AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1432140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1432140 MtrunA17_Chr1g0167811 89.408 321 32 1 1 319 1 321 0.0 583
Msa1432140 MtrunA17_Chr1g0186661 45.604 364 142 14 1 325 1 347 1.06e-80 249
Msa1432140 MtrunA17_Chr1g0184171 39.871 311 158 12 30 322 39 338 1.65e-62 202
Msa1432140 MtrunA17_Chr7g0261091 35.669 314 165 9 32 312 13 322 2.76e-53 177
Msa1432140 MtrunA17_Chr5g0411081 29.151 271 138 9 25 272 50 289 3.35e-27 109
Msa1432140 MtrunA17_Chr8g0366561 36.875 160 95 4 31 190 57 210 3.97e-26 106
Msa1432140 MtrunA17_Chr5g0426781 33.679 193 100 8 34 214 60 236 3.35e-24 101
Msa1432140 MtrunA17_Chr8g0370171 29.630 162 95 3 9 170 5 147 3.44e-23 95.1
Msa1432140 MtrunA17_Chr5g0426881 33.117 154 93 1 13 166 45 188 7.50e-23 97.8
Msa1432140 MtrunA17_Chr4g0050041 35.976 164 81 5 18 166 43 197 2.14e-22 96.3
Msa1432140 MtrunA17_Chr3g0132491 36.842 133 67 4 32 163 62 178 6.48e-21 92.4
Msa1432140 MtrunA17_Chr4g0067591 51.316 76 32 1 91 166 79 149 9.90e-21 90.1
Msa1432140 MtrunA17_Chr4g0059281 31.724 145 83 3 31 167 42 178 2.94e-20 89.4
Msa1432140 MtrunA17_Chr7g0246761 31.034 174 96 7 24 192 2 156 3.12e-20 86.7
Msa1432140 MtrunA17_Chr5g0407431 29.630 135 89 2 36 170 26 154 6.94e-20 86.7
Msa1432140 MtrunA17_Chr6g0486651 32.911 158 91 6 15 168 20 166 7.20e-20 89.4
Msa1432140 MtrunA17_Chr7g0274741 47.945 73 38 0 91 163 69 141 7.47e-20 85.5
Msa1432140 MtrunA17_Chr1g0191601 34.328 134 73 3 32 163 49 169 9.56e-20 89.0
Msa1432140 MtrunA17_Chr8g0381731 54.667 75 31 2 92 166 148 219 9.96e-20 87.8
Msa1432140 MtrunA17_Chr7g0273051 31.034 145 87 1 19 163 31 162 1.10e-19 88.6
Msa1432140 MtrunA17_Chr5g0413841 31.210 157 96 3 19 166 34 187 3.58e-19 87.0
Msa1432140 MtrunA17_Chr2g0327791 47.297 74 38 1 93 166 108 180 6.37e-19 84.0
Msa1432140 MtrunA17_Chr2g0279431 50.667 75 34 1 92 166 135 206 1.00e-18 86.3
Msa1432140 MtrunA17_Chr8g0383871 27.136 199 121 7 7 192 27 214 1.73e-18 82.8
Msa1432140 MtrunA17_Chr7g0276591 45.455 77 38 1 93 169 77 149 3.21e-18 83.6
Msa1432140 MtrunA17_Chr2g0295571 42.222 90 49 2 77 166 78 164 3.39e-18 83.2
Msa1432140 MtrunA17_Chr8g0364881 50.000 72 33 1 92 163 68 136 3.83e-18 84.0
Msa1432140 MtrunA17_Chr3g0123631 42.857 77 44 0 91 167 45 121 4.19e-18 80.9
Msa1432140 MtrunA17_Chr3g0118071 38.462 130 72 5 39 163 20 146 5.61e-18 81.3
Msa1432140 MtrunA17_Chr3g0123641 42.857 77 44 0 91 167 45 121 1.23e-17 80.9
Msa1432140 MtrunA17_Chr3g0105441 41.975 81 46 1 87 166 97 177 1.39e-17 80.9
Msa1432140 MtrunA17_Chr5g0426871 30.667 150 90 4 19 167 47 183 1.55e-17 82.8
Msa1432140 MtrunA17_Chr2g0307411 31.915 141 61 4 32 166 34 145 1.94e-17 79.3
Msa1432140 MtrunA17_Chr1g0198201 33.088 136 82 3 28 163 4 130 2.41e-17 79.7
Msa1432140 MtrunA17_Chr1g0184631 44.444 72 40 0 92 163 68 139 2.69e-17 78.6
Msa1432140 MtrunA17_Chr3g0126931 33.103 145 75 5 77 203 87 227 2.90e-17 81.6
Msa1432140 MtrunA17_Chr7g0274711 44.444 72 40 0 92 163 67 138 3.29e-17 78.2
Msa1432140 MtrunA17_Chr8g0345361 44.444 81 41 2 88 168 88 164 4.04e-17 79.7
Msa1432140 MtrunA17_Chr4g0030831 39.394 99 55 3 72 166 60 157 4.71e-17 77.8
Msa1432140 MtrunA17_Chr6g0451161 34.307 137 82 3 31 163 69 201 6.94e-17 80.5
Msa1432140 MtrunA17_Chr8g0383941 27.273 132 79 2 33 164 63 177 8.96e-17 78.2
Msa1432140 MtrunA17_Chr8g0383861 27.108 166 104 4 7 164 27 183 9.32e-17 78.2
Msa1432140 MtrunA17_Chr5g0409211 28.058 139 85 3 32 167 14 140 1.05e-16 76.6
Msa1432140 MtrunA17_Chr3g0128771 27.407 135 84 4 32 166 44 164 1.22e-16 76.6
