:+5900589 None:intergenic
GCTGCTGCTGATAAGGAGAA+GGG 0.634378 2_2:-5900644 Msa0233550:CDS
TGTGAATCACCCACTTGCGA+CGG 0.658219 2_2:+5900801 None:intergenic
TCAGGTTTACCGTCGCAAGT+GGG 0.670860 2_2:-5900810 Msa0233550:CDS
CACGAGTTTCCAGCAGCCGT+AGG 0.686122 2_2:-5901006 Msa0233550:CDS
GTGGCGCGCTCATTAAGACA+CGG 0.705562 2_2:+5900942 None:intergenic
GTACTTGGATCGGAGATCGG+CGG 0.709980 2_2:+5900916 None:intergenic
TCACATCGAACGCATCACAA+GGG 0.710980 2_2:-5900783 Msa0233550:CDS
GCGCGCTCATTAAGACACGG+CGG 0.823768 2_2:+5900945 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTAGTGATAACAACCTTTGA+AGG + chr2_2:5900901-5900920 None:intergenic 30.0%
AATGGATCTACCGTTAATGT+TGG - chr2_2:5900868-5900887 Msa0233550:CDS 35.0%
ATGGATCTACCGTTAATGTT+GGG - chr2_2:5900869-5900888 Msa0233550:CDS 35.0%
AGGATTAACCCCAACATTAA+CGG + chr2_2:5900881-5900900 None:intergenic 35.0%
TGTTATCACTAAGCTCAGAT+TGG - chr2_2:5900909-5900928 Msa0233550:CDS 35.0%
TAAATCGCTACTTGATCGTA+AGG - chr2_2:5900942-5900961 Msa0233550:CDS 35.0%
TGGATCTACCGTTAATGTTG+GGG - chr2_2:5900870-5900889 Msa0233550:CDS 40.0%
TGTTGGGGTTAATCCTTCAA+AGG - chr2_2:5900885-5900904 Msa0233550:CDS 40.0%
CACTAAGCTCAGATTGGATA+AGG - chr2_2:5900915-5900934 Msa0233550:CDS 40.0%
CTACTTGATCGTAAGGCTAA+GGG - chr2_2:5900949-5900968 Msa0233550:CDS 40.0%
GTTGTACTTGGATCGGAGAT+CGG + chr2_2:5900710-5900729 None:intergenic 45.0%
AACGGACGTTGTACTTGGAT+CGG + chr2_2:5900717-5900736 None:intergenic 45.0%
CATTGAACGGACGTTGTACT+TGG + chr2_2:5900722-5900741 None:intergenic 45.0%
!! CTTTGCGAACAGGCATTGAA+CGG + chr2_2:5900735-5900754 None:intergenic 45.0%
GTCGTTCGTGGAACATTCAA+AGG - chr2_2:5900769-5900788 Msa0233550:CDS 45.0%
ATTCAAAGGCCGTGAAGGTA+AGG - chr2_2:5900783-5900802 Msa0233550:CDS 45.0%
CCTGAGTAACCTTACCTTCA+CGG + chr2_2:5900795-5900814 None:intergenic 45.0%
TTCACATCGAACGCATCACA+AGG - chr2_2:5900836-5900855 Msa0233550:CDS 45.0%
TCACATCGAACGCATCACAA+GGG - chr2_2:5900837-5900856 Msa0233550:CDS 45.0%
ATCACAAGGGAGAAGGTGAA+TGG - chr2_2:5900850-5900869 Msa0233550:CDS 45.0%
GCTACTTGATCGTAAGGCTA+AGG - chr2_2:5900948-5900967 Msa0233550:CDS 45.0%
ACGGTCTGCATAATATCCTC+AGG + chr2_2:5901015-5901034 None:intergenic 45.0%
GAGCCGTAAGGCTCATTTCA+CGG - chr2_2:5900639-5900658 Msa0233550:CDS 50.0%
ACTTCGTCGTCTTTGCGAAC+AGG + chr2_2:5900745-5900764 None:intergenic 50.0%
TCGCAAAGACGACGAAGTTC+AGG - chr2_2:5900747-5900766 Msa0233550:CDS 50.0%
GGAACATTCAAAGGCCGTGA+AGG - chr2_2:5900778-5900797 Msa0233550:CDS 50.0%
CCGTGAAGGTAAGGTTACTC+AGG - chr2_2:5900792-5900811 Msa0233550:CDS 50.0%
TCAGGTTTACCGTCGCAAGT+GGG - chr2_2:5900810-5900829 Msa0233550:CDS 50.0%
TGTGAATCACCCACTTGCGA+CGG + chr2_2:5900822-5900841 None:intergenic 50.0%
TGCTGCTGCTGATAAGGAGA+AGG - chr2_2:5900975-5900994 Msa0233550:CDS 50.0%
GCTGCTGCTGATAAGGAGAA+GGG - chr2_2:5900976-5900995 Msa0233550:CDS 50.0%
! GGGTACTAAGTTTGCTCCTG+AGG - chr2_2:5900996-5901015 Msa0233550:CDS 50.0%
TACGGCTGCTGGAAACTCGT+GGG + chr2_2:5900615-5900634 None:intergenic 55.0%
GAGCCTTACGGCTCTTCCTA+CGG + chr2_2:5900633-5900652 None:intergenic 55.0%
GCGCCGTGAAATGAGCCTTA+CGG + chr2_2:5900645-5900664 None:intergenic 55.0%
GTGGCGCGCTCATTAAGACA+CGG + chr2_2:5900681-5900700 None:intergenic 55.0%
GTACTTGGATCGGAGATCGG+CGG + chr2_2:5900707-5900726 None:intergenic 55.0%
GACGAAGTTCAGGTCGTTCG+TGG - chr2_2:5900757-5900776 Msa0233550:CDS 55.0%
CTCAGGTTTACCGTCGCAAG+TGG - chr2_2:5900809-5900828 Msa0233550:CDS 55.0%
CGAACGCATCACAAGGGAGA+AGG - chr2_2:5900843-5900862 Msa0233550:CDS 55.0%
CTACGGCTGCTGGAAACTCG+TGG + chr2_2:5900616-5900635 None:intergenic 60.0%
CACGAGTTTCCAGCAGCCGT+AGG - chr2_2:5900614-5900633 Msa0233550:CDS 60.0%
CAGCCGTAGGAAGAGCCGTA+AGG - chr2_2:5900627-5900646 Msa0233550:CDS 60.0%
AAGACACGGCGGACGCTTGA+TGG + chr2_2:5900667-5900686 None:intergenic 60.0%
GCGCGCTCATTAAGACACGG+CGG + chr2_2:5900678-5900697 None:intergenic 60.0%
! GGGTCGTGCTGCTGCTGATA+AGG - chr2_2:5900969-5900988 Msa0233550:CDS 60.0%
ACGGCTCTTCCTACGGCTGC+TGG + chr2_2:5900626-5900645 None:intergenic 65.0%
GATCGGAGATCGGCGGAGAG+TGG + chr2_2:5900700-5900719 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 5900601 5901041 5900601 ID=Msa0233550;Name=Msa0233550
chr2_2 mRNA 5900601 5901041 5900601 ID=Msa0233550-mRNA-1;Parent=Msa0233550;Name=Msa0233550-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|146
chr2_2 exon 5900601 5901041 5900601 ID=Msa0233550-mRNA-1:exon:2593;Parent=Msa0233550-mRNA-1
chr2_2 CDS 5900601 5901041 5900601 ID=Msa0233550-mRNA-1:cds;Parent=Msa0233550-mRNA-1
Gene Sequence

