AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0723140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0723140 MtrunA17_Chr5g0413961 98.413 126 2 0 1 126 1 126 2.48e-80 240
Msa0723140 MtrunA17_Chr3g0126101 49.558 113 57 0 14 126 6 118 9.98e-32 114
Msa0723140 MtrunA17_Chr6g0451261 47.826 115 59 1 13 126 10 124 8.19e-26 101
Msa0723140 MtrunA17_Chr4g0059311 47.863 117 59 2 12 126 9 125 2.84e-25 97.8
Msa0723140 MtrunA17_Chr6g0451171 44.828 116 63 1 12 126 10 125 3.42e-23 92.4
Msa0723140 MtrunA17_Chr6g0451181 43.966 116 64 1 12 126 10 125 6.03e-23 87.0
Msa0723140 MtrunA17_Chr5g0413921 49.020 102 51 1 26 126 19 120 6.38e-23 91.7
Msa0723140 MtrunA17_Chr6g0451201 50.515 97 47 1 30 126 28 123 9.33e-23 91.3
Msa0723140 MtrunA17_Chr6g0451211 51.546 97 42 2 34 126 32 127 2.55e-22 90.1
Msa0723140 MtrunA17_Chr7g0227921 46.610 118 60 2 11 126 8 124 9.92e-22 88.6
Msa0723140 MtrunA17_Chr6g0451251 48.421 95 47 1 34 126 32 126 3.49e-21 87.0
Msa0723140 MtrunA17_Chr3g0081421 35.398 113 72 1 15 126 18 130 3.92e-20 84.3
Msa0723140 MtrunA17_Chr5g0413941 39.823 113 67 1 18 129 11 123 4.08e-20 84.0
Msa0723140 MtrunA17_Chr5g0413931 41.964 112 64 1 19 129 11 122 1.53e-19 82.4
Msa0723140 MtrunA17_Chr7g0255561 35.043 117 75 1 11 126 11 127 2.50e-19 82.0
Msa0723140 MtrunA17_Chr7g0255581 35.043 117 75 1 11 126 11 127 2.50e-19 82.0
Msa0723140 MtrunA17_Chr5g0413951 40.179 112 66 1 19 129 12 123 3.10e-19 81.6
Msa0723140 MtrunA17_Chr3g0081511 36.283 113 71 1 15 126 18 130 8.31e-19 80.5
Msa0723140 MtrunA17_Chr1g0150151 41.525 118 67 1 11 126 8 125 1.01e-18 80.1
Msa0723140 MtrunA17_Chr8g0354121 34.167 120 78 1 8 126 7 126 2.86e-18 79.3
Msa0723140 MtrunA17_Chr5g0404801 39.806 103 61 1 25 126 18 120 4.78e-18 74.3
Msa0723140 MtrunA17_Chr2g0278541 38.843 121 73 1 8 127 6 126 5.28e-18 78.6
Msa0723140 MtrunA17_Chr5g0408031 38.462 117 71 1 9 124 5 121 3.65e-17 75.9
Msa0723140 MtrunA17_Chr3g0142131 43.564 101 56 1 27 126 33 133 8.95e-17 75.1
Msa0723140 MtrunA17_Chr1g0155761 39.316 117 70 1 11 126 18 134 1.19e-16 74.7
Msa0723140 MtrunA17_Chr7g0271561 32.773 119 79 1 9 126 2 120 1.77e-16 73.9
Msa0723140 MtrunA17_Chr2g0278031 36.207 116 73 1 7 121 7 122 2.92e-16 69.7
Msa0723140 MtrunA17_Chr1g0177221 37.500 104 64 1 24 126 28 131 9.46e-16 71.2
Msa0723140 MtrunA17_Chr3g0126091 34.711 121 75 2 10 126 7 127 2.11e-15 70.9
Msa0723140 MtrunA17_Chr1g0171301 32.500 120 80 1 11 129 13 132 3.02e-15 70.5
Msa0723140 MtrunA17_Chr5g0408001 36.975 119 74 1 9 126 5 123 3.82e-15 70.5
Msa0723140 MtrunA17_Chr6g0483401 46.535 101 53 1 27 126 23 123 7.35e-15 69.3
Msa0723140 MtrunA17_Chr1g0160181 38.614 101 61 1 27 126 31 131 8.24e-15 69.3
Msa0723140 MtrunA17_Chr2g0284261 32.500 120 80 1 7 125 1 120 1.36e-14 68.6
Msa0723140 MtrunA17_Chr5g0408041 39.216 102 61 1 24 124 21 122 1.44e-14 68.9
Msa0723140 MtrunA17_Chr2g0278561 36.607 112 70 1 13 123 14 125 2.78e-14 67.8
Msa0723140 MtrunA17_Chr1g0182291 34.167 120 78 1 8 126 9 128 3.00e-14 67.8
Msa0723140 MtrunA17_Chr1g0150641 56.604 53 23 0 76 128 2 54 3.58e-14 62.4
Msa0723140 MtrunA17_Chr1g0186611 32.759 116 77 1 15 129 17 132 3.84e-14 67.4
Msa0723140 MtrunA17_Chr1g0160171 31.707 123 77 2 8 126 10 129 4.01e-14 67.4
Msa0723140 MtrunA17_Chr1g0186641 32.231 121 78 2 11 127 7 127 7.73e-14 66.6
Msa0723140 MtrunA17_Chr4g0050891 31.092 119 81 1 8 125 7 125 8.58e-14 66.6
Msa0723140 MtrunA17_Chr4g0050881 39.623 106 54 3 27 127 22 122 9.41e-14 66.2
Msa0723140 MtrunA17_Chr3g0114711 32.500 120 80 1 8 126 8 127 3.43e-13 64.7
Msa0723140 MtrunA17_Chr1g0186571 30.252 119 82 1 12 129 11 129 4.83e-13 64.3
Msa0723140 MtrunA17_Chr2g0278551 36.207 116 73 1 13 127 11 126 5.20e-13 64.3
Msa0723140 MtrunA17_Chr3g0114691 30.172 116 80 1 12 126 18 133 5.40e-13 64.3
Msa0723140 MtrunA17_Chr1g0186601 29.508 122 85 1 9 129 11 132 6.07e-13 63.9
Msa0723140 MtrunA17_Chr3g0114731 31.858 113 76 1 15 126 19 131 6.60e-13 63.9
Msa0723140 MtrunA17_Chr5g0407991 38.776 98 59 1 28 124 23 120 9.29e-13 63.5
Msa0723140 MtrunA17_Chr2g0332001 36.111 108 68 1 19 125 43 150 9.73e-13 63.5
Msa0723140 MtrunA17_Chr7g0263101 29.204 113 79 1 15 126 14 126 4.60e-12 61.6
Msa0723140 MtrunA17_Chr4g0050941 33.058 121 79 2 8 126 6 126 5.26e-12 61.6
Msa0723140 MtrunA17_Chr6g0463971 33.043 115 75 2 11 124 13 126 5.78e-12 61.2
Msa0723140 MtrunA17_Chr1g0184221 27.481 131 80 2 11 126 13 143 8.94e-12 60.8
Msa0723140 MtrunA17_Chr8g0369841 36.449 107 67 1 19 124 14 120 1.10e-11 60.5
Msa0723140 MtrunA17_Chr4g0056081 32.812 128 84 2 1 126 1 128 1.17e-11 60.5
Msa0723140 MtrunA17_Chr5g0407971 34.188 117 76 1 9 124 6 122 1.25e-11 60.5
Msa0723140 MtrunA17_Chr8g0334761 36.522 115 71 2 14 126 10 124 1.77e-11 60.1
Msa0723140 MtrunA17_Chr1g0160111 35.106 94 60 1 34 126 27 120 2.17e-11 59.7
Msa0723140 MtrunA17_Chr1g0211801 31.148 122 81 2 5 125 2 121 2.87e-11 58.9
Msa0723140 MtrunA17_Chr1g0186561 31.933 119 77 2 15 129 14 132 3.22e-11 59.3
Msa0723140 MtrunA17_Chr3g0129571 30.252 119 80 2 11 126 4 122 5.71e-11 58.5
Msa0723140 MtrunA17_Chr6g0451221 58.333 48 19 1 79 126 3 49 5.76e-11 53.9
Msa0723140 MtrunA17_Chr3g0129601 34.043 94 61 1 34 126 49 142 8.60e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0723140 AT5G40210.1 47.706 109 56 1 19 126 17 125 1.76e-24 95.9
Msa0723140 AT5G40240.1 47.458 118 61 1 10 126 15 132 2.51e-22 90.5
Msa0723140 AT5G40240.3 47.458 118 61 1 10 126 15 132 2.51e-22 90.5
Msa0723140 AT5G40240.2 47.458 118 61 1 10 126 29 146 2.74e-22 90.5
Msa0723140 AT3G28050.1 47.009 117 59 2 12 126 9 124 1.87e-21 88.2
Msa0723140 AT5G40230.1 43.966 116 64 1 12 126 18 133 8.08e-21 86.3
Msa0723140 AT1G09380.1 36.752 117 73 1 11 126 4 120 3.32e-20 84.7
Msa0723140 AT5G07050.1 36.283 113 71 1 15 126 18 130 6.61e-20 84.3
Msa0723140 AT1G21890.2 38.462 117 71 1 11 126 7 123 8.64e-19 80.5
Msa0723140 AT1G21890.1 38.462 117 71 1 11 126 7 123 1.20e-18 80.5
Msa0723140 AT3G56620.1 36.283 113 71 1 15 126 11 123 1.81e-18 80.1
Msa0723140 AT4G08290.2 34.188 117 76 1 11 126 9 125 4.86e-17 75.1
Msa0723140 AT4G08290.1 34.188 117 76 1 11 126 9 125 1.44e-16 74.7
Msa0723140 AT3G18200.2 43.564 101 56 1 27 126 47 147 1.52e-16 74.7
Msa0723140 AT5G13670.1 33.333 117 77 1 11 126 4 120 1.97e-16 74.3
Msa0723140 AT2G40900.1 36.283 113 71 1 15 126 11 123 1.98e-16 74.3
Msa0723140 AT2G37460.1 34.454 119 71 2 11 125 11 126 2.93e-16 73.9
Msa0723140 AT4G15540.1 42.241 116 66 1 12 126 14 129 6.93e-16 72.8
Msa0723140 AT4G15540.2 42.241 116 66 1 12 126 14 129 6.93e-16 72.8
Msa0723140 AT1G75500.1 38.462 117 71 1 11 126 16 132 8.17e-16 72.8
Msa0723140 AT1G75500.2 38.462 117 71 1 11 126 16 132 8.17e-16 72.8
Msa0723140 AT2G39510.1 32.743 113 75 1 15 126 8 120 1.72e-15 71.6
Msa0723140 AT3G30340.2 38.144 97 59 1 31 126 27 123 1.76e-15 70.9
Msa0723140 AT4G01450.3 36.752 117 73 1 12 127 7 123 2.03e-15 70.5
Msa0723140 AT3G28100.2 51.000 100 48 1 28 126 27 126 2.05e-15 70.5
Msa0723140 AT3G28100.3 53.191 94 43 1 34 126 33 126 2.05e-15 70.5
Msa0723140 AT4G01440.4 39.450 109 63 2 19 126 16 122 2.23e-15 70.5
Msa0723140 AT4G01450.4 36.752 117 73 1 12 127 7 123 2.44e-15 70.5
Msa0723140 AT1G01070.1 34.188 117 76 1 11 126 12 128 2.78e-15 71.2
Msa0723140 AT3G30340.1 38.144 97 59 1 31 126 27 123 3.03e-15 70.9
Msa0723140 AT4G01440.3 39.450 109 63 2 19 126 16 122 3.11e-15 70.5
Msa0723140 AT3G28100.1 53.191 94 43 1 34 126 33 126 3.87e-15 70.5
Msa0723140 AT4G01440.1 39.450 109 63 2 19 126 16 122 4.26e-15 70.5
Msa0723140 AT4G01450.2 36.752 117 73 1 12 127 7 123 4.59e-15 70.5
Msa0723140 AT4G01450.1 36.752 117 73 1 12 127 7 123 4.61e-15 70.5
Msa0723140 AT1G11450.4 35.043 117 75 1 11 126 12 128 1.46e-14 68.9
Msa0723140 AT1G11450.3 35.043 117 75 1 11 126 12 128 1.65e-14 68.9
Msa0723140 AT4G01430.2 35.398 113 72 1 15 126 9 121 1.97e-14 68.6
Msa0723140 AT4G01430.1 35.398 113 72 1 15 126 9 121 2.35e-14 68.6
Msa0723140 AT1G44800.1 30.769 117 80 1 11 126 7 123 1.10e-13 66.6
Msa0723140 AT1G11460.1 34.188 117 76 1 11 126 12 128 1.67e-13 65.9
Msa0723140 AT4G08300.1 32.231 121 73 2 11 126 7 123 3.26e-13 65.1
Msa0723140 AT4G28040.1 32.231 121 77 3 11 127 4 123 3.77e-12 62.4
Msa0723140 AT4G28040.5 32.231 121 77 3 11 127 4 123 3.77e-12 62.4
Msa0723140 AT4G28040.3 32.231 121 77 3 11 127 4 123 3.77e-12 62.4
Msa0723140 AT4G28040.4 32.231 121 77 3 11 127 4 123 3.77e-12 62.4
Msa0723140 AT4G28040.2 32.231 121 77 3 11 127 4 123 3.77e-12 62.4
Msa0723140 AT5G45370.1 39.000 100 60 1 26 124 31 130 4.85e-12 62.0
Msa0723140 AT3G28080.3 51.064 94 45 1 34 126 33 126 4.95e-12 61.6
Msa0723140 AT3G28080.1 51.064 94 45 1 34 126 33 126 4.95e-12 62.0
Msa0723140 AT5G45370.2 39.000 100 60 1 26 124 31 130 5.66e-12 61.6
Msa0723140 AT5G45370.4 39.000 100 60 1 26 124 31 130 5.66e-12 61.6
Msa0723140 AT3G28130.6 44.681 94 51 1 34 126 32 125 6.59e-12 60.5
Msa0723140 AT3G53210.3 32.710 107 71 1 17 122 12 118 9.12e-12 60.8
Msa0723140 AT3G28130.4 44.681 94 51 1 34 126 32 125 1.21e-11 60.5
Msa0723140 AT3G53210.1 32.710 107 71 1 17 122 12 118 1.88e-11 60.1
Msa0723140 AT3G28130.2 44.681 94 51 1 34 126 32 125 1.90e-11 60.1
Msa0723140 AT1G25270.1 33.663 101 66 1 27 126 15 115 7.21e-11 58.5

Find 105 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAAATTTGAATTGGATTT+TGG 0.037445 5_1:+20772440 Msa0723140:three_prime_UTR
TTTATAAATTGTTCAATATT+TGG 0.130770 5_1:-20771456 None:intergenic
CTTCAAAGTTCAACTATTAT+TGG 0.135061 5_1:-20771147 None:intergenic
CCCAAAATCCAATTCAAATT+TGG 0.154023 5_1:-20772441 None:intergenic
CTCAATATGTAAATCAATAT+TGG 0.178556 5_1:+20771347 Msa0723140:three_prime_UTR
AGAGACATCATTATTAAATT+TGG 0.199198 5_1:+20771906 Msa0723140:three_prime_UTR
AAAATGGATTTCAGGTAAAA+TGG 0.211382 5_1:+20770701 Msa0723140:three_prime_UTR
TCTTTGATACTTTATTTAAA+AGG 0.214893 5_1:+20771785 Msa0723140:three_prime_UTR
CTCAATTTAATGGTTTAATT+AGG 0.229946 5_1:+20772265 Msa0723140:three_prime_UTR
TATTTGTGAAATGAAGATTT+TGG 0.257344 5_1:+20770826 Msa0723140:three_prime_UTR
CTTAGTCGTGATATTGGAAT+TGG 0.268624 5_1:-20771684 None:intergenic
CATAAAAGCAAGGTAAATTA+TGG 0.269601 5_1:-20771378 None:intergenic
TTGATCTCTAACCACAATTA+AGG 0.270039 5_1:-20772705 None:intergenic
ATTCCTCTTAGTCGTGATAT+TGG 0.276002 5_1:-20771690 None:intergenic
TACTATAGTTCCTATTGCTT+TGG 0.311918 5_1:-20772325 None:intergenic
TTAGGATTGAAAGAATATAT+TGG 0.312093 5_1:+20772283 Msa0723140:three_prime_UTR
TACATAAAGTGTAAATTATT+AGG 0.321962 5_1:-20771222 None:intergenic
GAATAATAATATGTAATAGA+AGG 0.334076 5_1:-20771277 None:intergenic
TTGTCTTACCTGAAAACAAT+AGG 0.341324 5_1:+20770799 Msa0723140:three_prime_UTR
TTTGAAGTTAATTAAATGTT+GGG 0.341414 5_1:+20771163 Msa0723140:three_prime_UTR
TTCAATGTGGTACATATATC+AGG 0.347163 5_1:+20772497 Msa0723140:three_prime_UTR
TTTATTCATTCCATCTTTCA+TGG 0.347498 5_1:-20769864 None:intergenic
CCTTAGCTTTGTTTATCAAC+AGG 0.352160 5_1:+20769936 Msa0723140:CDS
ATATAAATTAATAATAGTTA+TGG 0.352337 5_1:-20774475 None:intergenic
ATGGGAACTAAAGCTTGATA+TGG 0.364178 5_1:+20772733 Msa0723140:three_prime_UTR
TTTATTGATTAAAATTCATT+CGG 0.370611 5_1:-20770730 None:intergenic
ATAGTTATGGGTTCTTGTTA+AGG 0.381892 5_1:-20774462 None:intergenic
TCAATATGTAAATCAATATT+GGG 0.390670 5_1:+20771348 Msa0723140:three_prime_UTR
TATAAATTAATAATAGTTAT+GGG 0.391104 5_1:-20774474 None:intergenic
TAACAGGCAATAGCTGCAAT+AGG 0.395737 5_1:+20773970 Msa0723140:intron
CCAAAGTGCAGAGAAAGTTA+AGG 0.408072 5_1:-20770120 None:intergenic
CTTTGAAGTTAATTAAATGT+TGG 0.409614 5_1:+20771162 Msa0723140:three_prime_UTR
ATGACTCTTTGCAGTTTGAT+TGG 0.423275 5_1:+20774234 Msa0723140:intron
TGTAATGGTTGTTTATTCCA+TGG 0.425563 5_1:+20769889 Msa0723140:CDS
TCAGCACAGATAATGACTTA+TGG 0.428918 5_1:+20770471 Msa0723140:CDS
GATATCACCACTCCAATGAT+TGG 0.431368 5_1:-20771654 None:intergenic
AGTAGCCAAGCCAAAGCAAT+AGG 0.431540 5_1:+20772315 Msa0723140:three_prime_UTR
TTATTGATTAAAATTCATTC+GGG 0.432159 5_1:-20770729 None:intergenic
CCTTAACTTTCTCTGCACTT+TGG 0.436623 5_1:+20770120 Msa0723140:CDS
TGGGAACTAAAGCTTGATAT+GGG 0.448002 5_1:+20772734 Msa0723140:three_prime_UTR
AATAAACTAAACAATTTGAA+TGG 0.449800 5_1:-20771759 None:intergenic
GCAGAGAAAGAGAGAGATGG+TGG 0.450004 5_1:+20769742 Msa0723140:exon
CGAAGCTTTGCCCCATTGCA+CGG 0.455349 5_1:-20774286 None:intergenic
ATTAAAGTTGCCATGAAAGA+TGG 0.461241 5_1:+20769854 Msa0723140:CDS
GATATAATCATTCCCACAAA+AGG 0.462366 5_1:-20769800 None:intergenic
ATACTCTAGATTACAATTTG+TGG 0.462425 5_1:+20771810 Msa0723140:three_prime_UTR
CGTGCAATGGGGCAAAGCTT+CGG 0.467000 5_1:+20774287 Msa0723140:three_prime_UTR
AATAGGAACTATAGTATCAA+TGG 0.467878 5_1:+20772332 Msa0723140:three_prime_UTR
GATGAATGTATCTTGAAGAT+TGG 0.472697 5_1:-20772390 None:intergenic
TAGTTCCTATTGCTTTGGCT+TGG 0.473979 5_1:-20772320 None:intergenic
ACAAAACAGCATAGAGAGAG+AGG 0.474981 5_1:+20769718 Msa0723140:five_prime_UTR
GTAATGGTTGTTTATTCCAT+GGG 0.479195 5_1:+20769890 Msa0723140:CDS
ATTTGTTGTCATATTATACA+AGG 0.490744 5_1:+20772362 Msa0723140:three_prime_UTR
CAAAGTGCAGAGAAAGTTAA+GGG 0.497706 5_1:-20770119 None:intergenic
TTTATTAGATAAAACTCGGA+TGG 0.506084 5_1:+20770665 Msa0723140:three_prime_UTR
AACAGGCAATAGCTGCAATA+GGG 0.513949 5_1:+20773971 Msa0723140:intron
AATAATAATATGTAATAGAA+GGG 0.514267 5_1:-20771276 None:intergenic
CCTGTTGATAAACAAAGCTA+AGG 0.521025 5_1:-20769936 None:intergenic
TGTGCTATGTTTAAACCGAT+CGG 0.521072 5_1:+20774045 Msa0723140:three_prime_UTR
TAAATGGAGGCAAAACATCA+AGG 0.525704 5_1:+20772118 Msa0723140:three_prime_UTR
GCAAGTACAAAAGTGAAAGC+TGG 0.527966 5_1:-20770546 None:intergenic
ATTCCAATATCACGACTAAG+AGG 0.530133 5_1:+20771687 Msa0723140:three_prime_UTR
TTAAACATAGCACAAAATAG+AGG 0.532153 5_1:-20774036 None:intergenic
GTACTAAGTACTATTATATG+AGG 0.543759 5_1:+20771189 Msa0723140:three_prime_UTR
TAAGGGAAGAAGCAAGATGC+TGG 0.545226 5_1:-20769918 None:intergenic
CAGAGAAAGAGAGAGATGGT+GGG 0.548363 5_1:+20769743 Msa0723140:exon
TTGTAACAATGGAACAACAT+TGG 0.549077 5_1:-20774352 None:intergenic
TCACAAATATAAATAAAAGA+TGG 0.552234 5_1:+20770883 Msa0723140:three_prime_UTR
AAGACTATGACTATTACAGC+AGG 0.552572 5_1:-20772641 None:intergenic
ACATGGTGCGTGCAAAGAGC+TGG 0.555731 5_1:+20774012 Msa0723140:three_prime_UTR
GAAAGAGAGAGATGGTGGGA+TGG 0.556396 5_1:+20769747 Msa0723140:exon
TTGAAGTTAATTAAATGTTG+GGG 0.562622 5_1:+20771164 Msa0723140:three_prime_UTR
GGGGCAGAGAAAGAGAGAGA+TGG 0.566526 5_1:+20769739 Msa0723140:exon
GGAACTATAGTATCAATGGC+TGG 0.566753 5_1:+20772336 Msa0723140:three_prime_UTR
TCTGAATCCAATCATTGGAG+TGG 0.574334 5_1:+20771647 Msa0723140:three_prime_UTR
GAATGTATCTTGAAGATTGG+TGG 0.575361 5_1:-20772387 None:intergenic
CAAAACAGCATAGAGAGAGA+GGG 0.578034 5_1:+20769719 Msa0723140:five_prime_UTR
CAAAAGTGAAAGCTGGAATG+AGG 0.578615 5_1:-20770539 None:intergenic
TGGTTAGAGATCAAAGTGCA+TGG 0.579684 5_1:+20772714 Msa0723140:three_prime_UTR
ATTCTTTACTTTCTTCAATG+TGG 0.587667 5_1:+20772484 Msa0723140:three_prime_UTR
CTTGATTCTATGTGATGCCA+TGG 0.589059 5_1:+20772146 Msa0723140:three_prime_UTR
TGGAATGAATAAATATGTAA+TGG 0.590938 5_1:+20769874 Msa0723140:CDS
GGGAGTATATGCCTTAATTG+TGG 0.595399 5_1:+20772694 Msa0723140:three_prime_UTR
TTTAGCTCAAAGTGGAAGCA+TGG 0.596575 5_1:+20769826 Msa0723140:CDS
AAAACAGCATAGAGAGAGAG+GGG 0.599540 5_1:+20769720 Msa0723140:five_prime_UTR
TTGTATCACATCTAAAGACA+AGG 0.604246 5_1:-20771116 None:intergenic
CAAGATGCTGGAAAGTCCCA+TGG 0.607920 5_1:-20769906 None:intergenic
TTCGATACTTGTTACAAACA+TGG 0.608790 5_1:+20773995 Msa0723140:three_prime_UTR
GTGGGATGGCTGAACTTGAA+TGG 0.611210 5_1:+20769761 Msa0723140:CDS
ACGGTGAAGATTATTCCGAT+CGG 0.613970 5_1:-20774060 None:intergenic
ATTATCATATTACTAGACCA+TGG 0.615349 5_1:-20772163 None:intergenic
GGTGCTGTAATAATCGTGGT+AGG 0.620799 5_1:+20774255 Msa0723140:three_prime_UTR
TTTGTTGTCATATTATACAA+GGG 0.622068 5_1:+20772363 Msa0723140:three_prime_UTR
TGTATCACATCTAAAGACAA+GGG 0.630488 5_1:-20771115 None:intergenic
GGTTAGAGATCAAAGTGCAT+GGG 0.639263 5_1:+20772715 Msa0723140:three_prime_UTR
AAGTTAATTAAATGTTGGGG+CGG 0.647467 5_1:+20771167 Msa0723140:three_prime_UTR
CTGTTGATAAACAAAGCTAA+GGG 0.652776 5_1:-20769935 None:intergenic
ATAACATATGTACAAAGTCG+TGG 0.654026 5_1:-20769686 None:intergenic
GCACAGATAATGACTTATGG+TGG 0.656302 5_1:+20770474 Msa0723140:CDS
GATTGGTGCTGTAATAATCG+TGG 0.671111 5_1:+20774251 Msa0723140:three_prime_UTR
AGCATTGCAGATGCAAGAGT+AGG 0.675175 5_1:-20770513 None:intergenic
AGCTCAAAGTGGAAGCATGG+TGG 0.675538 5_1:+20769829 Msa0723140:CDS
GGGAACTAAAGCTTGATATG+GGG 0.684135 5_1:+20772735 Msa0723140:three_prime_UTR
AGACTATGACTATTACAGCA+GGG 0.696834 5_1:-20772640 None:intergenic
AGTGCAGAGAAAGTTAAGGG+AGG 0.725600 5_1:-20770116 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATTAATAAAATAAAAAAG+GGG - chr5_1:20769964-20769983 None:intergenic 10.0%
!! GAGAATTAATAAAATAAAAA+AGG - chr5_1:20769966-20769985 None:intergenic 10.0%
!!! GTATAATTTTTAAACTTTAT+TGG + chr5_1:20770588-20770607 Msa0723140:exon 10.0%
!!! TTTATTGATTAAAATTCATT+CGG - chr5_1:20770733-20770752 None:intergenic 10.0%
!! AATAATAATATGTAATAGAA+GGG - chr5_1:20771279-20771298 None:intergenic 10.0%
!! TTTATAAATTGTTCAATATT+TGG - chr5_1:20771459-20771478 None:intergenic 10.0%
!! AAAAAAAAATCTCAATTTAA+TGG + chr5_1:20772255-20772274 Msa0723140:three_prime_UTR 10.0%
!!! TTTTTTTGTTTAATTTTCCT+CGG + chr5_1:20770059-20770078 Msa0723140:intron 15.0%
!! TAAAGTTTAAAAATTATACG+AGG - chr5_1:20770587-20770606 None:intergenic 15.0%
!!! AATTTTTAAACTTTATTGGA+AGG + chr5_1:20770592-20770611 Msa0723140:exon 15.0%
!!! TTCTTTTATTAGATAAAACT+CGG + chr5_1:20770661-20770680 Msa0723140:three_prime_UTR 15.0%
!!! TTATTGATTAAAATTCATTC+GGG - chr5_1:20770732-20770751 None:intergenic 15.0%
!! TCACAAATATAAATAAAAGA+TGG + chr5_1:20770883-20770902 Msa0723140:three_prime_UTR 15.0%
!!! TTTTTCATTTGTATTTGTAT+TGG + chr5_1:20771007-20771026 Msa0723140:three_prime_UTR 15.0%
!!! TTTGAAGTTAATTAAATGTT+GGG + chr5_1:20771163-20771182 Msa0723140:three_prime_UTR 15.0%
!! TACATAAAGTGTAAATTATT+AGG - chr5_1:20771225-20771244 None:intergenic 15.0%
!! GAATAATAATATGTAATAGA+AGG - chr5_1:20771280-20771299 None:intergenic 15.0%
!! TCAATATGTAAATCAATATT+GGG + chr5_1:20771348-20771367 Msa0723140:three_prime_UTR 15.0%
!!! AAAATCATATGAATTGTTTT+TGG - chr5_1:20771488-20771507 None:intergenic 15.0%
!! ACAAAAAAAAAATGTTAATC+TGG - chr5_1:20771537-20771556 None:intergenic 15.0%
!!! AGATTAACATTTTTTTTTTG+TGG + chr5_1:20771536-20771555 Msa0723140:three_prime_UTR 15.0%
!!! CAGAAAAACAAATTTTATAT+TGG - chr5_1:20771631-20771650 None:intergenic 15.0%
!! AATAAACTAAACAATTTGAA+TGG - chr5_1:20771762-20771781 None:intergenic 15.0%
!!! TCTTTGATACTTTATTTAAA+AGG + chr5_1:20771785-20771804 Msa0723140:three_prime_UTR 15.0%
!!! ATTTTTAATAATGATGTATG+AGG - chr5_1:20771934-20771953 None:intergenic 15.0%
!!! ATTATTTTGTGATTTTTGTT+GGG - chr5_1:20772057-20772076 None:intergenic 15.0%
!!! AATTATTTTGTGATTTTTGT+TGG - chr5_1:20772058-20772077 None:intergenic 15.0%
!!! TTTATAAAACTTTTAAATGG+AGG + chr5_1:20772105-20772124 Msa0723140:three_prime_UTR 15.0%
!! TCTATAAACATGAAATTTAT+TGG + chr5_1:20772800-20772819 Msa0723140:intron 15.0%
!! CTATAAACATGAAATTTATT+GGG + chr5_1:20772801-20772820 Msa0723140:intron 15.0%
!!! TTTTTTTTTTTGTACACTAT+TGG + chr5_1:20772828-20772847 Msa0723140:intron 15.0%
!!! TTTTTTTTTTGTACACTATT+GGG + chr5_1:20772829-20772848 Msa0723140:intron 15.0%
!! AAAAAAAAAATGATTATGAC+AGG + chr5_1:20773180-20773199 Msa0723140:intron 15.0%
!!! AACAAATTATCATTTTTCTT+CGG - chr5_1:20773669-20773688 None:intergenic 15.0%
!! TGGAATGAATAAATATGTAA+TGG + chr5_1:20769874-20769893 Msa0723140:CDS 20.0%
!!! AAATATGATATGTTTTGAGT+GGG - chr5_1:20770251-20770270 None:intergenic 20.0%
!!! TAAATATGATATGTTTTGAG+TGG - chr5_1:20770252-20770271 None:intergenic 20.0%
!!! ATTTTTTGTCACTTACTAAA+AGG - chr5_1:20770326-20770345 None:intergenic 20.0%
!! AATAATTTAATCCAAGAGAA+AGG - chr5_1:20770436-20770455 None:intergenic 20.0%
!!! GAAATCCATTTTTAAAATGT+GGG - chr5_1:20770693-20770712 None:intergenic 20.0%
!!! TGAAATCCATTTTTAAAATG+TGG - chr5_1:20770694-20770713 None:intergenic 20.0%
!!! ACATTTTAAAAATGGATTTC+AGG + chr5_1:20770693-20770712 Msa0723140:three_prime_UTR 20.0%
!!! AATAAAATCCTATTGTTTTC+AGG - chr5_1:20770810-20770829 None:intergenic 20.0%
!!! TATTTGTGAAATGAAGATTT+TGG + chr5_1:20770826-20770845 Msa0723140:three_prime_UTR 20.0%
!! TGAAAAAAGTTTAGACATAA+TGG - chr5_1:20770994-20771013 None:intergenic 20.0%
!!! CTTTGAAGTTAATTAAATGT+TGG + chr5_1:20771162-20771181 Msa0723140:three_prime_UTR 20.0%
!! TTGAAGTTAATTAAATGTTG+GGG + chr5_1:20771164-20771183 Msa0723140:three_prime_UTR 20.0%
!! CTCAATATGTAAATCAATAT+TGG + chr5_1:20771347-20771366 Msa0723140:three_prime_UTR 20.0%
!! AAAAAAATCTCATAAAAGCA+AGG - chr5_1:20771391-20771410 None:intergenic 20.0%
!! AGAGACATCATTATTAAATT+TGG + chr5_1:20771906-20771925 Msa0723140:three_prime_UTR 20.0%
!! GAATTATCTCAAAAATTGTT+GGG - chr5_1:20771966-20771985 None:intergenic 20.0%
!! AGAATTATCTCAAAAATTGT+TGG - chr5_1:20771967-20771986 None:intergenic 20.0%
!! CTCAATTTAATGGTTTAATT+AGG + chr5_1:20772265-20772284 Msa0723140:three_prime_UTR 20.0%
!! TTAGGATTGAAAGAATATAT+TGG + chr5_1:20772283-20772302 Msa0723140:three_prime_UTR 20.0%
!!! ATTTGTTGTCATATTATACA+AGG + chr5_1:20772362-20772381 Msa0723140:three_prime_UTR 20.0%
!!! TTTGTTGTCATATTATACAA+GGG + chr5_1:20772363-20772382 Msa0723140:three_prime_UTR 20.0%
!!! AATTTTCACCAAATTTGAAT+TGG + chr5_1:20772433-20772452 Msa0723140:three_prime_UTR 20.0%
!!! TACACTTTTTACCATAATAT+GGG + chr5_1:20772523-20772542 Msa0723140:intron 20.0%
!! ACATATTACTCAAAAGAAAA+AGG - chr5_1:20772550-20772569 None:intergenic 20.0%
!!! GTAATATGTTTTTTACCATA+AGG + chr5_1:20772561-20772580 Msa0723140:intron 20.0%
!! CCAAAAATTAATTTACCTTA+TGG - chr5_1:20772579-20772598 None:intergenic 20.0%
!!! CCATAAGGTAAATTAATTTT+TGG + chr5_1:20772576-20772595 Msa0723140:intron 20.0%
!!! CTTTTTTATCACAATTCAAA+CGG + chr5_1:20772673-20772692 Msa0723140:three_prime_UTR 20.0%
!!! TTTTTTATCACAATTCAAAC+GGG + chr5_1:20772674-20772693 Msa0723140:three_prime_UTR 20.0%
!! ATAATAAATGAATGATTACC+TGG - chr5_1:20772778-20772797 None:intergenic 20.0%
!!! TTTTCTCTCTTTATTGTTTT+AGG + chr5_1:20772957-20772976 Msa0723140:intron 20.0%
!!! AGATTATTTGAGCAATTTTT+CGG - chr5_1:20773040-20773059 None:intergenic 20.0%
!! TTTCAAACTACTAAACAAAA+TGG + chr5_1:20773067-20773086 Msa0723140:intron 20.0%
!! AATAAAAATCATTCAACTTG+TGG + chr5_1:20773304-20773323 Msa0723140:intron 20.0%
!!! TCATAAATCATTATTTTCTG+TGG - chr5_1:20773364-20773383 None:intergenic 20.0%
!! AAAATGTAAGCTTAATAATG+TGG - chr5_1:20773435-20773454 None:intergenic 20.0%
!! AAAAATTGAACCCAAAAAAA+CGG - chr5_1:20774082-20774101 None:intergenic 20.0%
!!! TATACTTTGTTTTGTAACAA+TGG - chr5_1:20774366-20774385 None:intergenic 20.0%
!! AAGCAAATTATTACCTTTTG+TGG + chr5_1:20769787-20769806 Msa0723140:CDS 25.0%
!! AGCAAATTATTACCTTTTGT+GGG + chr5_1:20769788-20769807 Msa0723140:CDS 25.0%
! TTTATTCATTCCATCTTTCA+TGG - chr5_1:20769867-20769886 None:intergenic 25.0%
!! TTATTGGAAGGTGAATTTTA+TGG + chr5_1:20770604-20770623 Msa0723140:three_prime_UTR 25.0%
! TTTATTAGATAAAACTCGGA+TGG + chr5_1:20770665-20770684 Msa0723140:three_prime_UTR 25.0%
!! AAAATGGATTTCAGGTAAAA+TGG + chr5_1:20770701-20770720 Msa0723140:three_prime_UTR 25.0%
! CTTCAAAGTTCAACTATTAT+TGG - chr5_1:20771150-20771169 None:intergenic 25.0%
! GTACTAAGTACTATTATATG+AGG + chr5_1:20771189-20771208 Msa0723140:three_prime_UTR 25.0%
! CATAAAAGCAAGGTAAATTA+TGG - chr5_1:20771381-20771400 None:intergenic 25.0%
! GTTAATCTGGTTAAAAAAGA+GGG - chr5_1:20771524-20771543 None:intergenic 25.0%
! TGTTAATCTGGTTAAAAAAG+AGG - chr5_1:20771525-20771544 None:intergenic 25.0%
!!! TTTTTGTGGTTTTATATGCA+TGG + chr5_1:20771550-20771569 Msa0723140:three_prime_UTR 25.0%
! ATACTCTAGATTACAATTTG+TGG + chr5_1:20771810-20771829 Msa0723140:three_prime_UTR 25.0%
! TAAAAAGAGAGAAAAGAAGA+AGG + chr5_1:20772027-20772046 Msa0723140:three_prime_UTR 25.0%
! ATTATCATATTACTAGACCA+TGG - chr5_1:20772166-20772185 None:intergenic 25.0%
! AATAGGAACTATAGTATCAA+TGG + chr5_1:20772332-20772351 Msa0723140:three_prime_UTR 25.0%
!! ACCAAATTTGAATTGGATTT+TGG + chr5_1:20772440-20772459 Msa0723140:three_prime_UTR 25.0%
!! CCAAATTTGAATTGGATTTT+GGG + chr5_1:20772441-20772460 Msa0723140:three_prime_UTR 25.0%
! ATTCTTTACTTTCTTCAATG+TGG + chr5_1:20772484-20772503 Msa0723140:three_prime_UTR 25.0%
!! CATATTATGGTAAAAAGTGT+AGG - chr5_1:20772524-20772543 None:intergenic 25.0%
!!! CTACACTTTTTACCATAATA+TGG + chr5_1:20772522-20772541 Msa0723140:intron 25.0%
! GAATTGTGATAAAAAAGACT+TGG - chr5_1:20772670-20772689 None:intergenic 25.0%
! AAACATGAAATTTATTGGGT+AGG + chr5_1:20772805-20772824 Msa0723140:intron 25.0%
! TGTTTAGTAGTTTGAAAAAG+AGG - chr5_1:20773064-20773083 None:intergenic 25.0%
!!! TATTTAGAGAGAATTTGTTG+AGG + chr5_1:20773115-20773134 Msa0723140:intron 25.0%
!!! ATTTAGAGAGAATTTGTTGA+GGG + chr5_1:20773116-20773135 Msa0723140:intron 25.0%
! TATGATATATACTTGTAGAG+AGG + chr5_1:20773141-20773160 Msa0723140:intron 25.0%
! ATGATATATACTTGTAGAGA+GGG + chr5_1:20773142-20773161 Msa0723140:intron 25.0%
! AAATGATTATGACAGGAATT+TGG + chr5_1:20773187-20773206 Msa0723140:intron 25.0%
! TTAAACATAGCACAAAATAG+AGG - chr5_1:20774039-20774058 None:intergenic 25.0%
!!! AATAATCTTCACCGTTTTTT+TGG + chr5_1:20774068-20774087 Msa0723140:three_prime_UTR 25.0%
!!! ATAATCTTCACCGTTTTTTT+GGG + chr5_1:20774069-20774088 Msa0723140:three_prime_UTR 25.0%
!!! TTTTTGGGTTCAATTTTTCT+AGG + chr5_1:20774084-20774103 Msa0723140:three_prime_UTR 25.0%
ATAACATATGTACAAAGTCG+TGG - chr5_1:20769689-20769708 None:intergenic 30.0%
GATATAATCATTCCCACAAA+AGG - chr5_1:20769803-20769822 None:intergenic 30.0%
!!! ATATCTGTTTTAGCTCAAAG+TGG + chr5_1:20769818-20769837 Msa0723140:CDS 30.0%
ATTAAAGTTGCCATGAAAGA+TGG + chr5_1:20769854-20769873 Msa0723140:CDS 30.0%
TGTAATGGTTGTTTATTCCA+TGG + chr5_1:20769889-20769908 Msa0723140:CDS 30.0%
GTAATGGTTGTTTATTCCAT+GGG + chr5_1:20769890-20769909 Msa0723140:CDS 30.0%
! CTGTTGATAAACAAAGCTAA+GGG - chr5_1:20769938-20769957 None:intergenic 30.0%
TGCCAATAAAAGAAGAGAAA+AGG - chr5_1:20770085-20770104 None:intergenic 30.0%
! TTTTCTCTTCTTTTATTGGC+AGG + chr5_1:20770084-20770103 Msa0723140:intron 30.0%
!! TGGATCCCACATTTTAAAAA+TGG + chr5_1:20770685-20770704 Msa0723140:three_prime_UTR 30.0%
TTGTCTTACCTGAAAACAAT+AGG + chr5_1:20770799-20770818 Msa0723140:three_prime_UTR 30.0%
!!! AAGATTTTGGAGCATATCAT+CGG + chr5_1:20770839-20770858 Msa0723140:three_prime_UTR 30.0%
TGTATCACATCTAAAGACAA+GGG - chr5_1:20771118-20771137 None:intergenic 30.0%
TTGTATCACATCTAAAGACA+AGG - chr5_1:20771119-20771138 None:intergenic 30.0%
AAGTTAATTAAATGTTGGGG+CGG + chr5_1:20771167-20771186 Msa0723140:three_prime_UTR 30.0%
! GTTTTTCTGAATCCAATCAT+TGG + chr5_1:20771642-20771661 Msa0723140:three_prime_UTR 30.0%
TACTATAGTTCCTATTGCTT+TGG - chr5_1:20772328-20772347 None:intergenic 30.0%
GATGAATGTATCTTGAAGAT+TGG - chr5_1:20772393-20772412 None:intergenic 30.0%
CCCAAAATCCAATTCAAATT+TGG - chr5_1:20772444-20772463 None:intergenic 30.0%
! AATTTGAATTGGATTTTGGG+AGG + chr5_1:20772444-20772463 Msa0723140:three_prime_UTR 30.0%
!! ATTTGAATTGGATTTTGGGA+GGG + chr5_1:20772445-20772464 Msa0723140:three_prime_UTR 30.0%
TTCAATGTGGTACATATATC+AGG + chr5_1:20772497-20772516 Msa0723140:three_prime_UTR 30.0%
AAGAAAAAGGTCCCATATTA+TGG - chr5_1:20772537-20772556 None:intergenic 30.0%
TGAAACCTAAAATATGTAGC+GGG - chr5_1:20772606-20772625 None:intergenic 30.0%
CTGAAACCTAAAATATGTAG+CGG - chr5_1:20772607-20772626 None:intergenic 30.0%
TTGATCTCTAACCACAATTA+AGG - chr5_1:20772708-20772727 None:intergenic 30.0%
!!! GTTGATTGTCATTTTATACC+AGG + chr5_1:20772757-20772776 Msa0723140:three_prime_UTR 30.0%
!!! TTTTTTGTACACTATTGGGA+TGG + chr5_1:20772833-20772852 Msa0723140:intron 30.0%
CTACTAAACAAAATGGAACA+CGG + chr5_1:20773074-20773093 Msa0723140:intron 30.0%
!! TTTAGAGAGAATTTGTTGAG+GGG + chr5_1:20773117-20773136 Msa0723140:intron 30.0%
TGATATATACTTGTAGAGAG+GGG + chr5_1:20773143-20773162 Msa0723140:intron 30.0%
! CCTTATAACTAATGGACAAA+TGG - chr5_1:20773394-20773413 None:intergenic 30.0%
CCATTTGTCCATTAGTTATA+AGG + chr5_1:20773391-20773410 Msa0723140:intron 30.0%
!! AATGATAATTTGTTCGCTCA+AGG + chr5_1:20773675-20773694 Msa0723140:intron 30.0%
TTAAAAAATAGTACGGTACG+AGG - chr5_1:20773816-20773835 None:intergenic 30.0%
TGTTAACAGGGATATATAAG+AGG - chr5_1:20773955-20773974 None:intergenic 30.0%
TCTTATATATCCCTGTTAAC+AGG + chr5_1:20773954-20773973 Msa0723140:intron 30.0%
! TTCGATACTTGTTACAAACA+TGG + chr5_1:20773995-20774014 Msa0723140:three_prime_UTR 30.0%
TTGTAACAATGGAACAACAT+TGG - chr5_1:20774355-20774374 None:intergenic 30.0%
ATAGTTATGGGTTCTTGTTA+AGG - chr5_1:20774465-20774484 None:intergenic 30.0%
! CCTGTTGATAAACAAAGCTA+AGG - chr5_1:20769939-20769958 None:intergenic 35.0%
!! CCTTAGCTTTGTTTATCAAC+AGG + chr5_1:20769936-20769955 Msa0723140:CDS 35.0%
! GGCCTTTTCTCTTCTTTTAT+TGG + chr5_1:20770080-20770099 Msa0723140:intron 35.0%
CAAAGTGCAGAGAAAGTTAA+GGG - chr5_1:20770122-20770141 None:intergenic 35.0%
! TTCTTTTTGCTTGCTCTCAT+AGG + chr5_1:20770146-20770165 Msa0723140:CDS 35.0%
! TCTTTTTGCTTGCTCTCATA+GGG + chr5_1:20770147-20770166 Msa0723140:CDS 35.0%
!! TATGATATGTTTTGAGTGGG+TGG - chr5_1:20770248-20770267 None:intergenic 35.0%
GAATAATGACCTTTACCTCA+GGG - chr5_1:20770304-20770323 None:intergenic 35.0%
TTCCAAAACTTCCTTTCTCT+TGG + chr5_1:20770422-20770441 Msa0723140:intron 35.0%
! ATCCAAGAGAAAGGAAGTTT+TGG - chr5_1:20770427-20770446 None:intergenic 35.0%
TCAGCACAGATAATGACTTA+TGG + chr5_1:20770471-20770490 Msa0723140:CDS 35.0%
!!! TTGTACTTGCACTGATTTTC+AGG + chr5_1:20770559-20770578 Msa0723140:CDS 35.0%
CTTAGTCGTGATATTGGAAT+TGG - chr5_1:20771687-20771706 None:intergenic 35.0%
ATTCCAATATCACGACTAAG+AGG + chr5_1:20771687-20771706 Msa0723140:three_prime_UTR 35.0%
ATTCCTCTTAGTCGTGATAT+TGG - chr5_1:20771693-20771712 None:intergenic 35.0%
TAAATGGAGGCAAAACATCA+AGG + chr5_1:20772118-20772137 Msa0723140:three_prime_UTR 35.0%
GAATGTATCTTGAAGATTGG+TGG - chr5_1:20772390-20772409 None:intergenic 35.0%
! GTTTTCCCGCTACATATTTT+AGG + chr5_1:20772598-20772617 Msa0723140:intron 35.0%
AGACTATGACTATTACAGCA+GGG - chr5_1:20772643-20772662 None:intergenic 35.0%
AAGACTATGACTATTACAGC+AGG - chr5_1:20772644-20772663 None:intergenic 35.0%
ATGGGAACTAAAGCTTGATA+TGG + chr5_1:20772733-20772752 Msa0723140:three_prime_UTR 35.0%
TGGGAACTAAAGCTTGATAT+GGG + chr5_1:20772734-20772753 Msa0723140:three_prime_UTR 35.0%
GATACTCGCAATTTCTTGAA+AGG - chr5_1:20773270-20773289 None:intergenic 35.0%
TGTGGTATTCCTTCTTATCT+TGG + chr5_1:20773322-20773341 Msa0723140:intron 35.0%
TTATGTGTGCCAAGATAAGA+AGG - chr5_1:20773334-20773353 None:intergenic 35.0%
TGTCCATTAGTTATAAGGAC+CGG + chr5_1:20773396-20773415 Msa0723140:intron 35.0%
TGTGCTATGTTTAAACCGAT+CGG + chr5_1:20774045-20774064 Msa0723140:three_prime_UTR 35.0%
! CTAGGAGATGACTTTTATCT+TGG + chr5_1:20774102-20774121 Msa0723140:three_prime_UTR 35.0%
! GAGATGACTTTTATCTTGGA+AGG + chr5_1:20774106-20774125 Msa0723140:three_prime_UTR 35.0%
ATGACTCTTTGCAGTTTGAT+TGG + chr5_1:20774234-20774253 Msa0723140:intron 35.0%
AAAGCTTCGGAAGAAAAAGT+TGG + chr5_1:20774300-20774319 Msa0723140:three_prime_UTR 35.0%
AAGCTTCGGAAGAAAAAGTT+GGG + chr5_1:20774301-20774320 Msa0723140:three_prime_UTR 35.0%
ACAAAACAGCATAGAGAGAG+AGG + chr5_1:20769718-20769737 Msa0723140:five_prime_UTR 40.0%
CAAAACAGCATAGAGAGAGA+GGG + chr5_1:20769719-20769738 Msa0723140:five_prime_UTR 40.0%
AAAACAGCATAGAGAGAGAG+GGG + chr5_1:20769720-20769739 Msa0723140:five_prime_UTR 40.0%
! TTTAGCTCAAAGTGGAAGCA+TGG + chr5_1:20769826-20769845 Msa0723140:CDS 40.0%
TAAAAGAAGAGAAAAGGCCG+AGG - chr5_1:20770079-20770098 None:intergenic 40.0%
CCAAAGTGCAGAGAAAGTTA+AGG - chr5_1:20770123-20770142 None:intergenic 40.0%
CCTTAACTTTCTCTGCACTT+TGG + chr5_1:20770120-20770139 Msa0723140:CDS 40.0%
GGAATAATGACCTTTACCTC+AGG - chr5_1:20770305-20770324 None:intergenic 40.0%
GCACAGATAATGACTTATGG+TGG + chr5_1:20770474-20770493 Msa0723140:CDS 40.0%
CAAAAGTGAAAGCTGGAATG+AGG - chr5_1:20770542-20770561 None:intergenic 40.0%
GCAAGTACAAAAGTGAAAGC+TGG - chr5_1:20770549-20770568 None:intergenic 40.0%
TCTGAATCCAATCATTGGAG+TGG + chr5_1:20771647-20771666 Msa0723140:three_prime_UTR 40.0%
GATATCACCACTCCAATGAT+TGG - chr5_1:20771657-20771676 None:intergenic 40.0%
CTTGATTCTATGTGATGCCA+TGG + chr5_1:20772146-20772165 Msa0723140:three_prime_UTR 40.0%
TAGTTCCTATTGCTTTGGCT+TGG - chr5_1:20772323-20772342 None:intergenic 40.0%
GGAACTATAGTATCAATGGC+TGG + chr5_1:20772336-20772355 Msa0723140:three_prime_UTR 40.0%
! GGGAGTATATGCCTTAATTG+TGG + chr5_1:20772694-20772713 Msa0723140:three_prime_UTR 40.0%
TGGTTAGAGATCAAAGTGCA+TGG + chr5_1:20772714-20772733 Msa0723140:three_prime_UTR 40.0%
GGTTAGAGATCAAAGTGCAT+GGG + chr5_1:20772715-20772734 Msa0723140:three_prime_UTR 40.0%
GGGAACTAAAGCTTGATATG+GGG + chr5_1:20772735-20772754 Msa0723140:three_prime_UTR 40.0%
! TTTTCGGTGAGACAACTTGT+TGG - chr5_1:20773024-20773043 None:intergenic 40.0%
! AACACGGCAGTATCTTTTTG+TGG + chr5_1:20773090-20773109 Msa0723140:intron 40.0%
ATATACTTGTAGAGAGGGGT+AGG + chr5_1:20773147-20773166 Msa0723140:intron 40.0%
TATACTTGTAGAGAGGGGTA+GGG + chr5_1:20773148-20773167 Msa0723140:intron 40.0%
AATTGCGAGTATCTCTGTCA+AGG + chr5_1:20773277-20773296 Msa0723140:intron 40.0%
ATTGCGAGTATCTCTGTCAA+GGG + chr5_1:20773278-20773297 Msa0723140:intron 40.0%
TGTCCGGTCCTTATAACTAA+TGG - chr5_1:20773402-20773421 None:intergenic 40.0%
TAACAGGCAATAGCTGCAAT+AGG + chr5_1:20773970-20773989 Msa0723140:intron 40.0%
AACAGGCAATAGCTGCAATA+GGG + chr5_1:20773971-20773990 Msa0723140:intron 40.0%
ACGGTGAAGATTATTCCGAT+CGG - chr5_1:20774063-20774082 None:intergenic 40.0%
!! GATTGGTGCTGTAATAATCG+TGG + chr5_1:20774251-20774270 Msa0723140:three_prime_UTR 40.0%
! TAGGATTTTATGCCGTGCAA+TGG + chr5_1:20774274-20774293 Msa0723140:three_prime_UTR 40.0%
! AGGATTTTATGCCGTGCAAT+GGG + chr5_1:20774275-20774294 Msa0723140:three_prime_UTR 40.0%
TCGGAAGAAAAAGTTGGGAA+AGG + chr5_1:20774306-20774325 Msa0723140:three_prime_UTR 40.0%
CGGAAGAAAAAGTTGGGAAA+GGG + chr5_1:20774307-20774326 Msa0723140:three_prime_UTR 40.0%
CAGAGAAAGAGAGAGATGGT+GGG + chr5_1:20769743-20769762 Msa0723140:exon 45.0%
TAAGGGAAGAAGCAAGATGC+TGG - chr5_1:20769921-20769940 None:intergenic 45.0%
AGTGCAGAGAAAGTTAAGGG+AGG - chr5_1:20770119-20770138 None:intergenic 45.0%
CCTCAGGGAAAAGTCGATTA+TGG - chr5_1:20770289-20770308 None:intergenic 45.0%
!! CCATAATCGACTTTTCCCTG+AGG + chr5_1:20770286-20770305 Msa0723140:intron 45.0%
! TCGACTTTTCCCTGAGGTAA+AGG + chr5_1:20770292-20770311 Msa0723140:intron 45.0%
ATCTGTGCTGAAGATCTGCA+AGG - chr5_1:20770462-20770481 None:intergenic 45.0%
AGCATTGCAGATGCAAGAGT+AGG - chr5_1:20770516-20770535 None:intergenic 45.0%
AGTAGCCAAGCCAAAGCAAT+AGG + chr5_1:20772315-20772334 Msa0723140:three_prime_UTR 45.0%
! TTGGGAGGGTTTTTATGTGC+TGG + chr5_1:20772459-20772478 Msa0723140:three_prime_UTR 45.0%
TTGCGAGTATCTCTGTCAAG+GGG + chr5_1:20773279-20773298 Msa0723140:intron 45.0%
ATAAGGACCGGACAACATTC+AGG + chr5_1:20773408-20773427 Msa0723140:intron 45.0%
ATGTGGTCCTGAATGTTGTC+CGG - chr5_1:20773418-20773437 None:intergenic 45.0%
GCAGCTATTGCCTGTTAACA+GGG - chr5_1:20773967-20773986 None:intergenic 45.0%
TGCAGCTATTGCCTGTTAAC+AGG - chr5_1:20773968-20773987 None:intergenic 45.0%
! GGTGCTGTAATAATCGTGGT+AGG + chr5_1:20774255-20774274 Msa0723140:three_prime_UTR 45.0%
! GGATTTTATGCCGTGCAATG+GGG + chr5_1:20774276-20774295 Msa0723140:three_prime_UTR 45.0%
!! AGAATTAATAAAATAAAAAA+GGG - chr5_1:20769965-20769984 None:intergenic 5.0%
!!! AAATTTATAAAACTTTTAAA+TGG + chr5_1:20772102-20772121 Msa0723140:three_prime_UTR 5.0%
!! ATAATATACAAAAAAAATTT+AGG - chr5_1:20772995-20773014 None:intergenic 5.0%
!!! TAAATTTTTTTTGTATATTA+TGG + chr5_1:20772994-20773013 Msa0723140:intron 5.0%
!!! CTTTTTTTTTTATAAATTTA+TGG - chr5_1:20773518-20773537 None:intergenic 5.0%
!! TTTAATATTAAAAAATAGTA+CGG - chr5_1:20773823-20773842 None:intergenic 5.0%
GCAGAGAAAGAGAGAGATGG+TGG + chr5_1:20769742-20769761 Msa0723140:exon 50.0%
!! GAAAGAGAGAGATGGTGGGA+TGG + chr5_1:20769747-20769766 Msa0723140:exon 50.0%
GTGGGATGGCTGAACTTGAA+TGG + chr5_1:20769761-20769780 Msa0723140:CDS 50.0%
! AGCTCAAAGTGGAAGCATGG+TGG + chr5_1:20769829-20769848 Msa0723140:CDS 50.0%
CAAGATGCTGGAAAGTCCCA+TGG - chr5_1:20769909-20769928 None:intergenic 50.0%
GGGGCAGAGAAAGAGAGAGA+TGG + chr5_1:20769739-20769758 Msa0723140:exon 55.0%
ACATGGTGCGTGCAAAGAGC+TGG + chr5_1:20774012-20774031 Msa0723140:three_prime_UTR 55.0%
CGAAGCTTTGCCCCATTGCA+CGG - chr5_1:20774289-20774308 None:intergenic 55.0%
CGTGCAATGGGGCAAAGCTT+CGG + chr5_1:20774287-20774306 Msa0723140:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 20769616 20774486 20769616 ID=Msa0723140;Name=Msa0723140
chr5_1 mRNA 20769616 20774486 20769616 ID=Msa0723140-mRNA-1;Parent=Msa0723140;Name=Msa0723140-mRNA-1;_AED=0.04;_eAED=0.04;_QI=142|1|1|1|0.83|0.71|7|2451|129
chr5_1 exon 20769616 20769957 20769616 ID=Msa0723140-mRNA-1:exon:7881;Parent=Msa0723140-mRNA-1
chr5_1 exon 20770106 20770168 20770106 ID=Msa0723140-mRNA-1:exon:7882;Parent=Msa0723140-mRNA-1
chr5_1 exon 20770467 20771578 20770467 ID=Msa0723140-mRNA-1:exon:7883;Parent=Msa0723140-mRNA-1
chr5_1 exon 20771603 20772518 20771603 ID=Msa0723140-mRNA-1:exon:7884;Parent=Msa0723140-mRNA-1
chr5_1 exon 20772620 20772778 20772620 ID=Msa0723140-mRNA-1:exon:7885;Parent=Msa0723140-mRNA-1
chr5_1 exon 20773976 20774127 20773976 ID=Msa0723140-mRNA-1:exon:7886;Parent=Msa0723140-mRNA-1
chr5_1 exon 20774248 20774486 20774248 ID=Msa0723140-mRNA-1:exon:7887;Parent=Msa0723140-mRNA-1
chr5_1 five_prime_UTR 20769616 20769757 20769616 ID=Msa0723140-mRNA-1:five_prime_utr;Parent=Msa0723140-mRNA-1
chr5_1 CDS 20769758 20769957 20769758 ID=Msa0723140-mRNA-1:cds;Parent=Msa0723140-mRNA-1
chr5_1 CDS 20770106 20770168 20770106 ID=Msa0723140-mRNA-1:cds;Parent=Msa0723140-mRNA-1
chr5_1 CDS 20770467 20770593 20770467 ID=Msa0723140-mRNA-1:cds;Parent=Msa0723140-mRNA-1
chr5_1 three_prime_UTR 20770594 20771578 20770594 ID=Msa0723140-mRNA-1:three_prime_utr;Parent=Msa0723140-mRNA-1
chr5_1 three_prime_UTR 20771603 20772518 20771603 ID=Msa0723140-mRNA-1:three_prime_utr;Parent=Msa0723140-mRNA-1
chr5_1 three_prime_UTR 20772620 20772778 20772620 ID=Msa0723140-mRNA-1:three_prime_utr;Parent=Msa0723140-mRNA-1
chr5_1 three_prime_UTR 20773976 20774127 20773976 ID=Msa0723140-mRNA-1:three_prime_utr;Parent=Msa0723140-mRNA-1
chr5_1 three_prime_UTR 20774248 20774486 20774248 ID=Msa0723140-mRNA-1:three_prime_utr;Parent=Msa0723140-mRNA-1
Gene Sequence

>Msa0723140

ATGGTGGGATGGCTGAACTTGAATGGTACAAGCAAATTATTACCTTTTGTGGGAATGATTATATCTGTTTTAGCTCAAAGTGGAAGCATGGTGGTAATTAAAGTTGCCATGAAAGATGGAATGAATAAATATGTAATGGTTGTTTATTCCATGGGACTTTCCAGCATCTTGCTTCTTCCCTTAGCTTTGTTTATCAACAGGTCACAACGTCCTCCCTTAACTTTCTCTGCACTTTGGAGCTTCTTTTTGCTTGCTCTCATAGGATCTTCAGCACAGATAATGACTTATGGTGGCATAGAACTGAGTTCACCTACTCTTGCATCTGCAATGCTTAACCTCATTCCAGCTTTCACTTTTGTACTTGCACTGATTTTCAGGTTCCTCGTATAA
Protein sequence

>Msa0723140

MVGWLNLNGTSKLLPFVGMIISVLAQSGSMVVIKVAMKDGMNKYVMVVYSMGLSSILLLPLALFINRSQRPPLTFSALWSFFLLALIGSSAQIMTYGGIELSSPTLASAMLNLIPAFTFVLALIFRFLV*