AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1406980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 10 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCGACTTCCTTTAATTTCT+TGG 0.140936 tig0023248:+44016 Msa1406980:five_prime_UTR
TATTTCCTTTCTTACTTTGT+CGG 0.303938 tig0023248:+47586 Msa1406980:three_prime_UTR
CACACAAACCAAGAAATTAA+AGG 0.317020 tig0023248:-44024 None:intergenic
ATTGGAGAGAAGTTTGATAA+TGG 0.340256 tig0023248:+44270 Msa1406980:exon
TTGAAAAGAAACCGAGCATT+AGG 0.357779 tig0023248:-47704 None:intergenic
CAGAAATTCAAAAGGGATGA+AGG 0.374176 tig0023248:+44068 Msa1406980:five_prime_UTR
GTTGATGATGAGTGCTGAAA+AGG 0.499834 tig0023248:+44303 Msa1406980:CDS
ATAAACCGACAAAGTAAGAA+AGG 0.510637 tig0023248:-47591 None:intergenic
ATATGAACTTTCCTAATGCT+CGG 0.514200 tig0023248:+47693 Msa1406980:three_prime_UTR
AAATTCATGGTATCTATAAG+AGG 0.523952 tig0023248:+44121 Msa1406980:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTGATACAAAATATAA+TGG - tig0023248:44856-44875 None:intergenic 10.0%
!!! TTTATATATATTTTTGTTAC+AGG + tig0023248:44988-45007 Msa1406980:intron 10.0%
!!! TTATTTGAAGTTTAATTAAA+TGG + tig0023248:45076-45095 Msa1406980:intron 10.0%
!! CAATTTATCAATAAAATTTA+AGG + tig0023248:45496-45515 Msa1406980:intron 10.0%
!! AATTTATGCAAAAAAAGTAA+GGG - tig0023248:44106-44125 None:intergenic 15.0%
!!! AAACATTGATTGATTTTAAT+GGG + tig0023248:44151-44170 Msa1406980:intron 15.0%
!! TGAAAGTTAAATTCAAAAAT+TGG + tig0023248:44252-44271 Msa1406980:five_prime_UTR 15.0%
!! GTAAAAAAAAATAGACAAAA+CGG - tig0023248:45398-45417 None:intergenic 15.0%
!!! ATTTTCATCTCTATTAATAA+TGG + tig0023248:45433-45452 Msa1406980:intron 15.0%
!!! ATAAAATTTAAGGTTGTTTA+AGG + tig0023248:45506-45525 Msa1406980:intron 15.0%
!!! TTCTTTTCATTTCTAATTTA+TGG - tig0023248:45644-45663 None:intergenic 15.0%
!! AAATAAAATTATTCAACATG+TGG - tig0023248:45907-45926 None:intergenic 15.0%
!!! ATGTTTTTTTTACTTTTGAT+TGG + tig0023248:46260-46279 Msa1406980:intron 15.0%
!! CTAACTATTAATGATAATAA+GGG - tig0023248:46489-46508 None:intergenic 15.0%
!! ACTAACTATTAATGATAATA+AGG - tig0023248:46490-46509 None:intergenic 15.0%
!! TTTATATATAGATGTTGATA+TGG - tig0023248:47429-47448 None:intergenic 15.0%
!! GAATTTATGCAAAAAAAGTA+AGG - tig0023248:44107-44126 None:intergenic 20.0%
!!! ACTTTTTTTGCATAAATTCA+TGG + tig0023248:44108-44127 Msa1406980:five_prime_UTR 20.0%
!!! GAAACATTGATTGATTTTAA+TGG + tig0023248:44150-44169 Msa1406980:intron 20.0%
