AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1444680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1444680 MtrunA17_Chr8g0336121 100.000 92 0 0 1 92 1 92 7.50e-63 185
Msa1444680 MtrunA17_Chr4g0055341 100.000 92 0 0 1 92 1 92 7.50e-63 185
Msa1444680 MtrunA17_Chr4g0057431 100.000 92 0 0 1 92 1 92 7.50e-63 185
Msa1444680 MtrunA17_Chr8g0336341 100.000 92 0 0 1 92 1 92 7.50e-63 185
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1444680 AT3G10950.1 93.478 92 6 0 1 92 1 92 4.50e-59 176
Msa1444680 AT3G60245.1 92.308 91 7 0 1 91 1 91 8.73e-58 172

Find 40 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGTACATTATAATATTT+TGG 0.135695 tig0030801:+8684 Msa1444680:three_prime_UTR
AAGCATTGAAAGAATCTATT+AGG 0.280966 tig0030801:-8586 None:intergenic
AGAGAAAGGCTGTAGGAATA+TGG 0.316747 tig0030801:+8177 Msa1444680:CDS
GCTTCTTCTTAAATTAAGTT+TGG 0.336508 tig0030801:+8419 Msa1444680:three_prime_UTR
TAGCTCTGCACAATCAATTA+AGG 0.386874 tig0030801:+8811 Msa1444680:three_prime_UTR
GAGAAAGGCTGTAGGAATAT+GGG 0.416937 tig0030801:+8178 Msa1444680:CDS
GAACAATCATGGTTCTGCTT+TGG 0.422461 tig0030801:+8772 Msa1444680:three_prime_UTR
GCTGGCATCGTTGGCAAATA+CGG 0.428463 tig0030801:+7383 Msa1444680:CDS
ATGATATTAGGTACCCGTTA+TGG 0.441488 tig0030801:+7954 Msa1444680:intron
CTGCGTCTGCTGTGACTGTT+CGG 0.466153 tig0030801:+8337 Msa1444680:CDS
TTGCTCCCTCAGCCTCCTGA+TGG 0.474414 tig0030801:-8364 None:intergenic
ACGCAAACTAGCACCATAAC+GGG 0.476774 tig0030801:-7967 None:intergenic
TGACTGTTCGGAGCACCATC+AGG 0.483900 tig0030801:+8349 Msa1444680:CDS
AGCAGTATTCTATAATCAAA+AGG 0.487147 tig0030801:-8707 None:intergenic
TCGTGATTCTGCTGAGGCCA+TGG 0.495949 tig0030801:+8666 Msa1444680:three_prime_UTR
ACAAAGAAGGCTGGCATCGT+TGG 0.496247 tig0030801:+7374 Msa1444680:CDS
GTGGAGTGGCAGCGGTGGAA+TGG 0.496830 tig0030801:-7168 None:intergenic
ATCGAAGGATGTGTGAGCTA+TGG 0.523123 tig0030801:-7197 None:intergenic
TACGCAAACTAGCACCATAA+CGG 0.535734 tig0030801:-7968 None:intergenic
AGAAAGGCTGTAGGAATATG+GGG 0.537082 tig0030801:+8179 Msa1444680:CDS
GGAGCACCATCAGGAGGCTG+AGG 0.547327 tig0030801:+8358 Msa1444680:CDS
GTGGAGTGGAGTGGCAGCGG+TGG 0.554478 tig0030801:-7173 None:intergenic
ACTGCTTAGTAGAACAATCA+TGG 0.557531 tig0030801:+8761 Msa1444680:three_prime_UTR
GACTGCGGTAAAGTGAAAGC+AGG 0.566191 tig0030801:+8209 Msa1444680:CDS
AGGTTGTTGCAGATAATGAA+AGG 0.567069 tig0030801:-8566 None:intergenic
ACTAAGAGAACAAAGAAGGC+TGG 0.571248 tig0030801:+7365 Msa1444680:CDS
GGAGTGGAGTGGAGTGGCAG+CGG 0.573220 tig0030801:-7176 None:intergenic