Msa1432140 MtrunA17_Chr3g0144241 30.597 134 78 3 43 170 29 153 1.25e-16 77.0
Msa1432140 MtrunA17_Chr2g0325141 30.469 128 85 2 42 166 10 136 1.25e-16 75.9
Msa1432140 MtrunA17_Chr3g0118061 42.045 88 49 2 77 163 60 146 1.35e-16 77.4
Msa1432140 MtrunA17_Chr8g0366521 30.667 150 88 5 18 163 11 148 1.63e-16 79.0
Msa1432140 MtrunA17_Chr6g0470751 28.467 137 85 2 31 167 49 172 1.99e-16 78.6
Msa1432140 MtrunA17_Chr3g0133001 42.667 75 40 2 92 166 130 201 2.52e-16 79.3
Msa1432140 MtrunA17_Chr8g0350741 42.857 70 38 2 94 163 64 131 2.83e-16 75.5
Msa1432140 MtrunA17_Chr8g0383921 36.364 99 57 3 88 182 107 203 3.17e-16 76.3
Msa1432140 MtrunA17_Chr5g0433861 31.387 137 83 4 31 163 19 148 3.89e-16 77.8
Msa1432140 MtrunA17_Chr5g0409201 29.054 148 88 4 32 176 15 148 4.65e-16 74.7
Msa1432140 MtrunA17_Chr8g0348151 45.833 72 34 1 92 163 90 156 4.72e-16 75.5
Msa1432140 MtrunA17_Chr5g0437361 37.363 91 56 1 82 172 65 154 4.85e-16 74.7
Msa1432140 MtrunA17_Chr2g0325161 34.694 98 55 2 92 186 91 182 6.16e-16 75.9
Msa1432140 MtrunA17_Chr8g0336881 27.941 136 92 1 31 166 21 150 6.43e-16 77.0
Msa1432140 MtrunA17_Chr7g0274721 43.836 73 37 1 91 163 67 135 1.42e-15 73.6
Msa1432140 MtrunA17_Chr5g0427431 40.741 81 39 2 89 166 131 205 1.52e-15 77.0
Msa1432140 MtrunA17_Chr7g0248831 41.667 72 41 1 92 163 80 150 2.00e-15 73.2
Msa1432140 MtrunA17_Chr3g0113811 33.613 119 66 4 62 168 36 153 2.31e-15 72.8
Msa1432140 MtrunA17_Chr7g0244851 31.148 122 77 3 43 163 34 149 2.62e-15 72.8
Msa1432140 MtrunA17_Chr8g0388201 40.426 94 48 3 75 163 299 389 3.43e-15 75.9
Msa1432140 MtrunA17_Chr3g0118091 37.778 90 52 2 92 181 40 125 4.52e-15 70.9
Msa1432140 MtrunA17_Chr8g0383881 37.333 75 45 1 90 164 101 173 5.10e-15 73.2
Msa1432140 MtrunA17_Chr5g0396611 50.000 72 35 1 92 163 312 382 5.25e-15 75.1
Msa1432140 MtrunA17_Chr5g0396591 50.000 74 32 2 92 163 212 282 7.22e-15 73.9
Msa1432140 MtrunA17_Chr2g0301871 40.909 66 39 0 101 166 63 128 7.59e-15 73.9
Msa1432140 MtrunA17_Chr8g0383901 38.947 95 52 3 102 192 114 206 8.87e-15 72.4
Msa1432140 MtrunA17_Chr8g0369571 29.078 141 82 5 28 167 13 136 1.13e-14 71.2
Msa1432140 MtrunA17_Chr1g0157091 31.667 120 76 1 57 176 13 126 1.19e-14 69.7
Msa1432140 MtrunA17_Chr7g0248841 41.026 78 35 2 92 163 74 146 1.28e-14 70.5
Msa1432140 MtrunA17_Chr3g0081611 41.667 72 41 1 92 163 80 150 1.31e-14 70.9
Msa1432140 MtrunA17_Chr4g0073141 41.333 75 36 2 92 163 117 186 2.08e-14 73.9
Msa1432140 MtrunA17_Chr5g0396601 50.000 74 32 2 92 163 322 392 2.13e-14 73.6
Msa1432140 MtrunA17_Chr7g0269491 37.634 93 57 1 74 166 48 139 2.21e-14 70.9
Msa1432140 MtrunA17_Chr7g0275601 34.545 110 61 3 57 163 49 150 3.25e-14 69.7
Msa1432140 MtrunA17_Chr3g0129671 23.837 172 110 4 3 164 19 179 3.26e-14 70.9
Msa1432140 MtrunA17_Chr1g0197031 38.356 73 44 1 92 163 72 144 3.98e-14 69.3
Msa1432140 MtrunA17_Chr7g0274731 42.466 73 38 1 91 163 88 156 5.42e-14 69.7
Msa1432140 MtrunA17_Chr8g0383931 36.842 95 54 3 102 192 124 216 6.48e-14 70.1
Msa1432140 MtrunA17_Chr5g0444161 36.709 79 43 2 91 166 156 230 7.24e-14 70.1
Msa1432140 MtrunA17_Chr1g0177041 37.500 72 45 0 92 163 74 145 7.88e-14 68.6
Msa1432140 MtrunA17_Chr5g0430781 41.096 73 37 2 91 163 86 152 8.75e-14 69.7
Msa1432140 MtrunA17_Chr8g0345401 42.466 73 37 1 100 167 23 95 1.38e-13 67.4
Msa1432140 MtrunA17_Chr4g0010171 36.000 100 61 2 71 170 66 162 1.61e-13 68.2
Msa1432140 MtrunA17_Chr4g0027761 33.333 99 52 3 65 163 81 165 1.92e-13 68.2
Msa1432140 MtrunA17_Chr5g0444141 36.709 79 43 2 91 166 191 265 7.06e-13 67.8
Msa1432140 MtrunA17_Chr3g0143301 38.158 76 41 1 91 166 47 116 8.85e-13 64.7
Msa1432140 MtrunA17_Chr5g0406031 36.842 76 47 1 91 166 160 234 9.47e-13 67.0
Msa1432140 MtrunA17_Chr7g0237871 28.788 132 86 3 36 167 81 204 1.10e-12 66.6
Msa1432140 MtrunA17_Chr4g0013561 30.476 105 63 3 59 163 58 152 1.21e-12 66.6
Msa1432140 MtrunA17_Chr8g0345381 42.623 61 34 1 121 180 53 113 1.24e-12 64.7
Msa1432140 MtrunA17_Chr3g0117821 32.000 100 65 2 69 166 32 130 3.41e-12 63.5
Msa1432140 MtrunA17_Chr1g0197021 39.394 66 39 1 99 163 76 141 3.47e-12 63.9
Msa1432140 MtrunA17_Chr8g0343571 38.667 75 38 1 109 183 73 139 4.06e-12 63.9
Msa1432140 MtrunA17_Chr3g0120011 40.244 82 43 2 84 163 84 161 4.24e-12 64.7
Msa1432140 MtrunA17_Chr8g0388231 41.250 80 42 2 86 163 303 379 6.22e-12 65.9
Msa1432140 MtrunA17_Chr3g0118101 31.915 94 58 2 92 181 87 178 6.76e-12 63.2
Msa1432140 MtrunA17_Chr1g0203541 32.051 78 51 1 91 166 104 181 6.88e-12 63.9
Msa1432140 MtrunA17_Chr1g0211831 36.585 82 49 2 88 169 257 335 8.70e-12 65.1
Msa1432140 MtrunA17_Chr8g0371291 38.356 73 38 1 92 164 158 223 1.04e-11 63.5
Msa1432140 MtrunA17_Chr8g0347461 31.776 107 67 3 95 197 114 218 1.62e-11 63.9
Msa1432140 MtrunA17_Chr2g0317671 45.312 64 33 2 102 163 138 201 2.44e-11 62.4
Msa1432140 MtrunA17_Chr3g0118111 29.630 81 57 0 92 172 84 164 2.82e-11 61.6
Msa1432140 MtrunA17_Chr2g0297011 27.885 104 72 1 75 178 59 159 3.74e-11 60.8
Msa1432140 MtrunA17_Chr2g0300331 37.975 79 46 1 91 166 136 214 7.41e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1432140 AT1G04360.1 41.261 349 151 13 27 326 38 381 4.31e-64 207
Msa1432140 AT5G43420.1 40.924 303 113 12 27 280 31 316 2.25e-60 197
Msa1432140 AT3G03550.1 28.829 222 136 6 31 238 58 271 8.64e-28 111
Msa1432140 AT4G17905.1 34.254 181 107 3 31 205 60 234 1.06e-27 110
Msa1432140 AT4G17905.2 34.254 181 107 3 31 205 86 260 3.13e-27 109
Msa1432140 AT5G17600.1 35.075 134 83 2 34 167 59 188 1.08e-22 97.4
Msa1432140 AT1G72220.1 35.503 169 93 4 20 183 76 233 1.55e-21 94.7
Msa1432140 AT4G28890.1 32.402 179 94 6 9 174 12 176 4.18e-21 93.6
Msa1432140 AT2G20030.1 32.544 169 100 5 11 174 18 177 7.01e-21 92.8
Msa1432140 AT3G18773.1 32.886 149 85 4 15 163 39 172 8.62e-21 89.4
Msa1432140 AT3G16720.1 31.707 164 88 3 79 233 81 229 1.09e-20 90.9
Msa1432140 AT4G40070.1 32.468 154 93 3 14 167 28 170 1.10e-20 91.3
Msa1432140 AT2G34990.1 34.532 139 79 5 27 163 10 138 1.28e-20 90.9
Msa1432140 AT2G34990.2 34.532 139 79 5 27 163 32 160 1.67e-20 90.9
Msa1432140 AT1G23980.1 33.094 139 84 2 31 163 51 186 1.84e-20 91.3
Msa1432140 AT1G72200.1 30.827 133 85 1 31 163 61 186 4.86e-20 90.5
Msa1432140 AT3G05200.1 29.375 160 95 5 13 167 28 174 6.09e-20 90.1
Msa1432140 AT4G09100.1 49.351 77 37 2 91 166 55 130 1.38e-19 84.0
Msa1432140 AT4G09110.1 32.168 143 79 4 28 168 44 170 1.46e-19 87.8
Msa1432140 AT1G76410.1 30.469 128 87 1 43 170 28 153 3.68e-19 84.0
Msa1432140 AT2G35000.1 32.414 145 89 3 29 171 48 185 5.73e-19 87.0
Msa1432140 AT4G09120.1 48.750 80 37 2 91 168 93 170 7.85e-19 86.3
Msa1432140 AT5G10380.1 50.000 82 34 3 92 171 109 185 9.21e-19 85.5
Msa1432140 AT4G17920.1 29.870 154 92 4 18 166 13 155 9.63e-19 85.1
Msa1432140 AT1G35330.1 34.074 135 77 4 31 163 47 171 1.13e-18 85.5
Msa1432140 AT5G40250.1 32.432 148 80 3 31 163 43 185 2.34e-18 85.1
Msa1432140 AT2G27940.1 33.803 142 87 3 24 163 46 182 2.66e-18 83.2
Msa1432140 AT5G57750.1 30.935 139 82 3 31 163 34 164 6.32e-18 81.3
Msa1432140 AT1G49220.1 34.959 123 73 3 44 163 59 177 6.50e-18 82.4
Msa1432140 AT1G20823.1 26.923 156 98 4 19 170 17 160 1.91e-17 79.7
