AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000539

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000539.t1 MTR_8g096620 97.721 351 8 0 1 351 1 351 0.0 702
MS.gene000539.t1 MTR_8g096580 48.077 312 148 2 1 311 1 299 3.40e-102 304
MS.gene000539.t1 MTR_5g008850 44.937 316 163 5 1 310 1 311 2.71e-85 263
MS.gene000539.t1 MTR_8g096610 35.751 193 90 7 126 311 54 219 3.70e-27 107
MS.gene000539.t1 MTR_7g080410 35.821 201 120 6 43 236 36 234 3.23e-24 101
MS.gene000539.t1 MTR_7g080410 35.821 201 120 6 43 236 36 234 4.10e-24 100
MS.gene000539.t1 MTR_3g087850 29.528 254 152 10 49 289 30 269 2.16e-19 87.4
MS.gene000539.t1 MTR_7g105590 29.101 189 122 5 47 229 10 192 5.97e-18 81.3
MS.gene000539.t1 MTR_8g442750 31.390 223 137 6 40 250 9 227 1.30e-17 82.4
MS.gene000539.t1 MTR_3g085740 27.232 224 147 7 43 263 73 283 4.44e-17 81.3
MS.gene000539.t1 MTR_3g087860 28.390 236 149 9 63 289 30 254 7.06e-17 80.1
MS.gene000539.t1 MTR_4g132850 27.111 225 146 4 41 252 3 222 9.73e-17 79.3
MS.gene000539.t1 MTR_2g022600 30.180 222 132 7 45 250 33 247 2.10e-16 79.7
MS.gene000539.t1 MTR_1g082760 27.778 198 134 4 40 229 34 230 2.40e-16 79.0
MS.gene000539.t1 MTR_7g113650 26.400 250 171 6 40 277 34 282 7.04e-16 77.4
MS.gene000539.t1 MTR_4g058840 28.358 201 135 6 43 235 9 208 8.44e-15 73.9
MS.gene000539.t1 MTR_4g058820 28.358 201 135 6 43 235 9 208 8.44e-15 73.9
MS.gene000539.t1 MTR_6g023610 28.426 197 129 6 45 234 13 204 9.54e-15 73.9
MS.gene000539.t1 MTR_7g063390 29.310 232 144 9 44 267 15 234 1.40e-14 73.2
MS.gene000539.t1 MTR_4g052340 26.267 217 140 6 47 253 16 222 1.49e-14 73.2
MS.gene000539.t1 MTR_4g058810 27.861 201 136 6 43 235 9 208 3.22e-14 72.4
MS.gene000539.t1 MTR_4g086410 27.500 200 133 4 46 233 5 204 3.27e-14 73.9
MS.gene000539.t1 MTR_4g052300 29.319 191 113 5 47 226 10 189 4.90e-14 71.6
MS.gene000539.t1 MTR_6g023590 28.426 197 129 6 45 234 13 204 5.87e-14 71.6
MS.gene000539.t1 MTR_4g052320 28.500 200 121 5 47 235 10 198 7.69e-14 71.2
MS.gene000539.t1 MTR_4g052350 26.512 215 142 7 47 253 16 222 8.89e-14 70.9
MS.gene000539.t1 MTR_1g051165 27.000 200 134 5 48 235 17 216 9.94e-14 70.9
MS.gene000539.t1 MTR_1196s0010 31.579 209 131 5 33 236 262 463 1.04e-13 72.4
MS.gene000539.t1 MTR_4g086400 26.500 200 135 4 46 233 5 204 2.04e-13 71.6
MS.gene000539.t1 MTR_4g097510 26.804 194 134 6 48 238 5 193 7.52e-13 68.6
MS.gene000539.t1 MTR_7g063360 29.075 227 151 7 44 267 15 234 1.94e-12 67.0
MS.gene000539.t1 MTR_7g076660 32.414 145 86 3 52 184 12 156 2.73e-12 67.0
MS.gene000539.t1 MTR_7g076660 32.414 145 86 3 52 184 11 155 3.08e-12 66.6
MS.gene000539.t1 MTR_7g063270 28.571 224 149 7 47 267 18 233 3.48e-12 66.2
MS.gene000539.t1 MTR_7g071680 27.368 190 129 4 42 226 26 211 6.98e-12 65.9
MS.gene000539.t1 MTR_4g131740 26.425 193 129 6 47 229 42 231 9.77e-12 65.5
MS.gene000539.t1 MTR_3g098750 30.415 217 115 9 50 250 159 355 1.09e-11 66.2
MS.gene000539.t1 MTR_7g063260 26.906 223 144 6 47 252 18 238 1.43e-11 64.3
MS.gene000539.t1 MTR_4g078630 27.230 213 137 6 42 243 16 221 1.61e-11 64.7
MS.gene000539.t1 MTR_7g076655 31.724 145 87 3 52 184 11 155 1.77e-11 64.3
MS.gene000539.t1 MTR_7g094520 26.457 223 149 6 48 255 14 236 2.82e-11 63.5
MS.gene000539.t1 MTR_4g064887 25.778 225 151 6 47 267 17 229 3.39e-11 63.5
MS.gene000539.t1 MTR_7g094510 28.400 250 148 10 48 277 9 247 8.99e-11 62.0
MS.gene000539.t1 MTR_3g068070 26.339 224 151 6 47 267 17 229 9.47e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000539.t1 AT5G50600 61.254 351 134 2 1 351 1 349 1.06e-152 433
MS.gene000539.t1 AT5G50700 61.254 351 134 2 1 351 1 349 1.06e-152 433
MS.gene000539.t1 AT5G50770 50.147 339 167 2 1 339 1 337 1.57e-112 331
MS.gene000539.t1 AT3G47350 47.284 313 151 3 1 313 1 299 1.28e-101 302
MS.gene000539.t1 AT5G50690 49.505 303 142 2 1 303 1 292 1.65e-98 294
MS.gene000539.t1 AT5G50590 49.505 303 142 2 1 303 1 292 1.65e-98 294
MS.gene000539.t1 AT3G47360 46.984 315 150 3 1 313 1 300 9.55e-96 287
MS.gene000539.t1 AT4G10020 45.912 318 165 4 1 316 2 314 4.75e-92 280
MS.gene000539.t1 AT3G47350 49.823 283 126 4 1 283 1 267 6.74e-91 275
MS.gene000539.t1 AT5G50690 53.975 239 110 0 1 239 1 239 1.76e-84 256
MS.gene000539.t1 AT5G50590 53.975 239 110 0 1 239 1 239 1.76e-84 256
MS.gene000539.t1 AT3G47350 39.297 313 132 4 1 313 1 255 1.59e-72 226
MS.gene000539.t1 AT3G03330 35.088 228 135 7 17 231 6 233 6.99e-25 103
MS.gene000539.t1 AT5G65205 34.211 190 111 7 49 233 10 190 1.49e-22 96.3
MS.gene000539.t1 AT5G10050 31.606 193 122 5 49 238 9 194 2.33e-22 95.5
MS.gene000539.t1 AT1G24360 29.592 196 131 6 43 234 72 264 2.03e-17 82.4
MS.gene000539.t1 AT1G63380 28.934 197 132 5 48 236 25 221 2.26e-17 81.6
MS.gene000539.t1 AT1G54870 29.648 199 129 6 40 229 76 272 2.27e-17 82.4
MS.gene000539.t1 AT1G54870 29.648 199 129 6 40 229 78 274 2.57e-17 82.4
MS.gene000539.t1 AT3G05260 29.648 199 126 7 39 227 30 224 3.11e-17 81.3
MS.gene000539.t1 AT3G12800 30.594 219 129 6 40 243 5 215 3.35e-17 81.3
MS.gene000539.t1 AT1G63380 28.934 197 132 5 48 236 18 214 3.52e-17 80.9
MS.gene000539.t1 AT1G63380 28.934 197 132 5 48 236 22 218 4.34e-17 80.9
MS.gene000539.t1 AT1G63380 28.934 197 132 5 48 236 20 216 4.65e-17 80.9
MS.gene000539.t1 AT1G62610 28.934 197 132 5 48 236 17 213 4.32e-16 77.8
MS.gene000539.t1 AT1G62610 28.934 197 132 5 48 236 16 212 4.43e-16 77.8
MS.gene000539.t1 AT1G62610 28.934 197 132 5 48 236 18 214 4.46e-16 77.8
MS.gene000539.t1 AT1G62610 28.934 197 132 5 48 236 22 218 4.61e-16 77.8
MS.gene000539.t1 AT2G29350 31.466 232 134 9 44 267 14 228 3.22e-15 75.1
MS.gene000539.t1 AT3G55310 28.000 200 127 6 48 235 20 214 7.92e-15 74.3
MS.gene000539.t1 AT2G17845 27.500 200 133 6 48 236 50 248 2.78e-14 73.2
MS.gene000539.t1 AT4G05530 26.154 195 130 5 41 229 5 191 2.81e-14 72.4
MS.gene000539.t1 AT3G55290 28.061 196 134 5 48 236 20 215 7.38e-14 71.6
