AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1429840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1429840 MtrunA17_Chr3g0137821 97.818 825 15 1 1 825 1 822 0.0 1686
Msa1429840 MtrunA17_Chr3g0137791 70.286 838 224 12 1 825 1 826 0.0 1198
Msa1429840 MtrunA17_Chr4g0003411 64.388 834 262 12 1 825 1 808 0.0 1085
Msa1429840 MtrunA17_Chr2g0333021 63.057 831 272 11 4 825 5 809 0.0 1040
Msa1429840 MtrunA17_Chr5g0398161 44.807 828 420 16 4 814 37 844 0.0 686
Msa1429840 MtrunA17_Chr7g0246481 44.699 830 417 18 4 814 298 1104 0.0 685
Msa1429840 MtrunA17_Chr8g0380631 44.983 867 405 23 2 825 12 849 0.0 680
Msa1429840 MtrunA17_Chr3g0143471 44.091 880 407 24 5 825 13 866 0.0 680
Msa1429840 MtrunA17_Chr5g0398171 43.943 842 401 19 4 814 37 838 0.0 674
Msa1429840 MtrunA17_Chr8g0350951 43.593 835 424 17 4 814 228 1039 0.0 668
Msa1429840 MtrunA17_Chr5g0406161 42.207 879 428 18 5 825 16 872 0.0 665
Msa1429840 MtrunA17_Chr2g0330431 43.534 781 379 18 53 825 30 756 0.0 622
Msa1429840 MtrunA17_Chr8g0341931 41.396 831 398 21 2 825 5 753 0.0 588
Msa1429840 MtrunA17_Chr1g0192401 32.500 240 123 10 510 740 733 942 2.60e-21 100
Msa1429840 MtrunA17_Chr1g0192401 30.544 239 123 10 211 435 350 559 1.40e-19 94.4
Msa1429840 MtrunA17_Chr2g0295411 33.333 201 102 5 510 705 783 956 2.75e-18 90.5
Msa1429840 MtrunA17_Chr2g0295411 30.453 243 119 11 211 435 376 586 4.84e-18 89.7
Msa1429840 MtrunA17_Chr5g0406151 51.351 74 30 2 326 399 12 79 6.57e-18 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1429840 AT3G15730.1 62.560 828 284 12 3 825 4 810 0.0 1060
Msa1429840 AT1G52570.1 62.031 827 288 12 4 825 5 810 0.0 1040
Msa1429840 AT1G52570.3 62.031 827 288 12 4 825 5 810 0.0 1040
Msa1429840 AT1G52570.2 62.031 827 288 12 4 825 5 810 0.0 1040
Msa1429840 AT5G25370.1 60.555 829 306 14 4 825 6 820 0.0 1003
Msa1429840 AT5G25370.3 60.555 829 306 14 4 825 6 820 0.0 1003
Msa1429840 AT5G25370.2 60.555 829 306 14 4 825 6 820 0.0 1003
Msa1429840 AT5G25370.4 62.850 751 263 12 81 825 5 745 0.0 954
Msa1429840 AT2G42010.1 44.391 829 418 17 4 814 270 1073 0.0 687
Msa1429840 AT4G11830.2 44.153 838 423 20 1 814 30 846 0.0 685
Msa1429840 AT4G11830.1 45.817 777 383 16 59 814 55 814 0.0 684
Msa1429840 AT2G42010.2 43.361 851 420 17 4 814 270 1098 0.0 680
Msa1429840 AT4G11840.1 43.705 842 417 20 3 814 42 856 0.0 673
Msa1429840 AT4G00240.1 44.058 833 415 19 4 814 114 917 0.0 671
Msa1429840 AT4G11850.1 44.019 836 419 19 3 814 38 848 0.0 668
Msa1429840 AT4G35790.2 43.886 875 410 19 4 825 11 857 0.0 668
Msa1429840 AT4G00240.3 45.349 774 386 15 59 814 11 765 0.0 667
Msa1429840 AT4G00240.2 45.349 774 386 15 59 814 128 882 0.0 666
Msa1429840 AT4G35790.1 43.567 886 408 20 4 825 11 868 0.0 661
Msa1429840 AT4G11840.2 43.214 840 394 19 3 814 42 826 0.0 655
Msa1429840 AT1G55180.2 41.000 800 397 23 39 825 3 740 0.0 582
Msa1429840 AT1G55180.1 41.184 794 392 23 45 825 31 762 0.0 581
Msa1429840 AT4G35790.3 42.857 693 323 15 4 643 11 683 1.80e-168 504
Msa1429840 AT4G11830.4 42.697 623 325 15 1 609 30 634 1.79e-160 481
Msa1429840 AT4G11830.3 42.904 606 314 15 1 592 30 617 2.15e-156 471
Msa1429840 AT3G05630.1 34.413 247 125 9 512 750 714 931 2.16e-24 110
Msa1429840 AT3G05630.1 32.218 239 119 10 211 435 363 572 2.78e-19 94.0
Msa1429840 AT3G16785.6 33.182 220 101 9 231 435 389 577 6.14e-20 95.9
Msa1429840 AT3G16785.6 36.453 203 95 7 509 705 756 930 9.27e-20 95.5
Msa1429840 AT3G16785.5 33.182 220 101 9 231 435 389 577 6.14e-20 95.9
Msa1429840 AT3G16785.5 36.453 203 95 7 509 705 756 930 9.27e-20 95.5
Msa1429840 AT3G16785.4 33.182 220 101 9 231 435 389 577 6.19e-20 96.3
Msa1429840 AT3G16785.4 36.453 203 95 7 509 705 756 930 1.06e-19 95.5
Msa1429840 AT3G16785.1 33.182 220 101 9 231 435 389 577 6.72e-20 95.9
Msa1429840 AT3G16785.1 36.453 203 95 7 509 705 756 930 9.54e-20 95.5
Msa1429840 AT3G16785.3 33.182 220 101 9 231 435 389 577 7.13e-20 95.9
Msa1429840 AT3G16785.3 36.453 203 95 7 509 705 756 930 9.79e-20 95.5
Msa1429840 AT3G16785.2 33.182 220 101 9 231 435 389 577 7.13e-20 95.9
Msa1429840 AT3G16785.2 36.453 203 95 7 509 705 756 930 9.79e-20 95.5

Find 203 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGTCTCATTCCATTTC+GGG 0.167631 tig0026572:-30966 None:intergenic
CTACTTTCTGTTGGCTTGTT+TGG 0.177927 tig0026572:-31480 None:intergenic
GATACAATGGCTTTGATTCT+TGG 0.200605 tig0026572:+33498 Msa1429840:CDS
TTTACGCATTCCACACTTTC+AGG 0.207301 tig0026572:-33327 None:intergenic
ATAGAAGCACCTGGGAAATT+TGG 0.213465 tig0026572:-31909 None:intergenic
CCAAATGAAGTTATTGAATT+AGG 0.215359 tig0026572:+32200 Msa1429840:CDS
TTCCACCATCCATTGATCTT+TGG 0.219999 tig0026572:-33155 None:intergenic
GTTGCAAGCTGGATGTAATT+TGG 0.224934 tig0026572:+30380 Msa1429840:CDS
CTAGGACTAGGTGGTAAGTA+TGG 0.233733 tig0026572:-33544 None:intergenic
CAACCTATCAACTTCACTTA+TGG 0.246044 tig0026572:-30361 None:intergenic
TACTTTCTGTTGGCTTGTTT+GGG 0.247108 tig0026572:-31479 None:intergenic
GGAAGTGTGACATCACTTTC+AGG 0.249099 tig0026572:+33459 Msa1429840:CDS
TAAAATACTCTGCAGTTTCT+TGG 0.255279 tig0026572:-31626 None:intergenic
TCAAAGGTCTCATTCCATTT+CGG 0.277608 tig0026572:-30967 None:intergenic
GGGTTGATTCCCTATCATTC+TGG 0.280304 tig0026572:-30938 None:intergenic
TTAATGTTCTTGTGCTTGTT+TGG 0.292615 tig0026572:+31547 Msa1429840:CDS
ACAAACGGGAACTATTTCTT+TGG 0.299627 tig0026572:-30193 None:intergenic
TCAACCCATCTGTCAATTAT+AGG 0.308791 tig0026572:-31105 None:intergenic
AAAGTCTTTATTTACATAAC+TGG 0.310682 tig0026572:+31420 Msa1429840:CDS
