AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00059

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00059.t1 MTR_2g031990 92.500 280 10 1 1 269 1 280 0.0 514
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00059.t1 AT4G20760 71.765 255 61 1 26 269 44 298 2.30e-132 376
MS.gene00059.t1 AT4G20760 71.765 255 61 1 26 269 64 318 1.33e-131 375

Find 65 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGATGTGCAACAACTTT+TGG 0.278163 2.1:-60032972 None:intergenic
AACCTTCTCATTAATGCATC+TGG 0.304289 2.1:+60031790 MS.gene00059:CDS
TGATGAGGAGAAGGCTCTTC+TGG 0.317797 2.1:-60030214 None:intergenic
CCTTAGTTCAAGGAGCTTCC+CGG 0.325312 2.1:+60030262 MS.gene00059:CDS
AGAAACCACGTTGAACAAAT+TGG 0.325708 2.1:+60032103 MS.gene00059:intron
TTACCTGGTTGCAATACTTC+AGG 0.348845 2.1:-60031826 None:intergenic
CAGTTGATGCATTAGGATTA+CGG 0.378721 2.1:-60030557 None:intergenic
AGTGCAAGGGTTGCATCTAT+TGG 0.379178 2.1:+60032430 MS.gene00059:CDS
CATTAATGAGAAGGTTTAGA+TGG 0.381244 2.1:-60031783 None:intergenic
CGTAATCCTAATGCATCAAC+TGG 0.384959 2.1:+60030558 MS.gene00059:CDS
AACTGGGCTTCTTCATTTGA+AGG 0.387513 2.1:+60030575 MS.gene00059:CDS
AGAAGCCCAGTTGATGCATT+AGG 0.400206 2.1:-60030564 None:intergenic
CTTAAAGCTGGAGGTGGCAT+TGG 0.412070 2.1:+60032373 MS.gene00059:CDS
CTCTTCTTAGTTCTTAAAGC+TGG 0.422620 2.1:+60032361 MS.gene00059:intron
AACGGTGTTTCCTTAGTTCA+AGG 0.422805 2.1:+60030252 MS.gene00059:CDS
CTCTTCTGGCTAGTGATAGA+AGG 0.423027 2.1:-60030200 None:intergenic
TGCAATAGAATACATATGAT+TGG 0.426249 2.1:-60032865 None:intergenic
CAATGGCAAGTTCTTTGCTT+GGG 0.439897 2.1:+60033014 MS.gene00059:CDS
CCGGGAAGCTCCTTGAACTA+AGG 0.441207 2.1:-60030262 None:intergenic
CAAGGAGCTTCCCGGGGAAT+CGG 0.443539 2.1:+60030270 MS.gene00059:CDS
CTTAGTTCAAGGAGCTTCCC+GGG 0.449701 2.1:+60030263 MS.gene00059:CDS
TTAAAGCTGGAGGTGGCATT+GGG 0.450662 2.1:+60032374 MS.gene00059:CDS
CGAATTCAAGACCGATTCCC+CGG 0.453887 2.1:-60030281 None:intergenic
TTGTGGCTAGTTTGAGTGCA+AGG 0.455555 2.1:+60032416 MS.gene00059:CDS
CTATTGGCGATAATCGTCTC+GGG 0.464427 2.1:+60032446 MS.gene00059:CDS
GGCAAGTTCTTTGCTTGGGA+TGG 0.471669 2.1:+60033018 MS.gene00059:CDS
TTCTTAGTTCTTAAAGCTGG+AGG 0.480961 2.1:+60032364 MS.gene00059:intron
ACAATGGCAAGTTCTTTGCT+TGG 0.481131 2.1:+60033013 MS.gene00059:CDS
GTCTTTAATGCTTGCATGTG+AGG 0.486993 2.1:+60032133 MS.gene00059:CDS
GACCGTTGAAAGTTCTATTG+AGG 0.487265 2.1:+60030638 MS.gene00059:CDS
GGGATGGTCAAGAAATTCCT+TGG 0.508072 2.1:+60033034 MS.gene00059:CDS
ACCTTCTCATTAATGCATCT+GGG 0.510117 2.1:+60031791 MS.gene00059:CDS
AGATCTGTGTCAACTGTACC+AGG 0.510748 2.1:-60032889 None:intergenic
GTTGGAGTTTGCGAGGAAGA+AGG 0.522651 2.1:+60032840 MS.gene00059:CDS
TTGTCAATAAAAGAGACATA+TGG 0.523455 2.1:+60031760 MS.gene00059:CDS
AGAAGAAGATGATGAGGAGA+AGG 0.532540 2.1:-60030223 None:intergenic
GGAAAGAAGTGGTGCAGTTG+TGG 0.541874 2.1:+60032399 MS.gene00059:CDS
TCCCAGATGCATTAATGAGA+AGG 0.541960 2.1:-60031792 None:intergenic
TTAGTTCTTAAAGCTGGAGG+TGG 0.543531 2.1:+60032367 MS.gene00059:intron
TCTATTGGCGATAATCGTCT+CGG 0.545892 2.1:+60032445 MS.gene00059:CDS
GCGAGGGGAACGTGAAGCCA+TGG 0.552409 2.1:-60030178 None:intergenic
TGTGGCTAGTTTGAGTGCAA+GGG 0.561568 2.1:+60032417 MS.gene00059:CDS
AGCTGGAGGTGGCATTGGGA+CGG 0.573880 2.1:+60032378 MS.gene00059:CDS
TGGCTAGTGATAGAAGGCGA+GGG 0.574948 2.1:-60030194 None:intergenic
ATATGTATTCTATTGCATCC+TGG 0.575422 2.1:+60032871 MS.gene00059:CDS
AGCTTGAGAAGAAGATGATG+AGG 0.580076 2.1:-60030229 None:intergenic
GCATGTGAGGTTAATGCCGT+CGG 0.582444 2.1:+60032146 MS.gene00059:CDS
AACCTCAATAGAACTTTCAA+CGG 0.590126 2.1:-60030640 None:intergenic
GAATTCAAGACCGATTCCCC+GGG 0.593369 2.1:-60030280 None:intergenic
CAAAGAACTTGCCATTGTCA+TGG 0.596876 2.1:-60033008 None:intergenic
CGATCTCGTTGGAGTTTGCG+AGG 0.597005 2.1:+60032833 MS.gene00059:CDS
CTGGCTAGTGATAGAAGGCG+AGG 0.602623 2.1:-60030195 None:intergenic
TCATCTTCTTCTCAAGCTAA+CGG 0.605419 2.1:+60030234 MS.gene00059:CDS
TATTGGCGATAATCGTCTCG+GGG 0.615665 2.1:+60032447 MS.gene00059:CDS
CTTCTCCAATTTGTTCAACG+TGG 0.620245 2.1:-60032108 None:intergenic
CTTTCAACGGTCAAATCCAA+AGG 0.625409 2.1:-60030627 None:intergenic
AGTGTCAAAGACGATCTCGT+TGG 0.626216 2.1:+60032822 MS.gene00059:intron
GGCATTGGGACGGAAAGAAG+TGG 0.627744 2.1:+60032388 MS.gene00059:CDS
TTAGTTCAAGGAGCTTCCCG+GGG 0.646314 2.1:+60030264 MS.gene00059:CDS
GGCTAGTGATAGAAGGCGAG+GGG 0.647176 2.1:-60030193 None:intergenic
AATGTCAAGAGCCATGACAA+TGG 0.667426 2.1:+60032997 MS.gene00059:CDS
ACAAATATGACAGATTACCA+AGG 0.689876 2.1:-60033051 None:intergenic
GTAATCCTAATGCATCAACT+GGG 0.689940 2.1:+60030559 MS.gene00059:CDS
ATACCTGAAGTATTGCAACC+AGG 0.700492 2.1:+60031823 MS.gene00059:CDS
ATTGGCGATAATCGTCTCGG+GGG 0.707865 2.1:+60032448 MS.gene00059:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAACTTATAGATTATA+TGG + chr2.1:60032702-60032721 MS.gene00059:intron 10.0%
!!! ATAATTTTAGAAGTAAAATT+GGG + chr2.1:60032241-60032260 MS.gene00059:intron 10.0%
!! TAGTTTATGTAAAAGATAAA+AGG - chr2.1:60031293-60031312 None:intergenic 15.0%
!! TTGTGTATTTATAATATTTC+AGG + chr2.1:60031729-60031748 MS.gene00059:intron 15.0%
!!! AAAAAATTTACTTTTCTTCA+TGG + chr2.1:60030353-60030372 MS.gene00059:intron 15.0%
!!! AAAAATTTACTTTTCTTCAT+GGG + chr2.1:60030354-60030373 MS.gene00059:intron 15.0%
!!! GATAATTTTAGAAGTAAAAT+TGG + chr2.1:60032240-60032259 MS.gene00059:intron 15.0%
!!! TAAAAATTTGTTTCTTTCTT+TGG + chr2.1:60031846-60031865 MS.gene00059:intron 15.0%
!!! TTAGCAATATTTTTTTGAAA+TGG - chr2.1:60032922-60032941 None:intergenic 15.0%
!!! TTTAGCTAATATTTAACTTT+AGG + chr2.1:60030820-60030839 MS.gene00059:intron 15.0%
!! AAGCACTTATAGAATTATTT+GGG + chr2.1:60032610-60032629 MS.gene00059:intron 20.0%
!! ATATGATAAACTGTTTGAAA+TGG + chr2.1:60032763-60032782 MS.gene00059:intron 20.0%
!! CTTATCATATTGAATATAAC+AGG - chr2.1:60032542-60032561 None:intergenic 20.0%
!! TTAAACAAGATAGAAAGAAA+CGG + chr2.1:60031959-60031978 MS.gene00059:intron 20.0%
!!! AAAATTTACTTTTCTTCATG+GGG + chr2.1:60030355-60030374 MS.gene00059:intron 20.0%
!!! AAACAAAATATGTCTCATTT+TGG + chr2.1:60031042-60031061 MS.gene00059:intron 20.0%
!!! ATGTAAAAGATAAAAGGTAT+TGG - chr2.1:60031287-60031306 None:intergenic 20.0%
!!! TGTAAAAGATAAAAGGTATT+GGG - chr2.1:60031286-60031305 None:intergenic 20.0%
!!! TGTCATTTTATTTTTAGTCT+AGG + chr2.1:60030378-60030397 MS.gene00059:intron 20.0%
!!! TTAACAAATGAATAGGTTTT+TGG - chr2.1:60031706-60031725 None:intergenic 20.0%
!!! TTGTGTTTTGTATTGTATAT+AGG + chr2.1:60030485-60030504 MS.gene00059:intron 20.0%
! ACAAAGCTTAACAAATGAAT+AGG - chr2.1:60031713-60031732 None:intergenic 25.0%
! ATTTCTGATACAACTAAGAA+TGG - chr2.1:60031233-60031252 None:intergenic 25.0%
! GAATACATGAAGAAAAACTT+AGG - chr2.1:60031898-60031917 None:intergenic 25.0%
! TAATGCTATGTCATCAAAAT+GGG + chr2.1:60031311-60031330 MS.gene00059:intron 25.0%
! TGAATATGCTATTAGTACTT+GGG + chr2.1:60030845-60030864 MS.gene00059:intron 25.0%
! TGCAATAGAATACATATGAT+TGG - chr2.1:60032868-60032887 None:intergenic 25.0%
! TGCTAAAGTTAATATGGATA+TGG + chr2.1:60030760-60030779 MS.gene00059:intron 25.0%
! TTGAATATGCTATTAGTACT+TGG + chr2.1:60030844-60030863 MS.gene00059:intron 25.0%
! TTGTCAATAAAAGAGACATA+TGG + chr2.1:60031760-60031779 MS.gene00059:CDS 25.0%
!! ATGTTATAAGCTATTTCCAT+AGG - chr2.1:60032640-60032659 None:intergenic 25.0%
!! GAAAGAAACAAATTTTTACC+TGG - chr2.1:60031844-60031863 None:intergenic 25.0%
!! GAAGCACTTATAGAATTATT+TGG + chr2.1:60032609-60032628 MS.gene00059:intron 25.0%
!! TTTGTTTCTTTCTTTGGTAA+TGG + chr2.1:60031852-60031871 MS.gene00059:intron 25.0%
!!! TTCTAGTCTTGTAACTAAAA+GGG - chr2.1:60030734-60030753 None:intergenic 25.0%
AACATCAGTCTTGATAGAAA+AGG - chr2.1:60031655-60031674 None:intergenic 30.0%
AACATTGGACATAATTGTTG+TGG + chr2.1:60030420-60030439 MS.gene00059:intron 30.0%
AACCTCAATAGAACTTTCAA+CGG - chr2.1:60030643-60030662 None:intergenic 30.0%
AACTAAAAACTCTATAGCAC+CGG - chr2.1:60030459-60030478 None:intergenic 30.0%
AATTTCTAAATCAGCACATG+TGG + chr2.1:60032337-60032356 MS.gene00059:intron 30.0%
ATATGTATTCTATTGCATCC+TGG + chr2.1:60032871-60032890 MS.gene00059:CDS 30.0%
ATGAAGAAAAACTTAGGTGA+TGG - chr2.1:60031892-60031911 None:intergenic 30.0%
ATGAGATTATAGAAGCCTTA+GGG + chr2.1:60032276-60032295 MS.gene00059:intron 30.0%
CAATATGATAAGTGCATCAT+TGG + chr2.1:60032550-60032569 MS.gene00059:intron 30.0%
CATTAATGAGAAGGTTTAGA+TGG - chr2.1:60031786-60031805 None:intergenic 30.0%
CTAAAAACGGAACAATCAAA+AGG + chr2.1:60031935-60031954 MS.gene00059:intron 30.0%
CTAATGCTATGTCATCAAAA+TGG + chr2.1:60031310-60031329 MS.gene00059:intron 30.0%
GGTAGATGCTAAAGTTAATA+TGG + chr2.1:60030754-60030773 MS.gene00059:intron 30.0%
TAATGTGGCAAGATTTCATA+TGG - chr2.1:60031180-60031199 None:intergenic 30.0%
TAGATATGAGATTTGTGACA+AGG + chr2.1:60030784-60030803 MS.gene00059:intron 30.0%
TATGAGATTATAGAAGCCTT+AGG + chr2.1:60032275-60032294 MS.gene00059:intron 30.0%
TGACACTGTTTGAATATAAG+CGG - chr2.1:60032809-60032828 None:intergenic 30.0%
TTATATCCTCGAACAATCTT+TGG + chr2.1:60030942-60030961 MS.gene00059:intron 30.0%
TTGTTCTACGATATGCTATT+TGG - chr2.1:60031504-60031523 None:intergenic 30.0%
! ACAATTTGTTCACTATGCTA+CGG + chr2.1:60031436-60031455 MS.gene00059:intron 30.0%
! GGCAAGATTTTATTCTAATG+TGG - chr2.1:60031195-60031214 None:intergenic 30.0%
! TAATGATGTGCAACAACTTT+TGG - chr2.1:60032975-60032994 None:intergenic 30.0%
!! AAAAGATAAAAGGTATTGGG+TGG - chr2.1:60031283-60031302 None:intergenic 30.0%
!! CTTCTAGTCTTGTAACTAAA+AGG - chr2.1:60030735-60030754 None:intergenic 30.0%
!! CTTTTAGTTACAAGACTAGA+AGG + chr2.1:60030733-60030752 MS.gene00059:intron 30.0%
AACCTTCTCATTAATGCATC+TGG + chr2.1:60031790-60031809 MS.gene00059:CDS 35.0%
AATGCTACATTGGATAAGTG+AGG + chr2.1:60031129-60031148 MS.gene00059:intron 35.0%
ACACTTCAACAATGCTACAT+TGG + chr2.1:60031119-60031138 MS.gene00059:intron 35.0%
ACCTTCTCATTAATGCATCT+GGG + chr2.1:60031791-60031810 MS.gene00059:CDS 35.0%
ACTGCTCAGCATAGTAAATA+AGG - chr2.1:60031389-60031408 None:intergenic 35.0%
AGAAACCACGTTGAACAAAT+TGG + chr2.1:60032103-60032122 MS.gene00059:intron 35.0%
AGGAGGATTAACAAATGTAG+TGG + chr2.1:60031629-60031648 MS.gene00059:intron 35.0%
ATGTGAGAAATGCCAAACAT+TGG - chr2.1:60030329-60030348 None:intergenic 35.0%
CTGCTCAGCATAGTAAATAA+GGG - chr2.1:60031388-60031407 None:intergenic 35.0%
GAATTATTTGGGAGAACCTA+TGG + chr2.1:60032621-60032640 MS.gene00059:intron 35.0%
GAGTGTTCAACTGTATAGAT+GGG + chr2.1:60032027-60032046 MS.gene00059:intron 35.0%
GATACAACTAAGAATGGCAA+TGG - chr2.1:60031227-60031246 None:intergenic 35.0%
GTAATCCTAATGCATCAACT+GGG + chr2.1:60030559-60030578 MS.gene00059:CDS 35.0%
TCATCTTCTTCTCAAGCTAA+CGG + chr2.1:60030234-60030253 MS.gene00059:CDS 35.0%
TGCAATATCCTTTATGCTCA+AGG - chr2.1:60031357-60031376 None:intergenic 35.0%
TTCATATGGCAGCTACTAAT+TGG - chr2.1:60031166-60031185 None:intergenic 35.0%
! AGAGTGTTCAACTGTATAGA+TGG + chr2.1:60032026-60032045 MS.gene00059:intron 35.0%
! CAGTTGATGCATTAGGATTA+CGG - chr2.1:60030560-60030579 None:intergenic 35.0%
! CTCTTCTTAGTTCTTAAAGC+TGG + chr2.1:60032361-60032380 MS.gene00059:intron 35.0%
! GTTACCTTGATAACCAAGAT+AGG - chr2.1:60032173-60032192 None:intergenic 35.0%
! TATAGATGGGAGTGTGTAAT+GGG + chr2.1:60032040-60032059 MS.gene00059:intron 35.0%
! TCGACTTCAAATTTTGCCTT+TGG + chr2.1:60030611-60030630 MS.gene00059:CDS 35.0%
! TTAGACACCAAGTTGAGATT+TGG + chr2.1:60030705-60030724 MS.gene00059:intron 35.0%
! TTCTTAGTTCTTAAAGCTGG+AGG + chr2.1:60032364-60032383 MS.gene00059:intron 35.0%
!! ACAAGTGGGTTAACTTAATC+AGG - chr2.1:60031582-60031601 None:intergenic 35.0%
!! CAAGTGGGTTAACTTAATCA+GGG - chr2.1:60031581-60031600 None:intergenic 35.0%
!! CAGCTTTAAGAACTAAGAAG+AGG - chr2.1:60032363-60032382 None:intergenic 35.0%
!!! AATGCCAAACATTGGTTTTG+AGG - chr2.1:60030321-60030340 None:intergenic 35.0%
!!! ATGCCAAACATTGGTTTTGA+GGG - chr2.1:60030320-60030339 None:intergenic 35.0%
AACGGTGTTTCCTTAGTTCA+AGG + chr2.1:60030252-60030271 MS.gene00059:CDS 40.0%
AACTGGGCTTCTTCATTTGA+AGG + chr2.1:60030575-60030594 MS.gene00059:CDS 40.0%
AATGTCAAGAGCCATGACAA+TGG + chr2.1:60032997-60033016 MS.gene00059:CDS 40.0%
ACAATGGCAAGTTCTTTGCT+TGG + chr2.1:60033013-60033032 MS.gene00059:CDS 40.0%
AGAAGAAGATGATGAGGAGA+AGG - chr2.1:60030226-60030245 None:intergenic 40.0%
AGCTTGAGAAGAAGATGATG+AGG - chr2.1:60030232-60030251 None:intergenic 40.0%
ATCCTCGAACAATCTTTGGT+TGG + chr2.1:60030946-60030965 MS.gene00059:intron 40.0%
CAAAGAACTTGCCATTGTCA+TGG - chr2.1:60033011-60033030 None:intergenic 40.0%
CAATGGCAAGTTCTTTGCTT+GGG + chr2.1:60033014-60033033 MS.gene00059:CDS 40.0%
CCATTTCAGAACTGACAAGT+GGG - chr2.1:60031596-60031615 None:intergenic 40.0%
CGTAATCCTAATGCATCAAC+TGG + chr2.1:60030558-60030577 MS.gene00059:CDS 40.0%
CTCAGCATAGTAAATAAGGG+TGG - chr2.1:60031385-60031404 None:intergenic 40.0%
CTTGAGTCTAACCACATAGA+AGG - chr2.1:60030880-60030899 None:intergenic 40.0%
CTTTCAACGGTCAAATCCAA+AGG - chr2.1:60030630-60030649 None:intergenic 40.0%
GAATGGCAATGGTTATAGTG+AGG - chr2.1:60031216-60031235 None:intergenic 40.0%
GACATAATTGTTGTGGACAC+CGG + chr2.1:60030427-60030446 MS.gene00059:intron 40.0%
GACCGTTGAAAGTTCTATTG+AGG + chr2.1:60030638-60030657 MS.gene00059:CDS 40.0%
GACTGATGTTAGACATCTCA+TGG + chr2.1:60031665-60031684 MS.gene00059:intron 40.0%
GGAATATGTCTCCTTCTATG+TGG + chr2.1:60030866-60030885 MS.gene00059:intron 40.0%
GTCTTTAATGCTTGCATGTG+AGG + chr2.1:60032133-60032152 MS.gene00059:CDS 40.0%
GTTGCAGATTTGCGAGAAAA+AGG + chr2.1:60031993-60032012 MS.gene00059:intron 40.0%
TCAGAAATTCAGCATTCAGC+AGG + chr2.1:60031245-60031264 MS.gene00059:intron 40.0%
TCCCAGATGCATTAATGAGA+AGG - chr2.1:60031795-60031814 None:intergenic 40.0%
TCTACAAGCCTTGAGCATAA+AGG + chr2.1:60031346-60031365 MS.gene00059:intron 40.0%
TCTATGTGGTTAGACTCAAG+TGG + chr2.1:60030880-60030899 MS.gene00059:intron 40.0%
TCTATTGGCGATAATCGTCT+CGG + chr2.1:60032445-60032464 MS.gene00059:CDS 40.0%
TCTCCCTCAAAACCAATGTT+TGG + chr2.1:60030314-60030333 MS.gene00059:intron 40.0%
TTACCTGGTTGCAATACTTC+AGG - chr2.1:60031829-60031848 None:intergenic 40.0%
! ATAGATGGGAGTGTGTAATG+GGG + chr2.1:60032041-60032060 MS.gene00059:intron 40.0%
! CTTCTCCAATTTGTTCAACG+TGG - chr2.1:60032111-60032130 None:intergenic 40.0%
! CTTTTGGACTGAGTACTCTT+TGG - chr2.1:60032959-60032978 None:intergenic 40.0%
! GAAATGGGAACTTTTGTCTC+AGG + chr2.1:60031609-60031628 MS.gene00059:intron 40.0%
! GTATAGATGGGAGTGTGTAA+TGG + chr2.1:60032039-60032058 MS.gene00059:intron 40.0%
! TTAGTTCTTAAAGCTGGAGG+TGG + chr2.1:60032367-60032386 MS.gene00059:intron 40.0%
!! ATACCTGAAGTATTGCAACC+AGG + chr2.1:60031823-60031842 MS.gene00059:CDS 40.0%
!! CCACTTGTCAGTTCTGAAAT+GGG + chr2.1:60031594-60031613 MS.gene00059:intron 40.0%
!!! TCCGTTTTTAGTGCATGCAA+GGG - chr2.1:60031926-60031945 None:intergenic 40.0%
!!! TTCCGTTTTTAGTGCATGCA+AGG - chr2.1:60031927-60031946 None:intergenic 40.0%
ACTGACACAGACACGAACAT+TGG + chr2.1:60030405-60030424 MS.gene00059:intron 45.0%
AGATCTGTGTCAACTGTACC+AGG - chr2.1:60032892-60032911 None:intergenic 45.0%
AGGGTCACCAAATCTCAACT+TGG - chr2.1:60030715-60030734 None:intergenic 45.0%
AGTGCAAGGGTTGCATCTAT+TGG + chr2.1:60032430-60032449 MS.gene00059:CDS 45.0%
AGTGTCAAAGACGATCTCGT+TGG + chr2.1:60032822-60032841 MS.gene00059:intron 45.0%
ATCTTAACCTCCCAACACCA+AGG - chr2.1:60031019-60031038 None:intergenic 45.0%
CCCATTTCAGAACTGACAAG+TGG - chr2.1:60031597-60031616 None:intergenic 45.0%
CCCCTTGCATGCACTAAAAA+CGG + chr2.1:60031922-60031941 MS.gene00059:intron 45.0%
CGGTCCTATCTTGGTTATCA+AGG + chr2.1:60032166-60032185 MS.gene00059:intron 45.0%
CTATTGGCGATAATCGTCTC+GGG + chr2.1:60032446-60032465 MS.gene00059:CDS 45.0%
GACTCAAACTACATGCCCTA+AGG - chr2.1:60032294-60032313 None:intergenic 45.0%
GATAACCAAGATAGGACCGA+CGG - chr2.1:60032165-60032184 None:intergenic 45.0%
GGAAAGGGGTTGTAGTAATC+TGG - chr2.1:60030997-60031016 None:intergenic 45.0%
GGGATGGTCAAGAAATTCCT+TGG + chr2.1:60033034-60033053 MS.gene00059:CDS 45.0%
GTCCAACCAAAGATTGTTCG+AGG - chr2.1:60030951-60030970 None:intergenic 45.0%
TATTGGCGATAATCGTCTCG+GGG + chr2.1:60032447-60032466 MS.gene00059:CDS 45.0%
TCTTAACCTCCCAACACCAA+GGG - chr2.1:60031018-60031037 None:intergenic 45.0%
TTAAAGCTGGAGGTGGCATT+GGG + chr2.1:60032374-60032393 MS.gene00059:CDS 45.0%
TTACTACAACCCCTTTCCCT+TGG + chr2.1:60030999-60031018 MS.gene00059:intron 45.0%
! AGAAGCCCAGTTGATGCATT+AGG - chr2.1:60030567-60030586 None:intergenic 45.0%
! ATGGGAACTTTTGTCTCAGG+AGG + chr2.1:60031612-60031631 MS.gene00059:intron 45.0%
! CTCTTCTGGCTAGTGATAGA+AGG - chr2.1:60030203-60030222 None:intergenic 45.0%
! TAGATGGGAGTGTGTAATGG+GGG + chr2.1:60032042-60032061 MS.gene00059:intron 45.0%
!! CCCACTTGTCAGTTCTGAAA+TGG + chr2.1:60031593-60031612 MS.gene00059:intron 45.0%
!! TGTGGCTAGTTTGAGTGCAA+GGG + chr2.1:60032417-60032436 MS.gene00059:CDS 45.0%
!! TTGTGGCTAGTTTGAGTGCA+AGG + chr2.1:60032416-60032435 MS.gene00059:CDS 45.0%
!!! CCGTTTTTAGTGCATGCAAG+GGG - chr2.1:60031925-60031944 None:intergenic 45.0%
ATTGGCGATAATCGTCTCGG+GGG + chr2.1:60032448-60032467 MS.gene00059:CDS 50.0%
CCTTAGTTCAAGGAGCTTCC+CGG + chr2.1:60030262-60030281 MS.gene00059:CDS 50.0%
CTTAAAGCTGGAGGTGGCAT+TGG + chr2.1:60032373-60032392 MS.gene00059:CDS 50.0%
CTTAGTTCAAGGAGCTTCCC+GGG + chr2.1:60030263-60030282 MS.gene00059:CDS 50.0%
GCATGTGAGGTTAATGCCGT+CGG + chr2.1:60032146-60032165 MS.gene00059:CDS 50.0%
GGAAAGAAGTGGTGCAGTTG+TGG + chr2.1:60032399-60032418 MS.gene00059:CDS 50.0%
GGCAAGTTCTTTGCTTGGGA+TGG + chr2.1:60033018-60033037 MS.gene00059:CDS 50.0%
GTTGGAGTTTGCGAGGAAGA+AGG + chr2.1:60032840-60032859 MS.gene00059:CDS 50.0%
TAATGCCGTCGGTCCTATCT+TGG + chr2.1:60032157-60032176 MS.gene00059:intron 50.0%
TGATGAGGAGAAGGCTCTTC+TGG - chr2.1:60030217-60030236 None:intergenic 50.0%
TGGCTAGTGATAGAAGGCGA+GGG - chr2.1:60030197-60030216 None:intergenic 50.0%
TTAGTTCAAGGAGCTTCCCG+GGG + chr2.1:60030264-60030283 MS.gene00059:CDS 50.0%
TTTAGTGCATGCAAGGGGCA+TGG - chr2.1:60031920-60031939 None:intergenic 50.0%
! CGAATTCAAGACCGATTCCC+CGG - chr2.1:60030284-60030303 None:intergenic 50.0%
! GAATTCAAGACCGATTCCCC+GGG - chr2.1:60030283-60030302 None:intergenic 50.0%
! TCGGGGGTTGGCATTCATAT+CGG + chr2.1:60032464-60032483 MS.gene00059:CDS 50.0%
!! AACCCCTTTCCCTTGGTGTT+GGG + chr2.1:60031006-60031025 MS.gene00059:intron 50.0%
ACCTCCCAACACCAAGGGAA+AGG - chr2.1:60031013-60031032 None:intergenic 55.0%
CCGGGAAGCTCCTTGAACTA+AGG - chr2.1:60030265-60030284 None:intergenic 55.0%
CCTCCCAACACCAAGGGAAA+GGG - chr2.1:60031012-60031031 None:intergenic 55.0%
CGATCTCGTTGGAGTTTGCG+AGG + chr2.1:60032833-60032852 MS.gene00059:CDS 55.0%
CTCCCAACACCAAGGGAAAG+GGG - chr2.1:60031011-60031030 None:intergenic 55.0%
CTGGCTAGTGATAGAAGGCG+AGG - chr2.1:60030198-60030217 None:intergenic 55.0%
GGCTAGTGATAGAAGGCGAG+GGG - chr2.1:60030196-60030215 None:intergenic 55.0%
! CAACCCCTTTCCCTTGGTGT+TGG + chr2.1:60031005-60031024 MS.gene00059:intron 55.0%
! GGCATTGGGACGGAAAGAAG+TGG + chr2.1:60032388-60032407 MS.gene00059:CDS 55.0%
CAAGGAGCTTCCCGGGGAAT+CGG + chr2.1:60030270-60030289 MS.gene00059:CDS 60.0%
GCGATAATCGTCTCGGGGGT+TGG + chr2.1:60032452-60032471 MS.gene00059:CDS 60.0%
! AGCTGGAGGTGGCATTGGGA+CGG + chr2.1:60032378-60032397 MS.gene00059:CDS 60.0%
!! CCCTTTCCCTTGGTGTTGGG+AGG + chr2.1:60031009-60031028 MS.gene00059:intron 60.0%
!! TCTATAGCACCGGCACGTGC+CGG - chr2.1:60030449-60030468 None:intergenic 60.0%
TTGTGGACACCGGCACGTGC+CGG + chr2.1:60030437-60030456 MS.gene00059:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 60030180 60033059 60030180 ID=MS.gene00059
chr2.1 mRNA 60030180 60033059 60030180 ID=MS.gene00059.t1;Parent=MS.gene00059
chr2.1 exon 60030180 60030302 60030180 ID=MS.gene00059.t1.exon1;Parent=MS.gene00059.t1
chr2.1 CDS 60030180 60030302 60030180 ID=cds.MS.gene00059.t1;Parent=MS.gene00059.t1
chr2.1 exon 60030507 60030659 60030507 ID=MS.gene00059.t1.exon2;Parent=MS.gene00059.t1
chr2.1 CDS 60030507 60030659 60030507 ID=cds.MS.gene00059.t1;Parent=MS.gene00059.t1
chr2.1 exon 60031751 60031844 60031751 ID=MS.gene00059.t1.exon3;Parent=MS.gene00059.t1
chr2.1 CDS 60031751 60031844 60031751 ID=cds.MS.gene00059.t1;Parent=MS.gene00059.t1
chr2.1 exon 60032105 60032167 60032105 ID=MS.gene00059.t1.exon4;Parent=MS.gene00059.t1
chr2.1 CDS 60032105 60032167 60032105 ID=cds.MS.gene00059.t1;Parent=MS.gene00059.t1
chr2.1 exon 60032371 60032511 60032371 ID=MS.gene00059.t1.exon5;Parent=MS.gene00059.t1
chr2.1 CDS 60032371 60032511 60032371 ID=cds.MS.gene00059.t1;Parent=MS.gene00059.t1
chr2.1 exon 60032824 60033059 60032824 ID=MS.gene00059.t1.exon6;Parent=MS.gene00059.t1
chr2.1 CDS 60032824 60033059 60032824 ID=cds.MS.gene00059.t1;Parent=MS.gene00059.t1
Gene Sequence

>MS.gene00059

ATGGCTTCACGTTCCCCTCGCCTTCTATCACTAGCCAGAAGAGCCTTCTCCTCATCATCTTCTTCTCAAGCTAACGGTGTTTCCTTAGTTCAAGGAGCTTCCCGGGGAATCGGTCTTGAATTCGTAAGATTTTTTCTCCCTCAAAACCAATGTTTGGCATTTCTCACATTGCTAAAAAATTTACTTTTCTTCATGGGGTGTCATTTTATTTTTAGTCTAGGAAGCACTGACACAGACACGAACATTGGACATAATTGTTGTGGACACCGGCACGTGCCGGTGCTATAGAGTTTTTAGTTATTGTGTTGTGTTTTGTATTGTATATAGGTGAAACAATTGTTAGAGAACAATGATAAAGAGCATGTTGTTGCTACTTGCCGTAATCCTAATGCATCAACTGGGCTTCTTCATTTGAAGGATAGATTTGATGATCGACTTCAAATTTTGCCTTTGGATTTGACCGTTGAAAGTTCTATTGAGGTTATGCTTCTTTCTTTCTCATTGTTACATAAAATATGTGTGAATTTAGACACCAAGTTGAGATTTGGTGACCCTTTTAGTTACAAGACTAGAAGGTAGATGCTAAAGTTAATATGGATATGGATAGATATGAGATTTGTGACAAGGTTTAAGAAATATTTTTAGCTAATATTTAACTTTAGGTTTGAATATGCTATTAGTACTTGGGAATATGTCTCCTTCTATGTGGTTAGACTCAAGTGGTTAATGAGCTTCAGAACAAATTGTTCGTAGAACACAAGTTTATATCCTCGAACAATCTTTGGTTGGACTTTACTTATCTCACAGACGAATTCCAGATTACTACAACCCCTTTCCCTTGGTGTTGGGAGGTTAAGATGAAAAACAAAATATGTCTCATTTTGGATTTTGTACTTGCAAAAATCTTTTTTTCATTTGATTTTTTGCTATGCTTGAGTAACACTTCAACAATGCTACATTGGATAAGTGAGGCTGTGTATTCTCCAATTAGTAGCTGCCATATGAAATCTTGCCACATTAGAATAAAATCTTGCCTCACTATAACCATTGCCATTCTTAGTTGTATCAGAAATTCAGCATTCAGCAGGCTTTATCAGTATCCACCCAATACCTTTTATCTTTTACATAAACTAATGCTATGTCATCAAAATGGGCGAACGATTTCGTCTACAAGCCTTGAGCATAAAGGATATTGCACATATCCACCCTTATTTACTATGCTGAGCAGTGATGTCAATCGTATATCGCTGAAAATAACAATTTGTTCACTATGCTACGGTGCTATAGTGCTGCTATAATCACTACTTGACAACACATCGTACCAAATAGCATATCGTAGAACAATAGCTATTTGTTCAAATTCGCTATAGTGCTACAGCTGCTATTTGACAACATTGACCCTGATTAAGTTAACCCACTTGTCAGTTCTGAAATGGGAACTTTTGTCTCAGGAGGATTAACAAATGTAGTGGCCTTTTCTATCAAGACTGATGTTAGACATCTCATGGTTAGATTTAAAAAATCCAAAAACCTATTCATTTGTTAAGCTTTGTGTATTTATAATATTTCAGGCATCTGCATTGTCAATAAAAGAGACATATGGCCATCTAAACCTTCTCATTAATGCATCTGGGATTCTTTCAATACCTGAAGTATTGCAACCAGGTAAAAATTTGTTTCTTTCTTTGGTAATGGACATAATAGAATATCCATCACCTAAGTTTTTCTTCATGTATTCCATGCCCCTTGCATGCACTAAAAACGGAACAATCAAAAGGATTAAACAAGATAGAAAGAAACGGAACATAAATTTGTTGCAGATTTGCGAGAAAAAGGAAAAAAAGTAAGAGTGTTCAACTGTATAGATGGGAGTGTGTAATGGGGGCTAGTGATGTATAACTGTGAGCTAAAACTATTTTATACAGAAACCACGTTGAACAAATTGGAGAAGTCGTCTTTAATGCTTGCATGTGAGGTTAATGCCGTCGGTCCTATCTTGGTTATCAAGGTAACAGCAATCTGAATTTCATTACAGTTTTTTTGTTTGTTTATCTATTTATGATAATTTTAGAAGTAAAATTGGGTTCTTTATGCGTATGAGATTATAGAAGCCTTAGGGCATGTAGTTTGAGTCAAAATTTTGTATCTAAGTCTGTGAATTTCTAAATCAGCACATGTGGCCTCTTCTTAGTTCTTAAAGCTGGAGGTGGCATTGGGACGGAAAGAAGTGGTGCAGTTGTGGCTAGTTTGAGTGCAAGGGTTGCATCTATTGGCGATAATCGTCTCGGGGGTTGGCATTCATATCGGTCTTCAAAGACTGCTCTTAATCAATGTATGACTCTTTTTTGTTGATCACTGTCCTGTTATATTCAATATGATAAGTGCATCATTGGATCATGAATTAGTTTATTTGAGCTAATCTACTAGCAGAAGCACTTATAGAATTATTTGGGAGAACCTATGGAAATAGCTTATAACATGTTCATAAACTGTTTTCAGCTTATTTTCATAAGCTATTTATAAAAATAACTTATAGATTATATGGCATCAATTTGACTTCATTTTATCTTTTGTTAAACGATGATATGATAAACTGTTTGAAATGGTAATCATTAAAAGCTTTTTTCCGCTTATATTCAAACAGTGTCAAAGACGATCTCGTTGGAGTTTGCGAGGAAGAAGGATCCAATCATATGTATTCTATTGCATCCTGGTACAGTTGACACAGATCTTTCTAAGCCATTTCAAAAAAATATTGCTAAAGAAAAGCTCTTCACCAAAGAGTACTCAGTCCAAAAGTTGTTGCACATCATTAACAATGTCAAGAGCCATGACAATGGCAAGTTCTTTGCTTGGGATGGTCAAGAAATTCCTTGGTAA
Protein sequence

>MS.gene00059.t1

MASRSPRLLSLARRAFSSSSSSQANGVSLVQGASRGIGLEFVKQLLENNDKEHVVATCRNPNASTGLLHLKDRFDDRLQILPLDLTVESSIEASALSIKETYGHLNLLINASGILSIPEVLQPETTLNKLEKSSLMLACEVNAVVLKAGGGIGTERSGAVVASLSARVASIGDNRLGGWHSYRSSKTALNQLSKTISLEFARKKDPIICILLHPGTVDTDLSKPFQKNIAKEKLFTKEYSVQKLLHIINNVKSHDNGKFFAWDGQEIPW