!! AATTCTAAACACAAACAAAT+AGG + tig0023248:44328-44347 Msa1406980:intron 20.0%
!!! AGAAGTAATTTGCTAATTTT+TGG + tig0023248:44536-44555 Msa1406980:intron 20.0%
!! ATTGAAACAAAATGAATGAA+AGG - tig0023248:44821-44840 None:intergenic 20.0%
!!! TTTCAGTGAAAATAAACTTT+TGG - tig0023248:44899-44918 None:intergenic 20.0%
!!! AATTAAATGGTTTTATGATG+TGG + tig0023248:45089-45108 Msa1406980:intron 20.0%
!! TTCAGTAATATACAAATTTC+AGG + tig0023248:45191-45210 Msa1406980:intron 20.0%
!!! TAATATACAAATTTCAGGTT+AGG + tig0023248:45196-45215 Msa1406980:intron 20.0%
!!! TATATCAAAGATTTTGTAGA+AGG + tig0023248:45342-45361 Msa1406980:intron 20.0%
!! CACTATTAATTAATAACCAA+GGG - tig0023248:45839-45858 None:intergenic 20.0%
!! TCACTATTAATTAATAACCA+AGG - tig0023248:45840-45859 None:intergenic 20.0%
!!! AGACTTTACTATTTTTCATT+TGG + tig0023248:45984-46003 Msa1406980:intron 20.0%
!!! GACTTTACTATTTTTCATTT+GGG + tig0023248:45985-46004 Msa1406980:intron 20.0%
!! TTATTGTTAAACTGAAAAAG+AGG - tig0023248:46048-46067 None:intergenic 20.0%
!! AAATGAATATAATCATAGTC+CGG - tig0023248:46145-46164 None:intergenic 20.0%
!!! TTTAAAAAGTATTGACATTC+TGG + tig0023248:46368-46387 Msa1406980:intron 20.0%
!!! ACATTTTACATTTCTAAGAA+CGG + tig0023248:46463-46482 Msa1406980:intron 20.0%
!!! TGTTTTTAGTTTAAGTTGTT+GGG + tig0023248:46616-46635 Msa1406980:intron 20.0%
!! TAAAAGAATCAGACAATAAA+AGG - tig0023248:46859-46878 None:intergenic 20.0%
!! TATTCAAGATATAAACCATA+AGG + tig0023248:46923-46942 Msa1406980:intron 20.0%
!! TATAAAACAAAGTTACCTTA+TGG - tig0023248:46941-46960 None:intergenic 20.0%
!! TTATACTTAAATGTCCAAAA+AGG - tig0023248:47052-47071 None:intergenic 20.0%
!! TATGAACTATTAATATCCAA+AGG - tig0023248:47505-47524 None:intergenic 20.0%
!!! TTATTATAATCGAATTTTGC+AGG + tig0023248:47541-47560 Msa1406980:intron 20.0%
!! GAAAAATACAAAAATTACAG+AGG - tig0023248:47643-47662 None:intergenic 20.0%
!! ATTTCTGAAAAAGCAATGAA+AGG - tig0023248:44055-44074 None:intergenic 25.0%
!!! TGCTTTTTCAGAAATTCAAA+AGG + tig0023248:44060-44079 Msa1406980:five_prime_UTR 25.0%
!!! GCTTTTTCAGAAATTCAAAA+GGG + tig0023248:44061-44080 Msa1406980:five_prime_UTR 25.0%
! AAATTCATGGTATCTATAAG+AGG + tig0023248:44121-44140 Msa1406980:five_prime_UTR 25.0%
!!! GGACATTGATTGATTTTAAT+GGG + tig0023248:44172-44191 Msa1406980:intron 25.0%
!! AAAACTTAAGTCTCTTTTTG+AGG + tig0023248:44384-44403 Msa1406980:intron 25.0%
!! AAACTTAAGTCTCTTTTTGA+GGG + tig0023248:44385-44404 Msa1406980:intron 25.0%
! AGACTAGATAATTCTTTCTT+TGG + tig0023248:44622-44641 Msa1406980:intron 25.0%
! GATAATTCTTTCTTTGGTAA+AGG + tig0023248:44628-44647 Msa1406980:intron 25.0%
! TCACTGAAATTGTTTAATGT+AGG + tig0023248:44910-44929 Msa1406980:intron 25.0%
!! TTTTCATTGTAGTTGATCAA+AGG + tig0023248:45253-45272 Msa1406980:intron 25.0%
! AAATCTTTGATATAGCATCA+TGG - tig0023248:45336-45355 None:intergenic 25.0%
! TGCGAAGAAAATTTAGAAAA+TGG - tig0023248:45368-45387 None:intergenic 25.0%
!! GAAAAGAATTTTATCTTGAG+AGG + tig0023248:45656-45675 Msa1406980:intron 25.0%
! TATTTGTCTCTCTTTACTAT+AGG + tig0023248:45922-45941 Msa1406980:intron 25.0%
! ATTTGTCTCTCTTTACTATA+GGG + tig0023248:45923-45942 Msa1406980:intron 25.0%
! CACAAATATGGTTAGAAAAT+AGG - tig0023248:46072-46091 None:intergenic 25.0%
!! CTATTTTCTAACCATATTTG+TGG + tig0023248:46070-46089 Msa1406980:intron 25.0%
!! CTAAATGAGATAATTTTTGC+CGG + tig0023248:46123-46142 Msa1406980:intron 25.0%
!!! CTGTTTTTAGTTTAAGTTGT+TGG + tig0023248:46615-46634 Msa1406980:intron 25.0%
!!! GTTTTTAGTTTAAGTTGTTG+GGG + tig0023248:46617-46636 Msa1406980:intron 25.0%
! ATTGGATACCATAACTAAAT+AGG - tig0023248:46649-46668 None:intergenic 25.0%
! ATAAAAGGCAATGTCAATTT+CGG - tig0023248:46844-46863 None:intergenic 25.0%
! ATACCATGACATAATAACAT+TGG + tig0023248:46971-46990 Msa1406980:intron 25.0%
! ATAACATTGGAGATAATTAG+TGG + tig0023248:46984-47003 Msa1406980:intron 25.0%
! TAACATTGGAGATAATTAGT+GGG + tig0023248:46985-47004 Msa1406980:intron 25.0%
! AATTAGTGGGAATAAGATTA+AGG + tig0023248:46998-47017 Msa1406980:intron 25.0%
! ATTAGTGGGAATAAGATTAA+GGG + tig0023248:46999-47018 Msa1406980:intron 25.0%
!! TATCAGTGTACACATTTTAT+TGG - tig0023248:47339-47358 None:intergenic 25.0%
! ACTATTAATATCCAAAGGAA+AGG - tig0023248:47500-47519 None:intergenic 25.0%
! TATTTCCTTTCTTACTTTGT+CGG + tig0023248:47586-47605 Msa1406980:three_prime_UTR 25.0%
! ATACAAAAATTACAGAGGAA+AGG - tig0023248:47638-47657 None:intergenic 25.0%
CACACAAACCAAGAAATTAA+AGG - tig0023248:44027-44046 None:intergenic 30.0%
ATGGTATCTATAAGAGGTAA+TGG + tig0023248:44127-44146 Msa1406980:intron 30.0%
TGGTATCTATAAGAGGTAAT+GGG + tig0023248:44128-44147 Msa1406980:intron 30.0%
!! GGGACATTGATTGATTTTAA+TGG + tig0023248:44171-44190 Msa1406980:intron 30.0%
ATTGGAGAGAAGTTTGATAA+TGG + tig0023248:44270-44289 Msa1406980:exon 30.0%
CTTCACAAAAGAAGCTAATA+GGG - tig0023248:44667-44686 None:intergenic 30.0%
ACTTCACAAAAGAAGCTAAT+AGG - tig0023248:44668-44687 None:intergenic 30.0%
! AGGTTTCGCTTTAAGTTATT+TGG + tig0023248:44930-44949 Msa1406980:intron 30.0%
! TCAATTCTTTTCAGTTGTGA+TGG + tig0023248:45012-45031 Msa1406980:CDS 30.0%
!!! TAAATGGTTTTATGATGTGG+AGG + tig0023248:45092-45111 Msa1406980:intron 30.0%
ATAAATTGATCCTGCAACAA+AGG - tig0023248:45142-45161 None:intergenic 30.0%
AGAAATGAACCAAAAGACAA+AGG - tig0023248:45224-45243 None:intergenic 30.0%
!!! ATCAAAGGTGTGCATTTTTT+TGG + tig0023248:45268-45287 Msa1406980:intron 30.0%
! TTTCTAAATTTTCTTCGCAC+TGG + tig0023248:45369-45388 Msa1406980:intron 30.0%
ATAGATCAGATCCACAAATA+TGG - tig0023248:46084-46103 None:intergenic 30.0%
TTCCAGAATATGTAATGAGT+GGG - tig0023248:46524-46543 None:intergenic 30.0%
! CATTACATATTCTGGAAGTA+TGG + tig0023248:46527-46546 Msa1406980:intron 30.0%
GAATCATATTAAGAGAGAAG+AGG - tig0023248:46686-46705 None:intergenic 30.0%
CTCTTAATATGATTCTTCGA+AGG + tig0023248:46691-46710 Msa1406980:intron 30.0%
TTCATGGACAATTTCTTTAG+TGG - tig0023248:46788-46807 None:intergenic 30.0%
ACTAAAGAAATTGTCCATGA+AGG + tig0023248:46787-46806 Msa1406980:intron 30.0%
TCTCCAATGTTATTATGTCA+TGG - tig0023248:46977-46996 None:intergenic 30.0%
TTAGTGGGAATAAGATTAAG+GGG + tig0023248:47000-47019 Msa1406980:intron 30.0%
TACCTATTTGAATTGTGTCA+CGG + tig0023248:47086-47105 Msa1406980:intron 30.0%
!! CATTTGATTTGAGGATAAAG+AGG - tig0023248:47274-47293 None:intergenic 30.0%
!! AATGTCACTTGCAAATTTTG+AGG + tig0023248:47290-47309 Msa1406980:intron 30.0%
TCTAGTTAAAGCGTATACAT+AGG - tig0023248:47476-47495 None:intergenic 30.0%
ATAAACCGACAAAGTAAGAA+AGG - tig0023248:47594-47613 None:intergenic 30.0%
!!! CTTTGTCGGTTTATATTTTG+AGG + tig0023248:47600-47619 Msa1406980:three_prime_UTR 30.0%
ATATGAACTTTCCTAATGCT+CGG + tig0023248:47693-47712 Msa1406980:three_prime_UTR 30.0%
! CTCGACTTCCTTTAATTTCT+TGG + tig0023248:44016-44035 Msa1406980:five_prime_UTR 35.0%
CAGAAATTCAAAAGGGATGA+AGG + tig0023248:44068-44087 Msa1406980:five_prime_UTR 35.0%
AAACACAAACAAATAGGCAC+AGG + tig0023248:44334-44353 Msa1406980:intron 35.0%
TTGTTGAGTTACTCTTGTGT+AGG - tig0023248:44474-44493 None:intergenic 35.0%
!! TCTTTTCAGTTGTGATGGAA+AGG + tig0023248:45017-45036 Msa1406980:CDS 35.0%
GATTAAGCATAAAACCAGCA+AGG - tig0023248:45314-45333 None:intergenic 35.0%
!! TAGAAGTACTGCTTGATGTT+TGG + tig0023248:45703-45722 Msa1406980:intron 35.0%
!! GCTTTTCTTTTTTGCAAGAG+AGG + tig0023248:46007-46026 Msa1406980:intron 35.0%
GTCAGTAGTATGTCAACATA+TGG + tig0023248:46231-46250 Msa1406980:intron 35.0%
CTTCCAGAATATGTAATGAG+TGG - tig0023248:46525-46544 None:intergenic 35.0%
GGTCAGTTCCTATTTAGTTA+TGG + tig0023248:46638-46657 Msa1406980:intron 35.0%
GGTATCCAATTCAGTAAATG+AGG + tig0023248:46659-46678 Msa1406980:intron 35.0%
!! AAAGGTTGAGAGAGTGTTAT+GGG - tig0023248:47034-47053 None:intergenic 35.0%
!! AAAAGGTTGAGAGAGTGTTA+TGG - tig0023248:47035-47054 None:intergenic 35.0%
! TAACACTCTCTCAACCTTTT+TGG + tig0023248:47035-47054 Msa1406980:intron 35.0%
TGTGTATCTAGTAGTTGCAA+TGG - tig0023248:47145-47164 None:intergenic 35.0%
ATCGTATGACTTGAGAAGAA+AGG - tig0023248:47183-47202 None:intergenic 35.0%
!! TGCAAGTGACATTTGATTTG+AGG - tig0023248:47283-47302 None:intergenic 35.0%
GAAGCTAAAACGATATTCAC+AGG - tig0023248:47407-47426 None:intergenic 35.0%
GTTCATGAGAAACGAACATT+TGG - tig0023248:47454-47473 None:intergenic 35.0%
TTTAACTAGAGCCTTTCCTT+TGG + tig0023248:47486-47505 Msa1406980:intron 35.0%
TTGAAAAGAAACCGAGCATT+AGG - tig0023248:47707-47726 None:intergenic 35.0%
! CTGCACTTGGTAACAACAAA+AGG - tig0023248:44210-44229 None:intergenic 40.0%
!!! CTTTTGTTGTTACCAAGTGC+AGG + tig0023248:44208-44227 Msa1406980:intron 40.0%
!! GTTGATGATGAGTGCTGAAA+AGG + tig0023248:44303-44322 Msa1406980:CDS 40.0%
AAACAAATAGGCACAGGATC+AGG + tig0023248:44340-44359 Msa1406980:intron 40.0%
! ACTACTAGCTTATGCATGTG+AGG + tig0023248:45168-45187 Msa1406980:intron 40.0%
! GGTTAGGTTCCTTTGTCTTT+TGG + tig0023248:45212-45231 Msa1406980:intron 40.0%
!! CTTTTTGTGATTTGCCTTGC+TGG + tig0023248:45297-45316 Msa1406980:intron 40.0%
! TGGTGATGTGAATTAGCTAC+TGG + tig0023248:45723-45742 Msa1406980:intron 40.0%
GATGTGAATTAGCTACTGGA+TGG + tig0023248:45727-45746 Msa1406980:intron 40.0%
GGGTCTAATATGATACTGTG+CGG + tig0023248:45943-45962 Msa1406980:intron 40.0%
GACCCACTCATTACATATTC+TGG + tig0023248:46519-46538 Msa1406980:intron 40.0%
GAGGACCTCATTTACTGAAT+TGG - tig0023248:46667-46686 None:intergenic 40.0%
GGCAACACTAAAATCCTTCA+TGG - tig0023248:46804-46823 None:intergenic 40.0%
! GTTGCCTTCAAGTTACCTTA+TGG + tig0023248:46818-46837 Msa1406980:intron 40.0%
CAATGTCAATTTCGGCCATA+AGG - tig0023248:46836-46855 None:intergenic 40.0%
AGCCGTGACACAATTCAAAT+AGG - tig0023248:47091-47110 None:intergenic 40.0%
TTGAATTGTGTCACGGCTTT+AGG + tig0023248:47093-47112 Msa1406980:intron 40.0%
AGTCATACGATGCATACATG+TGG + tig0023248:47192-47211 Msa1406980:intron 40.0%
GTCATACGATGCATACATGT+GGG + tig0023248:47193-47212 Msa1406980:intron 40.0%
TCATACGATGCATACATGTG+GGG + tig0023248:47194-47213 Msa1406980:intron 40.0%
TACATGTGGGGTTATTTGTG+TGG + tig0023248:47206-47225 Msa1406980:intron 40.0%
TCCGCGATTTATACTGGAAA+AGG + tig0023248:47233-47252 Msa1406980:intron 40.0%
! CCCTTTTCCAGTATAAATCG+CGG - tig0023248:47237-47256 None:intergenic 40.0%
CCGCGATTTATACTGGAAAA+GGG + tig0023248:47234-47253 Msa1406980:intron 40.0%
GAGTTACTCTTGTGTAGGAC+AGG - tig0023248:44469-44488 None:intergenic 45.0%
! AAGTATGTCTGAGATGCCCT+TGG + tig0023248:45820-45839 Msa1406980:intron 45.0%
TCGGCCATAAGGTAACTTGA+AGG - tig0023248:46825-46844 None:intergenic 45.0%
!! TTGTGTCACGGCTTTAGGAT+TGG + tig0023248:47098-47117 Msa1406980:intron 45.0%
ATGTGGGGTTATTTGTGTGG+AGG + tig0023248:47209-47228 Msa1406980:intron 45.0%
!!! TAATTTTCTTATTTTAATTT+TGG + tig0023248:45773-45792 Msa1406980:intron 5.0%
CATCGTTCTTCACCTGCACT+TGG - tig0023248:44223-44242 None:intergenic 50.0%
! ACAAGTGCTGCCTTTGTTGC+AGG + tig0023248:45129-45148 Msa1406980:intron 50.0%
TAGCTACTGGATGGTCACAC+TGG + tig0023248:45736-45755 Msa1406980:intron 50.0%
!! GGCTTTAGGATTGGAGACTC+TGG + tig0023248:47107-47126 Msa1406980:intron 50.0%
GGAGGCTCCGCGATTTATAC+TGG + tig0023248:47227-47246 Msa1406980:intron 55.0%
Chromosome Type Strat End Strand Name
tig0023248 gene 44012 47749 44012 ID=Msa1406980;Name=Msa1406980
tig0023248 mRNA 44012 47749 44012 ID=Msa1406980-mRNA-1;Parent=Msa1406980;Name=Msa1406980-mRNA-1;_AED=0.50;_eAED=0.61;_QI=190|0.66|0.75|1|0|0|4|174|25
tig0023248 exon 44012 44142 44012 ID=Msa1406980-mRNA-1:exon:24837;Parent=Msa1406980-mRNA-1
tig0023248 exon 44230 44324 44230 ID=Msa1406980-mRNA-1:exon:24838;Parent=Msa1406980-mRNA-1
tig0023248 exon 45010 45038 45010 ID=Msa1406980-mRNA-1:exon:24839;Parent=Msa1406980-mRNA-1
tig0023248 exon 47563 47749 47563 ID=Msa1406980-mRNA-1:exon:24840;Parent=Msa1406980-mRNA-1
tig0023248 five_prime_UTR 44012 44142 44012 ID=Msa1406980-mRNA-1:five_prime_utr;Parent=Msa1406980-mRNA-1
tig0023248 five_prime_UTR 44230 44288 44230 ID=Msa1406980-mRNA-1:five_prime_utr;Parent=Msa1406980-mRNA-1
tig0023248 CDS 44289 44324 44289 ID=Msa1406980-mRNA-1:cds;Parent=Msa1406980-mRNA-1
tig0023248 CDS 45010 45038 45010 ID=Msa1406980-mRNA-1:cds;Parent=Msa1406980-mRNA-1
tig0023248 CDS 47563 47575 47563 ID=Msa1406980-mRNA-1:cds;Parent=Msa1406980-mRNA-1
tig0023248 three_prime_UTR 47576 47749 47576 ID=Msa1406980-mRNA-1:three_prime_utr;Parent=Msa1406980-mRNA-1
Gene Sequence

>Msa1406980

ATGGTTCAAAGTATGTTGATGATGAGTGCTGAAAAGGTTCAATTCTTTTCAGTTGTGATGGAAAGGACTCATTTTTGA

Protein sequence

>Msa1406980

MVQSMLMMSAEKVQFFSVVMERTHF*