TGTAGGAATATGGGGATGCA+AGG 0.596749 tig0030801:+8187 Msa1444680:CDS
GCAGACTAAGAGAACAAAGA+AGG 0.598781 tig0030801:+7361 Msa1444680:intron
CAAAATATTATAATGTACCA+TGG 0.617361 tig0030801:-8683 None:intergenic
GTGTGAGCTATGGAGTGGAG+TGG 0.620732 tig0030801:-7187 None:intergenic
GAGCACCATCAGGAGGCTGA+GGG 0.623110 tig0030801:+8359 Msa1444680:CDS
GCAGTATGCTGTGAAGAGAA+AGG 0.632531 tig0030801:+8163 Msa1444680:intron
TGCGGTAAAGTGAAAGCAGG+CGG 0.647382 tig0030801:+8212 Msa1444680:CDS
CTGTTCGGAGCACCATCAGG+AGG 0.656338 tig0030801:+8352 Msa1444680:CDS
GCTGTGAAGAGAAAGGCTGT+AGG 0.663437 tig0030801:+8170 Msa1444680:CDS
AGCTATGGAGTGGAGTGGAG+TGG 0.679244 tig0030801:-7182 None:intergenic
ATAATGAAAGGAAACAACAA+AGG 0.683390 tig0030801:-8554 None:intergenic
ATATGGGGATGCAAGGACTG+CGG 0.722475 tig0030801:+8194 Msa1444680:CDS
AGGATGTGTGAGCTATGGAG+TGG 0.727679 tig0030801:-7192 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTATTGTATGATATT+AGG + tig0030801:7942-7961 Msa1444680:intron 10.0%
!!! TTATTTTATTGTATGATATT+AGG + tig0030801:7942-7961 Msa1444680:intron 10.0%
!!! AATTTTCTTATTCTTTTTCT+CGG + tig0030801:8253-8272 Msa1444680:intron 15.0%
!!! TTTTATTTTTCTATTCTGAA+AGG + tig0030801:8278-8297 Msa1444680:intron 15.0%
!!! AATTTTCTTATTCTTTTTCT+CGG + tig0030801:8253-8272 Msa1444680:intron 15.0%
!!! TTTTATTTTTCTATTCTGAA+AGG + tig0030801:8278-8297 Msa1444680:intron 15.0%
!!! TTGAAAGAAATGTTTAGAAT+TGG - tig0030801:7267-7286 None:intergenic 20.0%
!! ATCTAAATAATTATGCACAT+AGG + tig0030801:7525-7544 Msa1444680:intron 20.0%
!! TTATTTATTTGCATAAGCTA+AGG - tig0030801:7767-7786 None:intergenic 20.0%
!!! AATAAATAACCTTTTGTGTA+AGG + tig0030801:7674-7693 Msa1444680:intron 20.0%
!!! AAATTTTTCTGTGAATTTTG+TGG + tig0030801:8026-8045 Msa1444680:CDS 20.0%
!!! ATCAAATCATTTAGTTTACT+AGG + tig0030801:8090-8109 Msa1444680:intron 20.0%
!! CAAAATATTATAATGTACCA+TGG - tig0030801:8686-8705 None:intergenic 20.0%
!!! CATGGTACATTATAATATTT+TGG + tig0030801:8684-8703 Msa1444680:three_prime_UTR 20.0%
!!! CTTAATATAACGATTTTGAA+GGG + tig0030801:8728-8747 Msa1444680:three_prime_UTR 20.0%
!!! TTGAAAGAAATGTTTAGAAT+TGG - tig0030801:7267-7286 None:intergenic 20.0%
!! ATCTAAATAATTATGCACAT+AGG + tig0030801:7525-7544 Msa1444680:intron 20.0%
!! TTATTTATTTGCATAAGCTA+AGG - tig0030801:7767-7786 None:intergenic 20.0%
!!! AATAAATAACCTTTTGTGTA+AGG + tig0030801:7674-7693 Msa1444680:intron 20.0%
!!! AAATTTTTCTGTGAATTTTG+TGG + tig0030801:8026-8045 Msa1444680:CDS 20.0%
!!! ATCAAATCATTTAGTTTACT+AGG + tig0030801:8090-8109 Msa1444680:intron 20.0%
!! CAAAATATTATAATGTACCA+TGG - tig0030801:8686-8705 None:intergenic 20.0%
!!! CATGGTACATTATAATATTT+TGG + tig0030801:8684-8703 Msa1444680:three_prime_UTR 20.0%
!!! CTTAATATAACGATTTTGAA+GGG + tig0030801:8728-8747 Msa1444680:three_prime_UTR 20.0%
!! AGAAATGTTTAGAATTGGAT+TGG - tig0030801:7262-7281 None:intergenic 25.0%
! AAGTACAGATTCAACATAAA+AGG - tig0030801:7326-7345 None:intergenic 25.0%
!! AAATGTGGTTTAGTTTATGA+TGG + tig0030801:7467-7486 Msa1444680:intron 25.0%
! ATAATTATGCACATAGGTAT+AGG + tig0030801:7531-7550 Msa1444680:intron 25.0%
!! TTCTAGGTGGTTTAATATTT+TGG + tig0030801:7827-7846 Msa1444680:intron 25.0%
!! TCTAGGTGGTTTAATATTTT+GGG + tig0030801:7828-7847 Msa1444680:intron 25.0%
! ACAAATACAGTTCATGAATT+GGG - tig0030801:7880-7899 None:intergenic 25.0%
! TACAAATACAGTTCATGAAT+TGG - tig0030801:7881-7900 None:intergenic 25.0%
! ATAATGATAACAAAGTCATG+AGG - tig0030801:8307-8326 None:intergenic 25.0%
! GCTTCTTCTTAAATTAAGTT+TGG + tig0030801:8419-8438 Msa1444680:three_prime_UTR 25.0%
! ATAATGAAAGGAAACAACAA+AGG - tig0030801:8557-8576 None:intergenic 25.0%
!!! AAGCATTGAAAGAATCTATT+AGG - tig0030801:8589-8608 None:intergenic 25.0%
! ATCAAAACAAGAAAAACACT+AGG - tig0030801:8632-8651 None:intergenic 25.0%
! AGCAGTATTCTATAATCAAA+AGG - tig0030801:8710-8729 None:intergenic 25.0%
!! GCTTAATATAACGATTTTGA+AGG + tig0030801:8727-8746 Msa1444680:three_prime_UTR 25.0%
!! AGAAATGTTTAGAATTGGAT+TGG - tig0030801:7262-7281 None:intergenic 25.0%
! AAGTACAGATTCAACATAAA+AGG - tig0030801:7326-7345 None:intergenic 25.0%
!! AAATGTGGTTTAGTTTATGA+TGG + tig0030801:7467-7486 Msa1444680:intron 25.0%
! ATAATTATGCACATAGGTAT+AGG + tig0030801:7531-7550 Msa1444680:intron 25.0%
!! TTCTAGGTGGTTTAATATTT+TGG + tig0030801:7827-7846 Msa1444680:intron 25.0%
!! TCTAGGTGGTTTAATATTTT+GGG + tig0030801:7828-7847 Msa1444680:intron 25.0%
! ACAAATACAGTTCATGAATT+GGG - tig0030801:7880-7899 None:intergenic 25.0%
! TACAAATACAGTTCATGAAT+TGG - tig0030801:7881-7900 None:intergenic 25.0%
! ATAATGATAACAAAGTCATG+AGG - tig0030801:8307-8326 None:intergenic 25.0%
! GCTTCTTCTTAAATTAAGTT+TGG + tig0030801:8419-8438 Msa1444680:three_prime_UTR 25.0%
! ATAATGAAAGGAAACAACAA+AGG - tig0030801:8557-8576 None:intergenic 25.0%
!!! AAGCATTGAAAGAATCTATT+AGG - tig0030801:8589-8608 None:intergenic 25.0%
! ATCAAAACAAGAAAAACACT+AGG - tig0030801:8632-8651 None:intergenic 25.0%
! AGCAGTATTCTATAATCAAA+AGG - tig0030801:8710-8729 None:intergenic 25.0%
!! GCTTAATATAACGATTTTGA+AGG + tig0030801:8727-8746 Msa1444680:three_prime_UTR 25.0%
! TTTAGAATTGGATTGGATGA+TGG - tig0030801:7255-7274 None:intergenic 30.0%
GTTCTTCTATGTTGTAAATG+TGG + tig0030801:7452-7471 Msa1444680:intron 30.0%
AAGGAATAAACCAATCGAAA+TGG - tig0030801:7644-7663 None:intergenic 30.0%
! CTAGGTGGTTTAATATTTTG+GGG + tig0030801:7829-7848 Msa1444680:intron 30.0%
! TTTCTGTGAATTTTGTGGAA+AGG + tig0030801:8031-8050 Msa1444680:CDS 30.0%
!! CAGAACTCAACATTTTGAAA+AGG - tig0030801:8126-8145 None:intergenic 30.0%
! TTTAGAATTGGATTGGATGA+TGG - tig0030801:7255-7274 None:intergenic 30.0%
GTTCTTCTATGTTGTAAATG+TGG + tig0030801:7452-7471 Msa1444680:intron 30.0%
AAGGAATAAACCAATCGAAA+TGG - tig0030801:7644-7663 None:intergenic 30.0%
! CTAGGTGGTTTAATATTTTG+GGG + tig0030801:7829-7848 Msa1444680:intron 30.0%
! TTTCTGTGAATTTTGTGGAA+AGG + tig0030801:8031-8050 Msa1444680:CDS 30.0%
!! CAGAACTCAACATTTTGAAA+AGG - tig0030801:8126-8145 None:intergenic 30.0%
TCCTTCGATCTCTGTAAAAA+TGG + tig0030801:7211-7230 Msa1444680:exon 35.0%
!! ACCATTTTTACAGAGATCGA+AGG - tig0030801:7215-7234 None:intergenic 35.0%
TGCACATAGGTATAGGTATA+TGG + tig0030801:7538-7557 Msa1444680:intron 35.0%
AACAACAGTTAATGTCACGT+GGG - tig0030801:7603-7622 None:intergenic 35.0%
CATACACTACCTTACACAAA+AGG - tig0030801:7686-7705 None:intergenic 35.0%
ACCTAGAATGTAACTACCTA+GGG - tig0030801:7815-7834 None:intergenic 35.0%
TGAACTGTATTTGTAGAGCA+AGG + tig0030801:7886-7905 Msa1444680:intron 35.0%
ATGATATTAGGTACCCGTTA+TGG + tig0030801:7954-7973 Msa1444680:intron 35.0%
GCGTAAGCAGATTAAAAAGA+TGG + tig0030801:7986-8005 Msa1444680:CDS 35.0%
AGGTTGTTGCAGATAATGAA+AGG - tig0030801:8569-8588 None:intergenic 35.0%
! TTTTATTCGTGATTCTGCTG+AGG + tig0030801:8660-8679 Msa1444680:three_prime_UTR 35.0%
ACTGCTTAGTAGAACAATCA+TGG + tig0030801:8761-8780 Msa1444680:three_prime_UTR 35.0%
! TAGCTCTGCACAATCAATTA+AGG + tig0030801:8811-8830 Msa1444680:three_prime_UTR 35.0%
TCCTTCGATCTCTGTAAAAA+TGG + tig0030801:7211-7230 Msa1444680:exon 35.0%
!! ACCATTTTTACAGAGATCGA+AGG - tig0030801:7215-7234 None:intergenic 35.0%
TGCACATAGGTATAGGTATA+TGG + tig0030801:7538-7557 Msa1444680:intron 35.0%
AACAACAGTTAATGTCACGT+GGG - tig0030801:7603-7622 None:intergenic 35.0%
CATACACTACCTTACACAAA+AGG - tig0030801:7686-7705 None:intergenic 35.0%
ACCTAGAATGTAACTACCTA+GGG - tig0030801:7815-7834 None:intergenic 35.0%
TGAACTGTATTTGTAGAGCA+AGG + tig0030801:7886-7905 Msa1444680:intron 35.0%
ATGATATTAGGTACCCGTTA+TGG + tig0030801:7954-7973 Msa1444680:intron 35.0%
GCGTAAGCAGATTAAAAAGA+TGG + tig0030801:7986-8005 Msa1444680:CDS 35.0%
AGGTTGTTGCAGATAATGAA+AGG - tig0030801:8569-8588 None:intergenic 35.0%
! TTTTATTCGTGATTCTGCTG+AGG + tig0030801:8660-8679 Msa1444680:three_prime_UTR 35.0%
ACTGCTTAGTAGAACAATCA+TGG + tig0030801:8761-8780 Msa1444680:three_prime_UTR 35.0%
!! ATTGGATTGGATGATGGAGA+AGG - tig0030801:7249-7268 None:intergenic 40.0%
GCAGACTAAGAGAACAAAGA+AGG + tig0030801:7361-7380 Msa1444680:intron 40.0%
ACTAAGAGAACAAAGAAGGC+TGG + tig0030801:7365-7384 Msa1444680:CDS 40.0%
CAACAACAGTTAATGTCACG+TGG - tig0030801:7604-7623 None:intergenic 40.0%
TGTATAGCGACCATTTCGAT+TGG + tig0030801:7631-7650 Msa1444680:intron 40.0%
CTTCATAAGCCAATCCAAAC+AGG + tig0030801:7789-7808 Msa1444680:intron 40.0%
CACCTAGAATGTAACTACCT+AGG - tig0030801:7816-7835 None:intergenic 40.0%
CTAGGTAGTTACATTCTAGG+TGG + tig0030801:7814-7833 Msa1444680:intron 40.0%
TACGCAAACTAGCACCATAA+CGG - tig0030801:7971-7990 None:intergenic 40.0%
AGAGAAAGGCTGTAGGAATA+TGG + tig0030801:8177-8196 Msa1444680:CDS 40.0%
GAGAAAGGCTGTAGGAATAT+GGG + tig0030801:8178-8197 Msa1444680:CDS 40.0%
AGAAAGGCTGTAGGAATATG+GGG + tig0030801:8179-8198 Msa1444680:CDS 40.0%
! GAACAATCATGGTTCTGCTT+TGG + tig0030801:8772-8791 Msa1444680:three_prime_UTR 40.0%
!! ATTGGATTGGATGATGGAGA+AGG - tig0030801:7249-7268 None:intergenic 40.0%
GCAGACTAAGAGAACAAAGA+AGG + tig0030801:7361-7380 Msa1444680:intron 40.0%
ACTAAGAGAACAAAGAAGGC+TGG + tig0030801:7365-7384 Msa1444680:CDS 40.0%
CAACAACAGTTAATGTCACG+TGG - tig0030801:7604-7623 None:intergenic 40.0%
TGTATAGCGACCATTTCGAT+TGG + tig0030801:7631-7650 Msa1444680:intron 40.0%
CTTCATAAGCCAATCCAAAC+AGG + tig0030801:7789-7808 Msa1444680:intron 40.0%
CACCTAGAATGTAACTACCT+AGG - tig0030801:7816-7835 None:intergenic 40.0%
CTAGGTAGTTACATTCTAGG+TGG + tig0030801:7814-7833 Msa1444680:intron 40.0%
TACGCAAACTAGCACCATAA+CGG - tig0030801:7971-7990 None:intergenic 40.0%
AGAGAAAGGCTGTAGGAATA+TGG + tig0030801:8177-8196 Msa1444680:CDS 40.0%
GAGAAAGGCTGTAGGAATAT+GGG + tig0030801:8178-8197 Msa1444680:CDS 40.0%
AGAAAGGCTGTAGGAATATG+GGG + tig0030801:8179-8198 Msa1444680:CDS 40.0%
! GAACAATCATGGTTCTGCTT+TGG + tig0030801:8772-8791 Msa1444680:three_prime_UTR 40.0%
ATCGAAGGATGTGTGAGCTA+TGG - tig0030801:7200-7219 None:intergenic 45.0%
CAGTTAATGTCACGTGGGTT+CGG - tig0030801:7598-7617 None:intergenic 45.0%
GCCCTAGGTAGTTACATTCT+AGG + tig0030801:7811-7830 Msa1444680:intron 45.0%
ACGCAAACTAGCACCATAAC+GGG - tig0030801:7970-7989 None:intergenic 45.0%
GCAGTATGCTGTGAAGAGAA+AGG + tig0030801:8163-8182 Msa1444680:intron 45.0%
TGTAGGAATATGGGGATGCA+AGG + tig0030801:8187-8206 Msa1444680:CDS 45.0%
ATCGAAGGATGTGTGAGCTA+TGG - tig0030801:7200-7219 None:intergenic 45.0%
CAGTTAATGTCACGTGGGTT+CGG - tig0030801:7598-7617 None:intergenic 45.0%
GCCCTAGGTAGTTACATTCT+AGG + tig0030801:7811-7830 Msa1444680:intron 45.0%