ATACAGAAATAACCTTGATT+AGG 0.319930 tig0026572:+31454 Msa1429840:CDS
ATTCTATCAAGCTCTTGAAT+TGG 0.328163 tig0026572:-32068 None:intergenic
CATCAACAACTATAGTCTTT+TGG 0.330540 tig0026572:-31746 None:intergenic
AAGAACATTAACACCTTCAT+TGG 0.337331 tig0026572:-31535 None:intergenic
CCTAATTCAATAACTTCATT+TGG 0.339645 tig0026572:-32200 None:intergenic
TACAGAAATAACCTTGATTA+GGG 0.344363 tig0026572:+31455 Msa1429840:CDS
CTCATTCCATTTCGGGTTTG+AGG 0.344995 tig0026572:-30959 None:intergenic
AGAATCAAAGCCATTGTATC+AGG 0.349603 tig0026572:-33495 None:intergenic
CTTCAACAGGATACCGTAAA+AGG 0.350159 tig0026572:-33425 None:intergenic
CGAACAGTTATCAGTTTCAT+AGG 0.359772 tig0026572:+31795 Msa1429840:CDS
GATATTAAAGTTGAGGATAT+TGG 0.365230 tig0026572:+32359 Msa1429840:CDS
CATCCATGGATACCAATATC+TGG 0.368621 tig0026572:+31336 Msa1429840:CDS
TTCTATCAAGCTCTTGAATT+GGG 0.378843 tig0026572:-32067 None:intergenic
CCAATTGGTGCAACTCTAAT+CGG 0.382314 tig0026572:+31045 Msa1429840:CDS
AGACCACAAGGTCAAGTATA+TGG 0.383000 tig0026572:+33246 Msa1429840:CDS
GTTGGACGAACCAGAATGAT+AGG 0.386126 tig0026572:+30928 Msa1429840:CDS
CATCTTACCTATCATCATCT+TGG 0.386141 tig0026572:-32741 None:intergenic
ACCTGAGAGTGCATCAGTTC+AGG 0.390624 tig0026572:+32490 Msa1429840:CDS
CATGATTTCATCAAAGTGTA+AGG 0.391165 tig0026572:-33664 None:intergenic
CTGTCAGGCTCTGGTTGCTC+TGG 0.392079 tig0026572:-32668 None:intergenic
CCATCTTCACCCACTTCAAC+AGG 0.395226 tig0026572:-33438 None:intergenic
CCGATTAGAGTTGCACCAAT+TGG 0.395417 tig0026572:-31045 None:intergenic
TAATGTTCTTGTGCTTGTTT+GGG 0.399896 tig0026572:+31548 Msa1429840:CDS
ATATCTTCCCAACATCTTCC+AGG 0.400559 tig0026572:-31375 None:intergenic
ATTGAAAATCAGTATTTCCT+AGG 0.407400 tig0026572:+32311 Msa1429840:CDS
CAGTTCAGGCAATATTAGAT+TGG 0.408091 tig0026572:+32504 Msa1429840:CDS
ACCGTGTTGCTGAGAGGAAT+TGG 0.410354 tig0026572:+33358 Msa1429840:CDS
ATCAACCCTCAAACCCGAAA+TGG 0.413276 tig0026572:+30953 Msa1429840:CDS
CAGAAGAAGATGGCGAAACT+TGG 0.417133 tig0026572:+32120 Msa1429840:CDS
CAAAAGACTATAGTTGTTGA+TGG 0.423538 tig0026572:+31747 Msa1429840:CDS
CTTGTTTGGGTCCCTAATCA+AGG 0.426022 tig0026572:-31466 None:intergenic
GCATTATGGTATGAACATAT+TGG 0.427241 tig0026572:+33279 Msa1429840:CDS
CAAGCTCTTGAATTGGGAAC+AGG 0.427300 tig0026572:-32061 None:intergenic
AAAGACTTTAGCATTCATAA+TGG 0.428519 tig0026572:-31406 None:intergenic
GGAAATAATGGCTGAACAAA+CGG 0.432945 tig0026572:-30208 None:intergenic
ATGGCTTCAGGAGAGCATTA+TGG 0.435411 tig0026572:+33265 Msa1429840:CDS
GATATTCACTGCAAGCTAGA+AGG 0.435535 tig0026572:+31963 Msa1429840:CDS
GTCAAAGATGATAATCCAAT+TGG 0.435782 tig0026572:+31030 Msa1429840:CDS
TCATTCCATTTCGGGTTTGA+GGG 0.436659 tig0026572:-30958 None:intergenic
GTTCATTCCAAGATGATGAT+AGG 0.436783 tig0026572:+32734 Msa1429840:CDS
TAACCATAAGTGAAGTTGAT+AGG 0.438118 tig0026572:+30358 Msa1429840:CDS
CTATGATAACAACCTTGTCT+TGG 0.440091 tig0026572:-33590 None:intergenic
ATCTTCAATGACAACTCCTT+TGG 0.440344 tig0026572:-32398 None:intergenic
ATCCATGGATGAACACTGTC+AGG 0.442733 tig0026572:-31324 None:intergenic
GAATTGGGAACAGGAATTGT+TGG 0.442982 tig0026572:-32052 None:intergenic
CATTGATCTTTGGTTTATGT+TGG 0.443067 tig0026572:-33145 None:intergenic
TCCTAGGGAGTTCCTATGGT+TGG 0.446572 tig0026572:+32327 Msa1429840:CDS
GCTGCTACATGGGACACTTA+AGG 0.452370 tig0026572:+30335 Msa1429840:CDS
ACAAGATACAACCCTTGACT+TGG 0.452543 tig0026572:-30745 None:intergenic
TTTCTTGAGTATTTCTCCTA+GGG 0.452951 tig0026572:-31508 None:intergenic
GCACTCTCAGGTATACCTTC+TGG 0.455321 tig0026572:-32479 None:intergenic
TTGTGGTCTTTGTGAGCCAT+TGG 0.456429 tig0026572:-33232 None:intergenic
CCAGGTGCTTCTATTAAGAA+AGG 0.457313 tig0026572:+31918 Msa1429840:CDS
ATTGTTAGCAAAATTGAAGC+TGG 0.460007 tig0026572:+32419 Msa1429840:CDS
GGACGACAGAACCTCTGTTC+CGG 0.460427 tig0026572:+31569 Msa1429840:CDS
AAGGTCAAGTATATGGCTTC+AGG 0.460768 tig0026572:+33253 Msa1429840:CDS
CAGTTGTTTAGATCCATTGA+TGG 0.464754 tig0026572:+32149 Msa1429840:CDS
GTTCCAGATATTGGTATCCA+TGG 0.465135 tig0026572:-31339 None:intergenic
TAACTTCATTTGGTGCTTGA+GGG 0.468911 tig0026572:-32190 None:intergenic
AAAATGCCTCAGCTGCTACA+TGG 0.472016 tig0026572:+30324 Msa1429840:exon
ATAACTTCATTTGGTGCTTG+AGG 0.473405 tig0026572:-32191 None:intergenic
GAAATAATGGCTGAACAAAC+GGG 0.474517 tig0026572:-30207 None:intergenic
AGAAACAGCACCACCATCAA+TGG 0.474765 tig0026572:-32162 None:intergenic
CCTGTTGAAGTGGGTGAAGA+TGG 0.476067 tig0026572:+33438 Msa1429840:CDS
AAAAGGGAGAGTAAGAAAGA+TGG 0.477240 tig0026572:+32632 Msa1429840:CDS
GCCTGAACTGATGCACTCTC+AGG 0.477938 tig0026572:-32491 None:intergenic
TATTTCCTAGGGAGTTCCTA+TGG 0.478923 tig0026572:+32323 Msa1429840:CDS
TTTGCGATCCTTCAACATGA+TGG 0.480827 tig0026572:-31770 None:intergenic
TGATAATCACTGTCAGGCTC+TGG 0.481330 tig0026572:-32677 None:intergenic
TCTTTGTGAGCCATTGGATG+TGG 0.483037 tig0026572:-33226 None:intergenic
ATTGAATTAGGCCTTGTTAG+TGG 0.484468 tig0026572:+32212 Msa1429840:CDS
GTTGATGAAAATCATCATGA+TGG 0.485239 tig0026572:-31887 None:intergenic
GATATAGATCATAGCATAGC+AGG 0.485628 tig0026572:+30275 Msa1429840:five_prime_UTR
GAAGATCATCGTCCTGTACC+AGG 0.485983 tig0026572:+31141 Msa1429840:CDS
GATTTAGATAAAGCAAGGGT+TGG 0.486390 tig0026572:+30910 Msa1429840:CDS
CAATATTAGATTGGCAAAGA+AGG 0.486949 tig0026572:+32513 Msa1429840:CDS
TCTTTATTTACATAACTGGT+TGG 0.487503 tig0026572:+31424 Msa1429840:CDS
TCAGGCTCTGGTTGCTCTGG+AGG 0.487804 tig0026572:-32665 None:intergenic
CACTTGTGATGAAGATTGTT+GGG 0.493040 tig0026572:+30866 Msa1429840:intron
