AlfalfaGEDB | MS.gene20295

M. sativa cultivar XinJiangDaYe / MS.gene20295

KEGG: K02898
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00467.32 PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20295.t1	XP_003611586.2	100	146	0	0	1	146	1	146	3.60E-72	280.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20295.t1	P51414	85.6	146	21	0	1	146	1	146	1.3e-65	250.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20295.t1	G7K2J3	100.0	146	0	0	1	146	1	146	2.6e-72	280.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050252	MS.gene20295	0.819302	1.25E-52	-1.69E-46
MS.gene054733	MS.gene20295	0.824697	7.01E-54	-1.69E-46
MS.gene059340	MS.gene20295	0.859865	3.08E-63	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene20295	MS.gene01360	PPI
MS.gene20295	MS.gene047519	PPI
MS.gene01360	MS.gene20295	PPI
MS.gene20295	MS.gene002382	PPI
MS.gene20295	MS.gene09597	PPI
MS.gene20295	MS.gene43544	PPI
MS.gene20295	MS.gene01768	PPI
MS.gene20295	MS.gene015255	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20295.t1	MTR_5g015570	100.000	146	0	0	1	146	1	146	3.19e-103	291
MS.gene20295.t1	MTR_2g012450	95.205	146	7	0	1	146	1	146	2.71e-90	259
MS.gene20295.t1	MTR_5g096540	91.781	146	12	0	1	146	1	146	8.14e-88	253
MS.gene20295.t1	MTR_4g126220	92.647	68	5	0	13	80	103	170	1.42e-39	131
MS.gene20295.t1	MTR_8g073140	60.465	86	7	1	30	115	1	59	8.93e-25	90.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20295.t1	AT5G67510	86.301	146	20	0	1	146	1	146	2.43e-91	261
MS.gene20295.t1	AT3G49910	85.616	146	21	0	1	146	1	146	2.43e-91	261

Find 48 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCCCTTCACGTCCCTTGA+AGG	0.334674	5.1:-8020949	None:intergenic
TGCAAGTTTATCGTAGGAAA+TGG	0.354784	5.1:+8020976	MS.gene20295:CDS
TACCAAGATTCGTTTGGATA+AGG	0.385625	5.1:+8021082	MS.gene20295:CDS
ATCCTTATCCAAACGAATCT+TGG	0.396970	5.1:-8021084	None:intergenic
AAAACACGACGGACGCTTGA+TGG	0.398070	5.1:-8020831	None:intergenic
ATTACACGTGAGAAGGTTAA+TGG	0.405029	5.1:+8021017	MS.gene20295:CDS
TGTGATTACCAAGATTCGTT+TGG	0.408401	5.1:+8021076	MS.gene20295:CDS
TTGGTAATCACAACCTTTGA+AGG	0.416639	5.1:-8021065	None:intergenic
GGGTCGTGCCGCTGCTGATA+AGG	0.421050	5.1:+8021136	MS.gene20295:CDS
TCGTAAGGATGATGAAGTTC+AGG	0.424559	5.1:+8020914	MS.gene20295:CDS
CGACGGCTGCTTGAAACCCT+AGG	0.429571	5.1:-8020780	None:intergenic
TGCCGCTGCTGATAAGGAAA+AGG	0.460452	5.1:+8021142	MS.gene20295:CDS
AAACACGACGGACGCTTGAT+GGG	0.465655	5.1:-8020830	None:intergenic
GCGTTCTCTTCCCGTTCGTA+AGG	0.475960	5.1:+8020899	MS.gene20295:CDS
AATGTCTTCAGGAGCAAACT+TGG	0.478032	5.1:-8021168	None:intergenic
GAAAAGCTTGCTTGATCGTA+AGG	0.501897	5.1:+8021109	MS.gene20295:CDS
GCAAGTTTATCGTAGGAAAT+GGG	0.504322	5.1:+8020977	MS.gene20295:CDS
ACTTCATCATCCTTACGAAC+GGG	0.506464	5.1:-8020909	None:intergenic
TTGATGAGCGCACCTCTCTC+CGG	0.508962	5.1:+8020852	MS.gene20295:CDS
ACACAATGAAGTTCAACCCT+AGG	0.518183	5.1:+8020763	None:intergenic
GGTAGTGAGAGGAACCTTCA+AGG	0.522192	5.1:+8020935	MS.gene20295:CDS
GTACTTTGAACGGAGGTCAC+CGG	0.530458	5.1:-8020871	None:intergenic
CAGCCGTCGCAAGAGCCGCA+AGG	0.548428	5.1:+8020794	MS.gene20295:CDS
CACAATGAAGTTCAACCCTA+GGG	0.551275	5.1:+8020764	None:intergenic
GCCGCTGCTGATAAGGAAAA+GGG	0.554219	5.1:+8021143	MS.gene20295:CDS
AATGGGTGATTCATATTGAG+AGG	0.559538	5.1:+8020994	MS.gene20295:CDS
AACTTCATCATCCTTACGAA+CGG	0.563073	5.1:-8020910	None:intergenic
GGAACCTTCAAGGGACGTGA+AGG	0.563323	5.1:+8020945	MS.gene20295:CDS
ACGACGGACGCTTGATGGGG+CGG	0.563774	5.1:-8020826	None:intergenic
GACGGCTGCTTGAAACCCTA+GGG	0.565492	5.1:-8020779	None:intergenic
TGTTGGTGTTGATCCTTCAA+AGG	0.568879	5.1:+8021052	MS.gene20295:CDS
GAGCCTTGCGGCTCTTGCGA+CGG	0.572981	5.1:-8020797	None:intergenic
CTTGCTTGATCGTAAGGCGA+AGG	0.575754	5.1:+8021115	MS.gene20295:CDS
AACGCACGTTGTACTTTGAA+CGG	0.578356	5.1:-8020881	None:intergenic
AGATTCGTTTGGATAAGGAT+AGG	0.578817	5.1:+8021087	MS.gene20295:CDS
AACACGACGGACGCTTGATG+GGG	0.578903	5.1:-8020829	None:intergenic
TGAGAGGATTACACGTGAGA+AGG	0.580146	5.1:+8021010	MS.gene20295:CDS
GATGAAGTTCAGGTAGTGAG+AGG	0.591688	5.1:+8020924	MS.gene20295:CDS
CTTCAAGGGACGTGAAGGGA+AGG	0.593141	5.1:+8020950	MS.gene20295:CDS
AGGTTGTGCAAGTTTATCGT+AGG	0.600876	5.1:+8020970	MS.gene20295:CDS
AATGGTCAAACTGTGAATGT+TGG	0.612366	5.1:+8021035	MS.gene20295:CDS
TTGCTTGATCGTAAGGCGAA+GGG	0.621055	5.1:+8021116	MS.gene20295:CDS
GAACGGAGGTCACCGGAGAG+AGG	0.625041	5.1:-8020864	None:intergenic
GTAGTGAGAGGAACCTTCAA+GGG	0.634832	5.1:+8020936	MS.gene20295:CDS
GAACCTTCAAGGGACGTGAA+GGG	0.643186	5.1:+8020946	MS.gene20295:CDS
GCACGTTGTACTTTGAACGG+AGG	0.673729	5.1:-8020878	None:intergenic
GGGCGGTGAAATGAGCCTTG+CGG	0.681827	5.1:-8020809	None:intergenic
GTGCGCTCATCAAAACACGA+CGG	0.772319	5.1:-8020842	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACTTCATCATCCTTACGAA+CGG	-	chr5.1:8020913-8020932	None:intergenic	35.0%
	AATGGGTGATTCATATTGAG+AGG	+	chr5.1:8020994-8021013	MS.gene20295:CDS	35.0%
	AATGGTCAAACTGTGAATGT+TGG	+	chr5.1:8021035-8021054	MS.gene20295:CDS	35.0%
	AGATTCGTTTGGATAAGGAT+AGG	+	chr5.1:8021087-8021106	MS.gene20295:CDS	35.0%
	ATCCTTATCCAAACGAATCT+TGG	-	chr5.1:8021087-8021106	None:intergenic	35.0%
