AlfalfaGEDB | MS.gene43544

M. sativa cultivar XinJiangDaYe / MS.gene43544

KEGG: K02898
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00467.32 PF16906.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43544.t1	XP_003611586.2	99.3	146	1	0	1	146	1	146	5.20E-71	276.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43544.t1	P51414	84.9	146	22	0	1	146	1	146	1.8e-64	246.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43544.t1	G7K2J3	99.3	146	1	0	1	146	1	146	3.8e-71	276.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05491	MS.gene43544	0.821873	3.20E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene047519	MS.gene43544	PPI
MS.gene01768	MS.gene43544	PPI
MS.gene43544	MS.gene002382	PPI
MS.gene015255	MS.gene43544	PPI
MS.gene43544	MS.gene09597	PPI
MS.gene01360	MS.gene43544	PPI
MS.gene20295	MS.gene43544	PPI
MS.gene43544	MS.gene015255	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43544.t1	MTR_5g015570	99.315	146	1	0	1	146	1	146	6.60e-102	288
MS.gene43544.t1	MTR_2g012450	94.521	146	8	0	1	146	1	146	5.72e-89	255
MS.gene43544.t1	MTR_5g096540	91.096	146	13	0	1	146	1	146	2.36e-86	249
MS.gene43544.t1	MTR_4g126220	92.647	68	5	0	13	80	103	170	1.41e-39	131
MS.gene43544.t1	MTR_8g073140	60.465	86	7	1	30	115	1	59	8.84e-25	90.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43544.t1	AT3G49910	84.932	146	22	0	1	146	1	146	9.39e-90	257
MS.gene43544.t1	AT5G67510	85.616	146	21	0	1	146	1	146	1.20e-89	257

Find 46 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAAGTTTATCGCAGGAAA+TGG	0.331209	5.3:+8494210	MS.gene43544:CDS
CTTCCCTTCACGTCCCTTGA+AGG	0.334674	5.3:-8494183	None:intergenic
AAAACACGACGGACGCTTGA+TGG	0.398070	5.3:-8494065	None:intergenic
ATAACTCGTGAGAAGGTTAA+TGG	0.399723	5.3:+8494251	MS.gene43544:CDS
GGGTCGTGCCGCTGCTGATA+AGG	0.421050	5.3:+8494370	MS.gene43544:CDS
TACTAAGATTCGTCTGGATA+AGG	0.437481	5.3:+8494316	MS.gene43544:CDS
TGCCGCTGCTGATAAGGAAA+AGG	0.460452	5.3:+8494376	MS.gene43544:CDS
TGTGATTACTAAGATTCGTC+TGG	0.461391	5.3:+8494310	MS.gene43544:CDS
AAACACGACGGACGCTTGAT+GGG	0.465655	5.3:-8494064	None:intergenic
GCGTTCTCTTCCCGTTCGTA+AGG	0.475960	5.3:+8494133	MS.gene43544:CDS
AATGTCTTCAGGAGCAAACT+TGG	0.478032	5.3:-8494402	None:intergenic
GCAAGTTTATCGCAGGAAAT+GGG	0.483201	5.3:+8494211	MS.gene43544:CDS
AATGGGTTATTCATATTGAA+AGG	0.495637	5.3:+8494228	MS.gene43544:CDS
GAAAAGCTTGCTTGATCGTA+AGG	0.501897	5.3:+8494343	MS.gene43544:CDS
ACTTCATCATCCTTACGAAC+GGG	0.506464	5.3:-8494143	None:intergenic
TTGATGAGCGCACCTCTCTC+CGG	0.508962	5.3:+8494086	MS.gene43544:CDS
GGTTGTGAGAGGAACCTTCA+AGG	0.510507	5.3:+8494169	MS.gene43544:CDS
CACAATGAAGTTCAACCATA+GGG	0.514043	5.3:+8493998	None:intergenic
ACACAATGAAGTTCAACCAT+AGG	0.528077	5.3:+8493997	None:intergenic
GTACTTTGAACGGAGGTCAC+CGG	0.530458	5.3:-8494105	None:intergenic
GACGGCTGCTTGAAACCCTA+TGG	0.544887	5.3:-8494013	None:intergenic
CAGCCGTCGCAAGAGCCGCA+AGG	0.548428	5.3:+8494028	MS.gene43544:CDS
GCCGCTGCTGATAAGGAAAA+GGG	0.554219	5.3:+8494377	MS.gene43544:CDS
CACTTCATCATCCTTACGAA+CGG	0.557346	5.3:-8494144	None:intergenic
TCGTAAGGATGATGAAGTGC+AGG	0.559464	5.3:+8494148	MS.gene43544:CDS
GGAACCTTCAAGGGACGTGA+AGG	0.563323	5.3:+8494179	MS.gene43544:CDS
ACGACGGACGCTTGATGGGG+CGG	0.563774	5.3:-8494060	None:intergenic
AGGTTGTGCAAGTTTATCGC+AGG	0.567275	5.3:+8494204	MS.gene43544:CDS
GAGCCTTGCGGCTCTTGCGA+CGG	0.572981	5.3:-8494031	None:intergenic
CTTGCTTGATCGTAAGGCGA+AGG	0.575754	5.3:+8494349	MS.gene43544:CDS
AACGCACGTTGTACTTTGAA+CGG	0.578356	5.3:-8494115	None:intergenic
AACACGACGGACGCTTGATG+GGG	0.578903	5.3:-8494063	None:intergenic
CTTCAAGGGACGTGAAGGGA+AGG	0.593141	5.3:+8494184	MS.gene43544:CDS
TGTTGGTGTTGATCCTTCGA+AGG	0.597487	5.3:+8494286	MS.gene43544:CDS
TTAGTAATCACAACCTTCGA+AGG	0.597789	5.3:-8494299	None:intergenic
AGATTCGTCTGGATAAGGAT+AGG	0.606896	5.3:+8494321	MS.gene43544:CDS
AATGGTCAAACTGTGAATGT+TGG	0.612366	5.3:+8494269	MS.gene43544:CDS
TGAAAGGATAACTCGTGAGA+AGG	0.616719	5.3:+8494244	MS.gene43544:CDS
TTGCTTGATCGTAAGGCGAA+GGG	0.621055	5.3:+8494350	MS.gene43544:CDS
GAACGGAGGTCACCGGAGAG+AGG	0.625041	5.3:-8494098	None:intergenic
GTTGTGAGAGGAACCTTCAA+GGG	0.627782	5.3:+8494170	MS.gene43544:CDS
GAACCTTCAAGGGACGTGAA+GGG	0.643186	5.3:+8494180	MS.gene43544:CDS
GATGAAGTGCAGGTTGTGAG+AGG	0.668861	5.3:+8494158	MS.gene43544:CDS
GCACGTTGTACTTTGAACGG+AGG	0.673729	5.3:-8494112	None:intergenic
GGGCGGTGAAATGAGCCTTG+CGG	0.681827	5.3:-8494043	None:intergenic
GTGCGCTCATCAAAACACGA+CGG	0.772319	5.3:-8494076	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATGGGTTATTCATATTGAA+AGG	+	chr5.3:8494228-8494247	MS.gene43544:CDS	25.0%
	AATGGTCAAACTGTGAATGT+TGG	+	chr5.3:8494269-8494288	MS.gene43544:CDS	35.0%
	ATAACTCGTGAGAAGGTTAA+TGG	+	chr5.3:8494251-8494270	MS.gene43544:CDS	35.0%
	TACTAAGATTCGTCTGGATA+AGG	+	chr5.3:8494316-8494335	MS.gene43544:CDS	35.0%
	TGTGATTACTAAGATTCGTC+TGG	+	chr5.3:8494310-8494329	MS.gene43544:CDS	35.0%
	TTAGTAATCACAACCTTCGA+AGG	-	chr5.3:8494302-8494321	None:intergenic	35.0%
!	ACGTTTTGCATAATGTCTTC+AGG	-	chr5.3:8494416-8494435	None:intergenic	35.0%
	AACGCACGTTGTACTTTGAA+CGG	-	chr5.3:8494118-8494137	None:intergenic	40.0%
	AATGTCTTCAGGAGCAAACT+TGG	-	chr5.3:8494405-8494424	None:intergenic	40.0%
	ACTTCATCATCCTTACGAAC+GGG	-	chr5.3:8494146-8494165	None:intergenic	40.0%
	AGATTCGTCTGGATAAGGAT+AGG	+	chr5.3:8494321-8494340	MS.gene43544:CDS	40.0%
	CACTTCATCATCCTTACGAA+CGG	-	chr5.3:8494147-8494166	None:intergenic	40.0%
	GCAAGTTTATCGCAGGAAAT+GGG	+	chr5.3:8494211-8494230	MS.gene43544:CDS	40.0%
	TGAAAGGATAACTCGTGAGA+AGG	+	chr5.3:8494244-8494263	MS.gene43544:CDS	40.0%
	TGCAAGTTTATCGCAGGAAA+TGG	+	chr5.3:8494210-8494229	MS.gene43544:CDS	40.0%
!	GAAAAGCTTGCTTGATCGTA+AGG	+	chr5.3:8494343-8494362	MS.gene43544:CDS	40.0%
	AGGTTGTGCAAGTTTATCGC+AGG	+	chr5.3:8494204-8494223	MS.gene43544:CDS	45.0%
	GTTGTGAGAGGAACCTTCAA+GGG	+	chr5.3:8494170-8494189	MS.gene43544:CDS	45.0%
	TCGTAAGGATGATGAAGTGC+AGG	+	chr5.3:8494148-8494167	MS.gene43544:CDS	45.0%
	TTGCTTGATCGTAAGGCGAA+GGG	+	chr5.3:8494350-8494369	MS.gene43544:CDS	45.0%
!	ACCCTTTTCCTTATCAGCAG+CGG	-	chr5.3:8494381-8494400	None:intergenic	45.0%
!!	TGTTGGTGTTGATCCTTCGA+AGG	+	chr5.3:8494286-8494305	MS.gene43544:CDS	45.0%
	AAAACACGACGGACGCTTGA+TGG	-	chr5.3:8494068-8494087	None:intergenic	50.0%
	AAACACGACGGACGCTTGAT+GGG	-	chr5.3:8494067-8494086	None:intergenic	50.0%
	CTTGCTTGATCGTAAGGCGA+AGG	+	chr5.3:8494349-8494368	MS.gene43544:CDS	50.0%
	GAACCTTCAAGGGACGTGAA+GGG	+	chr5.3:8494180-8494199	MS.gene43544:CDS	50.0%
	GATGAAGTGCAGGTTGTGAG+AGG	+	chr5.3:8494158-8494177	MS.gene43544:CDS	50.0%
	GCACGTTGTACTTTGAACGG+AGG	-	chr5.3:8494115-8494134	None:intergenic	50.0%
	GCCGCTGCTGATAAGGAAAA+GGG	+	chr5.3:8494377-8494396	MS.gene43544:CDS	50.0%
	GGTTGTGAGAGGAACCTTCA+AGG	+	chr5.3:8494169-8494188	MS.gene43544:CDS	50.0%
	GTACTTTGAACGGAGGTCAC+CGG	-	chr5.3:8494108-8494127	None:intergenic	50.0%
	GTGCGCTCATCAAAACACGA+CGG	-	chr5.3:8494079-8494098	None:intergenic	50.0%
	TGCCGCTGCTGATAAGGAAA+AGG	+	chr5.3:8494376-8494395	MS.gene43544:CDS	50.0%
	AACACGACGGACGCTTGATG+GGG	-	chr5.3:8494066-8494085	None:intergenic	55.0%
	CTTCAAGGGACGTGAAGGGA+AGG	+	chr5.3:8494184-8494203	MS.gene43544:CDS	55.0%
	CTTCCCTTCACGTCCCTTGA+AGG	-	chr5.3:8494186-8494205	None:intergenic	55.0%
	GACGGCTGCTTGAAACCCTA+TGG	-	chr5.3:8494016-8494035	None:intergenic	55.0%
	GCGTTCTCTTCCCGTTCGTA+AGG	+	chr5.3:8494133-8494152	MS.gene43544:CDS	55.0%
	GGAACCTTCAAGGGACGTGA+AGG	+	chr5.3:8494179-8494198	MS.gene43544:CDS	55.0%
	TTGATGAGCGCACCTCTCTC+CGG	+	chr5.3:8494086-8494105	MS.gene43544:CDS	55.0%
	GGGCGGTGAAATGAGCCTTG+CGG	-	chr5.3:8494046-8494065	None:intergenic	60.0%
	ACGACGGACGCTTGATGGGG+CGG	-	chr5.3:8494063-8494082	None:intergenic	65.0%
	GAACGGAGGTCACCGGAGAG+AGG	-	chr5.3:8494101-8494120	None:intergenic	65.0%
	GAGCCTTGCGGCTCTTGCGA+CGG	-	chr5.3:8494034-8494053	None:intergenic	65.0%
!	GGGTCGTGCCGCTGCTGATA+AGG	+	chr5.3:8494370-8494389	MS.gene43544:CDS	65.0%
	CAGCCGTCGCAAGAGCCGCA+AGG	+	chr5.3:8494028-8494047	MS.gene43544:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	8494002	8494442	8494002	ID=MS.gene43544
chr5.3	mRNA	8494002	8494442	8494002	ID=MS.gene43544.t1;Parent=MS.gene43544
chr5.3	exon	8494002	8494442	8494002	ID=MS.gene43544.t1.exon1;Parent=MS.gene43544.t1
chr5.3	CDS	8494002	8494442	8494002	ID=cds.MS.gene43544.t1;Parent=MS.gene43544.t1

Gene Sequence

>MS.gene43544

ATGAAGTTCAACCATAGGGTTTCAAGCAGCCGTCGCAAGAGCCGCAAGGCTCATTTCACCGCCCCATCAAGCGTCCGTCGTGTTTTGATGAGCGCACCTCTCTCCGGTGACCTCCGTTCAAAGTACAACGTGCGTTCTCTTCCCGTTCGTAAGGATGATGAAGTGCAGGTTGTGAGAGGAACCTTCAAGGGACGTGAAGGGAAGGTTGTGCAAGTTTATCGCAGGAAATGGGTTATTCATATTGAAAGGATAACTCGTGAGAAGGTTAATGGTCAAACTGTGAATGTTGGTGTTGATCCTTCGAAGGTTGTGATTACTAAGATTCGTCTGGATAAGGATAGGAAAAGCTTGCTTGATCGTAAGGCGAAGGGTCGTGCCGCTGCTGATAAGGAAAAGGGTACCAAGTTTGCTCCTGAAGACATTATGCAAAACGTTGATTAG

Protein sequence

>MS.gene43544.t1

MKFNHRVSSSRRKSRKAHFTAPSSVRRVLMSAPLSGDLRSKYNVRSLPVRKDDEVQVVRGTFKGREGKVVQVYRRKWVIHIERITREKVNGQTVNVGVDPSKVVITKIRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQNVD