AlfalfaGEDB | Msa1443950

M. sativa cultivar zhongmu-4 / Msa1443950

KEGG: K08332
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
PFAM: PF00514.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1443950	sp\|O22193\|PUB4_ARATH	32.857	280	180	5	41	318	543	816	5.82e-34	137

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1443950	A0A072UZV6	98.734	395	5	0	1	395	1	395	0.0	786

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0461980	Msa1443950	0.990870	9.822402e-185	-8.615850e-47
Msa0481960	Msa1443950	0.916293	2.120927e-85	-8.615850e-47
Msa0483330	Msa1443950	0.843143	1.679076e-58	-8.615850e-47
Msa0507420	Msa1443950	0.987285	1.042446e-169	-8.615850e-47
Msa1377300	Msa1443950	0.925829	1.078194e-90	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1443950	MtrunA17_Chr3g0111451	98.734	395	5	0	1	395	1	395	0.0	786
Msa1443950	MtrunA17_Chr5g0435041	74.176	364	93	1	33	395	12	375	4.16e-180	505
Msa1443950	MtrunA17_Chr5g0429231	45.151	299	161	2	48	344	41	338	6.60e-72	229
Msa1443950	MtrunA17_Chr6g0449461	35.913	323	190	8	48	360	9	324	1.00e-45	160
Msa1443950	MtrunA17_Chr3g0134761	33.133	332	203	8	5	325	10	333	5.84e-35	131
Msa1443950	MtrunA17_Chr1g0152561	31.692	325	212	6	3	325	23	339	3.05e-32	124
Msa1443950	MtrunA17_Chr7g0261371	32.759	290	187	5	31	318	504	787	2.32e-31	126
Msa1443950	MtrunA17_Chr3g0106931	32.857	280	180	5	41	318	553	826	3.76e-31	125
Msa1443950	MtrunA17_Chr3g0137061	30.286	350	208	9	56	375	126	469	8.21e-30	120
Msa1443950	MtrunA17_Chr4g0031431	33.333	273	175	5	45	315	357	624	4.50e-29	119

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1443950	AT3G03440.1	70.920	337	96	2	41	375	66	402	1.26e-147	424
Msa1443950	AT4G12710.1	47.164	335	169	5	51	378	57	390	6.41e-80	251
Msa1443950	AT5G14510.1	36.760	321	188	8	48	360	9	322	1.71e-42	152
Msa1443950	AT2G23140.3	32.857	280	180	5	41	318	354	627	2.82e-35	137
Msa1443950	AT2G23140.5	32.857	280	180	5	41	318	354	627	2.82e-35	137
Msa1443950	AT2G23140.2	32.857	280	180	5	41	318	543	816	5.92e-35	137
Msa1443950	AT2G23140.4	32.857	280	180	5	41	318	543	816	5.92e-35	137
Msa1443950	AT2G23140.1	32.857	280	180	5	41	318	546	819	6.05e-35	137
Msa1443950	AT3G01400.1	34.815	270	166	6	58	325	82	343	3.05e-33	127
Msa1443950	AT5G67340.1	33.808	281	177	6	41	318	423	697	8.49e-31	124

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Find 128 sgRNAs with CRISPR-Local

Find 334 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGGCACTCTCCAAAGT+AGG	0.483193	tig0030624:+5540	Msa1443950:CDS
AAAATCAATATTGTGGAGGC+TGG	0.467906	tig0030624:+5110	Msa1443950:CDS
AAAATTGCTTACAATAGTAA+AGG	0.514770	tig0030624:+6192	Msa1443950:three_prime_UTR
AAACCAATTATTAGTGGTTG+TGG	0.468936	tig0030624:+5233	Msa1443950:CDS
AAACGGCACTCTCCAAAGTA+GGG	0.624721	tig0030624:+5541	Msa1443950:CDS
AAAGAAAATGCTAGCTGAGA+TGG	0.495708	tig0030624:+5826	Msa1443950:CDS
AAAGTGATCGATGTAAATAC+AGG	0.437224	tig0030624:+5597	Msa1443950:CDS
AAATACATAAAGTCATATGG+GGG	0.701924	tig0030624:-2083	Msa1443950:intergenic
AACATCATATCTCAAATTGA+CGG	0.612026	tig0030624:+5782	Msa1443950:CDS
AACTTACGAAAACATCACAG+AGG	0.790561	tig0030624:+2416	Msa1443950:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTGTATAGATTATTGGTAG+TGG	+	tig0030624:2637-2656	Msa1443950:intron	30.0%
AAAGAAAAAACGCTATGCTT+AGG	-	tig0030624:2897-2916	Msa1443950:intergenic	30.0%
TTGCATAATCAATTCCTTTC+TGG	+	tig0030624:3016-3035	Msa1443950:intron	30.0%
TTGCATACAAGTAGCATATA+CGG	+	tig0030624:3043-3062	Msa1443950:intron	30.0%
CTTAACAAACTATCTAGTCT+AGG	-	tig0030624:3265-3284	Msa1443950:intergenic	30.0%
TAAGCAAAGTCATGAAAAAG+TGG	-	tig0030624:3355-3374	Msa1443950:intergenic	30.0%
ACAGAAAAACACCAATTTCA+TGG	-	tig0030624:3438-3457	Msa1443950:intergenic	30.0%
TCCTCACCATGAAAAATAAA+AGG	-	tig0030624:3469-3488	Msa1443950:intergenic	30.0%
AAGTTTATCCAATTGGATAG+TGG	+	tig0030624:3637-3656	Msa1443950:intron	30.0%
AAGAAAATCCACTATCCAAT+TGG	-	tig0030624:3648-3667	Msa1443950:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0030624	gene	2027	6229	2027	ID=Msa1443950;Name=Msa1443950
tig0030624	mRNA	2027	6229	2027	ID=Msa1443950-mRNA-1;Parent=Msa1443950;Name=Msa1443950-mRNA-1;_AED=0.00;_eAED=0.00;_QI=196\|1\|1\|1\|1\|1\|2\|271\|395
tig0030624	exon	2027	2557	2027	ID=Msa1443950-mRNA-1:exon:14191;Parent=Msa1443950-mRNA-1
tig0030624	exon	5106	6229	5106	ID=Msa1443950-mRNA-1:exon:14192;Parent=Msa1443950-mRNA-1
tig0030624	five_prime_UTR	2027	2222	2027	ID=Msa1443950-mRNA-1:five_prime_utr;Parent=Msa1443950-mRNA-1
tig0030624	CDS	2223	2557	2223	ID=Msa1443950-mRNA-1:cds;Parent=Msa1443950-mRNA-1
tig0030624	CDS	5106	5958	5106	ID=Msa1443950-mRNA-1:cds;Parent=Msa1443950-mRNA-1
tig0030624	three_prime_UTR	5959	6229	5959	ID=Msa1443950-mRNA-1:three_prime_utr;Parent=Msa1443950-mRNA-1

Gene Sequence

>Msa1443950

ATGGACACTCCTCATTCACCTTCCTCCTGTTCCTCCTCCTCCTCCAATTATTCATGGGAAGGAGGGCATCCTACCGACACAATTATTATAGAAGCTGAAGAGCCGCAGACCCCATTAGCCGTCCGTCGTGCTCTTCAGCTTCTTAAATCCGGCGTGCCGGAGTTGCGGCTTCAAGCCGCACGTGATATTAGGAAACTTACGAAAACATCACAGAGGTGTCGGCGTCAGTTGTCTGAAGCTGTCGGTACGCTTGTTTCTATGCTCCGAGTCGACTCGGTTGAGTCTCATGAACCGGCTCTTCTTGCTTTGCTTAATCTCGCCGTCAAAGATGAAAAGAATAAAATCAATATTGTGGAGGCTGGAGCATTAGAACCAATACTTAGTTTCCTCAAGTCAGAAAATCTAAATCTACAGGCATCTGCAGCTGCAGCTTTGCTCACACTATCTGCCTCTTCAATCAACAAACCAATTATTAGTGGTTGTGGCGCCATTCCCCTTCTTGTCGAGATTCTTAGAGACGGAAGCCCACAGGCGAAAGCTGATGCAGTAATGGCTCTCTCTAATTTATCAACATACCCTAATAATCTTAGCCTTATTCTCGAAACAAATCCAATCCCTCACATAGTTGATATCCTCAAAACATGCAAAAAGTCCTCAAAAACAGCTGAAAAATGCTGTGCTTTGATAGAACCCTTGGTGGACTATGATGAATGCAGAACTGCATTGACATCCGAGGAAGGTGGTATTCTTGCAGTCGTGGAGGTTCTTGAAAACGGCACTCTCCAAAGTAGGGAACATGCTGTTGGAACACTATTGACAATGTGCCAAAGTGATCGATGTAAATACAGGGAACCGATCCTTAGAGAAGGTGTCATTCCAGGACTACTTGAGCTCACTGTACAAGGAACACCGAAGTCTCAGACAAAAGCGCGCACCCTTTTGCAACTACTAAGAGAATCTCCTTATCCAAGATCTGAAATCCAACCCGACACTCTTGAGAATATAGTATGCAACATCATATCTCAAATTGACGGGGATGATCAATCTGGTAAAGCAAAGAAAATGCTAGCTGAGATGGTTCAAGTCAGCATGGAACAAAGTCTGAGGCATTTACAGCAAAGGGCACTTGTATGCACCCCAAGTGATCTCCCTATAGCTGGCTGTGCTTCTGAAGTTTCTTTTAAGTAA

Protein sequence

>Msa1443950

MDTPHSPSSCSSSSSNYSWEGGHPTDTIIIEAEEPQTPLAVRRALQLLKSGVPELRLQAARDIRKLTKTSQRCRRQLSEAVGTLVSMLRVDSVESHEPALLALLNLAVKDEKNKINIVEAGALEPILSFLKSENLNLQASAAAALLTLSASSINKPIISGCGAIPLLVEILRDGSPQAKADAVMALSNLSTYPNNLSLILETNPIPHIVDILKTCKKSSKTAEKCCALIEPLVDYDECRTALTSEEGGILAVVEVLENGTLQSREHAVGTLLTMCQSDRCKYREPILREGVIPGLLELTVQGTPKSQTKARTLLQLLRESPYPRSEIQPDTLENIVCNIISQIDGDDQSGKAKKMLAEMVQVSMEQSLRHLQQRALVCTPSDLPIAGCASEVSFK*