	ATTACACGTGAGAAGGTTAA+TGG	+	chr5.1:8021017-8021036	MS.gene20295:CDS	35.0%
	GCAAGTTTATCGTAGGAAAT+GGG	+	chr5.1:8020977-8020996	MS.gene20295:CDS	35.0%
	TACCAAGATTCGTTTGGATA+AGG	+	chr5.1:8021082-8021101	MS.gene20295:CDS	35.0%
	TGCAAGTTTATCGTAGGAAA+TGG	+	chr5.1:8020976-8020995	MS.gene20295:CDS	35.0%
	TGTGATTACCAAGATTCGTT+TGG	+	chr5.1:8021076-8021095	MS.gene20295:CDS	35.0%
!	ACGTTTTGCATAATGTCTTC+AGG	-	chr5.1:8021182-8021201	None:intergenic	35.0%
!	TTGGTAATCACAACCTTTGA+AGG	-	chr5.1:8021068-8021087	None:intergenic	35.0%
	AACGCACGTTGTACTTTGAA+CGG	-	chr5.1:8020884-8020903	None:intergenic	40.0%
	AATGTCTTCAGGAGCAAACT+TGG	-	chr5.1:8021171-8021190	None:intergenic	40.0%
	ACTTCATCATCCTTACGAAC+GGG	-	chr5.1:8020912-8020931	None:intergenic	40.0%
	AGGTTGTGCAAGTTTATCGT+AGG	+	chr5.1:8020970-8020989	MS.gene20295:CDS	40.0%
	TCGTAAGGATGATGAAGTTC+AGG	+	chr5.1:8020914-8020933	MS.gene20295:CDS	40.0%
!	GAAAAGCTTGCTTGATCGTA+AGG	+	chr5.1:8021109-8021128	MS.gene20295:CDS	40.0%
!!	TGTTGGTGTTGATCCTTCAA+AGG	+	chr5.1:8021052-8021071	MS.gene20295:CDS	40.0%
	GATGAAGTTCAGGTAGTGAG+AGG	+	chr5.1:8020924-8020943	MS.gene20295:CDS	45.0%
	GTAGTGAGAGGAACCTTCAA+GGG	+	chr5.1:8020936-8020955	MS.gene20295:CDS	45.0%
	TGAGAGGATTACACGTGAGA+AGG	+	chr5.1:8021010-8021029	MS.gene20295:CDS	45.0%
	TTGCTTGATCGTAAGGCGAA+GGG	+	chr5.1:8021116-8021135	MS.gene20295:CDS	45.0%
!	ACCCTTTTCCTTATCAGCAG+CGG	-	chr5.1:8021147-8021166	None:intergenic	45.0%
	AAAACACGACGGACGCTTGA+TGG	-	chr5.1:8020834-8020853	None:intergenic	50.0%
	AAACACGACGGACGCTTGAT+GGG	-	chr5.1:8020833-8020852	None:intergenic	50.0%
	CTTGCTTGATCGTAAGGCGA+AGG	+	chr5.1:8021115-8021134	MS.gene20295:CDS	50.0%
	GAACCTTCAAGGGACGTGAA+GGG	+	chr5.1:8020946-8020965	MS.gene20295:CDS	50.0%
	GCACGTTGTACTTTGAACGG+AGG	-	chr5.1:8020881-8020900	None:intergenic	50.0%
	GCCGCTGCTGATAAGGAAAA+GGG	+	chr5.1:8021143-8021162	MS.gene20295:CDS	50.0%
	GGTAGTGAGAGGAACCTTCA+AGG	+	chr5.1:8020935-8020954	MS.gene20295:CDS	50.0%
	GTACTTTGAACGGAGGTCAC+CGG	-	chr5.1:8020874-8020893	None:intergenic	50.0%
	GTGCGCTCATCAAAACACGA+CGG	-	chr5.1:8020845-8020864	None:intergenic	50.0%
	TGCCGCTGCTGATAAGGAAA+AGG	+	chr5.1:8021142-8021161	MS.gene20295:CDS	50.0%
	AACACGACGGACGCTTGATG+GGG	-	chr5.1:8020832-8020851	None:intergenic	55.0%
	CTTCAAGGGACGTGAAGGGA+AGG	+	chr5.1:8020950-8020969	MS.gene20295:CDS	55.0%
	CTTCCCTTCACGTCCCTTGA+AGG	-	chr5.1:8020952-8020971	None:intergenic	55.0%
	GACGGCTGCTTGAAACCCTA+GGG	-	chr5.1:8020782-8020801	None:intergenic	55.0%
	GCGTTCTCTTCCCGTTCGTA+AGG	+	chr5.1:8020899-8020918	MS.gene20295:CDS	55.0%
	GGAACCTTCAAGGGACGTGA+AGG	+	chr5.1:8020945-8020964	MS.gene20295:CDS	55.0%
	TTGATGAGCGCACCTCTCTC+CGG	+	chr5.1:8020852-8020871	MS.gene20295:CDS	55.0%
	CGACGGCTGCTTGAAACCCT+AGG	-	chr5.1:8020783-8020802	None:intergenic	60.0%
	GGGCGGTGAAATGAGCCTTG+CGG	-	chr5.1:8020812-8020831	None:intergenic	60.0%
	ACGACGGACGCTTGATGGGG+CGG	-	chr5.1:8020829-8020848	None:intergenic	65.0%
	GAACGGAGGTCACCGGAGAG+AGG	-	chr5.1:8020867-8020886	None:intergenic	65.0%
	GAGCCTTGCGGCTCTTGCGA+CGG	-	chr5.1:8020800-8020819	None:intergenic	65.0%
!	GGGTCGTGCCGCTGCTGATA+AGG	+	chr5.1:8021136-8021155	MS.gene20295:CDS	65.0%
	CAGCCGTCGCAAGAGCCGCA+AGG	+	chr5.1:8020794-8020813	MS.gene20295:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	8020768	8021208	8020768	ID=MS.gene20295
chr5.1	mRNA	8020768	8021208	8020768	ID=MS.gene20295.t1;Parent=MS.gene20295
chr5.1	exon	8020768	8021208	8020768	ID=MS.gene20295.t1.exon1;Parent=MS.gene20295.t1
chr5.1	CDS	8020768	8021208	8020768	ID=cds.MS.gene20295.t1;Parent=MS.gene20295.t1

Gene Sequence

>MS.gene20295

ATGAAGTTCAACCCTAGGGTTTCAAGCAGCCGTCGCAAGAGCCGCAAGGCTCATTTCACCGCCCCATCAAGCGTCCGTCGTGTTTTGATGAGCGCACCTCTCTCCGGTGACCTCCGTTCAAAGTACAACGTGCGTTCTCTTCCCGTTCGTAAGGATGATGAAGTTCAGGTAGTGAGAGGAACCTTCAAGGGACGTGAAGGGAAGGTTGTGCAAGTTTATCGTAGGAAATGGGTGATTCATATTGAGAGGATTACACGTGAGAAGGTTAATGGTCAAACTGTGAATGTTGGTGTTGATCCTTCAAAGGTTGTGATTACCAAGATTCGTTTGGATAAGGATAGGAAAAGCTTGCTTGATCGTAAGGCGAAGGGTCGTGCCGCTGCTGATAAGGAAAAGGGTACCAAGTTTGCTCCTGAAGACATTATGCAAAACGTTGATTAG

Protein sequence

>MS.gene20295.t1

MKFNPRVSSSRRKSRKAHFTAPSSVRRVLMSAPLSGDLRSKYNVRSLPVRKDDEVQVVRGTFKGREGKVVQVYRRKWVIHIERITREKVNGQTVNVGVDPSKVVITKIRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQNVD