AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1420380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1420380 MtrunA17_Chr4g0059361 97.340 188 4 1 1 188 1 187 2.69e-134 373
Msa1420380 MtrunA17_Chr3g0117971 54.974 191 75 3 1 188 1 183 2.41e-63 193
Msa1420380 MtrunA17_Chr5g0414281 42.328 189 87 4 1 186 1 170 6.96e-40 133
Msa1420380 MtrunA17_Chr4g0054191 38.624 189 94 4 1 188 1 168 6.40e-35 120
Msa1420380 MtrunA17_Chr8g0364591 38.378 185 99 4 1 185 1 170 4.13e-30 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1420380 AT5G62900.1 46.809 188 73 4 1 188 1 161 9.32e-48 153
Msa1420380 AT5G50090.1 44.444 189 73 4 1 188 1 158 6.64e-42 139
Msa1420380 AT5G50090.2 46.277 188 65 5 1 188 1 152 2.77e-41 137
Msa1420380 AT1G60010.1 41.489 188 95 4 1 188 1 173 1.26e-40 135
Msa1420380 AT5G50090.3 52.500 120 43 1 1 120 1 106 1.13e-35 122
Msa1420380 AT1G10530.1 35.638 188 99 3 1 188 1 166 8.72e-35 120
Msa1420380 AT5G67620.1 32.195 205 98 7 1 188 1 181 1.06e-23 92.8

Find 142 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTAATCACTTTAAATT+TGG 0.165381 tig0024801:+75325 Msa1420380:three_prime_UTR
GAAGGAGAAGTAGTTGAAAA+AGG 0.168731 tig0024801:-75587 Msa1420390:CDS
TAAGGGCAAAATTCTTAATT+AGG 0.175331 tig0024801:-75368 None:intergenic
TGCATTTCATCCCTGTAATT+AGG 0.175438 tig0024801:+75248 Msa1420380:three_prime_UTR
ACAACACAATTCAATTTCTT+TGG 0.195753 tig0024801:+76207 Msa1420380:three_prime_UTR
GTGGCGTCTGTTTAATTTGC+TGG 0.207124 tig0024801:-76483 None:intergenic
ATCTGTGGGAAATTAAATTA+AGG 0.228969 tig0024801:+75217 Msa1420380:three_prime_UTR
GTTTCGTCTTCTCATGTTTC+CGG 0.232857 tig0024801:+75944 Msa1420380:three_prime_UTR
ACTTGTTGGGATTTAGAAAT+TGG 0.236727 tig0024801:+76054 Msa1420380:three_prime_UTR
GCATTTCATCCCTGTAATTA+GGG 0.241125 tig0024801:+75249 Msa1420380:three_prime_UTR
TTAAAGTGATTAATAATTAC+AGG 0.275595 tig0024801:-75338 None:intergenic
CCTCCTTCACTTCACTTCTC+TGG 0.278732 tig0024801:+75443 Msa1420380:three_prime_UTR
ACGTGTCTCAGATTTCATTA+AGG 0.285739 tig0024801:-75386 None:intergenic
GAGGAAGGATATAAATAATA+AGG 0.287758 tig0024801:-73962 None:intergenic
GACGATTTCGATTCTGATTC+CGG 0.302020 tig0024801:+75885 Msa1420380:three_prime_UTR
AACCTTTCTTCTTTGCCATT+TGG 0.302263 tig0024801:-74063 None:intergenic
ACAAAAGTCAAATCAGATTA+AGG 0.303462 tig0024801:+74512 Msa1420380:CDS
TTCCACCGCCGGAGGTTGTT+GGG 0.305016 tig0024801:-75842 Msa1420390:CDS
TTCAAATGACCCCTAATTAC+AGG 0.309823 tig0024801:-75278 None:intergenic
AATGACCCAAATGCAAAATA+TGG 0.311728 tig0024801:-76029 None:intergenic
ACAATACAAGGTTACTTTGA+TGG 0.315036 tig0024801:+76178 Msa1420380:three_prime_UTR
TAATAATTACAGGGATGAAA+TGG 0.325643 tig0024801:-75328 None:intergenic
CGTGTCTCAGATTTCATTAA+GGG 0.327473 tig0024801:-75385 None:intergenic
TTTCCACCGCCGGAGGTTGT+TGG 0.355496 tig0024801:-75843 Msa1420390:CDS
AGATTTACCTTGTTATCTTC+AGG 0.363890 tig0024801:-74570 None:intergenic
ACATTGGAAAATTCTAGTTC+AGG 0.365363 tig0024801:-75670 Msa1420390:intron
CGGCACGGTGGCGGCGGTGG+TGG 0.368020 tig0024801:+75964 Msa1420380:three_prime_UTR
TTAATGATAAAACAGCTAAT+AGG 0.376595 tig0024801:+74541 Msa1420380:CDS
GGTGGCGGCGGTGGTGGTGA+TGG 0.390206 tig0024801:+75970 Msa1420380:three_prime_UTR
AAAGAAGAAAAGAAGAAGAC+AGG 0.398304 tig0024801:+76416 Msa1420380:three_prime_UTR
TTCCGGCACGGTGGCGGCGG+TGG 0.401783 tig0024801:+75961 Msa1420380:three_prime_UTR
ATCACTTCACTAGCAGTAAC+TGG 0.406024 tig0024801:-74093 None:intergenic
ATTGTTTCTTGTGTCAAGTA+AGG 0.406562 tig0024801:+76030 Msa1420380:three_prime_UTR
CGGTCGGCGGTTTCCACCGC+CGG 0.413698 tig0024801:-75853 Msa1420390:CDS
ACAACAACAACGTCATCTTC+TGG 0.415357 tig0024801:+75679 Msa1420380:three_prime_UTR