TACAACACATCCCATGCAAT+AGG 0.493852 tig0026572:-31987 None:intergenic
AGAGGTGGGAAAAGCAAGTC+GGG 0.494783 tig0026572:+32021 Msa1429840:CDS
AGGGTGATACTACTTTCTGT+TGG 0.495579 tig0026572:-31489 None:intergenic
CCACTTGTGATGAAGATTGT+TGG 0.497598 tig0026572:+30865 Msa1429840:intron
TTATGTACCTGGAAGATGTT+GGG 0.499994 tig0026572:+31368 Msa1429840:CDS
TCTTCGTATAGCATTTATGT+AGG 0.504718 tig0026572:-32270 None:intergenic
GCTCTATGATAATCACTGTC+AGG 0.504896 tig0026572:-32683 None:intergenic
ATATCATGCCACGGTTCTCT+AGG 0.506247 tig0026572:-31945 None:intergenic
AGGTGGTAAGTATGGGAGGA+AGG 0.511866 tig0026572:-33536 None:intergenic
CCTTTCTTAATAGAAGCACC+TGG 0.512394 tig0026572:-31918 None:intergenic
TTTACGGTATCCTGTTGAAG+TGG 0.514342 tig0026572:+33428 Msa1429840:CDS
AAACCGTCTTTCTTAAAAGC+CGG 0.515049 tig0026572:-31588 None:intergenic
TTCAACTGATATTAAAGTTG+AGG 0.520279 tig0026572:+32352 Msa1429840:CDS
TTGAAAATCAGTATTTCCTA+GGG 0.521701 tig0026572:+32312 Msa1429840:CDS
TAATTTGGATTTCTGTCGAA+AGG 0.523952 tig0026572:+30395 Msa1429840:CDS
AAATGCCTCAGCTGCTACAT+GGG 0.524555 tig0026572:+30325 Msa1429840:exon
AGAGAGGTTTGTTGTGTATG+TGG 0.525642 tig0026572:+32442 Msa1429840:CDS
AAGCACTGTGCTAGCTAGCT+AGG 0.526978 tig0026572:-33562 None:intergenic
GGGGTCGGGAGAGGAAATAA+TGG 0.528859 tig0026572:-30220 None:intergenic
CAACATAAACCAAAGATCAA+TGG 0.528894 tig0026572:+33146 Msa1429840:CDS
TTTAAGAAAGACGGTTTGAT+GGG 0.532749 tig0026572:+31594 Msa1429840:CDS
TTGAGGATAGAAAATGTCTA+TGG 0.533093 tig0026572:+31205 Msa1429840:CDS
CTAGCTAGCACAGTGCTTGA+CGG 0.534749 tig0026572:+33567 Msa1429840:three_prime_UTR
TGATGAAATCATGTGGTACA+AGG 0.537673 tig0026572:+33674 Msa1429840:three_prime_UTR
AGAAAATGTCTATGGTCTCA+AGG 0.538595 tig0026572:+31213 Msa1429840:CDS
TCTGATAACATTGCATCAGA+AGG 0.542244 tig0026572:-32101 None:intergenic
AGCTAGAAGGACCTATTGCA+TGG 0.543044 tig0026572:+31976 Msa1429840:CDS
TTTCATCAACCAAATTTCCC+AGG 0.543862 tig0026572:+31900 Msa1429840:CDS
GGTATACCTTCTGGCCACAT+TGG 0.545537 tig0026572:-32470 None:intergenic
AAGGACCTATAATTGACAGA+TGG 0.549514 tig0026572:+31100 Msa1429840:CDS
TCAAGGGTTGTATCTTGTGC+CGG 0.551228 tig0026572:+30750 Msa1429840:CDS
GAAGTGTGACATCACTTTCA+GGG 0.551309 tig0026572:+33460 Msa1429840:CDS
TTCGATCAGAGACAAGGTTG+TGG 0.553785 tig0026572:+31273 Msa1429840:CDS
GTTGATGGCCATCATGTTGA+AGG 0.554676 tig0026572:+31762 Msa1429840:CDS
GACTAGGTGGTAAGTATGGG+AGG 0.555923 tig0026572:-33540 None:intergenic
TTGTATCTTGTGCCGGCCTG+AGG 0.558222 tig0026572:+30757 Msa1429840:CDS
AGAGACACAGAGATTGCAAT+GGG 0.562687 tig0026572:+33177 Msa1429840:CDS
ATAACATTGCATCAGAAGGA+TGG 0.563136 tig0026572:-32097 None:intergenic
CAGTTGATTTAGATAAAGCA+AGG 0.564307 tig0026572:+30905 Msa1429840:CDS
ATTATGTACCTGGAAGATGT+TGG 0.564788 tig0026572:+31367 Msa1429840:CDS
AGGACCTATAATTGACAGAT+GGG 0.565090 tig0026572:+31101 Msa1429840:CDS
GATGATGAATACATAGTGAT+TGG 0.566916 tig0026572:+33117 Msa1429840:CDS
TTACGGTATCCTGTTGAAGT+GGG 0.569394 tig0026572:+33429 Msa1429840:CDS
TGTGCTAGCTAGCTAGGACT+AGG 0.570468 tig0026572:-33556 None:intergenic
GCAATTCTCTAGTGTCATTG+AGG 0.571602 tig0026572:+31188 Msa1429840:CDS
GGAACAAAATATTATGTACC+TGG 0.572833 tig0026572:+31357 Msa1429840:CDS
TCCCTGACAGTGTTCATCCA+TGG 0.573348 tig0026572:+31322 Msa1429840:CDS
CAAAATGTAGGGGTCGGGAG+AGG 0.575553 tig0026572:-30229 None:intergenic
GAGATTTCTAGCCCAAGTCA+AGG 0.578002 tig0026572:+30733 Msa1429840:CDS
TAGGACTAGGTGGTAAGTAT+GGG 0.578728 tig0026572:-33543 None:intergenic
GCAAAATTGAAGCTGGAGAG+AGG 0.581821 tig0026572:+32426 Msa1429840:CDS
CTCAGTATACATCATCTCCA+TGG 0.584166 tig0026572:-32537 None:intergenic
TGTCAGGGACATGAGCGTCT+TGG 0.584673 tig0026572:-31308 None:intergenic
CAAGATACAACCCTTGACTT+GGG 0.589678 tig0026572:-30744 None:intergenic
CATCTAGCATCCACATCCAA+TGG 0.592837 tig0026572:+33216 Msa1429840:CDS
TTATGTCCGCGTAACCCTGA+TGG 0.596322 tig0026572:+31672 Msa1429840:CDS
GCCAACCATAGGAACTCCCT+AGG 0.597582 tig0026572:-32328 None:intergenic
TCCAATTCCTCTCAGCAACA+CGG 0.598333 tig0026572:-33359 None:intergenic
GAAGTTGATAGGTTGCAAGC+TGG 0.598778 tig0026572:+30369 Msa1429840:CDS
ATGAGCACAATAGATTTCAA+AGG 0.601968 tig0026572:-30983 None:intergenic
TGCATTGCATCTCATACCAA+AGG 0.606137 tig0026572:+32382 Msa1429840:CDS
AGATTTCTAGCCCAAGTCAA+GGG 0.608267 tig0026572:+30734 Msa1429840:CDS
ATATAGATCATAGCATAGCA+GGG 0.609293 tig0026572:+30276 Msa1429840:five_prime_UTR
TTGTTTAGATCCATTGATGG+TGG 0.610089 tig0026572:+32152 Msa1429840:CDS
ATCAGTTGAATGCCAACCAT+AGG 0.610416 tig0026572:-32339 None:intergenic
TTAAATTGTGTACCTCAGGC+CGG 0.610436 tig0026572:-30769 None:intergenic
AACCAAAGATCAATGGATGG+TGG 0.611885 tig0026572:+33153 Msa1429840:CDS
CAGAGGTGGGAAAAGCAAGT+CGG 0.616576 tig0026572:+32020 Msa1429840:CDS
TTTCCACCATCAGGGTTACG+CGG 0.618021 tig0026572:-31678 None:intergenic
AGTTGATTTAGATAAAGCAA+GGG 0.621560 tig0026572:+30906 Msa1429840:CDS
TACACTTTGATGAAATCATG+TGG 0.621582 tig0026572:+33667 Msa1429840:three_prime_UTR
TTGGACGAACCAGAATGATA+GGG 0.623042 tig0026572:+30929 Msa1429840:CDS
ACAGAAAGTAGTATCACCCT+AGG 0.623457 tig0026572:+31492 Msa1429840:CDS
AGTGTCCCATGTAGCAGCTG+AGG 0.627470 tig0026572:-30330 None:intergenic
GGTGGTATTGATCTGTGTGA+TGG 0.628015 tig0026572:+31816 Msa1429840:CDS
TAAAGGGAACAACACACAAG+GGG 0.628918 tig0026572:+30708 Msa1429840:intron
AGTGCTTGACGGCCAAGACA+AGG 0.628973 tig0026572:+33578 Msa1429840:three_prime_UTR
GTGTTCAAAGTTGAAAACAA+AGG 0.629611 tig0026572:-31861 None:intergenic
GCTAGAAGGACCTATTGCAT+GGG 0.631026 tig0026572:+31977 Msa1429840:CDS
TGTCCGCGTAACCCTGATGG+TGG 0.631372 tig0026572:+31675 Msa1429840:CDS
ACAGTTATCAGTTTCATAGG+TGG 0.633175 tig0026572:+31798 Msa1429840:CDS
ATAAACCAAAGATCAATGGA+TGG 0.633380 tig0026572:+33150 Msa1429840:CDS
CCTTCCGAGAAAATTCTCAA+AGG 0.639521 tig0026572:+31081 Msa1429840:CDS
AAGCCATATACTTGACCTTG+TGG 0.641516 tig0026572:-33249 None:intergenic
TTTCTTAAAAGCCGGAACAG+AGG 0.644428 tig0026572:-31580 None:intergenic
CTTTCTTAATAGAAGCACCT+GGG 0.646815 tig0026572:-31917 None:intergenic
GGTGCTTCTATTAAGAAAGG+TGG 0.653659 tig0026572:+31921 Msa1429840:CDS
GTGATTCCAATGTGGCCAGA+AGG 0.653982 tig0026572:+32464 Msa1429840:CDS
GCAATGTTATCAGAAGAAGA+TGG 0.655613 tig0026572:+32110 Msa1429840:CDS
ATAGTGTATGAACGAGCAAG+TGG 0.657900 tig0026572:-33715 None:intergenic
AAGAGACACAGAGATTGCAA+TGG 0.659014 tig0026572:+33176 Msa1429840:CDS
TTGCAGTGAATATCATGCCA+CGG 0.660624 tig0026572:-31954 None:intergenic
AATAAAGGGAACAACACACA+AGG 0.665452 tig0026572:+30706 Msa1429840:intron
AGATTGGCAAAGAAGGACCA+TGG 0.665799 tig0026572:+32520 Msa1429840:CDS
GCTAGCTAGCTAGGACTAGG+TGG 0.669608 tig0026572:-33553 None:intergenic
TGATGGAAGATATGATACAA+TGG 0.676865 tig0026572:+31833 Msa1429840:CDS
GATGTGGATGCTAGATGATG+AGG 0.676901 tig0026572:-33210 None:intergenic
TAAAATGTCAAATAATCACG+AGG 0.677762 tig0026572:-32599 None:intergenic
AAAATGTCAAATAATCACGA+GGG 0.681608 tig0026572:-32598 None:intergenic
TCCATGGATGAACACTGTCA+GGG 0.688183 tig0026572:-31323 None:intergenic
ATAAAGGGAACAACACACAA+GGG 0.688691 tig0026572:+30707 Msa1429840:intron
AATGGCTCACAAAGACCACA+AGG 0.712737 tig0026572:+33234 Msa1429840:CDS
TGTATGTGGTGATTCCAATG+TGG 0.713699 tig0026572:+32456 Msa1429840:CDS
GATCATCGTCCTGTACCAGG+CGG 0.755483 tig0026572:+31144 Msa1429840:CDS
AAGGTGGTCCTAGAGAACCG+TGG 0.794041 tig0026572:+31937 Msa1429840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATTTTTTACAAAATGTA+GGG - tig0026572:30243-30262 None:intergenic 15.0%
!!! TTCAATTTTTTACAAAATGT+AGG - tig0026572:30244-30263 None:intergenic 15.0%
!! TTGAAGTTTAAATTCATAAA+TGG + tig0026572:30480-30499 Msa1429840:intron 15.0%
!! CATATAAGCTATTATATTAT+AGG - tig0026572:32776-32795 None:intergenic 15.0%
!! AAAAGATTTATTTGAATGTT+TGG - tig0026572:32813-32832 None:intergenic 15.0%
!!! ACATTCAAATAAATCTTTTT+AGG + tig0026572:32814-32833 Msa1429840:intron 15.0%
!! AAACCTAGAAAATATATATT+TGG + tig0026572:33008-33027 Msa1429840:intron 15.0%
!!! CAATTTTTTACAAAATGTAG+GGG - tig0026572:30242-30261 None:intergenic 20.0%
!! CACTTTAATTATTAAAACAC+TGG - tig0026572:30441-30460 None:intergenic 20.0%
!! AAATGGCAAAATATATTATG+CGG + tig0026572:30497-30516 Msa1429840:intron 20.0%
!! AATTTGTGTATTCAATGAAT+TGG + tig0026572:30587-30606 Msa1429840:intron 20.0%
!!! TAATATTTTGTTCCAGATAT+TGG - tig0026572:31351-31370 None:intergenic 20.0%
!!! AAAGTCTTTATTTACATAAC+TGG + tig0026572:31420-31439 Msa1429840:CDS 20.0%
!!! AGAGTATTTTAAAAACACAA+AGG + tig0026572:31638-31657 Msa1429840:CDS 20.0%
!!! TTCAACATTCTTCTTTTTTA+TGG - tig0026572:32573-32592 None:intergenic 20.0%
!!! TATTTGACATTTTATTGTCT+TGG + tig0026572:32608-32627 Msa1429840:CDS 20.0%
!!! ATTTTATTGTCTTGGTAAAA+GGG + tig0026572:32616-32635 Msa1429840:CDS 20.0%
!!! TTCAAATAAATCTTTTTAGG+TGG + tig0026572:32817-32836 Msa1429840:intron 20.0%
!! AATGAAAAATCATGATAGTT+AGG - tig0026572:32973-32992 None:intergenic 20.0%
!!! ATTTTCTAGGTTTTGAATAT+AGG - tig0026572:33001-33020 None:intergenic 20.0%
!!! ATGCCAAATATATATTTTCT+AGG - tig0026572:33014-33033 None:intergenic 20.0%
!! ATAAAGTAAACAATCAATGT+TGG - tig0026572:33082-33101 None:intergenic 20.0%
! CAAAATATATTATGCGGTAT+TGG + tig0026572:30503-30522 Msa1429840:intron 25.0%
! AAGAAAAAAATATAGTGCCA+CGG - tig0026572:30670-30689 None:intergenic 25.0%
!! TTTTCTTCTGTCAAATATTG+TGG + tig0026572:30683-30702 Msa1429840:intron 25.0%
! TGTCAAATATTGTGGAATAA+AGG + tig0026572:30691-30710 Msa1429840:intron 25.0%
! GTCAAATATTGTGGAATAAA+GGG + tig0026572:30692-30711 Msa1429840:intron 25.0%
! TATACAAATGGTAGAAAGTT+TGG - tig0026572:30795-30814 None:intergenic 25.0%
!! AGTTGATTTAGATAAAGCAA+GGG + tig0026572:30906-30925 Msa1429840:CDS 25.0%
! AAAGACTTTAGCATTCATAA+TGG - tig0026572:31409-31428 None:intergenic 25.0%
!! TCTTTATTTACATAACTGGT+TGG + tig0026572:31424-31443 Msa1429840:CDS 25.0%
! ATACAGAAATAACCTTGATT+AGG + tig0026572:31454-31473 Msa1429840:CDS 25.0%
! TACAGAAATAACCTTGATTA+GGG + tig0026572:31455-31474 Msa1429840:CDS 25.0%
! CCTAATTCAATAACTTCATT+TGG - tig0026572:32203-32222 None:intergenic 25.0%
! CCAAATGAAGTTATTGAATT+AGG + tig0026572:32200-32219 Msa1429840:CDS 25.0%
! ATTGAAAATCAGTATTTCCT+AGG + tig0026572:32311-32330 Msa1429840:CDS 25.0%
! TTGAAAATCAGTATTTCCTA+GGG + tig0026572:32312-32331 Msa1429840:CDS 25.0%
! TTCAACTGATATTAAAGTTG+AGG + tig0026572:32352-32371 Msa1429840:CDS 25.0%
! GATATTAAAGTTGAGGATAT+TGG + tig0026572:32359-32378 Msa1429840:CDS 25.0%
! AAAATGTCAAATAATCACGA+GGG - tig0026572:32601-32620 None:intergenic 25.0%
! TAAAATGTCAAATAATCACG+AGG - tig0026572:32602-32621 None:intergenic 25.0%
!! CATTTTATTGTCTTGGTAAA+AGG + tig0026572:32615-32634 Msa1429840:CDS 25.0%
!! GTGTTTCATATGGTTTTTAT+TGG + tig0026572:32856-32875 Msa1429840:intron 25.0%
! AAAAATCATGATAGTTAGGT+AGG - tig0026572:32969-32988 None:intergenic 25.0%
! AAAAATCTATGAAACAAGTG+AGG - tig0026572:33743-33762 None:intergenic 25.0%
! TTTTTACAAAATGTAGGGGT+CGG - tig0026572:30238-30257 None:intergenic 30.0%
ATATAGATCATAGCATAGCA+GGG + tig0026572:30276-30295 Msa1429840:five_prime_UTR 30.0%
! TAACCATAAGTGAAGTTGAT+AGG + tig0026572:30358-30377 Msa1429840:CDS 30.0%
TAATTTGGATTTCTGTCGAA+AGG + tig0026572:30395-30414 Msa1429840:CDS 30.0%
!! AAGTCTTTAAAAAGTGTCAC+AGG - tig0026572:30547-30566 None:intergenic 30.0%
! AGCTCATTTAATTTAATCCG+TGG + tig0026572:30650-30669 Msa1429840:intron 30.0%
! ACGTGAAAAGCATATACAAA+TGG - tig0026572:30807-30826 None:intergenic 30.0%
! CAGTTGATTTAGATAAAGCA+AGG + tig0026572:30905-30924 Msa1429840:CDS 30.0%
ATGAGCACAATAGATTTCAA+AGG - tig0026572:30986-31005 None:intergenic 30.0%
GTCAAAGATGATAATCCAAT+TGG + tig0026572:31030-31049 Msa1429840:CDS 30.0%
TTGAGGATAGAAAATGTCTA+TGG + tig0026572:31205-31224 Msa1429840:CDS 30.0%
GGAACAAAATATTATGTACC+TGG + tig0026572:31357-31376 Msa1429840:CDS 30.0%
TTTCTTGAGTATTTCTCCTA+GGG - tig0026572:31511-31530 None:intergenic 30.0%
! TTTTCTTGAGTATTTCTCCT+AGG - tig0026572:31512-31531 None:intergenic 30.0%
AAGAACATTAACACCTTCAT+TGG - tig0026572:31538-31557 None:intergenic 30.0%
TTAATGTTCTTGTGCTTGTT+TGG + tig0026572:31547-31566 Msa1429840:CDS 30.0%
TAATGTTCTTGTGCTTGTTT+GGG + tig0026572:31548-31567 Msa1429840:CDS 30.0%
!! TTTTAAGAAAGACGGTTTGA+TGG + tig0026572:31593-31612 Msa1429840:CDS 30.0%
TTTAAGAAAGACGGTTTGAT+GGG + tig0026572:31594-31613 Msa1429840:CDS 30.0%
TAAAATACTCTGCAGTTTCT+TGG - tig0026572:31629-31648 None:intergenic 30.0%
! CATCAACAACTATAGTCTTT+TGG - tig0026572:31749-31768 None:intergenic 30.0%
CAAAAGACTATAGTTGTTGA+TGG + tig0026572:31747-31766 Msa1429840:CDS 30.0%
TGATGGAAGATATGATACAA+TGG + tig0026572:31833-31852 Msa1429840:CDS 30.0%
GTGTTCAAAGTTGAAAACAA+AGG - tig0026572:31864-31883 None:intergenic 30.0%
! GTTGATGAAAATCATCATGA+TGG - tig0026572:31890-31909 None:intergenic 30.0%
TTCTATCAAGCTCTTGAATT+GGG - tig0026572:32070-32089 None:intergenic 30.0%
ATTCTATCAAGCTCTTGAAT+TGG - tig0026572:32071-32090 None:intergenic 30.0%
TCTTCGTATAGCATTTATGT+AGG - tig0026572:32273-32292 None:intergenic 30.0%
ATTGTTAGCAAAATTGAAGC+TGG + tig0026572:32419-32438 Msa1429840:CDS 30.0%
CAATATTAGATTGGCAAAGA+AGG + tig0026572:32513-32532 Msa1429840:CDS 30.0%
TGAATGTTTGGACTTTGTAA+AGG - tig0026572:32801-32820 None:intergenic 30.0%
!!! ATCTTTTTAGGTGGTTTCTA+AGG + tig0026572:32826-32845 Msa1429840:intron 30.0%
GAAACTCTAACAACTATCAA+TGG + tig0026572:32901-32920 Msa1429840:intron 30.0%
ATTTAGTCCAATCTTCTTAC+GGG - tig0026572:32932-32951 None:intergenic 30.0%
CATTTAGTCCAATCTTCTTA+CGG - tig0026572:32933-32952 None:intergenic 30.0%
!!! ACTTTATTTTGTTCCTGTAG+TGG + tig0026572:33095-33114 Msa1429840:intron 30.0%
GATGATGAATACATAGTGAT+TGG + tig0026572:33117-33136 Msa1429840:CDS 30.0%
! CATTGATCTTTGGTTTATGT+TGG - tig0026572:33148-33167 None:intergenic 30.0%
CAACATAAACCAAAGATCAA+TGG + tig0026572:33146-33165 Msa1429840:CDS 30.0%
ATAAACCAAAGATCAATGGA+TGG + tig0026572:33150-33169 Msa1429840:CDS 30.0%
GCATTATGGTATGAACATAT+TGG + tig0026572:33279-33298 Msa1429840:CDS 30.0%
! GTGTAAATACTCACCTTTTA+CGG + tig0026572:33412-33431 Msa1429840:CDS 30.0%
! GCAATATTTTCCTGATACAA+TGG + tig0026572:33485-33504 Msa1429840:CDS 30.0%
CATGATTTCATCAAAGTGTA+AGG - tig0026572:33667-33686 None:intergenic 30.0%
TACACTTTGATGAAATCATG+TGG + tig0026572:33667-33686 Msa1429840:three_prime_UTR 30.0%
GAAATAATGGCTGAACAAAC+GGG - tig0026572:30210-30229 None:intergenic 35.0%
GGAAATAATGGCTGAACAAA+CGG - tig0026572:30211-30230 None:intergenic 35.0%
! TTTTACAAAATGTAGGGGTC+GGG - tig0026572:30237-30256 None:intergenic 35.0%
GATATAGATCATAGCATAGC+AGG + tig0026572:30275-30294 Msa1429840:five_prime_UTR 35.0%
CAACCTATCAACTTCACTTA+TGG - tig0026572:30364-30383 None:intergenic 35.0%
AATAAAGGGAACAACACACA+AGG + tig0026572:30706-30725 Msa1429840:intron 35.0%
ATAAAGGGAACAACACACAA+GGG + tig0026572:30707-30726 Msa1429840:intron 35.0%
TTGGTTAAATTGTGTACCTC+AGG - tig0026572:30776-30795 None:intergenic 35.0%
CACTTGTGATGAAGATTGTT+GGG + tig0026572:30866-30885 Msa1429840:intron 35.0%
GATTTAGATAAAGCAAGGGT+TGG + tig0026572:30910-30929 Msa1429840:CDS 35.0%
TCAAAGGTCTCATTCCATTT+CGG - tig0026572:30970-30989 None:intergenic 35.0%
!! CTTTGAGAATTTTCTCGGAA+GGG - tig0026572:31083-31102 None:intergenic 35.0%
!! AGGTCCTTTGAGAATTTTCT+CGG - tig0026572:31088-31107 None:intergenic 35.0%
AAGGACCTATAATTGACAGA+TGG + tig0026572:31100-31119 Msa1429840:CDS 35.0%
AGGACCTATAATTGACAGAT+GGG + tig0026572:31101-31120 Msa1429840:CDS 35.0%
TCAACCCATCTGTCAATTAT+AGG - tig0026572:31108-31127 None:intergenic 35.0%
AGAAAATGTCTATGGTCTCA+AGG + tig0026572:31213-31232 Msa1429840:CDS 35.0%
!!! AGGAATACACATGCCATTTT+TGG + tig0026572:31233-31252 Msa1429840:CDS 35.0%
!!! GGAATACACATGCCATTTTT+GGG + tig0026572:31234-31253 Msa1429840:CDS 35.0%
!! ACATTTTTCGATCAGAGACA+AGG + tig0026572:31267-31286 Msa1429840:CDS 35.0%
ATTATGTACCTGGAAGATGT+TGG + tig0026572:31367-31386 Msa1429840:CDS 35.0%
TTATGTACCTGGAAGATGTT+GGG + tig0026572:31368-31387 Msa1429840:CDS 35.0%
!! TACTTTCTGTTGGCTTGTTT+GGG - tig0026572:31482-31501 None:intergenic 35.0%
CTCAAGAAAAAAGCCAATGA+AGG + tig0026572:31522-31541 Msa1429840:CDS 35.0%
AAACCGTCTTTCTTAAAAGC+CGG - tig0026572:31591-31610 None:intergenic 35.0%
CGAACAGTTATCAGTTTCAT+AGG + tig0026572:31795-31814 Msa1429840:CDS 35.0%
ACAGTTATCAGTTTCATAGG+TGG + tig0026572:31798-31817 Msa1429840:CDS 35.0%
TTTCATCAACCAAATTTCCC+AGG + tig0026572:31900-31919 Msa1429840:CDS 35.0%
!! CTTTCTTAATAGAAGCACCT+GGG - tig0026572:31920-31939 None:intergenic 35.0%
! TGTTGTACAATTTTGAGCAG+AGG + tig0026572:32003-32022 Msa1429840:CDS 35.0%
ATAACATTGCATCAGAAGGA+TGG - tig0026572:32100-32119 None:intergenic 35.0%
TCTGATAACATTGCATCAGA+AGG - tig0026572:32104-32123 None:intergenic 35.0%
GCAATGTTATCAGAAGAAGA+TGG + tig0026572:32110-32129 Msa1429840:CDS 35.0%
CAGTTGTTTAGATCCATTGA+TGG + tig0026572:32149-32168 Msa1429840:CDS 35.0%
TTGTTTAGATCCATTGATGG+TGG + tig0026572:32152-32171 Msa1429840:CDS 35.0%
!! TAACTTCATTTGGTGCTTGA+GGG - tig0026572:32193-32212 None:intergenic 35.0%
!! ATAACTTCATTTGGTGCTTG+AGG - tig0026572:32194-32213 None:intergenic 35.0%
!! ATTGAATTAGGCCTTGTTAG+TGG + tig0026572:32212-32231 Msa1429840:CDS 35.0%
! TGTTGTCTTTTCCACTAACA+AGG - tig0026572:32226-32245 None:intergenic 35.0%
ATCTTCAATGACAACTCCTT+TGG - tig0026572:32401-32420 None:intergenic 35.0%
CAGTTCAGGCAATATTAGAT+TGG + tig0026572:32504-32523 Msa1429840:CDS 35.0%
AAAAGGGAGAGTAAGAAAGA+TGG + tig0026572:32632-32651 Msa1429840:CDS 35.0%
GTTCATTCCAAGATGATGAT+AGG + tig0026572:32734-32753 Msa1429840:CDS 35.0%
CATCTTACCTATCATCATCT+TGG - tig0026572:32744-32763 None:intergenic 35.0%
!!! TTTTTAGGTGGTTTCTAAGG+TGG + tig0026572:32829-32848 Msa1429840:intron 35.0%
!!! TTTTAGGTGGTTTCTAAGGT+GGG + tig0026572:32830-32849 Msa1429840:intron 35.0%
ATGTATTCATCATCCACTAC+AGG - tig0026572:33111-33130 None:intergenic 35.0%
AGAATCAAAGCCATTGTATC+AGG - tig0026572:33498-33517 None:intergenic 35.0%
!! GATACAATGGCTTTGATTCT+TGG + tig0026572:33498-33517 Msa1429840:CDS 35.0%
CTATGATAACAACCTTGTCT+TGG - tig0026572:33593-33612 None:intergenic 35.0%
TGATGAAATCATGTGGTACA+AGG + tig0026572:33674-33693 Msa1429840:three_prime_UTR 35.0%
GTTGCAAGCTGGATGTAATT+TGG + tig0026572:30380-30399 Msa1429840:CDS 40.0%
TAAAGGGAACAACACACAAG+GGG + tig0026572:30708-30727 Msa1429840:intron 40.0%
!! AGATTTCTAGCCCAAGTCAA+GGG + tig0026572:30734-30753 Msa1429840:CDS 40.0%
CAAGATACAACCCTTGACTT+GGG - tig0026572:30747-30766 None:intergenic 40.0%
ACAAGATACAACCCTTGACT+TGG - tig0026572:30748-30767 None:intergenic 40.0%
TTAAATTGTGTACCTCAGGC+CGG - tig0026572:30772-30791 None:intergenic 40.0%
CCAACAATCTTCATCACAAG+TGG - tig0026572:30868-30887 None:intergenic 40.0%
CCACTTGTGATGAAGATTGT+TGG + tig0026572:30865-30884 Msa1429840:intron 40.0%
TTGGACGAACCAGAATGATA+GGG + tig0026572:30929-30948 Msa1429840:CDS 40.0%
!! TCATTCCATTTCGGGTTTGA+GGG - tig0026572:30961-30980 None:intergenic 40.0%
CAAAGGTCTCATTCCATTTC+GGG - tig0026572:30969-30988 None:intergenic 40.0%
CCAATTGGTGCAACTCTAAT+CGG + tig0026572:31045-31064 Msa1429840:CDS 40.0%
!! CCTTTGAGAATTTTCTCGGA+AGG - tig0026572:31084-31103 None:intergenic 40.0%
CCTTCCGAGAAAATTCTCAA+AGG + tig0026572:31081-31100 Msa1429840:CDS 40.0%
! GCAATTCTCTAGTGTCATTG+AGG + tig0026572:31188-31207 Msa1429840:CDS 40.0%
CATCCATGGATACCAATATC+TGG + tig0026572:31336-31355 Msa1429840:CDS 40.0%
GTTCCAGATATTGGTATCCA+TGG - tig0026572:31342-31361 None:intergenic 40.0%
ATATCTTCCCAACATCTTCC+AGG - tig0026572:31378-31397 None:intergenic 40.0%
!! CTACTTTCTGTTGGCTTGTT+TGG - tig0026572:31483-31502 None:intergenic 40.0%
AGGGTGATACTACTTTCTGT+TGG - tig0026572:31492-31511 None:intergenic 40.0%
ACAGAAAGTAGTATCACCCT+AGG + tig0026572:31492-31511 Msa1429840:CDS 40.0%
TTTCTTAAAAGCCGGAACAG+AGG - tig0026572:31583-31602 None:intergenic 40.0%
!! GTTCCGGCTTTTAAGAAAGA+CGG + tig0026572:31585-31604 Msa1429840:CDS 40.0%
!!! CTATGCTTTTTCCACCATCA+GGG - tig0026572:31689-31708 None:intergenic 40.0%
!!! ACTATGCTTTTTCCACCATC+AGG - tig0026572:31690-31709 None:intergenic 40.0%
! GGTGGAAAAAGCATAGTTCA+AGG + tig0026572:31693-31712 Msa1429840:CDS 40.0%
TTTGCGATCCTTCAACATGA+TGG - tig0026572:31773-31792 None:intergenic 40.0%
!! ATAGAAGCACCTGGGAAATT+TGG - tig0026572:31912-31931 None:intergenic 40.0%
!! CCTTTCTTAATAGAAGCACC+TGG - tig0026572:31921-31940 None:intergenic 40.0%
CCAGGTGCTTCTATTAAGAA+AGG + tig0026572:31918-31937 Msa1429840:CDS 40.0%
GGTGCTTCTATTAAGAAAGG+TGG + tig0026572:31921-31940 Msa1429840:CDS 40.0%
TTGCAGTGAATATCATGCCA+CGG - tig0026572:31957-31976 None:intergenic 40.0%
GATATTCACTGCAAGCTAGA+AGG + tig0026572:31963-31982 Msa1429840:CDS 40.0%
TACAACACATCCCATGCAAT+AGG - tig0026572:31990-32009 None:intergenic 40.0%
! TGTACAATTTTGAGCAGAGG+TGG + tig0026572:32006-32025 Msa1429840:CDS 40.0%
! GTACAATTTTGAGCAGAGGT+GGG + tig0026572:32007-32026 Msa1429840:CDS 40.0%
GAATTGGGAACAGGAATTGT+TGG - tig0026572:32055-32074 None:intergenic 40.0%
TATTTCCTAGGGAGTTCCTA+TGG + tig0026572:32323-32342 Msa1429840:CDS 40.0%
ATCAGTTGAATGCCAACCAT+AGG - tig0026572:32342-32361 None:intergenic 40.0%
TGCATTGCATCTCATACCAA+AGG + tig0026572:32382-32401 Msa1429840:CDS 40.0%
!! AGAGAGGTTTGTTGTGTATG+TGG + tig0026572:32442-32461 Msa1429840:CDS 40.0%
TGTATGTGGTGATTCCAATG+TGG + tig0026572:32456-32475 Msa1429840:CDS 40.0%
CTCAGTATACATCATCTCCA+TGG - tig0026572:32540-32559 None:intergenic 40.0%
GCTCTATGATAATCACTGTC+AGG - tig0026572:32686-32705 None:intergenic 40.0%
!! TTTAGGTGGTTTCTAAGGTG+GGG + tig0026572:32831-32850 Msa1429840:intron 40.0%
AACCAAAGATCAATGGATGG+TGG + tig0026572:33153-33172 Msa1429840:CDS 40.0%
TTCCACCATCCATTGATCTT+TGG - tig0026572:33158-33177 None:intergenic 40.0%
AAGAGACACAGAGATTGCAA+TGG + tig0026572:33176-33195 Msa1429840:CDS 40.0%
AGAGACACAGAGATTGCAAT+GGG + tig0026572:33177-33196 Msa1429840:CDS 40.0%
AGACCACAAGGTCAAGTATA+TGG + tig0026572:33246-33265 Msa1429840:CDS 40.0%
AAGCCATATACTTGACCTTG+TGG - tig0026572:33252-33271 None:intergenic 40.0%
AAGGTCAAGTATATGGCTTC+AGG + tig0026572:33253-33272 Msa1429840:CDS 40.0%
TTTACGCATTCCACACTTTC+AGG - tig0026572:33330-33349 None:intergenic 40.0%
CTTCAACAGGATACCGTAAA+AGG - tig0026572:33428-33447 None:intergenic 40.0%
