Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1466560 | KEH24332.1 | 94.561 | 239 | 12 | 1 | 1 | 239 | 1 | 238 | 4.29e-153 | 440 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1466560 | sp|Q7NJS7|PRMC_GLOVI | 41.667 | 144 | 74 | 3 | 101 | 238 | 22 | 161 | 5.89e-26 | 105 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1466560 | A0A072U3P1 | 94.561 | 239 | 12 | 1 | 1 | 239 | 1 | 238 | 2.05e-153 | 440 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0038350 | Msa1466560 | -0.806366 | 8.516714e-50 | -8.615850e-47 |
| Msa0789570 | Msa1466560 | 0.803504 | 3.378399e-49 | -8.615850e-47 |
| Msa1256540 | Msa1466560 | -0.803172 | 3.956678e-49 | -8.615850e-47 |
| Msa0442850 | Msa1466560 | 0.804797 | 1.818347e-49 | -8.615850e-47 |
| Msa0467900 | Msa1466560 | -0.811856 | 5.677420e-51 | -8.615850e-47 |
| Msa1008490 | Msa1466560 | 0.807010 | 6.228097e-50 | -8.615850e-47 |
| Msa1036390 | Msa1466560 | 0.802586 | 5.229200e-49 | -8.615850e-47 |
| Msa1040120 | Msa1466560 | 0.802085 | 6.630420e-49 | -8.615850e-47 |
| Msa1076680 | Msa1466560 | 0.801181 | 1.016324e-48 | -8.615850e-47 |
| Msa0342340 | Msa1466560 | -0.801123 | 1.044406e-48 | -8.615850e-47 |
| Msa1204980 | Msa1466560 | -0.803080 | 4.133811e-49 | -8.615850e-47 |
| Msa1205010 | Msa1466560 | -0.803271 | 3.775695e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1466560 | MtrunA17_Chr7g0268401 | 94.561 | 239 | 12 | 1 | 1 | 239 | 14 | 251 | 4.14e-157 | 440 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1466560 | AT5G64150.1 | 54.656 | 247 | 95 | 4 | 10 | 239 | 5 | 251 | 6.92e-88 | 265 |
Find 40 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGGGGTTTGGGCTGATTT+AGG | 0.146720 | tig0047545:+615 | Msa1466560:CDS |
| CCTGTAGCTGTTGCTGTTGC+AGG | 0.258741 | tig0047545:+717 | Msa1466560:CDS |
| GAGGGGTTTGGGCTGATTTA+GGG | 0.279292 | tig0047545:+616 | Msa1466560:CDS |
| GACTGAACTTATTGTTGATT+TGG | 0.301498 | tig0047545:+557 | Msa1466560:CDS |
| TGTTCAAAGGTATTCTTTGC+AGG | 0.342417 | tig0047545:+746 | Msa1466560:CDS |
| TTTGAGTTCCCTGCACAAAA+TGG | 0.347920 | tig0047545:-316 | Msa1466560:intergenic |
| AGGATTTGAAAAGAGGGGTT+TGG | 0.358688 | tig0047545:+604 | Msa1466560:CDS |
| GGATTTGAAAAGAGGGGTTT+GGG | 0.359508 | tig0047545:+605 | Msa1466560:CDS |
| AAGAATGAGGATTTGAAAAG+AGG | 0.374020 | tig0047545:+597 | Msa1466560:CDS |
| GGAAGTGGTGCTCTTGCTAT+TGG | 0.389038 | tig0047545:+642 | Msa1466560:CDS |
| TCTTGTTATCATCACCCATA+TGG | 0.399896 | tig0047545:-383 | Msa1466560:intergenic |
| TGAGAAGTTGTGATTTGTTA+AGG | 0.421647 | tig0047545:-61 | Msa1466560:intergenic |
| TAGAGGAACTATATTGTTTG+TGG | 0.431806 | tig0047545:+427 | Msa1466560:CDS |
| CTCTTGCTATTGGCATTGGT+AGG | 0.433770 | tig0047545:+652 | Msa1466560:CDS |
| TGATAGTGATGAAGAACAAA+AGG | 0.442696 | tig0047545:-121 | Msa1466560:intergenic |
| CATCCAACTATATATTGAAA+AGG | 0.469078 | tig0047545:-474 | Msa1466560:intergenic |
| AGAATGAGGATTTGAAAAGA+GGG | 0.470097 | tig0047545:+598 | Msa1466560:CDS |
| TGATGTTGTTTCAAAGAATG+AGG | 0.473095 | tig0047545:+584 | Msa1466560:CDS |
| ATGTGAGCATTGGAAGGATC+TGG | 0.474327 | tig0047545:+494 | Msa1466560:CDS |
| ATAAGTTCAGTCTCAGGTCT+AGG | 0.476338 | tig0047545:-546 | Msa1466560:intergenic |
| CTGCACAAAATGGTTGAGTC+TGG | 0.476906 | tig0047545:-306 | Msa1466560:intergenic |
| GAAAATGAAGGCAGATATAG+AGG | 0.483718 | tig0047545:+410 | Msa1466560:CDS |
| CCTGCAACAGCAACAGCTAC+AGG | 0.486713 | tig0047545:-717 | Msa1466560:intergenic |
| TCAACAATAAGTTCAGTCTC+AGG | 0.498019 | tig0047545:-552 | Msa1466560:intergenic |
| TATATTGTTTGTGGAAGCAA+AGG | 0.499336 | tig0047545:+436 | Msa1466560:CDS |
| GTTTGGGCTGATTTAGGGAC+TGG | 0.499341 | tig0047545:+621 | Msa1466560:CDS |
| TCTTGCTATTGGCATTGGTA+GGG | 0.505840 | tig0047545:+653 | Msa1466560:CDS |
| GGTGCTCTTGCTATTGGCAT+TGG | 0.506195 | tig0047545:+648 | Msa1466560:CDS |
| CTTGTTATCATCACCCATAT+GGG | 0.507528 | tig0047545:-382 | Msa1466560:intergenic |
| GCTGATTTAGGGACTGGAAG+TGG | 0.510435 | tig0047545:+627 | Msa1466560:CDS |
| GAATGAGGATTTGAAAAGAG+GGG | 0.534849 | tig0047545:+599 | Msa1466560:CDS |
| TAACAAGAGGGTGAAAATGA+AGG | 0.546621 | tig0047545:+398 | Msa1466560:CDS |
| ATATAGTTGGATGTGAGCAT+TGG | 0.548726 | tig0047545:+484 | Msa1466560:CDS |
| GGAAGCAAAGGATTGAAGAA+AGG | 0.552507 | tig0047545:+448 | Msa1466560:CDS |
| TTGCAGGTTACAATGTTCAA+AGG | 0.574872 | tig0047545:+733 | Msa1466560:CDS |
| GATAGTGATGAAGAACAAAA+GGG | 0.575128 | tig0047545:-120 | Msa1466560:intergenic |
| AGTTGGATGTGAGCATTGGA+AGG | 0.610910 | tig0047545:+488 | Msa1466560:CDS |
| GGAACTCAAATGGTTCATTG+AGG | 0.660332 | tig0047545:+329 | Msa1466560:CDS |
| ATATGGGTGATGATAACAAG+AGG | 0.660513 | tig0047545:+385 | Msa1466560:CDS |
| TATGGGTGATGATAACAAGA+GGG | 0.709247 | tig0047545:+386 | Msa1466560:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAACATTGCTTTTTCAAT+TGG | + | tig0047545:255-274 | Msa1466560:CDS | 20.0% |
| !!! | AAAACATTGCTTTTTCAATT+GGG | + | tig0047545:256-275 | Msa1466560:CDS | 20.0% |
| !!! | AAACCTTTTCAATATATAGT+TGG | + | tig0047545:471-490 | Msa1466560:CDS | 20.0% |
| !! | CTCAATTTTGTTGAGTAAAT+GGG | - | tig0047545:207-226 | None:intergenic | 25.0% |
| !! | ACTCAATTTTGTTGAGTAAA+TGG | - | tig0047545:208-227 | None:intergenic | 25.0% |
| !! | TCTTTTGTTCAATCAGATAA+TGG | + | tig0047545:282-301 | Msa1466560:CDS | 25.0% |
| ! | CATCCAACTATATATTGAAA+AGG | - | tig0047545:477-496 | Msa1466560:intergenic | 25.0% |
| !! | TGAGAAGTTGTGATTTGTTA+AGG | - | tig0047545:64-83 | None:intergenic | 30.0% |
| GATAGTGATGAAGAACAAAA+GGG | - | tig0047545:123-142 | None:intergenic | 30.0% | |
| ! | TGATAGTGATGAAGAACAAA+AGG | - | tig0047545:124-143 | None:intergenic | 30.0% |
| TCTCAAAAAGATTGGAACTT+GGG | - | tig0047545:181-200 | None:intergenic | 30.0% | |
| ! | AATTTTGTTGAGTAAATGGG+CGG | - | tig0047545:204-223 | None:intergenic | 30.0% |
| AATTGAGTGACCTCAAAAAA+TGG | + | tig0047545:220-239 | Msa1466560:CDS | 30.0% | |
| ACCTCAAAAAATGGCATAAT+TGG | + | tig0047545:229-248 | Msa1466560:CDS | 30.0% | |
| CCTCAAAAAATGGCATAATT+GGG | + | tig0047545:230-249 | Msa1466560:CDS | 30.0% | |
| TAGAGGAACTATATTGTTTG+TGG | + | tig0047545:427-446 | Msa1466560:CDS | 30.0% | |
| TATATTGTTTGTGGAAGCAA+AGG | + | tig0047545:436-455 | Msa1466560:CDS | 30.0% | |
| ! | GACTGAACTTATTGTTGATT+TGG | + | tig0047545:557-576 | Msa1466560:CDS | 30.0% |
| !! | TGATGTTGTTTCAAAGAATG+AGG | + | tig0047545:584-603 | Msa1466560:CDS | 30.0% |
| AAGAATGAGGATTTGAAAAG+AGG | + | tig0047545:597-616 | Msa1466560:CDS | 30.0% | |
| AGAATGAGGATTTGAAAAGA+GGG | + | tig0047545:598-617 | Msa1466560:CDS | 30.0% | |
| CTCAAAAAGATTGGAACTTG+GGG | - | tig0047545:180-199 | None:intergenic | 35.0% | |
| GTCTCAAAAAGATTGGAACT+TGG | - | tig0047545:182-201 | None:intergenic | 35.0% | |
| !!! | CCCAATTATGCCATTTTTTG+AGG | - | tig0047545:233-252 | Msa1466560:intergenic | 35.0% |
| !!! | CACTCACTTTTTTCCCATAT+GGG | + | tig0047545:369-388 | Msa1466560:CDS | 35.0% |
| ! | CTTGTTATCATCACCCATAT+GGG | - | tig0047545:385-404 | Msa1466560:intergenic | 35.0% |
| ! | TCTTGTTATCATCACCCATA+TGG | - | tig0047545:386-405 | Msa1466560:intergenic | 35.0% |
| ATATGGGTGATGATAACAAG+AGG | + | tig0047545:385-404 | Msa1466560:CDS | 35.0% | |
| TATGGGTGATGATAACAAGA+GGG | + | tig0047545:386-405 | Msa1466560:CDS | 35.0% | |
| TAACAAGAGGGTGAAAATGA+AGG | + | tig0047545:398-417 | Msa1466560:CDS | 35.0% | |
| GAAAATGAAGGCAGATATAG+AGG | + | tig0047545:410-429 | Msa1466560:CDS | 35.0% | |
| ATATAGTTGGATGTGAGCAT+TGG | + | tig0047545:484-503 | Msa1466560:CDS | 35.0% | |
| !!! | TGGTTTTGAGTGTTCAAGAA+GGG | + | tig0047545:514-533 | Msa1466560:CDS | 35.0% |
| TCAACAATAAGTTCAGTCTC+AGG | - | tig0047545:555-574 | Msa1466560:intergenic | 35.0% | |
| GAATGAGGATTTGAAAAGAG+GGG | + | tig0047545:599-618 | Msa1466560:CDS | 35.0% | |
| TTGCAGGTTACAATGTTCAA+AGG | + | tig0047545:733-752 | Msa1466560:CDS | 35.0% | |
| !! | ACTTGGGGTTTTGTAGAAGT+GGG | - | tig0047545:165-184 | None:intergenic | 40.0% |
| !! | AACTTGGGGTTTTGTAGAAG+TGG | - | tig0047545:166-185 | None:intergenic | 40.0% |
| ! | AGACTCAACCATTTTGTGCA+GGG | + | tig0047545:308-327 | Msa1466560:CDS | 40.0% |
| ! | TTTGAGTTCCCTGCACAAAA+TGG | - | tig0047545:319-338 | Msa1466560:intergenic | 40.0% |
| ! | TTTTGTGCAGGGAACTCAAA+TGG | + | tig0047545:319-338 | Msa1466560:CDS | 40.0% |
| GGAACTCAAATGGTTCATTG+AGG | + | tig0047545:329-348 | Msa1466560:CDS | 40.0% | |
| CCATATGGGAAAAAAGTGAG+TGG | - | tig0047545:371-390 | Msa1466560:intergenic | 40.0% | |
| !!! | CCACTCACTTTTTTCCCATA+TGG | + | tig0047545:368-387 | Msa1466560:CDS | 40.0% |
| GGAAGCAAAGGATTGAAGAA+AGG | + | tig0047545:448-467 | Msa1466560:CDS | 40.0% | |
| !!! | CTGGTTTTGAGTGTTCAAGA+AGG | + | tig0047545:513-532 | Msa1466560:CDS | 40.0% |
| !!! | GGTTTTGAGTGTTCAAGAAG+GGG | + | tig0047545:515-534 | Msa1466560:CDS | 40.0% |
| ATAAGTTCAGTCTCAGGTCT+AGG | - | tig0047545:549-568 | Msa1466560:intergenic | 40.0% | |
| AGGATTTGAAAAGAGGGGTT+TGG | + | tig0047545:604-623 | Msa1466560:CDS | 40.0% | |
| GGATTTGAAAAGAGGGGTTT+GGG | + | tig0047545:605-624 | Msa1466560:CDS | 40.0% | |
| !! | TCTTGCTATTGGCATTGGTA+GGG | + | tig0047545:653-672 | Msa1466560:CDS | 40.0% |
| !!! | ATTGGCATTGGTAGGGTTTT+AGG | + | tig0047545:660-679 | Msa1466560:CDS | 40.0% |
| !!! | ATTGGTAGGGTTTTAGGAGA+TGG | + | tig0047545:666-685 | Msa1466560:CDS | 40.0% |
| ATGGGCGGTCTCAAAAAGAT+TGG | - | tig0047545:189-208 | None:intergenic | 45.0% | |
| CTGCACAAAATGGTTGAGTC+TGG | - | tig0047545:309-328 | Msa1466560:intergenic | 45.0% | |
| ! | CAGACTCAACCATTTTGTGC+AGG | + | tig0047545:307-326 | Msa1466560:CDS | 45.0% |
| !! | AGTTGGATGTGAGCATTGGA+AGG | + | tig0047545:488-507 | Msa1466560:CDS | 45.0% |
| !! | ATGTGAGCATTGGAAGGATC+TGG | + | tig0047545:494-513 | Msa1466560:CDS | 45.0% |
| !! | CTCTTGCTATTGGCATTGGT+AGG | + | tig0047545:652-671 | Msa1466560:CDS | 45.0% |
| !!! | GGTTTTAGGAGATGGTGGAA+AGG | + | tig0047545:674-693 | Msa1466560:CDS | 45.0% |
| ! | AGAGGGGTTTGGGCTGATTT+AGG | + | tig0047545:615-634 | Msa1466560:CDS | 50.0% |
| ! | GAGGGGTTTGGGCTGATTTA+GGG | + | tig0047545:616-635 | Msa1466560:CDS | 50.0% |
| ! | GTTTGGGCTGATTTAGGGAC+TGG | + | tig0047545:621-640 | Msa1466560:CDS | 50.0% |
| ! | GCTGATTTAGGGACTGGAAG+TGG | + | tig0047545:627-646 | Msa1466560:CDS | 50.0% |
| ! | GGAAGTGGTGCTCTTGCTAT+TGG | + | tig0047545:642-661 | Msa1466560:CDS | 50.0% |
| !! | GGTGCTCTTGCTATTGGCAT+TGG | + | tig0047545:648-667 | Msa1466560:CDS | 50.0% |
| !!! | GGTAGGGTTTTAGGAGATGG+TGG | + | tig0047545:669-688 | Msa1466560:CDS | 50.0% |
| CCTGCAACAGCAACAGCTAC+AGG | - | tig0047545:720-739 | Msa1466560:intergenic | 55.0% | |
| ! | CCTGTAGCTGTTGCTGTTGC+AGG | + | tig0047545:717-736 | Msa1466560:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0047545 | gene | 51 | 767 | 51 | ID=Msa1466560;Name=Msa1466560 |
| tig0047545 | mRNA | 51 | 767 | 51 | ID=Msa1466560-mRNA-1;Parent=Msa1466560;Name=Msa1466560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|239 |
| tig0047545 | exon | 51 | 767 | 51 | ID=Msa1466560-mRNA-1:exon:25094;Parent=Msa1466560-mRNA-1 |
| tig0047545 | CDS | 51 | 767 | 51 | ID=Msa1466560-mRNA-1:cds;Parent=Msa1466560-mRNA-1 |
| Gene Sequence |
| Protein sequence |
