AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1449030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449030 MtrunA17_Chr7g0237291 94.702 151 8 0 1 151 358 508 3.39e-101 299
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449030 AT2G23470.1 43.056 144 81 1 6 148 372 515 3.72e-34 125

Find 56 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAGAAAAGTAAAGTTTAT+TGG 0.154982 tig0032425:-1713 None:intergenic
TTGTTGGCTCCTTAAAATTA+TGG 0.157375 tig0032425:+1571 Msa1449030:three_prime_UTR
TGAATCAAAGCATATAATTA+TGG 0.225266 tig0032425:+440 Msa1449030:CDS
CTTAAAATTATGGGAGTGTT+TGG 0.252938 tig0032425:+1581 Msa1449030:three_prime_UTR
TTAAAATTATGGGAGTGTTT+GGG 0.258807 tig0032425:+1582 Msa1449030:three_prime_UTR
TTTATGCACCCAATTCTAAT+TGG 0.286530 tig0032425:-171 None:intergenic
AGCTTATGGCCAATTAGAAT+TGG 0.308203 tig0032425:+162 Msa1449030:CDS
TATAATTATGGGTTTGTTGC+AGG 0.310133 tig0032425:+452 Msa1449030:CDS
TGTTGGCTCCTTAAAATTAT+GGG 0.314709 tig0032425:+1572 Msa1449030:three_prime_UTR
AAAAGTAAAGTTTATTGGTT+TGG 0.317795 tig0032425:-1708 None:intergenic
TTGATTCAGATAAAAGCTTA+TGG 0.333751 tig0032425:+148 Msa1449030:intron
TTTGACTTTAACACTCTAGA+TGG 0.334482 tig0032425:-1749 None:intergenic
TCATTGAACACTCAGGTATT+CGG 0.375957 tig0032425:-203 None:intergenic
CTCATCAGTTATGCTTTGTA+TGG 0.378873 tig0032425:-229 None:intergenic
GAATCAAAGCATATAATTAT+GGG 0.387866 tig0032425:+441 Msa1449030:CDS
GGGGTTAACTTTAGCTGAAA+AGG 0.391228 tig0032425:+1602 Msa1449030:three_prime_UTR
CACTCAGGTATTCGGTCCTT+GGG 0.396675 tig0032425:-195 None:intergenic
TCTTTCTCGCTCACATCTGC+CGG 0.408048 tig0032425:-28 None:intergenic
TTTATTGTCAAAGCAAGAAC+AGG 0.414888 tig0032425:+1501 Msa1449030:CDS
ACACTCAGGTATTCGGTCCT+TGG 0.422607 tig0032425:-196 None:intergenic
TGTTTATGATTAATTAAAGA+TGG 0.430642 tig0032425:+1537 Msa1449030:exon
TGGAAAGAGGTATGCAGAAA+GGG 0.441734 tig0032425:+1423 Msa1449030:CDS
ACAAATGGTGGGAATGGGTT+GGG 0.443038 tig0032425:+1465 Msa1449030:CDS
AACTAATTTGATTGAACAAA+TGG 0.443306 tig0032425:+1450 Msa1449030:CDS
CTTTCTCGCTCACATCTGCC+GGG 0.448206 tig0032425:-27 None:intergenic
GCTTTGATTCAGCTCCTTCA+CGG 0.449333 tig0032425:-428 None:intergenic
GCTTATGGCCAATTAGAATT+GGG 0.467619 tig0032425:+163 Msa1449030:CDS
GATTGAACAAATGGTGGGAA+TGG 0.476216 tig0032425:+1459 Msa1449030:CDS
AACAAATGGTGGGAATGGGT+TGG 0.477988 tig0032425:+1464 Msa1449030:CDS
CAAACTCAGCACTTGAGGAT+TGG 0.487473 tig0032425:+1383 Msa1449030:CDS
ATGGAAAGAGGTATGCAGAA+AGG 0.493829 tig0032425:+1422 Msa1449030:CDS
TAAAGTGGAACATTAGCATA+GGG 0.494438 tig0032425:+1347 Msa1449030:CDS
GGATTATTACTTTGTATGCA+TGG 0.502779 tig0032425:+251 Msa1449030:CDS
CTCCTTCACGGATGGAAAGA+AGG 0.503738 tig0032425:-416 None:intergenic
ATTGAACAAATGGTGGGAAT+GGG 0.515576 tig0032425:+1460 Msa1449030:CDS
TCAGAAGGACTCTTCTAAAG+TGG 0.516087 tig0032425:+1332 Msa1449030:CDS
TGGTCTGTAATTGTTGAGGA+TGG 0.520551 tig0032425:+1403 Msa1449030:CDS
TGATTCAGCTCCTTCACGGA+TGG 0.533550 tig0032425:-424 None:intergenic
TAAAATTATGGGAGTGTTTG+GGG 0.534154 tig0032425:+1583 Msa1449030:three_prime_UTR
TAATTTGATTGAACAAATGG+TGG 0.535554 tig0032425:+1453 Msa1449030:CDS