Msa1432140 AT2G17450.1 26.786 168 101 4 16 179 11 160 1.95e-17 79.3
Msa1432140 AT3G48030.2 43.902 82 36 2 92 169 102 177 4.67e-17 80.1
Msa1432140 AT5G27420.1 31.783 129 77 3 39 167 53 170 4.97e-17 81.3
Msa1432140 AT2G42360.1 28.649 185 102 4 19 203 22 176 5.64e-17 79.3
Msa1432140 AT3G48030.1 43.902 82 36 2 92 169 180 255 6.86e-17 80.9
Msa1432140 AT4G15975.1 36.458 96 57 1 68 163 27 118 7.25e-17 79.0
Msa1432140 AT5G46650.1 31.206 141 88 3 31 166 24 160 7.42e-17 80.1
Msa1432140 AT5G05810.1 32.143 140 89 4 30 166 55 191 8.19e-17 80.9
Msa1432140 AT2G46160.1 42.857 77 39 1 92 163 104 180 9.16e-17 78.2
Msa1432140 AT2G46160.2 42.857 77 39 1 92 163 104 180 9.16e-17 78.2
Msa1432140 AT2G42350.1 31.285 179 95 7 70 233 51 216 1.08e-16 78.2
Msa1432140 AT4G09130.1 43.750 80 41 2 91 168 90 167 1.14e-16 80.1
Msa1432140 AT4G30400.1 45.833 72 37 1 92 163 107 176 1.17e-16 80.9
Msa1432140 AT3G14320.1 39.024 82 46 2 89 170 60 137 1.55e-16 77.4
Msa1432140 AT1G22500.1 31.469 143 64 3 33 163 40 160 1.74e-16 79.7
Msa1432140 AT4G33565.1 45.333 75 38 1 92 166 191 262 1.90e-16 79.7
Msa1432140 AT1G49210.1 34.127 126 80 2 39 163 54 177 2.42e-16 77.4
Msa1432140 AT1G49200.1 33.333 123 75 3 44 163 60 178 2.56e-16 77.4
Msa1432140 AT2G35910.1 44.444 72 37 1 92 163 121 189 4.93e-16 76.3
Msa1432140 AT3G61550.1 41.975 81 37 2 92 163 99 178 5.65e-16 75.9
Msa1432140 AT2G18650.1 45.205 73 36 2 92 163 99 168 5.83e-16 78.6
Msa1432140 AT3G62690.1 40.000 80 45 1 93 172 88 164 6.75e-16 76.6
Msa1432140 AT2G47560.1 38.095 84 51 1 83 166 71 153 7.28e-16 75.9
Msa1432140 AT2G35420.1 28.873 142 78 5 29 163 20 145 9.66e-16 76.3
Msa1432140 AT3G10910.1 27.168 173 93 7 38 195 23 177 1.24e-15 74.3
Msa1432140 AT5G01880.1 37.500 88 50 2 80 166 67 150 1.24e-15 73.6
Msa1432140 AT5G06490.1 37.755 98 52 3 70 163 79 171 2.55e-15 73.9
Msa1432140 AT5G05280.1 27.407 135 81 4 32 166 41 158 2.81e-15 73.2
Msa1432140 AT3G18930.2 37.778 90 48 2 84 166 113 201 4.01e-15 75.9
Msa1432140 AT3G18930.3 37.778 90 48 2 84 166 113 201 4.01e-15 75.9
Msa1432140 AT3G18930.1 37.778 90 48 2 84 166 113 201 4.01e-15 75.9
Msa1432140 AT2G46493.1 33.858 127 78 3 38 163 59 180 4.16e-15 72.8
Msa1432140 AT1G53820.1 37.000 100 52 2 67 166 77 165 5.69e-15 74.7
Msa1432140 AT4G10160.1 29.730 111 62 2 82 192 62 156 5.84e-15 73.6
Msa1432140 AT4G10150.1 27.059 170 89 6 30 192 29 170 7.65e-15 73.2
Msa1432140 AT5G53110.1 32.374 139 75 4 27 163 251 372 8.56e-15 75.1
Msa1432140 AT1G32361.1 36.782 87 50 2 89 175 127 208 9.66e-15 74.3
Msa1432140 AT5G07040.1 28.395 162 93 4 13 170 1 143 1.03e-14 71.2
Msa1432140 AT3G19140.1 31.250 144 80 5 42 184 13 138 1.56e-14 70.1
Msa1432140 AT4G35840.1 34.091 88 58 0 79 166 149 236 2.42e-14 72.0
Msa1432140 AT3G60220.1 42.029 69 40 0 95 163 91 159 2.99e-14 72.8
Msa1432140 AT1G49230.1 27.481 131 92 2 41 171 54 181 7.36e-14 70.1
Msa1432140 AT2G34000.1 27.820 133 86 3 33 164 11 134 7.58e-14 68.6
Msa1432140 AT3G20395.1 31.897 116 65 3 52 167 116 217 1.01e-13 69.7
Msa1432140 AT1G72310.1 36.000 75 44 1 92 166 102 172 1.16e-13 71.2
Msa1432140 AT1G80400.1 37.000 100 41 3 88 166 301 399 1.81e-13 71.2
Msa1432140 AT1G33480.1 32.292 96 61 1 85 180 68 159 2.29e-13 69.3
Msa1432140 AT4G35480.1 29.825 114 66 2 65 164 43 156 3.21e-13 68.2
Msa1432140 AT5G66070.5 38.462 78 43 2 87 164 147 219 3.78e-13 68.2
Msa1432140 AT5G66070.1 38.462 78 43 2 87 164 147 219 3.78e-13 68.2
Msa1432140 AT5G66070.2 38.462 78 43 2 87 164 171 243 8.24e-13 67.8
Msa1432140 AT2G17730.1 32.941 85 57 0 82 166 157 241 8.29e-13 67.4
