Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000681.t1 | XP_003620963.1 | 99.4 | 361 | 2 | 0 | 1 | 361 | 1 | 361 | 7.30E-199 | 703 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000681.t1 | P52904 | 92.0 | 361 | 27 | 2 | 1 | 361 | 1 | 359 | 1.1e-182 | 640.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000681.t1 | B7FJJ4 | 99.4 | 361 | 2 | 0 | 1 | 361 | 1 | 361 | 5.3e-199 | 703.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene011262 | MS.gene000681 | PPI |
| MS.gene037762 | MS.gene000681 | PPI |
| MS.gene052712 | MS.gene000681 | PPI |
| MS.gene000681 | MS.gene049277 | PPI |
| MS.gene000681 | MS.gene06396 | PPI |
| MS.gene000681 | MS.gene073516 | PPI |
| MS.gene80759 | MS.gene000681 | PPI |
| MS.gene000681 | MS.gene054963 | PPI |
| MS.gene000681 | MS.gene81790 | PPI |
| MS.gene000681 | MS.gene53350 | PPI |
| MS.gene000681 | MS.gene013304 | PPI |
| MS.gene000681 | MS.gene07846 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000681.t1 | MTR_7g005380 | 99.446 | 361 | 2 | 0 | 1 | 361 | 1 | 361 | 0.0 | 736 |
| MS.gene000681.t1 | MTR_3g076630 | 41.104 | 326 | 188 | 1 | 27 | 352 | 82 | 403 | 6.60e-88 | 271 |
| MS.gene000681.t1 | MTR_4g021470 | 36.086 | 327 | 202 | 4 | 23 | 348 | 33 | 353 | 3.26e-68 | 218 |
| MS.gene000681.t1 | MTR_7g009250 | 75.000 | 100 | 16 | 2 | 158 | 248 | 16 | 115 | 4.29e-41 | 140 |
| MS.gene000681.t1 | MTR_7g009250 | 58.182 | 55 | 23 | 0 | 85 | 139 | 7 | 61 | 9.46e-12 | 62.0 |
| MS.gene000681.t1 | MTR_7g009290 | 66.667 | 69 | 20 | 1 | 132 | 200 | 9 | 74 | 8.15e-23 | 91.7 |
| MS.gene000681.t1 | MTR_7g009310 | 95.122 | 41 | 2 | 0 | 160 | 200 | 6 | 46 | 1.82e-22 | 90.5 |
| MS.gene000681.t1 | MTR_3g107740 | 27.468 | 233 | 142 | 8 | 66 | 290 | 421 | 634 | 6.30e-11 | 64.3 |
| MS.gene000681.t1 | MTR_8g068265 | 26.587 | 252 | 167 | 8 | 69 | 317 | 427 | 663 | 7.41e-11 | 63.9 |
| MS.gene000681.t1 | MTR_8g068300 | 26.587 | 252 | 167 | 8 | 69 | 317 | 427 | 663 | 8.77e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000681.t1 | AT5G50850 | 85.912 | 362 | 43 | 2 | 1 | 354 | 1 | 362 | 0.0 | 637 |
| MS.gene000681.t1 | AT1G30120 | 40.456 | 351 | 202 | 2 | 5 | 352 | 60 | 406 | 4.08e-90 | 276 |
| MS.gene000681.t1 | AT2G34590 | 42.042 | 333 | 185 | 2 | 22 | 352 | 80 | 406 | 1.72e-89 | 275 |
| MS.gene000681.t1 | AT1G55510 | 36.337 | 344 | 210 | 5 | 8 | 348 | 10 | 347 | 1.28e-69 | 222 |
| MS.gene000681.t1 | AT3G13450 | 35.224 | 335 | 210 | 4 | 15 | 348 | 25 | 353 | 3.17e-68 | 219 |
| MS.gene000681.t1 | AT4G15560 | 24.364 | 275 | 188 | 7 | 19 | 290 | 390 | 647 | 1.02e-11 | 66.6 |
Find 96 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACAAAGGTTATTAATTTA+AGG | 0.162693 | 7.3:+82222652 | MS.gene000681:intron |
| AATATGCAGGCTGGGTTTAC+TGG | 0.232671 | 7.3:+82219781 | MS.gene000681:intron |
| ATGCCCTGGGTTGAAAGTTT+TGG | 0.247026 | 7.3:+82221802 | MS.gene000681:CDS |
| GTTTACTTAAAGCTGCTATA+AGG | 0.289090 | 7.3:+82221852 | MS.gene000681:CDS |
| TCTTGGTATGGCTCATGCCC+TGG | 0.309803 | 7.3:+82221788 | MS.gene000681:CDS |
| TCACCCATCAAGAAGACTTT+AGG | 0.312229 | 7.3:-82210858 | None:intergenic |
| TTAGATTTCCAAGGGACTAT+TGG | 0.314457 | 7.3:+82212817 | MS.gene000681:intron |
| GGAGTTGGTGCTGCTTACTA+TGG | 0.330288 | 7.3:+82219808 | MS.gene000681:CDS |
| CATGCCTTATGCAGCAAATC+TGG | 0.332687 | 7.3:+82222857 | MS.gene000681:CDS |
| TCAAGAACTTCGGCAGAAAC+AGG | 0.334442 | 7.3:-82222040 | None:intergenic |
| AATGGTTGGCTTTGCTCTAA+AGG | 0.349744 | 7.3:+82222247 | MS.gene000681:CDS |
| TCAAGGAAAACTACAGGATC+AGG | 0.356678 | 7.3:-82221878 | None:intergenic |
| CTTTCCAGATTTGCTGCATA+AGG | 0.357769 | 7.3:-82222861 | None:intergenic |
| CAGGCTGGGTTTACTGGGAT+TGG | 0.358479 | 7.3:+82219787 | MS.gene000681:intron |
| TCCGATGGGAAGGCAGAAAC+TGG | 0.372273 | 7.3:-82222065 | None:intergenic |
| TCTCTCTTCTTCCGCTAAAC+AGG | 0.373627 | 7.3:+82210699 | MS.gene000681:CDS |
| AATGTACCTATTGTATTTAG+AGG | 0.374366 | 7.3:+82221564 | MS.gene000681:CDS |
| GCACCGGTGGAGAGAATTGC+CGG | 0.381050 | 7.3:+82222822 | MS.gene000681:CDS |
| GGGTTTACTGGGATTGGAGT+TGG | 0.388394 | 7.3:+82219793 | MS.gene000681:CDS |
| CGAGCATCTTCAGATGAATA+TGG | 0.394225 | 7.3:-82221827 | None:intergenic |
| AATCAAGGAATCAAGATGTT+AGG | 0.403638 | 7.3:+82210538 | None:intergenic |
| TGCAGCAAATCTGGAAAGAT+TGG | 0.409248 | 7.3:+82222866 | MS.gene000681:CDS |
| TTATGCAGTGTTATGCATCT+TGG | 0.410920 | 7.3:+82221771 | MS.gene000681:intron |
| AATGGTGCTGCTGCTGGTGT+TGG | 0.415359 | 7.3:+82221591 | MS.gene000681:CDS |
| TCTGTCATCGAGGAGAGTTT+TGG | 0.424908 | 7.3:+82222789 | MS.gene000681:CDS |
| AAATCAAATTACATGTCTGC+TGG | 0.434500 | 7.3:+82221534 | MS.gene000681:CDS |
| TTTACTTAAAGCTGCTATAA+GGG | 0.435542 | 7.3:+82221853 | MS.gene000681:CDS |
| TTTCCTGACAGAAGCATTGA+TGG | 0.452438 | 7.3:-82222700 | None:intergenic |
| TTGGGTCCTCTAAATACAAT+AGG | 0.453035 | 7.3:-82221570 | None:intergenic |
| GGACCCAATGGTGCTGCTGC+TGG | 0.458395 | 7.3:+82221585 | MS.gene000681:CDS |
| TTCAACTGTCACCAGTCTGT+TGG | 0.461416 | 7.3:-82222724 | None:intergenic |
| CTTGGTATGGCTCATGCCCT+GGG | 0.468165 | 7.3:+82221789 | MS.gene000681:CDS |
| AATGTCATAAACTCCACAAC+AGG | 0.479865 | 7.3:-82219838 | None:intergenic |
| AAGGGACTATTGGAGAAGTA+TGG | 0.485928 | 7.3:+82212827 | MS.gene000681:CDS |
| ATATGCAGGCTGGGTTTACT+GGG | 0.487916 | 7.3:+82219782 | MS.gene000681:intron |
| CAGATTTGCTGCATAAGGCA+TGG | 0.501282 | 7.3:-82222856 | None:intergenic |
| GAGATGTCTGAATGCGGAAA+AGG | 0.501518 | 7.3:-82210680 | None:intergenic |
| GACATTTAACTTCTCCATGC+AGG | 0.502562 | 7.3:+82219855 | MS.gene000681:CDS |
| CAGTGTTATGCATCTTGGTA+TGG | 0.510583 | 7.3:+82221776 | MS.gene000681:intron |
| ACACCAGCAGCAGCACCATT+GGG | 0.513248 | 7.3:-82221588 | None:intergenic |
| GCCCCGGCAATTCTCTCCAC+CGG | 0.513647 | 7.3:-82222825 | None:intergenic |
| ATGCCACAGATCTGTGCCTA+TGG | 0.514735 | 7.3:+82223022 | MS.gene000681:CDS |
| TCACCATCAATGCTTCTGTC+AGG | 0.519872 | 7.3:+82222697 | MS.gene000681:CDS |
| AGATCCTAAAGTCTTCTTGA+TGG | 0.520291 | 7.3:+82210854 | MS.gene000681:CDS |
| ATTTAGGCAGTTGCAGCCAT+AGG | 0.522619 | 7.3:-82223038 | None:intergenic |
| CACCGGTGGAGAGAATTGCC+GGG | 0.523558 | 7.3:+82222823 | MS.gene000681:CDS |
| TTATTAATTTAAGGTCAATC+CGG | 0.524703 | 7.3:+82222661 | MS.gene000681:CDS |
| AACACCAGCAGCAGCACCAT+TGG | 0.529263 | 7.3:-82221589 | None:intergenic |
| TCCTTCAATTTAGATTTCCA+AGG | 0.535088 | 7.3:+82212808 | MS.gene000681:intron |
| GGAGTGTCGAGAACCCTCTC+AGG | 0.535813 | 7.3:-82212851 | None:intergenic |
| AGATTTGCTGCATAAGGCAT+GGG | 0.544500 | 7.3:-82222855 | None:intergenic |
| ATACTTCTCCAATAGTCCCT+TGG | 0.545557 | 7.3:-82212825 | None:intergenic |
| TCCAGTTTCTGCCTTCCCAT+CGG | 0.546005 | 7.3:+82222064 | MS.gene000681:CDS |
| GGGTTTCCTCAGCATGGTGT+TGG | 0.548315 | 7.3:+82222750 | MS.gene000681:CDS |
| TTATAGGTTGGAGAATATCA+AGG | 0.548317 | 7.3:+82212682 | MS.gene000681:intron |
| AGAATATCAAGGTGCATACA+AGG | 0.548665 | 7.3:+82212693 | MS.gene000681:CDS |
| GGAAAGATTGGCTGTCCCAC+AGG | 0.549518 | 7.3:+82222878 | MS.gene000681:CDS |
| GATCCTAAAGTCTTCTTGAT+GGG | 0.550384 | 7.3:+82210855 | MS.gene000681:CDS |
| CAACAAACAAACCTGTTTAG+CGG | 0.555702 | 7.3:-82210710 | None:intergenic |
| AGACTGGTGACAGTTGAAGA+AGG | 0.558832 | 7.3:+82222729 | MS.gene000681:CDS |
| CTTAACAGATTGAGAGAGAA+GGG | 0.559693 | 7.3:+82222195 | MS.gene000681:intron |
| CCTTCAATTTAGATTTCCAA+GGG | 0.567285 | 7.3:+82212809 | MS.gene000681:intron |
| AATCAAATTACATGTCTGCT+GGG | 0.569255 | 7.3:+82221535 | MS.gene000681:CDS |
| AGTCTTCTTGATGGGTGAAG+AGG | 0.572562 | 7.3:+82210863 | MS.gene000681:CDS |
| CTATGGTCTAAAGCCTGTTG+TGG | 0.572678 | 7.3:+82219825 | MS.gene000681:CDS |
| GTTAGGAGTTATAAGAAACA+AGG | 0.577597 | 7.3:+82210555 | MS.gene000681:CDS |
| TGGTGACTCGATCAAGCGGC+CGG | 0.580630 | 7.3:-82222680 | None:intergenic |
| CAAGAACTTCGGCAGAAACA+GGG | 0.590845 | 7.3:-82222039 | None:intergenic |
| GAAACTGGAATCAAGAACTT+CGG | 0.593707 | 7.3:-82222050 | None:intergenic |
| GTGCCAAAACTTTCAACCCA+GGG | 0.603446 | 7.3:-82221805 | None:intergenic |
| CAAGGAAAACTACAGGATCA+GGG | 0.604349 | 7.3:-82221877 | None:intergenic |
| GACTGGTGACAGTTGAAGAA+GGG | 0.606152 | 7.3:+82222730 | MS.gene000681:CDS |
| GAGATAAACACTAACCTGCA+TGG | 0.607390 | 7.3:-82219869 | None:intergenic |
| GCTTAACAGATTGAGAGAGA+AGG | 0.608904 | 7.3:+82222194 | MS.gene000681:intron |
| TGTCAGGAAAACCAACAGAC+TGG | 0.614276 | 7.3:+82222713 | MS.gene000681:CDS |
| CAGCCATAGGCACAGATCTG+TGG | 0.618473 | 7.3:-82223025 | None:intergenic |
| AGGCATGGGAACATCAGCCC+CGG | 0.619316 | 7.3:-82222841 | None:intergenic |
| GGAGAAGTATGGTCCTGAGA+GGG | 0.619724 | 7.3:+82212838 | MS.gene000681:CDS |
| ATTGTATTTAGAGGACCCAA+TGG | 0.620504 | 7.3:+82221573 | MS.gene000681:CDS |
| GAAGAAGGGTTTCCTCAGCA+TGG | 0.620750 | 7.3:+82222744 | MS.gene000681:CDS |
| GGTGCCAAAACTTTCAACCC+AGG | 0.622618 | 7.3:-82221806 | None:intergenic |
| CCTTCCCATCGGAAAAGCAA+AGG | 0.627357 | 7.3:+82222075 | MS.gene000681:CDS |
| GCTGAGACACTAGCAAAGGA+AGG | 0.630656 | 7.3:+82222530 | MS.gene000681:CDS |
| GGCTGCTGAGACACTAGCAA+AGG | 0.630698 | 7.3:+82222526 | MS.gene000681:intron |
| CAAGAAGACTTTAGGATCTG+CGG | 0.636868 | 7.3:-82210850 | None:intergenic |
| TGGCTATCTTGATGCACCGG+TGG | 0.641507 | 7.3:+82222809 | MS.gene000681:CDS |
| ACCGGTGGAGAGAATTGCCG+GGG | 0.643645 | 7.3:+82222824 | MS.gene000681:CDS |
| TTGATGGTGACTCGATCAAG+CGG | 0.653569 | 7.3:-82222684 | None:intergenic |
| TATTCATCTGAAGATGCTCG+TGG | 0.654209 | 7.3:+82221830 | MS.gene000681:CDS |
| AAAGGAAGGAATCAGTGCTG+AGG | 0.656553 | 7.3:+82222544 | MS.gene000681:CDS |
| TGGAGAAGTATGGTCCTGAG+AGG | 0.669482 | 7.3:+82212837 | MS.gene000681:CDS |
| TGTCTGAATGCGGAAAAGGA+AGG | 0.671745 | 7.3:-82210676 | None:intergenic |
| TCAGCGCCAACACCATGCTG+AGG | 0.686283 | 7.3:-82222756 | None:intergenic |
| AGAAGAGAGATGTCTGAATG+CGG | 0.692625 | 7.3:-82210686 | None:intergenic |
| AATCTGTGCATCTGTCATCG+AGG | 0.746298 | 7.3:+82222779 | MS.gene000681:CDS |
| TCTCGACACTCCCATCACTG+AGG | 0.757157 | 7.3:+82212862 | MS.gene000681:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 82210553 | 82223058 | 82210553 | ID=MS.gene000681 |
| chr7.3 | mRNA | 82210553 | 82223058 | 82210553 | ID=MS.gene000681.t1;Parent=MS.gene000681 |
| chr7.3 | exon | 82210553 | 82210576 | 82210553 | ID=MS.gene000681.t1.exon1;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82210553 | 82210576 | 82210553 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82210664 | 82210720 | 82210664 | ID=MS.gene000681.t1.exon2;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82210664 | 82210720 | 82210664 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82210804 | 82210884 | 82210804 | ID=MS.gene000681.t1.exon3;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82210804 | 82210884 | 82210804 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82212688 | 82212714 | 82212688 | ID=MS.gene000681.t1.exon4;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82212688 | 82212714 | 82212688 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82212821 | 82212883 | 82212821 | ID=MS.gene000681.t1.exon5;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82212821 | 82212883 | 82212821 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82219790 | 82219876 | 82219790 | ID=MS.gene000681.t1.exon6;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82219790 | 82219876 | 82219790 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82221504 | 82221629 | 82221504 | ID=MS.gene000681.t1.exon7;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82221504 | 82221629 | 82221504 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82221779 | 82221912 | 82221779 | ID=MS.gene000681.t1.exon8;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82221779 | 82221912 | 82221779 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82222024 | 82222096 | 82222024 | ID=MS.gene000681.t1.exon9;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82222024 | 82222096 | 82222024 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82222203 | 82222268 | 82222203 | ID=MS.gene000681.t1.exon10;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82222203 | 82222268 | 82222203 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82222527 | 82222565 | 82222527 | ID=MS.gene000681.t1.exon11;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82222527 | 82222565 | 82222527 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82222660 | 82222899 | 82222660 | ID=MS.gene000681.t1.exon12;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82222660 | 82222899 | 82222660 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| chr7.3 | exon | 82222990 | 82223058 | 82222990 | ID=MS.gene000681.t1.exon13;Parent=MS.gene000681.t1 |
| chr7.3 | CDS | 82222990 | 82223058 | 82222990 | ID=cds.MS.gene000681.t1;Parent=MS.gene000681.t1 |
| Gene Sequence |
| Protein sequence |