AAGAGTCCTTCTGATGTAGC+AGG 0.542729 tig0032425:-1323 None:intergenic
TGCATCAAACTCAGCACTTG+AGG 0.543786 tig0032425:+1378 Msa1449030:CDS
CTAAAGTGGAACATTAGCAT+AGG 0.544925 tig0032425:+1346 Msa1449030:CDS
GTATGCATGGAGATCTTCGA+AGG 0.556865 tig0032425:+264 Msa1449030:CDS
ATCCTTCTTTCCATCCGTGA+AGG 0.566942 tig0032425:+414 Msa1449030:CDS
TGTAGGCCTGCTACATCAGA+AGG 0.570024 tig0032425:+1317 Msa1449030:intron
AATTTGATTGAACAAATGGT+GGG 0.585223 tig0032425:+1454 Msa1449030:CDS
GGATTGGTCTGTAATTGTTG+AGG 0.607602 tig0032425:+1399 Msa1449030:CDS
TTTCTCGCTCACATCTGCCG+GGG 0.608757 tig0032425:-26 None:intergenic
ATGGTCTTCATTGAACACTC+AGG 0.616881 tig0032425:-210 None:intergenic
CACGGATGGAAAGAAGGATA+CGG 0.617443 tig0032425:-410 None:intergenic
TAATTGTTGAGGATGGAAAG+AGG 0.621845 tig0032425:+1410 Msa1449030:CDS
AATTGGGTGCATAAATCCCA+AGG 0.639199 tig0032425:+179 Msa1449030:CDS
CATACAAAGCATAACTGATG+AGG 0.653511 tig0032425:+230 Msa1449030:CDS
AATGGTGGGAATGGGTTGGG+TGG 0.655520 tig0032425:+1468 Msa1449030:CDS
AAAGTGGAACATTAGCATAG+GGG 0.696426 tig0032425:+1348 Msa1449030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTATGATTAATTAAAGA+TGG + tig0032425:1537-1556 Msa1449030:exon 15.0%
!! TAAAAATACATTACAAATCA+TGG - tig0032425:1683-1702 None:intergenic 15.0%
!! TGTTTATGATTAATTAAAGA+TGG + tig0032425:1537-1556 Msa1449030:exon 15.0%
!! TAAAAATACATTACAAATCA+TGG - tig0032425:1683-1702 None:intergenic 15.0%
!! AAAGAAGTAATCAACTTTAT+GGG + tig0032425:46-65 Msa1449030:exon 20.0%
!! AGAATACAAAACTACTTTAT+AGG - tig0032425:343-362 None:intergenic 20.0%
!!! AAGTAGTTTTGTATTCTAAT+TGG + tig0032425:346-365 Msa1449030:intron 20.0%
!! TGAATCAAAGCATATAATTA+TGG + tig0032425:440-459 Msa1449030:CDS 20.0%
!! GAATCAAAGCATATAATTAT+GGG + tig0032425:441-460 Msa1449030:CDS 20.0%
!! TGTTAGACATATTTCATTAT+AGG - tig0032425:1141-1160 None:intergenic 20.0%
!! AGATAATTCACTAATTAAGA+AGG - tig0032425:1185-1204 None:intergenic 20.0%
!! ATCATTAGAATAAGTAAATC+AGG - tig0032425:1257-1276 None:intergenic 20.0%
!!! GAAATGTTTTTTCAATTTGT+AGG + tig0032425:1300-1319 Msa1449030:intron 20.0%
!! AACTAATTTGATTGAACAAA+TGG + tig0032425:1450-1469 Msa1449030:CDS 20.0%
!!! CTCATTTTTTTTATACAGTT+AGG - tig0032425:1636-1655 None:intergenic 20.0%
!!! AAAAGTAAAGTTTATTGGTT+TGG - tig0032425:1711-1730 None:intergenic 20.0%
!! AAAGAAGTAATCAACTTTAT+GGG + tig0032425:46-65 Msa1449030:exon 20.0%
!! AGAATACAAAACTACTTTAT+AGG - tig0032425:343-362 None:intergenic 20.0%
!!! AAGTAGTTTTGTATTCTAAT+TGG + tig0032425:346-365 Msa1449030:intron 20.0%
!! TGAATCAAAGCATATAATTA+TGG + tig0032425:440-459 Msa1449030:CDS 20.0%
!! GAATCAAAGCATATAATTAT+GGG + tig0032425:441-460 Msa1449030:CDS 20.0%
!! TGTTAGACATATTTCATTAT+AGG - tig0032425:1141-1160 None:intergenic 20.0%
!! AGATAATTCACTAATTAAGA+AGG - tig0032425:1185-1204 None:intergenic 20.0%
!! ATCATTAGAATAAGTAAATC+AGG - tig0032425:1257-1276 None:intergenic 20.0%
!!! GAAATGTTTTTTCAATTTGT+AGG + tig0032425:1300-1319 Msa1449030:intron 20.0%
!! AACTAATTTGATTGAACAAA+TGG + tig0032425:1450-1469 Msa1449030:CDS 20.0%
!!! CTCATTTTTTTTATACAGTT+AGG - tig0032425:1636-1655 None:intergenic 20.0%
!!! AAAAGTAAAGTTTATTGGTT+TGG - tig0032425:1711-1730 None:intergenic 20.0%
! GAAAGAAGTAATCAACTTTA+TGG + tig0032425:45-64 Msa1449030:exon 25.0%
! TTGATTCAGATAAAAGCTTA+TGG + tig0032425:148-167 Msa1449030:intron 25.0%
!! TTAAGTTGTTTAACCAAAGA+GGG - tig0032425:616-635 None:intergenic 25.0%
!! ATTAAGTTGTTTAACCAAAG+AGG - tig0032425:617-636 None:intergenic 25.0%
!! AGTCAAACTGTTTTCATATA+AGG + tig0032425:697-716 Msa1449030:intron 25.0%
!!! AGCATATAGCTTCTTTTTTT+AGG - tig0032425:851-870 None:intergenic 25.0%
! GTTTATTTGAACTTATCTAC+TGG - tig0032425:984-1003 None:intergenic 25.0%
! TCAAATAAACCAATTCAACT+AGG + tig0032425:994-1013 Msa1449030:intron 25.0%
! ACATACAAAGTCAAGTTTAA+TGG - tig0032425:1039-1058 None:intergenic 25.0%
! TTAGTGAATTATCTTCCATT+TGG + tig0032425:1191-1210 Msa1449030:intron 25.0%
! TCCATTTGGTCTATATAATA+TGG + tig0032425:1205-1224 Msa1449030:intron 25.0%
! CCATTTGGTCTATATAATAT+GGG + tig0032425:1206-1225 Msa1449030:intron 25.0%
! TAATTTGATTGAACAAATGG+TGG + tig0032425:1453-1472 Msa1449030:CDS 25.0%
! AATTTGATTGAACAAATGGT+GGG + tig0032425:1454-1473 Msa1449030:CDS 25.0%
!! GATGGTTTTCAAAATTTTGT+TGG + tig0032425:1555-1574 Msa1449030:three_prime_UTR 25.0%
!!! TTAAAATTATGGGAGTGTTT+GGG + tig0032425:1582-1601 Msa1449030:three_prime_UTR 25.0%
! GAGAGAAAAGTAAAGTTTAT+TGG - tig0032425:1716-1735 None:intergenic 25.0%
! GAAAGAAGTAATCAACTTTA+TGG + tig0032425:45-64 Msa1449030:exon 25.0%
! TTGATTCAGATAAAAGCTTA+TGG + tig0032425:148-167 Msa1449030:intron 25.0%
!! TTAAGTTGTTTAACCAAAGA+GGG - tig0032425:616-635 None:intergenic 25.0%
!! ATTAAGTTGTTTAACCAAAG+AGG - tig0032425:617-636 None:intergenic 25.0%
!! AGTCAAACTGTTTTCATATA+AGG + tig0032425:697-716 Msa1449030:intron 25.0%
!!! AGCATATAGCTTCTTTTTTT+AGG - tig0032425:851-870 None:intergenic 25.0%
! GTTTATTTGAACTTATCTAC+TGG - tig0032425:984-1003 None:intergenic 25.0%
! TCAAATAAACCAATTCAACT+AGG + tig0032425:994-1013 Msa1449030:intron 25.0%
! ACATACAAAGTCAAGTTTAA+TGG - tig0032425:1039-1058 None:intergenic 25.0%
! TTAGTGAATTATCTTCCATT+TGG + tig0032425:1191-1210 Msa1449030:intron 25.0%
! TCCATTTGGTCTATATAATA+TGG + tig0032425:1205-1224 Msa1449030:intron 25.0%
! CCATTTGGTCTATATAATAT+GGG + tig0032425:1206-1225 Msa1449030:intron 25.0%
! TAATTTGATTGAACAAATGG+TGG + tig0032425:1453-1472 Msa1449030:CDS 25.0%
! AATTTGATTGAACAAATGGT+GGG + tig0032425:1454-1473 Msa1449030:CDS 25.0%
!! GATGGTTTTCAAAATTTTGT+TGG + tig0032425:1555-1574 Msa1449030:three_prime_UTR 25.0%
!!! TTAAAATTATGGGAGTGTTT+GGG + tig0032425:1582-1601 Msa1449030:three_prime_UTR 25.0%
! GAGAGAAAAGTAAAGTTTAT+TGG - tig0032425:1716-1735 None:intergenic 25.0%
ATCAACTTTATGGGAGTTAA+AGG + tig0032425:55-74 Msa1449030:exon 30.0%
TTTATGCACCCAATTCTAAT+TGG - tig0032425:174-193 None:intergenic 30.0%
GGATTATTACTTTGTATGCA+TGG + tig0032425:251-270 Msa1449030:CDS 30.0%
TTCGAAGGATTAAAAAGAAC+TGG + tig0032425:279-298 Msa1449030:CDS 30.0%
GCATGATTATGAGATTATCT+TGG + tig0032425:310-329 Msa1449030:intron 30.0%
! ATTTTGCTTCTTTTTGTCTG+TGG + tig0032425:374-393 Msa1449030:intron 30.0%
!! TATAATTATGGGTTTGTTGC+AGG + tig0032425:452-471 Msa1449030:CDS 30.0%
CCCATATTATATAGACCAAA+TGG - tig0032425:1209-1228 None:intergenic 30.0%
GAATAAGTAAATCAGGTTTC+AGG - tig0032425:1250-1269 None:intergenic 30.0%
TAAAGTGGAACATTAGCATA+GGG + tig0032425:1347-1366 Msa1449030:CDS 30.0%
TTTATTGTCAAAGCAAGAAC+AGG + tig0032425:1501-1520 Msa1449030:CDS 30.0%
!! TTGTTGGCTCCTTAAAATTA+TGG + tig0032425:1571-1590 Msa1449030:three_prime_UTR 30.0%
!! TGTTGGCTCCTTAAAATTAT+GGG + tig0032425:1572-1591 Msa1449030:three_prime_UTR 30.0%
! CAAACACTCCCATAATTTTA+AGG - tig0032425:1583-1602 None:intergenic 30.0%
!! CTTAAAATTATGGGAGTGTT+TGG + tig0032425:1581-1600 Msa1449030:three_prime_UTR 30.0%
!! TAAAATTATGGGAGTGTTTG+GGG + tig0032425:1583-1602 Msa1449030:three_prime_UTR 30.0%
! TTTGACTTTAACACTCTAGA+TGG - tig0032425:1752-1771 None:intergenic 30.0%
ATCAACTTTATGGGAGTTAA+AGG + tig0032425:55-74 Msa1449030:exon 30.0%
TTTATGCACCCAATTCTAAT+TGG - tig0032425:174-193 None:intergenic 30.0%
GGATTATTACTTTGTATGCA+TGG + tig0032425:251-270 Msa1449030:CDS 30.0%
TTCGAAGGATTAAAAAGAAC+TGG + tig0032425:279-298 Msa1449030:CDS 30.0%
GCATGATTATGAGATTATCT+TGG + tig0032425:310-329 Msa1449030:intron 30.0%
! ATTTTGCTTCTTTTTGTCTG+TGG + tig0032425:374-393 Msa1449030:intron 30.0%
!! TATAATTATGGGTTTGTTGC+AGG + tig0032425:452-471 Msa1449030:CDS 30.0%
CCCATATTATATAGACCAAA+TGG - tig0032425:1209-1228 None:intergenic 30.0%
GAATAAGTAAATCAGGTTTC+AGG - tig0032425:1250-1269 None:intergenic 30.0%
TAAAGTGGAACATTAGCATA+GGG + tig0032425:1347-1366 Msa1449030:CDS 30.0%
TTTATTGTCAAAGCAAGAAC+AGG + tig0032425:1501-1520 Msa1449030:CDS 30.0%
!! TTGTTGGCTCCTTAAAATTA+TGG + tig0032425:1571-1590 Msa1449030:three_prime_UTR 30.0%
!! TGTTGGCTCCTTAAAATTAT+GGG + tig0032425:1572-1591 Msa1449030:three_prime_UTR 30.0%
! CAAACACTCCCATAATTTTA+AGG - tig0032425:1583-1602 None:intergenic 30.0%
!! CTTAAAATTATGGGAGTGTT+TGG + tig0032425:1581-1600 Msa1449030:three_prime_UTR 30.0%
!! TAAAATTATGGGAGTGTTTG+GGG + tig0032425:1583-1602 Msa1449030:three_prime_UTR 30.0%
! TTTGACTTTAACACTCTAGA+TGG - tig0032425:1752-1771 None:intergenic 30.0%
GAGTTAAAGGTAAAATGCGA+CGG + tig0032425:68-87 Msa1449030:intron 35.0%
TTCGTGTTTGATACTTTCCA+TGG + tig0032425:100-119 Msa1449030:intron 35.0%
AGCTTATGGCCAATTAGAAT+TGG + tig0032425:162-181 Msa1449030:CDS 35.0%
GCTTATGGCCAATTAGAATT+GGG + tig0032425:163-182 Msa1449030:CDS 35.0%
TCATTGAACACTCAGGTATT+CGG - tig0032425:206-225 None:intergenic 35.0%
CTCATCAGTTATGCTTTGTA+TGG - tig0032425:232-251 None:intergenic 35.0%
CATACAAAGCATAACTGATG+AGG + tig0032425:230-249 Msa1449030:CDS 35.0%
ATTGAAAGAAGGAACATGAG+AGG - tig0032425:563-582 None:intergenic 35.0%
CGTAACATGACATTGAAAGA+AGG - tig0032425:574-593 None:intergenic 35.0%
CTTTCAATGTCATGTTACGT+CGG + tig0032425:575-594 Msa1449030:intron 35.0%
AGAACATGAGTTCGAATCAT+GGG + tig0032425:738-757 Msa1449030:intron 35.0%
AGGTAAATAAAGTCTGGCAA+AGG - tig0032425:773-792 None:intergenic 35.0%
!!! TTTTTTTAGGTCTTACCCTC+CGG - tig0032425:838-857 None:intergenic 35.0%
! TTTCCATAAGCTTTCTAGGA+TGG - tig0032425:885-904 None:intergenic 35.0%
CTCATTTCCATAAGCTTTCT+AGG - tig0032425:889-908 None:intergenic 35.0%