CAATTCAATTTCTTTGGAAA+CGG 0.418868 tig0024801:+76213 Msa1420380:three_prime_UTR
AGAAGAGCAACATAGTGATC+AGG 0.427746 tig0024801:-74126 None:intergenic
TGGCGTCTGTTTAATTTGCT+GGG 0.430494 tig0024801:-76482 None:intergenic
AATAATTACAGGGATGAAAT+GGG 0.438337 tig0024801:-75327 None:intergenic
CATTGGAAAATTCTAGTTCA+GGG 0.442928 tig0024801:-75669 Msa1420390:intron
CAACAACAACGTCATCTTCT+GGG 0.443210 tig0024801:+75680 Msa1420380:three_prime_UTR
TCTGTGGGAAATTAAATTAA+GGG 0.443932 tig0024801:+75218 Msa1420380:three_prime_UTR
CTACAAGTAAGTGAAGGATC+AGG 0.455787 tig0024801:-75941 Msa1420390:CDS
CAGAGGTTACAAAGGGTATG+TGG 0.456771 tig0024801:+74442 Msa1420380:intron
AGGACGGGTAAGAAGAAAAG+AGG 0.459154 tig0024801:+76388 Msa1420380:three_prime_UTR
TCATGTTTCCGGCACGGTGG+CGG 0.467660 tig0024801:+75955 Msa1420380:three_prime_UTR
AGATGAACATAGGGGTAGAC+AGG 0.468639 tig0024801:-75426 None:intergenic
AGGAGACAACGGAGAGAGAA+TGG 0.469757 tig0024801:+76261 Msa1420380:three_prime_UTR
GAGACAACGGAGTGCGGCGG+AGG 0.469833 tig0024801:+76241 Msa1420380:three_prime_UTR
TTCAGTGATGCTTTGTAAAG+AGG 0.477664 tig0024801:-75147 None:intergenic
GCAAAGAAGAAAGGTTGTAT+TGG 0.478560 tig0024801:+74070 Msa1420380:CDS
TGTCAAGTAAGGAACTTGTT+GGG 0.479149 tig0024801:+76041 Msa1420380:three_prime_UTR
TCCCCAACAACCTCCGGCGG+TGG 0.479641 tig0024801:+75821 Msa1420380:three_prime_UTR
GTGTCAAGTAAGGAACTTGT+TGG 0.482790 tig0024801:+76040 Msa1420380:three_prime_UTR
CACCACCGCCGCCACCGTGC+CGG 0.484275 tig0024801:-75982 Msa1420390:CDS
ATGGGAGGATTAGTAAGCTC+AGG 0.484705 tig0024801:-75309 None:intergenic
GAAATCGTCGGAGTGAGCTT+AGG 0.485708 tig0024801:-75890 Msa1420390:CDS
GGACGGGTAAGAAGAAAAGA+GGG 0.487733 tig0024801:+76389 Msa1420380:three_prime_UTR
AGGACAAAAGCAACAACAAA+TGG 0.492369 tig0024801:+75070 Msa1420380:CDS
TGAAGACGAATCTGTGAAAG+AGG 0.500920 tig0024801:-76174 None:intergenic
AATTTCTTTGGAAACGGTGG+CGG 0.505295 tig0024801:+76219 Msa1420380:three_prime_UTR
TGTTTCCGGCACGGTGGCGG+CGG 0.505907 tig0024801:+75958 Msa1420380:three_prime_UTR
CGGGTAAGAAGAAAAGAGGG+AGG 0.510871 tig0024801:+76392 Msa1420380:three_prime_UTR
TTTGTGTCCCCAACAACCTC+CGG 0.512164 tig0024801:+75815 Msa1420380:three_prime_UTR
TCAGTGATGCTTTGTAAAGA+GGG 0.514695 tig0024801:-75146 None:intergenic
AAGAAGAAAAGAAGAAGACA+GGG 0.516920 tig0024801:+76417 Msa1420380:three_prime_UTR
GTTGCTATAAATATTCTAGT+AGG 0.517379 tig0024801:+73912 Msa1420380:five_prime_UTR
TCCACCGCCGGAGGTTGTTG+GGG 0.517578 tig0024801:-75841 Msa1420390:CDS
AACCAAATGGCAAAGAAGAA+AGG 0.522282 tig0024801:+74061 Msa1420380:CDS
GAAGAGTTCGATGGTGTTGG+TGG 0.522541 tig0024801:+76305 Msa1420380:three_prime_UTR
CCAGAGAAGTGAAGTGAAGG+AGG 0.526333 tig0024801:-75462 None:intergenic
TACTCACATGTGATGACTCA+AGG 0.527011 tig0024801:+75179 Msa1420380:three_prime_UTR
ATTGAAGAGTTCGATGGTGT+TGG 0.528757 tig0024801:+76302 Msa1420380:three_prime_UTR
TTTGACTCTTTGAAAACTAG+TGG 0.528818 tig0024801:+73875 Msa1420380:five_prime_UTR
GAGATCTGGTGTTGGTGTGG+TGG 0.531169 tig0024801:+76342 Msa1420380:three_prime_UTR
TTGGTTTCAGAGGTTACAAA+GGG 0.536135 tig0024801:+74435 Msa1420380:intron
TTCAATTTCTTTGGAAACGG+TGG 0.537158 tig0024801:+76216 Msa1420380:three_prime_UTR
TCAGGGAGGGAACTCAGAAC+CGG 0.537465 tig0024801:-75923 Msa1420390:CDS
GATTCAAAACTCATATCAGC+TGG 0.538326 tig0024801:-75174 None:intergenic
TTGATGAGGAAATCAATTGA+AGG 0.541033 tig0024801:-75605 Msa1420390:CDS
ACTAGTGGTAAGAGAGTGAG+TGG 0.541714 tig0024801:+73890 Msa1420380:five_prime_UTR
CTTGTGATACAGCAACCAAA+TGG 0.541988 tig0024801:+74048 Msa1420380:CDS
ACATGATTGCTTAGATCTGT+GGG 0.557047 tig0024801:+75203 Msa1420380:three_prime_UTR
GATTCGTCTTCAACAATACA+AGG 0.557195 tig0024801:+76166 Msa1420380:three_prime_UTR
GACATGATTGCTTAGATCTG+TGG 0.558047 tig0024801:+75202 Msa1420380:three_prime_UTR
TTTCTTCTTCAAGAAGAGGA+AGG 0.558868 tig0024801:-73977 None:intergenic
TGATAGACATGGAAGAGATC+TGG 0.559713 tig0024801:+76328 Msa1420380:three_prime_UTR
GTCTTCTCATGTTTCCGGCA+CGG 0.562757 tig0024801:+75949 Msa1420380:three_prime_UTR
GTGAGCTTAGGTAGACGGAA+AGG 0.568945 tig0024801:-75878 Msa1420390:CDS
TGAGCTTAGGTAGACGGAAA+GGG 0.574700 tig0024801:-75877 Msa1420390:CDS
GAAGAGCAACATAGTGATCA+GGG 0.575247 tig0024801:-74125 None:intergenic
CCAAGCAAAAGTGTATCTGT+TGG 0.576593 tig0024801:-74270 None:intergenic
TACAAGTAAGTGAAGGATCA+GGG 0.577021 tig0024801:-75940 Msa1420390:CDS
GTTCCAGAGAAGTGAAGTGA+AGG 0.577749 tig0024801:-75465 None:intergenic
CCAAATGCAAAATATGGCTA+CGG 0.579931 tig0024801:-76023 None:intergenic
TTTGGTTTCAGAGGTTACAA+AGG 0.584060 tig0024801:+74434 Msa1420380:intron
AGAGGTTACAAAGGGTATGT+GGG 0.585748 tig0024801:+74443 Msa1420380:intron
TCGGAGTGAGCTTAGGTAGA+CGG 0.588237 tig0024801:-75883 Msa1420390:CDS
AGACGGAAAGGGACGGTCGG+CGG 0.591139 tig0024801:-75866 Msa1420390:CDS
GAGTGCGGCGGAGGAGACAA+CGG 0.591412 tig0024801:+76250 Msa1420380:three_prime_UTR
GCGGAGACAACGGAGTGCGG+CGG 0.592465 tig0024801:+76238 Msa1420380:three_prime_UTR
TAAAGTGATTAATAATTACA+GGG 0.593348 tig0024801:-75337 None:intergenic
TATAGACTCATCTCAGCTCA+AGG 0.593800 tig0024801:+74300 Msa1420380:CDS
AAATAATAAGGAAGATGCAA+CGG 0.598797 tig0024801:-73950 None:intergenic
GGAGAGAGAATGGACGGTGT+TGG 0.601627 tig0024801:+76271 Msa1420380:three_prime_UTR
TCAAATGACCCCTAATTACA+GGG 0.604479 tig0024801:-75277 None:intergenic
TGGATCTATCCACAACCACA+AGG 0.605488 tig0024801:-75520 None:intergenic
GGGTAAGAAGAAAAGAGGGA+GGG 0.606847 tig0024801:+76393 Msa1420380:three_prime_UTR
GAAGAGATCTGGTGTTGGTG+TGG 0.606899 tig0024801:+76339 Msa1420380:three_prime_UTR
GTCTGAACCTGAAGATAACA+AGG 0.608807 tig0024801:+74563 Msa1420380:CDS
TGAAGCACTGCATATTGATG+AGG 0.609628 tig0024801:-75619 Msa1420390:CDS
GGAAACGGTGGCGGAGACAA+CGG 0.613681 tig0024801:+76228 Msa1420380:three_prime_UTR
CTTAGGTAGACGGAAAGGGA+CGG 0.618125 tig0024801:-75873 Msa1420390:CDS
AGTCAAATCAGATTAAGGAA+AGG 0.621653 tig0024801:+74517 Msa1420380:CDS
CATTTCATCCCTGTAATTAG+GGG 0.623909 tig0024801:+75250 Msa1420380:three_prime_UTR
CAGAGAAGTGAAGTGAAGGA+GGG 0.625228 tig0024801:-75461 None:intergenic
GACAACGGAGAGAGAATGGA+CGG 0.627558 tig0024801:+76265 Msa1420380:three_prime_UTR
ACTCACATGTGATGACTCAA+GGG 0.627657 tig0024801:+75180 Msa1420380:three_prime_UTR
AGTAAGTGAAGGATCAGGGA+GGG 0.629875 tig0024801:-75936 Msa1420390:CDS
GTGTCCCCAACAACCTCCGG+CGG 0.630049 tig0024801:+75818 Msa1420380:three_prime_UTR
TCGGCGGTTTCCACCGCCGG+AGG 0.630883 tig0024801:-75850 Msa1420390:CDS
TGGGAGGATTAGTAAGCTCA+GGG 0.635180 tig0024801:-75308 None:intergenic
GTGTTGATTGAAGAGTTCGA+TGG 0.635725 tig0024801:+76296 Msa1420380:three_prime_UTR
AATCTGAAAGACATGAGTCA+AGG 0.637514 tig0024801:+75050 Msa1420380:CDS
GATCTATCCACAACCACAAG+GGG 0.639953 tig0024801:-75518 None:intergenic
GGTAGACGGAAAGGGACGGT+CGG 0.642297 tig0024801:-75869 Msa1420390:CDS
GATGAACATAGGGGTAGACA+GGG 0.645678 tig0024801:-75425 None:intergenic
ACAAAAGCAACAACAAATGG+TGG 0.646145 tig0024801:+75073 Msa1420380:CDS
ACGAAACTACAAGTAAGTGA+AGG 0.651561 tig0024801:-75947 Msa1420390:CDS
GGAATCAGAATCGAAATCGT+CGG 0.654540 tig0024801:-75902 Msa1420390:CDS
GGATCTATCCACAACCACAA+GGG 0.657598 tig0024801:-75519 None:intergenic
GTGGCGGAGACAACGGAGTG+CGG 0.658208 tig0024801:+76235 Msa1420380:three_prime_UTR
AATTACAGGGATGAAATGGG+AGG 0.666408 tig0024801:-75324 None:intergenic
AGAAGAAAAGAAGAAGACAG+GGG 0.685426 tig0024801:+76418 Msa1420380:three_prime_UTR
ATAATAATAAGTCAAGAACA+TGG 0.691937 tig0024801:+75101 Msa1420380:CDS
ACATGGAAGAGATCTGGTGT+TGG 0.696196 tig0024801:+76334 Msa1420380:three_prime_UTR
GGTGTTGGTGGTGATAGACA+TGG 0.701088 tig0024801:+76317 Msa1420380:three_prime_UTR
TTCTCATGTTTCCGGCACGG+TGG 0.703341 tig0024801:+75952 Msa1420380:three_prime_UTR
GTGGAGGGTAATTAGTGACG+TGG 0.731911 tig0024801:-76502 None:intergenic
AAGTAAGTGAAGGATCAGGG+AGG 0.735147 tig0024801:-75937 Msa1420390:CDS
ATGAACATAGGGGTAGACAG+GGG 0.756859 tig0024801:-75424 None:intergenic
GTTGTTGGGGACACAAACGA+CGG 0.759292 tig0024801:-75828 Msa1420390:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTAATAATAATTTATGAT+TGG + tig0024801:75006-75025 Msa1420380:intron 10.0%
!! ATTATTAATCACTTTAAATT+TGG + tig0024801:75325-75344 Msa1420380:three_prime_UTR 10.0%
!! TATAAAGAATCAAAAGAAAA+AGG - tig0024801:74356-74375 None:intergenic 15.0%
!!! AGAACAAAATAATCTTTTAA+GGG - tig0024801:74389-74408 None:intergenic 15.0%
!!! AAGAACAAAATAATCTTTTA+AGG - tig0024801:74390-74409 None:intergenic 15.0%
!!! TACACATTGATTATTTTTTT+TGG + tig0024801:74762-74781 Msa1420380:intron 15.0%
!! GATAATGCATAATATATATA+AGG - tig0024801:74801-74820 None:intergenic 15.0%
!! TTATGTACATTAACTAATTA+AGG + tig0024801:74859-74878 Msa1420380:intron 15.0%
!! TAAAGTGATTAATAATTACA+GGG - tig0024801:75321-75340 None:intergenic 15.0%
!! TTAAAGTGATTAATAATTAC+AGG - tig0024801:75322-75341 None:intergenic 15.0%
!! AAAAAGAAAAGAAAAGAAAT+TGG + tig0024801:76112-76131 Msa1420380:three_prime_UTR 15.0%
!! TTAATGATAAAACAGCTAAT+AGG + tig0024801:74541-74560 Msa1420380:CDS 20.0%
!! CATAATATATATAAGGTTTG+AGG - tig0024801:74794-74813 None:intergenic 20.0%
!! ATAATAATAAGTCAAGAACA+TGG + tig0024801:75101-75120 Msa1420380:CDS 20.0%
!!! TAACAATTTTTACATCACTA+AGG + tig0024801:75720-75739 Msa1420380:three_prime_UTR 20.0%
!!! ATCAGCAAATTTTTGTAATT+AGG + tig0024801:76506-76525 Msa1420380:three_prime_UTR 20.0%
! GTTGCTATAAATATTCTAGT+AGG + tig0024801:73912-73931 Msa1420380:five_prime_UTR 25.0%
! AAATAATAAGGAAGATGCAA+CGG - tig0024801:73953-73972 None:intergenic 25.0%
! GAGGAAGGATATAAATAATA+AGG - tig0024801:73965-73984 None:intergenic 25.0%
! AAAAAGAAGTGAGAGATAAT+GGG + tig0024801:73997-74016 Msa1420380:exon 25.0%
!!! TTGAGTCTTTATGTTGTTTT+TGG + tig0024801:74416-74435 Msa1420380:intron 25.0%
! ACAAAAGTCAAATCAGATTA+AGG + tig0024801:74512-74531 Msa1420380:CDS 25.0%
! TATAGTTACTGTTCATAAGA+AGG - tig0024801:74716-74735 None:intergenic 25.0%
! TACATTAACTAATTAAGGTC+TGG + tig0024801:74864-74883 Msa1420380:intron 25.0%
!! GGTCTGGTTATAAATTTTAT+TGG + tig0024801:74880-74899 Msa1420380:intron 25.0%
!!! ATGATTGGTTCATTATTTTC+AGG + tig0024801:75021-75040 Msa1420380:intron 25.0%
! ATCTGTGGGAAATTAAATTA+AGG + tig0024801:75217-75236 Msa1420380:three_prime_UTR 25.0%
! TCTGTGGGAAATTAAATTAA+GGG + tig0024801:75218-75237 Msa1420380:three_prime_UTR 25.0%
! AATAATTACAGGGATGAAAT+GGG - tig0024801:75311-75330 None:intergenic 25.0%
! TAATAATTACAGGGATGAAA+TGG - tig0024801:75312-75331 None:intergenic 25.0%
! TAAGGGCAAAATTCTTAATT+AGG - tig0024801:75352-75371 None:intergenic 25.0%
!!! ATTTTGAGAAGATGAACATA+GGG - tig0024801:75419-75438 None:intergenic 25.0%
!!! TTTTTTAAAGTTCTTTTGGG+AGG - tig0024801:75479-75498 None:intergenic 25.0%
!!! GGGTTTTTTAAAGTTCTTTT+GGG - tig0024801:75482-75501 None:intergenic 25.0%
!!! AAAACCATGTGTTTTTTTGT+AGG - tig0024801:75529-75548 Msa1420390:CDS 25.0%
!!! GAAATTGGTGTTTTAGAAAT+TGG + tig0024801:76069-76088 Msa1420380:three_prime_UTR 25.0%
! ACAACACAATTCAATTTCTT+TGG + tig0024801:76207-76226 Msa1420380:three_prime_UTR 25.0%
!! CAATTCAATTTCTTTGGAAA+CGG + tig0024801:76213-76232 Msa1420380:three_prime_UTR 25.0%
!!! ATTACAAAAATTTGCTGATG+TGG - tig0024801:76505-76524 None:intergenic 25.0%
! TTTGACTCTTTGAAAACTAG+TGG + tig0024801:73875-73894 Msa1420380:five_prime_UTR 30.0%
! TCTTTTTCTTCTTCAAGAAG+AGG - tig0024801:73984-74003 None:intergenic 30.0%
GAAAAAGAAGTGAGAGATAA+TGG + tig0024801:73996-74015 Msa1420380:exon 30.0%
!!! TTTTGAAGTGCATAATGTTG+TGG - tig0024801:74157-74176 None:intergenic 30.0%
!!! TATGTTGTTTTTGGTTTCAG+AGG + tig0024801:74425-74444 Msa1420380:intron 30.0%
AGTCAAATCAGATTAAGGAA+AGG + tig0024801:74517-74536 Msa1420380:CDS 30.0%
! AGATTTACCTTGTTATCTTC+AGG - tig0024801:74573-74592 None:intergenic 30.0%
AGATTCAATCAACATGGATT+TGG - tig0024801:74627-74646 None:intergenic 30.0%
AAGAACATGAATATCTTCAG+TGG + tig0024801:74649-74668 Msa1420380:intron 30.0%
!! TAAGAGAGAGATTTGTTGAA+TGG + tig0024801:74982-75001 Msa1420380:intron 30.0%
!! TTTTGAGAAGATGAACATAG+GGG - tig0024801:75418-75437 None:intergenic 30.0%
!! GATTTTGAGAAGATGAACAT+AGG - tig0024801:75420-75439 None:intergenic 30.0%
!!! GGGGTTTTTTAAAGTTCTTT+TGG - tig0024801:75483-75502 None:intergenic 30.0%
!!! CATGTGTTTTTTTGTAGGTA+TGG - tig0024801:75524-75543 None:intergenic 30.0%
CATACCTACAAAAAAACACA+TGG + tig0024801:75522-75541 Msa1420380:three_prime_UTR 30.0%
AAGGAGAAGTAGTTGAAAAA+GGG - tig0024801:75570-75589 Msa1420390:CDS 30.0%
TTGATGAGGAAATCAATTGA+AGG - tig0024801:75589-75608 Msa1420390:CDS 30.0%
!! CTTCTTCATTTTTCACTTGT+AGG + tig0024801:75626-75645 Msa1420380:three_prime_UTR 30.0%
!! CATTGGAAAATTCTAGTTCA+GGG - tig0024801:75653-75672 Msa1420390:intron 30.0%
!! ACATTGGAAAATTCTAGTTC+AGG - tig0024801:75654-75673 Msa1420390:intron 30.0%
!! GTTGTTGTTGTGTTTTACAT+TGG - tig0024801:75670-75689 Msa1420390:intron 30.0%
AATGACCCAAATGCAAAATA+TGG - tig0024801:76013-76032 None:intergenic 30.0%
! ATTGTTTCTTGTGTCAAGTA+AGG + tig0024801:76030-76049 Msa1420380:three_prime_UTR 30.0%
! ACTTGTTGGGATTTAGAAAT+TGG + tig0024801:76054-76073 Msa1420380:three_prime_UTR 30.0%
! ACAATACAAGGTTACTTTGA+TGG + tig0024801:76178-76197 Msa1420380:three_prime_UTR 30.0%
AAAGAAGAAAAGAAGAAGAC+AGG + tig0024801:76416-76435 Msa1420380:three_prime_UTR 30.0%
AAGAAGAAAAGAAGAAGACA+GGG + tig0024801:76417-76436 Msa1420380:three_prime_UTR 30.0%
TTTCTTCTTCAAGAAGAGGA+AGG - tig0024801:73980-73999 None:intergenic 35.0%
AACCAAATGGCAAAGAAGAA+AGG + tig0024801:74061-74080 Msa1420380:CDS 35.0%
AACCTTTCTTCTTTGCCATT+TGG - tig0024801:74066-74085 None:intergenic 35.0%
GCAAAGAAGAAAGGTTGTAT+TGG + tig0024801:74070-74089 Msa1420380:CDS 35.0%
! TTTGGTTTCAGAGGTTACAA+AGG + tig0024801:74434-74453 Msa1420380:intron 35.0%
TTGGTTTCAGAGGTTACAAA+GGG + tig0024801:74435-74454 Msa1420380:intron 35.0%
!!! TTTGACTTTTGTGCTGATTC+TGG - tig0024801:74504-74523 None:intergenic 35.0%
TCTTGCAGATTCAATCAACA+TGG - tig0024801:74633-74652 None:intergenic 35.0%
TACTGTTCATAAGAAGGAGT+TGG - tig0024801:74710-74729 None:intergenic 35.0%
AATCTGAAAGACATGAGTCA+AGG + tig0024801:75050-75069 Msa1420380:CDS 35.0%
AGGACAAAAGCAACAACAAA+TGG + tig0024801:75070-75089 Msa1420380:CDS 35.0%
ACAAAAGCAACAACAAATGG+TGG + tig0024801:75073-75092 Msa1420380:CDS 35.0%
TCAGTGATGCTTTGTAAAGA+GGG - tig0024801:75130-75149 None:intergenic 35.0%
TTCAGTGATGCTTTGTAAAG+AGG - tig0024801:75131-75150 None:intergenic 35.0%
GATTCAAAACTCATATCAGC+TGG - tig0024801:75158-75177 None:intergenic 35.0%
ACATGATTGCTTAGATCTGT+GGG + tig0024801:75203-75222 Msa1420380:three_prime_UTR 35.0%
TGCATTTCATCCCTGTAATT+AGG + tig0024801:75248-75267 Msa1420380:three_prime_UTR 35.0%
GCATTTCATCCCTGTAATTA+GGG + tig0024801:75249-75268 Msa1420380:three_prime_UTR 35.0%