>Msa0233550

ATGAAGTTCAACCCACGAGTTTCCAGCAGCCGTAGGAAGAGCCGTAAGGCTCATTTCACGGCGCCATCAAGCGTCCGCCGTGTCTTAATGAGCGCGCCACTCTCCGCCGATCTCCGATCCAAGTACAACGTCCGTTCAATGCCTGTTCGCAAAGACGACGAAGTTCAGGTCGTTCGTGGAACATTCAAAGGCCGTGAAGGTAAGGTTACTCAGGTTTACCGTCGCAAGTGGGTGATTCACATCGAACGCATCACAAGGGAGAAGGTGAATGGATCTACCGTTAATGTTGGGGTTAATCCTTCAAAGGTTGTTATCACTAAGCTCAGATTGGATAAGGATCGTAAATCGCTACTTGATCGTAAGGCTAAGGGTCGTGCTGCTGCTGATAAGGAGAAGGGTACTAAGTTTGCTCCTGAGGATATTATGCAGACCGTTGATTAG

Protein sequence

>Msa0233550

MKFNPRVSSSRRKSRKAHFTAPSSVRRVLMSAPLSADLRSKYNVRSMPVRKDDEVQVVRGTFKGREGKVTQVYRRKWVIHIERITREKVNGSTVNVGVNPSKVVITKLRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQTVD*