AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1307210


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1307210 MtrunA17_Chr8g0346161 94.348 230 11 1 1 228 235 464 1.47e-157 444
Msa1307210 MtrunA17_Chr8g0346151 83.700 227 32 4 5 226 228 454 5.51e-129 371
Msa1307210 MtrunA17_Chr5g0411341 51.485 202 87 4 36 227 474 674 6.32e-59 196
Msa1307210 MtrunA17_Chr8g0366751 48.515 202 91 4 37 227 444 643 3.30e-55 186
Msa1307210 MtrunA17_Chr7g0260841 40.462 173 78 3 35 207 335 482 4.02e-33 124
Msa1307210 MtrunA17_Chr7g0275821 36.842 171 98 2 49 218 246 407 6.85e-29 112
Msa1307210 MtrunA17_Chr4g0054561 30.500 200 111 7 9 192 102 289 5.68e-19 84.0
Msa1307210 MtrunA17_Chr1g0185621 33.526 173 103 3 47 207 462 634 1.02e-18 84.3
Msa1307210 MtrunA17_Chr4g0002781 27.230 213 106 6 9 192 121 313 5.92e-18 81.3
Msa1307210 MtrunA17_Chr2g0333641 30.625 160 102 5 38 192 116 271 5.68e-17 78.2
Msa1307210 MtrunA17_Chr4g0002801 30.435 161 95 5 41 192 142 294 5.86e-17 78.2
Msa1307210 MtrunA17_Chr7g0225901 26.702 191 124 4 22 205 56 237 9.22e-17 76.6
Msa1307210 MtrunA17_Chr2g0333621 27.329 161 109 4 38 192 117 275 1.32e-16 77.0
Msa1307210 MtrunA17_Chr5g0400671 33.571 140 83 3 56 192 155 287 1.53e-16 77.0
Msa1307210 MtrunA17_Chr2g0307131 28.402 169 116 2 24 192 152 315 3.22e-16 76.6
Msa1307210 MtrunA17_Chr2g0287761 26.136 176 116 4 54 217 1 174 5.67e-16 73.6
Msa1307210 MtrunA17_Chr1g0211081 28.070 228 121 6 7 194 259 483 8.73e-16 75.9
Msa1307210 MtrunA17_Chr6g0468491 33.333 144 87 4 26 167 112 248 1.13e-15 74.7
Msa1307210 MtrunA17_Chr4g0049331 28.261 184 112 5 29 205 114 284 1.15e-15 74.3
Msa1307210 MtrunA17_Chr2g0307141 30.496 141 91 2 55 192 183 319 1.41e-15 74.7
Msa1307210 MtrunA17_Chr4g0033291 31.655 139 79 3 28 165 138 261 1.74e-15 74.3
Msa1307210 MtrunA17_Chr4g0033391 27.011 174 106 5 25 192 122 280 2.74e-15 73.6
Msa1307210 MtrunA17_Chr4g0054551 28.643 199 115 7 9 194 102 286 3.34e-15 73.2
Msa1307210 MtrunA17_Chr8g0386921 38.835 103 53 2 54 146 405 507 1.97e-14 72.0
Msa1307210 MtrunA17_Chr7g0252041 60.000 55 22 0 53 107 284 338 1.99e-14 71.6
Msa1307210 MtrunA17_Chr4g0033281 26.214 206 130 6 29 228 137 326 2.30e-14 70.9
Msa1307210 MtrunA17_Chr2g0280161 28.931 159 97 3 55 205 177 327 2.36e-14 71.2
Msa1307210 MtrunA17_Chr3g0131691 58.182 55 23 0 53 107 296 350 2.57e-14 71.2
Msa1307210 MtrunA17_Chr5g0400661 28.415 183 110 7 17 184 111 287 3.50e-14 70.5
Msa1307210 MtrunA17_Chr4g0054571 34.058 138 78 4 40 167 134 268 4.54e-14 70.1
Msa1307210 MtrunA17_Chr6g0468461 37.838 111 61 2 26 136 148 250 4.69e-14 70.1
Msa1307210 MtrunA17_Chr2g0298751 53.226 62 22 1 56 110 53 114 1.11e-13 68.2
Msa1307210 MtrunA17_Chr4g0054581 49.254 67 30 1 55 121 198 260 2.38e-13 68.6
Msa1307210 MtrunA17_Chr8g0336681 25.316 158 112 2 41 192 142 299 3.18e-12 65.1
Msa1307210 MtrunA17_Chr4g0052841 29.448 163 82 6 56 197 293 443 7.64e-12 64.3
Msa1307210 MtrunA17_Chr4g0028071 30.488 164 71 8 59 200 153 295 1.18e-11 63.2
Msa1307210 MtrunA17_Chr2g0320291 29.375 160 88 8 59 207 137 282 1.53e-11 62.8
Msa1307210 MtrunA17_Chr8g0365711 28.289 152 85 4 59 196 248 389 2.52e-11 62.4
Msa1307210 MtrunA17_Chr5g0400641 29.221 154 87 6 59 204 2 141 4.97e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1307210 AT1G32640.1 54.082 196 82 4 35 227 433 623 7.51e-60 198
Msa1307210 AT4G17880.1 48.223 197 92 3 36 228 397 587 1.53e-57 192
Msa1307210 AT5G46760.1 48.241 199 93 4 36 228 396 590 1.46e-56 189
Msa1307210 AT5G46830.1 50.575 174 79 4 46 217 334 502 3.64e-44 155
Msa1307210 AT4G00870.1 51.397 179 76 4 54 226 248 421 1.02e-41 147
Msa1307210 AT2G46510.1 41.398 186 87 4 35 218 376 541 4.54e-36 134
Msa1307210 AT4G16430.1 38.144 194 94 4 35 228 300 467 6.33e-33 124
Msa1307210 AT1G01260.1 37.561 205 101 4 14 218 394 571 7.89e-33 125
Msa1307210 AT1G01260.2 37.561 205 101 4 14 218 394 571 7.89e-33 125
Msa1307210 AT1G01260.3 37.561 205 101 4 14 218 394 571 7.89e-33 125
Msa1307210 AT4G37850.2 31.325 166 104 5 32 192 130 290 1.15e-16 77.8
Msa1307210 AT4G37850.1 31.325 166 104 5 32 192 130 290 1.15e-16 77.8
Msa1307210 AT1G63650.3 27.711 166 103 3 55 203 405 570 2.06e-16 78.2
Msa1307210 AT1G63650.1 27.711 166 103 3 55 203 405 570 2.06e-16 78.2
Msa1307210 AT1G63650.2 27.711 166 103 3 55 203 405 570 2.06e-16 78.2
Msa1307210 AT4G09820.1 33.588 131 64 2 54 183 362 470 1.26e-15 75.9
Msa1307210 AT3G26744.1 60.000 55 22 0 53 107 305 359 4.55e-15 73.9
Msa1307210 AT3G26744.4 60.000 55 22 0 53 107 305 359 4.55e-15 73.9
Msa1307210 AT3G26744.2 60.000 55 22 0 53 107 305 359 4.55e-15 73.9
Msa1307210 AT3G26744.6 60.000 55 22 0 53 107 305 359 4.62e-15 73.9
Msa1307210 AT1G12860.2 57.895 57 24 0 53 109 265 321 4.87e-15 73.9
Msa1307210 AT1G12860.1 57.895 57 24 0 53 109 265 321 5.33e-15 73.9
Msa1307210 AT3G06120.1 27.869 183 111 4 54 215 1 183 1.05e-14 70.5
Msa1307210 AT5G57150.1 26.721 247 137 10 1 227 9 231 1.51e-14 70.9
Msa1307210 AT5G57150.2 27.315 216 116 8 13 207 20 215 2.33e-14 70.1
Msa1307210 AT5G57150.3 26.852 216 117 8 13 207 20 215 3.52e-14 69.3
Msa1307210 AT5G57150.5 37.719 114 50 4 13 125 20 113 3.68e-14 69.3
Msa1307210 AT5G57150.4 27.397 219 117 9 13 209 20 218 4.16e-14 70.1
Msa1307210 AT4G29930.5 54.098 61 21 1 56 109 55 115 6.15e-14 68.9
Msa1307210 AT4G29930.2 45.238 84 34 2 33 109 37 115 6.77e-14 68.9
Msa1307210 AT4G29930.6 54.098 61 21 1 56 109 55 115 7.74e-14 68.6
Msa1307210 AT4G29930.3 54.098 61 21 1 56 109 55 115 1.17e-13 68.6
Msa1307210 AT4G29930.7 54.098 61 21 1 56 109 55 115 1.17e-13 68.6
Msa1307210 AT4G29930.1 54.098 61 21 1 56 109 55 115 1.33e-13 68.6
Msa1307210 AT4G29930.4 55.000 60 20 1 57 109 56 115 1.58e-13 67.0
Msa1307210 AT1G10610.2 32.847 137 85 4 56 187 235 369 6.52e-13 67.4
Msa1307210 AT1G10610.1 32.847 137 85 4 56 187 265 399 6.60e-13 67.8
Msa1307210 AT1G10610.3 32.847 137 85 4 56 187 168 302 7.36e-13 67.0
Msa1307210 AT5G41315.3 32.258 93 49 1 55 147 446 524 8.08e-13 67.4
Msa1307210 AT5G41315.2 32.258 93 49 1 55 147 449 527 8.40e-13 67.4
Msa1307210 AT5G41315.1 32.258 93 49 1 55 147 441 519 8.61e-13 67.4
Msa1307210 AT2G22760.2 28.415 183 106 7 42 205 100 276 1.80e-12 65.5
Msa1307210 AT2G16910.1 54.000 50 23 0 59 108 318 367 2.38e-12 66.2
Msa1307210 AT2G22760.1 29.697 165 96 6 42 192 100 258 3.51e-12 64.7
Msa1307210 AT3G24140.2 24.352 193 122 3 54 222 197 389 1.12e-11 63.9
Msa1307210 AT3G24140.1 24.352 193 122 3 54 222 197 389 1.21e-11 63.9
Msa1307210 AT4G21330.1 30.065 153 85 3 56 196 33 175 2.29e-11 61.6
Msa1307210 AT4G38070.1 38.095 84 49 1 24 107 67 147 3.98e-11 61.6
Msa1307210 AT2G22750.2 27.465 142 99 1 55 192 126 267 5.33e-11 61.6
Msa1307210 AT2G22750.1 27.465 142 99 1 55 192 126 267 5.41e-11 61.6
Msa1307210 AT2G28160.2 50.980 51 25 0 59 109 135 185 5.99e-11 61.6
Msa1307210 AT2G28160.1 50.980 51 25 0 59 109 135 185 6.62e-11 61.2
Msa1307210 AT2G22750.5 27.465 142 99 1 55 192 150 291 8.14e-11 61.2
Msa1307210 AT2G22750.4 27.465 142 99 1 55 192 154 295 8.15e-11 61.2
Msa1307210 AT2G22750.3 27.465 142 99 1 55 192 154 295 8.74e-11 61.2

Find 32 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGAAGGATTTGGAGTTTC+AGG 0.119847 8_4:-2606991 Msa1307210:CDS
GAGATTGATGTGAAGATAAT+AGG 0.216324 8_4:-2607080 Msa1307210:CDS
TTATCCATCTTTGAAACATT+TGG 0.290544 8_4:+2607293 None:intergenic
ATCTTAAAGCATAGAATCTA+TGG 0.304209 8_4:+2607324 None:intergenic
ATTCTTGTTGGTGTTGCTAA+TGG 0.335278 8_4:+2607464 None:intergenic
AATGAGTGTCTTGAAGGATT+TGG 0.342944 8_4:-2607000 Msa1307210:CDS
AAGATTGAAGACTTAGAATC+AGG 0.369405 8_4:-2607221 Msa1307210:CDS
AGAGGAAGAAAACCGTTATC+AGG 0.381422 8_4:-2607404 Msa1307210:CDS
TCAGCCATTCTCATGAAATT+AGG 0.386390 8_4:-2606870 Msa1307210:CDS
ATCGACTGTTAATGAAGTTA+TGG 0.398979 8_4:-2606952 Msa1307210:CDS
AAGATTAATGAGTGTCTTGA+AGG 0.413301 8_4:-2607006 Msa1307210:CDS
TCTGCTTCCACATGGTTTAA+CGG 0.415651 8_4:+2607371 None:intergenic
GGTTCCAAATGTTTCAAAGA+TGG 0.435449 8_4:-2607297 Msa1307210:CDS
AAAAGTGAAGATGGAAATGA+TGG 0.445716 8_4:-2607180 Msa1307210:CDS
TCTTCCGTGCTCATCTCGTT+AGG 0.461472 8_4:+2606906 None:intergenic
CTGACCTAATTTCATGAGAA+TGG 0.482002 8_4:+2606866 None:intergenic
CTTAGAATCAGGGCAACAAA+AGG 0.502345 8_4:-2607210 Msa1307210:CDS
GGTGTTTGAGTTCCTGATAA+CGG 0.508977 8_4:+2607392 None:intergenic
TTTGTCTCTCTGCTTCCACA+TGG 0.518910 8_4:+2607363 None:intergenic
ATTCTATGCTTTAAGATCTG+TGG 0.527981 8_4:-2607318 Msa1307210:CDS
ACACTCATTAATCTTGCACT+AGG 0.531898 8_4:+2607014 None:intergenic
ATGTGGAAGCAGAGAGACAA+AGG 0.547229 8_4:-2607361 Msa1307210:CDS
TCAAAAGCATTAACATTACA+AGG 0.560113 8_4:+2607101 None:intergenic
CTTGTTGGTGTTGCTAATGG+TGG 0.567083 8_4:+2607467 None:intergenic
GATAATAGGAAATGATGCTA+TGG 0.577854 8_4:-2607066 Msa1307210:CDS
TGCAACGGCAACTTCAACTG+TGG 0.578390 8_4:-2607138 Msa1307210:CDS
AGATTGAAGACTTAGAATCA+GGG 0.588379 8_4:-2607220 Msa1307210:CDS
AAAAGAGAACCTAAGAAGAG+AGG 0.611347 8_4:-2607422 Msa1307210:CDS
TGTCGATAATAACAGTGCAA+CGG 0.631393 8_4:-2607153 Msa1307210:CDS
CACTCATTAATCTTGCACTA+GGG 0.642950 8_4:+2607015 None:intergenic
AGTTCCTAACGAGATGAGCA+CGG 0.664680 8_4:-2606910 Msa1307210:CDS
TCAAACACCGTTAAACCATG+TGG 0.685029 8_4:-2607378 Msa1307210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATCTTTTTCAATTCTTGT+TGG + chr8_4:2606941-2606960 None:intergenic 25.0%
! ATCTTAAAGCATAGAATCTA+TGG + chr8_4:2607069-2607088 None:intergenic 25.0%
!!! TTATCCATCTTTGAAACATT+TGG + chr8_4:2607100-2607119 None:intergenic 25.0%
!!! TAACATTACAAGGTCTTTTT+TGG + chr8_4:2607282-2607301 None:intergenic 25.0%
!!! TCAAAAGCATTAACATTACA+AGG + chr8_4:2607292-2607311 None:intergenic 25.0%
!! ATTCTATGCTTTAAGATCTG+TGG - chr8_4:2607072-2607091 Msa1307210:CDS 30.0%
AAGATTGAAGACTTAGAATC+AGG - chr8_4:2607169-2607188 Msa1307210:CDS 30.0%
AGATTGAAGACTTAGAATCA+GGG - chr8_4:2607170-2607189 Msa1307210:CDS 30.0%
AAAAGTGAAGATGGAAATGA+TGG - chr8_4:2607210-2607229 Msa1307210:CDS 30.0%
GAGATTGATGTGAAGATAAT+AGG - chr8_4:2607310-2607329 Msa1307210:CDS 30.0%
GATAATAGGAAATGATGCTA+TGG - chr8_4:2607324-2607343 Msa1307210:CDS 30.0%
AAGATTAATGAGTGTCTTGA+AGG - chr8_4:2607384-2607403 Msa1307210:CDS 30.0%
ATCGACTGTTAATGAAGTTA+TGG - chr8_4:2607438-2607457 Msa1307210:CDS 30.0%
!! ATTCTTGTTGGTGTTGCTAA+TGG + chr8_4:2606929-2606948 None:intergenic 35.0%
AAAAGAGAACCTAAGAAGAG+AGG - chr8_4:2606968-2606987 Msa1307210:CDS 35.0%
GGTTCCAAATGTTTCAAAGA+TGG - chr8_4:2607093-2607112 Msa1307210:CDS 35.0%
! TTCTCTTTTATCAGATGCAG+TGG - chr8_4:2607123-2607142 Msa1307210:CDS 35.0%
GGAATCGAAAAAAGTGAAGA+TGG - chr8_4:2607201-2607220 Msa1307210:CDS 35.0%
TGTCGATAATAACAGTGCAA+CGG - chr8_4:2607237-2607256 Msa1307210:CDS 35.0%
CACTCATTAATCTTGCACTA+GGG + chr8_4:2607378-2607397 None:intergenic 35.0%
ACACTCATTAATCTTGCACT+AGG + chr8_4:2607379-2607398 None:intergenic 35.0%
AATGAGTGTCTTGAAGGATT+TGG - chr8_4:2607390-2607409 Msa1307210:CDS 35.0%
TCAGCCATTCTCATGAAATT+AGG - chr8_4:2607520-2607539 Msa1307210:CDS 35.0%
CTGACCTAATTTCATGAGAA+TGG + chr8_4:2607527-2607546 None:intergenic 35.0%
! GGTTTTCTTCCTCTCTTCTT+AGG + chr8_4:2606980-2606999 None:intergenic 40.0%
AGAGGAAGAAAACCGTTATC+AGG - chr8_4:2606986-2607005 Msa1307210:CDS 40.0%
! GGTGTTTGAGTTCCTGATAA+CGG + chr8_4:2607001-2607020 None:intergenic 40.0%
TCAAACACCGTTAAACCATG+TGG - chr8_4:2607012-2607031 Msa1307210:CDS 40.0%
TCTGCTTCCACATGGTTTAA+CGG + chr8_4:2607022-2607041 None:intergenic 40.0%
CTTAGAATCAGGGCAACAAA+AGG - chr8_4:2607180-2607199 Msa1307210:CDS 40.0%
! CTTGAAGGATTTGGAGTTTC+AGG - chr8_4:2607399-2607418 Msa1307210:CDS 40.0%
!! CTTGTTGGTGTTGCTAATGG+TGG + chr8_4:2606926-2606945 None:intergenic 45.0%
TTTGTCTCTCTGCTTCCACA+TGG + chr8_4:2607030-2607049 None:intergenic 45.0%
ATGTGGAAGCAGAGAGACAA+AGG - chr8_4:2607029-2607048 Msa1307210:CDS 45.0%
AGTTCCTAACGAGATGAGCA+CGG - chr8_4:2607480-2607499 Msa1307210:CDS 45.0%
TGCAACGGCAACTTCAACTG+TGG - chr8_4:2607252-2607271 Msa1307210:CDS 50.0%
TCTTCCGTGCTCATCTCGTT+AGG + chr8_4:2607487-2607506 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 2606863 2607549 2606863 ID=Msa1307210;Name=Msa1307210
chr8_4 mRNA 2606863 2607549 2606863 ID=Msa1307210-mRNA-1;Parent=Msa1307210;Name=Msa1307210-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|228
chr8_4 exon 2606863 2607549 2606863 ID=Msa1307210-mRNA-1:exon:802;Parent=Msa1307210-mRNA-1
chr8_4 CDS 2606863 2607549 2606863 ID=Msa1307210-mRNA-1:cds;Parent=Msa1307210-mRNA-1
Gene Sequence

>Msa1307210

ATGAATAACATTGTTTCATCATGTTCTTTTGATTCTGAAAATTCTGATTCAGAATATTGTCCACCATTAGCAACACCAACAAGAATTGAAAAAGATTCTTTTGAGAAAAGAGAACCTAAGAAGAGAGGAAGAAAACCGTTATCAGGAACTCAAACACCGTTAAACCATGTGGAAGCAGAGAGACAAAGGAGAGAGAAACTAAACCATAGATTCTATGCTTTAAGATCTGTGGTTCCAAATGTTTCAAAGATGGATAAAGCTTCTCTTTTATCAGATGCAGTGGATTACATCAACGAGTTAAAAGCAAAGATTGAAGACTTAGAATCAGGGCAACAAAAGGAATCGAAAAAAGTGAAGATGGAAATGATGGAAATTGTCGATAATAACAGTGCAACGGCAACTTCAACTGTGGTAGACCAAAAAAGACCTTGTAATGTTAATGCTTTTGAGATTGATGTGAAGATAATAGGAAATGATGCTATGGTGAGAGTTCAAAGTGAGAATGTTAATCACCCTAGTGCAAGATTAATGAGTGTCTTGAAGGATTTGGAGTTTCAGGTACATCATGCTAGTATATCGACTGTTAATGAAGTTATGGTTCAAGATGTTGTTGTTCGAGTTCCTAACGAGATGAGCACGGAAGAGAGTCTAATTAGATCAGCCATTCTCATGAAATTAGGTCAGTAG

Protein sequence

>Msa1307210

MNNIVSSCSFDSENSDSEYCPPLATPTRIEKDSFEKREPKKRGRKPLSGTQTPLNHVEAERQRREKLNHRFYALRSVVPNVSKMDKASLLSDAVDYINELKAKIEDLESGQQKESKKVKMEMMEIVDNNSATATSTVVDQKRPCNVNAFEIDVKIIGNDAMVRVQSENVNHPSARLMSVLKDLEFQVHHASISTVNEVMVQDVVVRVPNEMSTEESLIRSAILMKLGQ*