CATTTCATCCCTGTAATTAG+GGG + tig0024801:75250-75269 Msa1420380:three_prime_UTR 35.0%
TCAAATGACCCCTAATTACA+GGG - tig0024801:75261-75280 None:intergenic 35.0%
TTCAAATGACCCCTAATTAC+AGG - tig0024801:75262-75281 None:intergenic 35.0%
CGTGTCTCAGATTTCATTAA+GGG - tig0024801:75369-75388 None:intergenic 35.0%
ACGTGTCTCAGATTTCATTA+AGG - tig0024801:75370-75389 None:intergenic 35.0%
!! AAAGTTCTTTTGGGAGGAAA+TGG - tig0024801:75473-75492 None:intergenic 35.0%
! GAACTTTAAAAAACCCCTTG+TGG + tig0024801:75486-75505 Msa1420380:three_prime_UTR 35.0%
GAAGTAGTTGAAAAAGGGTT+TGG - tig0024801:75565-75584 Msa1420390:CDS 35.0%
GAAGGAGAAGTAGTTGAAAA+AGG - tig0024801:75571-75590 Msa1420390:CDS 35.0%
TACAAGTAAGTGAAGGATCA+GGG - tig0024801:75924-75943 Msa1420390:CDS 35.0%
ACGAAACTACAAGTAAGTGA+AGG - tig0024801:75931-75950 Msa1420390:CDS 35.0%
CCAAATGCAAAATATGGCTA+CGG - tig0024801:76007-76026 Msa1420390:CDS 35.0%
! CGTAGCCATATTTTGCATTT+GGG + tig0024801:76005-76024 Msa1420380:three_prime_UTR 35.0%
! TGTCAAGTAAGGAACTTGTT+GGG + tig0024801:76041-76060 Msa1420380:three_prime_UTR 35.0%
GATTCGTCTTCAACAATACA+AGG + tig0024801:76166-76185 Msa1420380:three_prime_UTR 35.0%
! TTCAATTTCTTTGGAAACGG+TGG + tig0024801:76216-76235 Msa1420380:three_prime_UTR 35.0%
AGACTGAAAAAGAGGAAAGA+AGG + tig0024801:76368-76387 Msa1420380:three_prime_UTR 35.0%
TGAAAAAGAGGAAAGAAGGA+CGG + tig0024801:76372-76391 Msa1420380:three_prime_UTR 35.0%
AGAAGAAAAGAAGAAGACAG+GGG + tig0024801:76418-76437 Msa1420380:three_prime_UTR 35.0%
!! CAAAAATTTGCTGATGTGGA+GGG - tig0024801:76501-76520 None:intergenic 35.0%
!! ACAAAAATTTGCTGATGTGG+AGG - tig0024801:76502-76521 None:intergenic 35.0%
CTTGTGATACAGCAACCAAA+TGG + tig0024801:74048-74067 Msa1420380:CDS 40.0%
ATCACTTCACTAGCAGTAAC+TGG - tig0024801:74096-74115 None:intergenic 40.0%
GAAGAGCAACATAGTGATCA+GGG - tig0024801:74128-74147 None:intergenic 40.0%
AGAAGAGCAACATAGTGATC+AGG - tig0024801:74129-74148 None:intergenic 40.0%
! CCAAGCAAAAGTGTATCTGT+TGG - tig0024801:74273-74292 None:intergenic 40.0%
! CCAACAGATACACTTTTGCT+TGG + tig0024801:74270-74289 Msa1420380:CDS 40.0%
TATAGACTCATCTCAGCTCA+AGG + tig0024801:74300-74319 Msa1420380:CDS 40.0%
! AGAGGTTACAAAGGGTATGT+GGG + tig0024801:74443-74462 Msa1420380:intron 40.0%
GTCTGAACCTGAAGATAACA+AGG + tig0024801:74563-74582 Msa1420380:CDS 40.0%
CCTTGCTACTACAAAGTCAA+TGG - tig0024801:74932-74951 None:intergenic 40.0%
!! CCATTGACTTTGTAGTAGCA+AGG + tig0024801:74929-74948 Msa1420380:intron 40.0%
TACTCACATGTGATGACTCA+AGG + tig0024801:75179-75198 Msa1420380:three_prime_UTR 40.0%
ACTCACATGTGATGACTCAA+GGG + tig0024801:75180-75199 Msa1420380:three_prime_UTR 40.0%
GACATGATTGCTTAGATCTG+TGG + tig0024801:75202-75221 Msa1420380:three_prime_UTR 40.0%
AATTACAGGGATGAAATGGG+AGG - tig0024801:75308-75327 None:intergenic 40.0%
TAAAAAACCCCTTGTGGTTG+TGG + tig0024801:75492-75511 Msa1420380:three_prime_UTR 40.0%
! GTTGAAAAAGGGTTTGGTAG+TGG - tig0024801:75559-75578 Msa1420390:CDS 40.0%
!! TGAAGCACTGCATATTGATG+AGG - tig0024801:75603-75622 Msa1420390:CDS 40.0%
ACAACAACAACGTCATCTTC+TGG + tig0024801:75679-75698 Msa1420380:three_prime_UTR 40.0%
CAACAACAACGTCATCTTCT+GGG + tig0024801:75680-75699 Msa1420380:three_prime_UTR 40.0%
GGAATCAGAATCGAAATCGT+CGG - tig0024801:75886-75905 Msa1420390:CDS 40.0%
GACGATTTCGATTCTGATTC+CGG + tig0024801:75885-75904 Msa1420380:three_prime_UTR 40.0%
CTACAAGTAAGTGAAGGATC+AGG - tig0024801:75925-75944 Msa1420390:CDS 40.0%
GTTTCGTCTTCTCATGTTTC+CGG + tig0024801:75944-75963 Msa1420380:three_prime_UTR 40.0%
! CCGTAGCCATATTTTGCATT+TGG + tig0024801:76004-76023 Msa1420380:three_prime_UTR 40.0%
GTGTCAAGTAAGGAACTTGT+TGG + tig0024801:76040-76059 Msa1420380:three_prime_UTR 40.0%
TGAAGACGAATCTGTGAAAG+AGG - tig0024801:76158-76177 None:intergenic 40.0%
! AATTTCTTTGGAAACGGTGG+CGG + tig0024801:76219-76238 Msa1420380:three_prime_UTR 40.0%
!! GTGTTGATTGAAGAGTTCGA+TGG + tig0024801:76296-76315 Msa1420380:three_prime_UTR 40.0%
ATTGAAGAGTTCGATGGTGT+TGG + tig0024801:76302-76321 Msa1420380:three_prime_UTR 40.0%
TGATAGACATGGAAGAGATC+TGG + tig0024801:76328-76347 Msa1420380:three_prime_UTR 40.0%
GAAAAAGAGGAAAGAAGGAC+GGG + tig0024801:76373-76392 Msa1420380:three_prime_UTR 40.0%
!! TGGCGTCTGTTTAATTTGCT+GGG - tig0024801:76466-76485 None:intergenic 40.0%
ACTAGTGGTAAGAGAGTGAG+TGG + tig0024801:73890-73909 Msa1420380:five_prime_UTR 45.0%
! CAGAGGTTACAAAGGGTATG+TGG + tig0024801:74442-74461 Msa1420380:intron 45.0%
! TGGGAGGATTAGTAAGCTCA+GGG - tig0024801:75292-75311 None:intergenic 45.0%
! ATGGGAGGATTAGTAAGCTC+AGG - tig0024801:75293-75312 None:intergenic 45.0%
ATGAACATAGGGGTAGACAG+GGG - tig0024801:75408-75427 None:intergenic 45.0%
GATGAACATAGGGGTAGACA+GGG - tig0024801:75409-75428 None:intergenic 45.0%
AGATGAACATAGGGGTAGAC+AGG - tig0024801:75410-75429 None:intergenic 45.0%
CAGAGAAGTGAAGTGAAGGA+GGG - tig0024801:75445-75464 None:intergenic 45.0%
GTTCCAGAGAAGTGAAGTGA+AGG - tig0024801:75449-75468 None:intergenic 45.0%
GATCTATCCACAACCACAAG+GGG - tig0024801:75502-75521 None:intergenic 45.0%
GGATCTATCCACAACCACAA+GGG - tig0024801:75503-75522 None:intergenic 45.0%
TGGATCTATCCACAACCACA+AGG - tig0024801:75504-75523 None:intergenic 45.0%
TGAGCTTAGGTAGACGGAAA+GGG - tig0024801:75861-75880 Msa1420390:CDS 45.0%
AGTAAGTGAAGGATCAGGGA+GGG - tig0024801:75920-75939 Msa1420390:CDS 45.0%
AAGTAAGTGAAGGATCAGGG+AGG - tig0024801:75921-75940 Msa1420390:CDS 45.0%
ACATGGAAGAGATCTGGTGT+TGG + tig0024801:76334-76353 Msa1420380:three_prime_UTR 45.0%
GGTGGAAGAGACTGAAAAAG+AGG + tig0024801:76360-76379 Msa1420380:three_prime_UTR 45.0%
AGGACGGGTAAGAAGAAAAG+AGG + tig0024801:76388-76407 Msa1420380:three_prime_UTR 45.0%
GGACGGGTAAGAAGAAAAGA+GGG + tig0024801:76389-76408 Msa1420380:three_prime_UTR 45.0%
GGGTAAGAAGAAAAGAGGGA+GGG + tig0024801:76393-76412 Msa1420380:three_prime_UTR 45.0%
!! GTGGCGTCTGTTTAATTTGC+TGG - tig0024801:76467-76486 None:intergenic 45.0%
CCAGAGAAGTGAAGTGAAGG+AGG - tig0024801:75446-75465 None:intergenic 50.0%
CCTCCTTCACTTCACTTCTC+TGG + tig0024801:75443-75462 Msa1420380:three_prime_UTR 50.0%
GTTGTTGGGGACACAAACGA+CGG - tig0024801:75812-75831 Msa1420390:intron 50.0%
TTTGTGTCCCCAACAACCTC+CGG + tig0024801:75815-75834 Msa1420380:three_prime_UTR 50.0%
CTTAGGTAGACGGAAAGGGA+CGG - tig0024801:75857-75876 Msa1420390:CDS 50.0%
GTGAGCTTAGGTAGACGGAA+AGG - tig0024801:75862-75881 Msa1420390:CDS 50.0%
TCGGAGTGAGCTTAGGTAGA+CGG - tig0024801:75867-75886 Msa1420390:CDS 50.0%
GAAATCGTCGGAGTGAGCTT+AGG - tig0024801:75874-75893 Msa1420390:CDS 50.0%
GTCTTCTCATGTTTCCGGCA+CGG + tig0024801:75949-75968 Msa1420380:three_prime_UTR 50.0%
AGGAGACAACGGAGAGAGAA+TGG + tig0024801:76261-76280 Msa1420380:three_prime_UTR 50.0%
GACAACGGAGAGAGAATGGA+CGG + tig0024801:76265-76284 Msa1420380:three_prime_UTR 50.0%
! GAAGAGTTCGATGGTGTTGG+TGG + tig0024801:76305-76324 Msa1420380:three_prime_UTR 50.0%
!! GGTGTTGGTGGTGATAGACA+TGG + tig0024801:76317-76336 Msa1420380:three_prime_UTR 50.0%
!! GAAGAGATCTGGTGTTGGTG+TGG + tig0024801:76339-76358 Msa1420380:three_prime_UTR 50.0%
CGGGTAAGAAGAAAAGAGGG+AGG + tig0024801:76392-76411 Msa1420380:three_prime_UTR 50.0%
GTGGAGGGTAATTAGTGACG+TGG - tig0024801:76486-76505 None:intergenic 50.0%
TCAGGGAGGGAACTCAGAAC+CGG - tig0024801:75907-75926 Msa1420390:CDS 55.0%
TTCTCATGTTTCCGGCACGG+TGG + tig0024801:75952-75971 Msa1420380:three_prime_UTR 55.0%
GGAGAGAGAATGGACGGTGT+TGG + tig0024801:76271-76290 Msa1420380:three_prime_UTR 55.0%
!! GAGATCTGGTGTTGGTGTGG+TGG + tig0024801:76342-76361 Msa1420380:three_prime_UTR 55.0%
TTCCACCGCCGGAGGTTGTT+GGG - tig0024801:75826-75845 Msa1420390:intron 60.0%
TTTCCACCGCCGGAGGTTGT+TGG - tig0024801:75827-75846 Msa1420390:intron 60.0%
GGTAGACGGAAAGGGACGGT+CGG - tig0024801:75853-75872 Msa1420390:CDS 60.0%
TCATGTTTCCGGCACGGTGG+CGG + tig0024801:75955-75974 Msa1420380:three_prime_UTR 60.0%
!! GGAAACGGTGGCGGAGACAA+CGG + tig0024801:76228-76247 Msa1420380:three_prime_UTR 60.0%
GTGTCCCCAACAACCTCCGG+CGG + tig0024801:75818-75837 Msa1420380:three_prime_UTR 65.0%
TCCACCGCCGGAGGTTGTTG+GGG - tig0024801:75825-75844 Msa1420390:intron 65.0%
AGACGGAAAGGGACGGTCGG+CGG - tig0024801:75850-75869 Msa1420390:CDS 65.0%
! GTGGCGGAGACAACGGAGTG+CGG + tig0024801:76235-76254 Msa1420380:three_prime_UTR 65.0%
GAGTGCGGCGGAGGAGACAA+CGG + tig0024801:76250-76269 Msa1420380:three_prime_UTR 65.0%
TCCCCAACAACCTCCGGCGG+TGG + tig0024801:75821-75840 Msa1420380:three_prime_UTR 70.0%
! TGTTTCCGGCACGGTGGCGG+CGG + tig0024801:75958-75977 Msa1420380:three_prime_UTR 70.0%
GCGGAGACAACGGAGTGCGG+CGG + tig0024801:76238-76257 Msa1420380:three_prime_UTR 70.0%
GAGACAACGGAGTGCGGCGG+AGG + tig0024801:76241-76260 Msa1420380:three_prime_UTR 70.0%
TCGGCGGTTTCCACCGCCGG+AGG - tig0024801:75834-75853 Msa1420390:CDS 75.0%
CGGTCGGCGGTTTCCACCGC+CGG - tig0024801:75837-75856 Msa1420390:CDS 75.0%
! GGTGGCGGCGGTGGTGGTGA+TGG + tig0024801:75970-75989 Msa1420380:three_prime_UTR 75.0%
!! TTCCGGCACGGTGGCGGCGG+TGG + tig0024801:75961-75980 Msa1420380:three_prime_UTR 80.0%
! CACCACCGCCGCCACCGTGC+CGG - tig0024801:75966-75985 Msa1420390:CDS 80.0%
!! CGGCACGGTGGCGGCGGTGG+TGG + tig0024801:75964-75983 Msa1420380:three_prime_UTR 85.0%
Chromosome Type Strat End Strand Name
tig0024801 gene 73861 76534 73861 ID=Msa1420380;Name=Msa1420380
tig0024801 mRNA 73861 76534 73861 ID=Msa1420380-mRNA-1;Parent=Msa1420380;Name=Msa1420380-mRNA-1;_AED=0.10;_eAED=0.10;_QI=154|1|1|1|1|1|3|1372|188
tig0024801 exon 73861 74321 73861 ID=Msa1420380-mRNA-1:exon:1427;Parent=Msa1420380-mRNA-1
tig0024801 exon 74445 74584 74445 ID=Msa1420380-mRNA-1:exon:1428;Parent=Msa1420380-mRNA-1
tig0024801 exon 75043 76534 75043 ID=Msa1420380-mRNA-1:exon:1429;Parent=Msa1420380-mRNA-1
tig0024801 five_prime_UTR 73861 74014 73861 ID=Msa1420380-mRNA-1:five_prime_utr;Parent=Msa1420380-mRNA-1
tig0024801 CDS 74015 74321 74015 ID=Msa1420380-mRNA-1:cds;Parent=Msa1420380-mRNA-1
tig0024801 CDS 74445 74584 74445 ID=Msa1420380-mRNA-1:cds;Parent=Msa1420380-mRNA-1
tig0024801 CDS 75043 75162 75043 ID=Msa1420380-mRNA-1:cds;Parent=Msa1420380-mRNA-1
tig0024801 three_prime_UTR 75163 76534 75163 ID=Msa1420380-mRNA-1:three_prime_utr;Parent=Msa1420380-mRNA-1
Gene Sequence

>Msa1420380

ATGGGAAATTGTCAAGCTATTGATACAGCAACTCTTGTGATACAGCAACCAAATGGCAAAGAAGAAAGGTTGTATTGGCCAGTTACTGCTAGTGAAGTGATGAAGACACACCCTGATCACTATGTTGCTCTTCTTATCTCCACAACATTATGCACTTCAAAAGACAAAGAAAATTGTTCAAATAAGAGCAGCGACAACAACAACGACGACAACAACAACAACAAAGTTCGTATTACACGTATAAAACTTCTTAAGCCAACAGATACACTTTTGCTTGGTCAAGTTTATAGACTCATCTCAGCTCAAGAGGTTACAAAGGGTATGTGGGCTAAGAAACAAGCAAAGATGAAGAGAAACTCTTTGCCAGAATCAGCACAAAAGTCAAATCAGATTAAGGAAAGGATTAATGATAAAACAGCTAATAGGTCTGAACCTGAAGATAACAAGGAAACAAAATCTGAAAGACATGAGTCAAGGACAAAAGCAACAACAAATGGTGGCAATCATAATAATAAGTCAAGAACATGGCAACCCTCTTTACAAAGCATCACTGAATCTGCCAGCTGA
Protein sequence

>Msa1420380

MGNCQAIDTATLVIQQPNGKEERLYWPVTASEVMKTHPDHYVALLISTTLCTSKDKENCSNKSSDNNNDDNNNNKVRITRIKLLKPTDTLLLGQVYRLISAQEVTKGMWAKKQAKMKRNSLPESAQKSNQIKERINDKTANRSEPEDNKETKSERHESRTKATTNGGNHNNKSRTWQPSLQSITESAS*