!! TGTTTTGGACCTAGTTGAAT+TGG - tig0032425:1006-1025 None:intergenic 35.0%
ACTAGGTCCAAAACAGTTAT+AGG + tig0032425:1011-1030 Msa1449030:intron 35.0%
CTAGGTCCAAAACAGTTATA+GGG + tig0032425:1012-1031 Msa1449030:intron 35.0%
! AATGGACCCTATAACTGTTT+TGG - tig0032425:1021-1040 None:intergenic 35.0%
TCTTATCACATGAGAGGAAA+GGG - tig0032425:1075-1094 None:intergenic 35.0%
TTCTTATCACATGAGAGGAA+AGG - tig0032425:1076-1095 None:intergenic 35.0%
TTCCTCTCATGTGATAAGAA+AGG + tig0032425:1076-1095 Msa1449030:intron 35.0%
CTAAAGTGGAACATTAGCAT+AGG + tig0032425:1346-1365 Msa1449030:CDS 35.0%
AAAGTGGAACATTAGCATAG+GGG + tig0032425:1348-1367 Msa1449030:CDS 35.0%
TAATTGTTGAGGATGGAAAG+AGG + tig0032425:1410-1429 Msa1449030:CDS 35.0%
! ATTGAACAAATGGTGGGAAT+GGG + tig0032425:1460-1479 Msa1449030:CDS 35.0%
GAGTTAAAGGTAAAATGCGA+CGG + tig0032425:68-87 Msa1449030:intron 35.0%
TTCGTGTTTGATACTTTCCA+TGG + tig0032425:100-119 Msa1449030:intron 35.0%
AGCTTATGGCCAATTAGAAT+TGG + tig0032425:162-181 Msa1449030:CDS 35.0%
GCTTATGGCCAATTAGAATT+GGG + tig0032425:163-182 Msa1449030:CDS 35.0%
TCATTGAACACTCAGGTATT+CGG - tig0032425:206-225 None:intergenic 35.0%
CTCATCAGTTATGCTTTGTA+TGG - tig0032425:232-251 None:intergenic 35.0%
CATACAAAGCATAACTGATG+AGG + tig0032425:230-249 Msa1449030:CDS 35.0%
ATTGAAAGAAGGAACATGAG+AGG - tig0032425:563-582 None:intergenic 35.0%
CGTAACATGACATTGAAAGA+AGG - tig0032425:574-593 None:intergenic 35.0%
CTTTCAATGTCATGTTACGT+CGG + tig0032425:575-594 Msa1449030:intron 35.0%
AGAACATGAGTTCGAATCAT+GGG + tig0032425:738-757 Msa1449030:intron 35.0%
AGGTAAATAAAGTCTGGCAA+AGG - tig0032425:773-792 None:intergenic 35.0%
!!! TTTTTTTAGGTCTTACCCTC+CGG - tig0032425:838-857 None:intergenic 35.0%
! TTTCCATAAGCTTTCTAGGA+TGG - tig0032425:885-904 None:intergenic 35.0%
CTCATTTCCATAAGCTTTCT+AGG - tig0032425:889-908 None:intergenic 35.0%
!! TGTTTTGGACCTAGTTGAAT+TGG - tig0032425:1006-1025 None:intergenic 35.0%
ACTAGGTCCAAAACAGTTAT+AGG + tig0032425:1011-1030 Msa1449030:intron 35.0%
CTAGGTCCAAAACAGTTATA+GGG + tig0032425:1012-1031 Msa1449030:intron 35.0%
! AATGGACCCTATAACTGTTT+TGG - tig0032425:1021-1040 None:intergenic 35.0%
TCTTATCACATGAGAGGAAA+GGG - tig0032425:1075-1094 None:intergenic 35.0%
TTCTTATCACATGAGAGGAA+AGG - tig0032425:1076-1095 None:intergenic 35.0%
TTCCTCTCATGTGATAAGAA+AGG + tig0032425:1076-1095 Msa1449030:intron 35.0%
CTAAAGTGGAACATTAGCAT+AGG + tig0032425:1346-1365 Msa1449030:CDS 35.0%
AAAGTGGAACATTAGCATAG+GGG + tig0032425:1348-1367 Msa1449030:CDS 35.0%
TAATTGTTGAGGATGGAAAG+AGG + tig0032425:1410-1429 Msa1449030:CDS 35.0%
! ATTGAACAAATGGTGGGAAT+GGG + tig0032425:1460-1479 Msa1449030:CDS 35.0%
GTTTGATACTTTCCATGGTG+AGG + tig0032425:105-124 Msa1449030:intron 40.0%
AGTTGACTATAACCTCACCA+TGG - tig0032425:120-139 None:intergenic 40.0%
AATTGGGTGCATAAATCCCA+AGG + tig0032425:179-198 Msa1449030:CDS 40.0%
ATGGTCTTCATTGAACACTC+AGG - tig0032425:213-232 None:intergenic 40.0%
AGGATTAAAAAGAACTGGCC+AGG + tig0032425:284-303 Msa1449030:CDS 40.0%
ATCTCATAATCATGCTCACC+TGG - tig0032425:305-324 None:intergenic 40.0%
AAAGACACAAAGTCCCTCTT+TGG + tig0032425:600-619 Msa1449030:intron 40.0%
GAGAACATGAGTTCGAATCA+TGG + tig0032425:737-756 Msa1449030:intron 40.0%
AAGCCATCCTAGAAAGCTTA+TGG + tig0032425:879-898 Msa1449030:intron 40.0%
TTATCACATGAGAGGAAAGG+GGG - tig0032425:1073-1092 None:intergenic 40.0%
CTTATCACATGAGAGGAAAG+GGG - tig0032425:1074-1093 None:intergenic 40.0%
CTCCTTTCTTATCACATGAG+AGG - tig0032425:1081-1100 None:intergenic 40.0%
AAATCAGGTTTCAGGCCAAA+GGG - tig0032425:1242-1261 None:intergenic 40.0%
TAAATCAGGTTTCAGGCCAA+AGG - tig0032425:1243-1262 None:intergenic 40.0%
! TCAGAAGGACTCTTCTAAAG+TGG + tig0032425:1332-1351 Msa1449030:CDS 40.0%
!! GGATTGGTCTGTAATTGTTG+AGG + tig0032425:1399-1418 Msa1449030:CDS 40.0%
TGGTCTGTAATTGTTGAGGA+TGG + tig0032425:1403-1422 Msa1449030:CDS 40.0%
! ATGGAAAGAGGTATGCAGAA+AGG + tig0032425:1422-1441 Msa1449030:CDS 40.0%
! TGGAAAGAGGTATGCAGAAA+GGG + tig0032425:1423-1442 Msa1449030:CDS 40.0%
! GATTGAACAAATGGTGGGAA+TGG + tig0032425:1459-1478 Msa1449030:CDS 40.0%
GGGGTTAACTTTAGCTGAAA+AGG + tig0032425:1602-1621 Msa1449030:three_prime_UTR 40.0%
GTTTGATACTTTCCATGGTG+AGG + tig0032425:105-124 Msa1449030:intron 40.0%
AGTTGACTATAACCTCACCA+TGG - tig0032425:120-139 None:intergenic 40.0%
AATTGGGTGCATAAATCCCA+AGG + tig0032425:179-198 Msa1449030:CDS 40.0%
ATGGTCTTCATTGAACACTC+AGG - tig0032425:213-232 None:intergenic 40.0%
AGGATTAAAAAGAACTGGCC+AGG + tig0032425:284-303 Msa1449030:CDS 40.0%
ATCTCATAATCATGCTCACC+TGG - tig0032425:305-324 None:intergenic 40.0%
AAAGACACAAAGTCCCTCTT+TGG + tig0032425:600-619 Msa1449030:intron 40.0%
GAGAACATGAGTTCGAATCA+TGG + tig0032425:737-756 Msa1449030:intron 40.0%
AAGCCATCCTAGAAAGCTTA+TGG + tig0032425:879-898 Msa1449030:intron 40.0%
TTATCACATGAGAGGAAAGG+GGG - tig0032425:1073-1092 None:intergenic 40.0%
CTTATCACATGAGAGGAAAG+GGG - tig0032425:1074-1093 None:intergenic 40.0%
CTCCTTTCTTATCACATGAG+AGG - tig0032425:1081-1100 None:intergenic 40.0%
AAATCAGGTTTCAGGCCAAA+GGG - tig0032425:1242-1261 None:intergenic 40.0%
TAAATCAGGTTTCAGGCCAA+AGG - tig0032425:1243-1262 None:intergenic 40.0%
! TCAGAAGGACTCTTCTAAAG+TGG + tig0032425:1332-1351 Msa1449030:CDS 40.0%
!! GGATTGGTCTGTAATTGTTG+AGG + tig0032425:1399-1418 Msa1449030:CDS 40.0%
TGGTCTGTAATTGTTGAGGA+TGG + tig0032425:1403-1422 Msa1449030:CDS 40.0%
! ATGGAAAGAGGTATGCAGAA+AGG + tig0032425:1422-1441 Msa1449030:CDS 40.0%
! TGGAAAGAGGTATGCAGAAA+GGG + tig0032425:1423-1442 Msa1449030:CDS 40.0%
! GATTGAACAAATGGTGGGAA+TGG + tig0032425:1459-1478 Msa1449030:CDS 40.0%
GGGGTTAACTTTAGCTGAAA+AGG + tig0032425:1602-1621 Msa1449030:three_prime_UTR 40.0%
GTATGCATGGAGATCTTCGA+AGG + tig0032425:264-283 Msa1449030:CDS 45.0%
CACGGATGGAAAGAAGGATA+CGG - tig0032425:413-432 None:intergenic 45.0%
ATCCTTCTTTCCATCCGTGA+AGG + tig0032425:414-433 Msa1449030:CDS 45.0%
GCTTTGATTCAGCTCCTTCA+CGG - tig0032425:431-450 None:intergenic 45.0%
ACATGAGTTCGAATCATGGG+TGG + tig0032425:741-760 Msa1449030:intron 45.0%
GCTGGGAGGTAAATAAAGTC+TGG - tig0032425:779-798 None:intergenic 45.0%
! GACTTTATTTACCTCCCAGC+CGG + tig0032425:779-798 Msa1449030:intron 45.0%
! TAGCCCTTTTCCTTGAGAAC+CGG + tig0032425:816-835 Msa1449030:intron 45.0%
TATCACATGAGAGGAAAGGG+GGG - tig0032425:1072-1091 None:intergenic 45.0%
AAGAGTCCTTCTGATGTAGC+AGG - tig0032425:1326-1345 None:intergenic 45.0%
TGCATCAAACTCAGCACTTG+AGG + tig0032425:1378-1397 Msa1449030:CDS 45.0%
CAAACTCAGCACTTGAGGAT+TGG + tig0032425:1383-1402 Msa1449030:CDS 45.0%
! AACAAATGGTGGGAATGGGT+TGG + tig0032425:1464-1483 Msa1449030:CDS 45.0%
! ACAAATGGTGGGAATGGGTT+GGG + tig0032425:1465-1484 Msa1449030:CDS 45.0%
GTATGCATGGAGATCTTCGA+AGG + tig0032425:264-283 Msa1449030:CDS 45.0%
CACGGATGGAAAGAAGGATA+CGG - tig0032425:413-432 None:intergenic 45.0%
ATCCTTCTTTCCATCCGTGA+AGG + tig0032425:414-433 Msa1449030:CDS 45.0%
GCTTTGATTCAGCTCCTTCA+CGG - tig0032425:431-450 None:intergenic 45.0%
ACATGAGTTCGAATCATGGG+TGG + tig0032425:741-760 Msa1449030:intron 45.0%
GCTGGGAGGTAAATAAAGTC+TGG - tig0032425:779-798 None:intergenic 45.0%
! GACTTTATTTACCTCCCAGC+CGG + tig0032425:779-798 Msa1449030:intron 45.0%
! TAGCCCTTTTCCTTGAGAAC+CGG + tig0032425:816-835 Msa1449030:intron 45.0%
TATCACATGAGAGGAAAGGG+GGG - tig0032425:1072-1091 None:intergenic 45.0%
AAGAGTCCTTCTGATGTAGC+AGG - tig0032425:1326-1345 None:intergenic 45.0%
TGCATCAAACTCAGCACTTG+AGG + tig0032425:1378-1397 Msa1449030:CDS 45.0%
CAAACTCAGCACTTGAGGAT+TGG + tig0032425:1383-1402 Msa1449030:CDS 45.0%
! AACAAATGGTGGGAATGGGT+TGG + tig0032425:1464-1483 Msa1449030:CDS 45.0%
! ACAAATGGTGGGAATGGGTT+GGG + tig0032425:1465-1484 Msa1449030:CDS 45.0%
GTTTGGATATGTGCCTTCCC+CGG + tig0032425:9-28 Msa1449030:five_prime_UTR 50.0%
TCTTTCTCGCTCACATCTGC+CGG - tig0032425:31-50 None:intergenic 50.0%
CACTCAGGTATTCGGTCCTT+GGG - tig0032425:198-217 None:intergenic 50.0%
ACACTCAGGTATTCGGTCCT+TGG - tig0032425:199-218 None:intergenic 50.0%
CTCCTTCACGGATGGAAAGA+AGG - tig0032425:419-438 None:intergenic 50.0%
TGATTCAGCTCCTTCACGGA+TGG - tig0032425:427-446 None:intergenic 50.0%
GGCTATGATAGTCCGAAGTC+CGG - tig0032425:801-820 None:intergenic 50.0%
CCTCCGGTTCTCAAGGAAAA+GGG - tig0032425:822-841 None:intergenic 50.0%
! CCTTTTCCTTGAGAACCGGA+GGG + tig0032425:820-839 Msa1449030:intron 50.0%
ATGGGAGCGAAACAACCCTT+TGG + tig0032425:1224-1243 Msa1449030:intron 50.0%
TGTAGGCCTGCTACATCAGA+AGG + tig0032425:1317-1336 Msa1449030:intron 50.0%
GTTTGGATATGTGCCTTCCC+CGG + tig0032425:9-28 Msa1449030:five_prime_UTR 50.0%
TCTTTCTCGCTCACATCTGC+CGG - tig0032425:31-50 None:intergenic 50.0%
CACTCAGGTATTCGGTCCTT+GGG - tig0032425:198-217 None:intergenic 50.0%
ACACTCAGGTATTCGGTCCT+TGG - tig0032425:199-218 None:intergenic 50.0%
CTCCTTCACGGATGGAAAGA+AGG - tig0032425:419-438 None:intergenic 50.0%
TGATTCAGCTCCTTCACGGA+TGG - tig0032425:427-446 None:intergenic 50.0%
GGCTATGATAGTCCGAAGTC+CGG - tig0032425:801-820 None:intergenic 50.0%
CCTCCGGTTCTCAAGGAAAA+GGG - tig0032425:822-841 None:intergenic 50.0%
! CCTTTTCCTTGAGAACCGGA+GGG + tig0032425:820-839 Msa1449030:intron 50.0%
ATGGGAGCGAAACAACCCTT+TGG + tig0032425:1224-1243 Msa1449030:intron 50.0%
TGTAGGCCTGCTACATCAGA+AGG + tig0032425:1317-1336 Msa1449030:intron 50.0%
TTTCTCGCTCACATCTGCCG+GGG - tig0032425:29-48 None:intergenic 55.0%
CTTTCTCGCTCACATCTGCC+GGG - tig0032425:30-49 None:intergenic 55.0%
TTTACCTCCCAGCCGGACTT+CGG + tig0032425:786-805 Msa1449030:intron 55.0%
TGATAGTCCGAAGTCCGGCT+GGG - tig0032425:796-815 None:intergenic 55.0%
ATGATAGTCCGAAGTCCGGC+TGG - tig0032425:797-816 None:intergenic 55.0%
! CCCTTTTCCTTGAGAACCGG+AGG + tig0032425:819-838 Msa1449030:intron 55.0%
CCCTCCGGTTCTCAAGGAAA+AGG - tig0032425:823-842 None:intergenic 55.0%
GTCTTACCCTCCGGTTCTCA+AGG - tig0032425:829-848 None:intergenic 55.0%
!! AATGGTGGGAATGGGTTGGG+TGG + tig0032425:1468-1487 Msa1449030:CDS 55.0%
TTTCTCGCTCACATCTGCCG+GGG - tig0032425:29-48 None:intergenic 55.0%
CTTTCTCGCTCACATCTGCC+GGG - tig0032425:30-49 None:intergenic 55.0%
TTTACCTCCCAGCCGGACTT+CGG + tig0032425:786-805 Msa1449030:intron 55.0%
TGATAGTCCGAAGTCCGGCT+GGG - tig0032425:796-815 None:intergenic 55.0%
ATGATAGTCCGAAGTCCGGC+TGG - tig0032425:797-816 None:intergenic 55.0%
! CCCTTTTCCTTGAGAACCGG+AGG + tig0032425:819-838 Msa1449030:intron 55.0%
CCCTCCGGTTCTCAAGGAAA+AGG - tig0032425:823-842 None:intergenic 55.0%
GTCTTACCCTCCGGTTCTCA+AGG - tig0032425:829-848 None:intergenic 55.0%
!! AATGGTGGGAATGGGTTGGG+TGG + tig0032425:1468-1487 Msa1449030:CDS 55.0%
TCGCTCACATCTGCCGGGGA+AGG - tig0032425:25-44 None:intergenic 65.0%
TAGTCCGAAGTCCGGCTGGG+AGG - tig0032425:793-812 None:intergenic 65.0%
TCGCTCACATCTGCCGGGGA+AGG - tig0032425:25-44 None:intergenic 65.0%
TAGTCCGAAGTCCGGCTGGG+AGG - tig0032425:793-812 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0032425 gene 7 1814 7 ID=Msa1449030;Name=Msa1449030
tig0032425 mRNA 7 1814 7 ID=Msa1449030-mRNA-1;Parent=Msa1449030;Name=Msa1449030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=57|1|1|1|1|0.75|4|265|151
tig0032425 exon 7 76 7 ID=Msa1449030-mRNA-1:exon:16746;Parent=Msa1449030-mRNA-1
tig0032425 exon 157 305 157 ID=Msa1449030-mRNA-1:exon:16747;Parent=Msa1449030-mRNA-1
tig0032425 exon 408 473 408 ID=Msa1449030-mRNA-1:exon:16748;Parent=Msa1449030-mRNA-1
tig0032425 exon 1322 1814 1322 ID=Msa1449030-mRNA-1:exon:16749;Parent=Msa1449030-mRNA-1
tig0032425 five_prime_UTR 7 63 7 ID=Msa1449030-mRNA-1:five_prime_utr;Parent=Msa1449030-mRNA-1
tig0032425 CDS 64 76 64 ID=Msa1449030-mRNA-1:cds;Parent=Msa1449030-mRNA-1
tig0032425 CDS 157 305 157 ID=Msa1449030-mRNA-1:cds;Parent=Msa1449030-mRNA-1
tig0032425 CDS 408 473 408 ID=Msa1449030-mRNA-1:cds;Parent=Msa1449030-mRNA-1
tig0032425 CDS 1322 1549 1322 ID=Msa1449030-mRNA-1:cds;Parent=Msa1449030-mRNA-1
tig0032425 three_prime_UTR 1550 1814 1550 ID=Msa1449030-mRNA-1:three_prime_utr;Parent=Msa1449030-mRNA-1
Gene Sequence

>Msa1449030

ATGGGAGTTAAAGATAAAAGCTTATGGCCAATTAGAATTGGGTGCATAAATCCCAAGGACCGAATACCTGAGTGTTCAATGAAGACCATACAAAGCATAACTGATGAGGATTATTACTTTGTATGCATGGAGATCTTCGAAGGATTAAAAAGAACTGGCCAGTTCCGTATCCTTCTTTCCATCCGTGAAGGAGCTGAATCAAAGCATATAATTATGGGTTTGTTGCAGGCCTGCTACATCAGAAGGACTCTTCTAAAGTGGAACATTAGCATAGGGGAAAACAATGCATCAAACTCAGCACTTGAGGATTGGTCTGTAATTGTTGAGGATGGAAAGAGGTATGCAGAAAGGGATGTAACTAATTTGATTGAACAAATGGTGGGAATGGGTTGGGTGGTGAAGAACATTTTATTGTCAAAGCAAGAACAGGCAAGATACAGTTTTGTTTATGATTAA
Protein sequence

>Msa1449030

MGVKDKSLWPIRIGCINPKDRIPECSMKTIQSITDEDYYFVCMEIFEGLKRTGQFRILLSIREGAESKHIIMGLLQACYIRRTLLKWNISIGENNASNSALEDWSVIVEDGKRYAERDVTNLIEQMVGMGWVVKNILLSKQEQARYSFVYD*