Msa1432140 AT5G58580.1 36.782 87 53 1 80 166 99 183 1.45e-12 67.8
Msa1432140 AT2G46495.2 38.750 80 49 0 92 171 263 342 1.71e-12 67.8
Msa1432140 AT2G46494.1 44.595 74 38 2 91 163 287 358 2.00e-12 67.8
Msa1432140 AT2G46495.1 38.750 80 49 0 92 171 291 370 2.01e-12 67.8
Msa1432140 AT1G53010.1 31.250 160 78 8 32 166 23 175 2.60e-12 65.1
Msa1432140 AT1G28040.1 28.902 173 89 7 2 163 146 295 3.03e-12 66.6
Msa1432140 AT2G18670.1 32.500 80 52 1 92 171 83 160 6.11e-12 63.9
Msa1432140 AT5G42200.1 27.206 136 78 4 32 167 36 150 7.75e-12 63.2
Msa1432140 AT5G47610.1 35.821 67 41 1 100 166 88 152 1.46e-11 62.4
Msa1432140 AT2G25410.1 35.000 80 52 0 92 171 298 377 2.13e-11 64.7
Msa1432140 AT1G74410.1 32.203 118 49 4 74 166 110 221 6.33e-11 62.0
Msa1432140 AT2G37580.1 36.765 68 43 0 107 174 128 195 6.94e-11 62.0
Msa1432140 AT4G24015.1 47.368 57 29 1 114 169 97 153 8.28e-11 60.5
Msa1432140 AT3G19910.1 34.884 86 53 2 88 173 257 339 9.96e-11 62.4

Find 40 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCACAATCAAGATGATTT+TGG 0.122480 tig0026954:+39166 Msa1432140:intergenic
GAGAAAGACTATGGTGAATT+AGG 0.240210 tig0026954:-38901 Msa1432140:CDS
TGAAATAAATGGTTGCAATT+TGG 0.290797 tig0026954:+38814 Msa1432140:intergenic
CACAATCAATGTGAAATAAA+TGG 0.300719 tig0026954:+38803 Msa1432140:intergenic
ATTGAGAAAGACCTTCTAAT+TGG 0.305044 tig0026954:+38436 Msa1432140:intergenic
CCTACTACAATGATTACTAT+TGG 0.329488 tig0026954:+39135 Msa1432140:intergenic
TTCTAGTTCAACCAATTAGA+AGG 0.340761 tig0026954:-38447 Msa1432140:CDS
ACATTGATTGTGTTGATGTT+TGG 0.348549 tig0026954:-38792 Msa1432140:CDS
CCAATAGTAATCATTGTAGT+AGG 0.351049 tig0026954:-39135 Msa1432140:CDS
CAACCTAGTTTGCATGTTCA+TGG 0.351504 tig0026954:-38385 Msa1432140:CDS
AGATGAAAGAGGTAAAGAAT+TGG 0.372927 tig0026954:-38560 Msa1432140:CDS
TTTCAAGGGTCCAAATGAAT+TGG 0.374461 tig0026954:+38265 Msa1432140:intergenic
TGATCAAATGCTGCTTCAAT+TGG 0.375168 tig0026954:-39068 Msa1432140:CDS
AAGGAAAAGAATGATCTCTT+AGG 0.393134 tig0026954:+38322 Msa1432140:intergenic
TACAAAGAAGAGAAAGACTA+TGG 0.419059 tig0026954:-38910 Msa1432140:CDS
TGCAAATAAGGTGAAGGTCT+TGG 0.431537 tig0026954:+38688 Msa1432140:intergenic
TCTTCTTTGTAATGAATCAC+TGG 0.437626 tig0026954:+38922 Msa1432140:intergenic
CAAAATGTTGAAAACATCAT+TGG 0.439598 tig0026954:-38661 Msa1432140:CDS
AATTAGAAGGTCTTTCTCAA+TGG 0.453667 tig0026954:-38434 Msa1432140:CDS
GTTAGTTCCATTGAAGTTTG+TGG 0.456288 tig0026954:-38361 Msa1432140:CDS
ACTATCACCACAAACTTCAA+TGG 0.458585 tig0026954:+38354 Msa1432140:intergenic
AACCTAGTTTGCATGTTCAT+GGG 0.461073 tig0026954:-38384 Msa1432140:CDS
GATTTCTTGTGTGAATCTCT+TGG 0.478795 tig0026954:+38532 Msa1432140:intergenic
TGTATTAAAGACAAAGATGA+TGG 0.482467 tig0026954:-38472 Msa1432140:CDS
TTTGATCACAAAGGTGTAGT+AGG 0.482721 tig0026954:+39083 Msa1432140:intergenic
TGAAAGAGGTAAAGAATTGG+AGG 0.511082 tig0026954:-38557 Msa1432140:CDS
GAGTGACAACATGCTCAACT+TGG 0.515758 tig0026954:+38710 Msa1432140:intergenic
AATCATTGTAGTAGGAATGA+TGG 0.520403 tig0026954:-39127 Msa1432140:CDS
ACCCCATGAACATGCAAACT+AGG 0.537002 tig0026954:+38382 Msa1432140:intergenic
CATGAAAGAAGAGATGAAAG+AGG 0.539247 tig0026954:-38571 Msa1432140:CDS
TGGCTTTGCAAATAAGGTGA+AGG 0.542682 tig0026954:+38682 Msa1432140:intergenic
GCATAGAGGATTAGAACAAT+CGG 0.544428 tig0026954:-38959 Msa1432140:CDS
AATAATGAAAATGACCATGA+AGG 0.566631 tig0026954:-38604 Msa1432140:CDS
ACCTAGTTTGCATGTTCATG+GGG 0.568859 tig0026954:-38383 Msa1432140:CDS
AATGTTGAAAACATCATTGG+TGG 0.585029 tig0026954:-38658 Msa1432140:CDS
TGATCTACTTCCATGTCCAA+AGG 0.586042 tig0026954:+38303 Msa1432140:intergenic
ACCCAAAATCATCTTGATTG+TGG 0.603547 tig0026954:-39168 Msa1432140:CDS
GAAGCAGCATTTGATCACAA+AGG 0.695774 tig0026954:+39074 Msa1432140:intergenic
TCAATGACATCGGAGCATAG+AGG 0.698361 tig0026954:-38973 Msa1432140:CDS
TACGTCGTACTCAATGACAT+CGG 0.715236 tig0026954:-38983 Msa1432140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTTGTTGTAATTGATT+TGG - tig0026954:38863-38882 Msa1432140:CDS 20.0%
!!! AGATTTTGTTGTAATTGATT+TGG - tig0026954:38863-38882 Msa1432140:CDS 20.0%
! TGAAATAAATGGTTGCAATT+TGG + tig0026954:38681-38700 Msa1432140:intergenic 25.0%
! CACAATCAATGTGAAATAAA+TGG + tig0026954:38692-38711 Msa1432140:intergenic 25.0%
!! CAATGATGTTTTCAACATTT+TGG + tig0026954:38833-38852 Msa1432140:intergenic 25.0%
! CAAAATGTTGAAAACATCAT+TGG - tig0026954:38831-38850 Msa1432140:CDS 25.0%
! AATAATGAAAATGACCATGA+AGG - tig0026954:38888-38907 Msa1432140:CDS 25.0%
! TGTATTAAAGACAAAGATGA+TGG - tig0026954:39020-39039 Msa1432140:CDS 25.0%
!!! TGTTGTTTTACCAATTCATT+TGG - tig0026954:39217-39236 Msa1432140:CDS 25.0%
! TGAAATAAATGGTTGCAATT+TGG + tig0026954:38681-38700 Msa1432140:intergenic 25.0%
! CACAATCAATGTGAAATAAA+TGG + tig0026954:38692-38711 Msa1432140:intergenic 25.0%
!! CAATGATGTTTTCAACATTT+TGG + tig0026954:38833-38852 Msa1432140:intergenic 25.0%
! CAAAATGTTGAAAACATCAT+TGG - tig0026954:38831-38850 Msa1432140:CDS 25.0%
! AATAATGAAAATGACCATGA+AGG - tig0026954:38888-38907 Msa1432140:CDS 25.0%
! TGTATTAAAGACAAAGATGA+TGG - tig0026954:39020-39039 Msa1432140:CDS 25.0%
!!! TGTTGTTTTACCAATTCATT+TGG - tig0026954:39217-39236 Msa1432140:CDS 25.0%
! ACCACAATCAAGATGATTTT+GGG + tig0026954:38328-38347 Msa1432140:intergenic 30.0%
! TACCACAATCAAGATGATTT+TGG + tig0026954:38329-38348 Msa1432140:intergenic 30.0%
CCTACTACAATGATTACTAT+TGG + tig0026954:38360-38379 Msa1432140:intergenic 30.0%
CCAATAGTAATCATTGTAGT+AGG - tig0026954:38357-38376 Msa1432140:CDS 30.0%
AATCATTGTAGTAGGAATGA+TGG - tig0026954:38365-38384 Msa1432140:CDS 30.0%
TCTTCTTTGTAATGAATCAC+TGG + tig0026954:38573-38592 Msa1432140:intergenic 30.0%
TACAAAGAAGAGAAAGACTA+TGG - tig0026954:38582-38601 Msa1432140:CDS 30.0%
! ACATTGATTGTGTTGATGTT+TGG - tig0026954:38700-38719 Msa1432140:CDS 30.0%
! GAAACTTTTCTTCTACAAAG+TGG + tig0026954:38747-38766 Msa1432140:intergenic 30.0%
!!! ACATTTTGGCTTTGCAAATA+AGG + tig0026954:38819-38838 Msa1432140:intergenic 30.0%
AATGTTGAAAACATCATTGG+TGG - tig0026954:38834-38853 Msa1432140:CDS 30.0%
AGATGAAAGAGGTAAAGAAT+TGG - tig0026954:38932-38951 Msa1432140:CDS 30.0%
!! TTCTAGTTCAACCAATTAGA+AGG - tig0026954:39045-39064 Msa1432140:CDS 30.0%
ATTGAGAAAGACCTTCTAAT+TGG + tig0026954:39059-39078 Msa1432140:intergenic 30.0%
AATTAGAAGGTCTTTCTCAA+TGG - tig0026954:39058-39077 Msa1432140:CDS 30.0%
AAGGAAAAGAATGATCTCTT+AGG + tig0026954:39173-39192 Msa1432140:intergenic 30.0%
! AAGAGATCATTCTTTTCCTT+TGG - tig0026954:39173-39192 Msa1432140:CDS 30.0%
! ACCACAATCAAGATGATTTT+GGG + tig0026954:38328-38347 Msa1432140:intergenic 30.0%
! TACCACAATCAAGATGATTT+TGG + tig0026954:38329-38348 Msa1432140:intergenic 30.0%
CCTACTACAATGATTACTAT+TGG + tig0026954:38360-38379 Msa1432140:intergenic 30.0%
CCAATAGTAATCATTGTAGT+AGG - tig0026954:38357-38376 Msa1432140:CDS 30.0%
AATCATTGTAGTAGGAATGA+TGG - tig0026954:38365-38384 Msa1432140:CDS 30.0%
TCTTCTTTGTAATGAATCAC+TGG + tig0026954:38573-38592 Msa1432140:intergenic 30.0%
TACAAAGAAGAGAAAGACTA+TGG - tig0026954:38582-38601 Msa1432140:CDS 30.0%
! ACATTGATTGTGTTGATGTT+TGG - tig0026954:38700-38719 Msa1432140:CDS 30.0%