MS.gene000539.t1 AT3G55290 28.061 196 134 5 48 236 21 216 7.53e-14 71.2
MS.gene000539.t1 AT5G06060 27.948 229 147 8 44 267 8 223 1.53e-13 70.5
MS.gene000539.t1 AT3G46170 27.041 196 136 5 48 236 29 224 3.48e-13 69.7
MS.gene000539.t1 AT1G67730 26.296 270 169 14 16 261 12 275 1.04e-12 68.6
MS.gene000539.t1 AT2G29290 30.493 223 133 9 52 267 14 221 1.18e-12 67.8
MS.gene000539.t1 AT4G13180 25.764 229 149 6 45 255 13 238 1.24e-12 67.8
MS.gene000539.t1 AT3G06060 31.034 203 114 7 52 238 44 236 1.74e-12 67.8
MS.gene000539.t1 AT3G06060 31.034 203 114 7 52 238 61 253 1.78e-12 68.2
MS.gene000539.t1 AT5G18210 26.180 233 148 7 44 255 7 236 2.32e-12 66.6
MS.gene000539.t1 AT5G18210 26.180 233 148 7 44 255 7 236 2.70e-12 67.0
MS.gene000539.t1 AT4G24050 29.302 215 118 8 52 237 39 248 1.45e-11 64.7
MS.gene000539.t1 AT2G29370 28.384 229 146 7 44 267 15 230 1.47e-11 64.7
MS.gene000539.t1 AT5G19200 29.949 197 121 6 50 235 40 230 1.93e-11 64.7
MS.gene000539.t1 AT4G24050 29.302 215 118 8 52 237 39 248 2.13e-11 64.7
MS.gene000539.t1 AT3G26760 25.980 204 143 5 35 234 26 225 3.62e-11 63.9
MS.gene000539.t1 AT3G03980 26.829 205 137 5 42 233 11 215 3.65e-11 63.5
MS.gene000539.t1 AT1G52340 28.788 198 123 6 47 233 20 210 9.37e-11 62.4

Find 78 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTAGGTGCTTCTCTTGC+TGG 0.240546 4.3:-6715132 None:intergenic
TACATTATATATACTCCTTC+TGG 0.243370 4.3:-6714518 None:intergenic
TCCTTTCATCAATTGATTCT+TGG 0.265350 4.3:+6714485 MS.gene000539:CDS
GATGAAACCGTTAACCATTT+TGG 0.281093 4.3:+6714108 MS.gene000539:CDS
GCTGATAGAGCTCGTGATTT+TGG 0.286452 4.3:+6714021 MS.gene000539:CDS
AAACCCCACTCCAACAATTC+TGG 0.298326 4.3:-6715075 None:intergenic
TTTGGTAGGACACAAACTTT+TGG 0.338368 4.3:+6714398 MS.gene000539:intron
AGGGAAGAATGTAAAGATAA+TGG 0.356930 4.3:-6713784 None:intergenic
CATACATACGTCTACCAAAA+TGG 0.358019 4.3:-6714122 None:intergenic
AAGAATTTGAAGGTCCAATA+TGG 0.365021 4.3:-6713756 None:intergenic
TTTCCAAAGTTGATGATTGT+AGG 0.374144 4.3:+6714076 MS.gene000539:CDS
ATTGACACCAGAAGGCAAAA+TGG 0.374515 4.3:+6714790 MS.gene000539:CDS
TTGTTACATCTCTCATATCT+TGG 0.383129 4.3:-6714820 None:intergenic
TTCTTCCCTGAAAATGTTGC+TGG 0.384509 4.3:+6713798 MS.gene000539:CDS
ATGCATTACAGTGGATCATT+TGG 0.398219 4.3:+6714219 MS.gene000539:intron
GGTTCAGGAACTTATGAATA+AGG 0.407519 4.3:-6713686 None:intergenic
AGTTGATGATTGTAGGCGTT+TGG 0.425267 4.3:+6714083 MS.gene000539:CDS
CACATCACCGGTGCTTCCTC+TGG 0.427479 4.3:+6713831 MS.gene000539:CDS
TGTCAATAAGACTTTGCCTT+TGG 0.427890 4.3:-6714774 None:intergenic
ACCGGTGCTTCCTCTGGTAT+TGG 0.428806 4.3:+6713837 MS.gene000539:CDS
ACGTCTACCAAAATGGTTAA+CGG 0.443115 4.3:-6714115 None:intergenic
TCCAATATGGTGGTAAGAAA+AGG 0.443917 4.3:-6713743 None:intergenic
GAAAAGGAGTAAGGAAAAGA+AGG 0.450873 4.3:-6713727 None:intergenic
AGTAAGGAAAAGAAGGTGAA+TGG 0.457421 4.3:-6713720 None:intergenic
ACGCCTACAATCATCAACTT+TGG 0.479547 4.3:-6714079 None:intergenic
ATGGAGGAGCTACTAGGTTC+AGG 0.479762 4.3:-6713701 None:intergenic
ACATTATATATACTCCTTCT+GGG 0.481364 4.3:-6714517 None:intergenic
TGAGGTGTGGTAAAGCAAAA+CGG 0.485877 4.3:-6714438 None:intergenic
GTGTCCAGAATTGTTGGAGT+GGG 0.486451 4.3:+6715071 MS.gene000539:CDS
TTGACACCAGAAGGCAAAAT+GGG 0.489713 4.3:+6714791 MS.gene000539:CDS
TATATACTCCTTCTGGGTGC+AGG 0.491452 4.3:-6714511 None:intergenic
AAGTCTTTATGAAACATTGA+GGG 0.498519 4.3:+6714688 MS.gene000539:CDS
TGGTGGTAAGAAAAGGAGTA+AGG 0.505040 4.3:-6713736 None:intergenic
CCTCTGCTGTTTCTGAGGTG+TGG 0.510515 4.3:-6714451 None:intergenic
GCACGCATTATAATAACATC+AGG 0.515853 4.3:-6714048 None:intergenic
TAGCTTTAAATTACCATGAG+AGG 0.516356 4.3:-6714307 None:intergenic
GAAACATTGAGGGTTGAAGT+TGG 0.516747 4.3:+6714698 MS.gene000539:CDS
ATGGTACATTGAGGGGAGAT+AGG 0.520717 4.3:+6714998 MS.gene000539:CDS
GAGGTGTGGTAAAGCAAAAC+GGG 0.520958 4.3:-6714437 None:intergenic
TACCATGAGAGGTCTCCAAT+TGG 0.521548 4.3:-6714296 None:intergenic
AGTGTTGTGTCCAGAATTGT+TGG 0.521927 4.3:+6715065 MS.gene000539:CDS
AGGTGAATGGAGGAGCTACT+AGG 0.526268 4.3:-6713707 None:intergenic
AATGGAAAGGAAGAATTTGA+AGG 0.527999 4.3:-6713766 None:intergenic
AATAGTGAATGGTACATTGA+GGG 0.532567 4.3:+6714990 MS.gene000539:CDS
TCAGGAGATCGAATTGAAGA+TGG 0.534824 4.3:-6715195 None:intergenic
ACTCATTCAGTCTTGACCTC+AGG 0.535309 4.3:-6715213 None:intergenic
AATTCAGATTCTATGTAACC+TGG 0.554617 4.3:-6714749 None:intergenic
ATGTAACTGATATCACCAAT+TGG 0.561569 4.3:+6714281 MS.gene000539:CDS
TAAGTCTTTATGAAACATTG+AGG 0.565380 4.3:+6714687 MS.gene000539:CDS
CACCAATTGGAGACCTCTCA+TGG 0.567087 4.3:+6714294 MS.gene000539:CDS
TCCAAGAATCAATTGATGAA+AGG 0.573578 4.3:-6714486 None:intergenic
GGTAAAGTTGTTCACATCAC+CGG 0.574641 4.3:+6713819 MS.gene000539:CDS
CAATAGTGAATGGTACATTG+AGG 0.574939 4.3:+6714989 MS.gene000539:CDS
CATTCACTCACTGATCCCAC+TGG 0.576814 4.3:-6714958 None:intergenic
AGTTACATCTTCAAACATCA+TGG 0.578048 4.3:-6714266 None:intergenic
ACCAGAAGGCAAAATGGGCG+TGG 0.578451 4.3:+6714796 MS.gene000539:CDS
TCCAATACCAGAGGAAGCAC+CGG 0.584460 4.3:-6713838 None:intergenic
CTTGGATGCCTGCACCCAGA+AGG 0.586038 4.3:+6714503 MS.gene000539:CDS
TATGTCATTCTAAACCATGC+TGG 0.590361 4.3:-6715033 None:intergenic
TGTGCAAAATCAATAGTGAA+TGG 0.591847 4.3:+6714979 MS.gene000539:CDS
TGAAGTGAGTGCAACTCCAG+TGG 0.598795 4.3:+6714942 MS.gene000539:CDS
GGTATTTGACTGCACCAGCA+TGG 0.600958 4.3:+6715019 MS.gene000539:CDS
AAAGTCTTATTGACACCAGA+AGG 0.602673 4.3:+6714782 MS.gene000539:CDS
TGTCCAGAATTGTTGGAGTG+GGG 0.604050 4.3:+6715072 MS.gene000539:CDS
AAGGAAAAGAAGGTGAATGG+AGG 0.605287 4.3:-6713717 None:intergenic
TGCTTCCTCTGGTATTGGAG+AGG 0.611269 4.3:+6713842 MS.gene000539:CDS
GAAGTGAGTGCAACTCCAGT+GGG 0.616438 4.3:+6714943 MS.gene000539:CDS
ATCTTCAATTCGATCTCCTG+AGG 0.626026 4.3:+6715197 MS.gene000539:CDS
TGTGTCCAGAATTGTTGGAG+TGG 0.626150 4.3:+6715070 MS.gene000539:CDS
AAACAGCAGAGGCAAAATTG+TGG 0.631748 4.3:+6714462 MS.gene000539:CDS
ATAGAATCTGAATTAACCAA+AGG 0.631775 4.3:+6714758 MS.gene000539:CDS
ATAGTGAATGGTACATTGAG+GGG 0.634116 4.3:+6714991 MS.gene000539:CDS
GGTGTAACAATTGTTACACC+AGG 0.641548 4.3:+6714731 MS.gene000539:CDS
AGAATGTAAAGATAATGGAA+AGG 0.645582 4.3:-6713779 None:intergenic
GCTTACGAGTATGCTAAGAG+AGG 0.664397 4.3:+6713949 MS.gene000539:CDS
AATTTGAAGGTCCAATATGG+TGG 0.665878 4.3:-6713753 None:intergenic
GTTGAAGTTGGAGCAGACGT+AGG 0.674435 4.3:+6714710 MS.gene000539:CDS
CCACACCTCAGAAACAGCAG+AGG 0.715008 4.3:+6714451 MS.gene000539:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTAATTAATATTTGTTTA+AGG - chr4.3:6714163-6714182 None:intergenic 10.0%
!!! ATTTTAATTTTTGTAGCATT+TGG + chr4.3:6713927-6713946 MS.gene000539:intron 15.0%
!! TGTTATGATGATATATATGT+AGG + chr4.3:6714918-6714937 MS.gene000539:intron 20.0%
!!! TATTTATTTGCTGTGTATTT+TGG + chr4.3:6714380-6714399 MS.gene000539:intron 20.0%
!!! TTTAATTTACCTAGTAATAC+AGG + chr4.3:6714860-6714879 MS.gene000539:intron 20.0%
! AAGTCTTTATGAAACATTGA+GGG + chr4.3:6714688-6714707 MS.gene000539:CDS 25.0%
! ACATTATATATACTCCTTCT+GGG - chr4.3:6714520-6714539 None:intergenic 25.0%
! ATAGAATCTGAATTAACCAA+AGG + chr4.3:6714758-6714777 MS.gene000539:CDS 25.0%
! TAAGTCTTTATGAAACATTG+AGG + chr4.3:6714687-6714706 MS.gene000539:CDS 25.0%
! TACATTATATATACTCCTTC+TGG - chr4.3:6714521-6714540 None:intergenic 25.0%
!!! AGAATGTAAAGATAATGGAA+AGG - chr4.3:6713782-6713801 None:intergenic 25.0%
AAGAATTTGAAGGTCCAATA+TGG - chr4.3:6713759-6713778 None:intergenic 30.0%
AATAGTGAATGGTACATTGA+GGG + chr4.3:6714990-6715009 MS.gene000539:CDS 30.0%
AATGGAAAGGAAGAATTTGA+AGG - chr4.3:6713769-6713788 None:intergenic 30.0%
AATTCAGATTCTATGTAACC+TGG - chr4.3:6714752-6714771 None:intergenic 30.0%
AGGGAAGAATGTAAAGATAA+TGG - chr4.3:6713787-6713806 None:intergenic 30.0%
AGTTACATCTTCAAACATCA+TGG - chr4.3:6714269-6714288 None:intergenic 30.0%
ATGTAACTGATATCACCAAT+TGG + chr4.3:6714281-6714300 MS.gene000539:CDS 30.0%
ATTCCCAATTCAACTCAATA+AGG - chr4.3:6714352-6714371 None:intergenic 30.0%
TAGTTATAGCCTGTATTACT+AGG - chr4.3:6714872-6714891 None:intergenic 30.0%
TCCAAGAATCAATTGATGAA+AGG - chr4.3:6714489-6714508 None:intergenic 30.0%
TGTGCAAAATCAATAGTGAA+TGG + chr4.3:6714979-6714998 MS.gene000539:CDS 30.0%
TTGTTACATCTCTCATATCT+TGG - chr4.3:6714823-6714842 None:intergenic 30.0%
! AAAAAGATTTTGGATGCTAC+TGG + chr4.3:6715153-6715172 MS.gene000539:CDS 30.0%
! TCCTTTCATCAATTGATTCT+TGG + chr4.3:6714485-6714504 MS.gene000539:CDS 30.0%
!! TAGCTTTAAATTACCATGAG+AGG - chr4.3:6714310-6714329 None:intergenic 30.0%
!! TATTGAGTTGAATTGGGAAT+TGG + chr4.3:6714352-6714371 MS.gene000539:intron 30.0%
!! TTTCCAAAGTTGATGATTGT+AGG + chr4.3:6714076-6714095 MS.gene000539:CDS 30.0%
!!! TATTTGCTGTGTATTTTGGT+AGG + chr4.3:6714384-6714403 MS.gene000539:intron 30.0%
AATTTGAAGGTCCAATATGG+TGG - chr4.3:6713756-6713775 None:intergenic 35.0%
ACGTCTACCAAAATGGTTAA+CGG - chr4.3:6714118-6714137 None:intergenic 35.0%
AGTAAGGAAAAGAAGGTGAA+TGG - chr4.3:6713723-6713742 None:intergenic 35.0%
ATAGTGAATGGTACATTGAG+GGG + chr4.3:6714991-6715010 MS.gene000539:CDS 35.0%
ATGCATTACAGTGGATCATT+TGG + chr4.3:6714219-6714238 MS.gene000539:intron 35.0%
CAATAGTGAATGGTACATTG+AGG + chr4.3:6714989-6715008 MS.gene000539:CDS 35.0%
CATACATACGTCTACCAAAA+TGG - chr4.3:6714125-6714144 None:intergenic 35.0%
GAAAAGGAGTAAGGAAAAGA+AGG - chr4.3:6713730-6713749 None:intergenic 35.0%
GCACGCATTATAATAACATC+AGG - chr4.3:6714051-6714070 None:intergenic 35.0%
GCTAACTACATGACTTTATC+TGG + chr4.3:6714326-6714345 MS.gene000539:intron 35.0%
GGTTCAGGAACTTATGAATA+AGG - chr4.3:6713689-6713708 None:intergenic 35.0%
TATGTCATTCTAAACCATGC+TGG - chr4.3:6715036-6715055 None:intergenic 35.0%
TCCAATATGGTGGTAAGAAA+AGG - chr4.3:6713746-6713765 None:intergenic 35.0%
TGTCAATAAGACTTTGCCTT+TGG - chr4.3:6714777-6714796 None:intergenic 35.0%
TTCTCTGTAATGCATTACAG+TGG + chr4.3:6714210-6714229 MS.gene000539:intron 35.0%
! ACTTTACCAGCAACATTTTC+AGG - chr4.3:6713807-6713826 None:intergenic 35.0%
! AGCACCTAGCAAAAAGATTT+TGG + chr4.3:6715143-6715162 MS.gene000539:CDS 35.0%
! CTTTACCAGCAACATTTTCA+GGG - chr4.3:6713806-6713825 None:intergenic 35.0%
! GATGAAACCGTTAACCATTT+TGG + chr4.3:6714108-6714127 MS.gene000539:CDS 35.0%
! GCATCCAAAATCTTTTTGCT+AGG - chr4.3:6715150-6715169 None:intergenic 35.0%
! TCCTTTTCTTACCACCATAT+TGG + chr4.3:6713742-6713761 MS.gene000539:CDS 35.0%
! TTGGTAGGACACAAACTTTT+GGG + chr4.3:6714399-6714418 MS.gene000539:intron 35.0%
! TTTGGTAGGACACAAACTTT+TGG + chr4.3:6714398-6714417 MS.gene000539:intron 35.0%
!! AAAGTCTTATTGACACCAGA+AGG + chr4.3:6714782-6714801 MS.gene000539:CDS 35.0%
!! TGGCCTTATTGAGTTGAATT+GGG + chr4.3:6714346-6714365 MS.gene000539:intron 35.0%
AAACAGCAGAGGCAAAATTG+TGG + chr4.3:6714462-6714481 MS.gene000539:CDS 40.0%
AAGGAAAAGAAGGTGAATGG+AGG - chr4.3:6713720-6713739 None:intergenic 40.0%
ACGCCTACAATCATCAACTT+TGG - chr4.3:6714082-6714101 None:intergenic 40.0%
AGTGTTGTGTCCAGAATTGT+TGG + chr4.3:6715065-6715084 MS.gene000539:CDS 40.0%
ATCTTCAATTCGATCTCCTG+AGG + chr4.3:6715197-6715216 MS.gene000539:CDS 40.0%
ATTGACACCAGAAGGCAAAA+TGG + chr4.3:6714790-6714809 MS.gene000539:CDS 40.0%
GAAACATTGAGGGTTGAAGT+TGG + chr4.3:6714698-6714717 MS.gene000539:CDS 40.0%
GGTAAAGTTGTTCACATCAC+CGG + chr4.3:6713819-6713838 MS.gene000539:CDS 40.0%
GGTGTAACAATTGTTACACC+AGG + chr4.3:6714731-6714750 MS.gene000539:CDS 40.0%
TCAGGAGATCGAATTGAAGA+TGG - chr4.3:6715198-6715217 None:intergenic 40.0%
TGAGGTGTGGTAAAGCAAAA+CGG - chr4.3:6714441-6714460 None:intergenic 40.0%
TGGTGGTAAGAAAAGGAGTA+AGG - chr4.3:6713739-6713758 None:intergenic 40.0%
TTCTTCCCTGAAAATGTTGC+TGG + chr4.3:6713798-6713817 MS.gene000539:CDS 40.0%
TTGACACCAGAAGGCAAAAT+GGG + chr4.3:6714791-6714810 MS.gene000539:CDS 40.0%
! TGGTAGGACACAAACTTTTG+GGG + chr4.3:6714400-6714419 MS.gene000539:intron 40.0%
! TTTTACCTCTCCAATACCAG+AGG - chr4.3:6713850-6713869 None:intergenic 40.0%
!! AGTTGATGATTGTAGGCGTT+TGG + chr4.3:6714083-6714102 MS.gene000539:CDS 40.0%
!! CTGGCCTTATTGAGTTGAAT+TGG + chr4.3:6714345-6714364 MS.gene000539:intron 40.0%
AAACCCCACTCCAACAATTC+TGG - chr4.3:6715078-6715097 None:intergenic 45.0%
AGTAAACACTACTACCCTGC+AGG + chr4.3:6714646-6714665 MS.gene000539:intron 45.0%
ATGGTACATTGAGGGGAGAT+AGG + chr4.3:6714998-6715017 MS.gene000539:CDS 45.0%
GAGGTGTGGTAAAGCAAAAC+GGG - chr4.3:6714440-6714459 None:intergenic 45.0%
GCTTACGAGTATGCTAAGAG+AGG + chr4.3:6713949-6713968 MS.gene000539:CDS 45.0%
GTGTCCAGAATTGTTGGAGT+GGG + chr4.3:6715071-6715090 MS.gene000539:CDS 45.0%
TACCATGAGAGGTCTCCAAT+TGG - chr4.3:6714299-6714318 None:intergenic 45.0%
TATATACTCCTTCTGGGTGC+AGG - chr4.3:6714514-6714533 None:intergenic 45.0%
TGTCCAGAATTGTTGGAGTG+GGG + chr4.3:6715072-6715091 MS.gene000539:CDS 45.0%
TGTGTCCAGAATTGTTGGAG+TGG + chr4.3:6715070-6715089 MS.gene000539:CDS 45.0%
! GCTGATAGAGCTCGTGATTT+TGG + chr4.3:6714021-6714040 MS.gene000539:CDS 45.0%
!!! TTTTGCCTCTGCTGTTTCTG+AGG - chr4.3:6714459-6714478 None:intergenic 45.0%
AGGTGAATGGAGGAGCTACT+AGG - chr4.3:6713710-6713729 None:intergenic 50.0%
ATGGAGGAGCTACTAGGTTC+AGG - chr4.3:6713704-6713723 None:intergenic 50.0%
CACCAATTGGAGACCTCTCA+TGG + chr4.3:6714294-6714313 MS.gene000539:CDS 50.0%
CATTCACTCACTGATCCCAC+TGG - chr4.3:6714961-6714980 None:intergenic 50.0%
GAAGTGAGTGCAACTCCAGT+GGG + chr4.3:6714943-6714962 MS.gene000539:CDS 50.0%
GGTATTTGACTGCACCAGCA+TGG + chr4.3:6715019-6715038 MS.gene000539:CDS 50.0%
GTTGAAGTTGGAGCAGACGT+AGG + chr4.3:6714710-6714729 MS.gene000539:CDS 50.0%
TGAAGTGAGTGCAACTCCAG+TGG + chr4.3:6714942-6714961 MS.gene000539:CDS 50.0%
TTGCTAGGTGCTTCTCTTGC+TGG - chr4.3:6715135-6715154 None:intergenic 50.0%
! TCCAATACCAGAGGAAGCAC+CGG - chr4.3:6713841-6713860 None:intergenic 50.0%
! TGCTTCCTCTGGTATTGGAG+AGG + chr4.3:6713842-6713861 MS.gene000539:CDS 50.0%
ACCAGAAGGCAAAATGGGCG+TGG + chr4.3:6714796-6714815 MS.gene000539:CDS 55.0%
ACCGGTGCTTCCTCTGGTAT+TGG + chr4.3:6713837-6713856 MS.gene000539:CDS 55.0%
CCACACCTCAGAAACAGCAG+AGG + chr4.3:6714451-6714470 MS.gene000539:CDS 55.0%
! TCCACGCCCATTTTGCCTTC+TGG - chr4.3:6714800-6714819 None:intergenic 55.0%
!! ATGCTGCTTTGCTTGCCTGC+AGG - chr4.3:6714664-6714683 None:intergenic 55.0%
!! CCTCTGCTGTTTCTGAGGTG+TGG - chr4.3:6714454-6714473 None:intergenic 55.0%
!! TGCTGCTTTGCTTGCCTGCA+GGG - chr4.3:6714663-6714682 None:intergenic 55.0%
CACATCACCGGTGCTTCCTC+TGG + chr4.3:6713831-6713850 MS.gene000539:CDS 60.0%
CTTGGATGCCTGCACCCAGA+AGG + chr4.3:6714503-6714522 MS.gene000539:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 6713681 6715233 6713681 ID=MS.gene000539
chr4.3 mRNA 6713681 6715233 6713681 ID=MS.gene000539.t1;Parent=MS.gene000539
chr4.3 exon 6713681 6713863 6713681 ID=MS.gene000539.t1.exon1;Parent=MS.gene000539.t1
chr4.3 CDS 6713681 6713863 6713681 ID=cds.MS.gene000539.t1;Parent=MS.gene000539.t1
chr4.3 exon 6713943 6714135 6713943 ID=MS.gene000539.t1.exon2;Parent=MS.gene000539.t1
chr4.3 CDS 6713943 6714135 6713943 ID=cds.MS.gene000539.t1;Parent=MS.gene000539.t1
chr4.3 exon 6714230 6714315 6714230 ID=MS.gene000539.t1.exon3;Parent=MS.gene000539.t1
chr4.3 CDS 6714230 6714315 6714230 ID=cds.MS.gene000539.t1;Parent=MS.gene000539.t1
chr4.3 exon 6714406 6714537 6714406 ID=MS.gene000539.t1.exon4;Parent=MS.gene000539.t1
chr4.3 CDS 6714406 6714537 6714406 ID=cds.MS.gene000539.t1;Parent=MS.gene000539.t1
chr4.3 exon 6714668 6714835 6714668 ID=MS.gene000539.t1.exon5;Parent=MS.gene000539.t1
chr4.3 CDS 6714668 6714835 6714668 ID=cds.MS.gene000539.t1;Parent=MS.gene000539.t1
chr4.3 exon 6714940 6715233 6714940 ID=MS.gene000539.t1.exon6;Parent=MS.gene000539.t1
chr4.3 CDS 6714940 6715233 6714940 ID=cds.MS.gene000539.t1;Parent=MS.gene000539.t1
Gene Sequence

>MS.gene000539

ATGGACCTTATTCATAAGTTCCTGAACCTAGTAGCTCCTCCATTCACCTTCTTTTCCTTACTCCTTTTCTTACCACCATATTGGACCTTCAAATTCTTCCTTTCCATTATCTTTACATTCTTCCCTGAAAATGTTGCTGGTAAAGTTGTTCACATCACCGGTGCTTCCTCTGGTATTGGAGAGGTAAAACATATAGTATTCTCAATTAATTCATATATACACTAACTAACATTATTATATTTAATGATTTTAATTTTTGTAGCATTTGGCTTACGAGTATGCTAAGAGAGGTGCACGATTAGCACTTTCTGCTAGAAGAGACACAGCACTGCGTGAAGTTGCTGATAGAGCTCGTGATTTTGGTTCTCCTGATGTTATTATAATGCGTGCAGATGTTTCCAAAGTTGATGATTGTAGGCGTTTGGTCGATGAAACCGTTAACCATTTTGGTAGACGTATGTATGTGACAGTCAAAGTTTCCTTAAACAAATATTAATTAATCACATAAATACATATCTTATACTGTTAATTCTCTGTAATGCATTACAGTGGATCATTTGGTAAACAATGCTGCAATCAGTGCTGCCATGATGTTTGAAGATGTAACTGATATCACCAATTGGAGACCTCTCATGGTAATTTAAAGCTAACTACATGACTTTATCTGGCCTTATTGAGTTGAATTGGGAATTGGCTAATTATTTATTTGCTGTGTATTTTGGTAGGACACAAACTTTTGGGGATCAGTTTACACAACCCGTTTTGCTTTACCACACCTCAGAAACAGCAGAGGCAAAATTGTGGTCCTTTCATCAATTGATTCTTGGATGCCTGCACCCAGAAGGAGTATATATAATGTACGTACTAAGTGACTGACTGATATATTACTATTATAATCTATCTCTATTTTCAATTAATATTGTTCAATATATTATATTCTTCATATTATTTCATTTAATTATTAAAGTAAACACTACTACCCTGCAGGCAAGCAAAGCAGCATTAGTAAGTCTTTATGAAACATTGAGGGTTGAAGTTGGAGCAGACGTAGGTGTAACAATTGTTACACCAGGTTACATAGAATCTGAATTAACCAAAGGCAAAGTCTTATTGACACCAGAAGGCAAAATGGGCGTGGACCAAGATATGAGAGATGTAACAAAATTTTCAATCTCATCTTTTAATTTACCTAGTAATACAGGCTATAACTATTCATATATATAATTTACATAATTAATGTTATGATGATATATATGTAGGTTGAAGTGAGTGCAACTCCAGTGGGATCAGTGAGTGAATGTGCAAAATCAATAGTGAATGGTACATTGAGGGGAGATAGGTATTTGACTGCACCAGCATGGTTTAGAATGACATATGTTGTGAAAGTGTTGTGTCCAGAATTGTTGGAGTGGGGTTTCAGAATGTTGTACTTGACTAGATCAAGTAATACTCCAGCAAGAGAAGCACCTAGCAAAAAGATTTTGGATGCTACTGGAATCAAAAACGTGTTCTATCCATCTTCAATTCGATCTCCTGAGGTCAAGACTGAATGA
Protein sequence

>MS.gene000539.t1

MDLIHKFLNLVAPPFTFFSLLLFLPPYWTFKFFLSIIFTFFPENVAGKVVHITGASSGIGEHLAYEYAKRGARLALSARRDTALREVADRARDFGSPDVIIMRADVSKVDDCRRLVDETVNHFGRLDHLVNNAAISAAMMFEDVTDITNWRPLMDTNFWGSVYTTRFALPHLRNSRGKIVVLSSIDSWMPAPRRSIYNASKAALVSLYETLRVEVGADVGVTIVTPGYIESELTKGKVLLTPEGKMGVDQDMRDVEVSATPVGSVSECAKSIVNGTLRGDRYLTAPAWFRMTYVVKVLCPELLEWGFRMLYLTRSSNTPAREAPSKKILDATGIKNVFYPSSIRSPEVKTE