TTTACGGTATCCTGTTGAAG+TGG + tig0026572:33428-33447 Msa1429840:CDS 40.0%
TTACGGTATCCTGTTGAAGT+GGG + tig0026572:33429-33448 Msa1429840:CDS 40.0%
GAAGTGTGACATCACTTTCA+GGG + tig0026572:33460-33479 Msa1429840:CDS 40.0%
TAGGACTAGGTGGTAAGTAT+GGG - tig0026572:33546-33565 None:intergenic 40.0%
ATAGTGTATGAACGAGCAAG+TGG - tig0026572:33718-33737 None:intergenic 40.0%
AAAATGCCTCAGCTGCTACA+TGG + tig0026572:30324-30343 Msa1429840:exon 45.0%
AAATGCCTCAGCTGCTACAT+GGG + tig0026572:30325-30344 Msa1429840:exon 45.0%
!! GAAGTTGATAGGTTGCAAGC+TGG + tig0026572:30369-30388 Msa1429840:CDS 45.0%
!! GAGATTTCTAGCCCAAGTCA+AGG + tig0026572:30733-30752 Msa1429840:CDS 45.0%
TCAAGGGTTGTATCTTGTGC+CGG + tig0026572:30750-30769 Msa1429840:CDS 45.0%
GTTGGACGAACCAGAATGAT+AGG + tig0026572:30928-30947 Msa1429840:CDS 45.0%
! GGGTTGATTCCCTATCATTC+TGG - tig0026572:30941-30960 None:intergenic 45.0%
ATCAACCCTCAAACCCGAAA+TGG + tig0026572:30953-30972 Msa1429840:CDS 45.0%
!! CTCATTCCATTTCGGGTTTG+AGG - tig0026572:30962-30981 None:intergenic 45.0%
CCGATTAGAGTTGCACCAAT+TGG - tig0026572:31048-31067 None:intergenic 45.0%
!!! ATTTTAGAACCGCCTGGTAC+AGG - tig0026572:31156-31175 None:intergenic 45.0%
!!! ACACGAATTTTAGAACCGCC+TGG - tig0026572:31162-31181 None:intergenic 45.0%
GTACGCTGAACTCCCAAAAA+TGG - tig0026572:31249-31268 None:intergenic 45.0%
TTCGATCAGAGACAAGGTTG+TGG + tig0026572:31273-31292 Msa1429840:CDS 45.0%
TCCATGGATGAACACTGTCA+GGG - tig0026572:31326-31345 None:intergenic 45.0%
ATCCATGGATGAACACTGTC+AGG - tig0026572:31327-31346 None:intergenic 45.0%
CTTGTTTGGGTCCCTAATCA+AGG - tig0026572:31469-31488 None:intergenic 45.0%
!! GTTGATGGCCATCATGTTGA+AGG + tig0026572:31762-31781 Msa1429840:CDS 45.0%
!! GGTGGTATTGATCTGTGTGA+TGG + tig0026572:31816-31835 Msa1429840:CDS 45.0%
ATATCATGCCACGGTTCTCT+AGG - tig0026572:31948-31967 None:intergenic 45.0%
AGCTAGAAGGACCTATTGCA+TGG + tig0026572:31976-31995 Msa1429840:CDS 45.0%
GCTAGAAGGACCTATTGCAT+GGG + tig0026572:31977-31996 Msa1429840:CDS 45.0%
CAAGCTCTTGAATTGGGAAC+AGG - tig0026572:32064-32083 None:intergenic 45.0%
CAGAAGAAGATGGCGAAACT+TGG + tig0026572:32120-32139 Msa1429840:CDS 45.0%
AGAAACAGCACCACCATCAA+TGG - tig0026572:32165-32184 None:intergenic 45.0%
GCAAAATTGAAGCTGGAGAG+AGG + tig0026572:32426-32445 Msa1429840:CDS 45.0%
AGATTGGCAAAGAAGGACCA+TGG + tig0026572:32520-32539 Msa1429840:CDS 45.0%
TGATAATCACTGTCAGGCTC+TGG - tig0026572:32680-32699 None:intergenic 45.0%
!! TTAGGTGGTTTCTAAGGTGG+GGG + tig0026572:32832-32851 Msa1429840:intron 45.0%
GGCATTTCCCGTAAGAAGAT+TGG + tig0026572:32922-32941 Msa1429840:intron 45.0%
GATGTGGATGCTAGATGATG+AGG - tig0026572:33213-33232 None:intergenic 45.0%
CATCTAGCATCCACATCCAA+TGG + tig0026572:33216-33235 Msa1429840:CDS 45.0%
TCTTTGTGAGCCATTGGATG+TGG - tig0026572:33229-33248 None:intergenic 45.0%
TTGTGGTCTTTGTGAGCCAT+TGG - tig0026572:33235-33254 None:intergenic 45.0%
AATGGCTCACAAAGACCACA+AGG + tig0026572:33234-33253 Msa1429840:CDS 45.0%
!! ATGGCTTCAGGAGAGCATTA+TGG + tig0026572:33265-33284 Msa1429840:CDS 45.0%
! TTTTGACGAGCCTGAAAGTG+TGG + tig0026572:33317-33336 Msa1429840:CDS 45.0%
!! TTTTGAACCGTGTTGCTGAG+AGG + tig0026572:33352-33371 Msa1429840:CDS 45.0%
TCCAATTCCTCTCAGCAACA+CGG - tig0026572:33362-33381 None:intergenic 45.0%
! GGAAGTGTGACATCACTTTC+AGG + tig0026572:33459-33478 Msa1429840:CDS 45.0%
CTAGGACTAGGTGGTAAGTA+TGG - tig0026572:33547-33566 None:intergenic 45.0%
!!! AAAAAATTGAATTTTAATTT+AGG + tig0026572:30253-30272 Msa1429840:five_prime_UTR 5.0%
GCTGCTACATGGGACACTTA+AGG + tig0026572:30335-30354 Msa1429840:CDS 50.0%
GAAGATCATCGTCCTGTACC+AGG + tig0026572:31141-31160 Msa1429840:CDS 50.0%
TCCCTGACAGTGTTCATCCA+TGG + tig0026572:31322-31341 Msa1429840:CDS 50.0%
TTATGTCCGCGTAACCCTGA+TGG + tig0026572:31672-31691 Msa1429840:CDS 50.0%
TTTCCACCATCAGGGTTACG+CGG - tig0026572:31681-31700 None:intergenic 50.0%
CAGAGGTGGGAAAAGCAAGT+CGG + tig0026572:32020-32039 Msa1429840:CDS 50.0%
AGAGGTGGGAAAAGCAAGTC+GGG + tig0026572:32021-32040 Msa1429840:CDS 50.0%
TCCTAGGGAGTTCCTATGGT+TGG + tig0026572:32327-32346 Msa1429840:CDS 50.0%
GTGATTCCAATGTGGCCAGA+AGG + tig0026572:32464-32483 Msa1429840:CDS 50.0%
GGTATACCTTCTGGCCACAT+TGG - tig0026572:32473-32492 None:intergenic 50.0%
GCACTCTCAGGTATACCTTC+TGG - tig0026572:32482-32501 None:intergenic 50.0%
! ACCTGAGAGTGCATCAGTTC+AGG + tig0026572:32490-32509 Msa1429840:CDS 50.0%
!! AGGTGGGGGAGTGTTTCATA+TGG + tig0026572:32846-32865 Msa1429840:intron 50.0%
! ACCGTGTTGCTGAGAGGAAT+TGG + tig0026572:33358-33377 Msa1429840:CDS 50.0%
CCATCTTCACCCACTTCAAC+AGG - tig0026572:33441-33460 None:intergenic 50.0%
CCTGTTGAAGTGGGTGAAGA+TGG + tig0026572:33438-33457 Msa1429840:CDS 50.0%
AGGTGGTAAGTATGGGAGGA+AGG - tig0026572:33539-33558 None:intergenic 50.0%
GACTAGGTGGTAAGTATGGG+AGG - tig0026572:33543-33562 None:intergenic 50.0%
TGTGCTAGCTAGCTAGGACT+AGG - tig0026572:33559-33578 None:intergenic 50.0%
! AAGCACTGTGCTAGCTAGCT+AGG - tig0026572:33565-33584 None:intergenic 50.0%
CTAGCTAGCACAGTGCTTGA+CGG + tig0026572:33567-33586 Msa1429840:three_prime_UTR 50.0%
GGGGTCGGGAGAGGAAATAA+TGG - tig0026572:30223-30242 None:intergenic 55.0%
CAAAATGTAGGGGTCGGGAG+AGG - tig0026572:30232-30251 None:intergenic 55.0%
AGTGTCCCATGTAGCAGCTG+AGG - tig0026572:30333-30352 None:intergenic 55.0%
! TTGTATCTTGTGCCGGCCTG+AGG + tig0026572:30757-30776 Msa1429840:CDS 55.0%
GATCATCGTCCTGTACCAGG+CGG + tig0026572:31144-31163 Msa1429840:CDS 55.0%
TGTCAGGGACATGAGCGTCT+TGG - tig0026572:31311-31330 None:intergenic 55.0%
GGACGACAGAACCTCTGTTC+CGG + tig0026572:31569-31588 Msa1429840:CDS 55.0%
AAGGTGGTCCTAGAGAACCG+TGG + tig0026572:31937-31956 Msa1429840:CDS 55.0%
GCCAACCATAGGAACTCCCT+AGG - tig0026572:32331-32350 None:intergenic 55.0%
GCCTGAACTGATGCACTCTC+AGG - tig0026572:32494-32513 None:intergenic 55.0%
GCTAGCTAGCTAGGACTAGG+TGG - tig0026572:33556-33575 None:intergenic 55.0%
AGTGCTTGACGGCCAAGACA+AGG + tig0026572:33578-33597 Msa1429840:three_prime_UTR 55.0%
TGTCCGCGTAACCCTGATGG+TGG + tig0026572:31675-31694 Msa1429840:CDS 60.0%
! TCAGGCTCTGGTTGCTCTGG+AGG - tig0026572:32668-32687 None:intergenic 60.0%
! CTGTCAGGCTCTGGTTGCTC+TGG - tig0026572:32671-32690 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0026572 gene 30194 33780 30194 ID=Msa1429840;Name=Msa1429840
tig0026572 mRNA 30194 33780 30194 ID=Msa1429840-mRNA-1;Parent=Msa1429840;Name=Msa1429840-mRNA-1;_AED=0.01;_eAED=0.01;_QI=133|1|1|1|0.75|0.6|5|188|825
tig0026572 exon 30194 30416 30194 ID=Msa1429840-mRNA-1:exon:6693;Parent=Msa1429840-mRNA-1
tig0026572 exon 30713 30778 30713 ID=Msa1429840-mRNA-1:exon:6694;Parent=Msa1429840-mRNA-1
tig0026572 exon 30880 32755 30880 ID=Msa1429840-mRNA-1:exon:6695;Parent=Msa1429840-mRNA-1
tig0026572 exon 33115 33608 33115 ID=Msa1429840-mRNA-1:exon:6696;Parent=Msa1429840-mRNA-1
tig0026572 exon 33641 33780 33641 ID=Msa1429840-mRNA-1:exon:6697;Parent=Msa1429840-mRNA-1
tig0026572 five_prime_UTR 30194 30326 30194 ID=Msa1429840-mRNA-1:five_prime_utr;Parent=Msa1429840-mRNA-1
tig0026572 CDS 30327 30416 30327 ID=Msa1429840-mRNA-1:cds;Parent=Msa1429840-mRNA-1
tig0026572 CDS 30713 30778 30713 ID=Msa1429840-mRNA-1:cds;Parent=Msa1429840-mRNA-1
tig0026572 CDS 30880 32755 30880 ID=Msa1429840-mRNA-1:cds;Parent=Msa1429840-mRNA-1
tig0026572 CDS 33115 33560 33115 ID=Msa1429840-mRNA-1:cds;Parent=Msa1429840-mRNA-1
tig0026572 three_prime_UTR 33561 33608 33561 ID=Msa1429840-mRNA-1:three_prime_utr;Parent=Msa1429840-mRNA-1
tig0026572 three_prime_UTR 33641 33780 33641 ID=Msa1429840-mRNA-1:three_prime_utr;Parent=Msa1429840-mRNA-1
Gene Sequence

>Msa1429840

ATGCCTCAGCTGCTACATGGGACACTTAAGGTAACCATAAGTGAAGTTGATAGGTTGCAAGCTGGATGTAATTTGGATTTCTGTCGAAAGGGAACAACACACAAGGGGAAGAGATTTCTAGCCCAAGTCAAGGGTTGTATCTTGTGCCGGCCTGAGATTGTTGGGACAAGACTCTATGCAACAGTTGATTTAGATAAAGCAAGGGTTGGACGAACCAGAATGATAGGGAATCAACCCTCAAACCCGAAATGGAATGAGACCTTTGAAATCTATTGTGCTCATTACATATCAAATATTGTATTCACTGTCAAAGATGATAATCCAATTGGTGCAACTCTAATCGGAAGAGCTTATATCCCTTCCGAGAAAATTCTCAAAGGACCTATAATTGACAGATGGGTTGAGATATTAGACGAAGAAGATCATCGTCCTGTACCAGGCGGTTCTAAAATTCGTGTCAGCATGCAATTCTCTAGTGTCATTGAGGATAGAAAATGTCTATGGTCTCAAGGAATACACATGCCATTTTTGGGAGTTCAGCGTACATTTTTCGATCAGAGACAAGGTTGTGGTGTTACTTTATACCAAGACGCTCATGTCCCTGACAGTGTTCATCCATGGATACCAATATCTGGAACAAAATATTATGTACCTGGAAGATGTTGGGAAGATATTTACAATGCCATTATGAATGCTAAAGTCTTTATTTACATAACTGGTTGGTCTGTTCATACAGAAATAACCTTGATTAGGGACCCAAACAAGCCAACAGAAAGTAGTATCACCCTAGGAGAAATACTCAAGAAAAAAGCCAATGAAGGTGTTAATGTTCTTGTGCTTGTTTGGGACGACAGAACCTCTGTTCCGGCTTTTAAGAAAGACGGTTTGATGGGAACACATGACCAAGAAACTGCAGAGTATTTTAAAAACACAAAGGTACGTTGTGTTTTATGTCCGCGTAACCCTGATGGTGGAAAAAGCATAGTTCAAGGTTTTGAAACTTCAACTATGTTCACTCATCACCAAAAGACTATAGTTGTTGATGGCCATCATGTTGAAGGATCGCAAAAGCGAACAGTTATCAGTTTCATAGGTGGTATTGATCTGTGTGATGGAAGATATGATACAATGGAACATCCTTTGTTTTCAACTTTGAACACAATCCATCATGATGATTTTCATCAACCAAATTTCCCAGGTGCTTCTATTAAGAAAGGTGGTCCTAGAGAACCGTGGCATGATATTCACTGCAAGCTAGAAGGACCTATTGCATGGGATGTGTTGTACAATTTTGAGCAGAGGTGGGAAAAGCAAGTCGGGAAGCAATTCCAACAATTCCTGTTCCCAATTCAAGAGCTTGATAGAATTCTAATCCATCCTTCTGATGCAATGTTATCAGAAGAAGATGGCGAAACTTGGAGCGTTCAGTTGTTTAGATCCATTGATGGTGGTGCTGTTTCTAACTTCCCTCAAGCACCAAATGAAGTTATTGAATTAGGCCTTGTTAGTGGAAAAGACAACATCATTGATAGAAGCATTCAAGATGCCTACATAAATGCTATACGAAGAGCAAAAAACTTCATCTACATTGAAAATCAGTATTTCCTAGGGAGTTCCTATGGTTGGCATTCAACTGATATTAAAGTTGAGGATATTGGTGCATTGCATCTCATACCAAAGGAGTTGTCATTGAAGATTGTTAGCAAAATTGAAGCTGGAGAGAGGTTTGTTGTGTATGTGGTGATTCCAATGTGGCCAGAAGGTATACCTGAGAGTGCATCAGTTCAGGCAATATTAGATTGGCAAAGAAGGACCATGGAGATGATGTATACTGAGATTGCTGATGCCATAAAAAAGAAGAATGTTGAAGCACACCCTCGTGATTATTTGACATTTTATTGTCTTGGTAAAAGGGAGAGTAAGAAAGATGGAGAGTACACTCCTCCAGAGCAACCAGAGCCTGACAGTGATTATCATAGAGCACAAAAGTCTAGAAGATTCATGATCTATGTTCATTCCAAGATGATGATAGTGGATGATGAATACATAGTGATTGGTTCAGCCAACATAAACCAAAGATCAATGGATGGTGGAAGAGACACAGAGATTGCAATGGGTGCATTTCAACCTCATCATCTAGCATCCACATCCAATGGCTCACAAAGACCACAAGGTCAAGTATATGGCTTCAGGAGAGCATTATGGTATGAACATATTGGAGACAACAGTGATGATTTTGACGAGCCTGAAAGTGTGGAATGCGTAAAACTTTTGAACCGTGTTGCTGAGAGGAATTGGAAATTATACTGTGATGATGCATTAGATGAAAGTGTAAATACTCACCTTTTACGGTATCCTGTTGAAGTGGGTGAAGATGGAAGTGTGACATCACTTTCAGGGATGCAATATTTTCCTGATACAATGGCTTTGATTCTTGGTTCAAAATCTGATTACCTTCCTCCCATACTTACCACCTAG
Protein sequence

>Msa1429840

MPQLLHGTLKVTISEVDRLQAGCNLDFCRKGTTHKGKRFLAQVKGCILCRPEIVGTRLYATVDLDKARVGRTRMIGNQPSNPKWNETFEIYCAHYISNIVFTVKDDNPIGATLIGRAYIPSEKILKGPIIDRWVEILDEEDHRPVPGGSKIRVSMQFSSVIEDRKCLWSQGIHMPFLGVQRTFFDQRQGCGVTLYQDAHVPDSVHPWIPISGTKYYVPGRCWEDIYNAIMNAKVFIYITGWSVHTEITLIRDPNKPTESSITLGEILKKKANEGVNVLVLVWDDRTSVPAFKKDGLMGTHDQETAEYFKNTKVRCVLCPRNPDGGKSIVQGFETSTMFTHHQKTIVVDGHHVEGSQKRTVISFIGGIDLCDGRYDTMEHPLFSTLNTIHHDDFHQPNFPGASIKKGGPREPWHDIHCKLEGPIAWDVLYNFEQRWEKQVGKQFQQFLFPIQELDRILIHPSDAMLSEEDGETWSVQLFRSIDGGAVSNFPQAPNEVIELGLVSGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSYGWHSTDIKVEDIGALHLIPKELSLKIVSKIEAGERFVVYVVIPMWPEGIPESASVQAILDWQRRTMEMMYTEIADAIKKKNVEAHPRDYLTFYCLGKRESKKDGEYTPPEQPEPDSDYHRAQKSRRFMIYVHSKMMIVDDEYIVIGSANINQRSMDGGRDTEIAMGAFQPHHLASTSNGSQRPQGQVYGFRRALWYEHIGDNSDDFDEPESVECVKLLNRVAERNWKLYCDDALDESVNTHLLRYPVEVGEDGSVTSLSGMQYFPDTMALILGSKSDYLPPILTT*