! GAAACTTTTCTTCTACAAAG+TGG + tig0026954:38747-38766 Msa1432140:intergenic 30.0%
!!! ACATTTTGGCTTTGCAAATA+AGG + tig0026954:38819-38838 Msa1432140:intergenic 30.0%
AATGTTGAAAACATCATTGG+TGG - tig0026954:38834-38853 Msa1432140:CDS 30.0%
AGATGAAAGAGGTAAAGAAT+TGG - tig0026954:38932-38951 Msa1432140:CDS 30.0%
!! TTCTAGTTCAACCAATTAGA+AGG - tig0026954:39045-39064 Msa1432140:CDS 30.0%
ATTGAGAAAGACCTTCTAAT+TGG + tig0026954:39059-39078 Msa1432140:intergenic 30.0%
AATTAGAAGGTCTTTCTCAA+TGG - tig0026954:39058-39077 Msa1432140:CDS 30.0%
AAGGAAAAGAATGATCTCTT+AGG + tig0026954:39173-39192 Msa1432140:intergenic 30.0%
! AAGAGATCATTCTTTTCCTT+TGG - tig0026954:39173-39192 Msa1432140:CDS 30.0%
!! CAATCAAGATGATTTTGGGT+AGG + tig0026954:38324-38343 None:intergenic 35.0%
ACCCAAAATCATCTTGATTG+TGG - tig0026954:38324-38343 Msa1432140:CDS 35.0%
! GGCAACATTTTTCTTGCTAA+TGG - tig0026954:38386-38405 Msa1432140:CDS 35.0%
! TTTGATCACAAAGGTGTAGT+AGG + tig0026954:38412-38431 Msa1432140:intergenic 35.0%
! TGATCAAATGCTGCTTCAAT+TGG - tig0026954:38424-38443 Msa1432140:CDS 35.0%
GCATAGAGGATTAGAACAAT+CGG - tig0026954:38533-38552 Msa1432140:CDS 35.0%
GAGAAAGACTATGGTGAATT+AGG - tig0026954:38591-38610 Msa1432140:CDS 35.0%
CATGAAAGAAGAGATGAAAG+AGG - tig0026954:38921-38940 Msa1432140:CDS 35.0%
TGAAAGAGGTAAAGAATTGG+AGG - tig0026954:38935-38954 Msa1432140:CDS 35.0%
GATTTCTTGTGTGAATCTCT+TGG + tig0026954:38963-38982 Msa1432140:intergenic 35.0%
!! CTTCAAAAAGTGTCTAGCAT+GGG - tig0026954:38990-39009 Msa1432140:CDS 35.0%
!! TTCAAAAAGTGTCTAGCATG+GGG - tig0026954:38991-39010 Msa1432140:CDS 35.0%
! AACCTAGTTTGCATGTTCAT+GGG - tig0026954:39108-39127 Msa1432140:CDS 35.0%
GTTAGTTCCATTGAAGTTTG+TGG - tig0026954:39131-39150 Msa1432140:CDS 35.0%
ACTATCACCACAAACTTCAA+TGG + tig0026954:39141-39160 Msa1432140:intergenic 35.0%
! TCATTCTTTTCCTTTGGACA+TGG - tig0026954:39179-39198 Msa1432140:CDS 35.0%
!! CAATCAAGATGATTTTGGGT+AGG + tig0026954:38324-38343 Msa1432140:intergenic 35.0%
ACCCAAAATCATCTTGATTG+TGG - tig0026954:38324-38343 Msa1432140:CDS 35.0%
! GGCAACATTTTTCTTGCTAA+TGG - tig0026954:38386-38405 Msa1432140:CDS 35.0%
! TTTGATCACAAAGGTGTAGT+AGG + tig0026954:38412-38431 Msa1432140:intergenic 35.0%
! TGATCAAATGCTGCTTCAAT+TGG - tig0026954:38424-38443 Msa1432140:CDS 35.0%
GCATAGAGGATTAGAACAAT+CGG - tig0026954:38533-38552 Msa1432140:CDS 35.0%
GAGAAAGACTATGGTGAATT+AGG - tig0026954:38591-38610 Msa1432140:CDS 35.0%
CATGAAAGAAGAGATGAAAG+AGG - tig0026954:38921-38940 Msa1432140:CDS 35.0%
TGAAAGAGGTAAAGAATTGG+AGG - tig0026954:38935-38954 Msa1432140:CDS 35.0%
GATTTCTTGTGTGAATCTCT+TGG + tig0026954:38963-38982 Msa1432140:intergenic 35.0%
!! CTTCAAAAAGTGTCTAGCAT+GGG - tig0026954:38990-39009 Msa1432140:CDS 35.0%
!! TTCAAAAAGTGTCTAGCATG+GGG - tig0026954:38991-39010 Msa1432140:CDS 35.0%
! AACCTAGTTTGCATGTTCAT+GGG - tig0026954:39108-39127 Msa1432140:CDS 35.0%
GTTAGTTCCATTGAAGTTTG+TGG - tig0026954:39131-39150 Msa1432140:CDS 35.0%
ACTATCACCACAAACTTCAA+TGG + tig0026954:39141-39160 Msa1432140:intergenic 35.0%
! TCATTCTTTTCCTTTGGACA+TGG - tig0026954:39179-39198 Msa1432140:CDS 35.0%
!! TCAAGATGATTTTGGGTAGG+TGG + tig0026954:38321-38340 None:intergenic 40.0%
GAAGCAGCATTTGATCACAA+AGG + tig0026954:38421-38440 Msa1432140:intergenic 40.0%
TACGTCGTACTCAATGACAT+CGG - tig0026954:38509-38528 Msa1432140:CDS 40.0%
TGCAAATAAGGTGAAGGTCT+TGG + tig0026954:38807-38826 Msa1432140:intergenic 40.0%
! TGGCTTTGCAAATAAGGTGA+AGG + tig0026954:38813-38832 Msa1432140:intergenic 40.0%
!! CATGCAAGTTTTGACCTTCA+TGG + tig0026954:38905-38924 Msa1432140:intergenic 40.0%
