AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1406150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1406150 MtrunA17_Chr7g0217851 58.407 226 19 3 32 221 5 191 8.73e-70 211
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 60 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGCTGCTGCTGGCATCCA+AGG 0.252455 tig0023193:+48101 None:intergenic
GAAACGTGAATAACATTAAA+TGG 0.282735 tig0023193:+47719 None:intergenic
GTGTACTATGAAATGGCTTT+TGG 0.328699 tig0023193:-47932 Msa1406150:three_prime_UTR
CAGTTTCTTCTTCTTAGACT+TGG 0.357409 tig0023193:+49537 None:intergenic
AGTTATAAATATTGTTTCTC+TGG 0.361394 tig0023193:+47896 None:intergenic
TCTAAATTCACTCTGGTCAT+TGG 0.364019 tig0023193:-49086 Msa1406150:CDS
CGCCCAACTACTCTAGATAT+TGG 0.372061 tig0023193:-47998 Msa1406150:exon
ATCCAACAGCTGGCTGCTGC+TGG 0.372326 tig0023193:+48092 None:intergenic
AATAACATTAAATGGTAATC+TGG 0.373731 tig0023193:+47727 None:intergenic
TCCATTCCCGTTCGTTCCCA+TGG 0.377294 tig0023193:-49593 Msa1406150:exon
GTGCACATGCCAACACAATA+TGG 0.384828 tig0023193:-48526 Msa1406150:CDS
TGGCTGCTGCTGGCATCCAA+GGG 0.407095 tig0023193:+48102 None:intergenic
GTGAAGCTGTATCATCTTCA+TGG 0.410616 tig0023193:+48560 None:intergenic
CTTCTGAAATTCCTTCGATT+CGG 0.426782 tig0023193:+49652 None:intergenic
GGTCCAATATCTAGAGTAGT+TGG 0.437672 tig0023193:+47995 None:intergenic
TTATGATCAGATTTATCGTC+AGG 0.438391 tig0023193:-49401 Msa1406150:intron
CTGCATATCTGACCTTAGTT+AGG 0.441952 tig0023193:-47771 Msa1406150:three_prime_UTR
TAGTATGCATAGGAAGTAGA+AGG 0.447849 tig0023193:+47843 None:intergenic
AAGAGAAGAAGAGAGGAGAT+AGG 0.453487 tig0023193:+49713 None:intergenic
TTCTACTTCCTATGCATACT+AGG 0.457176 tig0023193:-47841 Msa1406150:three_prime_UTR
CCATTCCCGTTCGTTCCCAT+GGG 0.457296 tig0023193:-49592 Msa1406150:exon
ACCTCCTTGCCATATTGTGT+TGG 0.459988 tig0023193:+48517 None:intergenic
GGCATGTGCACAGGTTGAAT+TGG 0.460863 tig0023193:+48538 None:intergenic
CTCCACCCATGGGAACGAAC+GGG 0.475346 tig0023193:+49587 None:intergenic
CCGTTCGTTCCCATGGGTGG+AGG 0.484615 tig0023193:-49586 Msa1406150:exon
TGCCAGCAGCAGCCAGCTGT+TGG 0.490353 tig0023193:-48094 Msa1406150:CDS
TATTGTGTTGGCATGTGCAC+AGG 0.498215 tig0023193:+48529 None:intergenic
GCAGGCCCGCAAAGCAAAAG+AGG 0.498850 tig0023193:-49128 Msa1406150:CDS
GGAGATAGGCATAGGCTGAC+TGG 0.499223 tig0023193:+49727 None:intergenic
TTCCCGTTCGTTCCCATGGG+TGG 0.500541 tig0023193:-49589 Msa1406150:exon
CTGAAATTCCTTCGATTCGG+CGG 0.505307 tig0023193:+49655 None:intergenic
GTTGCAAGTGTACTATGAAA+TGG 0.509066 tig0023193:-47939 Msa1406150:three_prime_UTR
TGAAAATGATATGAAGCCCT+TGG 0.509252 tig0023193:-48118 Msa1406150:CDS
CTAGGGTTCCGCCGAATCGA+AGG 0.510517 tig0023193:-49663 Msa1406150:five_prime_UTR
GTCCAATATCTAGAGTAGTT+GGG 0.512418 tig0023193:+47996 None:intergenic
TGACCAATGTCGCCTAACTA+AGG 0.512656 tig0023193:+47759 None:intergenic
CCCATGGGAACGAACGGGAA+TGG 0.520437 tig0023193:+49592 None:intergenic
CCTCCACCCATGGGAACGAA+CGG 0.521612 tig0023193:+49586 None:intergenic
TGTTGAGCTCATCCAACAGC+TGG 0.537978 tig0023193:+48082 None:intergenic
TGACCTTAGTTAGGCGACAT+TGG 0.549340 tig0023193:-47762 Msa1406150:three_prime_UTR
AGCCTCTTCAGCTAATCGAC+GGG 0.554287 tig0023193:+49168 None:intergenic
CAACTTAGAAAAGAAAACCT+AGG 0.581444 tig0023193:-49681 Msa1406150:five_prime_UTR
ATAGGCTGACTGGTTGAGAG+AGG 0.582311 tig0023193:+49737 None:intergenic
ATAGTACACTTGCAACAACT+AGG 0.582344 tig0023193:+47946 None:intergenic
AATATTTATAACTAATAGTG+TGG 0.584031 tig0023193:-47886 Msa1406150:three_prime_UTR
GAATTGAAAGAGAAGAAGAG+AGG 0.585085 tig0023193:+49706 None:intergenic
CTCTTGCTCTAAATTCACTC+TGG 0.587930 tig0023193:-49093 Msa1406150:CDS
AGAAGAGAGGAGATAGGCAT+AGG 0.588072 tig0023193:+49719 None:intergenic
AGCCCGTCGATTAGCTGAAG+AGG 0.588284 tig0023193:-49170 Msa1406150:CDS
GAGCCTCTTCAGCTAATCGA+CGG 0.591168 tig0023193:+49167 None:intergenic
CAACTTCTCCTAGTATGCAT+AGG 0.595855 tig0023193:+47833 None:intergenic
AACTTAGAAAAGAAAACCTA+GGG 0.603719 tig0023193:-49680 Msa1406150:five_prime_UTR
CGACACAAAATAATAAACCT+AGG 0.609524 tig0023193:+47974 None:intergenic
CTACTCTAGATATTGGACCT+AGG 0.618103 tig0023193:-47991 Msa1406150:exon
GAAAAGCAGCTTTATCCTCA+AGG 0.621167 tig0023193:+48040 None:intergenic
AACACAAAACGTTAGCCTTG+AGG 0.622562 tig0023193:-48055 Msa1406150:CDS
TTGAATAAATAGCTTGACCA+AGG 0.629472 tig0023193:+47794 None:intergenic
GTGTTAGCATGTGCATACCT+TGG 0.659371 tig0023193:-47811 Msa1406150:three_prime_UTR
CATGCCAACACAATATGGCA+AGG 0.661413 tig0023193:-48521 Msa1406150:CDS
GCCAACACAATATGGCAAGG+AGG 0.737684 tig0023193:-48518 Msa1406150:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACTAAATATTATGAAAAT+TGG + tig0023193:47977-47996 None:intergenic 10.0%
!! ATACTAAATATTATGAAAAT+TGG + tig0023193:47977-47996 None:intergenic 10.0%
!! TTATAAGAAATTAAGCAAAA+GGG - tig0023193:49280-49299 Msa1406150:intron 15.0%
!!! ATTTTTAATTATGTATTTGC+AGG - tig0023193:49313-49332 Msa1406150:intron 15.0%
!! AATATTTATAACTAATAGTG+TGG - tig0023193:49575-49594 Msa1406150:CDS 15.0%
!! TTATAAGAAATTAAGCAAAA+GGG - tig0023193:49280-49299 Msa1406150:intron 15.0%
!!! ATTTTTAATTATGTATTTGC+AGG - tig0023193:49313-49332 Msa1406150:intron 15.0%
!! AATATTTATAACTAATAGTG+TGG - tig0023193:49575-49594 Msa1406150:CDS 15.0%
!!! TTTTAACTAACTTGTTATGT+TGG - tig0023193:48092-48111 Msa1406150:CDS 20.0%
!! ATGTTCTGTTATTGTTAATT+TGG - tig0023193:48118-48137 Msa1406150:CDS 20.0%
!!! TGTTATTGTTAATTTGGTTA+TGG - tig0023193:48124-48143 Msa1406150:CDS 20.0%
!!! ATTCATGAAATTTTGTAATC+TGG - tig0023193:48559-48578 Msa1406150:CDS 20.0%
!!! CATAGCTTTTCTTTAAAATT+GGG - tig0023193:49180-49199 Msa1406150:CDS 20.0%
!! AATTTCTTATAAGTAAGGAA+AGG + tig0023193:49272-49291 None:intergenic 20.0%
!! TGCTTAATTTCTTATAAGTA+AGG + tig0023193:49277-49296 None:intergenic 20.0%
!! CTTATAAGAAATTAAGCAAA+AGG - tig0023193:49279-49298 Msa1406150:intron 20.0%
!!! AGTTATAAATATTGTTTCTC+TGG + tig0023193:49568-49587 None:intergenic 20.0%
!! AATAACATTAAATGGTAATC+TGG + tig0023193:49737-49756 None:intergenic 20.0%
!!! TTTTAACTAACTTGTTATGT+TGG - tig0023193:48092-48111 Msa1406150:CDS 20.0%
!! ATGTTCTGTTATTGTTAATT+TGG - tig0023193:48118-48137 Msa1406150:CDS 20.0%
!!! TGTTATTGTTAATTTGGTTA+TGG - tig0023193:48124-48143 Msa1406150:CDS 20.0%
!!! ATTCATGAAATTTTGTAATC+TGG - tig0023193:48559-48578 Msa1406150:CDS 20.0%
!!! CATAGCTTTTCTTTAAAATT+GGG - tig0023193:49180-49199 Msa1406150:CDS 20.0%
!! AATTTCTTATAAGTAAGGAA+AGG + tig0023193:49272-49291 None:intergenic 20.0%
!! TGCTTAATTTCTTATAAGTA+AGG + tig0023193:49277-49296 None:intergenic 20.0%
!! CTTATAAGAAATTAAGCAAA+AGG - tig0023193:49279-49298 Msa1406150:intron 20.0%
!!! AGTTATAAATATTGTTTCTC+TGG + tig0023193:49568-49587 None:intergenic 20.0%
!! AATAACATTAAATGGTAATC+TGG + tig0023193:49737-49756 None:intergenic 20.0%
! AACTTAGAAAAGAAAACCTA+GGG - tig0023193:47781-47800 Msa1406150:three_prime_UTR 25.0%
!! AGAAGAAAAAAGAGGATTTT+AGG - tig0023193:47837-47856 Msa1406150:three_prime_UTR 25.0%
!! GAAGAAAAAAGAGGATTTTA+GGG - tig0023193:47838-47857 Msa1406150:three_prime_UTR 25.0%
!!! ACTTGGATTTCTTTTTTGTT+CGG + tig0023193:47910-47929 None:intergenic 25.0%
!!! GGAATTTTTAAGAAAACCTA+CGG + tig0023193:48646-48665 None:intergenic 25.0%
!! GTTTTCTTAAAAATTCCTCT+AGG - tig0023193:48649-48668 Msa1406150:intron 25.0%
!!! GCAATTTTTAAGAAAACCTA+CGG + tig0023193:48810-48829 None:intergenic 25.0%
!! GTTTTCTTAAAAATTGCTCT+AGG - tig0023193:48813-48832 Msa1406150:intron 25.0%
!!! CCAATTTTAAAGAAAAGCTA+TGG + tig0023193:49182-49201 None:intergenic 25.0%
!!! CCATAGCTTTTCTTTAAAAT+TGG - tig0023193:49179-49198 Msa1406150:CDS 25.0%
! AACTTAGAAAAGAAAACCTA+GGG - tig0023193:47781-47800 Msa1406150:three_prime_UTR 25.0%
!! AGAAGAAAAAAGAGGATTTT+AGG - tig0023193:47837-47856 Msa1406150:three_prime_UTR 25.0%
!! GAAGAAAAAAGAGGATTTTA+GGG - tig0023193:47838-47857 Msa1406150:three_prime_UTR 25.0%
!!! ACTTGGATTTCTTTTTTGTT+CGG + tig0023193:47910-47929 None:intergenic 25.0%
!!! GGAATTTTTAAGAAAACCTA+CGG + tig0023193:48646-48665 None:intergenic 25.0%
!! GTTTTCTTAAAAATTCCTCT+AGG - tig0023193:48649-48668 Msa1406150:intron 25.0%
!!! GCAATTTTTAAGAAAACCTA+CGG + tig0023193:48810-48829 None:intergenic 25.0%
!! GTTTTCTTAAAAATTGCTCT+AGG - tig0023193:48813-48832 Msa1406150:intron 25.0%
!!! CCAATTTTAAAGAAAAGCTA+TGG + tig0023193:49182-49201 None:intergenic 25.0%
!!! CCATAGCTTTTCTTTAAAAT+TGG - tig0023193:49179-49198 Msa1406150:CDS 25.0%
CAACTTAGAAAAGAAAACCT+AGG - tig0023193:47780-47799 Msa1406150:three_prime_UTR 30.0%
AAGAGAAGAGAAGAAAAAAG+AGG - tig0023193:47829-47848 Msa1406150:three_prime_UTR 30.0%
TTATGATCAGATTTATCGTC+AGG - tig0023193:48060-48079 Msa1406150:CDS 30.0%
!! GCAATTTTTGAGAAAACCTA+CGG + tig0023193:48482-48501 None:intergenic 30.0%
! GTTTTCTCAAAAATTGCTCT+AGG - tig0023193:48485-48504 Msa1406150:intron 30.0%
GAATAGAAGTGGTATACTTA+TGG + tig0023193:49101-49120 None:intergenic 30.0%
AGATGAAAAGTGAATAGAAG+TGG + tig0023193:49112-49131 None:intergenic 30.0%
CTTCTTACTAATACACCAAA+TGG - tig0023193:49206-49225 Msa1406150:CDS 30.0%
CGACACAAAATAATAAACCT+AGG + tig0023193:49490-49509 None:intergenic 30.0%
TTGAATAAATAGCTTGACCA+AGG + tig0023193:49670-49689 None:intergenic 30.0%
CAACTTAGAAAAGAAAACCT+AGG - tig0023193:47780-47799 Msa1406150:three_prime_UTR 30.0%
AAGAGAAGAGAAGAAAAAAG+AGG - tig0023193:47829-47848 Msa1406150:three_prime_UTR 30.0%
TTATGATCAGATTTATCGTC+AGG - tig0023193:48060-48079 Msa1406150:CDS 30.0%
!! GCAATTTTTGAGAAAACCTA+CGG + tig0023193:48482-48501 None:intergenic 30.0%
! GTTTTCTCAAAAATTGCTCT+AGG - tig0023193:48485-48504 Msa1406150:intron 30.0%
GAATAGAAGTGGTATACTTA+TGG + tig0023193:49101-49120 None:intergenic 30.0%
AGATGAAAAGTGAATAGAAG+TGG + tig0023193:49112-49131 None:intergenic 30.0%
CTTCTTACTAATACACCAAA+TGG - tig0023193:49206-49225 Msa1406150:CDS 30.0%
CGACACAAAATAATAAACCT+AGG + tig0023193:49490-49509 None:intergenic 30.0%
TTGAATAAATAGCTTGACCA+AGG + tig0023193:49670-49689 None:intergenic 30.0%
GAATTGAAAGAGAAGAAGAG+AGG + tig0023193:47758-47777 None:intergenic 35.0%
!! CTTCTGAAATTCCTTCGATT+CGG + tig0023193:47812-47831 None:intergenic 35.0%
CAGTTTCTTCTTCTTAGACT+TGG + tig0023193:47927-47946 None:intergenic 35.0%
TTGGAAAGATAGATAGAGAG+AGG + tig0023193:47958-47977 None:intergenic 35.0%
! CATGATGATGATGATGATCT+TGG - tig0023193:48163-48182 Msa1406150:intron 35.0%
TCATCTTCCATCGTATTTGT+AGG - tig0023193:48225-48244 Msa1406150:intron 35.0%
GATAGTACCTACAAATACGA+TGG + tig0023193:48235-48254 None:intergenic 35.0%
!!! CTCTTCTTCTGCTACTTTTT+TGG + tig0023193:48269-48288 None:intergenic 35.0%
CAAAAAAGTAGCAGAAGAAG+AGG - tig0023193:48267-48286 Msa1406150:intron 35.0%
TCTTCCTCAAAAAAAACTGC+AGG - tig0023193:48315-48334 Msa1406150:intron 35.0%
TCTAAATTCACTCTGGTCAT+TGG - tig0023193:48375-48394 Msa1406150:intron 35.0%
! TTTTGTAGTCATGTTTGGGT+AGG + tig0023193:48422-48441 None:intergenic 35.0%
! GCGTTTTTGTAGTCATGTTT+GGG + tig0023193:48426-48445 None:intergenic 35.0%
! AGCGTTTTTGTAGTCATGTT+TGG + tig0023193:48427-48446 None:intergenic 35.0%
!! CGTTGTTTCTTAGTAAGGAA+AGG + tig0023193:48521-48540 None:intergenic 35.0%
!! AAGCACGTTGTTTCTTAGTA+AGG + tig0023193:48526-48545 None:intergenic 35.0%
! TTTTGTAATCTGTATGCAGC+TGG - tig0023193:48569-48588 Msa1406150:intron 35.0%
! GAAAAAGCGTATCATCTTCA+TGG + tig0023193:48596-48615 None:intergenic 35.0%
!! CGTTGTTTCTTAGTAAGGAA+AGG + tig0023193:48521-48540 None:intergenic 35.0%
!! AAGCACGTTGTTTCTTAGTA+AGG + tig0023193:48526-48545 None:intergenic 35.0%
! GAAAAAGCGTATCATCTTCA+TGG + tig0023193:48596-48615 None:intergenic 35.0%
AATAGGAGATAGGTTTATGC+AGG + tig0023193:49006-49025 None:intergenic 35.0%
CTGTTTCATGAATAGGAGAT+AGG + tig0023193:49016-49035 None:intergenic 35.0%
! ATGGATTGACATGAATGTTC+AGG + tig0023193:49082-49101 None:intergenic 35.0%
TGAAAATGATATGAAGCCCT+TGG - tig0023193:49343-49362 Msa1406150:intron 35.0%
!!! ATAAAGCTGCTTTTCAGGAA+GGG - tig0023193:49429-49448 Msa1406150:intron 35.0%
! GTCCAATATCTAGAGTAGTT+GGG + tig0023193:49468-49487 None:intergenic 35.0%
ATAGTACACTTGCAACAACT+AGG + tig0023193:49518-49537 None:intergenic 35.0%
GTTGCAAGTGTACTATGAAA+TGG - tig0023193:49522-49541 Msa1406150:intron 35.0%
! GTGTACTATGAAATGGCTTT+TGG - tig0023193:49529-49548 Msa1406150:intron 35.0%
TAGTATGCATAGGAAGTAGA+AGG + tig0023193:49621-49640 None:intergenic 35.0%
TTCTACTTCCTATGCATACT+AGG - tig0023193:49620-49639 Msa1406150:five_prime_UTR 35.0%
GAATTGAAAGAGAAGAAGAG+AGG + tig0023193:47758-47777 None:intergenic 35.0%
!! CTTCTGAAATTCCTTCGATT+CGG + tig0023193:47812-47831 None:intergenic 35.0%
CAGTTTCTTCTTCTTAGACT+TGG + tig0023193:47927-47946 None:intergenic 35.0%
TTGGAAAGATAGATAGAGAG+AGG + tig0023193:47958-47977 None:intergenic 35.0%
! CATGATGATGATGATGATCT+TGG - tig0023193:48163-48182 Msa1406150:intron 35.0%
TCATCTTCCATCGTATTTGT+AGG - tig0023193:48225-48244 Msa1406150:intron 35.0%
GATAGTACCTACAAATACGA+TGG + tig0023193:48235-48254 None:intergenic 35.0%
!!! CTCTTCTTCTGCTACTTTTT+TGG + tig0023193:48269-48288 None:intergenic 35.0%
CAAAAAAGTAGCAGAAGAAG+AGG - tig0023193:48267-48286 Msa1406150:intron 35.0%
TCTTCCTCAAAAAAAACTGC+AGG - tig0023193:48315-48334 Msa1406150:intron 35.0%
TCTAAATTCACTCTGGTCAT+TGG - tig0023193:48375-48394 Msa1406150:intron 35.0%
! TTTTGTAGTCATGTTTGGGT+AGG + tig0023193:48422-48441 None:intergenic 35.0%
! GCGTTTTTGTAGTCATGTTT+GGG + tig0023193:48426-48445 None:intergenic 35.0%
! AGCGTTTTTGTAGTCATGTT+TGG + tig0023193:48427-48446 None:intergenic 35.0%
!! CGTTGTTTCTTAGTAAGGAA+AGG + tig0023193:48521-48540 None:intergenic 35.0%
!! AAGCACGTTGTTTCTTAGTA+AGG + tig0023193:48526-48545 None:intergenic 35.0%
! TTTTGTAATCTGTATGCAGC+TGG - tig0023193:48569-48588 Msa1406150:intron 35.0%
! GAAAAAGCGTATCATCTTCA+TGG + tig0023193:48596-48615 None:intergenic 35.0%
!! CGTTGTTTCTTAGTAAGGAA+AGG + tig0023193:48521-48540 None:intergenic 35.0%
!! AAGCACGTTGTTTCTTAGTA+AGG + tig0023193:48526-48545 None:intergenic 35.0%
! GAAAAAGCGTATCATCTTCA+TGG + tig0023193:48596-48615 None:intergenic 35.0%
AATAGGAGATAGGTTTATGC+AGG + tig0023193:49006-49025 None:intergenic 35.0%
CTGTTTCATGAATAGGAGAT+AGG + tig0023193:49016-49035 None:intergenic 35.0%
! ATGGATTGACATGAATGTTC+AGG + tig0023193:49082-49101 None:intergenic 35.0%
TGAAAATGATATGAAGCCCT+TGG - tig0023193:49343-49362 Msa1406150:intron 35.0%
!!! ATAAAGCTGCTTTTCAGGAA+GGG - tig0023193:49429-49448 Msa1406150:intron 35.0%
! GTCCAATATCTAGAGTAGTT+GGG + tig0023193:49468-49487 None:intergenic 35.0%
ATAGTACACTTGCAACAACT+AGG + tig0023193:49518-49537 None:intergenic 35.0%
GTTGCAAGTGTACTATGAAA+TGG - tig0023193:49522-49541 Msa1406150:intron 35.0%
! GTGTACTATGAAATGGCTTT+TGG - tig0023193:49529-49548 Msa1406150:intron 35.0%
TAGTATGCATAGGAAGTAGA+AGG + tig0023193:49621-49640 None:intergenic 35.0%
TTCTACTTCCTATGCATACT+AGG - tig0023193:49620-49639 Msa1406150:five_prime_UTR 35.0%
AAGAGAAGAAGAGAGGAGAT+AGG + tig0023193:47751-47770 None:intergenic 40.0%
! ATGATCTTGGCTCTCTGTAT+AGG - tig0023193:48176-48195 Msa1406150:intron 40.0%
! CTCTTGCTCTAAATTCACTC+TGG - tig0023193:48368-48387 Msa1406150:intron 40.0%
AAATCTACAAACACAGCCGT+AGG - tig0023193:48463-48482 Msa1406150:intron 40.0%
AAAGGACAAGACAATCCTAG+AGG + tig0023193:48503-48522 None:intergenic 40.0%
! TTTTGTAACCTGTATGCAGC+TGG - tig0023193:48733-48752 Msa1406150:intron 40.0%
! TTTTGTAATCTGGATGCAGC+TGG - tig0023193:48877-48896 Msa1406150:intron 40.0%
GTGAAGCTGTATCATCTTCA+TGG + tig0023193:48904-48923 None:intergenic 40.0%
! CACTAGGCTGTTTCATGAAT+AGG + tig0023193:49023-49042 None:intergenic 40.0%
AATATCCAGACACACACACT+AGG + tig0023193:49039-49058 None:intergenic 40.0%
ACATGAATGTTCAGGACTTC+AGG + tig0023193:49074-49093 None:intergenic 40.0%
GATAAGAAGACAGTCCTAGA+GGG + tig0023193:49250-49269 None:intergenic 40.0%
!! AACACAAAACGTTAGCCTTG+AGG - tig0023193:49406-49425 Msa1406150:CDS 40.0%
! GAAAAGCAGCTTTATCCTCA+AGG + tig0023193:49424-49443 None:intergenic 40.0%
!!! TGAGGATAAAGCTGCTTTTC+AGG - tig0023193:49424-49443 Msa1406150:CDS 40.0%
!!! GATAAAGCTGCTTTTCAGGA+AGG - tig0023193:49428-49447 Msa1406150:intron 40.0%
!!! TAAAGCTGCTTTTCAGGAAG+GGG - tig0023193:49430-49449 Msa1406150:intron 40.0%
! GGTCCAATATCTAGAGTAGT+TGG + tig0023193:49469-49488 None:intergenic 40.0%
CTACTCTAGATATTGGACCT+AGG - tig0023193:49470-49489 Msa1406150:intron 40.0%
CAACTTCTCCTAGTATGCAT+AGG + tig0023193:49631-49650 None:intergenic 40.0%
CTGCATATCTGACCTTAGTT+AGG - tig0023193:49690-49709 Msa1406150:five_prime_UTR 40.0%
AAGAGAAGAAGAGAGGAGAT+AGG + tig0023193:47751-47770 None:intergenic 40.0%
! ATGATCTTGGCTCTCTGTAT+AGG - tig0023193:48176-48195 Msa1406150:intron 40.0%
! CTCTTGCTCTAAATTCACTC+TGG - tig0023193:48368-48387 Msa1406150:intron 40.0%
AAATCTACAAACACAGCCGT+AGG - tig0023193:48463-48482 Msa1406150:intron 40.0%
AAAGGACAAGACAATCCTAG+AGG + tig0023193:48503-48522 None:intergenic 40.0%
! TTTTGTAACCTGTATGCAGC+TGG - tig0023193:48733-48752 Msa1406150:intron 40.0%
! TTTTGTAATCTGGATGCAGC+TGG - tig0023193:48877-48896 Msa1406150:intron 40.0%
GTGAAGCTGTATCATCTTCA+TGG + tig0023193:48904-48923 None:intergenic 40.0%
! CACTAGGCTGTTTCATGAAT+AGG + tig0023193:49023-49042 None:intergenic 40.0%
AATATCCAGACACACACACT+AGG + tig0023193:49039-49058 None:intergenic 40.0%
ACATGAATGTTCAGGACTTC+AGG + tig0023193:49074-49093 None:intergenic 40.0%
GATAAGAAGACAGTCCTAGA+GGG + tig0023193:49250-49269 None:intergenic 40.0%
!! AACACAAAACGTTAGCCTTG+AGG - tig0023193:49406-49425 Msa1406150:CDS 40.0%
! GAAAAGCAGCTTTATCCTCA+AGG + tig0023193:49424-49443 None:intergenic 40.0%
!!! TGAGGATAAAGCTGCTTTTC+AGG - tig0023193:49424-49443 Msa1406150:CDS 40.0%
!!! GATAAAGCTGCTTTTCAGGA+AGG - tig0023193:49428-49447 Msa1406150:intron 40.0%
!!! TAAAGCTGCTTTTCAGGAAG+GGG - tig0023193:49430-49449 Msa1406150:intron 40.0%
! GGTCCAATATCTAGAGTAGT+TGG + tig0023193:49469-49488 None:intergenic 40.0%
CTACTCTAGATATTGGACCT+AGG - tig0023193:49470-49489 Msa1406150:intron 40.0%
CAACTTCTCCTAGTATGCAT+AGG + tig0023193:49631-49650 None:intergenic 40.0%
CTGCATATCTGACCTTAGTT+AGG - tig0023193:49690-49709 Msa1406150:five_prime_UTR 40.0%
AGAAGAGAGGAGATAGGCAT+AGG + tig0023193:47745-47764 None:intergenic 45.0%
!! CTGAAATTCCTTCGATTCGG+CGG + tig0023193:47809-47828 None:intergenic 45.0%
AAAGCTACAAACACAGCCGT+AGG - tig0023193:48627-48646 Msa1406150:intron 45.0%
AAAGCTACAAACACAGCCGT+AGG - tig0023193:48627-48646 Msa1406150:intron 45.0%
! TATTGTGTTGGCATGTGCAC+AGG + tig0023193:48935-48954 None:intergenic 45.0%
GTGCACATGCCAACACAATA+TGG - tig0023193:48935-48954 Msa1406150:intron 45.0%
CATGCCAACACAATATGGCA+AGG - tig0023193:48940-48959 Msa1406150:intron 45.0%
ACCTCCTTGCCATATTGTGT+TGG + tig0023193:48947-48966 None:intergenic 45.0%
GGATAAGAAGACAGTCCTAG+AGG + tig0023193:49251-49270 None:intergenic 45.0%
CGCCCAACTACTCTAGATAT+TGG - tig0023193:49463-49482 Msa1406150:intron 45.0%
GTGTTAGCATGTGCATACCT+TGG - tig0023193:49650-49669 Msa1406150:five_prime_UTR 45.0%
TGACCTTAGTTAGGCGACAT+TGG - tig0023193:49699-49718 Msa1406150:five_prime_UTR 45.0%
TGACCAATGTCGCCTAACTA+AGG + tig0023193:49705-49724 None:intergenic 45.0%
AGAAGAGAGGAGATAGGCAT+AGG + tig0023193:47745-47764 None:intergenic 45.0%
!! CTGAAATTCCTTCGATTCGG+CGG + tig0023193:47809-47828 None:intergenic 45.0%
AAAGCTACAAACACAGCCGT+AGG - tig0023193:48627-48646 Msa1406150:intron 45.0%
AAAGCTACAAACACAGCCGT+AGG - tig0023193:48627-48646 Msa1406150:intron 45.0%
! TATTGTGTTGGCATGTGCAC+AGG + tig0023193:48935-48954 None:intergenic 45.0%
GTGCACATGCCAACACAATA+TGG - tig0023193:48935-48954 Msa1406150:intron 45.0%
CATGCCAACACAATATGGCA+AGG - tig0023193:48940-48959 Msa1406150:intron 45.0%
ACCTCCTTGCCATATTGTGT+TGG + tig0023193:48947-48966 None:intergenic 45.0%
GGATAAGAAGACAGTCCTAG+AGG + tig0023193:49251-49270 None:intergenic 45.0%
CGCCCAACTACTCTAGATAT+TGG - tig0023193:49463-49482 Msa1406150:intron 45.0%
GTGTTAGCATGTGCATACCT+TGG - tig0023193:49650-49669 Msa1406150:five_prime_UTR 45.0%
TGACCTTAGTTAGGCGACAT+TGG - tig0023193:49699-49718 Msa1406150:five_prime_UTR 45.0%
TGACCAATGTCGCCTAACTA+AGG + tig0023193:49705-49724 None:intergenic 45.0%
!! TACAAAATAAAAATAAAAAA+TGG + tig0023193:48017-48036 None:intergenic 5.0%
!! TACAAAATAAAAATAAAAAA+TGG + tig0023193:48017-48036 None:intergenic 5.0%
! ATAGGCTGACTGGTTGAGAG+AGG + tig0023193:47727-47746 None:intergenic 50.0%
AGCCTCTTCAGCTAATCGAC+GGG + tig0023193:48296-48315 None:intergenic 50.0%
GAGCCTCTTCAGCTAATCGA+CGG + tig0023193:48297-48316 None:intergenic 50.0%
!!! CGGGCCTGCAGTTTTTTTTG+AGG + tig0023193:48322-48341 None:intergenic 50.0%
! TGCTGCCTCTTTTGCTTTGC+GGG + tig0023193:48341-48360 None:intergenic 50.0%
! CTGCTGCCTCTTTTGCTTTG+CGG + tig0023193:48342-48361 None:intergenic 50.0%
TTCATGGTCCAGCTGCATAC+AGG + tig0023193:48580-48599 None:intergenic 50.0%
! GGCATGTGCACAGGTTGAAT+TGG + tig0023193:48926-48945 None:intergenic 50.0%
GCCAACACAATATGGCAAGG+AGG - tig0023193:48943-48962 Msa1406150:intron 50.0%
AACAGCCTAGTGTGTGTGTC+TGG - tig0023193:49031-49050 Msa1406150:CDS 50.0%
CACCAAATGGAGAGAGCCTT+AGG - tig0023193:49219-49238 Msa1406150:intron 50.0%
TGCCTAAGGCTCTCTCCATT+TGG + tig0023193:49224-49243 None:intergenic 50.0%
TGTTGAGCTCATCCAACAGC+TGG + tig0023193:49382-49401 None:intergenic 50.0%
!! GCTTTTCAGGAAGGGGCTAT+TGG - tig0023193:49437-49456 Msa1406150:intron 50.0%
! TAGGCTGACTGGTTGAGAGA+GGG + tig0023193:47726-47745 None:intergenic 50.0%
! ATAGGCTGACTGGTTGAGAG+AGG + tig0023193:47727-47746 None:intergenic 50.0%
AGCCTCTTCAGCTAATCGAC+GGG + tig0023193:48296-48315 None:intergenic 50.0%
GAGCCTCTTCAGCTAATCGA+CGG + tig0023193:48297-48316 None:intergenic 50.0%
!!! CGGGCCTGCAGTTTTTTTTG+AGG + tig0023193:48322-48341 None:intergenic 50.0%
! TGCTGCCTCTTTTGCTTTGC+GGG + tig0023193:48341-48360 None:intergenic 50.0%
! CTGCTGCCTCTTTTGCTTTG+CGG + tig0023193:48342-48361 None:intergenic 50.0%
TTCATGGTCCAGCTGCATAC+AGG + tig0023193:48580-48599 None:intergenic 50.0%
! GGCATGTGCACAGGTTGAAT+TGG + tig0023193:48926-48945 None:intergenic 50.0%
GCCAACACAATATGGCAAGG+AGG - tig0023193:48943-48962 Msa1406150:intron 50.0%
AACAGCCTAGTGTGTGTGTC+TGG - tig0023193:49031-49050 Msa1406150:CDS 50.0%
CACCAAATGGAGAGAGCCTT+AGG - tig0023193:49219-49238 Msa1406150:intron 50.0%
TGCCTAAGGCTCTCTCCATT+TGG + tig0023193:49224-49243 None:intergenic 50.0%
TGTTGAGCTCATCCAACAGC+TGG + tig0023193:49382-49401 None:intergenic 50.0%
!! GCTTTTCAGGAAGGGGCTAT+TGG - tig0023193:49437-49456 Msa1406150:intron 50.0%
GGAGATAGGCATAGGCTGAC+TGG + tig0023193:47737-47756 None:intergenic 55.0%
TCCATTCCCGTTCGTTCCCA+TGG - tig0023193:47868-47887 Msa1406150:three_prime_UTR 55.0%
CCATTCCCGTTCGTTCCCAT+GGG - tig0023193:47869-47888 Msa1406150:three_prime_UTR 55.0%
! CTTTTGCTGCCTCCACCCAT+GGG + tig0023193:47887-47906 None:intergenic 55.0%
! AGCCCGTCGATTAGCTGAAG+AGG - tig0023193:48291-48310 Msa1406150:intron 55.0%
AGCCTTAGGCAATGCCCTCT+AGG - tig0023193:49233-49252 Msa1406150:intron 55.0%
!! GTCCTAGAGGGCATTGCCTA+AGG + tig0023193:49238-49257 None:intergenic 55.0%
GGAGATAGGCATAGGCTGAC+TGG + tig0023193:47737-47756 None:intergenic 55.0%
TCCATTCCCGTTCGTTCCCA+TGG - tig0023193:47868-47887 Msa1406150:three_prime_UTR 55.0%
CCATTCCCGTTCGTTCCCAT+GGG - tig0023193:47869-47888 Msa1406150:three_prime_UTR 55.0%
! CTTTTGCTGCCTCCACCCAT+GGG + tig0023193:47887-47906 None:intergenic 55.0%
! AGCCCGTCGATTAGCTGAAG+AGG - tig0023193:48291-48310 Msa1406150:intron 55.0%
AGCCTTAGGCAATGCCCTCT+AGG - tig0023193:49233-49252 Msa1406150:intron 55.0%
!! GTCCTAGAGGGCATTGCCTA+AGG + tig0023193:49238-49257 None:intergenic 55.0%
!! CTTCGATTCGGCGGAACCCT+AGG + tig0023193:47800-47819 None:intergenic 60.0%
CTAGGGTTCCGCCGAATCGA+AGG - tig0023193:47798-47817 Msa1406150:three_prime_UTR 60.0%
CCCATGGGAACGAACGGGAA+TGG + tig0023193:47872-47891 None:intergenic 60.0%
TTCCCGTTCGTTCCCATGGG+TGG - tig0023193:47872-47891 Msa1406150:three_prime_UTR 60.0%
CTCCACCCATGGGAACGAAC+GGG + tig0023193:47877-47896 None:intergenic 60.0%
CCTCCACCCATGGGAACGAA+CGG + tig0023193:47878-47897 None:intergenic 60.0%
! GCTTTTGCTGCCTCCACCCA+TGG + tig0023193:47888-47907 None:intergenic 60.0%
GCAGGCCCGCAAAGCAAAAG+AGG - tig0023193:48333-48352 Msa1406150:intron 60.0%
TGGCTGCTGCTGGCATCCAA+GGG + tig0023193:49362-49381 None:intergenic 60.0%
ATCCAACAGCTGGCTGCTGC+TGG + tig0023193:49372-49391 None:intergenic 60.0%
!! CTTCGATTCGGCGGAACCCT+AGG + tig0023193:47800-47819 None:intergenic 60.0%
CTAGGGTTCCGCCGAATCGA+AGG - tig0023193:47798-47817 Msa1406150:three_prime_UTR 60.0%
CCCATGGGAACGAACGGGAA+TGG + tig0023193:47872-47891 None:intergenic 60.0%
TTCCCGTTCGTTCCCATGGG+TGG - tig0023193:47872-47891 Msa1406150:three_prime_UTR 60.0%
CTCCACCCATGGGAACGAAC+GGG + tig0023193:47877-47896 None:intergenic 60.0%
CCTCCACCCATGGGAACGAA+CGG + tig0023193:47878-47897 None:intergenic 60.0%
! GCTTTTGCTGCCTCCACCCA+TGG + tig0023193:47888-47907 None:intergenic 60.0%
GCAGGCCCGCAAAGCAAAAG+AGG - tig0023193:48333-48352 Msa1406150:intron 60.0%
TGGCTGCTGCTGGCATCCAA+GGG + tig0023193:49362-49381 None:intergenic 60.0%
ATCCAACAGCTGGCTGCTGC+TGG + tig0023193:49372-49391 None:intergenic 60.0%
CCGTTCGTTCCCATGGGTGG+AGG - tig0023193:47875-47894 Msa1406150:three_prime_UTR 65.0%
CTGGCTGCTGCTGGCATCCA+AGG + tig0023193:49363-49382 None:intergenic 65.0%
TGCCAGCAGCAGCCAGCTGT+TGG - tig0023193:49367-49386 Msa1406150:intron 65.0%
CCGTTCGTTCCCATGGGTGG+AGG - tig0023193:47875-47894 Msa1406150:three_prime_UTR 65.0%
CTGGCTGCTGCTGGCATCCA+AGG + tig0023193:49363-49382 None:intergenic 65.0%
TGCCAGCAGCAGCCAGCTGT+TGG - tig0023193:49367-49386 Msa1406150:intron 65.0%
Chromosome Type Strat End Strand Name
tig0023193 gene 47724 49759 47724 ID=Msa1406150;Name=Msa1406150
tig0023193 mRNA 47724 49759 47724 ID=Msa1406150-mRNA-1;Parent=Msa1406150;Name=Msa1406150-mRNA-1;_AED=0.02;_eAED=0.02;_QI=163|0.83|0.85|1|1|1|7|281|221
tig0023193 exon 47724 48148 47724 ID=Msa1406150-mRNA-1:exon:24429;Parent=Msa1406150-mRNA-1
tig0023193 exon 48519 48587 48519 ID=Msa1406150-mRNA-1:exon:24428;Parent=Msa1406150-mRNA-1
tig0023193 exon 48675 48731 48675 ID=Msa1406150-mRNA-1:exon:24427;Parent=Msa1406150-mRNA-1
tig0023193 exon 48839 48895 48839 ID=Msa1406150-mRNA-1:exon:24426;Parent=Msa1406150-mRNA-1
tig0023193 exon 49003 49236 49003 ID=Msa1406150-mRNA-1:exon:24425;Parent=Msa1406150-mRNA-1
tig0023193 exon 49402 49445 49402 ID=Msa1406150-mRNA-1:exon:24424;Parent=Msa1406150-mRNA-1
tig0023193 exon 49536 49759 49536 ID=Msa1406150-mRNA-1:exon:24423;Parent=Msa1406150-mRNA-1
tig0023193 five_prime_UTR 49597 49759 49597 ID=Msa1406150-mRNA-1:five_prime_utr;Parent=Msa1406150-mRNA-1
tig0023193 CDS 49536 49596 49536 ID=Msa1406150-mRNA-1:cds;Parent=Msa1406150-mRNA-1
tig0023193 CDS 49402 49445 49402 ID=Msa1406150-mRNA-1:cds;Parent=Msa1406150-mRNA-1
tig0023193 CDS 49003 49236 49003 ID=Msa1406150-mRNA-1:cds;Parent=Msa1406150-mRNA-1
tig0023193 CDS 48839 48895 48839 ID=Msa1406150-mRNA-1:cds;Parent=Msa1406150-mRNA-1
tig0023193 CDS 48675 48731 48675 ID=Msa1406150-mRNA-1:cds;Parent=Msa1406150-mRNA-1
tig0023193 CDS 48519 48587 48519 ID=Msa1406150-mRNA-1:cds;Parent=Msa1406150-mRNA-1
tig0023193 CDS 48005 48148 48005 ID=Msa1406150-mRNA-1:cds;Parent=Msa1406150-mRNA-1
tig0023193 three_prime_UTR 47724 48004 47724 ID=Msa1406150-mRNA-1:three_prime_utr;Parent=Msa1406150-mRNA-1
Gene Sequence

>Msa1406150

ATGGGTGGAGGCAGCAAAAGCCGAACAAAAAAGAAATCCAAGTCTAAGAAGAAGAAACTGACATCTTCTGAACGTCAATCTATTTATGATCAGATTTATCGTCAGGTACTATCTGATATACAAGCCAAAAAAGTAGCAGAAGAAGAGGAAGCCCGTCGATTAGCTGAAGAGGCTCTTCCTCAAAAAAAACTGCAGGCCCGCAAAGCAAAAGAGGCAGCAGAAAAGACTCTTGCTCTAAATTCACTCTGGTCATTGGCTGATGCAATGCGCTGTCGTTCCTACCCAAACATGACTACAAAAACGCTTTTTCTAAAACTTGCAAAATCTACAAACACAGCCCTGGACCATGAAGATGATACGCTTTTTCGAAAACTTGCAAAAGCTACAAACACAGCCCTGGACCATGAAGATGATACGCTTTTTCGAAAACTTGCAAAAGCTACAAACACAGCCCTGGACCATGAAGATGATACAGCTTCACCAATTCAACCTGTGCACATGCCAACACAATATGGCAAGGAGGTCAACTATGAAAATGATATGAAGCCCTTGGATGCCAGCAGCAGCCAGCTGTTGGATGAGCTCAACAAGTCAACACAAAACGTTAGCCTTGAGGATAAAGCTGCTTTTCAGGAAGGGGCTATTGGCAGCGCCCAACTACTCTAG
Protein sequence

>Msa1406150

MGGGSKSRTKKKSKSKKKKLTSSERQSIYDQIYRQVLSDIQAKKVAEEEEARRLAEEALPQKKLQARKAKEAAEKTLALNSLWSLADAMRCRSYPNMTTKTLFLKLAKSTNTALDHEDDTLFRKLAKATNTALDHEDDTLFRKLAKATNTALDHEDDTASPIQPVHMPTQYGKEVNYENDMKPLDASSSQLLDELNKSTQNVSLEDKAAFQEGAIGSAQLL*