AlfalfaGEDB | MS.gene002382

M. sativa cultivar XinJiangDaYe / MS.gene002382

KEGG: K02898
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00467.32 PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002382.t1	XP_003593461.1	99.3	146	1	0	1	146	1	146	2.30E-71	278.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002382.t1	Q9FJX2	87.7	146	18	0	1	146	1	146	9.7e-66	250.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002382.t1	G7ILF2	99.3	146	1	0	1	146	1	146	1.7e-71	278.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene01768	MS.gene002382	PPI
MS.gene01360	MS.gene002382	PPI
MS.gene43544	MS.gene002382	PPI
MS.gene20295	MS.gene002382	PPI
MS.gene002382	MS.gene09597	PPI
MS.gene047519	MS.gene002382	PPI
MS.gene015255	MS.gene002382	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002382.t1	MTR_2g012450	99.315	146	1	0	1	146	1	146	2.48e-103	291
MS.gene002382.t1	MTR_5g015570	94.521	146	8	0	1	146	1	146	5.12e-90	258
MS.gene002382.t1	MTR_5g096540	91.781	146	12	0	1	146	1	146	2.07e-87	251
MS.gene002382.t1	MTR_4g126220	86.765	68	9	0	13	80	103	170	1.52e-37	126
MS.gene002382.t1	MTR_8g073140	56.977	86	10	1	30	115	1	59	2.78e-23	87.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002382.t1	AT5G67510	87.671	146	18	0	1	146	1	146	2.18e-92	264
MS.gene002382.t1	AT3G49910	86.301	146	20	0	1	146	1	146	5.24e-92	263

Find 48 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTGGTGTTAATCCTTCTA+AGG	0.281571	2.4:+71809468	MS.gene002382:CDS
TCGCAAAGACGACGAAGTTC+AGG	0.349938	2.4:+71809330	MS.gene002382:CDS
TATTATGCAGACCGTTGATT+AGG	0.363868	2.4:+71809603	MS.gene002382:CDS
CATTGAACGGACGTTGTACT+TGG	0.377101	2.4:-71809302	None:intergenic
TTAGTGATAACAACCTTAGA+AGG	0.401319	2.4:-71809481	None:intergenic
ACTTCGTCGTCTTTGCGAAC+AGG	0.416424	2.4:-71809325	None:intergenic
GGGTCGTGCTGCTGCTGATA+AGG	0.420559	2.4:+71809552	MS.gene002382:CDS
ACGGCTCTTCCTACGGCTGC+TGG	0.422834	2.4:-71809206	None:intergenic
ATGACACGGCGGACGCTTGA+TGG	0.428692	2.4:-71809247	None:intergenic
CTTGAGTAACCTTACCTTCA+CGG	0.428858	2.4:-71809375	None:intergenic
CACTAAGCTCAGATTGGATA+AGG	0.438891	2.4:+71809498	MS.gene002382:CDS
TGTTATCACTAAGCTCAGAT+TGG	0.466490	2.4:+71809492	MS.gene002382:CDS
AGGATTAACACCAACATTAA+CGG	0.467585	2.4:-71809461	None:intergenic
CTCAAGTTTACCGACGCAAG+TGG	0.488086	2.4:+71809392	MS.gene002382:CDS
ATTCAAAGGCCGTGAAGGTA+AGG	0.498862	2.4:+71809366	MS.gene002382:CDS
GACGAAGTTCAGGTTGTTCG+TGG	0.501347	2.4:+71809340	MS.gene002382:CDS
GCTTCTTGATCGTAAGGCTA+AGG	0.520683	2.4:+71809531	MS.gene002382:CDS
CTACGGCTGCTGGAAACTCG+TGG	0.530535	2.4:-71809196	None:intergenic
TGCTGCTGCTGATAAGGAGA+AGG	0.537077	2.4:+71809558	MS.gene002382:CDS
GAGCCGTAAGGCTCATTTCA+CGG	0.545137	2.4:+71809222	MS.gene002382:CDS
ATCACAAGGGAGAAGGTGAA+TGG	0.545267	2.4:+71809433	MS.gene002382:CDS
GAGCCTTACGGCTCTTCCTA+CGG	0.547173	2.4:-71809213	None:intergenic
CTTCTTGATCGTAAGGCTAA+GGG	0.547721	2.4:+71809532	MS.gene002382:CDS
TAAGTCGCTTCTTGATCGTA+AGG	0.557796	2.4:+71809525	MS.gene002382:CDS
GTTGTTCGTGGAACATTCAA+AGG	0.558204	2.4:+71809352	MS.gene002382:CDS
GGGTACTAAGTTTGCTCCTG+AGG	0.563912	2.4:+71809579	MS.gene002382:CDS
AACGGACGTTGTACTTGGAT+CGG	0.564652	2.4:-71809297	None:intergenic
CTTTGCGAACAGGCATTGAA+CGG	0.570326	2.4:-71809315	None:intergenic
GTTGTACTTGGATCGGAGAT+CGG	0.572814	2.4:-71809290	None:intergenic
ACGGTCTGCATAATATCCTC+AGG	0.575139	2.4:-71809595	None:intergenic
GCGCCGTGAAATGAGCCTTA+CGG	0.575259	2.4:-71809225	None:intergenic
CGAACGCATCACAAGGGAGA+AGG	0.580140	2.4:+71809426	MS.gene002382:CDS
GATCGGAGATCGGCGGAGAG+TGG	0.584970	2.4:-71809280	None:intergenic
TACGGCTGCTGGAAACTCGT+GGG	0.586325	2.4:-71809195	None:intergenic
TTCACATCGAACGCATCACA+AGG	0.588472	2.4:+71809419	MS.gene002382:CDS
CAGCCGTAGGAAGAGCCGTA+AGG	0.591177	2.4:+71809210	MS.gene002382:CDS
GGAACATTCAAAGGCCGTGA+AGG	0.606062	2.4:+71809361	MS.gene002382:CDS
GATTCAAGAATCCTAATCAA+CGG	0.625900	2.4:-71809614	None:intergenic
AATGGATCCACCGTTAATGT+TGG	0.632764	2.4:+71809451	MS.gene002382:CDS
GCTGCTGCTGATAAGGAGAA+GGG	0.634378	2.4:+71809559	MS.gene002382:CDS
TCAAGTTTACCGACGCAAGT+GGG	0.644269	2.4:+71809393	MS.gene002382:CDS
ATTAACACCAACATTAACGG+TGG	0.662702	2.4:-71809458	None:intergenic
TGTGAATCACCCACTTGCGT+CGG	0.677070	2.4:-71809402	None:intergenic
CACGAGTTTCCAGCAGCCGT+AGG	0.686122	2.4:+71809197	MS.gene002382:CDS
GTACTTGGATCGGAGATCGG+CGG	0.709980	2.4:-71809287	None:intergenic
TCACATCGAACGCATCACAA+GGG	0.710980	2.4:+71809420	MS.gene002382:CDS
GTGGCGCGCTCATTATGACA+CGG	0.719503	2.4:-71809261	None:intergenic
GCGCGCTCATTATGACACGG+CGG	0.837709	2.4:-71809258	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGGATTAACACCAACATTAA+CGG	-	chr2.4:71809464-71809483	None:intergenic	30.0%
	TTAGTGATAACAACCTTAGA+AGG	-	chr2.4:71809484-71809503	None:intergenic	30.0%
	ATTAACACCAACATTAACGG+TGG	-	chr2.4:71809461-71809480	None:intergenic	35.0%
	TGTTATCACTAAGCTCAGAT+TGG	+	chr2.4:71809492-71809511	MS.gene002382:CDS	35.0%
!!	TGTTGGTGTTAATCCTTCTA+AGG	+	chr2.4:71809468-71809487	MS.gene002382:CDS	35.0%
	AATGGATCCACCGTTAATGT+TGG	+	chr2.4:71809451-71809470	MS.gene002382:CDS	40.0%
	CACTAAGCTCAGATTGGATA+AGG	+	chr2.4:71809498-71809517	MS.gene002382:CDS	40.0%
	CTTCTTGATCGTAAGGCTAA+GGG	+	chr2.4:71809532-71809551	MS.gene002382:CDS	40.0%
	CTTGAGTAACCTTACCTTCA+CGG	-	chr2.4:71809378-71809397	None:intergenic	40.0%
	GTTGTTCGTGGAACATTCAA+AGG	+	chr2.4:71809352-71809371	MS.gene002382:CDS	40.0%
	TAAGTCGCTTCTTGATCGTA+AGG	+	chr2.4:71809525-71809544	MS.gene002382:CDS	40.0%
	AACGGACGTTGTACTTGGAT+CGG	-	chr2.4:71809300-71809319	None:intergenic	45.0%
	ACGGTCTGCATAATATCCTC+AGG	-	chr2.4:71809598-71809617	None:intergenic	45.0%
	ATCACAAGGGAGAAGGTGAA+TGG	+	chr2.4:71809433-71809452	MS.gene002382:CDS	45.0%
	ATTCAAAGGCCGTGAAGGTA+AGG	+	chr2.4:71809366-71809385	MS.gene002382:CDS	45.0%
	CATTGAACGGACGTTGTACT+TGG	-	chr2.4:71809305-71809324	None:intergenic	45.0%
	GCTTCTTGATCGTAAGGCTA+AGG	+	chr2.4:71809531-71809550	MS.gene002382:CDS	45.0%
	GTTGTACTTGGATCGGAGAT+CGG	-	chr2.4:71809293-71809312	None:intergenic	45.0%
	TCAAGTTTACCGACGCAAGT+GGG	+	chr2.4:71809393-71809412	MS.gene002382:CDS	45.0%
	TCACATCGAACGCATCACAA+GGG	+	chr2.4:71809420-71809439	MS.gene002382:CDS	45.0%
	TTCACATCGAACGCATCACA+AGG	+	chr2.4:71809419-71809438	MS.gene002382:CDS	45.0%
!!	CTTTGCGAACAGGCATTGAA+CGG	-	chr2.4:71809318-71809337	None:intergenic	45.0%
	ACTTCGTCGTCTTTGCGAAC+AGG	-	chr2.4:71809328-71809347	None:intergenic	50.0%
	CTCAAGTTTACCGACGCAAG+TGG	+	chr2.4:71809392-71809411	MS.gene002382:CDS	50.0%
	GAGCCGTAAGGCTCATTTCA+CGG	+	chr2.4:71809222-71809241	MS.gene002382:CDS	50.0%
	GCTGCTGCTGATAAGGAGAA+GGG	+	chr2.4:71809559-71809578	MS.gene002382:CDS	50.0%
	GGAACATTCAAAGGCCGTGA+AGG	+	chr2.4:71809361-71809380	MS.gene002382:CDS	50.0%
	TCGCAAAGACGACGAAGTTC+AGG	+	chr2.4:71809330-71809349	MS.gene002382:CDS	50.0%
	TGCTGCTGCTGATAAGGAGA+AGG	+	chr2.4:71809558-71809577	MS.gene002382:CDS	50.0%
	TGTGAATCACCCACTTGCGT+CGG	-	chr2.4:71809405-71809424	None:intergenic	50.0%
!	GGGTACTAAGTTTGCTCCTG+AGG	+	chr2.4:71809579-71809598	MS.gene002382:CDS	50.0%
!!	GACGAAGTTCAGGTTGTTCG+TGG	+	chr2.4:71809340-71809359	MS.gene002382:CDS	50.0%
	CGAACGCATCACAAGGGAGA+AGG	+	chr2.4:71809426-71809445	MS.gene002382:CDS	55.0%
	GAGCCTTACGGCTCTTCCTA+CGG	-	chr2.4:71809216-71809235	None:intergenic	55.0%
	GCGCCGTGAAATGAGCCTTA+CGG	-	chr2.4:71809228-71809247	None:intergenic	55.0%
	GTACTTGGATCGGAGATCGG+CGG	-	chr2.4:71809290-71809309	None:intergenic	55.0%
	GTGGCGCGCTCATTATGACA+CGG	-	chr2.4:71809264-71809283	None:intergenic	55.0%
	TACGGCTGCTGGAAACTCGT+GGG	-	chr2.4:71809198-71809217	None:intergenic	55.0%
	ATGACACGGCGGACGCTTGA+TGG	-	chr2.4:71809250-71809269	None:intergenic	60.0%
	CACGAGTTTCCAGCAGCCGT+AGG	+	chr2.4:71809197-71809216	MS.gene002382:CDS	60.0%
	CAGCCGTAGGAAGAGCCGTA+AGG	+	chr2.4:71809210-71809229	MS.gene002382:CDS	60.0%
	CTACGGCTGCTGGAAACTCG+TGG	-	chr2.4:71809199-71809218	None:intergenic	60.0%
	GCGCGCTCATTATGACACGG+CGG	-	chr2.4:71809261-71809280	None:intergenic	60.0%
!	GGGTCGTGCTGCTGCTGATA+AGG	+	chr2.4:71809552-71809571	MS.gene002382:CDS	60.0%
	ACGGCTCTTCCTACGGCTGC+TGG	-	chr2.4:71809209-71809228	None:intergenic	65.0%
	GATCGGAGATCGGCGGAGAG+TGG	-	chr2.4:71809283-71809302	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	71809184	71809624	71809184	ID=MS.gene002382
chr2.4	mRNA	71809184	71809624	71809184	ID=MS.gene002382.t1;Parent=MS.gene002382
chr2.4	exon	71809184	71809624	71809184	ID=MS.gene002382.t1.exon1;Parent=MS.gene002382.t1
chr2.4	CDS	71809184	71809624	71809184	ID=cds.MS.gene002382.t1;Parent=MS.gene002382.t1

Gene Sequence

>MS.gene002382

ATGAAGTTCAACCCACGAGTTTCCAGCAGCCGTAGGAAGAGCCGTAAGGCTCATTTCACGGCGCCATCAAGCGTCCGCCGTGTCATAATGAGCGCGCCACTCTCCGCCGATCTCCGATCCAAGTACAACGTCCGTTCAATGCCTGTTCGCAAAGACGACGAAGTTCAGGTTGTTCGTGGAACATTCAAAGGCCGTGAAGGTAAGGTTACTCAAGTTTACCGACGCAAGTGGGTGATTCACATCGAACGCATCACAAGGGAGAAGGTGAATGGATCCACCGTTAATGTTGGTGTTAATCCTTCTAAGGTTGTTATCACTAAGCTCAGATTGGATAAGGATCGTAAGTCGCTTCTTGATCGTAAGGCTAAGGGTCGTGCTGCTGCTGATAAGGAGAAGGGTACTAAGTTTGCTCCTGAGGATATTATGCAGACCGTTGATTAG

Protein sequence

>MS.gene002382.t1

MKFNPRVSSSRRKSRKAHFTAPSSVRRVIMSAPLSADLRSKYNVRSMPVRKDDEVQVVRGTFKGREGKVTQVYRRKWVIHIERITREKVNGSTVNVGVNPSKVVITKLRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQTVD