Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1417360 | sp|Q9ST43|PH1_ARATH | 70.833 | 144 | 42 | 0 | 1 | 144 | 1 | 144 | 2.72e-72 | 216 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1417360 | I3SI15 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 5.74e-105 | 305 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0101810 | Msa1417360 | 0.848206 | 7.102479e-60 | -8.615850e-47 |
| Msa1408620 | Msa1417360 | 0.800129 | 1.665610e-48 | -8.615850e-47 |
| Msa0679290 | Msa1417360 | 0.816220 | 6.186997e-52 | -8.615850e-47 |
| Msa0802470 | Msa1417360 | 0.802981 | 4.334963e-49 | -8.615850e-47 |
| Msa1307210 | Msa1417360 | -0.815320 | 9.821086e-52 | -8.615850e-47 |
| Msa1035310 | Msa1417360 | -0.801140 | 1.035959e-48 | -8.615850e-47 |
| Msa1116440 | Msa1417360 | -0.811854 | 5.685728e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1417360 | MtrunA17_Chr3g0104531 | 98.649 | 148 | 2 | 0 | 1 | 148 | 1 | 148 | 1.11e-108 | 305 |
| Msa1417360 | MtrunA17_Chr5g0436951 | 73.050 | 141 | 36 | 1 | 3 | 143 | 4 | 142 | 2.36e-75 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1417360 | AT2G29700.1 | 70.833 | 144 | 42 | 0 | 1 | 144 | 1 | 144 | 2.77e-73 | 216 |
| Msa1417360 | AT5G05710.1 | 65.517 | 145 | 47 | 2 | 1 | 144 | 1 | 143 | 3.06e-67 | 201 |
Find 44 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCGAAAGCCGACGCTTTA+TGG | 0.114579 | tig0024470:-36225 | None:intergenic |
| AGTCTGATGGGTTTGGTTCT+TGG | 0.195174 | tig0024470:-35985 | None:intergenic |
| ATTGGGAGAGCTATTGTTCT+TGG | 0.247287 | tig0024470:+36319 | Msa1417360:CDS |
| TTAAACAAGGCAAACTCTTC+TGG | 0.276166 | tig0024470:+36104 | Msa1417360:CDS |
| GTTAACCAACCGGAACGTTC+TGG | 0.303194 | tig0024470:-36034 | None:intergenic |
| GAGGATTGGATTAATTCTAT+TGG | 0.322590 | tig0024470:+36301 | Msa1417360:CDS |
| AGGATTGGATTAATTCTATT+GGG | 0.345661 | tig0024470:+36302 | Msa1417360:CDS |
| GAGAGTAGATGGAGAATCTA+TGG | 0.353854 | tig0024470:+35948 | None:intergenic |
| TTGGGAGAGCTATTGTTCTT+GGG | 0.379023 | tig0024470:+36320 | Msa1417360:CDS |
| GTTCTGGGTTTGACCAGAAT+TGG | 0.381583 | tig0024470:-36018 | None:intergenic |
| GTTTGACCAGAATTGGATTC+CGG | 0.397052 | tig0024470:-36011 | None:intergenic |
| ACTACACCGGAATCCAATTC+TGG | 0.417350 | tig0024470:+36005 | Msa1417360:CDS |
| TTAACCAACCGGAACGTTCT+GGG | 0.426572 | tig0024470:-36033 | None:intergenic |
| CCGGTGTAGTCTGATGGGTT+TGG | 0.429077 | tig0024470:-35992 | None:intergenic |
| TGGTCAAACCCAGAACGTTC+CGG | 0.429438 | tig0024470:+36025 | Msa1417360:CDS |
| GGGGACGGAGGAAGGAGAAG+TGG | 0.446111 | tig0024470:-36146 | None:intergenic |
| TGGATTCCGGTGTAGTCTGA+TGG | 0.463765 | tig0024470:-35998 | None:intergenic |
| AATCTATGGCGATCAGCAAC+AGG | 0.469096 | tig0024470:+35962 | Msa1417360:CDS |
| AGGCAAACTCTTCTGGTTCA+AGG | 0.475718 | tig0024470:+36111 | Msa1417360:CDS |
| TGCTGATAATGATAAGGATA+AGG | 0.488545 | tig0024470:+36279 | Msa1417360:CDS |
| TCCACTTGTTTAACTGTGAA+AGG | 0.493585 | tig0024470:+36187 | Msa1417360:CDS |
| ATGATAAGGATAAGGAGGAT+TGG | 0.493896 | tig0024470:+36287 | Msa1417360:CDS |
| AACGAACCAACGGCGACGCC+AGG | 0.506146 | tig0024470:-36080 | None:intergenic |
| CGTTGGTTCGTTCTTAAACA+AGG | 0.506367 | tig0024470:+36091 | Msa1417360:CDS |
| GAAGGAGAAGTGGCGGCGGA+TGG | 0.511240 | tig0024470:-36136 | None:intergenic |
| GACGGAGGAAGGAGAAGTGG+CGG | 0.516576 | tig0024470:-36143 | None:intergenic |
| TAGATCTCATTCTGCTTCTG+AGG | 0.523501 | tig0024470:+36345 | Msa1417360:CDS |
| GGATTCCGGTGTAGTCTGAT+GGG | 0.523944 | tig0024470:-35997 | None:intergenic |
| GGAGGAAGGAGAAGTGGCGG+CGG | 0.543117 | tig0024470:-36140 | None:intergenic |
| GGAGACGGAGATGACGCCAC+GGG | 0.559275 | tig0024470:-36167 | None:intergenic |
| TCAAAACCTGGCGTCGCCGT+TGG | 0.568058 | tig0024470:+36074 | Msa1417360:CDS |
| CACAGTTAAACAAGTGGAGA+CGG | 0.574952 | tig0024470:-36182 | None:intergenic |
| TCCGGTTGGTTAACAAAACA+AGG | 0.580273 | tig0024470:+36043 | Msa1417360:CDS |
| ACCTTTCACAGTTAAACAAG+TGG | 0.588114 | tig0024470:-36188 | None:intergenic |
| TGATAATGATAAGGATAAGG+AGG | 0.606730 | tig0024470:+36282 | Msa1417360:CDS |
| TTTAACTGTGAAAGGTGCTG+AGG | 0.638348 | tig0024470:+36195 | Msa1417360:CDS |
| GGATGTTCTCCATAAAGCGT+CGG | 0.643354 | tig0024470:+36216 | Msa1417360:CDS |
| CAAACCCAGAACGTTCCGGT+TGG | 0.645172 | tig0024470:+36029 | Msa1417360:CDS |
| CTTGTTTAAGAACGAACCAA+CGG | 0.646253 | tig0024470:-36090 | None:intergenic |
| AAGGTGACTACATCAAAACC+TGG | 0.648621 | tig0024470:+36062 | Msa1417360:CDS |
| TGGAGACGGAGATGACGCCA+CGG | 0.660219 | tig0024470:-36168 | None:intergenic |
| CCAAACCCATCAGACTACAC+CGG | 0.679550 | tig0024470:+35992 | Msa1417360:CDS |
| GAGACGGAGATGACGCCACG+GGG | 0.709720 | tig0024470:-36166 | None:intergenic |
| AGACGGAGATGACGCCACGG+GGG | 0.781483 | tig0024470:-36165 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATCAGCAATAAAAAACA+TGG | - | tig0024470:36269-36288 | None:intergenic | 20.0% |
| !!! | TTTTATTGCTGATAATGATA+AGG | + | tig0024470:36273-36292 | Msa1417360:CDS | 20.0% |
| ! | AGGATTGGATTAATTCTATT+GGG | + | tig0024470:36302-36321 | Msa1417360:CDS | 25.0% |
| ! | TGCTGATAATGATAAGGATA+AGG | + | tig0024470:36279-36298 | Msa1417360:CDS | 30.0% |
| ! | TGATAATGATAAGGATAAGG+AGG | + | tig0024470:36282-36301 | Msa1417360:CDS | 30.0% |
| GAGGATTGGATTAATTCTAT+TGG | + | tig0024470:36301-36320 | Msa1417360:CDS | 30.0% | |
| !!! | ACCTTGTTTTGTTAACCAAC+CGG | - | tig0024470:36047-36066 | None:intergenic | 35.0% |
| CTTGTTTAAGAACGAACCAA+CGG | - | tig0024470:36093-36112 | None:intergenic | 35.0% | |
| TTAAACAAGGCAAACTCTTC+TGG | + | tig0024470:36104-36123 | Msa1417360:CDS | 35.0% | |
| TCCACTTGTTTAACTGTGAA+AGG | + | tig0024470:36187-36206 | Msa1417360:CDS | 35.0% | |
| ACCTTTCACAGTTAAACAAG+TGG | - | tig0024470:36191-36210 | None:intergenic | 35.0% | |
| ATAAAAAACATGGTCGTCTG+CGG | - | tig0024470:36259-36278 | None:intergenic | 35.0% | |
| ATGATAAGGATAAGGAGGAT+TGG | + | tig0024470:36287-36306 | Msa1417360:CDS | 35.0% | |
| GTTTGACCAGAATTGGATTC+CGG | - | tig0024470:36014-36033 | None:intergenic | 40.0% | |
| ! | TCCGGTTGGTTAACAAAACA+AGG | + | tig0024470:36043-36062 | Msa1417360:CDS | 40.0% |
| AAGGTGACTACATCAAAACC+TGG | + | tig0024470:36062-36081 | Msa1417360:CDS | 40.0% | |
| !! | CGTTGGTTCGTTCTTAAACA+AGG | + | tig0024470:36091-36110 | Msa1417360:CDS | 40.0% |
| CACAGTTAAACAAGTGGAGA+CGG | - | tig0024470:36185-36204 | None:intergenic | 40.0% | |
| !! | TTTAACTGTGAAAGGTGCTG+AGG | + | tig0024470:36195-36214 | Msa1417360:CDS | 40.0% |
| ATTGGGAGAGCTATTGTTCT+TGG | + | tig0024470:36319-36338 | Msa1417360:CDS | 40.0% | |
| TTGGGAGAGCTATTGTTCTT+GGG | + | tig0024470:36320-36339 | Msa1417360:CDS | 40.0% | |
| TAGATCTCATTCTGCTTCTG+AGG | + | tig0024470:36345-36364 | Msa1417360:CDS | 40.0% | |
| AATCTATGGCGATCAGCAAC+AGG | + | tig0024470:35962-35981 | Msa1417360:CDS | 45.0% | |
| !! | AGTCTGATGGGTTTGGTTCT+TGG | - | tig0024470:35988-36007 | None:intergenic | 45.0% |
| ACTACACCGGAATCCAATTC+TGG | + | tig0024470:36005-36024 | Msa1417360:CDS | 45.0% | |
| ! | GTTCTGGGTTTGACCAGAAT+TGG | - | tig0024470:36021-36040 | None:intergenic | 45.0% |
| TTAACCAACCGGAACGTTCT+GGG | - | tig0024470:36036-36055 | None:intergenic | 45.0% | |
| !! | AGGCAAACTCTTCTGGTTCA+AGG | + | tig0024470:36111-36130 | Msa1417360:CDS | 45.0% |
| GGATGTTCTCCATAAAGCGT+CGG | + | tig0024470:36216-36235 | Msa1417360:CDS | 45.0% | |
| CCAAACCCATCAGACTACAC+CGG | + | tig0024470:35992-36011 | Msa1417360:CDS | 50.0% | |
| GGATTCCGGTGTAGTCTGAT+GGG | - | tig0024470:36000-36019 | None:intergenic | 50.0% | |
| TGGATTCCGGTGTAGTCTGA+TGG | - | tig0024470:36001-36020 | None:intergenic | 50.0% | |
| TGGTCAAACCCAGAACGTTC+CGG | + | tig0024470:36025-36044 | Msa1417360:CDS | 50.0% | |
| GTTAACCAACCGGAACGTTC+TGG | - | tig0024470:36037-36056 | None:intergenic | 50.0% | |
| !! | CCGGTGTAGTCTGATGGGTT+TGG | - | tig0024470:35995-36014 | None:intergenic | 55.0% |
| CAAACCCAGAACGTTCCGGT+TGG | + | tig0024470:36029-36048 | Msa1417360:CDS | 55.0% | |
| GCTCGAAAGCCGACGCTTTA+TGG | - | tig0024470:36228-36247 | None:intergenic | 55.0% | |
| TCAAAACCTGGCGTCGCCGT+TGG | + | tig0024470:36074-36093 | Msa1417360:CDS | 60.0% | |
| GACGGAGGAAGGAGAAGTGG+CGG | - | tig0024470:36146-36165 | None:intergenic | 60.0% | |
| TGGAGACGGAGATGACGCCA+CGG | - | tig0024470:36171-36190 | None:intergenic | 60.0% | |
| AACGAACCAACGGCGACGCC+AGG | - | tig0024470:36083-36102 | None:intergenic | 65.0% | |
| GAAGGAGAAGTGGCGGCGGA+TGG | - | tig0024470:36139-36158 | None:intergenic | 65.0% | |
| GGAGGAAGGAGAAGTGGCGG+CGG | - | tig0024470:36143-36162 | None:intergenic | 65.0% | |
| GGGGACGGAGGAAGGAGAAG+TGG | - | tig0024470:36149-36168 | None:intergenic | 65.0% | |
| AGACGGAGATGACGCCACGG+GGG | - | tig0024470:36168-36187 | None:intergenic | 65.0% | |
| GAGACGGAGATGACGCCACG+GGG | - | tig0024470:36169-36188 | None:intergenic | 65.0% | |
| GGAGACGGAGATGACGCCAC+GGG | - | tig0024470:36170-36189 | None:intergenic | 65.0% | |
| TCTCCTTCCTCCGTCCCCCG+TGG | + | tig0024470:36151-36170 | Msa1417360:CDS | 70.0% | |
| GGAGATGACGCCACGGGGGA+CGG | - | tig0024470:36164-36183 | None:intergenic | 70.0% | |
| ACGCCACGGGGGACGGAGGA+AGG | - | tig0024470:36157-36176 | None:intergenic | 75.0% | |
| GATGACGCCACGGGGGACGG+AGG | - | tig0024470:36161-36180 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0024470 | gene | 35956 | 36402 | 35956 | ID=Msa1417360;Name=Msa1417360 |
| tig0024470 | mRNA | 35956 | 36402 | 35956 | ID=Msa1417360-mRNA-1;Parent=Msa1417360;Name=Msa1417360-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|148 |
| tig0024470 | exon | 35956 | 36402 | 35956 | ID=Msa1417360-mRNA-1:exon:23351;Parent=Msa1417360-mRNA-1 |
| tig0024470 | CDS | 35956 | 36402 | 35956 | ID=Msa1417360-mRNA-1:cds;Parent=Msa1417360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
