Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1454090 | sp|Q8H0Y8|WRK41_ARATH | 43.396 | 159 | 61 | 8 | 8 | 143 | 7 | 159 | 2.87e-22 | 92.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1454090 | G7L427 | 88.889 | 144 | 9 | 1 | 1 | 144 | 1 | 137 | 2.53e-83 | 257 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1454090 | TF | WRKY |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1427810 | Msa1454090 | 0.847592 | 1.049187e-59 | -8.615850e-47 |
| Msa1441200 | Msa1454090 | 0.804404 | 2.196088e-49 | -8.615850e-47 |
| Msa0437330 | Msa1454090 | 0.848693 | 5.209582e-60 | -8.615850e-47 |
| Msa1130350 | Msa1454090 | 0.811008 | 8.680016e-51 | -8.615850e-47 |
| Msa1130630 | Msa1454090 | 0.848757 | 5.001440e-60 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1454090 | MtrunA17_Chr7g0275531 | 88.889 | 144 | 9 | 1 | 1 | 144 | 1 | 137 | 4.87e-87 | 257 |
| Msa1454090 | MtrunA17_Chr8g0347251 | 44.937 | 158 | 68 | 4 | 1 | 143 | 1 | 154 | 6.14e-32 | 115 |
| Msa1454090 | MtrunA17_Chr3g0126391 | 44.595 | 148 | 59 | 9 | 12 | 141 | 7 | 149 | 1.98e-25 | 98.6 |
| Msa1454090 | MtrunA17_Chr8g0387981 | 36.424 | 151 | 85 | 5 | 1 | 142 | 1 | 149 | 2.34e-17 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1454090 | AT4G11070.1 | 43.396 | 159 | 61 | 8 | 8 | 143 | 7 | 159 | 2.92e-23 | 92.8 |
| Msa1454090 | AT5G24110.1 | 40.458 | 131 | 60 | 5 | 14 | 143 | 18 | 131 | 6.99e-20 | 83.6 |
| Msa1454090 | AT4G11070.2 | 39.568 | 139 | 63 | 5 | 8 | 143 | 7 | 127 | 3.86e-19 | 81.3 |
| Msa1454090 | AT4G23810.1 | 33.908 | 174 | 64 | 7 | 11 | 143 | 13 | 176 | 1.59e-18 | 80.1 |
| Msa1454090 | AT2G46400.1 | 38.636 | 132 | 62 | 4 | 12 | 142 | 8 | 121 | 2.84e-13 | 65.5 |
Find 34 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTATGATGACCTCACTTT+TGG | 0.188414 | tig0035033:+672 | None:intergenic |
| TCAACATAGTAAGTGCTTTC+TGG | 0.207919 | tig0035033:+770 | None:intergenic |
| CAAGAATTCATTGGTTTCAT+TGG | 0.239108 | tig0035033:+816 | None:intergenic |
| TTCACTTGTTCCTTCCATTT+AGG | 0.269402 | tig0035033:+315 | None:intergenic |
| CATTGGATGATATAATTATA+TGG | 0.275590 | tig0035033:+833 | None:intergenic |
| AGGATTATCTGAAGGGTCTT+TGG | 0.285031 | tig0035033:-277 | Msa1454090:CDS |
| CTTTCCTTGGATTAATTCAT+TGG | 0.302740 | tig0035033:+879 | None:intergenic |
| GCAGAAAGAGCATGCCTAAA+TGG | 0.338464 | tig0035033:-329 | Msa1454090:intron |
| TATGGTCAAAAGGATATTCT+TGG | 0.360560 | tig0035033:-231 | Msa1454090:CDS |
| TTGTAAAATGATAATGAAGA+TGG | 0.362503 | tig0035033:+942 | None:intergenic |
| GAATATAGCTGGAGAAAATA+TGG | 0.367378 | tig0035033:-249 | Msa1454090:CDS |
| TTTGTCAATCAAGAATTCAT+TGG | 0.407531 | tig0035033:+807 | None:intergenic |
| GCTTTGCTAACTCCTTTCCT+TGG | 0.408135 | tig0035033:+866 | None:intergenic |
| GGACTCTCGTTTGTAAAAGA+AGG | 0.431784 | tig0035033:+694 | None:intergenic |
| AATGAAGATGGGCACTTAAG+TGG | 0.450326 | tig0035033:+954 | None:intergenic |
| CTGGAGAAAATATGGTCAAA+AGG | 0.471109 | tig0035033:-241 | Msa1454090:CDS |
| GGAGAGACTAAGACCATTGA+TGG | 0.480847 | tig0035033:-736 | Msa1454090:CDS |
| GACCATTGATGGTAAAATGA+AGG | 0.490394 | tig0035033:-725 | Msa1454090:CDS |
| TGTAAAATGATAATGAAGAT+GGG | 0.499849 | tig0035033:+943 | None:intergenic |
| ACAATGCTCTCTTCAAGAAA+AGG | 0.501466 | tig0035033:-636 | Msa1454090:intron |
| CTTTGGATGATGAATATAGC+TGG | 0.515960 | tig0035033:-260 | Msa1454090:CDS |
| GAGCATTGTGGTTAAACTCC+TGG | 0.533299 | tig0035033:+650 | None:intergenic |
| CATAGTAAGTGCTTTCTGGT+AGG | 0.575281 | tig0035033:+774 | None:intergenic |
| CAATGAATTAATCCAAGGAA+AGG | 0.585373 | tig0035033:-878 | Msa1454090:CDS |
| CTTACTATGTTGAATGTGAG+AGG | 0.591736 | tig0035033:-760 | Msa1454090:CDS |
| GCACTTAAGTGGAGTGATGA+AGG | 0.598588 | tig0035033:+965 | None:intergenic |
| AAAGAGCATGCCTAAATGGA+AGG | 0.601574 | tig0035033:-325 | Msa1454090:CDS |
| GTGAAGATTTGCACAAACAC+AGG | 0.602509 | tig0035033:-297 | Msa1454090:CDS |
| ATAACCAATGAATTAATCCA+AGG | 0.604601 | tig0035033:-883 | Msa1454090:CDS |
| ACAAACACAGGATTATCTGA+AGG | 0.625241 | tig0035033:-285 | Msa1454090:CDS |
| AAGTGAGGTCATCATAGACC+AGG | 0.627405 | tig0035033:-668 | Msa1454090:CDS |
| CAAACACAGGATTATCTGAA+GGG | 0.645284 | tig0035033:-284 | Msa1454090:CDS |
| AAACGAGAGTCCCAAAAGTG+AGG | 0.654222 | tig0035033:-683 | Msa1454090:CDS |
| ACTATGTTGAATGTGAGAGG+AGG | 0.670945 | tig0035033:-757 | Msa1454090:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATTATCATTTTACAAAAAA+TGG | - | tig0035033:257-276 | Msa1454090:CDS | 15.0% |
| !! | CTATGTTAATAAAAAAGTTA+AGG | + | tig0035033:655-674 | None:intergenic | 15.0% |
| !! | AAATGATACTTGTAATAAAA+CGG | - | tig0035033:732-751 | Msa1454090:CDS | 15.0% |
| !!! | CATTATCATTTTACAAAAAA+TGG | - | tig0035033:257-276 | Msa1454090:CDS | 15.0% |
| !! | CTATGTTAATAAAAAAGTTA+AGG | + | tig0035033:655-674 | None:intergenic | 15.0% |
| !! | AAATGATACTTGTAATAAAA+CGG | - | tig0035033:732-751 | Msa1454090:CDS | 15.0% |
| !!! | TGTAAAATGATAATGAAGAT+GGG | + | tig0035033:252-271 | None:intergenic | 20.0% |
| !!! | TTGTAAAATGATAATGAAGA+TGG | + | tig0035033:253-272 | None:intergenic | 20.0% |
| !! | CATTGGATGATATAATTATA+TGG | + | tig0035033:362-381 | None:intergenic | 20.0% |
| !! | TATGTAATCATTTGAATTTG+TGG | - | tig0035033:839-858 | Msa1454090:CDS | 20.0% |
| !!! | TGTAAAATGATAATGAAGAT+GGG | + | tig0035033:252-271 | None:intergenic | 20.0% |
| !!! | TTGTAAAATGATAATGAAGA+TGG | + | tig0035033:253-272 | None:intergenic | 20.0% |
| !! | CATTGGATGATATAATTATA+TGG | + | tig0035033:362-381 | None:intergenic | 20.0% |
| !! | TATGTAATCATTTGAATTTG+TGG | - | tig0035033:839-858 | Msa1454090:CDS | 20.0% |
| ! | ATAACCAATGAATTAATCCA+AGG | - | tig0035033:309-328 | Msa1454090:CDS | 25.0% |
| ! | TTTGTCAATCAAGAATTCAT+TGG | + | tig0035033:388-407 | None:intergenic | 25.0% |
| ! | ATAACCAATGAATTAATCCA+AGG | - | tig0035033:309-328 | Msa1454090:CDS | 25.0% |
| ! | TTTGTCAATCAAGAATTCAT+TGG | + | tig0035033:388-407 | None:intergenic | 25.0% |
| AAAAAATGGAAGAGACTACA+AGG | - | tig0035033:271-290 | Msa1454090:CDS | 30.0% | |
| CTTTCCTTGGATTAATTCAT+TGG | + | tig0035033:316-335 | None:intergenic | 30.0% | |
| CAATGAATTAATCCAAGGAA+AGG | - | tig0035033:314-333 | Msa1454090:CDS | 30.0% | |
| !! | CAAGAATTCATTGGTTTCAT+TGG | + | tig0035033:379-398 | None:intergenic | 30.0% |
| ! | ATCCTTCATTTTACCATCAA+TGG | + | tig0035033:472-491 | None:intergenic | 30.0% |
| GAATATAGCTGGAGAAAATA+TGG | - | tig0035033:943-962 | Msa1454090:five_prime_UTR | 30.0% | |
| TATGGTCAAAAGGATATTCT+TGG | - | tig0035033:961-980 | Msa1454090:five_prime_UTR | 30.0% | |
| AAAAAATGGAAGAGACTACA+AGG | - | tig0035033:271-290 | Msa1454090:CDS | 30.0% | |
| CTTTCCTTGGATTAATTCAT+TGG | + | tig0035033:316-335 | None:intergenic | 30.0% | |
| CAATGAATTAATCCAAGGAA+AGG | - | tig0035033:314-333 | Msa1454090:CDS | 30.0% | |
| !! | CAAGAATTCATTGGTTTCAT+TGG | + | tig0035033:379-398 | None:intergenic | 30.0% |
| ! | ATCCTTCATTTTACCATCAA+TGG | + | tig0035033:472-491 | None:intergenic | 30.0% |
| GAATATAGCTGGAGAAAATA+TGG | - | tig0035033:943-962 | Msa1454090:five_prime_UTR | 30.0% | |
| TATGGTCAAAAGGATATTCT+TGG | - | tig0035033:961-980 | Msa1454090:five_prime_UTR | 30.0% | |
| TCAACATAGTAAGTGCTTTC+TGG | + | tig0035033:425-444 | None:intergenic | 35.0% | |
| CTTACTATGTTGAATGTGAG+AGG | - | tig0035033:432-451 | Msa1454090:intron | 35.0% | |
| ! | GACCATTGATGGTAAAATGA+AGG | - | tig0035033:467-486 | Msa1454090:intron | 35.0% |
| !! | TCTATGATGACCTCACTTTT+GGG | + | tig0035033:522-541 | None:intergenic | 35.0% |
| !!! | TTTTCTTGAAGAGAGCATTG+TGG | + | tig0035033:557-576 | None:intergenic | 35.0% |
| ACAATGCTCTCTTCAAGAAA+AGG | - | tig0035033:556-575 | Msa1454090:intron | 35.0% | |
| TTCATGTGTGTGAATTGTCA+AGG | - | tig0035033:788-807 | Msa1454090:CDS | 35.0% | |
| ACTGAAATACGTTCAAGCTA+GGG | + | tig0035033:820-839 | None:intergenic | 35.0% | |
| TACTGAAATACGTTCAAGCT+AGG | + | tig0035033:821-840 | None:intergenic | 35.0% | |
| TTCACTTGTTCCTTCCATTT+AGG | + | tig0035033:880-899 | None:intergenic | 35.0% | |
| ACAAACACAGGATTATCTGA+AGG | - | tig0035033:907-926 | Msa1454090:CDS | 35.0% | |
| CAAACACAGGATTATCTGAA+GGG | - | tig0035033:908-927 | Msa1454090:CDS | 35.0% | |
| CTTTGGATGATGAATATAGC+TGG | - | tig0035033:932-951 | Msa1454090:exon | 35.0% | |
| CTGGAGAAAATATGGTCAAA+AGG | - | tig0035033:951-970 | Msa1454090:five_prime_UTR | 35.0% | |
| TCAACATAGTAAGTGCTTTC+TGG | + | tig0035033:425-444 | None:intergenic | 35.0% | |
| CTTACTATGTTGAATGTGAG+AGG | - | tig0035033:432-451 | Msa1454090:intron | 35.0% | |
| ! | GACCATTGATGGTAAAATGA+AGG | - | tig0035033:467-486 | Msa1454090:intron | 35.0% |
| !! | TCTATGATGACCTCACTTTT+GGG | + | tig0035033:522-541 | None:intergenic | 35.0% |
| !!! | TTTTCTTGAAGAGAGCATTG+TGG | + | tig0035033:557-576 | None:intergenic | 35.0% |
| ACAATGCTCTCTTCAAGAAA+AGG | - | tig0035033:556-575 | Msa1454090:intron | 35.0% | |
| TTCATGTGTGTGAATTGTCA+AGG | - | tig0035033:788-807 | Msa1454090:CDS | 35.0% | |
| ACTGAAATACGTTCAAGCTA+GGG | + | tig0035033:820-839 | None:intergenic | 35.0% | |
| TACTGAAATACGTTCAAGCT+AGG | + | tig0035033:821-840 | None:intergenic | 35.0% | |
| TTCACTTGTTCCTTCCATTT+AGG | + | tig0035033:880-899 | None:intergenic | 35.0% | |
| ACAAACACAGGATTATCTGA+AGG | - | tig0035033:907-926 | Msa1454090:CDS | 35.0% | |
| CAAACACAGGATTATCTGAA+GGG | - | tig0035033:908-927 | Msa1454090:CDS | 35.0% | |
| CTTTGGATGATGAATATAGC+TGG | - | tig0035033:932-951 | Msa1454090:exon | 35.0% | |
| CTGGAGAAAATATGGTCAAA+AGG | - | tig0035033:951-970 | Msa1454090:five_prime_UTR | 35.0% | |
| AATGAAGATGGGCACTTAAG+TGG | + | tig0035033:241-260 | None:intergenic | 40.0% | |
| ! | CATAGTAAGTGCTTTCTGGT+AGG | + | tig0035033:421-440 | None:intergenic | 40.0% |
| ACTATGTTGAATGTGAGAGG+AGG | - | tig0035033:435-454 | Msa1454090:intron | 40.0% | |
| GGACTCTCGTTTGTAAAAGA+AGG | + | tig0035033:501-520 | None:intergenic | 40.0% | |
| ! | GTCTATGATGACCTCACTTT+TGG | + | tig0035033:523-542 | None:intergenic | 40.0% |
| TGTGTGAATTGTCAAGGTCA+AGG | - | tig0035033:794-813 | Msa1454090:CDS | 40.0% | |
| ! | AAAGAGCATGCCTAAATGGA+AGG | - | tig0035033:867-886 | Msa1454090:CDS | 40.0% |
| GTGAAGATTTGCACAAACAC+AGG | - | tig0035033:895-914 | Msa1454090:CDS | 40.0% | |
| AGGATTATCTGAAGGGTCTT+TGG | - | tig0035033:915-934 | Msa1454090:CDS | 40.0% | |
| AATGAAGATGGGCACTTAAG+TGG | + | tig0035033:241-260 | None:intergenic | 40.0% | |
| ! | CATAGTAAGTGCTTTCTGGT+AGG | + | tig0035033:421-440 | None:intergenic | 40.0% |
| ACTATGTTGAATGTGAGAGG+AGG | - | tig0035033:435-454 | Msa1454090:intron | 40.0% | |
| GGACTCTCGTTTGTAAAAGA+AGG | + | tig0035033:501-520 | None:intergenic | 40.0% | |
| ! | GTCTATGATGACCTCACTTT+TGG | + | tig0035033:523-542 | None:intergenic | 40.0% |
| TGTGTGAATTGTCAAGGTCA+AGG | - | tig0035033:794-813 | Msa1454090:CDS | 40.0% | |
| ! | AAAGAGCATGCCTAAATGGA+AGG | - | tig0035033:867-886 | Msa1454090:CDS | 40.0% |
| GTGAAGATTTGCACAAACAC+AGG | - | tig0035033:895-914 | Msa1454090:CDS | 40.0% | |
| AGGATTATCTGAAGGGTCTT+TGG | - | tig0035033:915-934 | Msa1454090:CDS | 40.0% | |
| GCACTTAAGTGGAGTGATGA+AGG | + | tig0035033:230-249 | None:intergenic | 45.0% | |
| GCTTTGCTAACTCCTTTCCT+TGG | + | tig0035033:329-348 | None:intergenic | 45.0% | |
| GGAGAGACTAAGACCATTGA+TGG | - | tig0035033:456-475 | Msa1454090:intron | 45.0% | |
| AAACGAGAGTCCCAAAAGTG+AGG | - | tig0035033:509-528 | Msa1454090:intron | 45.0% | |
| AAGTGAGGTCATCATAGACC+AGG | - | tig0035033:524-543 | Msa1454090:intron | 45.0% | |
| ! | GAGCATTGTGGTTAAACTCC+TGG | + | tig0035033:545-564 | None:intergenic | 45.0% |
| ! | GCAGAAAGAGCATGCCTAAA+TGG | - | tig0035033:863-882 | Msa1454090:CDS | 45.0% |
| GCACTTAAGTGGAGTGATGA+AGG | + | tig0035033:230-249 | None:intergenic | 45.0% | |
| GCTTTGCTAACTCCTTTCCT+TGG | + | tig0035033:329-348 | None:intergenic | 45.0% | |
| GGAGAGACTAAGACCATTGA+TGG | - | tig0035033:456-475 | Msa1454090:intron | 45.0% | |
| AAACGAGAGTCCCAAAAGTG+AGG | - | tig0035033:509-528 | Msa1454090:intron | 45.0% | |
| AAGTGAGGTCATCATAGACC+AGG | - | tig0035033:524-543 | Msa1454090:intron | 45.0% | |
| ! | GAGCATTGTGGTTAAACTCC+TGG | + | tig0035033:545-564 | None:intergenic | 45.0% |
| ! | GCAGAAAGAGCATGCCTAAA+TGG | - | tig0035033:863-882 | Msa1454090:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0035033 | gene | 218 | 996 | 218 | ID=Msa1454090;Name=Msa1454090 |
| tig0035033 | mRNA | 218 | 996 | 218 | ID=Msa1454090-mRNA-1;Parent=Msa1454090;Name=Msa1454090-mRNA-1;_AED=0.00;_eAED=0.00;_QI=58|1|0.5|1|1|1|2|0|144 |
| tig0035033 | exon | 218 | 347 | 218 | ID=Msa1454090-mRNA-1:exon:19515;Parent=Msa1454090-mRNA-1 |
| tig0035033 | exon | 637 | 996 | 637 | ID=Msa1454090-mRNA-1:exon:19514;Parent=Msa1454090-mRNA-1 |
| tig0035033 | five_prime_UTR | 939 | 996 | 939 | ID=Msa1454090-mRNA-1:five_prime_utr;Parent=Msa1454090-mRNA-1 |
| tig0035033 | CDS | 637 | 938 | 637 | ID=Msa1454090-mRNA-1:cds;Parent=Msa1454090-mRNA-1 |
| tig0035033 | CDS | 218 | 347 | 218 | ID=Msa1454090-mRNA-1:cds;Parent=Msa1454090-mRNA-1 |
| Gene Sequence |
| Protein sequence |