ACGCAAACTAGCACCATAAC+GGG - tig0030801:7970-7989 None:intergenic 45.0%
GCAGTATGCTGTGAAGAGAA+AGG + tig0030801:8163-8182 Msa1444680:intron 45.0%
TGTAGGAATATGGGGATGCA+AGG + tig0030801:8187-8206 Msa1444680:CDS 45.0%
AGGATGTGTGAGCTATGGAG+TGG - tig0030801:7195-7214 None:intergenic 50.0%
ACAAAGAAGGCTGGCATCGT+TGG + tig0030801:7374-7393 Msa1444680:CDS 50.0%
GCTGGCATCGTTGGCAAATA+CGG + tig0030801:7383-7402 Msa1444680:CDS 50.0%
TACCTAGGGCCTGTTTGGAT+TGG - tig0030801:7801-7820 None:intergenic 50.0%
GTAACTACCTAGGGCCTGTT+TGG - tig0030801:7806-7825 None:intergenic 50.0%
GCTGTGAAGAGAAAGGCTGT+AGG + tig0030801:8170-8189 Msa1444680:CDS 50.0%
ATATGGGGATGCAAGGACTG+CGG + tig0030801:8194-8213 Msa1444680:CDS 50.0%
GACTGCGGTAAAGTGAAAGC+AGG + tig0030801:8209-8228 Msa1444680:CDS 50.0%
TGCGGTAAAGTGAAAGCAGG+CGG + tig0030801:8212-8231 Msa1444680:CDS 50.0%
AGGATGTGTGAGCTATGGAG+TGG - tig0030801:7195-7214 None:intergenic 50.0%
ACAAAGAAGGCTGGCATCGT+TGG + tig0030801:7374-7393 Msa1444680:CDS 50.0%
GCTGGCATCGTTGGCAAATA+CGG + tig0030801:7383-7402 Msa1444680:CDS 50.0%
TACCTAGGGCCTGTTTGGAT+TGG - tig0030801:7801-7820 None:intergenic 50.0%
GTAACTACCTAGGGCCTGTT+TGG - tig0030801:7806-7825 None:intergenic 50.0%
GCTGTGAAGAGAAAGGCTGT+AGG + tig0030801:8170-8189 Msa1444680:CDS 50.0%
ATATGGGGATGCAAGGACTG+CGG + tig0030801:8194-8213 Msa1444680:CDS 50.0%
GACTGCGGTAAAGTGAAAGC+AGG + tig0030801:8209-8228 Msa1444680:CDS 50.0%
TGCGGTAAAGTGAAAGCAGG+CGG + tig0030801:8212-8231 Msa1444680:CDS 50.0%
AGCTATGGAGTGGAGTGGAG+TGG - tig0030801:7185-7204 None:intergenic 55.0%
GTGTGAGCTATGGAGTGGAG+TGG - tig0030801:7190-7209 None:intergenic 55.0%
AGCCAATCCAAACAGGCCCT+AGG + tig0030801:7796-7815 Msa1444680:intron 55.0%
CTGCGTCTGCTGTGACTGTT+CGG + tig0030801:8337-8356 Msa1444680:CDS 55.0%
! TGACTGTTCGGAGCACCATC+AGG + tig0030801:8349-8368 Msa1444680:CDS 55.0%
TCGTGATTCTGCTGAGGCCA+TGG + tig0030801:8666-8685 Msa1444680:three_prime_UTR 55.0%
AGCTATGGAGTGGAGTGGAG+TGG - tig0030801:7185-7204 None:intergenic 55.0%
GTGTGAGCTATGGAGTGGAG+TGG - tig0030801:7190-7209 None:intergenic 55.0%
AGCCAATCCAAACAGGCCCT+AGG + tig0030801:7796-7815 Msa1444680:intron 55.0%
CTGCGTCTGCTGTGACTGTT+CGG + tig0030801:8337-8356 Msa1444680:CDS 55.0%
! TGACTGTTCGGAGCACCATC+AGG + tig0030801:8349-8368 Msa1444680:CDS 55.0%
TCGTGATTCTGCTGAGGCCA+TGG + tig0030801:8666-8685 Msa1444680:three_prime_UTR 55.0%
! CTGTTCGGAGCACCATCAGG+AGG + tig0030801:8352-8371 Msa1444680:CDS 60.0%
GAGCACCATCAGGAGGCTGA+GGG + tig0030801:8359-8378 Msa1444680:CDS 60.0%
TTGCTCCCTCAGCCTCCTGA+TGG - tig0030801:8367-8386 None:intergenic 60.0%
! CTGTTCGGAGCACCATCAGG+AGG + tig0030801:8352-8371 Msa1444680:CDS 60.0%
GAGCACCATCAGGAGGCTGA+GGG + tig0030801:8359-8378 Msa1444680:CDS 60.0%
TTGCTCCCTCAGCCTCCTGA+TGG - tig0030801:8367-8386 None:intergenic 60.0%
GTGGAGTGGCAGCGGTGGAA+TGG - tig0030801:7171-7190 None:intergenic 65.0%
GGAGTGGAGTGGAGTGGCAG+CGG - tig0030801:7179-7198 None:intergenic 65.0%
! GGAGCACCATCAGGAGGCTG+AGG + tig0030801:8358-8377 Msa1444680:CDS 65.0%
GTGGAGTGGCAGCGGTGGAA+TGG - tig0030801:7171-7190 None:intergenic 65.0%
GGAGTGGAGTGGAGTGGCAG+CGG - tig0030801:7179-7198 None:intergenic 65.0%
! GGAGCACCATCAGGAGGCTG+AGG + tig0030801:8358-8377 Msa1444680:CDS 65.0%
GTGGAGTGGAGTGGCAGCGG+TGG - tig0030801:7176-7195 None:intergenic 70.0%
GTGGAGTGGAGTGGCAGCGG+TGG - tig0030801:7176-7195 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0030801 gene 7159 8833 7159 ID=Msa1444680;Name=Msa1444680
tig0030801 mRNA 7159 8833 7159 ID=Msa1444680-mRNA-1;Parent=Msa1444680;Name=Msa1444680-mRNA-1;_AED=0.39;_eAED=0.39;_QI=71|1|1|1|1|1|5|435|92
tig0030801 exon 7159 7232 7159 ID=Msa1444680-mRNA-1:exon:14617;Parent=Msa1444680-mRNA-1
tig0030801 exon 7365 7404 7365 ID=Msa1444680-mRNA-1:exon:14618;Parent=Msa1444680-mRNA-1
tig0030801 exon 7964 8052 7964 ID=Msa1444680-mRNA-1:exon:14619;Parent=Msa1444680-mRNA-1
tig0030801 exon 8167 8249 8167 ID=Msa1444680-mRNA-1:exon:14620;Parent=Msa1444680-mRNA-1
tig0030801 exon 8335 8833 8335 ID=Msa1444680-mRNA-1:exon:14621;Parent=Msa1444680-mRNA-1
tig0030801 five_prime_UTR 7159 7229 7159 ID=Msa1444680-mRNA-1:five_prime_utr;Parent=Msa1444680-mRNA-1
tig0030801 CDS 7230 7232 7230 ID=Msa1444680-mRNA-1:cds;Parent=Msa1444680-mRNA-1
tig0030801 CDS 7365 7404 7365 ID=Msa1444680-mRNA-1:cds;Parent=Msa1444680-mRNA-1
tig0030801 CDS 7964 8052 7964 ID=Msa1444680-mRNA-1:cds;Parent=Msa1444680-mRNA-1
tig0030801 CDS 8167 8249 8167 ID=Msa1444680-mRNA-1:cds;Parent=Msa1444680-mRNA-1
tig0030801 CDS 8335 8398 8335 ID=Msa1444680-mRNA-1:cds;Parent=Msa1444680-mRNA-1
tig0030801 three_prime_UTR 8399 8833 8399 ID=Msa1444680-mRNA-1:three_prime_utr;Parent=Msa1444680-mRNA-1
Gene Sequence

>Msa1444680

ATGACTAAGAGAACAAAGAAGGCTGGCATCGTTGGCAAATACGGTACCCGTTATGGTGCTAGTTTGCGTAAGCAGATTAAAAAGATGGAAGTCAGTCAGCACAGTAAATTTTTCTGTGAATTTTGTGGAAAGTATGCTGTGAAGAGAAAGGCTGTAGGAATATGGGGATGCAAGGACTGCGGTAAAGTGAAAGCAGGCGGAGCTTACACTTTGAACACTGCGTCTGCTGTGACTGTTCGGAGCACCATCAGGAGGCTGAGGGAGCAAACTGAAAGTTAA
Protein sequence

>Msa1444680

MTKRTKKAGIVGKYGTRYGASLRKQIKKMEVSQHSKFFCEFCGKYAVKRKAVGIWGCKDCGKVKAGGAYTLNTASAVTVRSTIRRLREQTES*