!! GCTTCAAAAAGTGTCTAGCA+TGG - tig0026954:38989-39008 Msa1432140:CDS 40.0%
!! TCAAAAAGTGTCTAGCATGG+GGG - tig0026954:38992-39011 Msa1432140:CDS 40.0%
! CAACCTAGTTTGCATGTTCA+TGG - tig0026954:39107-39126 Msa1432140:CDS 40.0%
! ACCTAGTTTGCATGTTCATG+GGG - tig0026954:39109-39128 Msa1432140:CDS 40.0%
TGATCTACTTCCATGTCCAA+AGG + tig0026954:39192-39211 Msa1432140:intergenic 40.0%
!! TCAAGATGATTTTGGGTAGG+TGG + tig0026954:38321-38340 Msa1432140:intergenic 40.0%
GAAGCAGCATTTGATCACAA+AGG + tig0026954:38421-38440 Msa1432140:intergenic 40.0%
TACGTCGTACTCAATGACAT+CGG - tig0026954:38509-38528 Msa1432140:CDS 40.0%
TGCAAATAAGGTGAAGGTCT+TGG + tig0026954:38807-38826 Msa1432140:intergenic 40.0%
! TGGCTTTGCAAATAAGGTGA+AGG + tig0026954:38813-38832 Msa1432140:intergenic 40.0%
!! CATGCAAGTTTTGACCTTCA+TGG + tig0026954:38905-38924 Msa1432140:intergenic 40.0%
!! GCTTCAAAAAGTGTCTAGCA+TGG - tig0026954:38989-39008 Msa1432140:CDS 40.0%
!! TCAAAAAGTGTCTAGCATGG+GGG - tig0026954:38992-39011 Msa1432140:CDS 40.0%
! CAACCTAGTTTGCATGTTCA+TGG - tig0026954:39107-39126 Msa1432140:CDS 40.0%
! ACCTAGTTTGCATGTTCATG+GGG - tig0026954:39109-39128 Msa1432140:CDS 40.0%
TGATCTACTTCCATGTCCAA+AGG + tig0026954:39192-39211 Msa1432140:intergenic 40.0%
TCAATGACATCGGAGCATAG+AGG - tig0026954:38519-38538 Msa1432140:CDS 45.0%
GAGTGACAACATGCTCAACT+TGG + tig0026954:38785-38804 Msa1432140:intergenic 45.0%
ACCCCATGAACATGCAAACT+AGG + tig0026954:39113-39132 Msa1432140:intergenic 45.0%
TCAATGACATCGGAGCATAG+AGG - tig0026954:38519-38538 Msa1432140:CDS 45.0%
GAGTGACAACATGCTCAACT+TGG + tig0026954:38785-38804 Msa1432140:intergenic 45.0%
ACCCCATGAACATGCAAACT+AGG + tig0026954:39113-39132 Msa1432140:intergenic 45.0%
Chromosome Type Strat End Strand Name
tig0026954 gene 38267 39247 38267 ID=Msa1432140;Name=Msa1432140
tig0026954 mRNA 38267 39247 38267 ID=Msa1432140-mRNA-1;Parent=Msa1432140;Name=Msa1432140-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|326
tig0026954 exon 38267 39247 38267 ID=Msa1432140-mRNA-1:exon:7918;Parent=Msa1432140-mRNA-1
tig0026954 CDS 38267 39247 38267 ID=Msa1432140-mRNA-1:cds;Parent=Msa1432140-mRNA-1
Gene Sequence

>Msa1432140

ATGGGTTTTGTTAACAAACAACACTTGATCAATCATGTGTCGCAAGCTCATCCACCTACCCAAAATCATCTTGATTGTGGTACAAATTTTCCAATAGTAATCATTGTAGTAGGAATGATGGCAACATTTTTCTTGCTAATGGCCTACTACACCTTTGTGATCAAATGCTGCTTCAATTGGAACAATATTGATCTTGTTAGAGTAAGACGTTTTTCTTTTTCAAGACAACTTGAAGAACAATCTACGTCGTACTCAATGACATCGGAGCATAGAGGATTAGAACAATCGGTGATTAATTCAATTCCAGTGATTCATTACAAAGAAGAGAAAGACTATGGTGAATTAGGAATCTCTAGTGAATGTGCTTTTTGTTTGAGTGAGTTTCAAGAAGATGAGATACTAAGAGTTATACCAAATTGCAACCATTTATTTCACATTGATTGTGTTGATGTTTGGCTTCAAAACAATGCAAATTGTCCACTTTGTAGAAGAAAAGTTTCAATGACAAGAGAAATCCAAGTTGAGCATGTTGTCACTCCAAGACCTTCACCTTATTTGCAAAGCCAAAATGTTGAAAACATCATTGGTGGTTGTGAAGATTTTGTTGTAATTGATTTGGATAATAATGAAAATGACCATGAAGGTCAAAACTTGCATGAAAGAAGAGATGAAAGAGGTAAAGAATTGGAGGTACCAAGAGATTCACACAAGAAATCAATTAAGCTTCAAAAAGTGTCTAGCATGGGGGATGAGTGTATTAAAGACAAAGATGATGGTTTTCTAGTTCAACCAATTAGAAGGTCTTTCTCAATGGACTTAGCAATACATAAATCTTTATATCAACCTAGTTTGCATGTTCATGGGGTTAGTTCCATTGAAGTTTGTGGTGATAGTAGTAATAGACCTAAGAGATCATTCTTTTCCTTTGGACATGGAAGTAGATCAAGAAGTGTTGTTTTACCAATTCATTTGGACCCTTGA
Protein sequence

>Msa1432140

MGFVNKQHLINHVSQAHPPTQNHLDCGTNFPIVIIVVGMMATFFLLMAYYTFVIKCCFNWNNIDLVRVRRFSFSRQLEEQSTSYSMTSEHRGLEQSVINSIPVIHYKEEKDYGELGISSECAFCLSEFQEDEILRVIPNCNHLFHIDCVDVWLQNNANCPLCRRKVSMTREIQVEHVVTPRPSPYLQSQNVENIIGGCEDFVVIDLDNNENDHEGQNLHERRDERGKELEVPRDSHKKSIKLQKVSSMGDECIKDKDDGFLVQPIRRSFSMDLAIHKSLYQPSLHVHGVSSIEVCGDSSNRPKRSFFSFGHGSRSRSVVLPIHLDP*